CN108378030A - A kind of insects repellant and its preparation and application method containing dodecanol - Google Patents
A kind of insects repellant and its preparation and application method containing dodecanol Download PDFInfo
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- CN108378030A CN108378030A CN201810400895.6A CN201810400895A CN108378030A CN 108378030 A CN108378030 A CN 108378030A CN 201810400895 A CN201810400895 A CN 201810400895A CN 108378030 A CN108378030 A CN 108378030A
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- insects repellant
- dodecanol
- beet armyworm
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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Abstract
The invention discloses a kind of insects repellant containing dodecanol and its preparation and application method, the insects repellant includes dodecanol, can also add the auxiliary elements such as ethyl alcohol equal solvent and neopelex.The repellant has apparent repellent action to pests such as beet armyworms.Also, the repellant main component is Secondary metabolites, can degrade in nature, be a kind of non-harmful insects repellant, have broad application prospects.
Description
Technical field
The invention belongs to technical field of plant protection, further belong to biological pesticide Prevention Technique field, and in particular to one
Insects repellant and its preparation and application of the kind containing dodecanol.
Background technology
As modern agriculture continues to develop, more it is rooted in the hearts of the people to the protection of ecological environment.At the same time, plant protection is managed
It reads and continues to develop variation, known together with " anti-" first to become industry during pest control, rather than harmful organism outburst is
" controlling ", therefore, botanical pesticide comes into being, and the solution for " 3R " problem existing for conventional chemical pesticide provides new way.
Botanical pesticide has diverse biological activities (desinsection, sterilization, weeding and the mode of action are varied), noresidue, pacifies to environment
Entirely, the advantages that being not likely to produce drug resistance to higher mammal and natural enemies security, pest has become the hot spot of people's research at present.This
Wherein, the screening of botanical attractant and repellant causes experts and scholars' highest attention.Attractant and repellant are as information
Compound plays direct key effect during agriculture and forestry injurious insect identifies host, and the concentration of semiochemical is only in air
Need to reach nanogram level or it is lower can effectively be identified by insect, therefore, this kind of semiochemical be used for harmful insect prevention,
Effectively insect lipids can be regulated and controled, have the characteristics that preventive effect is high.In addition, since insect often has learning ability, when
Insect is attracted to destination, finds that no host plant exists, then attractant effect can decline, so the use of attractant exists
Certain risk.And repellant is then different, can effectively avoid the above situation.Certainly, if there is enough attractants and
The type of repellant then can use the two, for the prevention of agriculture and forestry injurious insect simultaneously, to form push-pull system, keep preventive effect aobvious
It writes and improves.Due to the previous more screenings for focusing on attractant of research, more achievement is had been achieved for, large quantities of activity are had accumulated
Preferable compound, therefore, just at present, the screening of repellant seems more urgent.
Have it is more studies have shown that obligatory parasitism fungal infection host plant the insect on same host can be caused it is unfavorable
It influences.Beet armywormSpodoptera exigua(H ü bner) belongs to Lepidoptera, Noctuidae, is a kind of worldwide obstinate pest,
Especially Chinese Chinese rose（That is rose）In greenhouse production process, beet armyworm harm is extremely serious.
For this purpose, develop it is a kind of using plant pathogenic fungi induction host plant synthesis semiochemical be mainly at
The insects repellant divided is used for the prevention of beet armyworm, has a very important significance.
Invention content
An object of the present disclosure is to provide a kind of nuisanceless insects repellant of plant source；Second be designed to provide it is described
The preparation method of insects repellant；Third is designed to provide the application method of the repellant.
What an object of the present disclosure was achieved, the insects repellant includes dodecanol.
What the second purpose of the invention was achieved, the preparation method of the insects repellant includes the following steps：
（1）The preparation of mother liquor A：2.31~6.75g dodecanols accurately are weighed, are dissolved with solvent；
（2）Assist the preparation of liquid B：The neopelex solution that mass fraction is 10% is prepared with deionized water；
（3）10mL B solutions are taken to be mixed with solution A, 4 ~ 6 min mixings of ultrasonic wave；
（4）It is settled to 1 L using solvent, obtains the insects repellant.
What third purpose of the present invention was achieved, the application method of the insects repellant is to use the insects repellant
After ethyl alcohol or water dilute 0 ~ 5 times, Direct Uniform is sprayed on the bud and branches and leaves of plant.
Compared with prior art, beneficial effects of the present invention：
1, the insects repellant containing dodecanol of the invention can make beet armyworm adult pass through the osmoreceptor knowledge of oneself
Do not go out these compounds, will avoid laying eggs on the plant, therefore harm of the worm to plant can be prevented, pest of the invention drives
Prevention of the agent due to being used directly for female moth is kept away, it is therefore more efficient, it avoids because there are single-female generation phenomenons for beet armyworm
And the prevention brought is difficult.
2, the present invention is by Olfactory behavior biological test and field oviposition is tested it is found that dodecanol is to pest, especially
Beet armyworm female adult has good repellent effect.And since dodecanol is natural secondary metabolite, have in nature
Complete degradation pathway；Therefore, insects repellant is made using dodecanol as main component, to realize the nuisanceless of pest
Prevention.
Description of the drawings
Powdery mildew infects the influence to beet armyworm egg laying amount in the experiment of Fig. 1 single choices；
Powdery mildew infects the influence to beet armyworm egg laying amount in the bis- choosing experiments of Fig. 2；
Fig. 3 beet armyworms female moth that do not mate reacts the GC-EAD of susceptible Chinese Chinese rose volatile ingredient；
Fig. 4 beet armyworms female moth that mates reacts the GC-EAD of susceptible Chinese Chinese rose volatile ingredient；
Fig. 5 different quality concentration dodecanols mate to beet armyworm the influence of female moth Olfactory behavior；
In figure, the calculation formula of scattering ratio is in Olfactory behavior biological test
RR(%)=(N CK N T)/(N CK + N T) × 100%,
Wherein,RRFor scattering ratio (repellent rate),N CKFor the female moth number of ethanol control side,N TFor dodecanol side
Female moth number,N NFor unreacted borer population.Using χ2Examine it is for statistical analysis,P<0.05 indicates significant difference；
Influence of Fig. 6 dodecanols to beet armyworm oviposition behavior；
In figure, difference is aobvious compared with control (CK) respectively for each mass concentration of dodecanol in the different lowercase letter same periods
Write (P≤0.05)；Different capitalizations indicate compared with compareing (CK) differences reach the pole level of signifiance (P≤0.01)。
Specific implementation mode
With reference to embodiment and attached drawing, the invention will be further described, but is not limited in any way the present invention
System each falls within the scope of the present invention based on any transformation made by the present invention.
A kind of insects repellant of the present invention includes dodecanol.Dodecanol can be used directly as drive in the present invention
Agent is kept away, solvent and other auxiliary elements can also be added.The solvent is one or more in ethyl alcohol, methanol, n-hexane.
In addition, auxiliary element such as neopelex etc. can be added, neopelex does not interfere with the drive of dodecanol
Worm effect, but the solubility of dodecanol in water can be increased.In actual use, the evil of neopelex is added
Worm repellant can be diluted with water.
Further include surfactant in the insects repellant, the surfactant is in pest repelling agent solution
Solubilising, dispersion are can aid in, while improving pest repelling agent solution in use on the bud and branches and leaves of plant
Spreadability, enhancing effect.
Preferably, the solvent is ethyl alcohol to one kind as the present invention.The matter for the solution that dodecanol is formulated with ethyl alcohol
Measure a concentration of 2.31~6.75 mg/mL.
Preferably, the mass concentration of the solution is 4.18mg/mL to one kind as the present invention.
The preparation method of the insects repellant, includes the following steps：
（1）The preparation of mother liquor A：2.31~6.75g dodecanols accurately are weighed, are dissolved with solvent；
（2）Assist the preparation of liquid B：The neopelex solution that mass fraction is 10% is prepared with deionized water；
（3）10mL B solutions are taken to be mixed with solution A, 4 ~ 6 min mixings of ultrasonic wave；
（4）It is settled to 1 L using solvent, obtains the insects repellant.
The application method of the insects repellant is after the insects repellant ethyl alcohol or water are diluted 0 ~ 5 times, directly
It connects and is uniformly sprayed on the bud and branches and leaves of plant.
The pest is beet armyworm and/or bollworm.
The beet armyworm is the female moth of beet armyworm.
Insects repellant of the present invention can be used for the prevention of the pests such as beet armyworm, bollworm, be driven to beet armyworm
Avoiding effect is best, can especially be used for the most important feature that the female moth of repellent beet armyworm oogenesis is the repellant.
The operation principle of the present invention：
Chinese Chinese rose（Rosa chinensisJacq., it is commonly called as rose）It is one of four big Fresh Cutting flower of the world, it is deep to be liked by people
Love, is widely cultivated in the whole world.But the pest and disease damage generation on Chinese Chinese rose is also extremely serious, includes mainly Sphaerotheca pannosaSphaerotheca pannosa(Wallroth ex Fries) L é veill é, beet armywormSpodoptera exigua
(H ü bner) etc..Some researches show that there may be advantageous or detrimental effects to insect pest for disease, and in disease pest Interaction
In, volatile component of plant and its variation play a crucial role the Tropical area of insect.Powdery mildew infects the Chinese moon
Season has a small amount of report to the research that beet armyworm impacts, it has proved that powdery mildew will produce beet armyworm unfavorable shadow
It rings.The volatile ingredient for the Chinese rose that the healthy Chinese rose of extraction and powdery mildew infect respectively is used to test living to the biology of beet armyworm
Property, the results showed that, healthy Chinese rose volatile ingredient shows apparent attracting action to beet armyworm adult；Susceptible Chinese rose volatility
Ingredient then shows apparent repellent action to beet armyworm adult.Using GC-EAD methods further to susceptible Chinese rose volatility at
Divide and carry out separation identification, it is found that dodecanol therein is the important activity ingredient for generating repellent action.
The features of the present invention：
Semiochemical of the dodecanol as a kind of beet armyworm susceptible host for identification is used for replacing chemical pesticide to sweet tea
Dish noctuid is prevented, and harmful effect will not be generated to Chinese rose plant, the worm will not be caused to develop immunity to drugs.Meanwhile dodecane
Alcohol comes from host plant, there is natural complete degradation pathway in nature, will not generate harmful effect to environment, be one
The environmentally friendly active constituent of kind, has broad application prospects.
Embodiment 1：The discovery of pest repelling substance
(1) beet armyworm method for breeding
The beet armyworm mature larva adopted back is put into the 250 mL beakers for being lined with moist sandy soil, is sealed with gauze, wait pupating,
After emergence, adult is transferred in another 250 mL beaker.It is supplemented the nutrients with 10% hydromel, makes it in Health China Chinese rose
It lays eggs, hatch on blade, and raise to mature larva (rearing conditions：27 DEG C, 70% RH, 12 h photoperiods).It is allowed to change with method
Pupa taking-up is distinguished male and female by pupa under stereoscope, and male and female pupa is separately raised, and biological test is used for after emergence.
(2) inhibiting effect of the host plant infection powdery mildew to beet armyworm Adult worms producting eggs behavior
About 3 cm thickness sterilized soils are padded in 2 500 ml beakers, spill appropriate amounts of sterilized water moist soil.It is new to choose Health China Chinese rose
Each 6 of the Chinese Chinese rose fresh braches (about long 30 cm of branch) of fresh branch and infection powdery mildew, are inserted into 2 beakers respectively
In soil, cannot occur a large amount of liquid waters in beaker, to prevent moth is overboard to be drowned.And the beaker for being plugged branch is respectively put into
In insect cage made of 2 identical wire nettings, black cloth shading.Picked out from the 1 day adult of sprouting wings 8 pairs circle in the air it is normal, female
The beet armyworm adult of hero pairing is put into cage, and the cotton for being moistened with appropriate 10% hydromel, the battalion as adult are hung on branch
The source of supporting.Egg laying amount on later next day observation branch replaces fresh braches after checking oviposition quantity every time.4 days adults after emergence
Start to lay eggs, the 4th, 6,8 day egg laying amount of statistics.It is repeated 4 times.
Health China Chinese rose fresh braches and susceptible Chinese each 6 of Chinese rose fresh braches (about long 30 cm of branch) are chosen, are adopted
It is put into beaker with method identical with single choice biological test and two beakers is placed in simultaneously in same insect cage, diagonally put
It sets, adult, the total egg laying amount of statistics of the pairing of 4 pairs of male and female is accessed with method, is repeated 4 times.The double choosing experiments of egg laying amount use identical side
Method is respectively in three different places（Chenggong, Kunyang and school experiment room, in Chinese Chinese rose planting greenhouse）It carries out, purpose
It is to check whether to affect experimental result because of the particularity of region, expected result has universality, to prevent in reality
It is merely a special case to test the result obtained in room only.
(3) result
Egg laying amount single choice biological test experimental result is as shown in Figure 1.All in all, in single choice biological test, the 4th after emergence
It is all higher than the egg laying amount on susceptible branch to eggs on average amount of the 8th day beet armyworm on Health China Chinese rose branch.It removes
4th day egg laying amount difference is not significantly outer, the 6th day and the 8th day significant difference (P ＜ 0.05).It takes temperature from always, on healthy branch
673.3 ± 41.2；It it is 353.5 ± 33.5 on susceptible branch, difference reaches the pole level of signifiance.
On two kinds of Chinese Chinese rose branches, the egg laying amount of (2 days) is significantly greater in first period, is more than second and third
Egg laying amount in period, extension of the egg laying amount on two kinds of branches all at any time and reduce.On Health China Chinese rose branch, production
The degree of the extension at any time of ovum amount and reduction is not very big.But on susceptible Chinese Chinese rose, the degree of reduction is very big.Feeling
In the single choice biological test carried out on sick China's Chinese rose branch, there is more ovum production on oviposition cage inner wall, and in Health China
On Chinese rose branch, it there is no and observe the upper oviposition on the inside of oviposition cage, but concentrate on laying eggs on blade or on stem.
Double to select biological test the results are shown in Figure 2, the female moth of beet armyworm can freely select in health or sense in experiment
It lays eggs on the Chinese Chinese rose branch of disease.The result shows that beet armyworm is more willing to produce on Health China Chinese rose in double choosings test
Ovum.Total egg laying amount average out to 469.33 of the beet armyworm on Chinese Chinese rose health branch in minute under the conditions of double choosings ±
45.62/worm, the egg laying amount on the Chinese Chinese rose branch after infecting powdery mildew is 166.27 ± 31.52, is produced after susceptible
Ovum amount reduces 64.60%.Per day egg laying amount of the beet armyworm on Health China Chinese rose branch is obviously higher than susceptible China
Per day egg laying amount on Chinese rose branch, and difference is extremely notable（P < 0.01）.It finds simultaneously, beet armyworm is seldom in cage
It lays eggs on wall, it is different when from single choice biological test, show that female moth can be easily in the dark in unsoundness China Chinese rose branch
Identify their host in ground.
The result obtained from Kunyang, the different place in Chenggong and school experiment room three is almost the same, the difference between region
It is different not notable.
In experiment, healthy branch is compared with susceptible branch, and the various non-life conditions including morphology are all consistent
, but beet armyworm can therefrom identify susceptible branch and avoid oviposition, the growth and development wound for its offspring on susceptible branch
Make good condition.During Oviposition site selection, two kinds of unique difference of branch are that volatile ingredient difference, Noctuidae elder brother
Worm beet armyworm can only be identified host by the difference of the two volatile ingredient, thus should the result shows that, Chinese Chinese rose is waved
Hair property ingredient in beet armyworm Adult worms producting eggs plays particularly important effect in selection course, and Chinese rose plant infects powdery mildew
Volatile ingredient afterwards then shows obvious inhibiting effect to beet armyworm adult.
Embodiment 2：The separation and identification of repellent activity ingredient
The volatile ingredient mixture that the Chinese Chinese rose blade of infection powdery mildew is extracted using head space dynamic adsorption method, is used in combination gas phase
Chromatography-electrophysiology combined instrument (GC-EAD) carries out analysis test to the mixture, as a result as shown in Fig. 3, Fig. 4, table 1.It is right
The monomeric compound that can generate apparent EAD signals in mixture is further identified with GC-MS.Wherein, No. 1 peak is dodecane
Alcohol, the content of the compound in the mixture is larger, the EAD signals of generation are stronger.The result shows that being carried from susceptible Chinese rose plant
The native compound (mixture) obtained, wherein containing dodecanol (monomer), it may be that the female moth of beet armyworm is laying eggs
One of the important information compound of susceptible host is identified in ground selection course.
Table 1 causes the female moth of beet armyworm to generate the susceptible Chinese Chinese rose volatile ingredient that EAD reacts
Table 1 The volatiles from healthy rose plants with activity to induce
EAD responses of unmated female moths of beet armyworm
Note：" retention time " refers to the retention time in GC-MS." serial number " is corresponding with the number at peak in Fig. 2, Fig. 3.
Retention time in the table is from GC-MS spectrum. The peak numbers
correspond with the numbers in Figure 2 and 3.
Embodiment 3：Olfactory behavior of the beet armyworm female adult to dodecanol
Key instrument used is Y-shaped smell sensing apparatus.22 cm of Y-piece major branch therein, two side shoots are 17 cm, and internal diameter is 5
Cm, 75 degree of two-arm angle.Y-piece is cleaned, is dried after being sterilized with absolute ethyl alcohol.By Y-shaped smell sensing apparatus by air pump, activated carbon,
Distilled water, LZB-II glass rotameters, three-way pipe, Y-piece sequence are connected with silicone tube, are horizontally arranged, black cloth shading, are adjusted
Solar term flow is 300 mL/min.Air-flow is intersected in Y-piece major branch, is flowed out from base portion mouth.Experiment is first passed through clean sky before starting
Gas 10 minutes.
In order to make gas slowly overflow, by the foraminate vial of band equipped with 2.31 mg/mL dodecanol solution,
It is put into a side shoot of Y-shaped smell sensing apparatus, another side shoot is compared with method with ethyl alcohol, and the female moth that will mate is from Y-piece base portion mouth
It is put into, 10 every time, the crape of 2 mm apertures of apparatus seals anti-adult and escapes from.Moth can crawl toward or fly to against the wind odor source, know
Other odor source is simultaneously made a response.Two side shoot of Y-piece is neutralized still in the worm that do not make a response in base portion when recording 30 min respectively
Number, from two side shoot intersections, 3 cm or more of advance are denoted as positive reaction.4.18,6.75 mg/mL solution are tested with method.Weight
Multiple 5 secondary surveys, are averaged, SPSS 17.0 does statistical analysis.The results are shown in Figure 5.
After often finishing a secondary survey, the direction of Y-piece and odor source is exchanged.After finishing test twice, by Y-piece and taste source
Bottle is removed, and is cleaned, disinfection, is dried, then does next secondary survey.Used female moth is not repeated to use.It is raw to survey in calm, room temperature 25
DEG C, shading, under the conditions of air humidity 75%, at night 7: 00 ~ 12:The period of adult frequent activity carries out between 00.From base portion
To odor source, dodecanol forms ascending concentration gradient for inlet, and beet armyworm mates female moth energy identification information chemical combination
Species and its concentration gradient, and make corresponding reaction, i.e., in nature beet armyworm identification infected powdery mildew
The principle of host is consistent.
As shown in Figure 5, in Olfactory behavior test, dodecanol shows that apparent repellent is made to beet armyworm female adult
With.When mass concentration is 2.31mg/mL, scattering ratio is maximum, up to 42.9%.
Embodiment 4：Dodecanol tests the repellent action of oviposition behavior
About 3 cm thickness sterilized soils, appropriate amounts of sterilized water moistening are padded in 2 500 mL beakers.By two beams, each 5 are about 20 cm, leaf
The fresh Chinese Chinese rose branch (not using susceptible branch) of the identical healthy no disease and pests harm of base sheet, is inserted into 2 beakers respectively
In interior soil, two beakers are respectively labeled as 1, No. 2.It is molten that dodecanol is configured to 2.31 mg/mL with ethanol as solvent
Liquid takes the 5 mL solution uniformly to spray the branches and leaves in No. 1 beaker, another to spray the work of the branches and leaves in No. 2 beakers with method with 5 mL ethyl alcohol
Control.Two beakers are put into made of thin wire gauze in cage of laying eggs, at a distance of about 50 cm.
Artificial feeding beet armyworm distinguishes male and female to pupating, by pupa taking-up under stereoscope, and male and female pupa separates individually raising
To emergence.It selects 7 pairs sprout wings on the same day, develop normal, male and female pairing beet armyworm adult and be put into cage and lay eggs, on branch
Suspension is moistened with the cotton of proper honey water (10%), as adult foods source.Per the total egg laying amount and ovum on two kinds of branches of day entry
Block number to all female moths no longer lay eggs.After checking oviposition quantity every time, replaces fresh braches and spray dodecanol solution and nothing
Water-ethanol.Room temperature, shading, air humidity 75%.It is repeated 5 times, SPSS 17.0 does statistical analysis.Other two concentration (4.18,6.75
Mg/mL dodecanol solution) is tested with method.As a result as shown in Fig. 6 and table 2.
Influence (mean ± SD) of 2 dodecanol of table to pieces of an egg number (block)
Note：Different lowercase letters dodecanol and ethanol control in same mass concentration, same time（CK）Compared to difference
It is different significantly (P≤ 0.05)；Different capitalizations indicate differences reach the pole level of signifiance (P≤ 0.01).Scattering ratio is according to sweet tea
The pieces of an egg number total amount (Total) of dish noctuid calculates, and formula is：RR(%)=(N CK N T)/(N CK + N T)*100%, whereinRRFor
Scattering ratio（repellent rate, %）,N CKFor total pieces of an egg number of control group,N TFor total pieces of an egg number of dodecanol processing group.
The results show that either from pieces of an egg number still from the point of view of egg laying amount, dodecanol produces beet armyworm for Fig. 6 and table 2
Select to ovum fairly obvious repellent action.When mass concentration is 2.31 mg/mL, repellent effect is best.By being produced in Fig. 6
Ovum amount total value calculates, and maximum scattering ratio is 46.9%.Dodecanol mass concentration is can be seen that in conjunction with Olfactory behavior test result
In 4.18 mg/mL, to beet armyworm oviposition and Olfactory behavior inhibiting effect it is stronger, after increase with concentration, scattering ratio is not
It obviously increases, therefore optimal use concentration is advisable with 4.18 mg/ml.
Embodiment 5：Field experiment
The field experiment for the repellent activity that dodecanol lays eggs to beet armyworm is in Yunnan Province Kunming Chenggong area rose plant base
It is carried out in the greenhouse on ground.Experiment starts not using any pesticide within preceding 10 d, carries out normal water and fertilizer management.In when experiment starts
State Chinese rose is averaged 50 cm of plant height, processing cell and each 4 m of check plot area2, it is spaced 2 m.Wherein, every plant of use of cell is handled
1 mL of dodecanol solution of 4.18mg/mL sprays Chinese Chinese rose complete stool, and check plot is only sprayed with the ethyl alcohol of same volume with method.
It is put into the beet armyworm adult of the male and female pairing of 10 pairs of artificial feedings in greenhouse, collects daily, count worm's ovum amount and pieces of an egg number,
Total egg laying amount and total pieces of an egg number are counted after 5 d.3 repetitions are set per concentration.Humidity 75%~95% in greenhouse, 18~40 DEG C of temperature,
Other method and conditions are identical as indoor egg laying amount test experiments.Without work such as the disinfections that carries out soil and plant, as a result such as
Shown in table 3.
It can be seen that in greenhouse under rose plant actual production conditions from the total value of pieces of an egg number and egg laying amount in table 3, ten
Dialkanol also shows that the apparent repellent action laid eggs to beet armyworm.From the point of view of egg laying amount, scattering ratio is up to 36.8%.
Dodecanol is similarly obtained verification to the repellent action of the female moth of beet armyworm in field experiment.Dodecane is added in repellant
Base benzene sulfonic acid sodium salt is not found significant difference from the point of view of anthelminthic effect, but is that it increases the dissolving of dodecanol in water
Degree, thus can use water as solvent in actual use.
3 dodecanol repellent activity field experiment verification result of table
aFour kinds of data are the result calculated according to ethanol control.
In conclusion dodecanol can play effective regulating and controlling effect to beet armyworm Oviposition site selection behavior, if
Beet armyworm detects the presence of dodecanol, then can avoid laying eggs on the plant, in beet armyworm Oviposition site selection process
In play a key effect, can by the semiochemical for the pests such as beet armyworm non-environmental pollution control, have a extensive future.
Embodiment 6
A kind of insects repellant, including dodecanol, ethyl alcohol.The quality for the solution that the dodecanol is formulated with solvent
A concentration of 3.00mg/mL.The preparation method of the insects repellant is accurately to weigh 3.00 g dodecanols, molten with solvent
Solution is uniformly mixed, is settled to 1 L using solvent, obtains the insects repellant.
The application method of the insects repellant, after the insects repellant is diluted 0 times with ethyl alcohol, Direct Uniform sprinkling
On the bud and branches and leaves of plant.The beet armyworm is the female moth of beet armyworm.
The scattering ratio of the present embodiment is 30%, it may thus be appreciated that dodecanol can play beet armyworm Oviposition site selection behavior
To effective regulating and controlling effect.
Embodiment 7
A kind of insects repellant, including dodecanol, methanol.The quality for the solution that the dodecanol is formulated with solvent
A concentration of 5.00mg/mL.The preparation method of the insects repellant is accurately to weigh 5.00 g dodecanols, molten with solvent
Solution is uniformly mixed, is settled to 1 L using solvent, obtains the insects repellant.
The application method of the insects repellant, after the insects repellant is diluted 5 times with ethyl alcohol, Direct Uniform sprinkling
On the bud and branches and leaves of plant.The beet armyworm is the female moth of beet armyworm.
The scattering ratio of the present embodiment is 29.2%, it may thus be appreciated that dodecanol can be to beet armyworm Oviposition site selection behavior
Play effective regulating and controlling effect.
Embodiment 8
A kind of insects repellant, including dodecanol, ethyl alcohol, neopelex.The dodecanol is matched with solvent
The mass concentration of solution made of system is 4.50mg/mL.The preparation method of the insects repellant includes（1）Mother liquor A's matches
System：4.50 g dodecanols accurately are weighed, are dissolved with solvent；（2）Assist the preparation of liquid B：Mass fraction is prepared with deionized water
For 10% neopelex solution；（3）10mL B solutions are taken to be mixed with solution A, 5 min mixings of ultrasonic wave；（4）Make
It is settled to 1 L with solvent, obtains the insects repellant.
The application method of the insects repellant, after the insects repellant is diluted with water 2 times, Direct Uniform is sprayed at
On the bud and branches and leaves of plant.The pest is bollworm.
The scattering ratio of the present embodiment is 32.3%, is made it may thus be appreciated that dodecanol can play bollworm effective regulation and control
With.
Claims (10)
1. a kind of insects repellant, which is characterized in that include dodecanol.
2. insects repellant according to claim 1, which is characterized in that also include solvent.
3. insects repellant according to claim 2, which is characterized in that the solvent is in ethyl alcohol, methanol, n-hexane
It is one or more.
4. insects repellant according to claim 3, which is characterized in that the dodecanol was formulated with solvent
The mass concentration of solution is 2.31~6.75 mg/mL.
5. insects repellant according to claim 4, which is characterized in that the mass concentration of the solution is 4.18mg/mL.
6. according to any insects repellant of claim 1 ~ 5, which is characterized in that contain neopelex.
7. a kind of preparation method of insects repellant according to claim 6, which is characterized in that include the following steps：
（1）The preparation of mother liquor A：2.31~6.75g dodecanols accurately are weighed, are dissolved with solvent；
（2）Assist the preparation of liquid B：The neopelex solution that mass fraction is 10% is prepared with deionized water；
（3）10mL B solutions are taken to be mixed with solution A, 4 ~ 6 min mixings of ultrasonic wave；
（4）It is settled to 1 L using solvent, obtains the insects repellant.
8. a kind of application method of insects repellant according to claims 1 to 6, which is characterized in that drive the pest
After keeping away 0 ~ 5 times of agent ethyl alcohol or water dilution, Direct Uniform is sprayed on the bud and branches and leaves of plant.
9. insects repellant according to claim 1, which is characterized in that the pest is beet armyworm and/or cotton boll
Worm.
10. insects repellant according to claim 9, which is characterized in that the beet armyworm is the female moth of beet armyworm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810400895.6A CN108378030A (en) | 2018-04-28 | 2018-04-28 | A kind of insects repellant and its preparation and application method containing dodecanol |
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| CN201810400895.6A CN108378030A (en) | 2018-04-28 | 2018-04-28 | A kind of insects repellant and its preparation and application method containing dodecanol |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110308225A (en) * | 2019-07-26 | 2019-10-08 | 西南林业大学 | A kind of application of tetradecanol |
| CN110346476A (en) * | 2019-07-26 | 2019-10-18 | 西南林业大学 | A kind of application of dodecanol |
| CN110720458A (en) * | 2019-11-06 | 2020-01-24 | 西南林业大学 | Application of 2-decanone |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093221A (en) * | 1992-08-18 | 1994-10-12 | 以色列农业部农业研究组织博尔坎中心 | The arthropod control composition that is used for plant protection |
| CN1103755A (en) * | 1993-10-01 | 1995-06-21 | 林恩·休·詹姆斯 | Insecticide and insect repellant compositions |
| CN102396532A (en) * | 2010-09-09 | 2012-04-04 | 黄葛明 | High-efficiency insect repellant |
| CN105961387A (en) * | 2016-05-16 | 2016-09-28 | 西南林业大学 | Method employing hexadecanoic acid butyl ester as female spodoptera exigua hiibner attractant |
| CN106818743A (en) * | 2017-02-27 | 2017-06-13 | 西南林业大学 | A kind of insects repellant and its preparation and application |
-
2018
- 2018-04-28 CN CN201810400895.6A patent/CN108378030A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093221A (en) * | 1992-08-18 | 1994-10-12 | 以色列农业部农业研究组织博尔坎中心 | The arthropod control composition that is used for plant protection |
| CN1103755A (en) * | 1993-10-01 | 1995-06-21 | 林恩·休·詹姆斯 | Insecticide and insect repellant compositions |
| CN102396532A (en) * | 2010-09-09 | 2012-04-04 | 黄葛明 | High-efficiency insect repellant |
| CN105961387A (en) * | 2016-05-16 | 2016-09-28 | 西南林业大学 | Method employing hexadecanoic acid butyl ester as female spodoptera exigua hiibner attractant |
| CN106818743A (en) * | 2017-02-27 | 2017-06-13 | 西南林业大学 | A kind of insects repellant and its preparation and application |
Non-Patent Citations (2)
| Title |
|---|
| 杨发忠等: "中国月季感染白粉菌后挥发性成分对甜菜夜蛾嗅觉行为的影响", 《河南农业科学》 * |
| 杨文学等: "十八烷酸丁酯对甜菜夜蛾产卵和嗅觉行为的影响", 《河南农业科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110308225A (en) * | 2019-07-26 | 2019-10-08 | 西南林业大学 | A kind of application of tetradecanol |
| CN110346476A (en) * | 2019-07-26 | 2019-10-18 | 西南林业大学 | A kind of application of dodecanol |
| CN110720458A (en) * | 2019-11-06 | 2020-01-24 | 西南林业大学 | Application of 2-decanone |
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Application publication date: 20180810 |