KR101029659B1 - Attraction trap for both behavioral and biological pest control and the method using it - Google Patents

Abstract

The present invention relates to a pest control technology that combines biological control to inhibit the density of pests by inducing natural natural proliferation to behavioral control through pest attraction. In the present invention, (a) a attracting trap for attracting together the pests and natural enemies parasitic in the eggs of the pests; (b) an inactivated egg of the pest supplied with the attractant trap is provided. The pest control technology of the present invention can obtain the effect of biological control without costly proliferation and radiation of natural enemies, it is environmentally friendly because there is no environmental toxicity, even if the number of pesticide spraying is less than the economic damage tolerance level This can reduce the overall control costs, increase farm income, and reduce environmental pollution.

Pheromone traps, natural enemies, host eggs, inactivated eggs, biological control, cold storage, stink bug

Description

Attract trap for both behavioral and biological pest control and the method using it}

The present invention relates to a manned trap for controlling pests through pest attraction and a method for controlling the same, in particular, a control that combines biological control to suppress the density of pests by inducing natural natural proliferation to behavioral control through attraction. It's about technology.

Recently, absorbent stink bugs have emerged as important pests in food crops such as rice, barley and soybeans, and in fruit crops including apples, persimmons and pears. They feed on the seeds and fruits of crops by pricking and sucking them, so they directly add the target products of agricultural production, causing great economic damage, and having high mobility, making it difficult to control insecticides.

Known methods for controlling stink bugs other than insecticides include (1) behavioral stabilization using attracted traps using their aggregated pheromones, and (2) biological control by parasitic bees parasitic on stink bug eggs. In particular, the area of eco-certified agricultural land is increasing to 122,882 ha as of 2007 due to changes in the consumption pattern of consumers who prefer safe agricultural products (Agricultural Product Quality Management Service 2007, http://www.naqs.go.kr). The method has attracted attention as an environmentally friendly pest control method. The method of the above (1) is already known in the domestic patent registration No. 10-0853616 and is widely used by commercializing as a trap type attracting trap from Green Agrotech Co., Ltd. The method of (2) using natural enemies has been successfully used in many countries such as the United States, Japan, and Brazil (Orr 1988, Fla. Entomol. 71: 506-527), but it is difficult to apply them in Korea. The reason for this is that (1) stink bugs are mainly generated from open field crops, and therefore, cost is inferior due to the high cost of growing a large number of natural enemies. This is because in many cases, the effect is inconsistent due to a lack of adaptability. Therefore, at present, it is difficult to mass-produce and radiate natural enemies for the control of stink bugs. Therefore, rather than radiating natural enemies, it is using a method of conservation through conservation of natural enemies. Examples include the use of selective pesticides that are not toxic to natural enemies, and the placement of plants around crops that can provide natural food (pollen and honey). A method of supplying direct hosts has also been proposed (Peng et al. 1984, Acta Entomol. Sinica 27, 39-47; Mahmoud and Lim 2008, Biannual Meeting of Korean Soc. Appl. Entomol. At Muju). Among them, Mahmoud and Lim (2008) showed that stink eggs stored at low temperature are inactivated and the stink bugs do not hatch, but parasitic bees can be parasitic normally without negative effects. A study found that the increase and the stink bug damage were reduced.

In addition, it has been found that not only stink bug adults and nymphs but also natural parasitic bees are attracted to the trap of stink bugs using conventional pheromone (Leal et al. 1995, J. Chem. Ecol. 21, 973-985; Huh and Park 2005, Korean J. Appl. Entomol. 44, 131-137). The stink bugs are trapped, but the parasitic bees are much smaller than the stink bugs. However, this was not significant because parasitic bees do not parasitic stink bugs and nymphs. If a parasitic bee stays in a trap to find stink bug eggs, it is impossible to parasitic stink bug eggs in the actual package, so there may be a problem in that the use of traps lowers the parasitic rate due to natural enemies.

According to the present invention, stink bugs are attracted to the traps of stink bugs using aggregated pheromones, as well as stink bugs and nymphs, and stink parasites of stink bugs are not hatched from stink bugs. Parasitic bee) is a parasitic normal to focus on the development and allegory, in the present invention, the behavioral control (Behavioral control) through the attraction of stink bugs and the biological control (Biological control) through the attraction and proliferation of natural enemies at the same time To provide pest control technology that can be done.

To this end, in the present invention, by utilizing the point that the natural enemy is attracted to the stink bug attracting trap using the aggregated pheromone, parasitic eggs supplied to the induced natural parasitic bees by supplying inactivated host eggs stored at low temperature in the trap By raising the parasitic bees that have completed their development, the parasite bees will be increased in density and overall parasitic rate so that behavioral control using manned traps and biological control through inducement and propagation of natural enemies are carried out simultaneously. In addition, the present invention demonstrates this through experiments in actual outdoor packaging.

Specifically in the present invention,

(a) an attracting trap that attracts the pest together with the parasitic nemesis in the egg of the pest;

(b) an inactivated egg of the pest supplied with the attractant trap is provided.

In addition, the present invention provides a pest control method using the above-mentioned pest and natural enemy traps to attract natural enemies parasitic from pests and pest eggs and increase the parasitic rate and density of the natural enemies.

In the present invention, by combining the (1) the point that the natural enemy is attracted to the stink bug attracting trap using the conventional pheromone and (2) that the parasitic nature of the stink bug is normally parasitic, development, allegory to the low temperature stored host eggs We proposed a new concept of pest control that combines not only behavioral control through attraction but also biological control through attraction and propagation of natural enemies. Proved.

Insect traps and natural enemies of the present invention by adding a natural enemy control effect to the effect of the existing trap, induces natural natural proliferation along with behavioral control of the pest to suppress the density of the pest to maximize the pest control effect Done. The control technique of the present invention can (1) obtain the effect of biological control without the costly multiplication and radiation of natural enemies, (2) environmentally friendly, there is no environmental toxicity, and (3) reducing the number of spraying pesticides The density of stink bugs can be kept below the level of economic damage. This leads to a reduction in overall control costs by reducing the use of pesticides, cost savings and increased productivity of crops leads to an increase in farm household incomes, and furthermore, protection of the environment by reducing the use of pesticides to reduce soil and water pollution. Will have

The pest and natural enemy attracting trap of the present invention attracts both the pest and the natural enemy parasitic in the egg of the pest, and the inactivated eggs of the pest are supplied together.

In the present invention, "inactivated egg" refers to a host egg that is stored at low temperature so that the host does not hatch, but the parasitic nemesis is normally parasitic to develop and allegorize.

In the present invention, "supplied together" means that the inactivated eggs are placed in the manned trap as it is, or in a separate container, or when the container containing the eggs is installed on one or more of the top, bottom, left, and right sides of the manned trap. Of course, including the case where the container containing the egg is installed adjacent to the attracting trap. At this time, the range that can be installed adjacent to the limit to the range that the attracting effect of the attracting trap.

The inactivated eggs supplied with the attractant traps are preferably contained in a separate container, where the separate container can contain the eggs regardless of the material or shape, and all of them can be used freely. Possible, for example, plastic containers, paper boxes, plastic bags, bags and the like can be used. In order to prevent the infestation of eggs inactivated by other predators such as spiders, the container may have a form that allows natural enemies to pass freely while other pests do not. In one embodiment of the present invention, a bag containing eggs is made of a cloth having a pore size through which only parasites can pass freely.

The pest and natural enemy attracting trap of the present invention may include the aggregated pheromone of the pest so as to attract the pest and natural enemy together. It is well known to attract pests and their natural enemies by such aggregated pheromones, and aggregated pheromones that can be used for this purpose are also known techniques. The pheromone trap of the sawtooth ant waist lining material selected as a model in the embodiment of the present invention is known in the domestic patent registration No. 10-0853616, currently commercialized as a trap type attracting trap from Green Agrotech Co., Ltd. Commonly used in food crops and fruit crops.

In addition, the attraction trap may include a natural enemy food to increase the natural attraction effect. In addition to the attracting trap of the present invention, a known pest capable of attracting the target pest and the natural enemy of the pest can be applied by the choice of those skilled in the art.

The control technique of the present invention may be mainly applied to the stink bug pests, but is not limited thereto, and may be any pest that is conceptually applicable to the control technique in which the behavior control and the biological control proposed in the present invention are fused. In an embodiment of the present invention, the top bridge ant waist norinjae (Riptortus clavatus) and its natural enemies of hop acids jombeol gisaengbeol (Ooencyrtus nezarae) of norinjae pest it was applied to the model. Ooencyrtus nezarae , the natural enemy of the stinging ants, is distributed in Japan, Korea, and China, and has been found to parasitic on about 11 kinds of stinging eggs. In Korea, the stinging ants, along with the black bee parasitic bee ( Gryon japonicum ) It is the main natural enemy of stink bugs. The fact that it is attracted by the aggregated pheromone of the sawtooth antling stink bug, the host of the Ooencyrtus nezarae , is reported by Leal et al. (1995, J. Chem. Ecol. 21, 973-985).

The pest and natural enemy attracting traps of the present invention were installed in actual outdoor packaging in the following examples, and the effects were tested. As a result of experiments with four soybeans placed on the edges, we found that the parasitic rate of stink bugs by parasitic bees and the number of stink bugs and eggs were reduced, and the percentage of soybean pods damaged by stink bugs was also reduced. It was found to be effective. As described above, the pest control technology of the present invention is made by simultaneously controlling the pest (Behavioral control) with the action (Bhavioral control) to attract the natural enemies and induces the proliferation by controlling the biological control (Biological control) to suppress the density of the pest (pest control effect To maximize.

[Example]

Hereinafter, the present invention will be described in more detail with reference to specific examples. However, these examples are only for illustrating the present invention in more detail, the scope of the present invention is not limited by these examples.

Production Example

The insect pests of the present invention are attached by attaching a bag containing stink bug eggs so that Ooencyrtus nezarae, which is attracted like stink bugs, can be parasitic on the side of the claw- type ant loin norin material for the control of cultivation type sawtooth ants that are sold by Green Agrotech Co., Ltd. A natural manned trap was completed (FIG. 1). The bag containing stink bug eggs was made of a cloth sized to allow only free passage of the parasitic bee , Ooencyrtus nezarae , to prevent the stinger eggs from being fed to other arthropod predators. The egg of the stink bug used was stored for 1 month at 2 ° C. to prevent the stink bug from hatching.

Control experiment

In 2008, three environmentally friendly soybeans (treatment) of Dosan-myeon, Andong-si, were installed with four pests and natural enemy attracting traps of the present invention manufactured in the manufacturing example per package, and three other green soybeans were installed with only stink bugs. The cultivation (control) and parasitic rates, stink bug density and damage rate of soybean pods were compared. Areas of the three treatment zones were 3000, 4600, and 3300m2, respectively, and the varieties of soybean were Seoritae, Seomoktae, and Daewon soybean. Areas of the three control plots were 2000, 3300, and 2000m2, respectively, and the varieties of soybean were Seoritae, Seomoktae, and Celadon bean. The distance between the pavements was at least 200 meters away. In the treatment area, a total of 10,000 eggs were divided and installed in four traps three times for each package.In the 2nd and 3rd spinning, the previously released eggs were taken out and transferred to a separate egg bag, I hung my support stand in the middle point. The eggs were released every 18 days after the initial spinning (August 17, 2008), and 50 soybeans were randomly selected from each package every 9 days to compare changes in stink bug density and parasitic rate in the package ( However, the date of investigation was changed in case of inevitable due to weather conditions). The nymphs and adult insects of stink bugs were recorded in the field, and the eggs were brought to the laboratory and examined for parasitics. In addition, the pests and natural attraction traps were installed in the control group to compare the parasitic rate in the radiated eggs. However, only 30-50 eggs per trap were spun and collected. It was. To compare the difference in damage caused by stink bugs, the method of Bowling (1979, J. Econ. Entomol. 72, 259-260) was randomly collected at 60 weeks when the bean pods were in hypertrophic growth phase. The stylet sheath of stink bugs was investigated.

The results are shown in FIGS. 2 to 7. In the figure, "D -Sm-T" is Dosan-Seomok-Treatment, "D-Sr-T" is Dosan-Surtae-Treatment, "D-Dw-T" is Dosan-Daewon-Treatment, "D-Sm- C ”is Dosan-Seomoktae-Daegu,“ D-Sr-C ”is Dosan-Seotae-Daegu, and“ D-Ch-C ”is Dosan-Chungja-kong-Daegu.

The results showed that the parasitic rates of eggs collected and packaged in the package showed a variety of changes in the early stages of treatment, but the control group (0 ~ 62%) in the latter part of crop cultivation (9/12 ~ 10/8). ) Was higher in the treatment group (78-91%) (Fig. 5; χ 2 = 97.136, df = 5, P <0.001). In addition, the same tendency was confirmed in the stink bug eggs radiated to the egg sacs after cold storage (FIG. 6; χ 2 = 231.000, df = 5, and P <0.001). In particular, the feeding traces found in the pods were significantly lower in the treatment group (12-25%) than in the control group (25-42%) (FIG. 7; χ 2 = 59.579, df = 5, P <0.001). In the variability of stink bugs, the larvae ( Hc = 11.277, df = 5, P = 0.046) and nymphs ( Hc = 11.455, df = 5, P = 0.043), eggs ( Hc = 14.352, df = 5, P = 0.014) all remained generally low in the second half (Figs. 2, 3, 4). From the above results, it was confirmed that the host eggs fed to the stink bug attracted the parasitic rate of parasitic bees, which decreased the density of stink bugs in the pavement and the damage rate of bean pods.

1 is a photograph of a pest and natural enemy attracting trap of the present invention in which a bag containing stink bug eggs is attached to an attracting trap of Riptortus clavatus .

2 is a control (D-Sm-T, D-Sr-T, D-Dw-T) and the control (D-Sm-C, D-Sr-) equipped with a pest and natural enemy trap of the present invention C, D-Ch-C) is a graph showing the density variation of stink bug adult.

Figure 3 is a graph showing the density fluctuation of the stink bug nymph appeared in the control and non-treatment in which the pest and natural enemy attracting trap of the present invention.

Figure 4 is a graph showing the density fluctuation of the stink bug eggs in the control and non-treatment in which the pest and natural enemy attracting trap of the present invention.

5 is a graph showing the variation of the parasitic rate due to the parasitic bees of stink bug eggs collected from the control and non-control control with a pest and natural enemy trap of the present invention.

FIG. 6 is a graph showing the variation of the parasitic rate of the parasitic bees for the inactive stink bug eggs in the bag attached to the trap in the control and non-control groups equipped with the pest and natural enemy trap of the present invention.

FIG. 7 is a graph showing variation in the rate at which stylet sheaths were detected by stink bugs of soybean pods collected from the treated and non-controlled pests and natural enemies.

Claims (6)

(a) a lure trap that attracts stink bugs and nemesis parasitic from the eggs of the pest; (b) inactivated eggs of the pests supplied with the attractant traps; The deactivated egg is trapped in stink bugs and natural enemies, characterized in that the natural enemy is free to pass while the pest is contained in a container that can not pass. The attractant trap according to claim 1, comprising an aggregated pheromone of the pest. The attracting trap according to claim 1, comprising a predator of natural enemies. delete delete A method for controlling a hemipteran pest, comprising installing a hemipteran pest and a natural attraction trap of claim 1, attracting a hemipteran pest and a natural enemy parasitic from the eggs of the pest, and increasing the parasitic rate and density of the natural enemy.

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