CN110720456B - Application of 3, 5-dimethoxytoluene - Google Patents
Application of 3, 5-dimethoxytoluene Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses application of 3, 5-dimethoxytoluene, and the application of the 3, 5-dimethoxytoluene is the application of the 3, 5-dimethoxytoluene in preparation of an insect attractant. The 3, 5-dimethoxytoluene is one of volatile secondary metabolites synthesized by Chinese rose plants, the active ingredient is separated from Chinese rose headspace volatiles, and the 3, 5-dimethoxytoluene has good attraction effect on pests, particularly spodoptera exigua moths, through olfactory behavior, oviposition behavior biological tests and field oviposition tests. And 3, 5-dimethoxytoluene is a natural secondary metabolite and has a complete degradation way in the nature, so that the 3, 5-dimethoxytoluene is used as a main component to prepare the pest attractant, thereby realizing the pollution-free control of pests.
Description
Technical Field
The invention belongs to the technical field of plant protection, and particularly relates to application of 3, 5-dimethoxytoluene.
Background
At present, chemical pesticides are one of the main means for controlling plant pests, however, while chemical pesticides, especially chemical pesticides, can eliminate pests, the problems of environmental pollution, pest resistance, pesticide residue exceeding standards of agricultural products and the like can be caused. With the increasing requirements of modern agriculture and ecological environment on the performance of chemical pesticides, the research and development risks of new chemical pesticide varieties are also increasing. The botanical pesticide has the advantages of various biological activities (various modes of killing insects, killing bacteria and weeding), high selectivity, no residue, safety to the environment, safety to higher animals and natural enemies, difficulty in generating drug resistance to pests and the like, and is a hotspot of research by people at present.
Beet armywormSpodoptera exiguaThe (Hubner) belongs to Lepidoptera and Noctuidae, is a worldwide intractable pest, and particularly has serious damage to beet armyworm in the greenhouse production process of China rose (namely rose). Earlier researches find that the mixture of volatile components of Chinese rose is extracted by adopting a headspace dynamic adsorption method and is used for testing the influence of the mixture on the olfactory behaviors of the beet armyworm adults, and the results show that the mixture has an obvious regulation and control effect on the olfactory behaviors and has attraction activity on unmated females, mated females and male moths of the beet armyworm. Therefore, these volatile compounds are likely to be one of the important information compounds for beet armyworm to recognize host plants. The compounds are used as secondary metabolites of plants, have a complete degradation path in the nature, and play an important role in the pollution-free control of pests if researches prove that the compounds have stronger activity on certain pests.
In addition, the mixture was found to contain 3, 2-dimethoxytoluene by GC-MS identification analysis, but it was not clear whether the compound was active against Spodoptera exigua.
The existing method for preventing and controlling beet noctuids mainly uses sex pheromone to trap male moths to kill the male moths, except chemical prevention and control. But has two problems, one is that the asparagus caterpillar has parthenogenesis phenomenon; secondly, even if only a few male moths are not killed, the mating requirement can be met, and the population density cannot be effectively reduced. Therefore, the control of the female moth is very important. Therefore, a pest attractant which uses an information compound synthesized by a host plant as a main component is developed and developed for regulating and controlling the host searching behavior and the spawning behavior of female moths, so that the moths can be more effectively controlled.
Disclosure of Invention
The invention aims to provide application of 3, 5-dimethoxytoluene.
The purpose of the invention is realized by the application of the 3, 5-dimethoxytoluene in preparing the insect attractant.
The 3, 5-dimethoxytoluene is one of volatile secondary metabolites synthesized by Chinese rose plants, the active ingredient is separated from Chinese rose headspace volatiles, and the 3, 5-dimethoxytoluene has good attraction effect on pests, particularly spodoptera exigua moths, through olfactory behavior, oviposition behavior biological tests and field oviposition tests. And 3, 5-dimethoxytoluene is a natural secondary metabolite and has a complete degradation way in the nature, so that the 3, 5-dimethoxytoluene is used as a main component to prepare the pest attractant, thereby realizing the pollution-free control of pests.
Drawings
FIG. 1 shows the GC-EAD reaction of spodoptera exigua linnaeus female moths on volatile components of healthy Chinese roses;
FIG. 2 shows the EAG reaction of spodoptera exigua linnaeus with 3, 5-dimethoxytoluene;
FIG. 3 shows the olfactory reaction of spodoptera exigua ovi on 3, 5-dimethoxytoluene;
in the figure, the calculation formula of the attraction rate in the biological test of the olfactory behaviors is as follows
AP(%)＝(N T－N CK)/( N T + N CK)×100%，
Wherein,APto attract rate (attracting percentage), N Tthe number of female moths on the side of 3, 5-dimethoxytoluene,N CKthe number of female moths on the ethanol control side,N Nthe number of unreacted insects is shown; by means of x2The tests were subjected to a statistical analysis which,P <0.05 indicated significant difference;
FIG. 4 is a graph showing the effect of 3, 5-dimethoxytoluene on the oviposition behavior of spodoptera exigua;
in the figure, different lower case letters indicate that 3, 5-dimethoxytoluene is significantly different from the Control (CK) (CK)P ≤ 0.05)。
Detailed Description
The present invention is further illustrated by the following examples and the accompanying drawings, but the present invention is not limited thereto in any way, and any modifications or alterations based on the teaching of the present invention are within the scope of the present invention.
The application of the 3, 5-dimethoxytoluene is the application of the 3, 5-dimethoxytoluene in preparing the pest attractant.
The effective concentration of the 3, 5-dimethoxytoluene in the pest attractant is 0.4-50.0 g/L.
The effective concentration of the 3, 5-dimethoxytoluene in the pest attractant is 10.0 g/L.
The pests are beet armyworms.
The pests are beet armyworm female moths.
The preparation method of the pest attractant comprises the steps of preparation of a mother solution A, preparation of an auxiliary solution B and preparation, and specifically comprises the following steps:
A. preparing a mother solution A: accurately weighing 3, 5-dimethoxytoluene, adding the 3, 5-dimethoxytoluene into a solvent for dissolving to prepare mother liquor A;
B. preparing an auxiliary liquid B: preparing a tween-80 solution by using deionized water to obtain an auxiliary solution B;
C. preparation: and mixing the mother solution A and the auxiliary solution B, carrying out ultrasonic treatment, and then fixing the volume to obtain the target pest attractant.
The solvent in the step A is one or more of ethanol, normal hexane, methanol and dichloromethane.
And B, the mass percentage concentration of the Tween-80 solution in the step B is 0.1%.
And the ultrasonic treatment in the step C is ultrasonic treatment for 4-6 min at the ultrasonic frequency of 40-60 KHz.
The use method of the pest attractant is that the pest attractant is diluted by 0-125 times with ethanol or water and then directly and uniformly sprayed on buds, branches and leaves of plants.
The pest attractant has the best effect of attracting beet armyworms, particularly female moths.
The working principle of the invention is as follows:
china rose (Rosa chinensisJacq, commonly known as rose)Is one of the four fresh cut flowers in the world, is popular with people and is widely cultivated all over the world. However, the occurrence of diseases and insect pests in China rose is also very serious, and mainly comprises the Chinese rose powdery mildew [ 2 ]Sphaerotheca pannosa（Wallr. ：Ex Fr.）Lév.]Beet armyworm [ sic ], [ solution of Japanese asparagus ] andSpodoptera exigua（Hübner）]and the like. Beet armyworm belongs to Lepidoptera and Noctuidae, is a worldwide intractable pest, and particularly has serious harm to beet armyworm in the greenhouse production process of China rose (namely rose). It is well known that plant volatile components play a crucial role in insect host selection. Earlier researches find that the mixture of volatile components of Chinese rose is extracted by adopting a headspace dynamic adsorption method and is used for testing the influence of the mixture on the olfactory behaviors of the beet armyworm adults, and the results show that the mixture has an obvious regulation and control effect on the olfactory behaviors and has attraction activity on unmated females, mated females and male moths of the beet armyworm. Therefore, these volatile compounds are likely to be one of the important information compounds for beet armyworm to recognize host plants. The compounds are used as secondary metabolites of plants, have a complete degradation path in the nature, and play an important role in the pollution-free control of pests if researches prove that the compounds have stronger activity on certain pests. Therefore, the volatile components of the Chinese rose are further separated and identified by GC-MS, and 3, 5-dimethoxytoluene in the volatile components is found to be one of the main active components generating attraction effect.
The invention has the characteristics that:
3, 5-dimethoxytoluene is used as an information compound for identifying a host plant female moth and is used for replacing chemical pesticides to control the beet armyworm, and the beet armyworm can not generate adverse effect on Chinese rose plants and can not cause the beet armyworm to generate drug resistance. Meanwhile, the 3, 5-dimethoxytoluene comes from host plants, has a natural and complete degradation way in nature, does not generate adverse effect on the environment, is an environment-friendly active component, and has wide application prospect.
Example 1
Discovery of pest-attracting substance
(1) Extraction and identification of volatile components
Collecting healthy volatile components of Chinese rose leaves without diseases and insect pests by adopting a headspace dynamic adsorption method, storing the collected volatile components in a refrigerator at the temperature of-20 ℃, and carrying out GC-MS analysis. The adsorbent used in the collection process is Tenax TA, and the collection time is 24 hours, so as to eliminate the influence of the change of the daily rate on the types and the contents of the volatile components.
(2) Method for breeding beet noctuids
Putting the collected beet armyworm mature larva into a 250 mL beaker padded with damp sand, sealing the beaker by using gauze, and transferring the adult insects into another 250 mL beaker after pupation and eclosion. The nutrition is supplemented with 10% honey water, so that the Chinese rose leaves can lay eggs and hatch on healthy Chinese rose leaves, and the Chinese rose leaves are raised to mature larvae (raising conditions: 27 ℃, 70% RH, 12 h photoperiod). Pupating by the same method, taking pupa out under a scope, and breeding female pupa and male pupa separately, wherein the pupa is used for biological test after eclosion.
(3) GC-EAD test of beet armyworm adults
The temperature rise program of the gas chromatograph is consistent with that of the GC-MS; and (4) sampling without shunting, wherein the sampling amount is 2.0 mu L. The recorded signals are connected via an amplifier (Syntech CS-55) to a converter IDAC (Syntech IDAC-2) which is in turn connected to a hardware acquisition card of a computer, and data are acquired and analyzed by computer software (GC-EAD, version 4.6). The tentacles of the beet armyworm adults are cut off from the base part, 2 flagellates at the end part are removed, and then the tentacles are connected with an antennal potentiostat probe by conductive adhesive. The chromatographic column is HP-5 capillary column (30 m × 0.25 mm × 0.25 μm), the outlet is connected with shunt, the split ratio is 1: 1, one part is introduced into FID hydrogen flame ionization detector, and the other part is blown to antenna (EAD) through heating jacket (Syntech MP-3), and GC-EAD software is used for synchronously recording gas chromatogram and antenna potential diagram. The test insect status is that the beet armyworm has mated female moth. The used antenna is not reused.
(4) Results
The GC-MS identification results are shown in Table 1. The results showed that the natural compounds collected from healthy Chinese rose plants contained 3, 5-dimethoxytoluene (Table 1, No. 7). Meanwhile, the GC-EAD results (fig. 1) indicate that peak No. 1 in fig. 1 produces a clear antennal Electrophysiological (EAG) signal. According to FID information such as retention time of the No. 1 peak in GC-EAD, combining all other peaks, and carrying out comparison analysis with GC-MS, the No. 1 peak is 3, 5-dimethoxytoluene. The compound can generate an EAG signal, and the compound is one of important information compounds for identifying hosts and searching spawning sites of the spodoptera exigua female moths, so that the activity of the compound can be further researched.
TABLE 1 GC-MS analysis of volatile constituents of healthy China roses
Table 1 GC-MS analysis for volatiles collected from healthy rose plants
Example 2
EAG reaction of beet noctuid imago on 3, 5-dimethoxytoluene
And (3) cutting off tentacles of the spodoptera exigua female moths, removing 2 flagellates at the end parts, connecting the cut tentacles with a probe of an tentacle potentiostat by using conductive adhesive, and carrying out an tentacle Electrophysiological (EAG) test. 20.0. mu.L of the sample solution to be tested (3, 5-dimethoxytoluene-n-hexane solution) was applied uniformly to a filter paper strip (l.times.w = 6.0. times.0.5 cm), placed in a Pasteur tube and connected to a stimulating gas stream. The flow rate of the stimulation gas flow was 1150 mL/min and the flow rate of the continuous gas flow was 850 mL/min. After the baseline is stabilized, the foot pedal is used for artificial stimulation, the single stimulation lasts for 0.2s, and the interval is more than 60s each time. The sequence of the sample to be detected in the same antenna is n-hexane (1 time), trans-2-hexenal (3 times), the sample to be detected (3 times) and n-hexane (1 time). Wherein n-hexane is used as a control; the trans-2-hexenal is a standard compound, and the concentration of the sample to be tested and the concentration of the standard compound are both 50.0 g/L. The order of detection of the standard compound and the sample was interchanged after each 1 antenna tested. Each sample was replicated with 4 antennae. The EAG signal was amplified by an amplifier (Syntech CS-55), displayed on an oscilloscope, and normalized by software provided by Syntech corporation for the EAG response value of each sample, and the EAG relative response value = (response value of sample-response value of control)/(response value of standard compound-response value of control) × 100%. Four solutions of 3, 5-dimethoxytoluene with different concentrations (0.4, 2.0, 10.0, 50.0 g/L) were tested in the same manner and the results are shown in the attached FIG. 2.
As can be seen from FIG. 2, 3, 5-dimethoxytoluene caused a significant EAG reaction. When the spodoptera exigua linza female moth is used for testing, the relative reaction value of EAG is over 100 percent, which shows that the spodoptera exigua linza 3, 5-dimethoxytoluene has stronger reaction capability than that of a standard compound trans-2-hexenal, even if the concentration is the lowest (0.4 g/L), the relative reaction value of EAG reaches 120.7 percent. However, as the concentration of 3, 5-dimethoxytoluene increased, the relative reaction value of EAG did not increase proportionally, and the difference was not great when comparing the concentrations of 10.0 and 50.0 g/L.
In the embodiment, the standard 3, 5-dimethoxytoluene is purchased, and the compound is tested to confirm that the compound has activity on spodoptera exigua and plays an important role in the selection process of spodoptera exigua egg-laying places of spodoptera exigua. It is still unclear whether the compound has an attracting effect or a repelling effect on the beet armyworm adults.
Example 3
Olfactory behavior of spodoptera exigua on 3, 5-dimethoxytoluene
The main instrument used is a Y-type olfactometer. Wherein the main branch of the Y-shaped pipe is 22 cm, the branches at two sides are 17 cm, the inner diameter is 5 cm, and the included angle between the two arms is 75 degrees. Cleaning the Y-shaped tube, sterilizing with absolute ethyl alcohol, and drying. A Y-type olfactometer is connected with a silicone tube according to the sequence of an air pump, activated carbon, distilled water, an LZB-II glass rotameter, a three-way pipe and a Y-type pipe, and is horizontally placed, black cloth shades the light, and the air flow is adjusted to be 300 mL/min. The airflow is converged on the main branch of the Y-shaped pipe and flows out from the opening of the base. Clean air was introduced for 10 minutes before the experiment was started.
In order to make the gas slowly overflow, a small glass bottle with a small hole and containing 0.4 g/L of 3, 5-dimethoxytoluene solution (prepared by n-hexane) is placed into one side branch of a Y-shaped olfactometer, the n-hexane is used as a control for the other side branch, mated female moths are placed into the small glass bottle from the base port of the Y-shaped tube, 10 moths are placed each time, and the small glass bottle is sealed by black yarns with small holes of 2 mm to prevent adult moths from escaping. The moth can climb or fly to the smell source in a reversible wind manner, identify the smell source and make a response. The number of unreacted insects in the branches at both sides of the Y-shaped tube and in the basal part at 30 min was recorded, and positive reaction was recorded when the insects advanced 3 cm or more from the intersection of the branches at both sides. 2.0, 5.0 and 10.0g/L of the solution are tested by the same method. The test was repeated 5 times, the mean value was taken, and statistical analysis was performed on SPSS 17.0.
After each live test, the direction of the Y-tube and the odor source are exchanged. After the two tests, the Y-shaped tube and the flavor source bottle are taken down, cleaned, disinfected, dried and then subjected to the next bioassay. The used female moth is not reused. The bioassay is carried out at a time interval of 7: 00-12: 00 at night under the conditions of no wind, room temperature of 25 ℃, shading and air humidity of 75%. From the entrance of the base part to the odor source, 3, 5-dimethoxytoluene forms a concentration gradient from small to large, the spodoptera exigua mating female moth can identify the type of the information compound and the concentration gradient thereof and make corresponding reaction, namely the principle is consistent with the principle that the spodoptera exigua identifies the host plant in the nature.
The test results are shown in FIG. 3. As can be seen from figure 3, in the olfactory behavior test, 3, 5-dimethoxytoluene shows an obvious attraction effect on spodoptera exigua female moths, and the attraction effect is better when the mass concentration is higher. When the mass concentration is 50.0g/L, the attraction rate is maximum and reaches 55.8 percent. Similar to the EAG test results, the differences were not significant when tested at concentrations of 10.0 and 50.0g/L (P= 0.864), the attraction rate was substantially uniform. Compared with the concentrations of 10.0 and 2.0 g/L, the difference is obvious and reaches a significant level (a)P = 0.044）。
Example 4
Attraction Activity test of 3, 5-Dimethoxytoluene
Sterile soil of about 5 cm thickness was placed in a 2-piece 500 mL beaker and moistened with a suitable amount of sterile water. Two bundles of 5 healthy and disease and insect pest free fresh Chinese rose branches with the length of about 20 cm and the number of leaves basically the same are respectively inserted into the soil in 2 beakers, and the two beakers are respectively marked as No. 1 and No. 2. Using ethanol as solvent to prepare 0.4 g/L solution of 3, 5-dimethoxytoluene, uniformly spraying 5 mL of the solution on branches and leaves in No. 1 beaker, and spraying 5 mL of ethanol on branches and leaves in No. 2 beaker by the same method as a control. The two beakers were placed in an egg laying cage made of fine wire mesh at a distance of about 50 cm.
Artificially feeding beet armyworms until pupation, taking the pupae out, distinguishing male and female pupae under a scope, and separately feeding the male and female pupaes until eclosion. 10 pairs of adult beet armyworms which have eclosion, normal development and male-female pairing on the same day are selected and put into a cage for oviposition, and cotton dipped with a proper amount of honey water (10 percent) is hung on branches to be used as an adult insect nutrient source. Total egg production on both shoots was recorded daily until all female moths no longer lay eggs. After checking the number of eggs laid each time, the fresh branches are replaced and sprayed with 3, 5-dimethoxytoluene solution and absolute ethyl alcohol. Room temperature, shading and air humidity of 75 percent. And counting the total egg mass number and the total egg yield. Repeat 5 times, SPSS 17.0 for statistical analysis. The other three 3, 5-dimethoxytoluene solutions (2.0, 10.0, 50.0 g/L) were tested in the same manner. The results are shown in FIG. 4.
The results in FIG. 4 show that 3, 5-dimethoxytoluene has a very significant attraction effect on the selection of spodoptera exigua oviposition, both from the total egg production (FIG. 4A) and from the total egg mass number (FIG. 4B). The attracting effect is best when the mass concentration is 50.0 g/L. The maximum attraction rate was 63.2% based on the total egg laying amount in FIG. 4. As can be seen from FIG. 4, the test results at the two concentrations of 10.0 and 50.0g/L are not very different. Through analysis of test results of an EAG and a Y-type olfactometer, when the 3, 5-dimethoxytoluene is used as the beet armyworm imago attractant, the optimal use concentration is 10.0g/L, and the economic and practical effects can be achieved.
Example 5
Experiment in the field
The field verification experiment of the luring activity of 3, 5-dimethoxytoluene on the beet armyworm oviposition is carried out in a greenhouse of a rose planting base in the tribute area of Kunming city in Yunnan province. No pesticide is used within 14 days before the experiment is started, and normal water and fertilizer management is carried out. The average plant height of China rose is 20 cm at the beginning of the experiment, and the areas of the treatment cell and the control cell are 4 m each2At intervals of 2 m. Wherein, 10.0 is used for each plant in the treatment district1 mL of 3, 5-dimethoxytoluene-ethanol solution of g/L is sprayed on the whole Chinese rose plant, and the ethanol with the same volume is only sprayed on the control cell in the same method. Putting 15 artificially-fed adult beet armyworm pairs of male and female in a greenhouse, collecting and counting the egg quantity and egg mass number of the adult beet armyworm pairs every day, and counting the total egg production quantity and total egg mass number after 5 days. Let 3 replicates. The same method is used to test the solution containing Tween-80 (3, 5-Dimethoxytoluene-ethanol-Tween 80 solution (3, 5-Dimethoxytoluene-ethanol-Tween 80), diluted with distilled water). The humidity in the greenhouse is 75-95%, the temperature is 18-40 ℃, and other methods and conditions are the same as those of an indoor egg laying amount test experiment. No work such as soil and plant sterilization was required, and the results are shown in Table 2.
TABLE 23, 5-Dimethoxytoluene field experiments
Table 2 Field experiment for 3, 5-dimethoxytoluene
aAll four data were calculated from the ethanol control.
As can be seen from the total values of the number of egg masses and the egg laying amount in Table 2, under the actual production conditions of rose planting in the greenhouse, 3, 5-dimethoxytoluene also shows an obvious attraction effect on egg laying of beet armyworm. From the aspect of egg laying amount, the attraction rate can reach 51.2 percent. The effect of 3, 5-dimethoxytoluene on attracting spodoptera exigua female moths is verified in field experiments. Tween-80 is added into the attractant, and no obvious influence is found from the insect attracting effect, but the solubility of the 3, 5-dimethoxytoluene in water is increased, so that ethanol can be replaced by water in the actual using process.
In conclusion, the 3, 5-dimethoxytoluene can effectively play a role in regulating and controlling the selection behavior of the beet armyworm egg-laying place, plays a key role in the selection process of the beet armyworm egg-laying place, and can be used for pollution-free control of the beet armyworm and other pests, and has wide application prospects.
Claims (8)
1. The application of the 3, 5-dimethoxytoluene is characterized in that the 3, 5-dimethoxytoluene is applied to the preparation of a pest attractant, and the pests are spodoptera exigua female moths.
2. The use of 3, 5-dimethoxytoluene as claimed in claim 1, wherein the effective concentration of 3, 5-dimethoxytoluene in the pest attractant is 0.4-50.0 g/L.
3. The use of 3, 5-dimethoxytoluene according to claim 1 or 2, characterized in that the effective concentration of 3, 5-dimethoxytoluene in the pest attractant is 10.0 g/L.
4. The application of 3, 5-dimethoxytoluene as claimed in claim 1, wherein the preparation method of the pest attractant comprises the steps of preparation of mother liquor A, preparation of auxiliary liquor B and preparation, and specifically comprises the following steps:
A. preparing a mother solution A: accurately weighing 3, 5-dimethoxytoluene, adding the 3, 5-dimethoxytoluene into a solvent for dissolving to prepare mother liquor A;
B. preparing an auxiliary liquid B: preparing a tween-80 solution by using deionized water to obtain an auxiliary solution B;
C. preparation: and mixing the mother solution A and the auxiliary solution B, carrying out ultrasonic treatment, and then fixing the volume to obtain the target pest attractant.
5. The use of 3, 5-dimethoxytoluene according to claim 4, characterized in that the solvent in step A is one or more of ethanol, n-hexane, methanol and dichloromethane.
6. The use of 3, 5-dimethoxytoluene according to claim 4, characterized in that the concentration of Tween-80 in step B is 0.1% by mass.
7. The use of 3, 5-dimethoxytoluene according to claim 4, wherein the ultrasonic treatment in step C is ultrasonic treatment at an ultrasonic frequency of 40-60 KHz for 4-6 min.
8. The application of 3, 5-dimethoxytoluene as claimed in claim 1, wherein the usage method of the pest attractant is that the pest attractant is diluted by 0-125 times with ethanol or water and then directly and uniformly sprayed on flower buds or branches and leaves of plants.
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| Yu Cao等.Laboratory and field investigation on the orientation of Frankliniella occidentalis (Thysanoptera: Thripidae) to more suitable host plants driven by volatiles and component analysis of volatiles.《Pest Manag Sci》.2018,第75卷(第3期),598-606. * |
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