JP6659440B2 - Sex attractant of the scorpion scale - Google Patents
Sex attractant of the scorpion scale Download PDFInfo
- Publication number
- JP6659440B2 JP6659440B2 JP2016087422A JP2016087422A JP6659440B2 JP 6659440 B2 JP6659440 B2 JP 6659440B2 JP 2016087422 A JP2016087422 A JP 2016087422A JP 2016087422 A JP2016087422 A JP 2016087422A JP 6659440 B2 JP6659440 B2 JP 6659440B2
- Authority
- JP
- Japan
- Prior art keywords
- sex
- composition
- species
- sex attractant
- attractant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000877 Sex Attractant Substances 0.000 title claims description 113
- 241000239226 Scorpiones Species 0.000 title claims description 19
- 239000000203 mixture Substances 0.000 claims description 113
- 241000238631 Hexapoda Species 0.000 claims description 88
- 238000000034 method Methods 0.000 claims description 68
- 238000004891 communication Methods 0.000 claims description 42
- 239000000126 substance Substances 0.000 claims description 41
- 241000131091 Lucanus cervus Species 0.000 claims description 27
- 239000004480 active ingredient Substances 0.000 claims description 25
- 238000013268 sustained release Methods 0.000 claims description 15
- 239000012730 sustained-release form Substances 0.000 claims description 15
- 150000001875 compounds Chemical class 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 claims description 12
- 241000207929 Scutellaria Species 0.000 claims description 6
- 241000131077 Lucanidae Species 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 4
- 241000237858 Gastropoda Species 0.000 claims 2
- 241000894007 species Species 0.000 description 83
- 241000607479 Yersinia pestis Species 0.000 description 47
- 241001508691 Martes zibellina Species 0.000 description 40
- -1 ester compound Chemical class 0.000 description 27
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 24
- 238000009434 installation Methods 0.000 description 23
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 19
- 239000007788 liquid Substances 0.000 description 19
- 238000009826 distribution Methods 0.000 description 18
- 241000258937 Hemiptera Species 0.000 description 14
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 12
- 230000001939 inductive effect Effects 0.000 description 12
- 239000000463 material Substances 0.000 description 12
- 239000000853 adhesive Substances 0.000 description 11
- 230000001070 adhesive effect Effects 0.000 description 11
- 239000005667 attractant Substances 0.000 description 11
- 230000031902 chemoattractant activity Effects 0.000 description 11
- 230000000694 effects Effects 0.000 description 11
- 239000000575 pesticide Substances 0.000 description 11
- 239000002904 solvent Substances 0.000 description 11
- 239000002552 dosage form Substances 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 9
- 239000003795 chemical substances by application Substances 0.000 description 9
- 239000000843 powder Substances 0.000 description 9
- 230000006399 behavior Effects 0.000 description 8
- 230000013011 mating Effects 0.000 description 8
- 239000000546 pharmaceutical excipient Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 239000008187 granular material Substances 0.000 description 6
- 239000000123 paper Substances 0.000 description 6
- 229920003023 plastic Polymers 0.000 description 6
- 239000004033 plastic Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 238000000576 coating method Methods 0.000 description 5
- 229910052500 inorganic mineral Inorganic materials 0.000 description 5
- 239000011707 mineral Substances 0.000 description 5
- VHYFNPMBLIVWCW-UHFFFAOYSA-N 4-Dimethylaminopyridine Chemical compound CN(C)C1=CC=NC=C1 VHYFNPMBLIVWCW-UHFFFAOYSA-N 0.000 description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 4
- 244000299507 Gossypium hirsutum Species 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 239000000287 crude extract Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000002917 insecticide Substances 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 239000003960 organic solvent Substances 0.000 description 4
- 229920000151 polyglycol Polymers 0.000 description 4
- 239000010695 polyglycol Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241001465977 Coccoidea Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241001415279 Pseudococcidae Species 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 229920001971 elastomer Polymers 0.000 description 3
- 239000003995 emulsifying agent Substances 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 239000005060 rubber Substances 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- BDNKZNFMNDZQMI-UHFFFAOYSA-N 1,3-diisopropylcarbodiimide Chemical compound CC(C)N=C=NC(C)C BDNKZNFMNDZQMI-UHFFFAOYSA-N 0.000 description 2
- YYPNJNDODFVZLE-UHFFFAOYSA-N 3-methylbut-2-enoic acid Chemical compound CC(C)=CC(O)=O YYPNJNDODFVZLE-UHFFFAOYSA-N 0.000 description 2
- 241000254173 Coleoptera Species 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 241000218922 Magnoliophyta Species 0.000 description 2
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 2
- 238000005481 NMR spectroscopy Methods 0.000 description 2
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 2
- 241000209270 Phenacoccus solenopsis Species 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 239000005941 Thiamethoxam Substances 0.000 description 2
- 241001125843 Trichiuridae Species 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 150000008055 alkyl aryl sulfonates Chemical class 0.000 description 2
- 229940045714 alkyl sulfonate alkylating agent Drugs 0.000 description 2
- 150000008052 alkyl sulfonates Chemical class 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000012298 atmosphere Substances 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 2
- 239000003610 charcoal Substances 0.000 description 2
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000001627 detrimental effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- BGRWYRAHAFMIBJ-UHFFFAOYSA-N diisopropylcarbodiimide Natural products CC(C)NC(=O)NC(C)C BGRWYRAHAFMIBJ-UHFFFAOYSA-N 0.000 description 2
- 239000002270 dispersing agent Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 150000002191 fatty alcohols Chemical class 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000009931 harmful effect Effects 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000001819 mass spectrum Methods 0.000 description 2
- 230000007758 mating behavior Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 2
- 229920000609 methyl cellulose Polymers 0.000 description 2
- 239000001923 methylcellulose Substances 0.000 description 2
- PSZYNBSKGUBXEH-UHFFFAOYSA-N naphthalene-1-sulfonic acid Chemical compound C1=CC=C2C(S(=O)(=O)O)=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-N 0.000 description 2
- 150000002790 naphthalenes Chemical class 0.000 description 2
- 230000003071 parasitic effect Effects 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 210000004894 snout Anatomy 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
- NWWZPOKUUAIXIW-FLIBITNWSA-N thiamethoxam Chemical compound [O-][N+](=O)\N=C/1N(C)COCN\1CC1=CN=C(Cl)S1 NWWZPOKUUAIXIW-FLIBITNWSA-N 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 2
- 229920002554 vinyl polymer Polymers 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- WHOZNOZYMBRCBL-OUKQBFOZSA-N (2E)-2-Tetradecenal Chemical compound CCCCCCCCCCC\C=C\C=O WHOZNOZYMBRCBL-OUKQBFOZSA-N 0.000 description 1
- WCXDHFDTOYPNIE-RIYZIHGNSA-N (E)-acetamiprid Chemical compound N#C/N=C(\C)N(C)CC1=CC=C(Cl)N=C1 WCXDHFDTOYPNIE-RIYZIHGNSA-N 0.000 description 1
- 238000005160 1H NMR spectroscopy Methods 0.000 description 1
- FOGYNLXERPKEGN-UHFFFAOYSA-N 3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfopropyl)phenoxy]propane-1-sulfonic acid Chemical compound COC1=CC=CC(CC(CS(O)(=O)=O)OC=2C(=CC(CCCS(O)(=O)=O)=CC=2)OC)=C1O FOGYNLXERPKEGN-UHFFFAOYSA-N 0.000 description 1
- 240000004507 Abelmoschus esculentus Species 0.000 description 1
- 239000005875 Acetamiprid Substances 0.000 description 1
- 241000351040 Allotropa <wasp> Species 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 239000005885 Buprofezin Substances 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- 241000255749 Coccinellidae Species 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical class OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 206010014405 Electrocution Diseases 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- PNVJTZOFSHSLTO-UHFFFAOYSA-N Fenthion Chemical compound COP(=S)(OC)OC1=CC=C(SC)C(C)=C1 PNVJTZOFSHSLTO-UHFFFAOYSA-N 0.000 description 1
- 241000259121 Ferrisia virgata Species 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 244000043261 Hevea brasiliensis Species 0.000 description 1
- 241001058150 Icerya purchasi Species 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 241000500891 Insecta Species 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- IGFHQQFPSIBGKE-UHFFFAOYSA-N Nonylphenol Natural products CCCCCCCCCC1=CC=C(O)C=C1 IGFHQQFPSIBGKE-UHFFFAOYSA-N 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000596518 Planococcus kraunhiae Species 0.000 description 1
- 239000004721 Polyphenylene oxide Substances 0.000 description 1
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 1
- 241001392790 Rodolia cardinalis Species 0.000 description 1
- 241000254062 Scarabaeidae Species 0.000 description 1
- 241000053207 Scutellidae Species 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- JZFICWYCTCCINF-UHFFFAOYSA-N Thiadiazin Chemical compound S=C1SC(C)NC(C)N1CCN1C(=S)SC(C)NC1C JZFICWYCTCCINF-UHFFFAOYSA-N 0.000 description 1
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical group ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 description 1
- 240000006677 Vicia faba Species 0.000 description 1
- 235000010749 Vicia faba Nutrition 0.000 description 1
- 235000002098 Vicia faba var. major Nutrition 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- DHKHKXVYLBGOIT-UHFFFAOYSA-N acetaldehyde Diethyl Acetal Natural products CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 125000002877 alkyl aryl group Chemical group 0.000 description 1
- 150000005215 alkyl ethers Chemical class 0.000 description 1
- 125000005037 alkyl phenyl group Chemical group 0.000 description 1
- 150000008051 alkyl sulfates Chemical class 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- PRLVTUNWOQKEAI-VKAVYKQESA-N buprofezin Chemical compound O=C1N(C(C)C)\C(=N\C(C)(C)C)SCN1C1=CC=CC=C1 PRLVTUNWOQKEAI-VKAVYKQESA-N 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000000073 carbamate insecticide Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 235000005607 chanvre indien Nutrition 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000006482 condensation reaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 125000001995 cyclobutyl group Chemical group [H]C1([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- YKBZOVFACRVRJN-UHFFFAOYSA-N dinotefuran Chemical compound [O-][N+](=O)\N=C(/NC)NCC1CCOC1 YKBZOVFACRVRJN-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical compound CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000008029 eradication Effects 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 239000005038 ethylene vinyl acetate Substances 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 238000004896 high resolution mass spectrometry Methods 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- SDMSCIWHRZJSRN-UHFFFAOYSA-N isoxathion Chemical compound O1N=C(OP(=S)(OCC)OCC)C=C1C1=CC=CC=C1 SDMSCIWHRZJSRN-UHFFFAOYSA-N 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- MEBQXILRKZHVCX-UHFFFAOYSA-N methidathion Chemical compound COC1=NN(CSP(=S)(OC)OC)C(=O)S1 MEBQXILRKZHVCX-UHFFFAOYSA-N 0.000 description 1
- FZIBCCGGICGWBP-UHFFFAOYSA-N methyl 3-methylbut-2-enoate Chemical compound COC(=O)C=C(C)C FZIBCCGGICGWBP-UHFFFAOYSA-N 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229920003052 natural elastomer Polymers 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 229920001194 natural rubber Polymers 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- SNQQPOLDUKLAAF-UHFFFAOYSA-N nonylphenol Chemical compound CCCCCCCCCC1=CC=CC=C1O SNQQPOLDUKLAAF-UHFFFAOYSA-N 0.000 description 1
- 229920002114 octoxynol-9 Polymers 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 229940044654 phenolsulfonic acid Drugs 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- MIOBBYRMXGNORL-UHFFFAOYSA-N pyrifluquinazon Chemical compound C1C2=CC(C(F)(C(F)(F)F)C(F)(F)F)=CC=C2N(C(=O)C)C(=O)N1NCC1=CC=CN=C1 MIOBBYRMXGNORL-UHFFFAOYSA-N 0.000 description 1
- JWVCLYRUEFBMGU-UHFFFAOYSA-N quinazoline Chemical compound N1=CN=CC2=CC=CC=C21 JWVCLYRUEFBMGU-UHFFFAOYSA-N 0.000 description 1
- 230000010539 reproductive behavior Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 150000004760 silicates Chemical class 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000004071 soot Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 229920003051 synthetic elastomer Polymers 0.000 description 1
- 239000005061 synthetic rubber Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000001926 trapping method Methods 0.000 description 1
- UBOXGVDOUJQMTN-UHFFFAOYSA-N trichloroethylene Natural products ClCC(Cl)Cl UBOXGVDOUJQMTN-UHFFFAOYSA-N 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 244000000006 viral plant pathogen Species 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 239000003021 water soluble solvent Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Description
本発明はクロテンコナカイガラムシの性誘引物質、その製造方法又はそれを有効成分として含む性誘引組成物、その性誘引組成物を備えた捕虫装置又は交信撹乱装置、及びそれらの装置を用いたクロテンコナカイガラムシの防除法に関するものである。 The present invention relates to a sex attractant substance for a scorpion locust, a method for producing the same or a sex attractant composition containing the same as an active ingredient, an insect trapping device or a communication disrupting device equipped with the sex attractant composition, and a scutellina using such a device. It is related to the control method for scale insects.
クロテンコナカイガラムシ（Phenacoccus solenopsis）（本明細書では、しばしば「本種」と表記する）は、カメムシ目（Hemiptera）カイガラムシ上科（Coccoidea）コナカイガラムシ科（Pseudococcidae）に属する小型の難防除害虫である。本種の2齢以降の幼虫及びメス成虫は、楕円形で、自ら分泌した白色のロウ質物で背面を被覆している。幼虫及びメス成虫は草食性で、口吻を被子植物組織に差し込み、篩管液等を摂取することから、植物の成長阻害、排泄物によって誘発されるすす病、及び植物病原ウイルスの媒介の原因害虫となり得る。 The stag beetle (Phenacoccus solenopsis) (often referred to herein as the "species") is a small, difficult-to-control pest belonging to the order Hemiptera, Coccoidea, and Pseudococcidae. . Larvae and adult females of the second and subsequent instars of this species are elliptical and cover the back with their own secreted white waxy material. The larvae and female adults are herbivorous, and because they insert the snout into the angiosperm tissue and ingest phloem sap, etc., they inhibit plant growth, soot disease induced by excreta, and pests that cause plant pathogenic virus transmission. Can be
日本において本種は近年になって確認されたことから、外国から持ち込まれた侵入害虫と考えられている。現時点での国内分布は局所的であるが、他国ではマンゴー等の果樹やトマト等の野菜類、又は綿花等の農作物に甚大な被害を及ぼしており（非特許文献１）、農産業分野における経済的損失の原因となっている。そのため、国内分布の拡大阻止、再侵入予防、及び適切な防除対策が急務とされている。この課題を解決するためには、国内における本種の発生又は分布状況の把握や効率的な防除方法の確立が必要である。 This species has recently been identified in Japan and is considered an invasive pest brought in from abroad. At present, domestic distribution is local, but in other countries, fruit trees such as mango, vegetables such as tomatoes, and crops such as cotton are seriously damaged (Non-Patent Document 1), and the economy in the agricultural industry field Cause loss of quality. Therefore, it is urgently necessary to prevent the spread of domestic distribution, prevent re-entry, and take appropriate control measures. In order to solve this problem, it is necessary to grasp the occurrence or distribution of this species in Japan and establish an efficient control method.
一般にカイガラムシ上科昆虫の防除には化学農薬が使用される。例えば、アセタミプリド、チアメトキサム、ジノテフラン及びチアメトキサム等を有効成分とするネオニコチノイド系殺虫剤、メダチオン（DMTP）及びイソキサチオン等を有効成分とする有機リン系殺虫剤、ブプロフェジン等を有効成分とするチアジアジン系殺虫剤、ピリフルキナゾン等を有効成分とするキナゾリン系殺虫剤、及びアラニカルブ等を有効成分とするカーバメート系殺虫剤等が挙げられる。しかし、化学農薬を用いた防除法は、薬剤抵抗性個体の出現、リサージェンス（農薬使用による天敵や競合種の除去を原因とする標的害虫の再増加現象）、環境汚染、及び農作物への残留等が大きな問題となっている。また、2齢以降のコナカイガラムシは、ロウ質物からなる殻で被覆されているため、通常の化学農薬が効きにくいという問題もある。 Generally, chemical pesticides are used for controlling insects of the scale insects. For example, neonicotinoid insecticides containing acetamiprid, thiamethoxam, dinotefuran and thiamethoxam as active ingredients, organophosphorus insecticides containing medathion (DMTP) and isoxathion as active ingredients, and thiadiazine insecticides containing buprofezin and the like as active ingredients. And quinazoline insecticides containing pyrifluquinazone as an active ingredient and carbamate insecticides containing alanicarb as an active ingredient. However, the control method using chemical pesticides involves the emergence of drug-resistant individuals, resurgence (re-enhancement of target pests due to removal of natural enemies and competing species by using pesticides), environmental pollution, and residue on agricultural products. Is a major problem. In addition, since the insects of the second year of age are covered with a shell made of a waxy substance, there is also a problem that ordinary chemical pesticides are hardly effective.
近年では、環境に配慮する関心の高まりから環境と調和した持続的な防除技術への移行が求められており、化学農薬の代替防除技術として生物防除が注目されている。生物防除は、自然生態系における捕食被食関係や宿主寄生体関係に基づき、天敵を生物農薬（天敵製剤）に利用して害虫、病原性微生物又は雑草等を防除する方法である。コナカイガラムシ科昆虫においても、例えば、日本土着種のフジコナカイガラムシ（Planococcus kraunhiae）に対しては、土着寄生蜂であるフジコナカイガラクロバチ（Allotropa subclavata）を利用して防除する方法等が開発されている（非特許文献２）。しかし、一般に寄生種は、宿主特異性が極めて高く、同属種であっても捕食又は寄生対象とならないことが多い。また、クロテンコナカイガラムシは侵入種であり、土着種ではないことから、本種を特異的に捕食又は寄生する天敵種が日本国内には分布していない可能性も高い。少なくとも現時点でクロテンコナカイガラムシに対する有効な天敵種は日本国内では知られていない。一方、外国産の天敵種を国内に導入し、放飼することによって標的害虫を駆除する方法もある。ワタフキカイガラムシ（Icerya purchasi）の天敵であるオーストラリア原産種ベダリアテントウ（Rodolia cardinalis）の導入例は、その好例である。しかし、本種の天敵種を利用した生物防除法は確立しておらず、開発及び導入後の在来種への影響調査等には長期期間を要し、その実用化は容易ではない。 In recent years, there has been a demand for a shift to sustainable pest control technology in harmony with the environment due to increasing interest in consideration of the environment, and biological control has attracted attention as an alternative control technique for chemical pesticides. Biological control is a method for controlling pests, pathogenic microorganisms or weeds by using natural enemies as biological pesticides (natural enemy preparations) based on the relationship between predators and prey and host parasites in natural ecosystems. In the insects of the family Pseudococcidae, for example, a method of controlling Japanese indigenous species, Fujiconidae (Planococcus kraunhiae), using the indigenous parasitic bee, Fujinokaiarabachi (Allotropa subclavata), has been developed. (Non-Patent Document 2). However, in general, parasitic species have extremely high host specificity, and even if they are of the same genus, they are not often targets for predation or parasitism. In addition, since the stag beetle is an invasive species and not an indigenous species, it is highly likely that no natural enemy species that specifically prey or parasitize this species is distributed in Japan. At least at present, no effective natural enemy species against the stag beetle are known in Japan. On the other hand, there is a method to control the target pests by introducing foreign natural enemy species into the country and releasing them. A good example is the introduction of the Australian native species Vedalia ladybird (Rodolia cardinalis), a natural enemy of the cotton beetle (Icerya purchasi). However, a biological control method using this natural enemy species has not been established, and it takes a long period of time to investigate the effects on native species after development and introduction, and its practical application is not easy.
一方、性誘引物質を利用した防除方法が様々な害虫で実用化されている。性誘引物質とは、成熟生物個体（成体）が分泌する化学物質で、同種の異性成体に対して強力な誘引作用を示す。それ故、人工的に化学合成した性誘引物質を用いて標的害虫の性誘引物質を人為的に操作することにより、標的害虫を効率的に防除することができる。例えば、圃場等の対象エリアに標的害虫の性誘引物質を散布することで、標的害虫のオス個体のメス探索行動を阻害して交尾機会を剥奪することができる。この方法を数世代に亘って繰り返すことで、対象エリアから標的害虫を駆除することが可能となる。このような性誘引物質を有効成分とする製剤は、交信撹乱剤と呼ばれている。カイガラムシ上科昆虫のオス成虫の形態は、メス成虫のそれと全く異なり、翅を有し、性誘引物質に誘引されて自由に移動することができる。一方で、オス成虫の寿命は数日以下であり、ごく限られた期間に交尾する。したがって、交信撹乱剤は、カイガラムシ上科の管理技術としても有用である（非特許文献３）。性誘引物質を利用した防除方法は、化学農薬の防除方法のような直接的な殺虫作用はないものの、他の多くの点で化学農薬よりも優れている。例えば、性誘引物質自体が昆虫由来の天然物質であるため環境汚染問題が生じない、繁殖上不可欠な物質であることから抵抗性個体も出現しない、そして種特異的に効果を示すのでリサージェンスの問題も発生することがない、と言った利点を有する。 On the other hand, control methods using sex attractants have been put to practical use for various pests. A sex attractant is a chemical substance secreted by a mature organism (adult), and has a strong attracting action to the same type of isomer. Therefore, by artificially manipulating the sex attractant of the target pest using the artificially chemically synthesized sex attractant, the target pest can be efficiently controlled. For example, by spraying a sex attractant of the target pest on a target area such as a field, it is possible to obstruct the female searching behavior of the male individual of the target pest and thereby deprive the opportunity of mating. By repeating this method for several generations, it becomes possible to exterminate the target pest from the target area. A preparation containing such a sex attractant as an active ingredient is called a communication disruptor. The male adult form of the scale insects is completely different from that of female adults and has wings and can freely move by being attracted by sex attractants. On the other hand, the lifespan of male adults is less than a few days, and they copulate for a very limited period. Therefore, the communication disruption agent is also useful as a management technique for the scale insects of the scale insects (Non-Patent Document 3). Although the control method using a sex attractant does not have a direct insecticidal effect unlike the control method for chemical pesticides, it is superior in many other respects to chemical pesticides. For example, the sex attractant itself is a natural substance derived from insects, so there is no problem of environmental pollution.Because it is an essential substance for reproduction, no resistant individuals appear.Resurgence is a problem because it shows species-specific effects. Also does not occur.
また、性誘引物質は、防除対象種の分布及び発生状況の調査においても極めて有効である。例えば、性誘引物質の種特異的誘引作用を利用することで、対象種のみを捕えて、その分布及び発生状況をモニタリングすることで防除計画を立案できる。このような作用は、化学農薬や生物防除にはない特徴である。 Sex attractants are also very effective in investigating the distribution and occurrence of species to be controlled. For example, by utilizing the species-specific attraction of a sex attractant, a control plan can be formulated by capturing only the target species and monitoring the distribution and occurrence thereof. Such an action is a feature not found in chemical pesticides or biological control.
したがって、性誘引物質の化学構造を明らかにし、工業的に合成することができれば、交信撹乱剤の有効成分として、また標的害虫の発生調査手段として活用できる。実際、多くの害虫種で性誘引物質が同定され、その化学構造に基づいて化学合成された性誘引物質がトラップ等の資材として害虫管理に利用されている（特許文献１及び２）。 Therefore, if the chemical structure of a sex attractant can be clarified and synthesized industrially, it can be used as an active ingredient of a communication disrupting agent and as a means of investigating the occurrence of target pests. In fact, sex attractants have been identified in many pest species, and sex attractants chemically synthesized based on their chemical structures are used in pest management as materials such as traps (Patent Documents 1 and 2).
しかし、性誘引物質は、極めて高い種特異性を有するが故に、種ごとにその化学構造が全く異なる。そのため適用対象の汎用性がほとんどなく、標的害虫ごとに単離し、同定しなければならない。また、メス個体から分泌される性誘引物質は極めて微量であり、その単離、回収や化学構造の同定も容易ではない。それ故に、クロテンコナカイガラムシの性誘引物質についても、これまで単離された報告はなく、またその化学構造についても未知のままである。 However, since sex attractants have extremely high species specificity, their chemical structures are completely different from species to species. Therefore, there is little versatility of the application object, and it must be isolated and identified for each target pest. In addition, the sex attractant secreted from the female individual is extremely small, and its isolation, recovery, and identification of the chemical structure are not easy. Therefore, there has been no report on the sex attractant of the scorpion locust, so far, and its chemical structure remains unknown.
本発明は、クロテンコナカイガラムシの分布及び発生調査並びに防除を目的とした性誘引物質、それを製造する方法、並びにその性誘引物質を有効成分とする農業害虫管理資材としての組成物を開発し、提供することを課題とする。 The present invention is a sex attractant for distribution and outbreak investigation and control of the stag beetle, a method for producing the same, and a composition as an agricultural pest management material containing the sex attractant as an active ingredient, The task is to provide.
本発明者は、上記課題を解決するために、クロテンコナカイガラムシの性誘引物質（本明細書では、しばしば「クロテン用性誘引物質」と略称する）の単離を試み、膨大な数の本種未交尾メスから性誘引物質を単離・同定することに成功した。同定された性誘引物質は、新規のエステル化合物であり、その化学構造に基づいて性誘引物質を合成する方法も開発した。本発明は、上記知見や開発結果に基づくものであり、すなわち以下を提供する。
（１）以下の式（I）で示される化合物。
（３）前記（２）に記載の性誘引物質を有効成分として含むクロテンコナカイガラムシの性誘引組成物。
（４）前記（３）に記載の性誘引組成物、担体、及び捕虫器を備えたクロテンコナカイガラムシの捕虫装置。
（５）前記担体が徐放性担体である、前記（４）に記載の捕虫装置。
（６）前記（３）に記載の性誘引組成物、及び担体を備えたクロテンコナカイガラムシの交信撹乱装置。
（７）前記担体が徐放性担体である、前記（６）に記載の交信撹乱装置。
（８）前記（４）又は（５）に記載の捕虫装置、又は前記（６）又は（７）に記載の交信攪乱装置を用いるクロテンコナカイガラムシの防除方法。
（９）以下の式（II）で示される化合物
前記（１）の式（I）で示される化合物からなるクロテン用性誘引物質を製造する方法。
In order to solve the above-mentioned problems, the present inventors have attempted to isolate a sex attractant for scorpion locust (sometimes abbreviated as “sex attractant for sable” in the present specification), We succeeded in isolating and identifying sex attractants from unmated females. The identified sex attractant is a novel ester compound, and a method for synthesizing the sex attractant based on its chemical structure was also developed. The present invention is based on the above findings and development results, that is, provides the following.
(1) A compound represented by the following formula (I).
(3) A sex attractant composition of the scorpion insect, which contains the sex attractant described in (2) as an active ingredient.
(4) An insect trapping apparatus for the black stag beetle, comprising the sex attraction composition according to (3), a carrier, and a trap.
(5) The insect trap according to (4), wherein the carrier is a sustained-release carrier.
(6) An apparatus for disrupting communication of stag beetle, comprising the sex-inducing composition according to (3) and a carrier.
(7) The communication disruption device according to (6), wherein the carrier is a sustained-release carrier.
(8) A method for controlling stag beetles using the insect trap according to (4) or (5) or the communication disruption device according to (6) or (7).
(9) Compound represented by the following formula (II)
本発明のクロテン用性誘引物質によれば、本種オス成虫を種特異的に誘引することができる。また、その誘引作用を利用して、対象エリア内の本種の分布状況及び／又は発生状況をモニタリングしたり、本種オス成虫を特異的に誘殺することができる。 ADVANTAGE OF THE INVENTION According to the sex attractant for sable of the present invention, it is possible to species-specifically attract the adult male of this species. Further, by using the attracting action, it is possible to monitor the distribution status and / or occurrence status of this species in the target area, and specifically to kill adult adults of this species.
本発明の性誘引組成物を備えた捕虫装置によれば、本種オス成虫を捕獲して、本種の分布や発生の調査、又は捕殺することができる。 ADVANTAGE OF THE INVENTION According to the insect trap apparatus provided with the sex attraction composition of this invention, the adult of this species can be caught and the distribution and generation | occurrence | production of this species can be investigated or killed.
本発明の性誘引組成物を備えた交信撹乱装置によれば、本種の交尾行動を撹乱することができる。 According to the communication disrupting device provided with the sex-inducing composition of the present invention, it is possible to disrupt the mating behavior of this species.
本発明のクロテン用性誘引物質製造方法によれば、本種の性誘引物質を化学合成法により製造することができる。 According to the method for producing a sex attractant for sables of the present invention, a sex attractant of this type can be produced by a chemical synthesis method.
１．クロテンコナカイガラムシの性誘引物質とその製造方法
１−１．概要
本発明の第１の態様は、クロテンコナカイガラムシの性誘引物質（クロテン用性誘引物質）とその製造方法である。本発明の性誘引物質は、新規エステル化合物からなり、本種オス成虫に対して種特異的誘引作用を示す。また、本発明の製造方法は、本種特異的な性誘引物質を化学合成により製造することができる。
1. Sex attractant of scutellaria and its production method 1-1. Outline A first aspect of the present invention is a sex attractant (sex attractant for sable) of stag beetle and a method for producing the same. The sex attractant of the present invention is composed of a novel ester compound, and exhibits a species-specific attracting action on the adult male of the present species. In addition, the production method of the present invention can produce a species-specific sex attractant by chemical synthesis.
１−２．定義
本明細書で頻用する用語について、以下で定義する。
「クロテンコナカイガラムシ（Phenacoccus solenopsis）」は、カメムシ目（Hemiptera）カイガラムシ上科（Coccoidea）コナカイガラムシ科（Pseudococcidae）に属する昆虫である。熱帯〜温帯を中心とした世界各地に分布し、様々な被子植物を宿主植物とするが、農作物では、特にワタ及びオクラ等のアオイ科（Malvaceae）植物、ヒマワリ等のキク科（Asteraceae）植物、ナスやトマト等のナス科（Soanaceae）植物に対して甚大な被害を及ぼす難防除農業害虫として知られている。農業害虫として重要なのは、植物の篩管液等を摂取する幼虫及びメス成虫である。オス成虫は、メス成虫と異なり一対の翅や触角を有し、長距離移動が可能であるが、口吻がなく短命であるため、直接的な農業害虫とはならない。ただし、本明細書における標的害虫は、本種のオス成虫個体である。
1-2. Definitions Terms used frequently herein are defined below.
“Phenacoccus solenopsis” is an insect belonging to the order Hemoptera, Coccoidea, and Pseudococcidae. It is distributed in various parts of the world, mainly in the tropical to temperate zones, and uses various angiosperms as host plants. In agricultural crops, in particular, Malvaceae plants such as cotton and okra, Asteraceae plants such as sunflower, It is known as a difficult-to-control agricultural pest that causes enormous damage to solanaceae plants such as eggplants and tomatoes. Important as agricultural pests are larvae and female adults that ingest plant phloem sap and the like. Male adults, unlike female adults, have a pair of wings and antennae and can move long distances, but they do not become direct agricultural pests because they have no snout and are short-lived. However, the target pest in the present specification is a male adult individual of this species.
本明細書において「性誘引物質」とは、性フェロモンとも呼ばれる生物の繁殖行動に関与する化学物質である。一般的に成体が分泌し、種特異性が非常に高く、同種の異性成体に対してのみ強力な誘引作用を示す。通常、メス成体が同種のオス成体を誘引する目的で分泌するが、その逆のケースも知られている。本発明の標的害虫であるクロテンコナカイガラムシは、メス成虫が本種のオス成虫を誘引するために性誘引物質を分泌する。本発明のクロテン用性誘引物質は、後述する式（I）で示される構造と同一の構造を有する限り、由来は問わない。例えば、本種由来であってもよいし、式（I）の化学式に基づいて化学合成された化合物であってもよい。 As used herein, the term “sex attractant” refers to a chemical substance involved in the reproductive behavior of an organism, also called a sex pheromone. It is generally secreted by adults, has a very high species specificity, and has a strong attracting effect only to the same adult isomer. Normally, female adults secrete to attract male adults of the same species, but the opposite is also known. The target insect pest of the present invention, the black-tailed scale insect, secretes a sex attractant in order for female adults to attract male adults of this species. The origin of the sex attractant for sable of the present invention is not limited as long as it has the same structure as the structure represented by the formula (I) described below. For example, it may be derived from this species or a compound chemically synthesized based on the chemical formula of formula (I).
本明細書において「農薬」とは、標的害虫に対して成長阻害、変態阻害、殺虫作用、誘引作用等の生理的影響及び／又は行動的影響を及ぼす薬剤又は組成物をいう。 As used herein, the term "pesticide" refers to a drug or a composition that exerts physiological and / or behavioral effects such as growth inhibition, metamorphosis inhibition, insecticidal action, and attractant action on a target pest.
１−３．構成
クロテン用性誘引物質は、以下の式（I）で示される（2,2-ジメチル-3-(プロパン-2-イリデン)シクロブチル)メチル3-メチルブタ-2-エノエート（（2,2-dimethyl-3-(propan-2-ylidene)cyclobutyl)methyl 3-methylbut-2-enoate）（本明細書では、しばしば慣用名「セネシオ酸マコネリル」と表記する）からなる。
1-3. The sable sex attractant is represented by the following formula (I): (2,2-dimethyl-3- (propane-2-ylidene) cyclobutyl) methyl 3-methylbut-2-enoate ((2,2-dimethyl -3- (propan-2-ylidene) cyclobutyl) methyl 3-methylbut-2-enoate) (often referred to herein as the common name "maconeryl sensioate").
セネシオ酸マコネリルは、常温（5〜35℃）で揮発性及び芳香性を有する無色の液体である。 Maconeryl sensioate is a colorless liquid that is volatile and aromatic at room temperature (5-35 ° C.).
セネシオ酸マコネリルは、クロテンコナカイガラムシ特異的な性誘引物質の構成成分として同定された新規のエステル化合物であり、本種オス成虫に対してのみ強い誘引作用を示す。したがって、本発明の性誘引物質の対象害虫は、本種オス成虫となる。 Maconeryl sensioate is a novel ester compound identified as a component of a sex attractant specific to scorpion locust, and has a strong attracting effect only to the adult male of this species. Therefore, the target pest of the sex attractant of the present invention is an adult of this species.
１−４．製造方法
クロテン用性誘引物質であるセネシオ酸マコネリルは、化学合成によって人工的に製造することができる。製造方法は、特に限定はしないが、例えば、以下の式（II）で示される公知のアルコール化合物であるマコネリオール（Zhang, A. et al., 2004, Proc. Natl. Acad. Sci. USA, 101:9601-9606）及び以下の式（III）で示される化合物であるセネシオ酸を混合し、脱水縮合反応させることによって得ることができる。
1-4. Production Method Maconeryl sensioate, which is a sex attractant for sable, can be artificially produced by chemical synthesis. Although the production method is not particularly limited, for example, maconeriol (Zhang, A. et al., 2004, Proc. Natl. Acad. Sci. USA, 101) which is a known alcohol compound represented by the following formula (II): : 9601-9606) and sensioic acid, which is a compound represented by the following formula (III), and subjected to a dehydration condensation reaction.
クロテン用性誘引物質の製造方法の具体例として、限定はしないが、ジクロロメタン中でマコネリオールとセネシオ酸を、好ましくは等量混合し、4-ジメチルアミノピリジン存在下でジイソプロピルカルボジイミドを加えてエステル化すればよい。 Specific examples of the method for producing the sable attractant include, but are not limited to, a mixture of maconeriol and sensioic acid, preferably in equal amounts, in dichloromethane and esterification by adding diisopropylcarbodiimide in the presence of 4-dimethylaminopyridine. I just need.
２．クロテンコナカイガラムシの性誘引組成物
２−１．概要
本発明の第２の態様は、クロテンコナカイガラムシの性誘引組成物（本明細書では、しばしば「性誘引組成物」と略称する）である。本発明の性誘引組成物は、前記第１態様のクロテン用性誘引物質であるセネシオ酸マコネリルを有効成分として含むことを特徴とする。本発明の性誘引組成物は、後述する本種の捕虫装置又は交信撹乱装置における誘引資材として利用することができる。
2. 2. Sexually attracting composition of scorpion scale insect 2-1. Overview A second aspect of the present invention is a sex attractant composition of the scorpion locust (herein often abbreviated as “sex attractant composition”). The sex attraction composition of the present invention is characterized by containing, as an active ingredient, maconeryl sensioate, which is the sex attraction material for sables of the first aspect. The sex-attracting composition of the present invention can be used as an attracting material in an insect trapping device or a communication disrupting device of this type described below.
２−２．構成
２−２−１．構成成分
（１）有効成分
本発明の性誘引組成物において、クロテンコナカイガラムシのオス成虫に対する誘引作用を示す有効成分は、第１態様に記載のクロテン用性誘引物質であり、上記式（I）で示されるセネシオ酸マコネリルである。
2-2. Configuration 2-2-1. Constituent (1) Active ingredient In the sex-attracting composition of the present invention, the active ingredient exhibiting the attracting action of the scorpion scale insects to the male adult is the sex-attracting substance for sable according to the first embodiment, and the formula (I) And maconeryl sensioate.
性誘引組成物に含まれる有効成分の含有量は、性誘引組成物の剤形、後述する溶媒や担体の種類、及び施用方法によって異なる。したがって、それぞれの条件を勘案して適宜定めればよい。通常は、クロテン用性誘引物質が有効成分としての機能、すなわち本種オス成虫を誘引する機能を発揮する上で必要な量であって、かつそれを適用する圃場等において、農作物等の植物や他の生物種に対して有害な作用をほとんど又は全く付与しない量をいう。性誘引組成物に含まれるクロテン用性誘引物質の具体的な含有量については、後述するクロテンコナカイガラムシの捕虫装置、及びクロテンコナカイガラムシの交信撹乱装置において説明する。 The content of the active ingredient contained in the sex attraction composition varies depending on the dosage form of the sex attraction composition, types of solvents and carriers described below, and application methods. Therefore, it may be determined appropriately in consideration of each condition. Usually, the sex attractant for sable is the amount required to exhibit the function as an active ingredient, that is, the function of attracting the adult male of this species, and in a field or the like to which it is applied, plants such as crops or the like are used. An amount that has little or no detrimental effect on other species. The specific content of the sex attractant for sable contained in the sex attractant composition will be described later in connection with a larva insect trap device for stag beetle and a communication disruption device for stag beetle.
（２）溶媒又は賦形剤
本発明の性誘引組成物は、必要に応じて農薬製剤上許容可能な溶媒又は賦形剤を含むことができる。本明細書において「農薬製剤上許容可能な溶媒又は賦形剤」とは、性誘引組成物の製剤化と施用を容易にし、有効成分であるクロテン用性誘引物質の維持又は／及び作用速度を制御する物質であって、圃場等に施用しても土壌及び水質等の環境に対する有害な影響がないか又は小さく、また農作物等の植物や他の生物種、特にヒトに対しても有害性がないか又は低いため、その農薬成分としての使用が認められている物質をいう。以下、溶媒及び賦形剤について具体的に説明をする。
(2) Solvent or excipient The sex attraction composition of the present invention can contain a solvent or excipient that is acceptable for agricultural chemicals as required. As used herein, “a solvent or excipient that is acceptable in agrochemical formulations” facilitates the formulation and application of a sex attractant composition, and maintains or / and acts on a sable attractant as an active ingredient. A substance to be controlled that has no or little harmful effects on the environment such as soil and water quality even when applied to fields, etc., and also has harmful effects on plants such as crops and other species, especially humans. A substance that is not used or is low, so its use as a pesticide ingredient is approved. Hereinafter, the solvent and the excipient will be specifically described.
溶媒には、水、又は有機溶媒が挙げられる。水には、滅菌水、脱イオン水、超純水、及び水溶液が挙げられる。有機溶媒には、ペンタン、ヘキサン、ヘプタン、ベンゼン、トルエン、キシレンクロロホルム、四塩化炭素、トリクロロエチレン、アセトン、アセトニトリル、クロロホルム、シクロヘキサノン、メタノール、エタノール、プロパノールパラフィン類、脂肪酸類、脂肪酸エステル類、又はそれらの混合溶媒等が挙げられる。中でもペンタン又はヘキサンは、本発明の性誘引組成物の溶媒として好ましい。 Solvents include water or organic solvents. Water includes sterile water, deionized water, ultrapure water, and aqueous solutions. Organic solvents include pentane, hexane, heptane, benzene, toluene, xylene chloroform, carbon tetrachloride, trichloroethylene, acetone, acetonitrile, chloroform, cyclohexanone, methanol, ethanol, propanol paraffins, fatty acids, fatty acid esters, or a mixture thereof. A mixed solvent and the like can be mentioned. Among them, pentane or hexane is preferable as the solvent of the sex attraction composition of the present invention.
本明細書において「賦形剤」とは、性誘引組成物の成形、有効成分の配合や希釈、有効成分のコーティングによる徐放化を目的として加えられる添加剤をいう。主として性誘引組成物が固形製剤の場合に使用される。賦形剤には、例えば、粉砕天然鉱物、粉砕合成鉱物、乳化剤、分散剤及び界面活性剤等が挙げられる。 As used herein, the term "excipient" refers to an additive added for the purpose of forming a sex-inducing composition, blending or diluting the active ingredient, and providing a sustained release of the active ingredient by coating. It is mainly used when the sex attraction composition is a solid preparation. Excipients include, for example, ground natural minerals, ground synthetic minerals, emulsifiers, dispersants, surfactants, and the like.
粉砕天然鉱物には、例えば、カオリン、クレイ、タルク及びチョークが挙げられる。
粉砕合成鉱物には、例えば、高分散シリカ及びシリケートが挙げられる。乳化剤としては、非イオン性乳化剤やアニオン性乳化剤（例えば、ポリオキシエチレン脂肪アルコールエーテル、アルキルスルホネート及びアリールスルホネート）が挙げられる。
Ground natural minerals include, for example, kaolin, clay, talc and chalk.
Ground synthetic minerals include, for example, highly dispersed silica and silicates. Emulsifiers include nonionic and anionic emulsifiers (eg, polyoxyethylene fatty alcohol ethers, alkyl sulfonates and aryl sulfonates).
分散剤としては、例えば、リグノ亜硫酸廃液及びメチルセルロースが挙げられる。
界面活性剤としては、例えば、リグノスルホン酸、ナフタレンスルホン酸、フェノールスルホン酸、ジブチルナフタレンスルホン酸のアルカリ金属塩、アルカリ土類金属塩及びアンモニウム塩、アルキルアリールスルホネート、アルキルスルフェート、アルキルスルホネート、脂肪アルコールスルフェート、脂肪酸及び硫酸化脂肪アルコールグリコールエーテル、さらに、スルホン化ナフタレン及びナフタレン誘導体とホルムアルデヒドの縮合物、ナフタレン又はナフタレンスルホン酸とフェノール及びホルムアルデヒドの縮合物、ポリオキシエチレンオクチルフェニルエーテル、エトキシル化イソオクチルフェノール、オクチルフェノール、ノニルフェノール、アルキルフェニルポリグリコールエーテル、トリブチルフェニルポリグリコールエーテル、トリステアリルフェニルポリグリコールエーテル、アルキルアリールポリエーテルアルコール、アルコール及び脂肪アルコール／エチレンオキシドの縮合物、エトキシル化ヒマシ油、ポリオキシエチレンアルキルエーテル、エトキシル化ポリオキシプロピレン、ラウリルアルコールポリグリコールエーテルアセタール、ソルビトールエステル、リグノ亜硫酸廃液、及びメチルセルロースが挙げられる。
Examples of the dispersant include lignosulfite waste liquid and methylcellulose.
Examples of the surfactant include lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, alkali metal salts of dibutylnaphthalenesulfonic acid, alkaline earth metal salts and ammonium salts, alkyl aryl sulfonates, alkyl sulfates, alkyl sulfonates, and fats. Alcohol sulfate, fatty acid and sulfated fatty alcohol glycol ether, condensate of sulfonated naphthalene and naphthalene derivative and formaldehyde, condensate of naphthalene or naphthalene sulfonic acid and phenol and formaldehyde, polyoxyethylene octyl phenyl ether, ethoxylated isophthalic acid Octylphenol, octylphenol, nonylphenol, alkylphenyl polyglycol ether, tributylphenyl polyglycol Ether, tristearylphenyl polyglycol ether, alkylaryl polyether alcohol, condensates of alcohol and fatty alcohol / ethylene oxide, ethoxylated castor oil, polyoxyethylene alkyl ether, ethoxylated polyoxypropylene, lauryl alcohol polyglycol ether acetal, sorbitol Esters, lignosulfite waste liquors, and methylcellulose.
２−２−２．剤形
本発明の性誘引組成物の剤形は、クロテン用性誘引物質を大気中に放出し得る形態であれば、いかなる形態であってもよく、特に限定はしない。例えば、液体状態の液剤、固体状態の固形剤とすることができる。液剤の場合、有効成分であるクロテン用性誘引物質を適当な溶液に懸濁し、必要に応じて補助剤（界面活性剤や鉱物粉等）を添加した、水溶剤、水和剤、油性分散液剤、乳剤、エマルション剤、及び油剤等が挙げられる。固形剤の場合、有効成分であるクロテン用性誘引物質を適当な賦形剤と混合して調製した、粉剤、顆粒剤、粒剤、散布剤、塗布剤、錠剤、ペースト剤、ゲル剤が挙げられる。大気中で崩壊する物質で被覆したコーティング剤も本発明の性誘引組成物の剤形に含まれる。
2-2-2. Dosage Form The dosage form of the sex attraction composition of the present invention may be in any form as long as the sex attraction substance for sable can be released into the atmosphere, and is not particularly limited. For example, a liquid agent in a liquid state or a solid agent in a solid state can be used. In the case of liquids, water-soluble solvents, wettable powders, oil-based dispersions are prepared by suspending the sable attractant as an active ingredient in an appropriate solution and adding an auxiliary agent (such as a surfactant or mineral powder) as necessary. , Emulsions, emulsions, oils and the like. In the case of solid preparations, powders, granules, granules, sprays, coatings, tablets, pastes, and gels prepared by mixing the sable attractant as an active ingredient with a suitable excipient are mentioned. Can be A coating agent coated with a substance that disintegrates in the atmosphere is also included in the dosage form of the sex attraction composition of the present invention.
２−２−３．施用方法
本発明の性誘引組成物を施用する方法は、対象エリア内に有効成分であるクロテン用性誘引物質を放出し、拡散することができれば、特に限定はしない。性誘引組成物の使用目的等に合わせて当該分野で公知の方法によって適宜施用すればよい。
2-2-3. Method of application The method of applying the sex-attracting composition of the present invention is not particularly limited as long as it can release and diffuse the sable sex-attracting substance as an active ingredient in the target area. What is necessary is just to apply suitably by the method well-known in the said field according to the use purpose etc. of a sex attraction composition.
例えば、ハウス栽培や水耕栽培のような屋内、すなわち閉鎖空間内でクロテンコナカイガラムシのオス個体に対する交信撹乱を目的として性誘引組成物を施用する場合には、性誘引組成物をその屋内に散布すればよい。この場合、性誘引組成物の剤形は、液剤又は粉剤が好ましい。液剤であれば、噴霧装置を用いて、また粉剤であればファン等を備えた拡散装置を用いて散布することができる。あるいは、後述するクロテンコナカイガラムシの交信撹乱装置を用いて、性誘引組成物をその装置内に設置して施用してもよい。この場合、性誘引組成物の剤形は、粉剤、顆粒剤、粒剤、散布剤、塗布剤、錠剤、ペースト剤、ゲル剤、又はそれらの組み合わせのいずれであってもよい。装置内に備えられた徐放性担体等に担持させるため、含浸、塗布、又は噴霧する場合であれば、液剤、粉剤、塗布剤、又はペースト剤が好ましい。 For example, in the case of indoor cultivation such as house cultivation or hydroponic cultivation, that is, when applying the sexual attraction composition for the purpose of disrupting communication with male individuals of the stag beetle in a closed space, the sexual attraction composition is sprayed indoors. do it. In this case, the dosage form of the sex attraction composition is preferably a liquid or a powder. Liquids can be sprayed using a spraying device, and powders can be sprayed using a diffusion device equipped with a fan or the like. Alternatively, the sex-attracting composition may be installed and used in a stag beetle communication disrupter described below. In this case, the dosage form of the sex attraction composition may be any of powders, granules, granules, sprays, coatings, tablets, pastes, gels, or a combination thereof. In the case of impregnation, application, or spraying for supporting on a sustained-release carrier or the like provided in the device, a liquid agent, a powder agent, a coating agent, or a paste agent is preferable.
また、閉鎖空間内又は開放空間において、本種オス成虫の誘殺を目的とする場合には、後述するクロテンコナカイガラムシの捕虫装置内に性誘引組成物を設置して施用してもよい。この場合、性誘引組成物の剤形は、粉剤、顆粒剤、粒剤、散布剤、塗布剤、錠剤、ペースト剤、ゲル剤、又はそれらの組み合わせのいずれであってもよい。 When the purpose is to attract male adults of the present species in a closed space or an open space, the sex attractant composition may be installed and applied in an insect trapping apparatus for stag beetles described below. In this case, the dosage form of the sex attraction composition may be any of powders, granules, granules, sprays, coatings, tablets, pastes, gels, or a combination thereof.
さらに、開放空間において、本種の発生状況又は分布状況の調査を目的とする場合には、クロテンコナカイガラムシの捕虫装置を用いて、上記と同様に性誘引組成物を施用すればよい。 Further, when the purpose is to investigate the occurrence or distribution of this species in an open space, the sex-attracting composition may be applied in the same manner as described above using a larvae of a larvae of the scale of Scarabaeidae.
３．クロテンコナカイガラムシの捕虫装置
３−１．概要
本発明の第３の態様は、クロテンコナカイガラムシの捕虫装置（本明細書では、しばしば「捕虫装置」と略称する）である。本発明の捕虫装置を用いることで、対象エリア内の本種の分布状況、及び発生状況を調査及び／又はモニタリングすることや、対象エリア内の本種オス成虫個体を誘殺することができる。
3. 3. Insect trapping device for stag beetle 3-1. Overview A third aspect of the present invention is a larvae of a scorpion locust insect (herein often abbreviated as “trapper”). By using the insect trap of the present invention, it is possible to investigate and / or monitor the distribution and occurrence of this species in the target area, and to kill male adults of this species in the target area.
３−２．定義
本明細書において「捕虫装置」とは、標的害虫を捕獲することを目的とした装置をいう。本明細書において「害虫」とは、農業、林業及び造園業分野に有害な影響を与える昆虫綱（Insecta）生物である。また本明細書において「標的害虫」は、捕獲対象となる害虫をいう。本明細書ではクロテンコナカイガラムシ、特にそのオス成虫個体が標的害虫に該当する。
3-2. Definitions As used herein, “insect trapping device” refers to a device intended to capture a target pest. As used herein, "pests" are Insecta organisms that have a detrimental effect on the agricultural, forestry and landscaping sectors. In this specification, “target pest” refers to a pest to be captured. In the present specification, the stag beetle, particularly an adult male, corresponds to the target pest.
本明細書において「対象エリア」とは、標的害虫の捕殺又は調査の対象となるエリアをいう。屋内のような閉鎖空間、又は屋外のような開放空間のいずれであってもよい。対象エリアの面積に限定はなく、目的に応じて適宜定められる。例えば、ビニルハウスのような閉鎖空間における標的害虫の捕殺が目的の場合には、そのビニルハウスの設置面積が対象エリアとなる。一方、圃場内における標的害虫の発生状況や分布状況の把握を目的とする場合には、その圃場が占める面積が対象エリアとなる。 In the present specification, the “target area” refers to an area to be targeted for catching or investigating a target pest. It may be either a closed space such as an indoor space or an open space such as an outdoor space. The area of the target area is not limited, and is appropriately determined according to the purpose. For example, if the purpose is to kill a target pest in a closed space such as a vinyl house, the installation area of the vinyl house is the target area. On the other hand, when the purpose is to grasp the occurrence and distribution of the target pest in the field, the area occupied by the field is the target area.
本明細書において「分布状況」とは、対象エリア内への標的害虫の侵入の有無、及び／又は対象エリア内での標的害虫の生息の有無等に関する情報をいう。 In the present specification, the “distribution state” refers to information on the presence or absence of a target pest in the target area and / or the presence or absence of the target pest in the target area.
本明細書において「発生状況」とは、対象エリア内における標的害虫の成虫発生時期、繁殖の有無、及び／又は個体数等に関する情報をいう。 As used herein, the term “occurrence status” refers to information regarding the timing of adult occurrence of target pests, the presence or absence of breeding, and / or the number of individuals in the target area.
３−３．構成
本発明の捕虫装置は、第２態様の性誘引組成物、担体、及び捕虫器を必須の構成要素として備える。以下、それぞれの構成要素について具体的に説明をする。
3-3. Configuration The insect trapping device of the present invention includes the sex attraction composition of the second aspect, a carrier, and a trap as essential components. Hereinafter, each component will be specifically described.
（１）性誘引組成物
性誘引組成物は、本発明の捕虫装置において、本種オス成虫を誘引するための重要な資材である。性誘引組成物の構成は、第２態様に記載と同一であり、ここでの説明は省略する。
(1) Sex-attracting composition The sex-attracting composition is an important material for attracting the adult male of this species in the insect trap of the present invention. The configuration of the sex attraction composition is the same as that described in the second embodiment, and a description thereof will not be repeated.
捕虫装置に用いる性誘引組成物において、クロテン用性誘引物質の含有量は、例えば、液剤の場合にはクロテン用性誘引物質を溶媒に対して0.001mg/mL〜100.0mg/mL、好ましくは0.005mg/mL〜50.0mg/mL、より好ましくは0.01mg/mL〜10.0mg/mLの濃度で含んでいればよい。溶媒は、有機溶媒、特にペンタン又はヘキサンが好適である。また、性誘引組成物を後述する徐放性担体に担持又は内蔵させて使用する場合には、担体1gあたりのクロテン用性誘引物質量が、0.005mg/g〜200mg/g、好ましくは0.01mg/g〜100mg/g又は0.02mg/g〜50.0mg/g、より好ましくは0.05mg/g〜20.0mg/gとなるように、性誘引組成物内にクロテン用性誘引物質を含んでいればよい。 In the sex attractant composition used in the insect trap, the content of the sex attractant for sable is, for example, in the case of a liquid agent, the sex attractant for sable is 0.001 mg / mL to 100.0 mg / mL to the solvent, preferably 0.005 mg / mL. It may be contained at a concentration of from mg / mL to 50.0 mg / mL, more preferably from 0.01 mg / mL to 10.0 mg / mL. The solvent is preferably an organic solvent, especially pentane or hexane. Further, when the sex attractant composition is used by being carried or incorporated in a sustained-release carrier described later, the amount of the sex attractant for sable per 1 g of the carrier is 0.005 mg / g to 200 mg / g, preferably 0.01 mg. / g to 100 mg / g or 0.02 mg / g to 50.0 mg / g, more preferably 0.05 mg / g to 20.0 mg / g, if the sex attraction composition contains a sable for sex attractant in the sex attraction composition. Good.
（２）担体
本明細書において「担体」とは、それ自身が剛性を有し、性誘引組成物を担持又は内蔵する物資である。本発明の性誘引組成物の剤形が液剤や粉剤のように不定形の場合には、それらに一定の形状を付与することができる。また剤形にかかわらず、捕虫装置において性誘引組成物の設置場所を提供でき、時として捕虫装置の筐体として機能し得る。
(2) Carrier In the present specification, the “carrier” is a material that has rigidity itself and carries or incorporates the sex attraction composition. When the dosage form of the sex-inducing composition of the present invention is irregular, such as a liquid or a powder, it can be given a certain shape. In addition, regardless of the dosage form, it is possible to provide a place for installing the sex attraction composition in the insect trapping device, and sometimes function as a casing of the insect trapping device.
担体の素材は、性誘引組成物を一定の形状で保持又は内蔵できる程度の剛性を有していれば特に限定はしない。例えば、ガラス、プラスチック（ポリエチレン、ポリプロピレン、エチレン−酢酸ビニル共重合体、ポリ塩化ビニル、ポリウレタン等を含む）、ゴム（天然ゴム、合成ゴムを含む）、セラミックス、植物由来物（綿、麻等の植物繊維、紙、木炭、木片等を含む）、動物由来物（骨片、貝殻、スポンジ、毛、羽毛、絹等を含む）、及びそれらの組み合わせが挙げられる。また、多孔質構造の素材は、比表面積が大きいため、性誘引組成物の担持量や内蔵量を増大できる。さらに、孔径の調製によって、有効成分の放出量を制御できることから、徐放性担体としても好ましい。多孔質構造の担体としては、例えば、多孔質セラミック、多孔質プラスチック、木炭、骨片、スポンジ、フィルタ（プラスチック製、植物製、動物製）、メンブレン、及び布（プラスチック製、植物製、動物製）等が挙げられる。徐放性担体には、孔径の調製が容易な多孔質プラスチック、プラスチック製メンブレン等が好適である。 The material of the carrier is not particularly limited as long as it has such a rigidity that the sex attraction composition can be held or incorporated in a certain shape. For example, glass, plastic (including polyethylene, polypropylene, ethylene-vinyl acetate copolymer, polyvinyl chloride, polyurethane, etc.), rubber (including natural rubber and synthetic rubber), ceramics, plant-derived materials (such as cotton, hemp, etc.) Plant fibers, paper, charcoal, wood chips, etc.), animal-derived materials (including bone fragments, shells, sponges, wool, feathers, silk, etc.), and combinations thereof. In addition, since the material having a porous structure has a large specific surface area, the amount of the sex-inducing composition to be carried and the amount of the built-in composition can be increased. Further, since the amount of the active ingredient released can be controlled by adjusting the pore size, it is also preferable as a sustained-release carrier. Examples of the carrier having a porous structure include porous ceramic, porous plastic, charcoal, bone fragments, sponge, filter (plastic, plant, animal), membrane, and cloth (plastic, plant, animal) ) And the like. As the sustained-release carrier, a porous plastic, a plastic membrane, or the like, whose pore size can be easily adjusted, is preferable.
担体の形状は、特に限定しない。例えば、シート状、容器状、袋状、カプセル状、管状、及びビーズ状等が挙げられる。 The shape of the carrier is not particularly limited. For example, a sheet shape, a container shape, a bag shape, a capsule shape, a tubular shape, a bead shape and the like can be mentioned.
本発明の捕虫装置における担体の具体例として、ポリエチレンからなるキャップ、ゴム、又は細管が挙げられる。 Specific examples of the carrier in the insect trap of the present invention include a cap, rubber, or a thin tube made of polyethylene.
（３）捕虫器
本発明において「捕虫器」とは、標的害虫を捕獲し、保持する器をいう。性誘引組成物によって本発明の捕虫装置に誘引されたクロテンコナカイガラムシのオス成虫は、この捕虫器によって捕獲される。したがって、捕虫器は、本発明の捕虫装置に特有の構成要素であり、性誘引組成物と並んで重要な資材である。
(3) Insect trap In the present invention, the term "insect trap" refers to a vessel that captures and holds a target pest. The male adult stag beetle attracted to the insect trap of the present invention by the sex attractant composition is captured by the insect trap. Therefore, the insect trap is a component specific to the insect trap of the present invention, and is an important material along with the sex attraction composition.
捕虫器は、標的害虫を捕獲する捕獲手段と、捕獲後の標的害虫を保持する保持手段を備えている。捕獲手段と保持手段は同一の構成であってもよいし、別々の構成であってもよい。 The trap has a capturing means for capturing the target pest and a holding means for holding the target pest after the capture. The capturing means and the holding means may have the same configuration, or may have different configurations.
同一構成の例としては、粘着トラップや水盤トラップが挙げられる。粘着トラップは、テープやシート等の支持体に粘着剤が塗布された構成を有する。性誘引組成物によって誘引された本種オス成虫は、粘着剤に捕獲され、そのまま保持されるように構成されている。この場合、粘着剤が捕獲手段と保持手段を兼ねる。また、水盤トラップは、お盆状の容器に水、水溶液、油等の液体を張った構成を有する。性誘引組成物によって誘引された本種オス成虫が液面に落下し、そのまま液体に捕獲され、そのまま保持されるように構成されている。この場合、容器に張った液体が捕獲手段と保持手段を兼ねる。本種オス成虫の誘因手段は、前述の性誘引組成物に基づくが、粘着トラップの粘着剤や水盤トラップの液体に性誘引組成物を包含させることで、粘着剤や液体が捕獲手段と保持手段に加えて、誘因手段も兼ね備えることができる。 Examples of the same configuration include an adhesive trap and a basin trap. The adhesive trap has a configuration in which an adhesive is applied to a support such as a tape or a sheet. The adult male of this species attracted by the sex attraction composition is configured to be captured by the adhesive and held as it is. In this case, the adhesive doubles as the capturing means and the holding means. The basin trap has a tray-shaped container filled with a liquid such as water, an aqueous solution, or oil. The male adult of this species attracted by the sex attraction composition falls on the liquid surface, is captured by the liquid as it is, and is held as it is. In this case, the liquid applied to the container doubles as the capturing means and the holding means. The means for inducing adult males of this species is based on the above-mentioned sex-attracting composition, but by incorporating the sex-attracting composition into the adhesive of the sticky trap and the liquid of the basin trap, the adhesive and the liquid are captured and held by the capturing means. In addition to the above, an inducing means can also be provided.
別々の構成の例としては、吸引トラップや電撃トラップが挙げられる。吸引トラップは、吸引手段（捕獲手段）と保管手段（保持手段）を有する捕虫器である。吸引手段は性誘引組成物によって捕虫装置内に誘引された本種オス成虫を、陰圧を利用して吸引し、捕獲するように構成されている。また保管手段は、捕獲した個体が逃亡できないように、又は散乱しないように所定の容器内で保管するように構成されている。電撃トラップは、電撃格子及び虫受けトレイを有する捕虫器である。捕虫装置内に設置された性誘引組成物によって本種オス成虫が誘引された際に、虫体が電撃格子に接触すると感電するように構成されている。虫受けトレイは、感電死して落下した本種オス成虫を保持するように構成されている。感電死せず、電気ショックから復活した個体が、逃亡できないように虫受けトレイに水等の液体を張ってもよい。 Examples of different configurations include a suction trap and an electric shock trap. The suction trap is an insect trap having suction means (capture means) and storage means (holding means). The suction means is configured to suck and capture the adult male of the present species attracted into the insect trapping apparatus by the sex attraction composition using negative pressure. The storage means is configured to store the captured individual in a predetermined container so as not to escape or scatter. A lightning trap is a trap that has a lightning grid and an insect receiving tray. When a male adult of this species is attracted by the sex attractant composition installed in the insect trap, the insect body is configured to receive an electric shock when it comes into contact with the electric shock grid. The insect tray is configured to hold an adult male of this species that has fallen due to electrocution. A liquid such as water may be spread on the insect receiving tray so that the individual who has recovered from the electric shock without being electrocuted cannot escape.
捕虫器は、いずれの構成であってもよく、特に、粘着トラップや水盤トラップは、簡便で低コストであるため好ましい。また2以上の構成を組み合わせてもよい。例えば、粘着トラップ又は水盤トラップとの併用が挙げられる。例えば、前述の虫受けトレイに液体を張る例は、電撃トラップと水盤トラップの併用に相当する。 The insect trap may have any configuration. In particular, the sticky trap and the basin trap are preferable because of their simplicity and low cost. Further, two or more configurations may be combined. For example, a combination with an adhesive trap or a basin trap may be used. For example, the above-described example in which the insect receiving tray is filled with liquid corresponds to a combination of an electric shock trap and a basin trap.
保持手段において、標的害虫の生死や形状維持は目的に合わせて処理される。例えば、標的害虫の捕殺が目的であれば、本種オス成虫を生存させる必要も、遺骸形状を維持する必要もない。したがって、保持手段の標的害虫はいかなる状態で保持しても構わない。一方、標的害虫の発生状況や分布状況の調査が目的であれば、生死は問わないが、データ収集上、個体数を計数できる程度に個体の形状が保持手段で維持されている必要がある。 In the holding means, the life and death of the target pest and the shape maintenance are processed according to the purpose. For example, if the purpose is to kill the target pest, there is no need for the adult male of this species to survive or to maintain the shape of the remains. Therefore, the target pest of the holding means may be held in any state. On the other hand, if the purpose is to investigate the occurrence status and distribution status of the target pest, it does not matter whether it is alive or dead. However, in data collection, the shape of the individual needs to be maintained by the holding means so that the number of individuals can be counted.
４．クロテンコナカイガラムシの交信撹乱装置
４−１．概要
本発明の第４の態様は、クロテンコナカイガラムシの交信撹乱装置（本明細書では、しばしば「撹乱装置」と略称する）である。本発明の撹乱装置を用いることで、対象エリア内の本種オス成虫のメス成虫探索行動を撹乱させて交尾機会を剥奪することができる。
4. Communication disturbance device for stag beetle 4-1. Overview A fourth aspect of the present invention is a communication disrupter (often abbreviated as “disturber” in the present specification) of a scutellid scale insect. By using the disturbance device of the present invention, it is possible to disturb the female adult search behavior of the male adult of this species in the target area, and deprive the opportunity of mating.
４−２．定義
本明細書において「交信撹乱装置」とは、標的害虫の性誘引物質を介した雌雄成虫間の交信（コミュニケーション）の撹乱又は阻害を目的とした装置をいう。
4-2. Definitions As used herein, the term “communication disrupting device” refers to a device for the purpose of disrupting or inhibiting communication (communication) between male and female adults through a sex attractant of a target pest.
本明細書において「交信撹乱」とは、性誘引物質の人工的放出により、特定の生物種における異性への誘因行動をかき乱して混乱させることをいう。本明細書においては、特に本種オス成虫によるメス成虫探索行動を、人工的に合成した同種の性誘引物質を用いて妨害し、雌雄の交尾機会を剥奪することをいう。 As used herein, the term “communication disruption” refers to disrupting and disrupting the inducing behavior to the opposite sex in a specific species by artificial release of a sex attractant. In the present specification, the term refers to interfering specifically with a male adult of this species, which is a female adult-searching behavior, using artificially synthesized same sex attractants, thereby depriving males and females of mating opportunities.
４−３．構成
本発明の交信撹乱装置は、第２態様の性誘引組成物及び担体を必須の構成要素として備える。
4-3. Configuration The communication disruption device of the present invention includes the sex attraction composition of the second aspect and a carrier as essential components.
性誘引組成物及び担体の基本構成は、第３態様の捕虫装置に記載の構成と同じである。そこで、重複する説明については省略し、ここでは交信撹乱装置に特徴的な構成についてのみを記載する。 The basic configuration of the sex attraction composition and the carrier is the same as the configuration described in the insect trap of the third embodiment. Therefore, a duplicate description will be omitted, and only a characteristic configuration of the communication disrupting apparatus will be described here.
性誘引組成物は、第２態様の性誘引組成物を用いる。交信撹乱装置に用いる性誘引組成物は、含有するクロテン用性誘引物質の量を捕虫装置の性誘引組成物におけるそれよりも高く設定することが好ましい。これは、本種に対する各装置の使用目的が異なるためである。具体的には、捕虫装置は、対象エリア内に生息する本種オス成虫を装置内に誘引し、捕獲することを目的としている。これに対して交信撹乱装置は、対象エリア内に生息する本種オス成虫の性誘引物質に対する誘引行動を撹乱させて、本種メス成虫との交尾成立を妨害又は阻止することを目的としている。一般に、対象エリア内に放出する性誘引物質量が高いほど、標的害虫の誘因行動に対する撹乱効果が高いが、逆に撹乱作用によって誘引効果は低くなってしまう。したがって、交信撹乱装置に用いる性誘引組成物は、捕虫装置の性誘引組成物よりもクロテン用性誘引物質の含有量が高い。例えば、性誘引組成物が液剤の場合にはクロテン用性誘引物質を溶媒に対して0.1mg/mL〜1000.0mg/mL、好ましくは0.5mg/mL〜500.0mg/mL、より好ましくは1.0mg/mL〜100.0mg/mLの濃度で含んでいればよい。溶媒は、捕虫装置の場合と同様、有機溶媒、特にペンタン又はヘキサンが好適である。また、性誘引組成物を後述する徐放性担体に担持又は内蔵させて使用する場合には、担体1gあたりのクロテン用性誘引物質量が、1.0mg/g〜1000.0mg/g、好ましくは2.0mg/g〜500.0mg/g、より好ましくは5.0mg/g〜200.0mg/gとなるように、性誘引組成物内にクロテン用性誘引物質を含んでいればよい。 The sex attraction composition uses the sex attraction composition of the second embodiment. It is preferable that the sex-attracting composition used in the communication disrupting device is set to contain a higher amount of the sex-attracting substance for sable than the sex-attracting composition of the insect trapping device. This is because the purpose of use of each device for this species is different. More specifically, the purpose of the insect trap device is to attract and capture the adult male species of the species living in the target area into the device. On the other hand, the communication disturbing device aims at disturbing or preventing formation of mating with a female adult of the present species by disrupting the attraction behavior of the male adult of the present species in the target area toward the sex attractant. In general, the higher the amount of the sex attractant released into the target area, the higher the disturbance effect on the inducing behavior of the target pest, but conversely, the lower the attracting effect due to the disturbance effect. Therefore, the sex attractant composition used in the communication disrupting device has a higher content of the sex attractant for sable than the sex attractant composition of the insect trap device. For example, when the sex attraction composition is a solution, the sex attraction substance for sable is 0.1 mg / mL to 1000.0 mg / mL, preferably 0.5 mg / mL to 500.0 mg / mL, more preferably 1.0 mg / mL with respect to the solvent. It may be contained at a concentration of mL to 100.0 mg / mL. The solvent is preferably an organic solvent, particularly pentane or hexane, as in the case of the insect trap. Further, when the sex attractant composition is used by being carried or incorporated in a sustained release carrier described below, the amount of the sex attractant for sable per 1 g of the carrier is 1.0 mg / g to 1000.0 mg / g, preferably 2.0 mg / g. It is sufficient that the sex-attracting composition contains the sex attractant for sable such that the concentration is from mg / g to 500.0 mg / g, more preferably from 5.0 mg / g to 200.0 mg / g.
対象害虫に対する交信撹乱は、連続的に、かつ長期間に亘って性誘引物質を放出し続けなければ効果が現れない。したがって、本装置に備えられる担体は、徐放性担体であることが望ましい。例えば、第２態様に記載の孔径を調節した多孔質担体等が挙げられる。 Communication disturbance to the target pest has no effect unless the sex attractant is continuously and continuously released for a long period of time. Therefore, the carrier provided in the present device is desirably a sustained-release carrier. For example, the porous carrier whose pore diameter is adjusted according to the second embodiment and the like can be mentioned.
５．クロテンコナカイガラムシの防除方法
５−１．概要
本発明の第５の態様は、クロテンコナカイガラムシの防除方法（本明細書では、しばしば「防除方法」と略称する）である。本発明の防除方法によれば、対象エリア内の本種を防除することができる。
5. 5. Control method of black stag beetle 5-1. Overview A fifth aspect of the present invention is a method for controlling stag beetles (hereinafter often abbreviated as “control method”). ADVANTAGE OF THE INVENTION According to the control method of this invention, this species in a target area can be controlled.
５−２．定義
本明細書において「防除」とは、標的害虫、すなわちクロテンコナカイガラムシによる害を防ぐために、その駆除、侵入防止、及び生息数の管理を行うことをいう。本明細書における「防除方法」は、本種による害を防ぐために行われる広義の防除方法であり、対象エリア内における本種の駆除方法及び発生調査方法を包含する。
5-2. Definitions As used herein, the term "control" refers to controlling, controlling the infestation, and controlling the population of a target pest, that is, the insect caused by the scale insects, scutellidae. The “control method” in the present specification is a broad control method performed to prevent harm caused by the present species, and includes a method for controlling and controlling the occurrence of the present species in a target area.
本明細書において「駆除」とは、本種を追い払い、本種の生息環境や繁殖手段を剥奪し、また本種を捕殺することによって、対象エリアから本種を排除することをいう。 As used herein, the term "exterminating" refers to removing the species from the target area by driving away the species, depriving the habitat and breeding means of the species, and killing the species.
本明細書において「発生調査」とは、対象エリア内の本種の分布状況及び発生状況を管理するために発生全般に関わる情報収集するための調査をいう。対象エリアにおける本種の生息調査（分布調査）、個体数調査、発生ステージとその時期の調査を含む。 In this specification, the term “occurrence survey” refers to a survey for collecting information on overall occurrence in order to manage the distribution status and occurrence status of this species in the target area. Includes habitat surveys (distribution surveys) of this species in the target area, population surveys, and surveys on the stage and time of occurrence.
５−３．方法
本発明の防除方法は、駆除方法及び発生調査方法に大別される。以下、それぞれの方法について具体的に説明をする。
5-3. Method The control method of the present invention is broadly classified into a control method and an occurrence investigation method. Hereinafter, each method will be specifically described.
５−３−１．駆除方法
駆除方法で、クロテンコナカイガラムシのオス成虫を捕殺して、又は繁殖手段を剥奪して、対象エリア内の本種個体数を徐々に減じることにより、最終的に対象エリアから本種を排除する、又はその個体数を著しく減じた状態で管理することを目的とする。
5-3-1. Extermination method Eradication of this species from the target area by killing the male adult of the stag beetle or removing the breeding means by the extermination method and gradually reducing the number of this species in the target area The purpose of the present invention is to control the number of individuals or to reduce the number of individuals.
駆除方法は、さらに捕殺方法と交信撹乱方法に分類できる。以下でそれぞれの方法について説明する。 Extermination methods can be further classified into killing methods and communication disruption methods. Hereinafter, each method will be described.
Ａ．捕殺方法
捕殺方法では、第３態様に記載の捕虫装置を用いて、対象エリア内の本種オス成虫を誘引し捕殺する。本方法は、対象エリアの選定工程、捕虫装置の設置工程、装置内の性誘引組成物の交換工程を含む。
A. Killing Method In the killing method, the adult male of the species in the target area is attracted and killed using the insect trapping device according to the third aspect. The method includes a step of selecting a target area, a step of installing a trapping device, and a step of exchanging the sex-attracting composition in the device.
（１）対象エリア選定工程
「対象エリア選定工程」は、クロテンコナカイガラムシを捕殺すべき対象エリアを具体的に選定する工程である。対象エリアの条件や状況が把握できなければ効率的な捕殺ができないからである。それ故に、本工程は、本方法の以降の工程における基準となる工程である。対象エリアは、特に限定はしない。実施者が希望する領域を対象エリアとして自由に定めればよい。選定後は、面積、外周距離、空間条件（閉鎖空間か開放空間か）、栽培作物種等の対象エリアに関する様々な情報を獲得しておく。
(1) Target area selection step The "target area selection step" is a step of specifically selecting a target area in which the stag beetle is to be killed. This is because if the conditions and conditions of the target area cannot be grasped, efficient killing cannot be performed. Therefore, this step is a reference step in the subsequent steps of the present method. The target area is not particularly limited. The region desired by the practitioner may be freely determined as the target area. After the selection, various information on the target area such as the area, the outer peripheral distance, the space condition (closed space or open space), cultivated crop species, etc. is acquired.
（２）捕虫装置設置工程
「捕虫装置設置工程」は、対象エリア内に第３態様に記載の捕虫装置を設置する工程である。本工程では、前記対象エリア選定工程で得られた対象エリアに関する情報に基づいて、対象エリア内に生息するクロテンコナカイガラムシのオス成虫を効率的に誘引し、捕殺できるように捕虫装置を設置する。設置条件については、以下の通りである。
(2) Insect trap installation step The “insect trap installation step” is a step of installing the insect trap according to the third aspect in the target area. In this step, based on the information on the target area obtained in the target area selection step, an insect trapping device is installed so as to efficiently attract and kill male adults of the scorpion scale insects living in the target area. The installation conditions are as follows.
捕虫装置の設置場所は、限定はしないが、クロテン用性誘引物質が対象エリア全体に拡散されやすい場所が好ましい。例えば、対象エリア内の風上等は好ましい。 The installation place of the insect trapping device is not limited, but is preferably a place where the sable attractant is easily diffused throughout the target area. For example, windward in the target area is preferable.
捕虫装置の設置密度は、限定はしないが、屋外であれば0.1〜100個/100m2、好ましくは1〜50個/100m2又は2〜25個/100m2であればよい。また、屋内であれば0.1〜50個/100m2、好ましくは1〜20個/100m2であればよい。 The application density of the insect capturing apparatus, without limitation, as long as outdoor 0.1 pieces / 100 m 2, preferably may be a 1-50 / 100 m 2 or from 2 to 25 pieces / 100 m 2. In the case of indoors, it may be 0.1 to 50 pieces / 100 m 2 , preferably 1 to 20 pieces / 100 m 2 .
本発明の各捕虫装置の設置間隔は、限定はしないが、通常は1m〜100mが好ましく、2m〜50m、3m〜20m、又は5〜15mがより好ましい。 The installation interval of each insect trapping device of the present invention is not limited, but is usually preferably 1 m to 100 m, more preferably 2 m to 50 m, 3 m to 20 m, or 5 to 15 m.
捕虫装置の設置位置（設置高）は、限定はしないが、クロテン用性誘引物質の拡散効率を高めるため地上10cm〜200cm、好ましくは地上20cm〜180cm又は地上30cm〜160cmの高さがよい。 The installation position (installation height) of the insect trapping device is not limited, but is preferably 10 cm to 200 cm above the ground, preferably 20 cm to 180 cm above the ground, or 30 cm to 160 cm above the ground in order to increase the diffusion efficiency of the sable attractant.
捕虫装置の設置時期は、限定はしないが、本種オス成虫発生期間、例えば、露地では4月〜11月が好ましく、5月〜10月がより好ましい。 The installation time of the insect trapping device is not limited, but the adult period of the male species of this species is, for example, preferably from April to November and more preferably from May to October in the open field.
（３）性誘引組成物交換工程
「性誘引組成物交換工程」は、対象エリアに設置した捕虫装置内における性誘引組成物を交換する工程である。
(3) Sex-attracting composition exchange step The “sex-attracting composition exchange step” is a step of exchanging the sex-attracting composition in the insect trap installed in the target area.
本方法は、捕虫装置に備えられた性誘引組成物が包含する有効成分、クロテン用性誘引物質の誘因作用によって、クロテンコナカイガラムシのオス成虫を装置内に誘引する原理に基づく。したがって、性誘引組成物中のクロテン用性誘引物質が放出により枯渇すると誘引効果が失われ、目的を達成し得ない。そこで、クロテン用性誘引物質が枯渇する前に、定期的に性誘引組成物を補充又は交換する必要がある。本工程では、その補充又は交換を目的としている。 The present method is based on the principle of attracting a male adult scorpion scale to the inside of the insect trapping apparatus by the attracting action of the active ingredient contained in the sex attracting composition provided in the insect trapping apparatus, the sex attractant for sable. Therefore, if the sex attractant for sable in the sex attractant composition is depleted by release, the attracting effect is lost and the object cannot be achieved. Therefore, it is necessary to periodically replenish or replace the sex attractant composition before the sex attractant for sable is depleted. This step aims at replenishment or replacement.
性誘引組成物の補充は、捕虫装置内の性誘引組成物が予め設定された所定量を下回った時に、随時追加するように行えばよい。新たに補充する性誘引組成物の剤形やクロテン用性誘引物質の含有量等については、捕虫装置内の性誘引組成物のそれと同じでよいが、必ずしも同一にする必要はない。性誘引組成物が担体に担持又は内蔵されている場合には、担体と共に追加補充することができる。 Replenishment of the sex attraction composition may be performed at any time when the sex attraction composition in the insect trap falls below a predetermined amount. The dosage form of the sex-attracting composition to be newly replenished, the content of the sex-attracting substance for sable, and the like may be the same as those of the sex-attracting composition in the insect trap, but they need not necessarily be the same. When the sex attraction composition is carried or incorporated in a carrier, it can be additionally supplemented together with the carrier.
性誘引組成物の交換は、捕虫装置内の性誘引組成物に含まれるクロテン用性誘引物質が枯渇する前に性誘引組成物ごと新旧入れ替えればよい。性誘引組成物が担体に担持又は内蔵されている場合には、担体ごと交換することができる。交換期間は、対象エリアの面積、空間条件、徐放性担体の使用、捕虫器の形態によって異なるため、対象エリアの条件に持ちづいて適宜定めればよい。例えば、面積100m2（1アール）の屋外対象エリアに設置された捕虫装置において、徐放性担体に担持された性誘引組成物を交換する場合であれば、徐放性担体ごと10日〜14日おきに、粘着トラップに担持された性誘引組成物を粘着トラップごと交換する場合であれば、5日〜7日おきに交換することが好ましい。 The sex attraction composition may be replaced by replacing the sex attraction composition with the old and new ones before the sable sex attraction substance contained in the sex attraction composition in the insect trap apparatus is depleted. When the sex attraction composition is carried or incorporated in a carrier, the carrier can be replaced together. The replacement period varies depending on the area of the target area, the space condition, the use of the sustained-release carrier, and the form of the insect trap, and may be appropriately determined according to the conditions of the target area. For example, in an insect trapping device installed in an outdoor target area having an area of 100 m 2 (1 are), when exchanging a sex-inducing composition carried on a sustained-release carrier, if the sustained-release carrier is exchanged for 10 days to 14 days. In the case where the sex attraction composition carried on the adhesive trap is replaced every other day together with the adhesive trap, it is preferable to replace the composition every 5 to 7 days.
Ｂ．交信撹乱方法
交信撹乱方法は、対象エリア内におけるクロテンコナカイガラムシの繁殖手段、具体的には交尾機会を剥奪して、対象エリアから本種を徐々に排除していく方法である。本方法は、対象エリアの選定工程、交信撹乱装置の設置工程、装置内の性誘引組成物の交換工程を含む。対象エリアの選定工程、及び性誘引組成物の交換工程の基本構成は、それぞれ、上記捕殺方法における対象エリア選定工程、及び性誘引組成物交換工程に準ずる。したがって、ここでは、本方法に特有の交信撹乱装置設置工程、及び性誘引組成物交換工程における固有の構成についてのみ説明をする。
B. Communication Disturbing Method The communication disturbing method is a method of gradually removing the species from the target area by depriving the breeding means of the stag beetle, specifically the mating opportunity, in the target area. The method includes a step of selecting a target area, a step of installing a communication disruption device, and a step of exchanging a sex-inducing composition in the device. The basic configuration of the target area selection step and the sex attraction composition replacement step is based on the target area selection step and the sex attraction composition replacement step in the above-described trapping method, respectively. Therefore, here, only the unique configuration in the communication disrupting device setting step and the sex attraction composition exchanging step which are specific to the present method will be described.
（１）交信撹乱装置設置工程
「交信撹乱装置設置工程」は、対象エリア内に第４態様に記載の交信撹乱装置を設置する工程である。本工程では、前記対象エリア選定工程で得られた対象エリアに関する情報に基づいて、対象エリアにクロテン用性誘引物質を放出及び拡散させて、対象エリア内に生息する本種オス成虫のメス成虫探索行動を撹乱させるように交信撹乱装置を設置する。設置条件については、以下の通りである。
(1) Communication Disturbing Device Setting Step The “communication disturbing device setting step” is a step of setting the communication disturbing device according to the fourth aspect in the target area. In this step, based on the information on the target area obtained in the target area selection step, the sex attractant for sable is released and diffused in the target area, and the female adult of the male adult of this species living in the target area is searched. A communication disrupter will be installed to disrupt behavior. The installation conditions are as follows.
交信撹乱装置の設置密度は、限定はしないが、屋外であれば5〜50個/100m2、又は10〜30個/100m2であればよい。また、屋内であれば1〜30個/100m2、又は5〜10個/100m2でよい。 The application density of the mating disruption device include, but are not limited to, as long as outdoor 5 to 50/100 m 2, or may be a 10-30 / 100 m 2. In the case of indoors, the number may be 1 to 30 pieces / 100 m 2 , or 5 to 10 pieces / 100 m 2 .
交信撹乱装置の設置間隔は、限定はしないが、0.5m〜100m又は1m〜50m、好ましくは1.4m〜10m又は2〜5mである。
交信撹乱装置の設置位置（設置高）及び設置時期は、上記捕虫装置と同じでよい。
The installation interval of the communication disrupting device is not limited, but is 0.5 m to 100 m or 1 m to 50 m, preferably 1.4 m to 10 m or 2 to 5 m.
The installation position (installation height) and installation time of the communication disrupting device may be the same as those of the above insect trapping device.
（２）性誘引組成物交換工程
本工程の基本構成は、捕殺方法に記載の性誘引組成物交換工程に準じる。
本方法の場合、交信撹乱装置に備えられた性誘引組成物が包含する有効成分、クロテン用性誘引物質の放出及び拡散によって、対象エリア内のクロテンコナカイガラムシのオス成虫におけるメス成虫探索行動を拡散させて、交尾機会を剥奪する原理に基づく。したがって、捕殺方法と同様に、性誘引組成物中のクロテン用性誘引物質が放出により枯渇すると交信撹乱効果が失われ、目的を達成し得ない。そこで、クロテン用性誘引物質が枯渇する前に、定期的に性誘引組成物を補充又は交換する必要がある。本工程では、その補充又は交換を目的としている。
(2) Sexually attracting composition exchange step The basic configuration of this step conforms to the sex attractant composition exchange step described in the killing method.
In the case of the present method, the active ingredient contained in the sex attractant composition provided in the communication disrupting device, the release and diffusion of the sex attractant for sable are used to diffuse the female adult search behavior in the male adult scorpion scale in the target area. It is based on the principle of depriving mating opportunities. Therefore, similarly to the killing method, when the sex attractant for sable in the sex attractant composition is depleted by release, the communication disrupting effect is lost and the object cannot be achieved. Therefore, it is necessary to periodically replenish or replace the sex attractant composition before the sex attractant for sable is depleted. This step aims at replenishment or replacement.
性誘引組成物の補充は、基本的に捕殺方法に記載のそれに準ずる。交換期間は、例えば、面積100m2（1アール）の屋外対象エリアに設置された捕虫装置において、徐放性担体に担持された性誘引組成物を交換する場合であれば、徐放性担体ごと30日〜60日おきに交換することが好ましい。具体例として、対象エリアの圃場において、適当な間隔で網又はロープを張り、その網又はロープに性誘引組成物を噴霧又は塗布により担持させる方法が挙げられる。性誘引組成物の担持量は、本発明の性誘引物質の量として、0.1g/100m2〜50g/100m2、好ましくは0.25g/100m2〜25g/100m2を使用すればよい。 Replenishment of the sex attraction composition is basically the same as that described in the killing method. The exchange period is, for example, in the case of exchanging the sex-inducing composition carried on the sustained-release carrier in an insect trap installed in an outdoor target area having an area of 100 m 2 (1 are), It is preferable to replace every 30 to 60 days. As a specific example, there is a method in which a net or rope is stretched at an appropriate interval in a field in a target area, and the net or rope is loaded with the sex attraction composition by spraying or coating. Loading amount of sex attractant composition, as the amount of sex attractant of the present invention, 0.1g / 100m 2 ~50g / 100m 2, may be preferably used to 0.25g / 100m 2 ~25g / 100m 2 .
５−３−２．発生調査方法
発生調査方法では、第３態様に記載の捕虫装置を用いて、対象エリア内のクロテンコナカイガラムシを防除するため、分布状況及び発生状況を管理する。この方法によって、対象エリア内に生息する本種オス成虫の分布状況及び発生状況を把握し、前記駆除方法における具体的かつ効率的な対応策に反映させることができる。本方法は、対象エリアの選定工程、捕虫装置の設置工程、捕虫器回収工程、捕虫個体統計分析工程、捕虫器再設置工程、及び装置内の性誘引組成物の交換工程を必須の工程として含む。このうち対象エリア選定工程、捕虫装置の設置工程、及び装置内の性誘引組成物の交換工程は、上記捕殺方法に記載の各工程と同じであることから、その説明を省略し、ここでは本方法に特有の捕虫器回収工程、及び統計分析工程、及び捕虫器再設置工程について、以下で説明をする。
5-3-2. In the outbreak investigation method, the distribution state and the outbreak state are managed in order to control the stag beetle in the target area using the insect trap according to the third aspect. With this method, it is possible to grasp the distribution status and occurrence status of this species of male adult living in the target area, and to reflect the situation in a specific and efficient countermeasure in the above-described extermination method. The method includes, as essential steps, a step of selecting a target area, a step of installing a trapping apparatus, a step of collecting traps, a step of analyzing individual traps, a step of resetting the traps, and a step of replacing the sex attractant composition in the trap. . Among them, the target area selection step, the installation step of the insect trapping apparatus, and the step of replacing the sex attraction composition in the apparatus are the same as the steps described in the above-mentioned killing method, and therefore the description thereof is omitted, and the present description is omitted here. The insect trap collecting step, the statistical analysis step, and the insect trap re-installation step that are unique to the method will be described below.
（１）捕虫器回収工程
「捕虫器回収工程」は、対象エリアに設置後、所定の期間を経過した捕虫装置から捕虫器を回収する工程である。捕虫器の捕獲手段と保持手段が別個の場合には、保持手段のみを回収すればよい。また、性誘引組成物含有粘着トラップのように捕虫器が性誘引組成物、担体と一体化している場合には、一括で回収してもよい。
(1) Insect trap collection step The “insect trap collection step” is a step of collecting traps from a trap device that has passed a predetermined period after being installed in the target area. When the trapping means and the holding means of the insect trap are separate, only the holding means may be collected. When the insect trap is integrated with the sex attractant composition and the carrier as in the case of the sex attractant composition-containing adhesive trap, the insect traps may be collectively collected.
（２）統計分析工程
「捕虫個体統計分析工程」は、捕虫装置から回収した捕虫器において、捕獲された本種オス個体数を計数し、その捕虫装置の設置情報（設置場所、設置密度、設置間隔、設置位置及び設置期間）と関連付けてデータ化する工程である。単独データで、又は過去のデータと共に、統計学的に処理することで、対象エリア内におけるクロテンコナカイガラムシの一時的又は経時的な分布状況、及び／又は発生状況を分析することができる。ここで得られた分析結果に基づいて、対象エリアにおける効率的な捕殺方法又は交信撹乱方法を策定することができる。
捕獲された本種オス個体数の計数方法は、特に限定はしない。通常は目視による計数でよい。
(2) Statistical analysis step The “statistical analysis step for individual insects” counts the number of male individuals of this species captured in the insect trap collected from the insect trap, and sets the installation information (installation location, installation density, (Interval, installation position and installation period). Statistical processing with single data or together with past data enables analysis of the temporal or temporal distribution and / or occurrence of the stag beetle in the target area. Based on the analysis results obtained here, it is possible to formulate an efficient killing method or communication disruption method in the target area.
The method of counting the number of captured male individuals of this species is not particularly limited. Normally, counting by visual inspection is sufficient.
（３）捕虫器再設置工程
「捕虫器再設置工程」は、対象エリアに設置された捕虫装置を継続して使用するために、上記捕虫器回収工程で回収した捕虫器に換わる新たな捕虫器を捕虫装置に再び設置する工程である。再設置される捕虫器は、以前の捕虫器と同じ構成でよいが、捕虫装置に設置可能な限り他の構成の捕虫器に変更してもよい。捕虫器の捕獲手段と保持手段が別個の場合には、回収した保持手段のみを再設置すればよい。また、捕虫器が性誘引組成物や担体と一体化している場合には、それらと共に交換することで再設置することも可能である。
(3) Insect trap re-installation step The “insect trap re-installation step” is a new trap that replaces the trap collected in the trap trap recovery step in order to continue using the trap trap installed in the target area. Is installed in the insect trap again. The relocated insect trap may have the same configuration as the previous trap trap, but may be changed to another trap trap as long as it can be installed in the trap device. When the catching means and the holding means of the insect trap are separate, only the collected holding means may be reinstalled. In addition, when the insect trap is integrated with the sex attractant composition or carrier, it can be replaced by replacing it with them.
以下に、本発明の内容を実施例によりさらに具体的に示すが、本発明は本実施例の記載に限定されるものではない。 Hereinafter, the content of the present invention will be described more specifically with reference to examples, but the present invention is not limited to the description of the examples.
＜実施例１：クロテンコナカイガラムシの性誘引物質の単離と同定＞
（目的）
クロテンコナカイガラムシのメス成虫から性誘引物質を単離し、その化学物質を同定する。
<Example 1: Isolation and identification of sex attractant of stag beetle>
(Purpose)
Isolate a sex attractant from the adult female stag beetle and identify its chemicals.
（方法）
0.2リットルのガラス容器に羽化後7日から30日前後の未交尾状態のクロテンコナカイガラムシメス成虫を500頭程、ソラマメに寄生させて密閉し、容器内に放出された性誘引物質を含む揮発性化合物をエアポンプにより吸引した。その後、1.5gの吸着剤（テナックスTA、メッシュサイズ20-35；GLサイエンス社製）で捕集した。この操作を繰り返して、約10000頭のメスが一日当たりに放出する量の性誘引物質を回収し、約500mLのヘキサンに溶出したものを粗抽出液とした。1平方cmの濾紙に0.1メス当量（0.1頭のメスが一日当たりに放出する量に相当する性誘引物質量）の粗抽出液を含浸させた後、10頭のクロテンコナカイガラムシオス成虫を入れた直径9cmのシャーレにその濾紙を投入したところ、10頭全ての個体（100%）が濾紙に誘引された。この検定結果から、粗抽出液には目的とするクロテンコナカイガラムシの性誘引物質が含まれていることが示唆された。
(Method)
Approximately 500 non-mating scorpion larva female females, which are not mated about 7 to 30 days after emergence in a 0.2-liter glass container, are parasitized in fava bean, hermetically sealed, and contain volatile compounds containing sex attractants released into the container. The compound was aspirated by an air pump. Then, it collected by 1.5 g of adsorbents (Tenax TA, mesh size 20-35; manufactured by GL Science). This operation was repeated to collect an amount of the sex attractant released by about 10,000 females per day, and the substance eluted in about 500 mL of hexane was used as a crude extract. A 1 cm square filter paper was impregnated with a crude extract of 0.1 female equivalent (the amount of sex attractant equivalent to the amount released by 0.1 females per day), and then 10 adult scutellaria larvae were added. When the filter paper was placed in a petri dish having a diameter of 9 cm, all 10 individuals (100%) were attracted to the filter paper. The results of the assay suggested that the crude extract contained the sex attractant of the desired scutellaria scales.
次に、0.2gのシリカゲルを充填したカラムクロマトグラフに、前記粗抽出液を注入し、ジエチルエーテルを0、5、15、及び50%含むペンタンをそれぞれ2mLずつ用いて分画し、溶出した。上記と同じ濾紙を用いた方法でオス成虫の誘引性を検定したところ、0.1メス当量の5%ジエチルエーテル画分に対して30頭中26頭の個体（87%）が誘引された。 Next, the crude extract was injected into a column chromatograph packed with 0.2 g of silica gel, and fractionated and eluted with 2 mL each of pentane containing 0, 5, 15, and 50% of diethyl ether. When the attractiveness of the adult male was tested by the same method using the same filter paper as above, 26 out of 30 individuals (87%) were attracted to the 0.1 female equivalent of 5% diethyl ether fraction.
続いて、この5%ジエチルエーテル画分をInertsil SIL 100Aカラム（粒径5μm、内径4.6mm、長さ250mm；GLサイエンス社製）を備えた高速液体クロマトグラフ装置によって、さらに分画した。ジエチルエーテルを3%含むペンタンを流速1ml/分で流し、7.3分から8.0分に溶出してきた画分を回収した。上記と同様の方法で、0.1メス当量の画分を30頭のオスに提示したところ、28頭（93%）が誘引された。 Subsequently, the 5% diethyl ether fraction was further fractionated by a high-performance liquid chromatograph equipped with an Inertsil SIL 100A column (particle size: 5 μm, inner diameter: 4.6 mm, length: 250 mm; manufactured by GL Sciences). Pentane containing 3% of diethyl ether was flowed at a flow rate of 1 ml / min, and the fraction eluted from 7.3 to 8.0 minutes was collected. When a fraction of 0.1 female equivalent was presented to 30 males in the same manner as above, 28 (93%) were attracted.
さらに、この画分をDB-23カラム（内径0.53mm、長さ15m；J&Wサイエンティフィック社製）を据え付けたガスクロマトグラフ装置（アジレント・テクノロジー社製）によってさらに分画した。カラムオーブン内の温度を、初期温度50℃（1分間保持）から毎分8℃ずつ200℃まで昇温する分析条件で、15.2分から15.7分に溶出される画分を回収し、0.1メス当量を30頭のオスに提示したところ、30頭（100%）が誘引された。この画分は、単一の物質のみを含み、その物質がクロテンコナカイガラムシのオス成虫に対して誘引活性を有する性誘引物質（性フェロモン）であることが確認された。 This fraction was further fractionated by a gas chromatograph (Agilent Technology) equipped with a DB-23 column (inner diameter 0.53 mm, length 15 m; manufactured by J & W Scientific). Under the analytical conditions of raising the temperature in the column oven from the initial temperature of 50 ° C (holding for 1 minute) to 200 ° C at 8 ° C per minute, collect the fraction eluted from 15.2 minutes to 15.7 minutes, When presented to 30 males, 30 (100%) were attracted. This fraction contained only a single substance, and it was confirmed that the substance was a sex attractant (sex pheromone) having attracting activity to male adults of the scutellaria scaleworm.
この物質を質量分析装置（日本電子社製）により分析した。高分解能質量分析で分子量は236.17845と測定され、分子式はC15H24O2と推定された。EIマススペクトル［m/z （相対強度%）］は次の通りである；55 (9.1), 83 (31.9), 93 (14.8), 105 (8.7), 121 (100), 136 (37.7), 236 (0.3) 。 This substance was analyzed using a mass spectrometer (manufactured by JEOL Ltd.). High-resolution mass spectrometry determined the molecular weight to be 236.17845, and the molecular formula was estimated to be C 15 H 24 O 2 . The EI mass spectra [m / z (% relative intensity)] are as follows; 55 (9.1), 83 (31.9), 93 (14.8), 105 (8.7), 121 (100), 136 (37.7), 236 (0.3).
この物質をさらに核磁気共鳴装置（NMR；日本電子社製）により分析した。1H-NMRの測定値は次の通りである；δH: 5.75 (1H, s), 4.23 (2H, d, J = 8.4), 2.51 (1H, m), 2.23 (1H, m), 2.13 (3H, s), 2.12 (1H, s), 1.51 (3H, s), 1.43 (3H, s), 1.40 (3H, s), 1.25 (3H, s), 1.14 (3H, s)。 This substance was further analyzed by a nuclear magnetic resonance apparatus (NMR; manufactured by JEOL Ltd.). The measured values of 1 H-NMR are as follows; δ H : 5.75 (1H, s), 4.23 (2H, d, J = 8.4), 2.51 (1H, m), 2.23 (1H, m), 2.13 (3H, s), 2.12 (1H, s), 1.51 (3H, s), 1.43 (3H, s), 1.40 (3H, s), 1.25 (3H, s), 1.14 (3H, s).
（結果）
異常の分析結果から、単離された物質は、前述の式１で示される化学構造を有する新規エステル化合物「セネシオ酸マコネリル」であることが推定された。
(result)
From the result of the analysis of the abnormality, it was estimated that the isolated substance was a novel ester compound “maconeryl sensioate” having the chemical structure represented by the above-mentioned formula 1.
＜実施例２：クロテンコナカイガラムシの性誘引物質の製造＞
（目的）
実施例１で単離・同定されたクロテン用性誘引物質を化学合成する。
<Example 2: Production of sex attractant of scorpion scale)
(Purpose)
The sex attractant for sable isolated and identified in Example 1 is chemically synthesized.
（方法）
ZHANG, A. and NIE, J.（2005. J. Agric. Food Chem. 53:2451-2455）に記載の公知の方法で合成した15.4mgのマコネリオールと15.0mgのセネシオ酸（東京化成工業社製）を0.35mLのジクロロメタン中で混合した。18.9mgのジイソプロピルカルボジイミド（和光純薬社製）及び18.3mgの4-ジメチルアミノピリジン（東京化成工業社製）を加え、40℃で1昼夜エステル化して（2,2-dimethyl-3-(propan-2-ylidene)cyclobutyl)methyl 3-methylbut-2-enoate（セネシオ酸マコネリル）を合成した。
(Method)
ZHANG, A. and NIE, J. (2005. J. Agric. Food Chem. 53: 2451-2455) synthesized 15.4 mg of maconeriol and 15.0 mg of senesioic acid (manufactured by Tokyo Chemical Industry Co., Ltd.). ) Was mixed in 0.35 mL of dichloromethane. 18.9 mg of diisopropylcarbodiimide (manufactured by Wako Pure Chemical Industries, Ltd.) and 18.3 mg of 4-dimethylaminopyridine (manufactured by Tokyo Chemical Industry Co., Ltd.) were added, and the mixture was esterified at 40 ° C. for one day and night (2,2-dimethyl-3- (propan). -2-ylidene) cyclobutyl) methyl 3-methylbut-2-enoate (Maconeryl Senecioate) was synthesized.
また、実施例１に記載の方法に準じて、合成したエステル化合物0.1mgを濾紙に含浸させて、オス成虫の誘引性を検定した。 Further, according to the method described in Example 1, 0.1 mg of the synthesized ester compound was impregnated into filter paper, and the attractiveness of male adults was tested.
（結果）
合成したエステル化合物の収率は98%であった。エステル化合物をガスクロマトグラフ・質量分析計（GC-MS）によって分析した。GC-MSはAgilent 6890/5973（アジレント・テクノロジー社製）にDB-1カラム（内径0.25mm、長さ30m；J&Wサイエンティフィック社製）を据え付け、カラムオーブン内の温度を、初期温度60℃（1分間保持）から毎分8℃ずつ220℃まで昇温する分析条件に設定したものを用いた。本実施例で得られたエステル化合物は、クロテンコナカイガラムシのメス成虫から捕集し、単離した性誘引物質と保持時間（13.86分）及びEIマススペクトル［m/z （相対強度%）；55 (9.1), 83 (31.9), 93 (14.8), 105 (8.7), 121 (100), 136 (37.7), 236 (0.3)］が完全に一致した。ガスクロマトグラフ・質量分析計は、Agilent 6890/5973（アジレント・テクノロジー社製）にDB-1カラム（内径0.25mm、長さ30m；J&Wサイエンティフィック社製）を据え付け、カラムオーブン内の温度を、初期温度60℃（1分間保持）から毎分8℃ずつ220℃まで昇温する分析条件に設定したものを用いた。
また、オス成虫の誘引性検定では、多数のオス成虫が誘引された。
(result)
The yield of the synthesized ester compound was 98%. The ester compound was analyzed by a gas chromatograph / mass spectrometer (GC-MS). For GC-MS, a DB-1 column (0.25 mm ID, 30 m length; J & W Scientific) was installed on an Agilent 6890/5973 (Agilent Technology), and the temperature in the column oven was raised to an initial temperature of 60 ° C. (Hold for 1 minute) and set the analysis conditions to raise the temperature to 220 ° C. at 8 ° C./min. The ester compound obtained in this example was collected from a female adult of the scutellid scale insect, the isolated sex attractant, the retention time (13.86 minutes) and the EI mass spectrum [m / z (relative intensity%); 55 (9.1), 83 (31.9), 93 (14.8), 105 (8.7), 121 (100), 136 (37.7), 236 (0.3)]. The gas chromatograph / mass spectrometer is equipped with an Agilent 6890/5973 (manufactured by Agilent Technologies) with a DB-1 column (0.25 mm ID, 30 m long; manufactured by J & W Scientific), and the temperature in the column oven is measured. The analysis conditions were set such that the temperature was raised from an initial temperature of 60 ° C. (held for 1 minute) to 220 ° C. at 8 ° C. per minute.
In the test for attracting male adults, many male adults were attracted.
以上の結果から、クロテン用性誘引物質はセネシオ酸マコネリルであることが確認された。 From the above results, it was confirmed that the sex attractant for sable was maconeryl sensioate.
＜実施例３：クロテンコナカイガラムシ用性誘引物質の種特異性の検証＞
（目的）
実施例２で化学合成したセネシオ酸マコネリルがクロテンコナカイガラムシのオス成虫を種特異的に誘引できることを確認する。
<Example 3: Verification of the species specificity of the sex attractant for black stag beetle>
(Purpose)
It is confirmed that maconeryl senecionate chemically synthesized in Example 2 can specifically attract male adult scutellaria scales.
（方法）
0.1mgのセネシオ酸マコネリルを0.1mLのヘキサンに溶解し、これを検証用とした。陰性対照用（比較用）には0.1mLのヘキサンのみからなる溶液とした。それぞれのヘキサン溶液をゴムキャップに塗布して、クロテンコナカイガラムシのオス成虫と、同じコナカイガラムシ科に属するフタスジコナカイガラムシ（Ferrisia virgata）のオス成虫ぞれぞれ10頭に提示し、誘引されるオス成虫の数を調べた。
(Method)
0.1 mg of maconeryl sensioate was dissolved in 0.1 mL of hexane and used for verification. A solution containing only 0.1 mL of hexane was used as a negative control (for comparison). Each hexane solution is applied to a rubber cap and presented to 10 male adults of the stag beetle and 10 male adults of Ferrisia virgata belonging to the same family of the mealybugs. The number of male adults was determined.
（結果）
表１に結果を示す。
(result)
Table 1 shows the results.
クロテン用性誘引物質であるセネシオ酸マコネリルには、10頭全てのクロテンコナカイガラムシのオス成虫が誘引された（100％）。対して、フタスジコナカイガラムシのオス成虫は、全く誘引されなかった（0％）。 Maconeryl senecioate, a sable attractant, attracted all 10 adult male stag beetles (100%). On the other hand, the adult adults of the giant beetle were not attracted at all (0%).
以上の結果は、セネシオ酸マコネリルがクロテンコナカイガラムシのオス成虫のみを誘引する種特異的性誘引物質であることを示唆している。 The above results suggest that maconeryl sensioate is a species-specific sex attractant that attracts only male adults of the stag beetle, the scale insect.
本発明によれば、クロテンコナカイガラムシのオス成虫を誘引、及び／又は捕獲し、発生状況を知るのに有効な性誘引剤を得ることができる。また、雌雄の交尾行動を撹乱する交信撹乱剤や、オス成虫を誘引除去する大量誘殺剤としても利用することができる。 ADVANTAGE OF THE INVENTION According to this invention, the sex attractant which is effective in attracting and / or capturing the adult male of the scorpion scale can be obtained. Further, it can also be used as a communication disrupting agent that disrupts the mating behavior of males and females, and as a large-scale attractant that attracts and removes adult males.
Claims (9)
請求項１の式（I）で示される化合物からなるクロテンコナカイガラムシの性誘引物質を製造する方法。 Compound represented by the following formula (II)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2016087422A JP6659440B2 (en) | 2016-04-25 | 2016-04-25 | Sex attractant of the scorpion scale |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2016087422A JP6659440B2 (en) | 2016-04-25 | 2016-04-25 | Sex attractant of the scorpion scale |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017197446A JP2017197446A (en) | 2017-11-02 |
| JP6659440B2 true JP6659440B2 (en) | 2020-03-04 |
Family
ID=60238896
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016087422A Active JP6659440B2 (en) | 2016-04-25 | 2016-04-25 | Sex attractant of the scorpion scale |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6659440B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6659439B2 (en) * | 2016-04-25 | 2020-03-04 | 国立研究開発法人農業・食品産業技術総合研究機構 | Sex attractant of the foot insect scale |
| CN116473057B (en) * | 2023-06-15 | 2023-09-08 | 广东省林业科学研究院 | Application of ethyl oleate in attracting solenopsis invicta |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2516076B1 (en) * | 1981-11-10 | 1986-05-16 | Roussel Uclaf | NOVEL CYCLOPROPANE DERIVATIVES, THEIR PREPARATION PROCESS AND THEIR APPLICATION TO THE PREPARATION OF PERFUMING COMPOSITIONS |
| JP4017107B2 (en) * | 2002-11-12 | 2007-12-05 | 独立行政法人農業・食品産業技術総合研究機構 | Novel ester compounds and uses thereof |
| US8062651B1 (en) * | 2004-03-16 | 2011-11-22 | The United States Of America As Represented By The Secretary Of Agriculture | Attractant pheromone for the male pink hibiscus mealybug, Maconellicoccus hirsutus (Green) (Homoptera: Pseudococcidae) |
| JP4734553B2 (en) * | 2006-04-19 | 2011-07-27 | 独立行政法人農業環境技術研究所 | Sex attractant of Fujikonaga scale insect |
| JP5857369B2 (en) * | 2011-06-07 | 2016-02-10 | 国立研究開発法人農業環境技術研究所 | Sex attractant of pine moth scale insect and sex attractant |
| JP6659439B2 (en) * | 2016-04-25 | 2020-03-04 | 国立研究開発法人農業・食品産業技術総合研究機構 | Sex attractant of the foot insect scale |
-
2016
- 2016-04-25 JP JP2016087422A patent/JP6659440B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2017197446A (en) | 2017-11-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kirk et al. | Semiochemicals for thrips and their use in pest management | |
| Kline | Semiochemicals, traps/targets and mass trapping technology for mosquito management | |
| CN105744832B (en) | Insect repellants | |
| CN107467021B (en) | Attractant composition for preventing and treating helicoverpa clarkii | |
| Zhu et al. | Repellency of a wax-based catnip-oil formulation against stable flies | |
| JP6467177B2 (en) | Natural enemy insect attractant component derived from floral scent and its use | |
| Lihuang et al. | Antennal and behavioral responses of Mythimna separata (Walker) to three plant volatiles | |
| JP6659440B2 (en) | Sex attractant of the scorpion scale | |
| JP6659439B2 (en) | Sex attractant of the foot insect scale | |
| Biddinger et al. | Survey of native biocontrol agents of the brown marmorated stink bug in Pennsylvania fruit orchards and adjacent habitat | |
| Seenivasagan et al. | Oviposition pheromones in haematophagous insects | |
| Liu et al. | Non-host plant extracts reduce oviposition of Plutella xylostella (Lepidoptera: Plutellidae) and enhance parasitism by its parasitoid Cotesia plutellae (Hymenoptera: Braconidae) | |
| Hardman et al. | Effects of acaricides, pyrethroids and predator distributions on populations of Tetranychus urticae in apple orchards | |
| CN110742072B (en) | Pest attractant and preparation method and application thereof | |
| CN110786326B (en) | Application of 2-ethylhexanoic acid | |
| KR102185439B1 (en) | Sex Pheromone Composition for Attracting or Disrupting Mating Insect of Eurytomidae Family | |
| Evans et al. | Behavioral and electrophysiological response of cabbage seed weevils (Ceutorhynchus assimilis) to conspecific odor | |
| KR20140120160A (en) | Sex pheromone compound to attract and control Stathmopoda auriferella | |
| Keiser et al. | Attractiveness of cue-lure and the degradation product 4-(p-hydroxyphenyl)-2-butanone to male melon flies in the field in Hawaii | |
| Mallikarjun et al. | Role of fruit volatiles and sex pheromone components in mate recognition in fruit piercing moth Eudocima materna Linnaeus (Lepidoptera: Erebidae) | |
| Tawfik et al. | Efficacy of certain acaricides against Tetranychus urticae and their side effects on natural enemies, Phytoseiulus persimilis and Stethorus gilvifrons | |
| Okonkwo et al. | Insecticidal Potentials and Chemical Composition of Ethanol Extracts from the Leaves of Acanthus montanus on Selected Insect Pests | |
| Mandava | Handbook of Natural Pesticides: Pheromono, Part B, Volume IV | |
| CN108432754A (en) | A kind of insects repellant and its preparation and application method containing octadecanol | |
| CN110720456B (en) | Application of 3, 5-dimethoxytoluene |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20190306 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20191219 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20200121 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20200206 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6659440 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |