CN108293996A - A kind of insects repellant and its preparation and application method containing methyl myristate - Google Patents
A kind of insects repellant and its preparation and application method containing methyl myristate Download PDFInfo
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- CN108293996A CN108293996A CN201810402586.2A CN201810402586A CN108293996A CN 108293996 A CN108293996 A CN 108293996A CN 201810402586 A CN201810402586 A CN 201810402586A CN 108293996 A CN108293996 A CN 108293996A
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- insects repellant
- beet armyworm
- methyl myristate
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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Abstract
The invention discloses a kind of insects repellant containing methyl myristate and its preparation and application method, the insects repellant includes methyl myristate, can also add the auxiliary elements such as ethyl alcohol equal solvent and neopelex.The repellant has apparent repellent action to pests such as beet armyworms.Also, the repellant main component is Secondary metabolites, can degrade in nature, be a kind of non-harmful insects repellant, have broad application prospects.
Description
Technical field
The invention belongs to technical field of plant protection, further belong to biological pesticide Prevention Technique field, and in particular to one
Insects repellant and its preparation and application of the kind containing methyl myristate.
Background technology
As modern agriculture continues to develop, more it is rooted in the hearts of the people to the protection of ecological environment.At the same time, plant protection is managed
It reads and continues to develop variation, known together with " anti-" first to become industry during pest control, rather than harmful organism outburst is
" controlling ", therefore, botanical pesticide comes into being, and the solution for " 3R " problem existing for conventional chemical pesticide provides new way.
Botanical pesticide has diverse biological activities (desinsection, sterilization, weeding and the mode of action are varied), noresidue, pacifies to environment
Entirely, the advantages that being not likely to produce drug resistance to higher mammal and natural enemies security, pest has become the hot spot of people's research at present.This
Wherein, the screening of botanical attractant and repellant causes experts and scholars' highest attention.Attractant and repellant are as information
Compound plays direct key effect during agriculture and forestry injurious insect identifies host, and the concentration of semiochemical is only in air
Need to reach nanogram level or it is lower can effectively be identified by insect, therefore, this kind of semiochemical be used for harmful insect prevention,
Effectively insect lipids can be regulated and controled, have the characteristics that preventive effect is high.In addition, since insect often has learning ability, when
Insect is attracted to destination, finds that no host plant exists, then attractant effect can decline, so the use of attractant exists
Certain risk.And repellant is then different, can effectively avoid the above situation.Certainly, if there is enough attractants and
The type of repellant then can use the two, for the prevention of agriculture and forestry injurious insect simultaneously, to form push-pull system, keep preventive effect aobvious
It writes and improves.Due to the previous more screenings for focusing on attractant of research, more achievement is had been achieved for, large quantities of activity are had accumulated
Preferable compound, therefore, just at present, the screening of repellant seems more urgent.
Have it is more studies have shown that obligatory parasitism fungal infection host plant the insect on same host can be caused it is unfavorable
It influences.Beet armywormSpodoptera exigua(H ü bner) belongs to Lepidoptera, Noctuidae, is a kind of worldwide obstinate pest,
Especially Chinese Chinese rose（That is rose）In greenhouse production process, beet armyworm harm is extremely serious.
For this purpose, develop it is a kind of using plant pathogenic fungi induction host plant synthesis semiochemical be mainly at
The insects repellant divided is used for the prevention of beet armyworm, has a very important significance.
Invention content
An object of the present disclosure is to provide a kind of nuisanceless insects repellant of plant source；Second be designed to provide it is described
The preparation method of insects repellant；Third is designed to provide the application method of the repellant.
What an object of the present disclosure was achieved, the insects repellant includes methyl myristate.
What the second purpose of the invention was achieved, the preparation method of the insects repellant includes the following steps：
（1）The preparation of mother liquor A：0.87~8.70 g methyl myristates accurately are weighed, are dissolved with solvent；
（2）Assist the preparation of liquid B：The neopelex solution that mass fraction is 10% is prepared with deionized water；
（3）10mL B solutions are taken to be mixed with solution A, 4 ~ 6 min mixings of ultrasonic wave；
（4）It is settled to 1 L using solvent, obtains the insects repellant.
What third purpose of the present invention was achieved, the application method of the insects repellant is to use the insects repellant
After ethyl alcohol or water dilute 0 ~ 5 times, Direct Uniform is sprayed on the bud and branches and leaves of plant.
Compared with prior art, beneficial effects of the present invention：
1, the insects repellant containing methyl myristate of the invention can make beet armyworm adult pass through the nasal receptor of oneself
Device identifies these compounds, will avoid laying eggs on the plant, therefore can prevent harm of the worm to plant, evil of the invention
Prevention of the worm repellant due to being used directly for female moth, it is therefore more efficient, it avoids because there are single-female generations for beet armyworm
Phenomenon and the prevention that brings is difficult.
2, the present invention is tested by Olfactory behavior biological test and field oviposition it is found that methyl myristate is to pest, spy
Not being beet armyworm female adult has good repellent effect.And since methyl myristate is natural secondary metabolite,
Nature has complete degradation pathway；Therefore, insects repellant is made using methyl myristate as main component, to realize
The non-environmental pollution control of pest.
Description of the drawings
Powdery mildew infects the influence to beet armyworm egg laying amount in the experiment of Fig. 1 single choices；
Powdery mildew infects the influence to beet armyworm egg laying amount in the bis- choosing experiments of Fig. 2；
Fig. 3 beet armyworms female moth that do not mate reacts the GC-EAD of susceptible Chinese Chinese rose volatile ingredient；
Fig. 4 beet armyworms female moth that mates reacts the GC-EAD of susceptible Chinese Chinese rose volatile ingredient；
Fig. 5 different quality concentration methyl myristates mate to beet armyworm the influence of female moth Olfactory behavior；
In figure, the calculation formula of scattering ratio is in Olfactory behavior biological test
RR(%)=(N CK N T)/(N CK + N T) × 100%,
Wherein,RRFor scattering ratio (repellent rate),N CKFor the female moth number of ethanol control side,N TFor methyl myristate
The female moth number of side,N NFor unreacted borer population.Using χ2Examine it is for statistical analysis,P<0.05 indicates significant difference；
Inhibiting effect of Fig. 6 methyl myristates to beet armyworm egg laying amount；
In figure, each mass concentration of methyl myristate is poor compared with control (CK) respectively in the different lowercase letter same periods
It is different significantly (P≤0.05)；Different capitalizations indicate compared with compareing (CK) differences reach the pole level of signifiance (P≤0.01)。
Specific implementation mode
With reference to embodiment and attached drawing, the invention will be further described, but is not limited in any way the present invention
System each falls within the scope of the present invention based on any transformation made by the present invention.
A kind of insects repellant of the present invention includes methyl myristate.Tetradecanoic acid first can be used directly in the present invention
Ester can also add solvent and other auxiliary elements as repellant.The solvent is one in ethyl alcohol, methanol, n-hexane
Kind is a variety of.In addition, auxiliary element such as neopelex etc. can be added, neopelex does not interfere with 14
The anthelminthic effect of alkanoic acid methyl esters, but the solubility of methyl myristate in water can be increased.In actual use, 12 are added
The insects repellant of sodium alkyl benzene sulfonate can be diluted with water.
Further include surfactant in the insects repellant, the surfactant is in pest repelling agent solution
Solubilising, dispersion are can aid in, while improving pest repelling agent solution in use on the bud and branches and leaves of plant
Spreadability, enhancing effect.
Preferably, the solvent is ethyl alcohol to one kind as the present invention.The solution that methyl myristate is formulated with ethyl alcohol
Mass concentration be 0.87~8.70 mg/mL.
Preferably, the mass concentration of the solution is 4.35mg/mL to one kind as the present invention.
A kind of preparation method of insects repellant, includes the following steps：
（1）The preparation of mother liquor A：0.87~8.70 g methyl myristates accurately are weighed, are dissolved with solvent；
（2）Assist the preparation of liquid B：The neopelex solution that mass fraction is 10% is prepared with deionized water；
（3）10mL B solutions are taken to be mixed with solution A, 4 ~ 6 min mixings of ultrasonic wave；
（4）It is settled to 1 L using solvent, obtains the insects repellant.
The application method of the insects repellant is after the insects repellant ethyl alcohol or water are diluted 0 ~ 5 times, directly
It connects and is uniformly sprayed on the bud and branches and leaves of plant.
The pest is beet armyworm and/or bollworm.
The beet armyworm is the female moth of beet armyworm.
Insects repellant of the present invention can be used for the prevention of the pests such as beet armyworm, bollworm, be driven to beet armyworm
Avoiding effect is best, can especially be used for the most important feature that the female moth of repellent beet armyworm oogenesis is the repellant.
The operation principle of the present invention：
Chinese Chinese rose（Rosa chinensisJacq., it is commonly called as rose）It is one of four big Fresh Cutting flower of the world, it is deep to be liked by people
Love, is widely cultivated in the whole world.But the pest and disease damage generation on Chinese Chinese rose is also extremely serious, includes mainly Sphaerotheca pannosaSphaerotheca pannosa(Wallroth ex Fries) L é veill é, beet armywormSpodoptera exigua
(H ü bner) etc..Some researches show that there may be advantageous or detrimental effects to insect pest for disease, and in disease pest Interaction
In, volatile component of plant and its variation play a crucial role the Tropical area of insect.Powdery mildew infects the Chinese moon
Season has a small amount of report to the research that beet armyworm impacts, it has proved that powdery mildew will produce beet armyworm unfavorable shadow
It rings.The volatile ingredient for the Chinese rose that the healthy Chinese rose of extraction and powdery mildew infect respectively is used to test living to the biology of beet armyworm
Property, the results showed that, healthy Chinese rose volatile ingredient shows apparent attracting action to beet armyworm adult；Susceptible Chinese rose volatility
Ingredient then shows apparent repellent action to beet armyworm adult.Using GC-EAD methods further to susceptible Chinese rose volatility at
Divide and carry out separation identification, it is found that methyl myristate therein is the important activity ingredient for generating repellent action.
The features of the present invention：Information chemical combination of the methyl myristate as a kind of beet armyworm susceptible host for identification
Object will not generate Chinese rose plant harmful effect, will not lead to the worm for being prevented beet armyworm instead of chemical pesticide
It develops immunity to drugs.Meanwhile methyl myristate comes from host plant, there is natural complete degradation pathway in nature,
Harmful effect will not be generated to environment, be a kind of environmentally friendly active constituent, have broad application prospects.
Embodiment 1：The discovery of pest repelling substance
(1) beet armyworm method for breeding
The beet armyworm mature larva adopted back is put into the 250 mL beakers for being lined with moist sandy soil, is sealed with gauze, wait pupating,
After emergence, adult is transferred in another 250 mL beaker.It is supplemented the nutrients with 10% hydromel, makes it in Health China Chinese rose
It lays eggs, hatch on blade, and raise to mature larva (rearing conditions：27 DEG C, 70% RH, 12 h photoperiods).It is allowed to change with method
Pupa taking-up is distinguished male and female by pupa under stereoscope, and male and female pupa is separately raised, and biological test is used for after emergence.
(2) inhibiting effect of the host plant infection powdery mildew to beet armyworm Adult worms producting eggs behavior
About 3 cm thickness sterilized soils are padded in 2 500 ml beakers, spill appropriate amounts of sterilized water moist soil.It is new to choose Health China Chinese rose
Each 6 of the Chinese Chinese rose fresh braches (about long 30 cm of branch) of fresh branch and infection powdery mildew, are inserted into 2 beakers respectively
In soil, cannot occur a large amount of liquid waters in beaker, to prevent moth is overboard to be drowned.And the beaker for being plugged branch is respectively put into
In insect cage made of 2 identical wire nettings, black cloth shading.Picked out from the 1 day adult of sprouting wings 8 pairs circle in the air it is normal, female
The beet armyworm adult of hero pairing is put into cage, and the cotton for being moistened with appropriate 10% hydromel, the battalion as adult are hung on branch
The source of supporting.Egg laying amount on later next day observation branch replaces fresh braches after checking oviposition quantity every time.4 days adults after emergence
Start to lay eggs, the 4th, 6,8 day egg laying amount of statistics.It is repeated 4 times.
Health China Chinese rose fresh braches and susceptible Chinese each 6 of Chinese rose fresh braches (about long 30 cm of branch) are chosen, are adopted
It is put into beaker with method identical with single choice biological test and two beakers is placed in simultaneously in same insect cage, diagonally put
It sets, adult, the total egg laying amount of statistics of the pairing of 4 pairs of male and female is accessed with method, is repeated 4 times.The double choosing experiments of egg laying amount use identical side
Method is respectively in three different places（Chenggong, Kunyang and school experiment room, in Chinese Chinese rose planting greenhouse）It carries out, purpose
It is to check whether to affect experimental result because of the particularity of region, expected result has universality, to prevent in reality
It is merely a special case to test the result obtained in room only.
(3) result
Egg laying amount single choice biological test experimental result is as shown in Figure 1.All in all, in single choice biological test, the 4th after emergence
It is all higher than the egg laying amount on susceptible branch to eggs on average amount of the 8th day beet armyworm on Health China Chinese rose branch.It removes
4th day egg laying amount difference is not significantly outer, the 6th day and the 8th day significant difference (P ＜ 0.05).It takes temperature from always, on healthy branch
673.3 ± 41.2；It it is 353.5 ± 33.5 on susceptible branch, difference reaches the pole level of signifiance.
On two kinds of Chinese Chinese rose branches, the egg laying amount of (2 days) is significantly greater in first period, is more than second and third
Egg laying amount in period, extension of the egg laying amount on two kinds of branches all at any time and reduce.On Health China Chinese rose branch, production
The degree of the extension at any time of ovum amount and reduction is not very big.But on susceptible Chinese Chinese rose, the degree of reduction is very big.Feeling
In the single choice biological test carried out on sick China's Chinese rose branch, there is more ovum production on oviposition cage inner wall, and in Health China
On Chinese rose branch, it there is no and observe the upper oviposition on the inside of oviposition cage, but concentrate on laying eggs on blade or on stem.
Double to select biological test the results are shown in Figure 2, the female moth of beet armyworm can freely select in health or sense in experiment
It lays eggs on the Chinese Chinese rose branch of disease.The result shows that beet armyworm is more willing to produce on Health China Chinese rose in double choosings test
Ovum.Total egg laying amount average out to 469.33 of the beet armyworm on Chinese Chinese rose health branch in minute under the conditions of double choosings ±
45.62/worm, the egg laying amount on the Chinese Chinese rose branch after infecting powdery mildew is 166.27 ± 31.52, is produced after susceptible
Ovum amount reduces 64.60%.Per day egg laying amount of the beet armyworm on Health China Chinese rose branch is obviously higher than susceptible China
Per day egg laying amount on Chinese rose branch, and difference is extremely notable（P < 0.01）.It finds simultaneously, beet armyworm is seldom in cage
It lays eggs on wall, it is different when from single choice biological test, show that female moth can be easily in the dark in unsoundness China Chinese rose branch
Identify their host in ground.
The result obtained from Kunyang, the different place in Chenggong and school experiment room three is almost the same, the difference between region
It is different not notable.
In experiment, healthy branch is compared with susceptible branch, and the various non-life conditions including morphology are all consistent
, but beet armyworm can therefrom identify susceptible branch and avoid oviposition, the growth and development wound for its offspring on susceptible branch
Make good condition.During Oviposition site selection, two kinds of unique difference of branch are that volatile ingredient difference, Noctuidae elder brother
Worm beet armyworm can only be identified host by the difference of the two volatile ingredient, thus should the result shows that, Chinese Chinese rose is waved
Hair property ingredient in beet armyworm Adult worms producting eggs plays particularly important effect in selection course, and Chinese rose plant infects powdery mildew
Volatile ingredient afterwards then shows obvious inhibiting effect to beet armyworm adult.
Embodiment 2：The separation and identification of repellent activity ingredient
The volatile ingredient mixture that the Chinese Chinese rose blade of infection powdery mildew is extracted using head space dynamic adsorption method, is used in combination gas phase
Chromatography-electrophysiology combined instrument (GC-EAD) carries out analysis test to the mixture, as a result as shown in Fig. 3, Fig. 4, table 1.It is right
The monomeric compound that can generate apparent EAD signals in mixture is further identified with GC-MS.Wherein, No. 3 peaks are the tetradecane
Sour methyl esters, the content of the compound in the mixture is larger, the EAD signals of generation are stronger.The result shows that from susceptible Chinese rose plant
Middle to extract obtained native compound (mixture), wherein containing methyl myristate (monomer), it may be that beet armyworm is female
Moth identifies one of important information compound of susceptible host during Oviposition site selection.
Table 1 causes the female moth of beet armyworm to generate the susceptible Chinese Chinese rose volatile ingredient that EAD reacts
Table 1 The volatiles from healthy rose plants with activity to induce
EAD responses of unmated female moths of beet armyworm
Note：" retention time " refers to the retention time in GC-MS." serial number " is corresponding with the number at peak in Fig. 2, Fig. 3.
Retention time in the table is from GC-MS spectrum. The peak numbers
correspond with the numbers in Figure 2 and 3.
Embodiment 3：Olfactory behavior of the beet armyworm female adult to methyl myristate
Key instrument used is Y-shaped smell sensing apparatus.22 cm of Y-piece major branch therein, two side shoots are 17 cm, and internal diameter is 5
Cm, 75 degree of two-arm angle.Y-piece is cleaned, is dried after being sterilized with absolute ethyl alcohol.By Y-shaped smell sensing apparatus by air pump, activated carbon,
Distilled water, LZB-II glass rotameters, three-way pipe, Y-piece sequence are connected with silicone tube, are horizontally arranged, black cloth shading, are adjusted
Solar term flow is 300 mL/min.Air-flow is intersected in Y-piece major branch, is flowed out from base portion mouth.Experiment is first passed through clean sky before starting
Gas 10 minutes.
In order to make gas slowly overflow, by the foraminate small glass of band equipped with 0.87mg/mL methyl myristate solution
Bottle, is put into a side shoot of Y-shaped smell sensing apparatus, and another side shoot is compared with method with ethyl alcohol, and the female moth that will mate is from Y-piece base portion
Mouth is put into, 10 every time, and the crape of 2 mm apertures of apparatus seals anti-adult and escapes from.Moth can crawl toward or fly to against the wind odor source,
Identification odor source is simultaneously made a response.Y-piece two side shoot is neutralized still in not making a response in base portion when recording 30 min respectively
Borer population, from two side shoot intersections, 3 cm or more of advance are denoted as positive reaction.4.35,8.70 mg/mL solution are tested with method.
It is repeated 5 times raw survey, is averaged, SPSS 17.0 does statistical analysis.The results are shown in Figure 5.
After often finishing a secondary survey, the direction of Y-piece and odor source is exchanged.After finishing test twice, by Y-piece and taste source
Bottle is removed, and is cleaned, disinfection, is dried, then does next secondary survey.Used female moth is not repeated to use.It is raw to survey in calm, room temperature 25
DEG C, shading, under the conditions of air humidity 75%, at night 7: 00 ~ 12:The period of adult frequent activity carries out between 00.From base portion
To odor source, methyl myristate forms ascending concentration gradient for inlet, and beet armyworm mates female moth energy identification information
Classes of compounds and its concentration gradient, and make corresponding reaction, i.e., in nature beet armyworm identification infected white powder
The principle of the host of bacterium is consistent.
As shown in Figure 5, in Olfactory behavior test, methyl myristate shows apparent repellent to beet armyworm female adult
Effect.When mass concentration is 8.70 mg/mL, scattering ratio is maximum, up to 29.4%.
Embodiment 4：Methyl myristate tests the repellent action of oviposition behavior
About 3 cm thickness sterilized soils, appropriate amounts of sterilized water moistening are padded in 2 500 mL beakers.By two beams, each 5 are about 20 cm, leaf
The fresh Chinese Chinese rose branch (not using susceptible branch) of the identical healthy no disease and pests harm of base sheet, is inserted into 2 beakers respectively
In interior soil, two beakers are respectively labeled as 1, No. 2.Methyl myristate is configured to 0.87mg/mL with ethanol as solvent
Solution takes the 5 mL solution uniformly to spray the branches and leaves in No. 1 beaker, separately sprays the branches and leaves in No. 2 beakers with method with 5 mL ethyl alcohol
It compares.Two beakers are put into made of thin wire gauze in cage of laying eggs, at a distance of about 50 cm.
Artificial feeding beet armyworm distinguishes male and female to pupating, by pupa taking-up under stereoscope, and male and female pupa separates individually raising
To emergence.It selects 7 pairs sprout wings on the same day, develop normal, male and female pairing beet armyworm adult and be put into cage and lay eggs, on branch
Suspension is moistened with the cotton of proper honey water (10%), as adult foods source.Per the total egg laying amount and ovum on two kinds of branches of day entry
Block number to all female moths no longer lay eggs.After checking oviposition quantity every time, replaces fresh braches and spray methyl myristate solution
And absolute ethyl alcohol.Room temperature, shading, air humidity 75%.It is repeated 5 times, SPSS 17.0 does statistical analysis.Other two concentration (4.35,
8.70 mg/mL) methyl myristate solution with method test.As a result as shown in Fig. 6 and table 2.
Influence (mean ± SD) of 2 methyl myristate of table to pieces of an egg number (block)
Note：Different lowercase letters methyl myristate and ethanol control in same mass concentration, same time（CK）Phase
Than significant difference (P≤ 0.05)；Different capitalizations indicate differences reach the pole level of signifiance (P≤ 0.01).Scattering ratio root
It is calculated according to the pieces of an egg number total amount (Total) of beet armyworm, formula is：RR(%)=(N CK N T)/(N CK + N T)*100%, whereinRRFor scattering ratio（repellent rate, %）,N CKFor total pieces of an egg number of control group,N TFor the total of methyl myristate processing group
Pieces of an egg number.
Fig. 6 and table 2 are the results show that either from pieces of an egg number still from the point of view of egg laying amount, and methyl myristate is to beet night
Select to moth egg fairly obvious repellent action.When mass concentration is 8.70mg/mL, repellent effect is best.In conjunction with smelling
Feel that performance testing result can be seen that methyl myristate mass concentration in 4.35mg/mL, to beet armyworm oviposition and smell
The inhibiting effect of behavior is stronger, after increase with concentration, scattering ratio does not obviously increase, therefore optimal use concentration is with 4.35 mg/ml
It is advisable.
Embodiment 5：Field experiment
The field experiment for the repellent activity that methyl myristate lays eggs to beet armyworm is in Yunnan Province Kunming Chenggong area rose kind
It is carried out in the greenhouse on phytyl ground.Experiment starts not using any pesticide within preceding 10 d, carries out normal water and fertilizer management.Experiment starts
Shi Zhongguo Chinese roses are averaged 50 cm of plant height, processing cell and each 4 m of check plot area2, it is spaced 2 m.Wherein, processing cell is every
Strain sprays Chinese Chinese rose complete stool with 1 mL of methyl myristate solution of 4.35 mg/mL, and the ethyl alcohol of same volume is only used in check plot
It is sprayed with method.It is put into the beet armyworm adult of the male and female pairing of 10 pairs of artificial feedings in greenhouse, collects daily, statistics worm's ovum amount
With pieces of an egg number, total egg laying amount and total pieces of an egg number are counted after 5 d.3 repetitions are set per concentration.Humidity 75%~95% in greenhouse, temperature
18~40 DEG C, other method and conditions are identical as indoor egg laying amount test experiments.Without works such as the disinfections that carries out soil and plant
Make, the results are shown in Table 3.
It can be seen that in greenhouse under rose plant actual production conditions from the total value of pieces of an egg number and egg laying amount in table 3, ten
Four alkanoic acid methyl esters also show that the apparent repellent action laid eggs to beet armyworm.Methyl myristate is to the female moth of beet armyworm
Repellent action verification is similarly obtained in field experiment.Neopelex is added in repellant, from anthelminthic effect
From the point of view of, it is not found significant difference, but be that it increases the solubility of methyl myristate in water, thus can actually make
It uses and uses water as solvent in the process.
3 methyl myristate repellent activity field experiment verification result of table
aFour kinds of data are the result calculated according to ethanol control.
In conclusion methyl myristate can play effective regulating and controlling effect to beet armyworm Oviposition site selection behavior,
It when beet armyworm detects the presence of methyl myristate, then can avoid laying eggs on the plant, be selected in beet armyworm spawning habitat
It plays a key effect during selecting, which can be used for non-environmental pollution control, the application prospect of the pests such as beet armyworm
It is wide.
Embodiment 6
A kind of insects repellant, including methyl myristate, ethyl alcohol.The methyl myristate is formulated molten with solvent
The mass concentration of liquid is 2.00mg/mL.The preparation method of the insects repellant is accurately to weigh 2.00 g tetradecanoic acid first
Ester is dissolved with solvent, is uniformly mixed, is settled to 1 L using solvent, obtains the insects repellant.
The application method of the insects repellant, after the insects repellant is diluted 0 times with ethyl alcohol, Direct Uniform spray
It is sprinkled upon on the bud and branches and leaves of plant.The beet armyworm is the female moth of beet armyworm.
The scattering ratio of the present embodiment is 19.1%, it may thus be appreciated that methyl myristate can be to beet armyworm Oviposition site selection
Effective regulating and controlling effect is played in behavior.
Embodiment 7
A kind of insects repellant, including methyl myristate, methanol.The methyl myristate is formulated molten with solvent
The mass concentration of liquid is 6.00mg/mL.The preparation method of the insects repellant is accurately to weigh 6.00 g tetradecanoic acid first
Ester is dissolved with solvent, is uniformly mixed, is settled to 1 L using solvent, obtains the insects repellant.
The application method of the insects repellant, after the insects repellant is diluted 5 times with ethyl alcohol, Direct Uniform spray
It is sprinkled upon on the bud and branches and leaves of plant.The beet armyworm is the female moth of beet armyworm.
The scattering ratio of the present embodiment is 22.3%, it may thus be appreciated that methyl myristate can be to beet armyworm Oviposition site selection
Effective regulating and controlling effect is played in behavior.
Embodiment 8
A kind of insects repellant, including methyl myristate, ethyl alcohol, neopelex.The methyl myristate
The mass concentration for the solution being formulated with solvent is 5.00mg/mL.The preparation method of the insects repellant includes（1）It is female
The preparation of liquid A：5.00 g methyl myristates accurately are weighed, are dissolved with solvent；（2）Assist the preparation of liquid B：Matched with deionized water
The neopelex solution that mass fraction processed is 10%；（3）10mL B solutions are taken to be mixed with solution A, ultrasonic wave 4 ~ 6
Min mixings；（4）It is settled to 1 L using solvent, obtains the insects repellant.
The application method of the insects repellant, after the insects repellant is diluted with water 2 times, Direct Uniform sprinkling
On the bud and branches and leaves of plant.The pest is bollworm.
The scattering ratio of the present embodiment is 21.5%, it may thus be appreciated that methyl myristate can play bollworm effective tune
Control acts on.
Claims (10)
1. a kind of insects repellant, which is characterized in that include methyl myristate.
2. insects repellant according to claim 1, which is characterized in that also include solvent.
3. insects repellant according to claim 2, which is characterized in that the solvent is in ethyl alcohol, methanol, n-hexane
It is one or more.
4. insects repellant according to claim 3, which is characterized in that the methyl myristate and solvent prepare and
At solution mass concentration be 0.87~8.70 mg/mL.
5. insects repellant according to claim 4, which is characterized in that the mass concentration of the solution is 4.35 mg/
mL。
6. according to any insects repellant of claim 1 ~ 5, which is characterized in that contain neopelex.
7. a kind of preparation method of insects repellant according to claim 6, which is characterized in that include the following steps：
（1）The preparation of mother liquor A：0.87~8.70 g methyl myristates accurately are weighed, are dissolved with solvent；
（2）Assist the preparation of liquid B：The neopelex solution that mass fraction is 10% is prepared with deionized water；
（3）10mL B solutions are taken to be mixed with solution A, 4 ~ 6 min mixings of ultrasonic wave；
（4）It is settled to 1 L using solvent, obtains the insects repellant.
8. a kind of application method of insects repellant according to claims 1 to 6, which is characterized in that drive the pest
After keeping away 0 ~ 5 times of agent ethyl alcohol or water dilution, Direct Uniform is sprayed on the bud and branches and leaves of plant.
9. insects repellant according to claim 1, which is characterized in that the pest is beet armyworm and/or cotton boll
Worm.
10. insects repellant according to claim 9, which is characterized in that the beet armyworm is the female moth of beet armyworm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110720458A (en) * | 2019-11-06 | 2020-01-24 | 西南林业大学 | Application of 2-decanone |
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