CN105994213B - Insect trapping and killing device and method - Google Patents

Insect trapping and killing device and method Download PDF

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Publication number
CN105994213B
CN105994213B CN201610341965.6A CN201610341965A CN105994213B CN 105994213 B CN105994213 B CN 105994213B CN 201610341965 A CN201610341965 A CN 201610341965A CN 105994213 B CN105994213 B CN 105994213B
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attractant
pheromone
releaser
insect
trapping
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CN105994213A (en
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陈超
塞斯文
高爱华
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Jiangsu Fengyu Biotechnology Co., Ltd
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Nanjing Xin'an China Green Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/02Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/20Poisoning, narcotising, or burning insects

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Engineering & Computer Science (AREA)
  • Insects & Arthropods (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)

Abstract

The invention relates to an insect trapping and killing device, comprising: a. an attractant; b. a releaser for carrying an attractant; c. an insecticide coated on the outer surface of the releaser; wherein the attractant comprises one or more of insect pheromone, plant volatile attractant and amine-containing attractant, and also comprises a slow-release carrier. This trapping and killing device compares and disturbs mating technique, and the pheromone that needs to use is few, and the pheromone releaser's that needs to use quantity is low, saves cost and manual work.

Description

Insect trapping and killing device and method
Technical Field
The invention belongs to the field of agriculture, and particularly relates to an insect trapping and killing device and an insect trapping and killing method.
Background
Peach fruit borer (Carposina sasakii) eats delicious fruits, is a main pest of apples and jujubes produced in China, Korea and Japan, and also has damage to plums, pears, apricots and peaches in some areas. Peach fruit borers occur in late spring until autumn. Peach fruit borer larvae can dig into the fruit for damage and are therefore difficult to control by pesticides.
Apple leafworm (Adoxophyes orana) is a type of leafworm that is widely distributed in Asia and Europe and is a hazard to a wide variety of fruit crops. Leaf roller moth larvae feed on the leaves and pulp, are extremely harmful to apples and may occur simultaneously with peach fruit borers in orchards.
In the world, codling moth (Cydia pomonella) is a main pest of apples, pears and walnuts, and the codling moth larvae can cause damage after drilling into fruits and are fruit-eating pests, and are difficult to control through pesticides to influence the income of fruit growers.
Chilo suppersalis (Chilo suppersalis) is a main pest of rice in the whole China, and is particularly serious in northeast China; corn borer (Ostrinia furnacalis) is a main pest on Chinese corn, and the two pests continuously and irregularly occur from the middle ten days of May to October, so that farmers are difficult to master the accurate pesticide spraying time, only can continuously use a large amount of pesticides, pests resist the pesticide, the labor of the farmers is increased, and the excessive use of the pesticides causes the pesticide residue to exceed the standard and causes pollution to the environment.
The sweet potato weevil (Cylas formicarius) is a weevil, is a main pest on sweet potatoes worldwide, has an active period of 6 to 8 months in one year, mainly takes tubers as food, is difficult to control and has high cost; as long as the fruit is infested with this pest, it becomes stale and unmarketable.
The sweet potato weevil generates 6-8 generations in Guangdong one year, and has obvious generation overlapping phenomenon. The adult overwintering method mainly comprises the steps of overwintering in the field and in the stored potato blocks and in hidden positions such as stems, leaves and soil seams; eggs, larvae and pupae can also overwinter in potato pieces. The early spring adult insects firstly finish 1 generation on the overwintering plant and then are transferred to the field for harm. The imagoes have weak flying ability, are afraid of direct sunlight and have false death. Eggs are scattered below the cortex of the potato tuber, and then are laid on the thicker potato tendrils, the egg laying hole is generally covered with colloid, and each female can lay 30-200 eggs. The whole larval stage lives in the potato blocks or the vine heads, the interior of the potato blocks is eaten by the larvas to form an unshaped bent tunnel, the tunnel is filled with insect feces, the damaged potato blocks are stink and bitter due to the invasion of pathogenic bacteria caused by wounds, and the aged larvas pupate at the position close to the surface layer of the tail end of the tunnel.
Anoplophora chinensis (anophophora chinensis) is a large-scale long horn beetle, which damages the trunks of citrus and certain nut trees, and also damages ornamental trees. Anoplophora glabripennis (Anoplophora glabripennis) is a major pest of forest and ornamental trees in china.
Coffee Kazu-shaped longhorn beetles (xylotreuchus quadripes) are longicorn beetles which can damage Yunnan Arab coffee trees and cause death of the trees, and are a potential threat to the coffee industry.
Drosophila, especially oriental fruit fly (Bactrocera dorsalis), melon fly (Bactrocera cucurbitae) and citrus fruit fly (Bactrocera minax) are important pests of fruits, melons and certain vegetables in china. They like the taste of certain amines, which is the taste of food for adult fruit fly.
Empoasca vitis, a semipteroid insect, damages Chinese grapes and tea leaves by sucking the sap of the leaves and soft stems. Empoasca vitis preferring tea plant volatiles including trans-2-hexenal, cis-3-hexen-1-ol, geraniol, linalool and trans-ocimene.
For different pests, it may be good if the effective pesticide can be sprayed timely and accurately, but the harm of spraying a great amount of pesticide is still very much, for example: 1. the health of the user is harmed; 2. pesticide residues may be left on the fruits; 3. inevitably, spraying a large amount of insecticide will kill a large number of beneficial insects, resulting in outbreaks of secondary insect pests, such as tetranychus urticae, leaf miner on fruit trees and rice planthopper; 4. the requirement for labor is increased by frequently spraying the insecticide, and the production cost is increased; 5. crops usually growing on hillsides, such as jujube, are inconvenient to spray with insecticide; 6. polluting water source and harming environment.
The peach fruit borer female insects release a sex pheromone in the mating period to attract the peach fruit borer male insects to mate, so that the reproduction of the peach fruit borer female insects depends on the communication method. The sex pheromone of peach fruit borer has two components: z, 13 Eicosen-10-one (Z, 13 Eicosen-10-one) and Z, 7 eicosatriene (Z, 7 Tricosene).
The peach fruit borer can be induced by using Z, 13 Eicosen-10-one (Z, 13 Eicosen-10-one) or by mixing Z, 13 Eicosen-10-one (Z, 13 Eicosen-10-one) and Z, 7 eicosatriene (Z, 7 Tricosene). In general, during monitoring, the lure core containing the sex pheromone is placed in a monitoring box with a sticky plate at the bottom, and the male peach fruit borer is attracted by the sex pheromone of the peach fruit borer released by the lure core to fly into the monitoring box and is stuck by the sticky plate. The monitoring box is suitable for evaluating the quantity and occurrence condition of the field quantity of the peach fruit borers, but if a mating interference method is adopted and is used in a large quantity as a method for controlling the peach fruit borers, the pheromone is too expensive, so that the product price is higher.
The use of Z, 13 Eicosen-10-one alone (Z, 13 Eicosen-10-one) or a mixture of Z, 13 Eicosen-10-one (Z, 13 Eicosen-10-one) and Z, 7 eicosatriene (Z, 7 Tricosene) can control the peach fruit borer hazard by disrupting mating. Interference of the male moths with chemically synthesized sex pheromones does not allow the true female moths to be found, and mating does not occur unless it is met by chance. The mating interference method is closely related to the population base number in the orchard, and when the quantity of the peach fruit borers in the orchard is large, the mating interference technology is used and must be matched with a proper pesticide to reduce the insect pressure. In the case of disturbed mating, since male moths will persist, real female moths are sought after one time until after several days to exhaust energy.
Therefore, the possibility that the male moth meets the female moth still exists, and the machine rate is higher than that of a system for directly trapping and killing the male moth, because in the direct trapping and killing system, the male moth only has one chance to die, the trapping and killing rate is improved, and the mating probability is further reduced. However, in the past, because the attractant for the pests has short duration, the labor cost is high when the attractant is frequently replaced, and in addition, the price of the used monitoring trapping box or equipment is not suitable for large-scale commercial use and is too high, the prevention and control technology which can be used for preventing and controlling the pests in a large area is not available.
The mating disruption technique of peach fruit borer (see chinese patent CN102823590A) uses 1500 (100/acre) sex pheromone divergers per hectare, and each diverger contains 65 mg of sex pheromone. Therefore, 97.5 g pheromone (6.5 g/mu) is needed to be used per hectare, and the cost of the peach fruit borer sex pheromone is high, so that farmers cannot afford the technology, and the technology cannot be popularized commercially.
Female apple leaf rollers release a pheromone consisting of cis-9-tetradecene acetate (Z, 9 Tetradecen-1-yl acetate) and (Z, 11 Tetradecen-1-yl acetate) in a ratio of 4: 1. Female moths use this pheromone to attract male moths to mate. Like carposina niponensis, the pheromone of the apple leafroll moth can be used to control release devices to control this pest by disrupting mating. When pheromone-disrupted mating technology was used to control such insects, a minimum of 33 dispensers containing 250 mg of pheromone per acre were used. Thus, 125 grams of pheromone (8.3 grams/acre) per hectare is required. Like the sex pheromone of the peach fruit borer, the price of the pheromone of the apple leaf roller is very expensive, and farmers can hardly bear the pheromone. For fruit growers planting apples, apple leaf roller moth pheromone and peach fruit borer pheromone are needed to be used at the same time, so that the cost is higher, and the large-area commercial popularization and use cannot be realized.
The female codling moth releases a pheromone with a component of trans, trans-8, 10-Dodecadien-1-ol (E, E8, 10 Dodecadien-1-ol). This pheromone is widely used to control codling moth by interfering with mating in europe, north america, south africa and australia. Currently, china has not controlled the damage of codling moth by using this pheromone. Mating disruption of codling moth requires 200 grams of active ingredient per hectare (13.3 grams/acre).
The semiochemicals released by the female chilo suppressalis comprise cis-11-hexadecenal (Z, 11 Hexadecen-1-al), cis-13-hexadecenal (Z, 13 Hexadecen-1-al) and cis-9-hexadecenal (Z, 9 Hexadecen-1-al) in a ratio of 48: 6: 5. This pheromone was used experimentally to control chilo suppressalis at 6-7.5 grams per hectare. Pheromones have also been used for trap control at rates of 4 and 10 mg per hectare (0.27 and 0.67 per acre).
The female Asiatic corn borer (Ostrinia furnacalis) produces a pheromone consisting of (Z) -12-tetradecenyl acetate (Z, 12 Tetradecen-1-yl acetate) and (E) -12-tetradecenyl acetate (E, 12 Tetradecen-1-yl acetate) in a ratio of 1: 1 to 2: 1. The pheromone is experimentally used for controlling chilo suppressalis by matching with an insecticide through an interference mating method, and the using amount of the pheromone per hectare in the season is 50-150 mg (3.3-10 mg per mu). The use of pheromone monitoring boxes (together with the insecticide) was also used for trap control at a rate of 4 and 10 mg per hectare (0.27 and 0.67 per acre).
Both female and male flies of the genus drosophila are attracted by the smell of amine-containing food and can be used as one of the attractant components for attracting fruit flies effectively.
The female weevil produces a very potent pheromone (Z) -3-12 alkenyl ester (Z, 3 Dodecenyl butenoate) for attracting male weevils. Such pheromones have been used to trap control using a large number of traps.
The sex pheromone is prepared by mixing 4-n-heptyloxybutanol (4- (n-heptyloxyl) -1-ol) and 4-n-heptyloxybutyraldehyde (4- (n-heptyloxyl) -1-al) in a ratio of 1: 1, and can attract female insects and male insects. Pheromones released from Anoplophora chinensis (Anoplophora chinensis) contain only 4-n-heptyloxybutanol (4- (n-heptyloxyxy) -1-ol).
The male Bulbophyllum caucasianum which is deinsectization coffee produces pheromone with the component of (S) -2-hydroxy-3-decanone, and can attract female insects and male insects at the same time.
The sweet potato weevil, the anoplophora glabripennis and the coffee deity tiger longhorn are not objects of mating interference, but can be objects trapped and killed in a large quantity.
From the above, it can be seen that the use of mating disruption techniques achieves a hazard control effect similar to but more often than that achieved with insecticides alone. However, since pheromones are expensive and used in large quantities, the cost is difficult to reduce.
In the prior art, other trapping and killing technologies are used for preventing and controlling codling moth (codling moth) and Chilo suppersalis (Chilo suppersalis), but the trapping and killing are mainly performed by using sticky glue, the main purpose is monitoring, the sticky plate needs to be replaced frequently, the use cost and the labor cost are high, and the insect trapping and killing are not complete.
Disclosure of Invention
It is an object of the present invention to overcome the disadvantages of the prior art and to provide an insect trapping and killing device which requires less pheromone than the interferential mating technique, requires a lower number of pheromone releasers and is cost and labor saving. Because the male moths can be killed once the male moths fall on the device used by the trapping and killing mechanism, the male moths can be killed only by appearing once, and the efficiency is improved. The trapping and killing device is provided with a bearing object, the pesticide is only used on the surface of the bearing object, does not contact fruits, does not need to be sprayed in an orchard comprehensively, has the advantage of no residual pesticide on products, and has the advantages of small dosage of the pesticide used in the trapping and killing system, safety and environmental protection, and only needs to be used once a year.
The other object of the present invention is to provide a method for attracting and killing insects.
The insect trapping and killing device comprises: a. an attractant; b. a releaser for carrying an attractant; c. an insecticide coated on the outer surface of the releaser;
The attractant comprises one or more of insect pheromone, plant volatile attractant or amine-containing attractant, a slow release carrier, an antioxidant, an ultraviolet-resistant stabilizer and a solvent, wherein the slow release carrier can be rubber head, wood powder, wax, vermiculite, perlite, silica gel, clay and the like.
Wherein when the attractant adopts insect pheromone, the weight ratio of the insect pheromone to the antioxidant to the ultraviolet-resistant stabilizer to the slow-release carrier in the attractant is (1-10) to (1-2) to (0.5-1.0) to (2000-3000); wherein the insect pheromone is selected from one or more pheromones of peach fruit borer, codling moth or other moth family Olethritiniae species, or Chilo suppressalis, Asian corn borer (Ostrinia furnacalis) or other Crambidae family Cnaphalocrocodiella species, or sweet potato weevil (Cylas formicarius), adult beetles including but not limited to Astrocarya (Anoplophora) or Kazu Heterophyllus (Xylotreus quadripes). When the solvent is added, the formula proportion of the attractant is that the weight ratio of the insect pheromone, the antioxidant, the ultraviolet-resistant stabilizer, the solvent and the slow-release carrier is (1-10): (1-2): (0.5-1.0): (400-.
Wherein the antioxidant is preferably Butylhydroxytoluene (BHT), and the solvent is selected from pentane and vegetable oil.
When the attractant is plant volatile attractant, the plant aromatic of Empoasca vitis is preferably used.
Wherein the weight ratio of the plant volatile attractant to the antioxidant to the ultraviolet-resistant stabilizer to the sustained-release carrier in the attractant is (700-.
Wherein the attractant can also adopt amine-containing attractant which is attractive to fruit fly (Bactrocera), the weight ratio of the amine-containing attractant substance to the slow-release carrier is (20-80) to (80-20), the amine-containing attractant is preferably ammonium acetate, the carrier is one or more selected from vermiculite, perlite, silica gel, clay and wood powder, and each releaser is preferably filled with 10-30 mg of attractant.
The pheromone has hydrophobicity, and can be dissolved in hydrophobic carriers such as wax, rubber and the like. In contrast, the amine-containing compounds in the fruit fly attractant are highly hydrophilic, and are very inconvenient to use alone or directly due to water absorption and liquefaction in the air, and we find that the amine-containing compounds can solve the problem by mixing with carriers such as cellulose, wood powder, bamboo powder, vermiculite, perlite, silica gel or clay, and the like, and subpackaging the mixture into small bags according to needs, so that the content can be ensured, and the stability of the amine-containing attractant can be ensured, and we have found that the high-density polyethylene synthetic paper meets the packaging requirements of the mixture.
The attractant-bearing releaser can be a plate, a container, a bag or a material capable of being wound on the branches of trees.
The attractant-bearing releaser of the invention is preferably a bag made of high-density polyethylene synthetic paper (spin-bonded polyethylene), polypropylene fiber (non-woven fabric), PVC (less than 0.5 mm), polyethylene/starch board (less than 0.5 mm), cotton fabric (cloth) or PLA (polylactic acid) polylactic acid non-woven fabric, and the materials have high strength and good waterproofness, and the pores of the materials are enough to release the attractant in the bag.
The attractant-carrying release of the present invention may also be a container with one, two or more holes.
The container may be, for example, a plastic bottle, a trap barrel, a waterproof bag, a waterproof box, a conical container, various containers made of cardboard, and the like, but is not limited thereto. All containers must have water resistance and resistance to uv oxidation. As an example, a disposable plastic container made of a material having waterproof property and ultraviolet ray oxidation resistance can be used in consideration of convenience. Moreover, whatever the container, it must be able to satisfy the feature of remaining intact without being affected by the weather, placed in the orchard for several months in succession. By way of example, it may be 1-5 months, or more. This is also the main reason why the container of the present invention is made of a material having water-repellent properties and ultraviolet ray oxidation-resistant properties.
The releaser is preferably economic and transportable, the flat package of the device can effectively utilize the storage and transportation space, and the degradability and environmental protection are also important to consider; meanwhile, under the environment of sunshine, wind and rain, the tree trunk is hung on the tree trunk or exposed in the field, and the integrity and the functionality of the tree trunk are ensured for at least not less than one month. This requires that the device be uv protected and strong enough not to be damaged and fall out under strong winds.
The outer surface area of the release is preferably 24-1536 square centimeters and the outer surface of the release in the device must be such as to attract a large number of insects falling and contacting the insecticide on its surface. When the surface area of the releaser is larger than 1536 square centimeters in rating, the insect-proof effect can be more effective, but the increase of the surface area of the bearing object can be restricted by factors such as cost, convenience and the like.
The insecticide is insoluble in water and can be a synthetic pyrethroid insecticide, preferably, the insecticide is a pyrethroid insecticide, including but not limited to: deltamethrin, alpha-cypermethrin, beta-cypermethrin, bifenthrin and fenvalerate, preferably deltamethrin.
These insecticides are commercially available. It should be noted that the insecticide applicable to the present invention is not limited to the above-described ones, and any insecticide having the following functional characteristics can be used as long as it can be coated on the outer surface of the container. The functional features include: a. the selected insecticide is effective on target insects, and has the characteristic of rain wash resistance and lasting effect for more than 1 month; c. the selected insecticide is not easily decomposed by ultraviolet rays; d. the selected pesticide has low toxicity to human skin.
The insect trapping and killing device provided by the invention can attract insects by the volatile attractant to the releaser carrying the attractant, and then the insects contact with the outer surface of the releaser coated with the insecticide to achieve the trapping and killing purpose. Since the outer surface of the releaser is coated with the insecticide, the insects can be killed even if they do not enter the releaser but merely stay on the outer surface of the releaser.
It should be noted that the container is one type of releaser, and the amount of attractant in the container is not directly related to the size of the container, the size of the aperture and the area of the orchard applied. However, in any event, the amount of attractant needs to be sufficient to attract a large population of insects to rest on the outer surface of the container. Since the attractant will be slowly released over time at a rate, the amount of attractant used will vary, taking into account the insect's response to the spatial concentration of the attractant and the actual field age. Some insects are very sensitive to their pheromones and can be attracted at 0.001mg, whereas above this dose they cannot be attracted, and experiments have shown that it is not economical to have too low an amount of attractant so that frequent addition of attractant is required.
According to another aspect of the present invention, there is provided a method of trapping and killing insects, which employs the insect trapping and killing device.
The insect trapping and killing device is placed among crops to be protected according to the number of 1-30 per mu. We found through research that the distance for the peach fruit borers to be attracted by pheromone is 4m, so the suitable distance between the devices is 8m (the effective radius is 4m), and the density is also suitable for the apple leaf rollers, the codling moths and the sweet potato weevils. The data show that the attraction action distance of the pheromone to the chilo suppressalis is 15m, so that the arrangement density of the effective devices is 5 per mu, the density is also suitable for the corn borers, the empoasca vitis and the carina poiretii, and the arrangement of 1 device per mu is suitable for the anoplophora nivea.
to minimize the labor required to control the target insects, the attractants required by the present invention need to be continuously effective over the period of time that the target insect pest is being controlled. Taking fruit moth, longicorn and tea tree hemiptera insects as examples, the action period of the attractant is from the first generation of the target pest imago to the end of the harvest of the target crop. Taking rice-stem borers and corn borers as examples, the action period needs to begin to occur or migrate from the spring of the target insect pest imagoes to the target crops for completing the harvest.
Compared with the situation that the insecticide is directly sprayed to crops, the insect trapping and killing device can effectively control the number of target pests in the field. The device is a novel trapping and killing device, and farmers do not need to use insecticides to control insects. The interference mating technology and the trapping technology are already applied to the prevention and treatment of the harm caused by leaf rollers, codling moths, lesser leafhoppers, corn borers, chilo suppressalis and the like, but the trapping technology used for the pests at present is limited to the utilization of sticky plates or water-containing containers (such as water basins) to kill the trapped target pests, and the attracting cores, the sticky plates and the water basins need to be frequently replaced, so that the effect is short, the labor cost is high, and the effect is very little. The novel device has remarkable insecticidal effect, and is still effective for coleoptera pests, sweet potato weevil, anoplophora chinensis and carina carinata, particularly for pests which are inapplicable to pheromone mating interference technologies such as hemiptera pests, pseudobulbus vitis vinifera and the like. The device eliminates the need of spraying insecticide on orchard crops while trapping and killing insects, so that not only is a good fruit protection effect achieved, but also the safe edibility of fruits is maintained, and a good technical effect can be achieved in the aspect of preventing and treating insect harm.
Drawings
FIG. 1 shows an insect attracting and killing device of an envelope or bag-shaped releaser in accordance with example 1
FIG. 2 shows an insect trapping and killing device of the plate-shaped releaser in example 2
FIG. 3 shows an insect attracting and killing device wound with a tape-like releaser in accordance with example 3
Reference numerals: 1-an attractant; 2-an insecticide; 3, air holes; 4-fixing the nail wound with the tape.
Detailed Description
The present invention will be further described with reference to specific embodiments.
The shape of the releaser in the insect trapping and killing device of the invention is as follows:
Example 1
An insect trapping and killing device comprises an attractant (1), a releaser for carrying the attractant and an insecticide (2) coated on the outer surface of a container, wherein the releaser is in the shape of an envelope or a bag, as shown in figure 1 in particular.
Example 2
An insect trapping and killing device comprises an attractant (1), a releaser for carrying the attractant and an insecticide (2) coated on the outer surface of a container, wherein the releaser is a plate, air holes on the plate are marked with 3, and the attractant is placed between two plastic plates, as shown in a specific figure 2.
Example 3
An insect trapping and killing device comprises an attractant (1), a releaser for carrying the attractant and an insecticide (2) coated on the outer surface of a container, wherein the releaser is a winding belt, and nails for fixing the winding belt are marked with 4, and particularly shown in figure 3.
Example 4
The preparation of the peach fruit borer pheromone attractant comprises the following steps:
Preparation of attractant 1:
Preparation of attractant 2:
The above two attractants are prepared by adding mixture of attractants to butyl rubber head carrier, and absorbing with rubber carrier.
Preparing an apple leaf roller pheromone attractant:
Adding the above attractant mixture into slow release agent containing 2500 mg wax, and injecting into polypropylene small container (diameter 2.5 cm, depth 1.0 cm, upper opening, a container similar to bottle cap, the following steps)
The preparation of the codling moth pheromone attractant comprises the following steps:
Adding the above attractant mixture into slow release agent containing 2500 mg wax, and injecting into polypropylene small container (diameter 2.5 cm, depth 1.0 cm) in molten state
Preparing a rice stem borer pheromone attractant:
Adding the above attractant mixture into slow release agent containing 2500 mg wax, and injecting into polypropylene small container (diameter 2.5 cm, depth 1.0 cm) in molten state
Preparing a corn borer pheromone attractant:
Adding the above attractant mixture into slow release agent containing 2500 mg wax, and injecting into polypropylene small container (diameter 2.5 cm, depth 1.0 cm) in molten state
Preparing the pheromone attractant for the sweet potato weevil:
adding the above attractant mixture into slow release agent containing 2500 mg wax, and injecting into polypropylene small container (diameter 2.5 cm, depth 1.0 cm) in molten state
the preparation of the anoplophora chinensis pheromone attractant comprises the following steps:
Adding the above attractant mixture into slow release agent containing 2500 mg wax, and injecting into polypropylene small container (diameter 2.5 cm, depth 1.0 cm) in molten state
The preparation of anoplophora glabripennis pheromone attractant comprises the following steps:
Adding the above attractant mixture into slow release agent containing 2500 mg wax, and injecting into polypropylene small container (diameter 2.5 cm, depth 1.0 cm) in molten state
Preparing a pheromone attractant for the giant salamander of the tiger:
Adding the above attractant mixture into slow release agent containing 2500 mg wax, and injecting into polypropylene small container (diameter 2.5 cm, depth 1.0 cm) in molten state
Vegetable oils have a relatively high boiling point and are more suitable as solvents for fusing with hot waxes.
preparing a host volatile attractant for the empoasca vitis:
Adding the above attractant mixture into slow release agent containing 3000 mg wax, and injecting into polypropylene small container (diameter 2.5 cm, depth 1.2 cm) in molten state
Preparation of amine-containing attractant for fruit fly
Adopts a small bag made of high-density polyethylene synthetic paper which can be sealed at high temperature
Dimensions 10cm x10cm.
the following 20 grams of mixture were charged:
Ammonium acetate 80% w/w
20% w/w of wood flour
example 5: comparative experiments on the attraction effect of Z, 13 Eicosen-10-one and Z, 7 Tricosene on peach fruit borer:
Compared with the attractant containing the peach fruit moth pheromone component described in the embodiment 4, 15 monitoring boxes are arranged in a 4-mu jujube orchard seriously damaged by the peach fruit moth, sticky plates are arranged in each monitoring box, the layout of the sticky plates is five rows and three columns, and the row spacing and the column spacing are both 10 meters. Wherein 10 of the above materials are filled with Grapholitha molesta pheromone attractant, and 5 are blank controls
Test 1 contained 1, 5 replicates of the attractant described in example 4
Test 2 pheromone attractant releaser containing both attractant 1 and attractant 2, 5 replicates
Run 3 control-no attractant, 5 replicates
The traps were observed once a week, data collected, and 4 weeks of continuous observation
table 1 pheromone trap peach fruit borer control results (total peach fruit borer in 5 traps)
Number of days Test 1 Test 2 Test 3
0-7 9 35 0
8-14 8 28 0
15-21 14 27 0
21-28 6 41 1
Total of 37 131 1
The attracting effect of the pheromone-containing attractant release device (test 2) was superior to the attracting effect of the pheromone-containing attractant alone (test 1).
Example 6: control of pheromone attractant slow-release carrier of different peach fruit borers for attracting effect of peach fruit borers in field
The best type was found by comparing 3 types of the peach fruit borer pheromone attractant releaser material. The pheromone attractant was placed in a monitoring box with a sticky plate. 15 monitoring boxes (5 rows, 3 traps in each row) are hung in a 5-mu jujube garden, 10 meters are arranged between every two monitoring boxes at intervals, the monitoring boxes are observed once a week, data are collected, and the monitoring boxes are continuously observed for 20 weeks.
Test 1: one each of the peach fruit borer pheromone attractants 1 and 2 of the butyl rubber material as described in example 4;
Test 2: a rubber lure having 100 mg of pentane, 1mg of Z, 13 Eicosen-10-one, 4 mg of Z, 7 Tricosene;
Test 3: square black polyethylene foam pieces 2cm x2cm x0.5cm in size containing 1000 mg of pentane, 1mg of Z, 13 Eicosen-10-one, and 4 mg of Z, 7 Tricosene.
TABLE 2 control results for different types of attractant releasers
(Total peach borer in 5 traps per experiment)
Number of days Test 1 Test 2 Test 3
0-7 0 0 1
8-14 31 23 16
15-21 32 15 22
21-28 34 12 21
29-35 19 9 14
36-49 28 8 8
56-70 11 3 5
71-84 5 1 4
85-98 28 9 8
99-112 37 12 16
113-126 31 8 6
127-140 41 7 5
Total of 297 84 109
The attracting effect of the attractant of the test 1 is superior to that of the attractants of the tests 2 and 3, and the attracting effect of the attractant of the test 1 on the peach fruit borers starts to appear about one week later and gradually increases within 8-14 days. The effect lasts for more than 5 months.
Example 7: field trials on the effect of releaser external surface area on trap killing effect in devices
This test was conducted to determine the optimum area of the outer surface of the release for contact. We used high density polyethylene synthetic paper to make envelopes of different areas. The attractant is placed in the envelope bag, and the adhesive is sprayed on both sides of the outer surface of the envelope bag, so that the target pests attracted by the attractant in the envelope bag can be stuck and die after staying on the surface of the envelope bag.
the method can facilitate the collection of the number of attracted target pests in experiments.
The attractants placed in each envelope bag are two peach fruit borer pheromone attractants described in the above example 4, the two attractants 1 and 2 are placed in the same bag, and 20 envelope bags with the attractants placed in the outer glue are placed in a jujube tree garden of 5 mu, and the arrangement is four rows and five columns, and the spacing and the column spacing are both 10 meters.
TABLE 3 field test results of the effect of the external surface area of the load on the trapping effect
(Total amount of peach fruit borers stuck to the outer surface of 4 envelope bags per experiment)
Number of days Test 1 test 2 Test 3 test 4 Test 5
0-7 9 22 24 17 14
8-14 11 21 25 22 18
15-21 8 19 30 22 25
22-28 5 15 18 25 28
Total of 33 77 97 86 85
All traps were effective, with test 1 being the least effective, indicating too little contact surface area. Test 2 has a smaller number of heartworm arrested than tests 3, 4, 5, but this difference is not statistically significant, so it is concluded that the optimum surface area for the selected touchdown region is between 90 and 1536 square centimeters.
Example 8: control tests of different insecticides on the killing effect of peach fruit borers in a laboratory environment, a series of pyrethroid pesticides were tested to determine whether they are suitable for killing peach fruit borers.
A polypropylene bottle (diameter 2.5 cm, length 10 cm) with a volume of 50 ml is used, 2ml of acetone containing different pesticide raw medicines is added, the active ingredients of the pesticide raw medicines contained in the acetone are fully dissolved, the mixture is kept still for one day until all the solvent containing the acetone is volatilized, and different types of pesticides with different concentrations are attached to the inner surface of the bottle. A polypropylene bottle containing no insecticide and only 2ml acetone was also prepared as a control.
The 5 laboratory cultured peach fruit borers were placed in bottles that had been allowed to stand for 10 minutes, then transferred to empty PVC water cups, sealed with wet cotton cloth and rubber bands, allowed to air circulate, and observed after 24 hours. Each experiment was repeated 5 times.
TABLE 4 mortality of peach fruit borer 24 hours after 10 minutes exposure to different pyrethroid insecticides, respectively
mg/square centimeter 0.25 0.50 2.50 5.00
Deltamethrin 70% 90% 100% 100%
Efficient cyhalothrin 80% 100% 100% 100%
Cypermethrin 50% 70% 90% 100%
fenvalerate 30% 60% 80% 100%
Biphenthrin 50% 80% 90% 100%
Controlled variable 0.0% 10.0% 0.0% 0.0%
Tests show that deltamethrin and lambda-cyhalothrin have the best effect on killing peach fruit borers.
Example 9: testing field pot life of insecticide attached to releasers of different materials in a device
In the devices of the present invention, the pesticide applied to the outer surface of the releaser is maintained in the field for an effective period of time which is critical to the effectiveness of such devices. Thus, we tested a series of different releaser materials to determine if the field useful life of the insecticide would therefore vary. The test materials were as follows:
High density polyethylene synthetic paper (spin-bonded polyethylene), polypropylene fiber (nonwoven), PVC board (0.5 mm), polyethylene/starch board (0.5 mm), cotton fabric (cloth) and pla (polylactic acid) polylactic acid nonwoven. All materials were one size (10cm x12 cm).
5 ml of deltamethrin solution or beta-cyfluthrin ethanol solution (0.12 mg/ml) is sprayed on both sides of all the materials, 5 micrograms of insecticide are attached to the surface of each square centimeter of the materials after air drying, and the test is repeated three times.
The releasers are respectively hung on the branches of the peach trees, and the releasers are hung in spring and the test is finished after summer.
and respectively cutting a small piece from each piece of material to return to the laboratory at the time points of 2, 4 and 6 months in the suspension outdoor, and putting 10 peach fruit borers and moths into a culture dish for each time aiming at each piece of cut material, so that the peach fruit borers and moths stay on the surface of the test material for 10 minutes. Thereafter, the test piece was transferred to a paper cup and the mortality was observed 24 hours later. The control group had no material containing the same insecticide hung outdoors.
TABLE 5 mortality of peach fruit borers in outdoor efficacy time test with different insecticides attached to the releaser materials
The outdoor persistence of the high-efficiency cyhalothrin is superior to that of deltamethrin; the effects of the cotton, the high-density polyethylene synthetic paper and the polylactic acid non-woven fabric PP material are better.
Example 10: prevent and treat peach fruit borer field device
The field experiment for preventing and controlling peach fruit borers through the device is carried out in the Yunnan green jujube garden, and the device effects of three kinds of use densities are tested. A 12 x10cm envelope coated on its outer surface with 5 micrograms deltamethrin per square cm was used. In late April, the peach fruit borer adults were hung on branches approximately 1.8 meters high before they emerged. The envelope bag made of the peach fruit borer non-woven fabric PP material contains the attractant 1 and the attractant 2 which are described in the embodiment 4.
And randomly selecting 100 fruits on 5 trees in the center of the test area to judge the infection condition, and if obvious wormholes appear, judging that the trees are damaged.
TABLE 6 sum of percentage of damaged fruits in green jujube orchard harvesting period
From the above experiments, it can be seen that the harm of the orchard using the peach fruit borer control device is significantly lower than that of the blank control, and in the orchards using 10 and 30 envelope bags per mu, the harm of the peach fruit borer is significantly lower than that of the conventional control orchards using full pesticide spraying.
Example 11: field test using attractant device for preventing and controlling apple leaf rollers and peach fruit borers simultaneously
The field experiment of using the device to simultaneously control the apple leaf rollers and the peach fruit borers is carried out in the Liaoning apple orchard, and the effects of the trapping and killing devices with three densities are compared. A 12 x10cm Tyvek envelope was used with 5 micrograms deltamethrin per square cm of the surface of the bag. In late April, the apple leafroller moth and peach fruit borer are hung on a branch about 1.8 meters above the ground before the adult apple leafroller moth and peach fruit borer occur. The attractants in the envelope bag are a peach fruit borer pheromone attractant (attractant 1 and attractant 2 described in example 4) and a codling moth pheromone attractant (codling moth pheromone attractant described in example 4).
Each envelope bag contains pheromone attractant releaser for apple leaf roller moth and peach fruit borer
And randomly selecting 100 fruits on 5 trees in the center of the test area to judge the damage condition, recording the number of the fruits damaged by the apple leaf roller and the peach fruit borer, and calculating the damage rate.
TABLE 7 statistics of fruit damage by apple leafroller and peach fruit borer at harvest time of apple orchard
The effect of controlling the apple leaf rollers and peach fruit borers in orchards with 5, 10 and 20 devices per mu is superior to that of blank areas without treatment and chemical control areas with traditional pesticide spraying. The experiment shows that different pheromone attractants can be used by the same releaser aiming at different target pests at the same time to form a device which saves cost more effectively, the limitation that one biocontrol means can only aim at one target pest in the past is changed, and the two insect attractants have synergistic action.
Example 12: field trials using a device with a codling moth attractant releaser
aiming at codling moth, the experiment of using the device of the codling moth attractant releaser in the fields is carried out in Gansu apple orchard, and the effects of the devices with three densities are compared. A 12 x10cm Tyvek envelope was used with 5 micrograms deltamethrin per square cm of the surface of the bag. In late April, the codling moth was hung at a branch 1.8 meters high above the ground before it appeared. The codling moth pheromone attractant releaser is described in example 4. Tyvek (Tyvek) is an excellent nonwoven product from dupont, a spunbond olefin made with high density polyethylene fibers that possess balanced physical properties.
Each envelope bag contains the codling moth pheromone attractant
Randomly selecting 100 fruits on 5 trees in the center of the test area to judge the infection condition, recording the number of the fruits damaged by the codling moth, and calculating the damage rate.
TABLE 8 fruit hazard rate to codling moth at harvest stage in apple orchard
The use of 5, 10, 20 device systems per acre outperformed the untreated control and orchards using traditional pesticide sprays.
the device provides an effective control method for controlling the plutella xylostella of the subfamily Plutella xylostella. We can therefore reasonably conclude that moths of other subfamily of tortricidae can also use this device to reduce harm by their respective pheromones.
Example 13: field control experiment using Chilo suppressalis slow-release attractant releaser
Experiments for preventing and controlling chilo suppressalis in the field are carried out in Jiangsu paddy fields, and the device effects of the two densities are compared. A 12 x10cm Tyvek envelope was used with 5 micrograms deltamethrin per square cm of the surface of the bag. The chilo suppressalis is hung on a bamboo stick and inserted into a paddy field in the late March, and the rice chilo suppressalis pheromone attractant is as described in example 4.
500 rice ears endangered by chilo suppressalis were randomly selected from the harvested rice field and observed. Hazard was calculated by building up a formula (1.0% dry center 2.5% harvest loss, 1.0% white ear 4.0% loss, 1.0% dry sheath 6.4% loss)
TABLE 9 losses in the harvest stage of the rice field
Test of Conventional pesticide control 4 devices per mu 8 devices per mu
Loss of crop 31.6% 9.3% 4.8%
Compared with the traditional pesticide spraying method, 4 or 8 devices for preventing and controlling chilo suppressalis per mu are used, the yield of rice is increased.
Example 14: field trials using a releaser device containing sweet potato weevil attractant
The field experiments were conducted in the Guangdong potato area and the effect of the trap-killing device was compared for both densities. A 12 x10cm Tyvek envelope was used with 5 micrograms deltamethrin per square cm of the surface of the bag. Suspended on the bamboo culm above the crop at the beginning of 5 months, sweet potato weevil pheromone attractant as described in example 4.
And (4) randomly extracting 500 sweet potatoes in a harvest period, checking the harmfulness of the sweet potatoes caused by the weevils, recording data, and judging prevention and control results.
TABLE 10 damage of sweet potato by sweet potato weevil in harvest period
Test of Conventional pesticide control 4 devices per mu 8 devices per mu
Hazard rate 21.4% 4.9% 2.7%
Example 15: field experiment using anoplophora chinensis attractant releaser
And performing field experiments in the Yunnan walnut nut planting garden, and comparing the trapping and killing device effects of the two densities. Tyvek material 100 x10cm was wrapped around the trunk, and the surface of the Tyvek material was coated with 5 micrograms per square centimeter of lambda-cyhalothrin. Around the trunk of the test orchard from the beginning of 5 months and fixed with nails, the star longicorn pheromone attractant was as described in example 4. The attractant is fixed on the Tyvek material wound on the trunk by clinging.
And simultaneously, two celestial cow monitors containing celestial cow attracting cores are hung in two test areas and a blank area respectively, and the number of celestial cows attracted in the celestial cow monitors is recorded to judge the effectiveness of the slow release control mechanism of the celestial cows. The period is 1 month.
TABLE 11 number of anoplophora chinensis lured in traps under different treatments
The average of the two monitors' induced anoplophora chinensis, the control effect of 1 and 2 anoplophora chinensis devices per mu is far superior to that of the untreated control area.
Example 16: field experiment using anoplophora glabripennis attractant releaser
The field experiment is carried out on the Jiangsu poplar forest, and the trapping and killing device effects of the two densities are compared. Tyvek material 100 x10cm was wrapped around the trunk, and the surface of the Tyvek material was coated with 5 micrograms per square centimeter of lambda-cyhalothrin. Around the trunk of the test orchard from the beginning of 5 months and fixed with nails, star longicorn pheromone was induced as described in example 4. The attractant is tightly fixed on the Tyvek material wound on the trunk.
And simultaneously, two longicorn monitors containing anoplophora glabripennis luring cores are hung in the two test areas and the blank area respectively, and the number of anoplophora glabripennis luring cores in the longicorn monitors is recorded to judge the effectiveness of the anoplophora glabripennis device.
TABLE 12 number of anoplophora glabripennis induced in the monitor under different treatments
The average of the two monitors, using 1 and 2 anoplophora glabripennis devices containing anoplophora glabripennis attractant releasers per acre, gave far better control than the untreated control.
Example 17: field test using corn borer device containing corn borer attractant
And performing field experiments in the inner Mongolia corn planting area, and comparing the effects of the trapping and killing devices with two densities. A 12 x10cm Tyvek envelope was used with 5 micrograms deltamethrin per square cm of the surface of the bag. The adult worms were suspended on the bamboo culm above the crop before the first week of 6 months, and the corn borer pheromone attractant was as described in example 4.
100 immature ears of corn were randomly drawn to determine the damage.
TABLE 13 crop losses
The tests prove that the slow-release prevention and control mechanism provides a method for effectively preventing and controlling chilo suppressalis and Asiatic corn borer. It can therefore be deduced that the species of the family Cnaphalocrocis can also be effectively controlled by using the pheromone specific to the species and the sustained-release control mechanism of the invention.
Example 18: duration test for fruit fly attractant bags
The experiment was carried out in Fujian balsam pear field, 5 bags containing fruit fly attractant were repeatedly placed in the trapping barrel three by three each, and the number of fruit flies in the barrel was observed every week
The 5-type fruit fly attractant respectively uses carrier wood flour, vermiculite, perlite, silica gel and clay, and the raw materials have the same function as the wood flour. The weight ratio of the ammonium acetate of the fruit fly attractant to the carrier is 80: 20.
Table 14 is data for melon flies that were lured using amine-containing fly attractant bags.
The following tests demonstrate that the efficacy of the amine-containing fruit fly attractant bags is over 3 months.
Table 14 data of melon flies trapped by the luring agent bag for trapping the melon flies in the barrel
Example 19: prevention and control test for citrus orchard by using citrus fruit fly trapping and killing device
And performing field experiments in the Fujian orange area, and comparing the effects of the trapping and killing devices with two densities. A 12 x10cm Tyvek envelope was used with 5 micrograms deltamethrin per square cm of surface of the bag. The envelope bag filled with the fruit fly attractant is hung on a branch about 1.8 meters above the ground. The fruit fly attractant was as described in example 4.
At harvest, 300 fruits were randomly picked to record the number of fruits damaged by Bactrocera dorsalis
TABLE 15 fruit ratio endangered by Bactrocera dorsalis
test of Conventional pesticide control Using 4 devices per mu Using 8 devices per mu
Rate of damage to fruit 9.1% 2.1% 1.1%
As can be seen from the test results, the control effect of using 3-6 fruit fly control devices per mu is superior to that of the conventional pesticide control.
Example 20: experiment using tea garden artificial eye green leafhopper prevention and control device
And performing field experiments in the Fujian tea garden, and comparing the effects of the trapping and killing devices with two densities. A 12 x10cm Tyvek envelope was used and the attractant was as described in example 4, with 5 micrograms deltamethrin per square cm of surface of the bag. Hanging it on a bamboo pole higher than the tea crown. 3 green adhesive plates are respectively placed in each mu of the test area and the blank control area to trap tea lesser leafhoppers.
The occurrence condition of the tea lesser leafhoppers at each test point and each blank control point is judged by observing the quantity of the tea lesser leafhoppers on the green sticky boards at the test area and the control area
TABLE 16 comparison of the number of tea leafhoppers on the sticky board at each test point in the tea garden
According to the quantity of the tea lesser leafhoppers collected on the green sticky boards in different test areas, the conclusion can be drawn that the control effect of the tea lesser leafhopper prevention and control device of 5 or 10 tea lesser leafhoppers used per mu is superior to that of the conventional pesticide spraying.
Example 21: test on control mechanism of coffee deinsectization carillon
The field experiment was performed in the Yunnan Arabica coffee growing area and the effect of the trapping and killing device was compared for both densities. A 12 x10cm Tyvek envelope was used with 5 micrograms per square cm of cyfluthrin coated on the surface of the bag. The tree fork is hung on the tree fork in the beginning of the fifth month. The coffee deinsectization boneset pheromone attractant described in the examples was used and placed in a Tyvek envelope bag. Two sticky plates and a coffee deinsectization longicorn monitoring lure are hung in the central area of each test area, the number of the caught coffee deinsectization longicorn is collected and monitored, and comparison is carried out.
Monitoring the number of coffee deinsectization carinii on 2 sticky plates hung at the center of each test area every week, recording data, continuously checking for 6 weeks, and comparing prevention and effect according to the number of the collected coffee deinsectization carinii.
Table 17 records the number of coffee deinsectization carinii collected on each test stripping plate
Examples 15, 16, 21 provide control methods to control three species of carica carinata. From these test results, it can be concluded that other species of carica such as longicorn, longicorn and other important longicorn species can be controlled by the device using their respective pheromones.
Although the present invention and its advantages have been described in detail with reference to specific embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims. Moreover, the scope of the present application is not intended to be limited to the particular embodiments of the apparatus, methods, and steps described in the specification. As one of ordinary skill in the art will readily appreciate from the disclosure of the present invention, devices, methods, and steps, presently existing or later to be developed that perform substantially the same function or achieve substantially the same result as the corresponding embodiments described herein may be utilized according to the present invention. Accordingly, the appended claims are intended to include within their scope such devices, methods, and steps.

Claims (5)

1. an insect trapping and killing device, comprising:
a. An attractant; b. a releaser for carrying an attractant; c. an insecticide coated on the outer surface of the releaser; wherein the attractant comprises: insect pheromones added with antioxidant, anti-ultraviolet stabilizer and solvent; a plant volatile attractant with an antioxidant and an anti-ultraviolet stabilizer; an amine-containing attractant and a slow-release carrier, wherein the weight ratio of the insect pheromone, the antioxidant, the anti-ultraviolet stabilizer, the solvent and the slow-release carrier is (1-10): (1-2): (0.5-1.0): (400-450): (2000-3000); and the weight ratio of the plant volatile attractant to the antioxidant to the ultraviolet-resistant stabilizer to the slow-release carrier is (700- & 800): (50-60): (10-20): (2000-3000); the weight ratio of the amine-containing attractant to the slow release carrier is (20-80): (80-20), wherein the insect pheromone is selected from one or more of peach fruit borer, codling moth, chilo suppressalis, Asian corn borer, sweet potato weevil, longicorn adult or coffee beetle longicorn pheromone; the plant volatile attractant is selected from plant fragrances that are attractive to empoasca vitis; the amine-containing attractant is ammonium acetate, and the slow release carrier is selected from one or more of vermiculite, perlite, silica gel, clay and wood powder; the releaser is selected from high-density polyethylene synthetic paper, polypropylene fiber, PVC board, polyethylene/starch board, cotton fabric or PLA non-woven fabric bag.
2. An insect trapping and killing device according to claim 1, wherein said release means is selected from the group consisting of a plate, a container, a bag, and a device that can be wrapped around the trunk and branches of a tree.
3. An insect trapping and killing device according to claim 1, wherein the releaser has an outer surface area of 24-1536 cm.
4. The device as claimed in claim 1, wherein the insecticide is a pyrethroid insecticide selected from the group consisting of deltamethrin, alpha-cypermethrin, beta-cypermethrin, bifenthrin, fenvalerate and mixtures thereof.
5. A method for trapping and killing insects, characterized in that the insect trapping and killing device of any one of claims 1-4 is used, and the device is placed between crops to be protected according to the quantity of 1-30 insects per mu.
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CN108450465B (en) * 2018-03-23 2020-10-09 南京新安中绿生物科技有限公司 Volatile attractant for bactrocera dorsalis and application thereof
CN108496968B (en) * 2018-03-28 2020-05-26 福建省农业科学院植物保护研究所 Sweet potato weevil attractant
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