CN110786326B - Application of 2-ethylhexanoic acid - Google Patents
Application of 2-ethylhexanoic acid Download PDFInfo
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- CN110786326B CN110786326B CN201911074293.7A CN201911074293A CN110786326B CN 110786326 B CN110786326 B CN 110786326B CN 201911074293 A CN201911074293 A CN 201911074293A CN 110786326 B CN110786326 B CN 110786326B
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- ethylhexanoic acid
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- beet armyworm
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an application of 2-ethyl hexanoic acid, which is an application of the 2-ethyl hexanoic acid in preparation of a pest repellent. After the Chinese rose is infected by powdery mildew, some volatile secondary metabolites are synthesized in the Chinese rose plant body, and the active ingredient 2-ethylhexanoic acid is separated from the volatile secondary metabolites. Generally, healthy Chinese rose plants cannot synthesize the compound, and the compound can be synthesized only after the Chinese rose is infected with powdery mildew and is induced by the powdery mildew. Through olfactory behavior biological test and field oviposition test, the 2-ethylhexanoic acid has good repellent effect on pests, particularly female beet armyworm. And 2-ethyl caproic acid is a natural secondary metabolite and has a complete degradation way in the nature, so 2-ethyl caproic acid is used as a main component to prepare the pest repellent, thereby realizing the pollution-free control of pests.
Description
Technical Field
The invention belongs to the technical field of plant protection, and particularly relates to application of 2-ethylhexanoic acid.
Background
Chemical pesticides are one of the main means for controlling plant pests, however, chemical pesticides, especially chemical insecticides, can cause problems of environmental pollution, pest resistance and the like while killing pests. With the increasing requirements of modern agriculture and ecological environment on the performance of chemical pesticides, the research and development risks of new chemical pesticide varieties are increased. The botanical pesticide has the advantages of diverse biological activity (various modes of killing insects, killing bacteria and weeding), no residue, safety to the environment, safety to higher animals and natural enemies, difficult generation of drug resistance of pests and the like, and is a hotspot of research by people at present. In particular, many studies have shown that infestation of host plants by obligate parasitic fungi has an adverse effect on insects, i.e. phytopathogens may adversely affect insects on a co-host by host-plant mediated action in a ternary system of phytopathogens-host plants-insects.
Early researches show that the spodoptera exigua after Chinese China rose is infected with powdery mildew is less in occurrence, which indicates that the spodoptera exigua can identify susceptible hosts and avoid spawning on the susceptible hosts, but the chemical mechanism of identification is still unclear. Beet armywormSpodoptera exigua(Hubner) belongs to the Lepidoptera Noctuidae and is a worldwide intractable pest. Especially, in the greenhouse production process of China rose (namely rose), the beet armyworm is seriously damaged. Further research shows that after the Chinese rose is infected by powdery mildew, volatile secondary metabolites can be synthesized in Chinese rose plants, normally, healthy Chinese rose plants cannot be synthesized with the compounds, and only after the Chinese rose is infected by the powdery mildew, the compounds can be synthesized under the induction of the powdery mildew. Such new compounds induced by powdery mildew may have a negative effect on insects. Meanwhile, in combination with the phenomenon that the spodoptera exigua on Chinese roses is remarkably reduced after the powdery mildew is infected, the volatile compounds are possibly one of important information compounds for identifying host plants infected with the powdery mildew by the spodoptera exigua. The compounds are used as secondary metabolites of plants, have a complete degradation path in the nature, and play an important role in the pollution-free control of pests if researches prove that the compounds have stronger activity on certain pests.
The existing method for preventing and controlling beet noctuids mainly uses sex pheromone to trap male moths to kill the male moths, except chemical prevention and control. But has two problems, one is that the asparagus caterpillar has parthenogenesis phenomenon; secondly, even if only a few male moths are not killed, the mating requirement can be met, and the population density cannot be effectively reduced. Therefore, the control of the female moth is very important. Therefore, a pest repellent which takes an information compound synthesized by inducing host plants by plant pathogenic fungi as a main component and is used for regulating and controlling host searching behaviors and oviposition behaviors of female moths is developed and developed, so that the moths can be more effectively controlled.
Disclosure of Invention
The invention aims to provide application of 2-ethylhexanoic acid.
The purpose of the invention is realized by the application of the 2-ethyl caproic acid in preparing pest repellent.
After the Chinese rose is infected by powdery mildew, some volatile secondary metabolites are synthesized in the Chinese rose plant body, and the active ingredient 2-ethylhexanoic acid is separated from the volatile secondary metabolites. Generally, healthy Chinese rose plants cannot synthesize the compound, and the compound can be synthesized only after the Chinese rose is infected with powdery mildew and is induced by the powdery mildew. Through olfactory behavior biological test and field oviposition test, the 2-ethylhexanoic acid has good repellent effect on pests, particularly female beet armyworm. And 2-ethyl caproic acid is a natural secondary metabolite and has a complete degradation way in the nature, so 2-ethyl caproic acid is used as a main component to prepare the pest repellent, thereby realizing the pollution-free control of pests.
Drawings
FIG. 1 shows the GC-EAD reaction of spodoptera exigua linnaeus female moths on the volatile components of infected Chinese roses;
FIG. 2 is the EAG reaction of spodoptera exigua ovipositor to 2-ethylhexanoic acid;
FIG. 3 is the olfactory behavioral response of spodoptera exigua linza to 2-ethylhexanoic acid;
in the figure, the calculation formula of the repellent rate in the olfactory behavior biological test is
RR(%)＝(N CK－N T)/(N CK + N T)×100%，
Wherein,RRfor the repellency rate,N CKthe number of female moths on the ethanol control side,N Tthe number of female moths on one side of the 2-ethylhexanoic acid,N Nfor the number of unreacted insects, Chi is adopted2The tests were subjected to a statistical analysis which,P <0.05 indicated significant difference;
FIG. 4 is a graph of the effect of 2-ethylhexanoic acid on the oviposition behavior of spodoptera exigua;
in the figure, different lower case letters indicate that the mass concentration of 2-ethylhexanoic acid in the same period is significantly different from that of the Control (CK) (CK)P≤0.05)。
Detailed Description
The present invention is further illustrated by the following examples and the accompanying drawings, but the present invention is not limited thereto in any way, and any modifications or alterations based on the teaching of the present invention are within the scope of the present invention.
The application of the 2-ethyl hexanoic acid is the application of the 2-ethyl hexanoic acid in preparing the pest repellent.
The effective concentration of the 2-ethyl caproic acid in the pest repellent is 0.4-50.0 g/L.
The effective concentration of the 2-ethyl caproic acid in the pest repellent is 10.0 g/L.
The pests are beet armyworms.
The pests are beet armyworm female moths.
The preparation method of the pest repellent comprises the steps of preparation of mother liquor A, preparation of auxiliary liquor B and preparation, and specifically comprises the following steps:
A. preparing a mother solution A: accurately weighing 2-ethylhexanoic acid, adding a solvent to dissolve the 2-ethylhexanoic acid to prepare mother liquor A;
B. preparing an auxiliary liquid B: preparing a tween-80 solution by using deionized water to obtain an auxiliary solution B;
C. preparation: and mixing the mother solution A and the auxiliary solution B, performing ultrasonic treatment, and fixing the volume to obtain the target pest repellent.
The solvent in the step A is one or more of ethanol, normal hexane, methanol and dichloromethane.
And B, the mass percentage concentration of the Tween-80 solution in the step B is 0.1%.
And the ultrasonic treatment in the step C is ultrasonic treatment for 4-6 min at the ultrasonic frequency of 40-60 KHz.
The use method of the pest repellent is that the pest repellent is diluted by 0-125 times with ethanol or water and then directly and uniformly sprayed on buds, branches and leaves of plants.
The working principle of the invention is as follows:
china rose (Rosa chinensisJacq., commonly known as rose) is one of four fresh cut flowers in the world, is deeply loved by people and is widely cultivated all over the world. However, the occurrence of diseases and insect pests in China rose is also very serious, and mainly comprises the Chinese rose powdery mildew [ 2 ]Sphaerotheca pannosa（Wallr. ：Ex Fr.）Lév.]Beet armyworm [ sic ], [ solution of Japanese asparagus ] andSpodoptera exigua（Hübner）]and the like. It has been shown that diseases may have a beneficial or adverse effect on insect pests, and that plant volatile components and their changes play a crucial role in the host selection of insects during pest-pest interactions. A few reports on the influence of powdery mildew on asparagus caterpillar after being infected by China rose, and the powdery mildew is proved to have adverse influence on the asparagus caterpillar. Therefore, volatile components of healthy China roses and China rose infected by powdery mildew are respectively extracted, and the result shows that the volatile components of the healthy China roses have obvious attraction effect on asparagus caterpillar adults; the volatile components of the infected Chinese rose show obvious repellent effect on the beet armyworm imagoes. Further separating and identifying the volatile components of the infected Chinese rose, and finding that the 2-ethyl caproic acid is the main active substance for producing the repellent effect.
The invention has the characteristics that:
the 2-ethyl hexanoic acid is used as an information compound for identifying disease-sensitive hosts of the asparagus caterpillars, is used for replacing chemical pesticides to control the asparagus caterpillars, and cannot generate adverse effects on Chinese rose plants or cause the asparagus caterpillars to generate drug resistance. The invention specially aims at the female moth to prevent and control, and can obviously improve the prevention effect. Meanwhile, the 2-ethyl caproic acid is from host plants, has a natural and complete degradation way in nature, does not generate adverse effect on the environment, is an environment-friendly active component, and has wide application prospect.
Example 1
Discovery of pest repellent substance
(1) Extraction and GC-MS identification of volatile components
Extracting volatile components of healthy Chinese rose leaves and Chinese rose leaves infected with powdery mildew by adopting a headspace dynamic adsorption method, storing the collected volatile components in a refrigerator at the temperature of-20 ℃, and carrying out GC-MS analysis. The adsorbent used in the collection process is Tenax TA, and the collection time is 24 hours, so as to eliminate the influence of the change of the daily rate on the types and the contents of the volatile components.
(2) Method for breeding beet noctuids
Putting the collected beet armyworm mature larva into a 250 mL beaker padded with damp sand, sealing the beaker by using gauze, and transferring the adult insects into another 250 mL beaker after pupation and eclosion. The nutrition is supplemented with 10% honey water, so that the Chinese rose leaves can lay eggs and hatch on healthy Chinese rose leaves, and the Chinese rose leaves are raised to mature larvae (raising conditions: 27 ℃, 70% RH, 12 h photoperiod). Pupating by the same method, taking pupa out under a scope, and breeding female pupa and male pupa separately, wherein the pupa is used for biological test after eclosion.
(3) GC-EAD test of beet armyworm adults
The temperature rise program of the gas chromatograph is consistent with that of the GC-MS; and (4) sampling without shunting, wherein the sampling amount is 2.0 mu L. The recorded signals are connected via an amplifier (Syntech CS-55) to a converter IDAC (Syntech IDAC-2) which is in turn connected to a hardware acquisition card of a computer, and data are acquired and analyzed by computer software (GC-EAD, version 4.6). The tentacles of the beet armyworm adults are cut off from the base part, 2 flagellates at the end part are removed, and then the tentacles are connected with an antennal potentiostat probe by conductive adhesive. The chromatographic column is HP-5 capillary column (30 m × 0.25 mm × 0.25 μm), the outlet is connected with shunt, the split ratio is 1: 1, one part is introduced into FID hydrogen flame ionization detector, and the other part is blown to the antenna through heating jacket (Syntech MP-3), and GC-EAD software is used to synchronously record gas chromatogram and antenna potential diagram. The test insect status is that the beet armyworm has mated female moth. The used antenna is not reused.
(4) Results
Extracting volatile components of healthy and infected powdery mildew Chinese roses by a headspace dynamic adsorption method; whereas the volatile constituents of the powdery mildew itself are extracted by solvent methods to obtain a wider variety of substances, which, if identified from powdery mildew, are not synthesized by the plant itself and will be discarded. And carrying out GC-MS identification to obtain specific chemical components of the Chinese rose living plant volatile matter. As shown in table 1: HRP and IRP are volatile components identified from healthy chinese rose plants (HRP) and chinese rose plants infected with powdery mildew (IRP), respectively. And RPM is extracted from rose powdery mildew (rose powder mildew) hyphae and spores. "-" indicates that no identification was made from this material. GC-MS identifies that the natural compound of the infected Chinese rose plant extract contains 2-ethyl hexanoic acid (Table 1: No. 18). The compound is not derived from powdery mildew itself, but is synthesized by the host plant under the induction of powdery mildew. The compound is not synthesized in healthy China rose plants under normal conditions, so the appearance of the compound is the chemical characteristic of plant infection and is called as an infection marker compound (biomarker of infection). The compound is quite possibly an information compound for identifying a disease-sensitive host by using the spodoptera exigua, and has good activity on the spodoptera exigua, so that the activity of 2-ethylhexanoic acid on the spodoptera exigua is further researched. Meanwhile, the GC-EAD results (fig. 1) indicate that peak No. 1 in fig. 1 produces a clear antennal Electrophysiological (EAG) signal. According to FID information such as retention time of the No. 1 peak in GC-EAD, combining all other peaks, and carrying out comparison analysis with GC-MS, the No. 1 peak is 2-ethylhexanoic acid. The compound can generate an EAG signal, and the compound is one of important information compounds for identifying hosts and searching spawning sites of spodoptera exigua.
TABLE 1 GC-MS analysis of volatile constituents of Health (HRP), infectious disease (IRP) China rose and Chinese Rose Powdery Mildew (RPM)
Table 1 GC-MS analysis for volatiles from healthy rose plants (HRP), infected rose plants (IRP), rose powdery mildew (RPM)
Example 2
EAG reaction of beet armyworm adults to 2-ethylhexanoic acid
And (3) cutting off tentacles of the spodoptera exigua female moths, removing 2 flagellates at the end parts, connecting the cut tentacles with a probe of an tentacle potentiostat by using conductive adhesive, and carrying out an tentacle Electrophysiological (EAG) test. 20.0. mu.L of the sample solution to be tested (2-ethylhexanoic acid-n-hexane solution) was applied uniformly to a filter paper strip (l.times.w = 6.0. times.0.5 cm), placed in a Pasteur tube and connected to a stimulation air stream. The flow rate of the stimulation gas flow was 1150 mL/min and the flow rate of the continuous gas flow was 850 mL/min. After the baseline is stabilized, the foot pedal is used for artificial stimulation, the single stimulation lasts for 0.2s, and the interval is more than 60s each time. The sequence of the sample to be detected in the same antenna is n-hexane (1 time), trans-2-hexenal (3 times), the sample to be detected (3 times) and n-hexane (1 time). Wherein n-hexane is used as a control; the trans-2-hexenal is a standard compound, and the concentration of the sample to be tested and the concentration of the standard compound are both 50.0 g/L. The order of detection of the standard compound and the sample was interchanged after each 1 antenna tested. Each sample was replicated with 4 antennae. The EAG signal was amplified by an amplifier (Syntech CS-55), displayed on an oscilloscope, and normalized by software provided by Syntech corporation for the EAG response value of each sample, and the EAG relative response value = (response value of sample-response value of control)/(response value of standard compound-response value of control) × 100%. A total of four different concentrations (0.4, 2, 10, 50 g/L) of 2-ethylhexanoic acid were tested and the results are shown in FIG. 2.
As can be seen from FIG. 2, 2-ethylhexanoic acid caused a significant EAG reaction. When the spodoptera exigua is used for testing, the relative reaction value of EAG is over 100% except the concentration of 0.4 g/L, which shows that the spodoptera exigua has stronger reaction capability to 2-ethylhexanoic acid than that of the standard compound trans-2-hexenal, and even if the concentration is the lowest (0.4 g/L), the relative reaction value of EAG reaches 92.5% and is basically consistent with that of trans-2-hexenal. However, as the concentration of 2-ethylhexanoic acid increased, the relative reaction value of EAG did not increase proportionally, but the difference was small in comparison between the concentrations of 10.0 and 50.0 g/L.
In the embodiment, the compound is tested by purchasing a standard 2-ethylhexanoic acid, and the compound is confirmed to have activity on the spodoptera exigua and play an important role in the selection process of the spawning place of the spodoptera exigua. It is still unclear whether the compound has an attracting effect or a repelling effect on the beet armyworm adults.
Example 3
Olfactory behavior of beet armyworm females on 2-ethylhexanoic acid
The main instrument used is a Y-type olfactometer. Wherein the main branch of the Y-shaped pipe is 22 cm, the branches at two sides are 17 cm, the inner diameter is 5cm, and the included angle between the two arms is 75 degrees. Cleaning the Y-shaped tube, sterilizing with absolute ethyl alcohol, and drying. A Y-type olfactometer is connected with a silicone tube according to the sequence of an air pump, activated carbon, distilled water, an LZB-II glass rotameter, a three-way pipe and a Y-type pipe, and is horizontally placed, black cloth shades the light, and the air flow is adjusted to be 300 mL/min. The airflow is converged on the main branch of the Y-shaped pipe and flows out from the opening of the base. Clean air was introduced for 10 minutes before the experiment was started.
For slow gas overflow, a small glass bottle with a small hole containing 0.4 g/L2-ethyl hexanoic acid solution is placed into one side branch of a Y-shaped olfactometer, the other side branch is compared with the Y-shaped olfactometer by using ethanol, mated female moths are placed into the small glass bottle from the base opening of the Y-shaped tube, 10 moths are placed at each time, and the small glass bottle is sealed by using black yarns with small holes of 2 mm to prevent adult moths from escaping. The moth can climb or fly to the smell source in a reversible wind manner, identify the smell source and make a response. The number of unreacted insects in the branches at both sides of the Y-shaped tube and in the basal part at 30 min was recorded, and positive reaction was recorded when the insects advanced 3 cm or more from the intersection of the branches at both sides. 2.0, 10.0 and 50.0g/L of the solution are tested by the same method. The test was repeated 5 times, the mean value was taken, and statistical analysis was performed on SPSS 17.0.
After each live test, the direction of the Y-tube and the odor source are exchanged. After the two tests, the Y-shaped tube and the flavor source bottle are taken down, cleaned, disinfected, dried and then subjected to the next bioassay. The used female moth is not reused. The bioassay is carried out at a time interval of 7: 00-12: 00 at night under the conditions of no wind, room temperature of 25 ℃, shading and air humidity of 75%. From the entrance of the base part to the odor source, the 2-ethyl caproic acid forms a concentration gradient from small to large, the beet noctuids mate with the female moths can identify the types and the concentration gradient of the information compounds and make corresponding reactions, namely the principle is consistent with the principle that the beet noctuids identify the hosts infected with powdery mildew in the nature.
The results are shown in FIG. 3. As can be seen from the figure 3, in the olfactory behavior test, 2-ethylhexanoic acid shows an obvious repellent effect on female beet armyworm moths, and the repellent effect is better when the mass concentration is higher. When the mass concentration is 50.0 mg/mL, the maximum repelling rate is 59.5%. Similar to the EAG test results, the differences were not significant when tested at concentrations of 10.0 and 50.0g/L (P= 0.720), the attraction rates were substantially uniform. Compared with the two concentrations of 0.4 and 2.0 g/L, the difference is obvious and reaches a significant level (P < 0.001、P = 0.032）。
Example 4
Repellent effect test of 2-ethylhexanoic acid on oviposition behavior
Sterile soil of about 5cm thickness was placed in a 2-piece 500 mL beaker and moistened with a suitable amount of sterile water. Two bundles of 5 healthy and disease and insect pest free fresh Chinese rose branches with the length of about 20 cm and the number of leaves basically the same are respectively inserted into the soil in 2 beakers, and the two beakers are respectively marked as No. 1 and No. 2. Ethanol is used as a solvent to prepare 0.4 g/L solution of 2-ethyl hexanoic acid, 5 mL of the solution is uniformly sprayed on branches and leaves in a No. 1 beaker, and 5 mL of ethanol is sprayed on branches and leaves in a No. 2 beaker by the same method to serve as a contrast. The two beakers were placed in an egg laying cage made of fine wire mesh at a distance of about 50 cm.
Artificially feeding beet armyworms until pupation, taking the pupae out, distinguishing male and female pupae under a scope, and separately feeding the male and female pupaes until eclosion. 15 pairs of adult beet armyworms which have eclosion, normal development and male-female pairing on the same day are selected and put into a cage for oviposition, and cotton dipped with a proper amount of honey water (10%) is hung on branches to be used as an adult insect nutrient source. Total egg production on both shoots was recorded daily until all female moths no longer lay eggs. After checking the number of eggs laid each time, the fresh branches are replaced and sprayed with 2-ethyl hexanoic acid solution and absolute ethyl alcohol. Room temperature, shading and air humidity of 75 percent. And counting the total egg mass number and total egg yield of each day. Repeat 5 times, SPSS 17.0 for statistical analysis. Three other concentrations (2.0, 10.0, 50.0 g/L) of 2-ethylhexanoic acid were tested in the same manner. The results are shown in FIG. 4.
The results in FIG. 4 show that 2-ethylhexanoic acid had a very significant repellent effect on the selection of the egg producing location of beet armyworm, both in terms of total egg production (FIG. 4A) and total egg mass (FIG. 4B). When the mass concentration is 50.0g/L, the repellent effect is best. Calculated according to the total egg laying amount in fig. 4, the maximum repellency rate is 45.1%. As can be seen from FIG. 4, the test results at the concentrations of 10.0 and 50.0g/L are not very different, and the repelling rates are 40.9% and 45.1% respectively. And at 2.0 g/L, the repellent rate is only 24.9%, and obvious difference exists. Through analysis of test results of an EAG and a Y-type olfactometer, when the 2-ethylhexanoic acid is used as the spodoptera exigua imago repellent, the optimal use concentration is 10.0g/L, and the economic and practical effects can be achieved.
Example 5
Experiment in the field
The field experiment for testing the activity of 2-ethylhexanoic acid on repelling the egg laying behavior of beet armyworm is carried out in a greenhouse of a rose planting base in the tribute area of Kunming City, Yunnan province. No pesticide is used within 14 days before the experiment is started, and normal water and fertilizer management is carried out. The average plant height of China rose is 20 cm at the beginning of the experiment, and the areas of the treatment cell and the control cell are 4 m each2At intervals of 2 m. Wherein, each plant of the treatment district is sprayed with 1 mL of 2-ethylhexanoic acid solution of 10.0g/L to the whole Chinese rose plant, and the control district is sprayed with ethanol with the same volume only by the same method. Putting 15 artificially-fed adult beet armyworm pairs of male and female in a greenhouse, collecting and counting the egg quantity and egg mass number of the adult beet armyworm pairs every day, and counting the total egg production quantity and total egg mass number after 5 days. Let 3 replicates. The same method was used to test a solution containing Tween-80 (2-ethylhexanoic acid-ethanol-Tween 80 solution (2-ethylhexanoic acid-ethanol-Tween 80), diluted with distilled water). The humidity in the greenhouse is 75-95%, the temperature is 18-40 ℃, and other methods and conditions are the same as those of an indoor egg laying amount test experiment. No work such as soil and plant sterilization was required, and the results are shown in Table 2.
TABLE 22 field experiments with ethylhexanoic acid
Table 2 Field experiment for 2-ethylhexanoic acid
As can be seen from the total values of the number of egg masses and the egg laying amount in Table 2, under the actual production conditions of rose planting in a greenhouse, 2-ethylhexanoic acid also shows an obvious repellent effect on egg laying of beet armyworms. From the aspect of egg laying amount, the repellent rate can reach 38.7 percent. The effect of 2-ethylhexanoic acid in repelling female beet armyworm moths was also verified in field experiments. Tween-80 was added to the repellent without significant effect from the viewpoint of insect-repellent effect, but it increased the solubility of 2-ethylhexanoic acid in water, so that ethanol could be replaced with water in the course of practical use.
In summary, the 2-ethylhexanoic acid can effectively play a role in regulating and controlling the selection behavior of the beet armyworm egg-laying place, if the beet armyworm detects the existence of the 2-ethylhexanoic acid, the beet armyworm can be prevented from laying eggs on the plant, the key role is played in the selection process of the beet armyworm egg-laying place, and the information compound can be used for pollution-free control of pests such as the beet armyworm and the like, and has a wide application prospect.
Claims (9)
1. The application of the 2-ethyl hexanoic acid is characterized in that the 2-ethyl hexanoic acid is applied to preparation of a beet armyworm repellent.
2. The use of 2-ethylhexanoic acid as in claim 1, wherein the effective concentration of 2-ethylhexanoic acid in beet armyworm repellent is 0.4-50.0 g/L.
3. The use of 2-ethylhexanoic acid as in claim 1 or 2, wherein the effective concentration of 2-ethylhexanoic acid in the beet armyworm repellent is 10.0 g/L.
4. The use of 2-ethylhexanoic acid as in claim 1, wherein the spodoptera exigua is spodoptera exigua.
5. The application of 2-ethylhexanoic acid as in claim 1, wherein the preparation method of the beet armyworm repellent comprises the steps of preparation of a mother solution A, preparation of an auxiliary solution B and preparation, and specifically comprises the following steps:
A. preparing a mother solution A: accurately weighing 2-ethylhexanoic acid, adding a solvent to dissolve the 2-ethylhexanoic acid to prepare mother liquor A;
B. preparing an auxiliary liquid B: preparing a tween-80 solution by using deionized water to obtain an auxiliary solution B;
C. preparation: and mixing the mother solution A and the auxiliary solution B, performing ultrasonic treatment, and fixing the volume to obtain the target pest repellent.
6. The use of 2-ethylhexanoic acid in the amount of claim 5, wherein the solvent in step A is one or more selected from the group consisting of ethanol, n-hexane, methanol and dichloromethane.
7. The use of 2-ethylhexanoic acid in the amount of 0.1% by weight of the tween-80 solution in step B, according to claim 5.
8. The use of 2-ethylhexanoic acid in the amount of claim 5, wherein the ultrasonic treatment in step C is ultrasonic treatment at an ultrasonic frequency of 40 to 60KHz for 4 to 6 min.
9. The application of 2-ethylhexanoic acid as in claim 1, wherein the beet armyworm repellent is applied by diluting the beet armyworm repellent with ethanol or water 0-125 times, and then directly and uniformly spraying the diluted beet armyworm repellent on flower buds or branches and leaves of plants.
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| 周开艳等.《4种单体化合物对甜菜夜蛾嗅觉行为的影响》.《河南农业科学》.2012,第41卷(第2期),摘要以及第94页左栏第1段. * |
| 李艳等.《感染白粉菌玫瑰对甜菜夜蛾幼虫取食与发育的硬影响》.《西南林学院学报》.2010,第30卷(第2期),第44-46页. * |
| 杨发忠等.《白粉菌侵染中国月季对甜菜夜蛾幼虫乙酰胆碱酯酶活性的影响》.《河南农业科学》.2012,第44卷(第12期),第75-78页. * |
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