CN108496968B - Sweet potato weevil attractant - Google Patents
Sweet potato weevil attractant Download PDFInfo
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- CN108496968B CN108496968B CN201810261943.8A CN201810261943A CN108496968B CN 108496968 B CN108496968 B CN 108496968B CN 201810261943 A CN201810261943 A CN 201810261943A CN 108496968 B CN108496968 B CN 108496968B
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- sweet potato
- attractant
- butenoate
- potato weevil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a sweet potato weevil attractant which comprises, by mass, 32-58% of (Z) -3-dodecen-1-yl (E) -2-butenoate, 10-38% of ethyl butyrate, 10-25% of cis-11-methyl dodecenoate, 5-15% of cis-3-hexeneacetate and 1% of 2, 6-di-tert-butyl-4-methylphenol, wherein the sum of the mass fractions of the substances is 100%. The (Z) -3-dodecene-1-yl (E) -2-butenoate is the main component of sex pheromone of the sweet potato weevil, and the cis-3-hexenyl acetate is not only the composition of sex information, but also can be used as a synergistic substance of the (Z) -3-dodecene-1-yl (E) -2-butenoate as the main component of the sex pheromone, so that the trapping effect of the attractant is greatly improved, the control effect on the insect population density of the sweet potato weevil larvae is better, and a new thought is developed for the comprehensive control technology of the sweet potato weevil pests.
Description
Technical Field
The invention belongs to the technical field of agricultural pest control, and particularly relates to a sweet potato weevil attractant.
Background
A sweet potato ant weevil belonging to Coleoptera order ant hierocco [ Cole ], [Cylas formicarius(Fabricius)]Is an important quarantine pest in China and abroadThe sweet potato is mainly distributed in southern sweet potato production areas and is harmful to Convolvulaceae plants such as sweet potatoes, water spinach and the like. The sweet potato weevil generates 5-8 generations in 1 year, has generation overlapping phenomenon, can be propagated in the local year in the end, and has no obvious overwintering stage. The adult crawling ability is stronger than the flying ability, and the adult crawling ability has the characteristics of pseudo death and strong hunger resistance and can last for about 150 days. The adult sweet potato weevil eats the epidermis of the potato stem and the petiole and is also harmful to tender shoots, tender leaves and leaf back governing veins, and the adult sweet potato weevil is in a trend of increasing weight year by year. China is a big sweet potato planting country, and the annual average planting area is 600 ten thousand hm2On the other hand, the annual average yield accounts for about 70% of the global yield. The sweet potato weevil is an important pest in the planting period and the storage period of the sweet potato, and the quality and the yield of the sweet potato are seriously influenced. The sweet potato weevil has the defects that the growth of the sweet potato is seriously influenced and the sweet potato is easy to cause diseases of the sweet potato except that the sweet potato weevil bites the vine, the leaf, the bud, the open sweet potato block and the storage sweet potato block by adult insects.
Insect sex pheromones are a type of trace volatile information compounds which are biosynthesized and released outside the body by specific glands after sexual maturation, thereby attracting the same kind of heterosexual individuals to carry out mating activities. Insect pheromones can regulate and control insect behaviors and play an important role in insect targeting, calling, mating, oviposition, aggregation, tracking, alarming, defense, interspecies identification and other behaviors. The insect sex pheromone can be used for insect pest situation prediction, insect pest quarantine, insect species identification and the like, and is widely used in the insect pest trapping and killing technology. At present, comprehensive control technologies such as agricultural control, application induced monitoring, trapping control and chemical agent control are used as the basis for controlling the weevil of sweet potatoes. However, no report of relevant patents of the sweet potato weevil attractant is found at present, the pheromone is comprehensively evaluated through an antenna potential reaction and a behavior selection reaction of the sweet potato weevil to each component of the pheromone, an optimized formula in field experiments and the like, the sex attractant is prepared by applying the relevant components of the sex pheromone of the sweet potato weevil, and the antioxidant 2, 6-di-tert-butyl-4-methylphenol is added, so that the attracting effect is obvious, and the attractant can be used as an important means for field monitoring and prevention and control of the sweet potato weevil.
Disclosure of Invention
The sweet potato weevil attractant provided by the invention has the characteristics of stable performance, long lasting period, high efficiency, environmental friendliness and the like, the usage amount of chemical pesticides is greatly reduced, and the attractant also has a good effect on controlling the population density of larvae.
In order to achieve the purpose, the invention adopts the following technical scheme:
the sweet potato weevil attractant comprises, by mass, 32-58% of (Z) -3-dodecen-1-yl (E) -2-butenoate, 10-38% of ethyl butyrate, 10-25% of methyl 11-dodecenoate, 5-15% of cis-3-hexeneacetic ester and 1% of 2, 6-di-tert-butyl-4-methylphenol, wherein the sum of the mass fractions of the substances is 100%.
Preferably, the components of the compound comprise, by mass, 45% of (Z) -3-dodecen-1-yl (E) -2-butenoate, 25% of ethyl butyrate, 19% of methyl 11-dodecenoate, 10% of cis-3-hexeneacetate and 1% of 2, 6-di-tert-butyl-4-methylphenol.
The preparation method of the sweet potato weevil attractant comprises the following steps: weighing the raw materials in parts by weight, and then uniformly mixing to obtain the sweet potato weevil attractant.
The (Z) -3-dodecen-1-yl (E) -2-butenoate is a main component of sex pheromone of the sweet potato weevil, and the cis-3-hexenyl acetate is a component of the sex pheromone and is a synergist of the (Z) -3-dodecen-1-yl (E) -2-butenoate serving as a main component of the sex pheromone.
The (Z) -3-dodecen-1-yl (E) -2-butenoate is (Z) -3-dodecen-1-YL (E) -2-butenoate.
The invention has the beneficial effects that:
the attractant product disclosed by the invention has the characteristics of stable performance, long lasting period, high efficiency, environmental friendliness and the like, the ethyl butyrate in the attractant has an obvious synergistic effect on the sex pheromone, the using amount of a chemical pesticide is greatly reduced, a good effect is achieved on controlling the population density of the sweet potato weevil larvae, and a new thought is developed for a comprehensive prevention and control technology of the sweet potato weevil pests.
Detailed Description
The present invention is further illustrated by the following examples, but the scope of the present invention is not limited to the following examples.
Example 1
First, the tentacle potential (EAG) reaction of sweet potato elephant nail to each component of pheromone and mixture
Uniformly dropping 20 mul of sample solution on a V-shaped filter paper strip with the length of 5cm and the width of 0.5cm, putting the filter paper strip into a Pasteur dropper, wherein the tail end of the dropper is connected with a stimulation air flow control device, and the opening of an air supply pipe is vertically vertical to the tentacle and is 1cm away from the tentacle. The continuous gas flow was adjusted to 124ml/min and the stimulation gas flow was adjusted to 20ml/min, with each stimulation time of 0.2 s. The interval between the two stimulations was 40s to ensure complete recovery of the antennal sensory functions. The components and mixtures were averaged 3 times in parallel on one antenna and at least 5 antennae were tested in duplicate. The relative EAG response values of the components and mixtures were compared with liquid paraffin. For each sample to be tested, the stimulation sequence is liquid paraffin, the sample to be tested and liquid paraffin. The two previous and subsequent references and controls were averaged.
And the EAG reaction relative value = EAG reaction value of the sample to be detected-average EAG reaction value of liquid paraffin.
The result shows that the reaction value of the sweet potato weevil to each component of the sex pheromone is (Z) -3-dodecen-1-yl (E) -2-butenoate > 11-dodecene methyl ester > ethyl butyrate > cis-3-hexeneacetate, and the relative value of the EAG reaction to the (Z) -3-dodecen-1-yl (E) -2-butenoate is obviously higher than that of other components; the EAG reaction relative value for the pheromone compound with the optimal proportion is the highest and is 1.36 +/-0.33 mv.
Secondly, the selective behavior reaction of the sweet potato weevil to the pheromone compound
The directional behavior reaction of the sweet potato weevil to the compound is measured by a Y-shaped olfactometer (the laboratory is improved according to the basic principle of the olfactometer, and the granted patent number is ZL 201020239767.7). The main arm of the Y-shaped pipe is 20cm, the lengths of the two arms are respectively 10cm, and the included angle between the two arms is 90 ℃. The two arm tubes are sequentially connected with a distilled water bottle and an active carbon bottle by a silica gel tube, and finally the active carbon bottle is opened in the atmosphere, and the insect release bottle is connected with an air extractor by gauze. The filter paper strips were cut to 5cm × 1cm, folded into a "V" shape, and placed in a trap inlet glass tube. And (3) respectively and uniformly dripping 100 mu L of the mixture obtained in the step (1.2) on a filter paper strip, putting the filter paper strip into a flavor source bottle on one side, taking the filter paper on the other side with 100 mu L of liquid paraffin as a reference, and respectively filtering air flows entering the two tubes through activated carbon and humidifying the air flows through distilled water, wherein the flow speed of the air flow is 1L/min. The adult moths with the same age are taken and placed in an insect release bottle, 20 heads are taken each time, the operation is repeated for 5 times, cotton balls are dipped into the inner wall and the outer wall of an absolute ethyl alcohol Y-shaped tube and dried after each measurement, and then two arms of the Y-shaped tube are exchanged with a taste source bottle and a taste source contrast to eliminate the possible influence of geometric errors of the two arms on the behavior of the sweet potato weevils. And (3) placing the test insects into an insect release bottle of a Y-shaped tube to start a test, counting the experimental result after 2 hours, and judging that the sweet potato weevils staying on the main arm of the Y-shaped tube are not reacted.
The attractant in the test treatment (1) comprises various components of pheromones, specifically (by mass fraction), (Z) -3-dodecen-1-yl (E) -2-butenoate 45%, ethyl butyrate 25%, 11-methyl dodecenoate 19%, cis-3-hexeneacetate 10%, and 2, 6-di-tert-butyl-4-methylphenol 1%.
The result shows that the sweet potato weevil attractant treated in the test 1 can trap 20 adult sweet potato weevils to the treatment end of the olfactometer, and the time consumption is only within 5 min.
The component of the attractant in the test treatment (2) does not contain cis-3-hexeneacetic ester, and the specific components (calculated by mass fraction) are (Z) -3-dodecen-1-yl (E) -2-butenoate 45%, ethyl butyrate 25%, 11-dodecen-oic acid methyl ester 29% and 2, 6-di-tert-butyl-4-methylphenol 1%.
The result shows that the sweet potato weevil attractant treated in the test 2 can trap 20 adult sweet potato weevils to the treatment end of the olfactometer, and the time consumption is only within 7 min.
The attractant component in the test treatment (3) does not contain 11-dodecenoic acid methyl ester, and the specific components (by mass fraction) are (Z) -3-dodecen-1-yl (E) -2-butenoate 55%, ethyl butyrate 29%, cis-3-hexeneacetic ester 15% and 2, 6-di-tert-butyl-4-methylphenol 1%.
The result shows that the sweet potato weevil attractant treated in the test 3 can trap 20 adult sweet potato weevils to the treatment end of the olfactometer, and the time consumption is only within 10 min.
The component of the attractant in the test treatment (4) does not contain ethyl butyrate, and the specific components (by mass fraction) of the attractant comprise 55% of (Z) -3-dodecen-1-yl (E) -2-butenoate, 29% of methyl 11-dodecenoate, 15% of cis-3-hexeneacetate and 1% of 2, 6-di-tert-butyl-4-methylphenol.
The result shows that the sweet potato weevil attractant treated in the test 4 can completely trap 20 adult sweet potato weevils to the treatment end of the olfactometer, and the time consumption is only within 10 min.
In the test treatment (5), the attractant component does not contain (Z) -3-dodecen-1-yl (E) -2-butenoate, and the specific components (by mass fraction) comprise 30% of ethyl butyrate, 39% of methyl 11-dodecenoate, 30% of cis-3-hexeneacetate and 1% of 2, 6-di-tert-butyl-4-methylphenol.
The result shows that the attracting amount of the sweet potato weevil attractant for adult sweet potato weevils treated in the test 5 is 6.48 +/-0.78 heads within 10min, and the rest sweet potato weevils stay on the main arm of the Y-shaped olfactometer.
The experiment shows that (Z) -3-dodecen-1-yl (E) -2-butenoate is a main component of sweet potato weevil sex pheromone, ethyl butyrate and 11-dodecenoic acid methyl ester are minor components, and cis-3-hexeneacetate is a minor component and can be used as a synergist of the main component.
Example 2
The components are weighed according to the mixture ratio of each component in the formula 1, the formula 2, the formula 3, the formula 4, the formula 5 and the CK sample described in the table 1, and then are respectively and uniformly mixed to obtain six experimental formulas of 1, 2, 3, 4, 5 and CK.
Table 1: components of attractant formula and proportion thereof
The core inducing carrier used in the experiment is a cuff type composite rubber head, firstly, six formulas 1, 2, 3, 4, 5 and CK are mixed according to the proportion and the dosage in the table 1, normal hexane is used as a solvent, a formula sample is quantitatively added into the composite rubber head, after the normal hexane is volatilized, the mixture is sealed and uniformly mixed to prepare the core inducing carrier, and the core inducing carrier is stored at a low temperature in a refrigerator for later use.
The trapper adopts a yellow viscose triangular trapper. All field trials were conducted during the peak period of adult weevils in sweetpotato. When the vines grow to about 5-10 cm, lure cores are placed 3-5cm above the triangular traps, the trap lures pots are placed randomly in the field, each treatment is set up for 3 replicates in the field, each lure is arranged in a zigzag fashion, and each trap is about 10-15m apart. Every 3 days for each different treatment, the viscose plates are changed, the number of the lured insects is counted, and meanwhile, the influence of the positions on the insect luring effect is reduced by changing the positions of the traps. The lure was changed 1 time every 1 month while the position of the trap was changed. 1 control area is arranged in the sweet potato field 50 m away from the test area. The results are shown in Table 2 by experimental statistics:
table 2: comparison of field insect-attracting effects of the test samples
As shown in table 2, the attractant formulas 1, 2, 3, 4, 5 all had significantly higher trapping numbers for sweet potato weevils than the yellow sticky plates used alone; the formula 4 (the preferable formula in the invention) has the largest attraction amount, the formula 3, 4 and 5 have the insect attraction amount obviously higher than that of the formula 1, and the formula 2 has the insect attraction amount higher than that of the formula 1, so that cis-3-hexeneacetic ester plays a certain attraction synergistic effect on the weevil. Therefore, the optimized formula provided by the invention has an obvious attraction effect on the imagoes of the sweet potato weevil.
The embodiments described in this specification are merely illustrative of implementations of the inventive concept and the scope of the present invention should not be considered limited to the specific forms set forth in the embodiments but on the basis of equivalents thereof which may occur to those skilled in the art based on the inventive concept.
Claims (2)
1. An attractant for sweet potato weevil is characterized in that: the composition comprises, by mass, 32-58% of (Z) -3-dodecen-1-yl (E) -2-butenoate, 10-38% of ethyl butyrate, 10-25% of methyl 11-dodecenoate, 5-15% of cis-3-hexeneacetate and 1% of 2, 6-di-tert-butyl-4-methylphenol, wherein the sum of the mass fractions of the above substances is 100%.
2. The attractant for sweet potato weevil according to claim 1, which is characterized in that: the components of the compound are (Z) -3-dodecen-1-yl (E) -2-butenoate 45%, ethyl butyrate 25%, 11-dodecenic acid methyl ester 19%, cis-3-hexeneacetic ester 10% and 2, 6-di-tert-butyl-4-methylphenol 1% in percentage by mass.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810261943.8A CN108496968B (en) | 2018-03-28 | 2018-03-28 | Sweet potato weevil attractant |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810261943.8A CN108496968B (en) | 2018-03-28 | 2018-03-28 | Sweet potato weevil attractant |
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| CN108496968A CN108496968A (en) | 2018-09-07 |
| CN108496968B true CN108496968B (en) | 2020-05-26 |
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| CN201810261943.8A Active CN108496968B (en) | 2018-03-28 | 2018-03-28 | Sweet potato weevil attractant |
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| CN113040145B (en) * | 2021-03-12 | 2022-04-08 | 虫捕头(苏州)生物科技有限公司 | Preparation method of elm spot moth sex attractant |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4732756A (en) * | 1986-06-27 | 1988-03-22 | The United States Of America As Represented By The Secretary Of Agriculture | (Z)-3-dodecen-1-ol (E)-2-butenoate and its use in monitoring and controlling the sweetpotato weevil |
| CN105994213A (en) * | 2016-05-20 | 2016-10-12 | 陈超 | Insect trapping device and insect trapping method |
| CN107535533A (en) * | 2017-09-27 | 2018-01-05 | 福州市农业科学研究所 | A kind of method for walking quickly and keeping away cylas formicarius |
-
2018
- 2018-03-28 CN CN201810261943.8A patent/CN108496968B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4732756A (en) * | 1986-06-27 | 1988-03-22 | The United States Of America As Represented By The Secretary Of Agriculture | (Z)-3-dodecen-1-ol (E)-2-butenoate and its use in monitoring and controlling the sweetpotato weevil |
| CN105994213A (en) * | 2016-05-20 | 2016-10-12 | 陈超 | Insect trapping device and insect trapping method |
| CN107535533A (en) * | 2017-09-27 | 2018-01-05 | 福州市农业科学研究所 | A kind of method for walking quickly and keeping away cylas formicarius |
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