CN113678827B - Attractant composition for black cutworm larvae and application thereof - Google Patents
Attractant composition for black cutworm larvae and application thereof Download PDFInfo
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- CN113678827B CN113678827B CN202111039421.1A CN202111039421A CN113678827B CN 113678827 B CN113678827 B CN 113678827B CN 202111039421 A CN202111039421 A CN 202111039421A CN 113678827 B CN113678827 B CN 113678827B
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- black cutworm
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses an attractant composition for black cutworm larvae and application thereof. The paint comprises the following components in parts by weight: 0.1 to 0.8 portion of 2-ethyl hexanol, (2E, 6E, 11Z) -3,7,13-trimethyl-10- (2-propyl) -2,6,11-cyclotetradecatriene-1,13-diol, 0.08 to 0.3 portion of undecane, 1 to 3 portions of styrene, 0.8 to 2.5 portions of dibutyl phthalate and 0.1 to 0.8 portion of hexadecane. The synergist provided by the invention has low component price, can reduce the cost for manufacturing the lure and is suitable for popularization and promotion.
Description
The technical field is as follows:
the invention belongs to the technical field of pest monitoring and trapping, and particularly relates to an attractant composition for black cutworm larvae and application thereof.
Background art:
agrotis ipsilon belongs to the Lepidoptera Noctuidae family, is an important underground pest, has wide host plants, and can almost eat and damage seedlings of all plants except aquatic plants such as rice and the like. The larvae are main pests in the seedling stage of the tobacco, and the entire tobacco is dead and harmful by gnawing the stems of the seedlings, so that the seeds are damaged in severe cases. The prevention and treatment method for underground insect pests still mainly uses chemical pesticides at present, but chemical agents cause environmental pollution due to high toxicity and high dosage; the green prevention and control of the ecological method can reduce the use of chemical pesticides and protect the health of human beings.
For example, the compound which can definitely attract the black cutworm larvae is beneficial to preventing the root cutting behavior of the black cutworm and maintaining the crop production. The current common trapping technology for noctuidae ecology mainly comprises sex attraction and food attraction technologies for adults and sugar-vinegar wine liquid bait technologies for larvae, but has the problems of high cost of manpower and material resources, poor stability and difficulty in large-area popularization of the larvae.
The existence of ideal trapping effect has certain influence on the insect killing, and the food attractant meets the requirement of the insect on favorite food to achieve the effect of gathering the insect at the position where the chemical agent directly acts. The food attractant can gather the agrotis ypsilon so as to play a role in monitoring and increasing the prevention and treatment effect. Compared with the attraction of sugar, wine and vinegar, the food attractant is more selective and lasting, and the operation is simple and convenient and is easy to popularize. Pheromones are only effective on males and cannot detect, prevent and control larvae which are really harmful. In addition, the food attractant can be used together with other medicaments or means, so that the pest can be killed efficiently and intensively. In actual production, chemical control is usually carried out after pests appear, and the feeding attractant can trap and kill the pests in advance, so that the time and the quantity of the pests damaging agricultural production are reduced.
Therefore, it is desirable to provide a lure containing the agrotis ypsilon larva attractant composition, so as to alleviate the problems of high cost, difficult operation and difficult popularization, and provide diversified options for the monitoring and prevention and control technology of the agrotis ypsilon larva. .
Disclosure of Invention
The invention aims to overcome the defects that the cutworm larvae cannot be monitored and intensively prevented and controlled in the existing noctuid trapping technology, the labor cost is high, and the popularization is difficult, and provides a food attractant composition for the cutworm larvae and application thereof, so that the labor cost input is reduced, the trapping activity is improved, and the centralized killing is realized, thereby reducing the assistance and the field production cost.
The invention provides an attractant composition for black cutworm larvae, which comprises the following components in parts by weight: 0.1 to 0.8 portion of 2-ethyl hexanol, (2E, 6E, 11Z) -3,7,13-trimethyl-10- (2-propyl) -2,6,11-cyclotetradecatriene-1,13-diol 2 to 8 portions, 0.08 to 0.3 portion of undecane, 1 to 3 portions of styrene, 0.8 to 2.5 portions of dibutyl phthalate and 0.1 to 0.8 portion of hexadecane.
Preferably, the attractant composition comprises the following components in parts by weight: 0.4 to 0.8 portion of 2-ethyl hexanol, (2E, 6E, 11Z) -3,7,13-trimethyl-10- (2-propyl) -2,6,11-cyclotetradecatriene-1,13-diol 5 to 8 portions, 0.1 to 0.3 portion of undecane, 2.2 to 3 portions of styrene, 1.6 to 2.5 portions of dibutyl phthalate and 0.5 to 0.8 portion of hexadecane.
Further preferably, the attractant composition comprises the following components in parts by weight: 0.4 part of 2-ethylhexanol, (2E, 6E, 11Z) -3,7,13-trimethyl-10- (2-propyl) -2,6,11-cyclotetradecatriene-1,13-diol 5.1 parts, 0.1 part of undecane, 2.2 parts of styrene, 1.6 parts of dibutyl phthalate and 0.5 part of hexadecane.
A second object of the present invention is to provide the use of the attractant composition for black cutworm larvae as described above for attracting, controlling and/or monitoring black cutworm.
Preferably, the application comprises applying the attractant composition for black cutworm larvae onto a blank lure to attract black cutworm larvae; or applying the attractant composition of the black cutworm larvae on a blank attracting core, and then matching the attracting core with chemical pesticide/biological pesticide for use.
The third purpose of the invention is to provide a method for preventing and/or monitoring black cutworm larvae, which is to apply the attractant composition for the black cutworm larvae to the habitat of the black cutworm and attract the black cutworm.
Preferably, the use amount of the attractant composition for the black cutworm larvae is 0.6 to 10 mg/square meter.
Compared with the prior art, the invention has the advantages that:
(1) The use mode is simple and flexible. The attractant composition for the black cutworm larvae, provided by the invention, has the advantages that various components are jointly used according to the proportion for monitoring, or are matched with pesticides for centralized killing for preventing and treating, and the autonomy is stronger.
(2) Compared with the traditional luring materials for larvae, such as sweet and sour wine, and the like, the luring core containing the agrotis ypsilon attractant has stronger persistence in the field, and obviously reduces the manpower for monitoring/preventing.
(3) The synergist provided by the invention has low component price, can reduce the cost for manufacturing the lure and is suitable for popularization and promotion.
Detailed Description
The present invention is described in further detail below with reference to specific embodiments, and the examples are given only for illustrating the present invention and not for limiting the scope of the present invention.
Example 1
The test of the indoor attraction effect of the tiger were conducted on the attracting core of the agrotis ypsilon attractant at the animal research institute of academy of sciences of Guangdong province, 7 months and 5 days to 15 days in 2021.
The components of the attractant are as follows: the purity of 2-ethyl hexanol, (2E, 6E, 11Z) -3,7,13-trimethyl-10- (2-propyl) -2,6,11-cyclotetradecatriene-1,13-diol, undecane, styrene, dibutyl phthalate and hexadecane is above 99%.
Respectively preparing 2-ethylhexanol, (2E, 6E, 11Z) -3,7,13-trimethyl-10- (2-propyl) -2,6,11-cyclotetradecatriene-1,13-diol, undecane, styrene, dibutyl phthalate and hexadecane solutions with the concentrations of 5 mg/ml and 10 mg/ml by using normal hexane as a solvent, respectively dropwise adding 200 microliters of the solution onto a partition of a rubber plug cleaned by the normal hexane, and obtaining the attractant core after the normal hexane is volatilized. The prepared attractant core is packaged in aluminum foil for sealing, and is stored at-20 deg.C.
The components are mixed according to the composition in the table 1 and dissolved in n-hexane to prepare n-hexane solution of the multi-component synergist, the concentrations of the solutions are respectively 5 mg/ml and 10 mg/ml, 200 microliters of the solution is respectively dripped on a spacer of a rubber plug cleaned by n-hexane, and after the n-hexane volatilizes, the attractant core is prepared. The prepared multi-component attractant core is put into an aluminum foil for sealing and stored at the temperature of minus 20 ℃.
Selecting nearly all agrotis parvus larvae to starve for 2 hours in a box, placing a single component or combined lure core at a random position along the inner wall in a 9 cm culture dish, and placing a contrast lure core dropwise added with 200 microliters of n-hexane at the opposite side. Gently clamping one end of the low-age agrotis ypsilon by using forceps, placing the lower-age agrotis ypsilon in the center of the culture dish, covering the culture dish, timing for 3 minutes, and recording the selection condition of the larva on the lure core. And (3) replacing a pair of lure cores for each 15 head worm, and corresponding to a pair of screened lure cores for each culture dish. If the agrotis ypsilon is attracted by the attractant, the agrotis ypsilon will move forward to the attracting core of the attractant. Each component to be screened was repeated 30 times. The test results are shown in table 2 (suction rate (%) = (number of suction heads/total number of test heads) X100).
In the test data of Table 2, groups A-1 to F-2 are the effects of lure made using a single compound; the M-1 and M-2 groups are the effect of the attractant composition for the self-prepared black cutworm larvae to lure the core. As can be seen from the table, after the attractant composition containing the black cutworm larvae of the present example was used to lure the core, the attraction rate of a single compound to the black cutworm larvae reached 54.5% to 80.0%, while the attraction rate of the composition to lure the core was even stronger, reaching 84.6% to 87.5%.
TABLE 1 composition of multicomponent attractants
TABLE 2 test data
Example 2
The agrotis ypsilon attractant comprises the following components in parts by weight: 0.8 part of 2-ethylhexanol, (2E, 6E, 11Z) -3,7,13-trimethyl-10- (2-propyl) -2,6,11-cyclotetradecatriene-1,13-diol 8 parts, 0.3 part of undecane, 3 parts of styrene, 2.5 parts of dibutyl phthalate and 0.8 part of hexadecane are mixed according to parts by weight and dissolved in n-hexane to prepare a n-hexane solution of the multi-component attractant, the concentration of the solution is 20 mg/ml, 200 microliters of the n-hexane solution is dripped on a partition of a rubber plug cleaned by n-hexane, and the synergist attractant core is prepared after the n-hexane is volatilized. The prepared multi-component attractant core is put into an aluminum foil for sealing and stored at the temperature of minus 20 ℃.
Selecting wet or just raining and proper temperature (about 25 ℃) in Huaning county of Yuxi city of Yunnan province from 2021, 5 months and 3 days to 14 days to carry out a test in a tobacco field, taking 1 attractant core of multi-component attractant from an attraction area, placing the attractant core on an annular area of 8 centimeters per tobacco seedling at random, not placing the attractant core in a contrast area, and then using the attractant coreChemical pesticides (Dishimei, a product of Bayer AG, germany), biopesticides (Steinernema carpocapsae All powder, available from animal institute of academy of sciences, guangdong province) and clear water, each cell was set up for 3 replicates, each replicate investigating 15 tobacco plants. Adding 2 artificially-fed 4-year-old black cutworm larvae into the diagonal angle of an area with each tobacco seedling as the center of 8 cm, and covering the pond with fine dry soil. The dosage of the biological pesticide is 2 multiplied by 10 per tobacco 5 A head; the dosage of the chemical pesticide is 4 ml/mu, 15 kg of water is added, and 14 g of the chemical pesticide is sprayed on each cigarette. And (4 d), 7d and 10d after transplanting treatment, respectively investigating the damage condition of the tobacco seedlings, wherein the damage rate (%) = (the number of the tobacco seedlings damaged by the black cutworms/the number of the total tobacco seedlings treated) × 100. The results of the field tests are shown in Table 3.
As can be seen in the test data of table 3, the harm rate of the biopesticides and the chemical pesticides for controlling the tobacco seedlings is remarkably improved after the attractant core containing the agrotis ypsilon of the embodiment is used; in addition, the control of the harm rate of tobacco by each treatment can be obviously prolonged by using the cell containing the attractant core of the agrotis ypsilon of the embodiment.
Table 3 field test data
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.
Claims (5)
1. The attractant composition for the black cutworm larvae is characterized by comprising the following components in parts by weight: 0.4 part of 2-ethylhexanol, (2E, 6E, 11Z) -3,7,13-trimethyl-10- (2-propyl) -2,6,11-cyclotetradecatriene-1,13-diol 5.1 parts, 0.1 part of undecane, 2.2 parts of styrene, 1.6 parts of dibutyl phthalate and 0.5 part of hexadecane.
2. Use of the attractant composition for black cutworm larvae according to claim 1 for attracting, controlling and/or monitoring black cutworm.
3. The use according to claim 2, wherein the use is the application of the attractant composition for black cutworm larvae onto a blank lure to attract black cutworm larvae; or the attractant composition of the black cutworm larvae is applied to the blank attracting core, and then the attracting core is matched with the chemical pesticide/biological pesticide for use.
4. A method for controlling and/or monitoring black cutworm larvae, wherein the attractant composition for black cutworm larvae according to claim 1 is applied to the habitat of black cutworm to lure black cutworm.
5. The method as claimed in claim 4, wherein the attractant composition for the larvae of black cutworm is used in an amount of 0.6-10 mg/m.
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| CN202111039421.1A CN113678827B (en) | 2021-09-06 | 2021-09-06 | Attractant composition for black cutworm larvae and application thereof |
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| CN202111039421.1A CN113678827B (en) | 2021-09-06 | 2021-09-06 | Attractant composition for black cutworm larvae and application thereof |
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| CN113678827B true CN113678827B (en) | 2023-03-24 |
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| CN107439545B (en) * | 2017-08-13 | 2020-02-18 | 中国农业科学院植物保护研究所 | Attractant composition for preventing and treating black cutworm and application thereof |
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