CN104938486A - Diaphania perspectalis sex attractant and lure core and preparation method thereof - Google Patents
Diaphania perspectalis sex attractant and lure core and preparation method thereof Download PDFInfo
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Abstract
The invention relates to an insect sex attractant, and in particular relates to a diaphania perspectalis sex attractant and a lure core and a preparation method thereof. The effective component of the diaphania perspectalis sex attractant comprises the following components in parts by weight: 1-50 parts of cis-11-hexedecanal and 1-20 parts of trans-11-hexedecanal, wherein the lure core of the diaphania perspectalis sex attractant comprises a sex attractant, a solvent and a lure core carrier; the sex attractant is the attractant; the solvent is any one of normal hexane, dichloromethane, diethyl ether or acetone; and the lure core carrier is a compounded rubber lure core carrier or a polyethylene plastic pipe. The technical scheme provided by the invention is optimized by a plurality of creative screening experiments of components and proportions of the sex attractant and combination with the characteristics of the geographic area; and the sex attractant and the lure core capable of efficiently preventing and treating diaphania perspectalis and the preparation method of the sex attractant and the lure core are obtained.
Description
Technical field
The present invention relates to insects attractant, be specifically related to a kind of Diaphania perspectalis sex attractant and lure core and preparation method thereof.
Background technology
Diaphania perspectalis (Diaphania perspectalis or Glyphodes perspectalis) (Walker, 1859) be Lepidoptera Pyralidae insect, mainly be distributed in the Asian countries such as Japan, Korea S, China, India at first, after importing Germany into first from 2006, spread multiple country to Europe, and have the gesture further expanded.Due to multiple ornamental plants of Buxaceae such as larval feeding Chinese littleleaf box, Buxus bodinieri, gold leaf Chinese littleleaf boxes, often blade is eaten up during outburst, cause plant withered, have a strong impact on urban look.For fullying understand the characteristic of this insect and carrying out effectively preventing, researchers have carried out studying comparatively widely to many-sides such as its occurring rhythm, biological property, artificial feeding and geographical distributions thereof.In existing technology, most employing insecticide carries out the control of Diaphania perspectalis, but its human cost consumption of use of insecticide is large, and the control for the Diaphania perspectalis of large area ornamental plant is very not convenient, effectively can not realize the control of Diaphania perspectalis damage by disease and insect.
At present for the control of ornamental plant damage by disease and insect, insect sex attractant product is more aobvious important for the control of damage by disease and insect, insect sex attractant product is bionical high-tech product, by the sex pheromone compound luring core to discharge Prof. Du Yucang, and slowly-releasing is to field, lures male worm to trapper, and kill male worm with Physical, thus destroy its mating, finally reach the object of control.Carry out control of insect by trapper, have and lure worm single-minded, do not bring out pest resistance, preserve the ecological environment and beneficial organism population.Usability pheromones carries out the control of damage by disease and insect, can overcome that gardens distribution area is large, landform and the changeable and limitation that causes of physical features.The method can carry out biological control or chemical control to insect effectively, thus by the initial stage that injurious insect control is occurring, to reduce the loss to greatest extent.Simultaneity pheromones can be used as monitoring, the prediction of disease insect, has in time, accurately understands generation and the growth and decline situation of woodland disease insect.But because garden shrub species is different from crops, it is distributed in park, urban road and garden etc., and tradition utilizes insecticide control more difficult.Therefore, usability pheromones trapping adult is to reduce male and female mating, and the ovum causing female moth to give birth to fails to hatch, and then the field quantity of controlled Diaphania perspectalis larva processed is at reduced levels, thus effectively can reach the object of control.
But the invention for Diaphania perspectalis sex pheromone in existing technology is less, and due to long-term geographic isolation, insect evolution of the same race defines the Palaeobiogeography difference of sex pheromone, the difference of the present female moth sex pheromone secretion composition of this difference table and the difference of male moth on sensory reaction.Insect sex pheromone Palaeobiogeography difference shows as two types, and a class is that different geographic populations has identical sex pheromone composition, but the concentration ratio difference between each component, another kind of, be that the chemical constitution of each component is not identical.In the insect sex pheromone identified, majority of case is that the composition of sex pheromone and ratio thereof there are differences; And there is variation kind for Diaphania perspectalis insect, existing Diaphania perspectalis sex pheromone can not realize effectively control to the Diaphania perspectalis of mutational variety.Kind of a harmful insect often occurs cause the variation of harmful property to farm-forestry crop thus cause insect population outbreak in Diaphania perspectalis variation.Also can cause the changes such as its behavior, physiological characteristic, the history of life, as grown, taking food, survive, the variation etc. of mating and egg production capacity.Larva and the adult life power of melanism individuality are usually strong than normal type, and growing with mating behavior, also there were significant differences, greatly affect its breeding thus.
As can be seen here, can invent a kind of based on the Diaphania perspectalis sex pheromone of China geographic area and effective proportioning of each composition, obtain a kind of can high-efficiency prevention and control Diaphania perspectalis sex attractant and lure core and preparation method thereof to become those skilled in the art's technical problem urgently to be resolved hurrily.
Summary of the invention
The present invention is in order to solve the problems of the technologies described above, a kind of Diaphania perspectalis sex attractant based on China geographic area is provided and lures core and preparation method thereof, the present invention is by the creative screening experiment of a large amount of sex attractant composition and proportioning and in conjunction with the feature of geographic area, optimize technical scheme of the present invention, obtain a kind of sex attractant of high-efficiency prevention and control Diaphania perspectalis and lure core and preparation method thereof.
In order to reach above-mentioned technique effect, technical scheme of the present invention comprises:
A kind of Diaphania perspectalis sex attractant, the active ingredient of described Diaphania perspectalis sex attractant comprises the component of following parts by weight:
Along 11-hexadecenal 1 ~ 50 part
Anti-11-hexadecenal 1 ~ 20 part.
Preferably, the active ingredient of the attractant of described Diaphania perspectalis comprises the component of following parts by weight:
Along 11-hexadecenal 5 ~ 15 parts
Anti-11-hexadecenal 5 ~ 10 parts.
More preferably, the active ingredient of the attractant of described Diaphania perspectalis comprises the component of following parts by weight:
Along 11-hexadecenal 5 parts
Anti-11-hexadecenal 5 parts.
The active ingredient of described Diaphania perspectalis sex attractant also comprises the component of following parts by weight:
Along 11-hexadecenol 1 ~ 15 part
Along 9-hexadecenal 1 ~ 10 part.
A kind of Diaphania perspectalis sex attractant lures core, described Diaphania perspectalis sex attractant lures core comprise sex attractant, solvent and lure core carrier, described sex attractant is above-mentioned Diaphania perspectalis sex attractant, and described solvent is any one in n-hexane, carrene, ether or acetone; The described core carrier that lures is that compounded rubber lures core carrier or vinyl tube.
Described Diaphania perspectalis sex attractant is dissolved in described solvent, and concentration is 60 ~ 95g/L.
Described Diaphania perspectalis sex attractant lures core also to comprise antioxidant and uvioresistant stabilizing agent.
Described antioxidant is any one in BHA, BHT, PG; Described uvioresistant stabilizing agent be salicylate class, benzophenone class, benzotriazole, group-substituted acrylonitrile, triazines and hindered amines composite in any one.
Preferably, described uvioresistant stabilizing agent is any one in phenyl salicytate, BTA, 2,4-DihydroxyBenzophenone, single benzoic acid resorcinol.
A kind of Diaphania perspectalis sex attractant lures the preparation method of core, each components dissolved of getting Diaphania perspectalis sex attractant by above-mentioned percentage by weight is prepared into sex attractant solution in described solvent, luring in core carrier of every 1ml drips 20 ~ 200 μ g sex attractants, add the antioxidant of 5 ~ 20 μ g and the uvioresistant stabilizing agent of 10 ~ 35 μ g, the Diaphania perspectalis sex attractant be prepared into lures core simultaneously.
Beneficial effect of the present invention comprises:
1, a kind of Diaphania perspectalis sex attractant of the present invention and lure core and preparation method thereof, for China's geographic area feature, test based on a large amount of gardens, optimize a kind of Diaphania perspectalis sex attractant composition of the present invention and each composition proportion, and by rationally screening, obtain a kind of Diaphania perspectalis sex attractant of the present invention and lure core and preparation method thereof, Diaphania perspectalis sex attractant of the present invention and lure core can high-efficiency prevention and control Diaphania perspectalis.Simultaneously Diaphania perspectalis sex attractant of the present invention and lure core to can be used as monitoring, the prediction of Diaphania perspectalis disease insect, has in time, accurately understands the situation of the generation of woodland Diaphania perspectalis, growth and decline variation.
2, a kind of Diaphania perspectalis sex attractant of the present invention and lure core can be used for a large amount of trapping, prediction Diaphania perspectalis, there is very high biologically active, trap and kill in a large number in the forest land being not suitable for use chemical pesticide, reduce the heavy mouth density of woodland, reach the object of control Diaphania perspectalis, and core can be lured to carry out industrialization production by attractant of the present invention, it has good economic benefit, social benefit and ecological benefits.
Accompanying drawing explanation
Figure 1 shows that the EAG response diagram of Diaphania perspectalis male imago of the present invention to variable concentrations dosage sex pheromone and isomer thereof.
Figure 2 shows that the present invention along the single of 11-hexadecenal and anti-11-hexadecenal and combination attractant to the gathering effect figure of Diaphania perspectalis male imago.
Embodiment
Hereafter will describe the specific embodiment of the invention in detail in conjunction with concrete accompanying drawing.It should be noted that the combination of technical characteristic or the technical characteristic described in following embodiment should not be considered to isolated, they can mutually be combined thus be reached better technique effect.
Embodiment 1
A kind of Diaphania perspectalis sex attractant, the active ingredient of described Diaphania perspectalis sex attractant comprises the component of following parts by weight:
Along 11-hexadecenal 1 part
Anti-11-hexadecenal 20 parts.
A kind of Diaphania perspectalis sex attractant lures core, described Diaphania perspectalis sex attractant lures core comprise sex attractant, solvent and lure core carrier, described sex attractant is above-mentioned Diaphania perspectalis sex attractant, and described solvent is any one in n-hexane, carrene, ether or acetone; The described core carrier that lures is that compounded rubber lures core carrier or vinyl tube.
A kind of Diaphania perspectalis sex attractant of preparing lures the preparation method of core: each components dissolved of getting Diaphania perspectalis sex attractant by above-mentioned percentage by weight is prepared into sex attractant solution in described solvent, concentration is 95g/L, luring in core carrier of every 1ml is added dropwise to 20 μ g sex attractants, add the BHA of 5 μ g and the BTA of 10 μ g, the Diaphania perspectalis sex attractant be prepared into lures core simultaneously.
Embodiment 2
A kind of Diaphania perspectalis sex attractant, the active ingredient of described Diaphania perspectalis sex attractant comprises the component of following parts by weight:
Along 11-hexadecenal 50 parts
Anti-11-hexadecenal 1 part.
A kind of Diaphania perspectalis sex attractant lures core, described Diaphania perspectalis sex attractant lures core comprise sex attractant, solvent and lure core carrier, described sex attractant is above-mentioned Diaphania perspectalis sex attractant, and described solvent is any one in n-hexane, carrene, ether or acetone; The described core carrier that lures is that compounded rubber lures core carrier or vinyl tube.
A kind of Diaphania perspectalis sex attractant of preparing lures the preparation method of core: each components dissolved of getting Diaphania perspectalis sex attractant by above-mentioned percentage by weight is prepared into sex attractant solution in described solvent, concentration is 60g/L, luring in core carrier of every 1ml is added dropwise to 200 μ g sex attractants, add the antioxidant PG of 20 μ g and 2 of 35 μ g simultaneously, 4-dihydroxy benaophenonel, the Diaphania perspectalis sex attractant be prepared into lures core.
Embodiment 3
A kind of Diaphania perspectalis sex attractant, the active ingredient of described Diaphania perspectalis sex attractant comprises the component of following parts by weight:
Along 11-hexadecenal 15 parts
Anti-11-hexadecenal 5 parts.
A kind of Diaphania perspectalis sex attractant lures core, described Diaphania perspectalis sex attractant lures core comprise sex attractant, solvent and lure core carrier, described sex attractant is above-mentioned Diaphania perspectalis sex attractant, and described solvent is any one in n-hexane, carrene, ether or acetone; The described core carrier that lures is that compounded rubber lures core carrier or vinyl tube.
A kind of Diaphania perspectalis sex attractant of preparing lures the preparation method of core: each components dissolved of getting Diaphania perspectalis sex attractant by above-mentioned percentage by weight is prepared into sex attractant solution in described solvent, concentration is 80g/L, luring in core carrier of every 1ml is added dropwise to 25 μ g sex attractants, add the antioxidant BHT of 5 μ g and the phenyl salicytate of 15 μ g, the Diaphania perspectalis sex attractant be prepared into lures core simultaneously.
Embodiment 4
A kind of Diaphania perspectalis sex attractant, the active ingredient of described Diaphania perspectalis sex attractant comprises the component of following parts by weight:
Along 11-hexadecenal 5 parts
Anti-11-hexadecenal 10 parts.
A kind of Diaphania perspectalis sex attractant lures core, described Diaphania perspectalis sex attractant lures core comprise sex attractant, solvent and lure core carrier, described sex attractant is above-mentioned Diaphania perspectalis sex attractant, and described solvent is any one in n-hexane, carrene, ether or acetone; The described core carrier that lures is that compounded rubber lures core carrier or vinyl tube.
A kind of Diaphania perspectalis sex attractant of preparing lures the preparation method of core: each components dissolved of getting Diaphania perspectalis sex attractant by above-mentioned percentage by weight is prepared into sex attractant solution in described solvent, concentration is 75g/L, luring in core carrier of every 1ml is added dropwise to 150 μ g sex attractants, add the antioxidant BHT of 5 μ g and single benzoic acid resorcinol of 20 μ g, the Diaphania perspectalis sex attractant be prepared into lures core simultaneously.
Embodiment 5
A kind of Diaphania perspectalis sex attractant, the active ingredient of described Diaphania perspectalis sex attractant comprises the component of following parts by weight:
A kind of Diaphania perspectalis sex attractant lures core, described Diaphania perspectalis sex attractant lures core to comprise sex attractant, solvent, lures core carrier, antioxidant and uvioresistant stabilizing agent, described sex attractant is above-mentioned Diaphania perspectalis sex attractant, and described solvent is any one in n-hexane, carrene, ether or acetone; The described core carrier that lures is that compounded rubber lures core carrier or vinyl tube.
A kind of Diaphania perspectalis sex attractant of preparing lures the method for core: each components dissolved of getting Diaphania perspectalis sex attractant by above-mentioned percentage by weight is prepared into sex attractant solution in described solvent, concentration is 95g/L, luring in core carrier of every 1ml is added dropwise to 20 μ g sex attractants, add the antioxidant PG of 5 μ g and the BTA of 10 μ g, the Diaphania perspectalis sex attractant be prepared into lures core simultaneously.
Embodiment 6
A kind of Diaphania perspectalis sex attractant, the active ingredient of described Diaphania perspectalis sex attractant comprises the component of following parts by weight:
A kind of Diaphania perspectalis sex attractant lures core, described Diaphania perspectalis sex attractant lures core to comprise sex attractant, solvent, lures core carrier, antioxidant and uvioresistant stabilizing agent, described sex attractant is above-mentioned Diaphania perspectalis sex attractant, and described solvent is any one in n-hexane, carrene, ether or acetone; The described core carrier that lures is that compounded rubber lures core carrier or vinyl tube.
A kind of Diaphania perspectalis sex attractant of preparing lures the method for core: each components dissolved of getting Diaphania perspectalis sex attractant by above-mentioned percentage by weight is prepared into sex attractant solution in described solvent, concentration is 60g/L, luring in core carrier of every 1ml is added dropwise to 200 μ g sex attractants, add the antioxidant BHA of 20 μ g and 2 of 35 μ g simultaneously, 4-dihydroxy benaophenonel, the Diaphania perspectalis sex attractant be prepared into lures core.
Embodiment 7
A kind of Diaphania perspectalis sex attractant, the active ingredient of described Diaphania perspectalis sex attractant comprises the component of following parts by weight:
A kind of Diaphania perspectalis sex attractant lures core, described Diaphania perspectalis sex attractant lures core to comprise sex attractant, solvent, lures core carrier, antioxidant and uvioresistant stabilizing agent, described sex attractant is above-mentioned Diaphania perspectalis sex attractant, and described solvent is n-hexane; The described core carrier that lures is that compounded rubber lures core carrier.
A kind of Diaphania perspectalis sex attractant of preparing lures the method for core: each components dissolved of getting Diaphania perspectalis sex attractant by above-mentioned percentage by weight is prepared into sex attractant solution in described solvent, concentration is 80g/L, luring in core carrier of every 1ml is added dropwise to 100 μ g sex attractants, add the BHT of 10 μ g and the phenyl salicytate of 15 μ g, the Diaphania perspectalis sex attractant be prepared into lures core simultaneously.
Embodiment 8 Diaphania perspectalis lures collection experiment to the tentaculum electric potential test of sex attractant compound and field
1, for examination worm source
Chinese littleleaf box thin,tough silk snout moth's larva larva is collected in Liu Jin garden, Haishu District, Ningbo City in April, 2013 and is used as on the Buxus sinica of hedgerow.Pupate after laboratory rearing with Buxus sinica blade, sprout wings, after sprouting wings, adult is used for electrophysiologic testing and sex pheromone extraction and analysis, indoor illumination time 14: 10 (L: D), temperature (27 ± 1) DEG C, humidity (70 ± 10) %.Adult is raised with the nutrition as a supplement of 10% G/W.Because Mating status, emergence age etc. all can have influence on the EAG reaction of female worm, namely sex pheromone can affect the sensory receptors of insect feeler, for avoiding the interference of these factors, select the male imago of the non-mating of sprouting wings the same day for test, through anatomic observation, now the wild female worm sexual gland of Chinese littleleaf box thin,tough silk is ripe.
2, for examination chemical reagent
Anti-11-hexadecenal (E11-16:Ald), anti-11-hexadecene acetic acid esters (E11-16:AC), suitable 9-hexadecenal (Z9-16:Ald), suitable 11-hexadecenol (Z11-16:OH), suitable 11-hexadecenal (Z11-16:A1d), suitable 11-hexadecene acetic acid esters (Z11-16:AC) are provided by Ningbo NewCon Co., Ltd., gas chromatographic detection purity >97%.In order to ensure the accuracy of test, control penetrating odor release homogeneous, in test, as retarder thinner, sample is made into 10 using paraffin oil (analyzing pure)
-1molL
-1, 10
-2molL
-1, 10
-3molL
-1, 10
-41molL
-1, 10
-5molL
-1, 10
-6molL
-1.Test electricity consumption physiological saline adopts Kaissling solution, and its formula is: glucose (354mmolL
-1), calcium chloride (1mmolL
-1), potassium dihydrogen phosphate (20mmolL
-1), potassium chloride (6.4mmolL
-1), magnesium chloride (12mmolL
-1), sodium chloride (12mmolL
-1) and potassium hydroxide (9.6mmolL
-1), adjust ph to 6.5.Agents useful for same is Zhejiang culminant star chemical reagent factory product, is analysis pure.
3, the mensuration of tentaculum electric potential
CO is used before mensuration
2diaphania perspectalis is anaesthetized, with knife blade by for subsequent use behind Diaphania perspectalis antenna ablation top.Reference electrode inserts the feeler base portion of Diaphania perspectalis, and recording electrode is enclosed within feeler top.Glass electrode capillary inner diameter 2mm, and pass into the silver-silver chloride glass electrode that diameter is 0.4mm, pour into appropriate Kaissling electro physiology solution in electrode, after tentaculum electric potential record is amplified by Syntech preamplifier, link Syntech IDAC-2, finally input calculator.Test adopts Syntech EAG register system software records, analyzes the change of its current potential.The dissolution homogeneity that in test, each suction 30 μ l prepare drips on the filter paper of 6cm × 0.5cm, and moves in dropper, and dropper end connects micro-tapping control device, and top is inserted to be had in the Pasteur pipe aperture of continuous flow.Flow through clean, the moistening continuous flow of activated carbon treatment is 1200mlmin
-1, stimulation air flow rate is 40mlmin
-1, aiming at Diaphania perspectalis feeler stimulates, and each stimulation time is 0.1s.In whole mensuration process, because the reaction of feeler can weaken, the response value of each compound is adopted n-compound along 3-hexenyl acetate 10
-2.The measured value of each compound of relative value of EAG value divided by the mean value of twice measured value before and after n-compound, then is multiplied by 100% and represents.Measure before and after stimulus compound and stimulate once with n-compound respectively.In order to the peripheral nervous and nervous centralis that prevent insect feeler produce adaptability fatigue, each stimulus intervals at least 30s.Each test replication 6 times.
4, field trapping experiments
Hang on being inserted with the wild trapper of the Chinese littleleaf box thin,tough silk luring core on the street tree side shoot of liftoff 1.5m with iron wire during input.Because major trunk roads generally have symmetry, arrange that trapper both sides intersect and throw in, the fore-and-aft distance between two trappers is 20m.Each arrangement of sample plot 9 kinds lures core and 1 blank, and often kind is repeated 3 times.Different Diaphania perspectalis lures core to be numbered respectively, and trapper is write exactly really with label.Throw in the worm amount being time recording and luring collection for second day, record 7d continuously.The Diaphania perspectalis worm amount that duration of test haftplatte is traped is more, and in trapper, worm amount is namely unified more than 20 changes all haftplattes, to guarantee the accuracy counted.
5, data processing
Data acquisition mean value ± standard error (SE) represents, SPSS17.0 statistical software is adopted to add up and variance analysis data, EAG reacts the significance difference opposite sex and adopts Duncan ' s multiple range test method (SSR, P < 0.05).
6, results and analysis
The mensuration of EAG reaction have selected along 3-hexenyl acetate as n-compound, and this compound is the common Odors of green leaves of plant, and 10
-2the dosage of ml is also the burst size of plant under natural conditions substantially, and therefore, such can reflect the susceptibility that male moth reacts sex pheromone more substantially.The feeler of the male moth of Diaphania perspectalis shows different olfaction reactions to different compound, some compounds are along with the increase of concentration, the reaction of the male moth of Diaphania perspectalis is in the trend risen gradually, and it the results are shown in Figure the EAG of the Diaphania perspectalis male imago of the present invention shown in 1 to variable concentrations dosage sex pheromone and isomer thereof and reacts.Further analysis shows that Z9-16:Ald, Z11-16:A1d, E11-16:Ald ascendant trend is very fast, and strong reaction, 10
-3molL
-1time relative response value exceed 130%, and be 10 in concentration
-1molL
-1time Z11-16:A1d and E11-16:Ald relative response value all more than 300%, and ascendant trend is obvious.For the reaction of Z11-16:OH, its concentration dose EAG response curve ascendant trend is then mild.Then more weak to the EAG reaction of Z11-16:AC and E11-16:AC, react lower than to the EAG of n-compound along 3-hexenyl acetate, and there is no correlation with concentration dose.Through this experiment sieving, the active ingredient obtaining Diaphania perspectalis sex attractant of the present invention can be the combination of suitable 11-hexadecenal and anti-11-hexadecenal, or along 11-hexadecenal, anti-11-hexadecenal, suitable 11-hexadecenol and the combination along 9-hexadecenal.
Single and the combination attractant of the suitable 11-hexadecenal according to Fig. 2 and anti-11-hexadecenal is to the gathering effect of Diaphania perspectalis male imago, the suitable 11-hexadecenal of 9 kinds of different content proportionings and anti-11-hexadecenal, except anti-11-hexadecenal one-component to Diaphania perspectalis without gathering effect except, other all have good attractant effect.The results of analysis of variance shows, the mixture of suitable 11-hexadecenal and anti-11-hexadecenal different ratio is to the gathering effect there was no significant difference of Diaphania perspectalis, and this illustrates that anti-11-hexadecenal is not crucial for the attractive activity of Diaphania perspectalis sex pheromone.
Embodiment 9 Diaphania perspectalis sex pheromone field trapping test
Test method:
Test plot is arranged on Ningbo City Diaphania perspectalis and 6 city major trunk roads such as serious west part of the ring road, Liu Ting street, garden in university Shou Nan Road, Zhongshan Road West, autumn physical circuit, Kechuang North Road occurs.These both sides, section are mainly to plant with the ground cover plant such as tulbaghia violacea, tawny daylily, and Buxus sinica is as hedgerow, and fragrant camphor tree is as street tree, and form the pattern of Qiao, filling, careless compound, environment is more consistent.Hang on being inserted with the wild trapper of the Chinese littleleaf box thin,tough silk luring core on the street tree side shoot of liftoff 1.5m with iron wire during input.Because major trunk roads generally have symmetry, arrange that trapper both sides intersect and throw in, the fore-and-aft distance between two trappers is 20m.Each arrangement of sample plot 9 kinds lures core and 1 blank, and often kind is repeated 3 times.Different Diaphania perspectalis lures core to be numbered respectively, and trapper is write exactly really with label.Throw in the worm amount being time recording and luring collection for second day, record 7d continuously.The Diaphania perspectalis worm amount that duration of test haftplatte is traped is more, and in trapper, worm amount is namely unified more than 20 changes all haftplattes, to guarantee the accuracy counted.
Data processing:
Data acquisition mean value ± standard error (SE) represents, SPSS17.0 statistical software is adopted to add up and variance analysis data, EAG reacts the significance difference opposite sex and adopts Duncan ' s multiple range test method (SSR, P < 0.05).
Difference lures core to the analysis of Diaphania perspectalis gathering effect:
As shown in table 1 is the suitable 11-hexadecenal of different ratio and anti-11-hexadecenal binary mixture and single known along the field trapping amount of 11-hexadecenal to Diaphania perspectalis, and field trapping test lures core all to lure collection to a large amount of Diaphania perspectalis for the different ratio of Z11-16:Ald and the E11-16:Ald composition of examination.
The suitable 11-hexadecenal of table 1 different ratio and anti-11-hexadecenal binary mixture are to the field trapping amount of Diaphania perspectalis
From table 1 data, collection can be lured to can be 1 ~ 50:1 ~ 20 to Diaphania perspectalis Z/E11-16:Ald ratio, preferably, be 3:1 along 11-hexadecenal and anti-11-hexadecenal ratio.
As shown in table 2 is that the suitable 11-hexadecenal of different ratio, anti-11-hexadecenal, suitable 11-hexadecenol and the suitable field trapping amount of 9-hexadecenal quaternary mixture to Diaphania perspectalis are known, and field trapping test lures core all to lure collection to a large amount of Diaphania perspectalis for the different ratio that the suitable 11-hexadecenal of examination, anti-11-hexadecenal binary mixture, suitable 11-hexadecenol and suitable 9-hexadecenal form.，
The suitable 11-hexadecenal of table 2 different ratio, anti-11-hexadecenal, suitable 11-hexadecenol and suitable 9-hexadecenal quaternary mixture are to the field trapping amount of Diaphania perspectalis
From table 2 data, collection can be lured to can be along 11-hexadecenal to the sex attractant of Diaphania perspectalis: anti-11-hexadecenal: along 11-hexadecenol: can be 1 ~ 50:1 ~ 20:1 ~ 50:1 ~ 20 along 9-hexadecenal ratio, preferably, collection can be lured to be suitable 11-hexadecenal to the sex attractant of Diaphania perspectalis: anti-11-hexadecenal: along 11-hexadecenol: can be 1 ~ 50:1 ~ 20:5 ~ 20:5 ~ 10 along 9-hexadecenal ratio, more preferably, collection can be lured to be suitable 11-hexadecenal to the sex attractant of Diaphania perspectalis: anti-11-hexadecenal: along 11-hexadecenol: can be 15:5:1:1 along 9-hexadecenal ratio.
The core that lures that embodiment 1 ~ 7 is prepared by embodiment 10 carries out field trapping experiments
Select Ningbo City Diaphania perspectalis that 6 city major trunk roads such as serious west part of the ring road, Liu Ting street, garden in university Shou Nan Road, Zhongshan Road West, autumn physical circuit, Kechuang North Road occur.These both sides, section are mainly to plant with the ground cover plant such as tulbaghia violacea, tawny daylily, and Buxus sinica is as hedgerow, and fragrant camphor tree is as street tree, and form the pattern of Qiao, filling, careless compound, environment is more consistent.The core that lures prepared by embodiment 1 ~ 7 is volatilized completely until solvent, core will be lured to be fixed on 7 400cm
2insect-sticking plate catch and lure device central authorities, the quantity of Diaphania perspectalis record that arrive are traped in investigation every day, and continue investigation 30 days, result of the test is in table 1.
The core that lures prepared by table 3 embodiment 1 ~ 7 carries out field trapping experiments result
From table 3 data result, core is lured prepared by embodiments of the invention 1 ~ 7, all can trap Diaphania perspectalis, the optimum for preparation of embodiment 3 and embodiment 7 lures core, and its Diaphania perspectalis total amount is maximum, it can thus be appreciated that, a kind of Diaphania perspectalis sex attractant of the present invention and lure core and preparation method thereof can carry out prediction and trapping to Diaphania perspectalis, observes and predicts accurately, and trapping is active high, reach the effect of administering normal type Diaphania perspectalis, suit large area to popularize use.
Above-mentioned detailed description is the illustrating of possible embodiments for invention, and this embodiment is also not used to limit the scope of the claims of the present invention, does not allly depart from equivalence of the present invention and implements or change, and all should be contained in the scope of the claims of the present invention.
In addition, those skilled in the art also can make various amendments in other form and details, interpolation and replacement in the claims in the present invention scope of disclosure and spirit.Certainly, the changes such as these various amendments made according to the present invention's spirit, interpolation and replacement, all should be included within the present invention's scope required for protection.
Claims (9)
1. a Diaphania perspectalis sex attractant, is characterized in that, the active ingredient of described Diaphania perspectalis sex attractant comprises the component of following parts by weight:
Along 11-hexadecenal 1 ~ 50 part
Anti-11-hexadecenal 1 ~ 20 part.
2. a kind of Diaphania perspectalis sex attractant according to claim 1, is characterized in that, the active ingredient of the attractant of described Diaphania perspectalis comprises the component of following parts by weight:
Along 11-hexadecenal 5 ~ 15 parts
Anti-11-hexadecenal 5 ~ 10 parts.
3. a kind of Diaphania perspectalis sex attractant according to any one of claim 1 or 2, is characterized in that, the active ingredient of described Diaphania perspectalis sex attractant also comprises the component of following parts by weight:
Along 11-hexadecenol 1 ~ 15 part
Along 9-hexadecenal 1 ~ 10 part.
4. a Diaphania perspectalis sex attractant lures core, it is characterized in that, described Diaphania perspectalis sex attractant lures core comprise sex attractant, solvent and lure core carrier, described sex attractant is the Diaphania perspectalis sex attractant described in any one of claims 1 to 3, and described solvent is any one in n-hexane, carrene, ether or acetone; The described core carrier that lures is that compounded rubber lures core carrier or vinyl tube.
5. a kind of Diaphania perspectalis sex attractant according to claim 4 lures core, it is characterized in that, described Diaphania perspectalis sex attractant is dissolved in described solvent, and concentration is 60 ~ 95g/L.
6. a kind of Diaphania perspectalis sex attractant according to claim 4 lures core, it is characterized in that, described Diaphania perspectalis sex attractant lures core also to comprise antioxidant and uvioresistant stabilizing agent.
7. a kind of Diaphania perspectalis sex attractant according to claim 6 lures core, it is characterized in that, described antioxidant is any one in BHA, BHT, PG; Described uvioresistant stabilizing agent be salicylate class, benzophenone class, benzotriazole, group-substituted acrylonitrile, triazines and hindered amines composite in any one.
8. a kind of Diaphania perspectalis sex attractant according to claim 7 lures core, it is characterized in that, described uvioresistant stabilizing agent is any one in phenyl salicytate, BTA, 2,4-DihydroxyBenzophenone, single benzoic acid resorcinol.
9. the Diaphania perspectalis sex attractant prepared described in any one of claim 4 ~ 8 lures the method for core, it is characterized in that, by each weight percentages of components of the Diaphania perspectalis sex attractant described in any one of claims 1 to 3, each components dissolved of getting Diaphania perspectalis sex attractant is prepared into sex attractant solution in described solvent, luring in core carrier of every 1ml drips 20 ~ 200 μ g sex attractants, add the antioxidant of 5 ~ 20 μ g and the uvioresistant stabilizing agent of 10 ~ 35 μ g, the Diaphania perspectalis sex attractant be prepared into lures core simultaneously.
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| CN112299991A (en) * | 2020-09-08 | 2021-02-02 | 宁波纽康生物技术有限公司 | Preparation method of (E, E, Z) -10,12, 14-hexadecatriene acetate, composition and lure |
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