CN109042648B - Thrips attractant with benzyl alcohol and phenethyl alcohol as active ingredients and application thereof - Google Patents
Thrips attractant with benzyl alcohol and phenethyl alcohol as active ingredients and application thereof Download PDFInfo
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- CN109042648B CN109042648B CN201810885544.9A CN201810885544A CN109042648B CN 109042648 B CN109042648 B CN 109042648B CN 201810885544 A CN201810885544 A CN 201810885544A CN 109042648 B CN109042648 B CN 109042648B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/14—Catching by adhesive surfaces
- A01M1/145—Attracting and catching insects using combined illumination or colours and adhesive surfaces
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/04—Oxygen or sulfur attached to an aliphatic side-chain of a carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
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- Agronomy & Crop Science (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a thrips attractant, which comprises active ingredients of benzyl alcohol and phenethyl alcohol in a volume ratio of 2:8-5: 5. The thrips attractant provided by the invention is prepared by taking benzyl alcohol and phenethyl alcohol as main active ingredients and paraffin oil as a solvent, is matched with a pest sticking plate for use, has high pest trapping rate and obvious effect, and particularly has the best effect of trapping and killing thrips by the attractant with the volume concentration of the active ingredients of the benzyl alcohol and the phenethyl alcohol being 2.2:7.8 in volume ratio, lasting efficacy and synergistic effect; in addition, the benzyl alcohol and the phenethyl alcohol have low preparation cost and wide sources, are easy to popularize in a commercialized way and have good market prospect.
Description
Technical Field
The invention relates to the field of insect attractants, in particular to a thrips attractant taking benzyl alcohol and phenethyl alcohol as active ingredients and application thereof.
Background
Thrips is a world-wide spread multi-food pest belonging to Thrips (Thysanoptera) Thrips (Thripidae), and the presently recorded Thrips species in China belong to 336 species, while the Thrips species that are harmful to mangos are nearly 40 species, including Thrips virgatus dorsalis, Thrips flavus hawaiensis, Thrips Frankliniella intnsa, Thrips Frankliniella erythropolis, Thrips Seirtothrips rubra, Thrips virginiana, Thrips haplothinensis, Thrips fusciparus, Thrips fusus turssa, Thrips rhipifera, Thrips chromophilus, and the like, wherein the Thrips virginis, Thrips tea, Thrips, and the like are dominant species, Thrips virginiana, Thrips victorius, and mango tree, and the major pest control of the Thrips, and the Thrips damage during the period, particularly, is a outbreak prevention of the Thrips victorialis, and the Thrips victorialis is the most resistant to mango damage.
At present, the prevention and control of mango thrips mainly depend on pesticides, and the thrips have the characteristics of small size, easy concealment, strong reproductive capacity, high development speed, easy diffusion along with airflow and the like, so frequent pesticide application is needed, and the mango thrips are easy to generate pesticide resistance; in recent years, the use of highly toxic medicaments is limited for a plurality of reasons, so that the prevention and control cost is increased, an ideal effect is difficult to achieve, effective sustainable prevention and control is difficult to perform, and the yield and the quality of mangoes are seriously influenced.
Aiming at the current situation and the demand of mango thrips prevention and control, a sustainable thrips prevention and control method is found to be the basis for realizing green thrips prevention and control, pest monitoring and control are carried out by utilizing the chemotaxis of insects, and particularly, the prevention and control of banana flower thrips, western flower thrips and other species by utilizing plant volatile secondary substances, insect pheromones and the like are reported in research, for example, a reagent for inducing flower thrips is disclosed in patent CN201410607070.3, and the reagent takes (R) -lavender acetate and meat-based (S) -2-methylbutyrate or meat-based (S) -2-methylbutyrate analogues as main components and is used for inducing flower thrips; however, the active ingredients of the thrips attractant are not commercialized due to complex preparation process and high cost, so that the development of a new thrips attractant is beneficial to promoting the commercialization process and the application of the thrips attractant; the method realizes accurate monitoring of pests, thereby determining the optimal prevention and control time, making corresponding prevention and control measures, reducing the pesticide consumption, improving the prevention and control effect and delaying the generation of pesticide resistance of the pests to the pesticide.
Disclosure of Invention
In view of the above, the invention provides a thrips attractant, which solves the problems of poor thrips trapping and killing effect, high cost and the like in the prior art.
The invention provides a thrips attractant which takes benzyl alcohol and phenethyl alcohol as active ingredients.
Preferably, the volume ratio of the benzyl alcohol to the phenethyl alcohol is 2:8-5: 5.
Preferably, the volume ratio of benzyl alcohol to phenethyl alcohol is 2.2: 7.8.
Preferably, the attractant further comprises paraffin oil, and the paraffin oil accounts for 99-99.98% of the volume.
Preferably, the paraffin oil is 99.8% by volume.
On the other hand, the thrips attractant is used for monitoring, preventing and controlling thrips, and is specifically loaded into a centrifugal tube carrier to obtain an attraction core; lure the core to hug closely the mythimna separata board upper corner and hang together, pass centrifuging tube lid detain the hole and hang with the mythimna separata board hanging hole, lure the thrips, the centrifuging tube carrier is 1.5ml and covers the centrifuging tube even, opens a plurality of 0.3 mm's hole above the centrifuging tube lid.
The thrips attractant provided by the invention has good attraction effect on tea thrips and flower thrips; the bait is used in combination with yellow armyworm plate when trapping tea thrips, and is used in combination with blue armyworm plate when trapping flower thrips.
The thrips attractant provided by the invention is adopted to extract effective components from mango flowers, and is further screened, benzyl alcohol and phenethyl alcohol are used as main active components, paraffin oil is used as a solvent to be mixed to prepare the attractant, and the attractant is matched with a pest sticking plate for use, so that the thrips attractant has high pest-attracting rate and excellent effect, particularly, the attractant with the volume concentration of the active components of the benzyl alcohol and the phenethyl alcohol being 2.2:7.8 has the best effect of attracting and killing the thrips, and has rapid reaction and lasting effect, can obviously improve the attracting capability to the thrips, and has synergistic effect; in addition, the benzyl alcohol and the phenethyl alcohol have low preparation cost and wide sources and are easy to be popularized in a commercialized way.
Drawings
FIG. 1 is a schematic diagram showing the effect of the thrips attractant in the fourth embodiment on outdoor thrips trapping;
FIG. 2 is an enlarged schematic view of FIG. 1;
FIG. 3 shows the experimental results of different attractants for indoor attraction of Scutellaria pseudolaricis, the actual attraction rate is shown in series 1, and the theoretical attraction rate is shown in series 2;
FIG. 4 shows the results of 24h and 48h experiments with different attractants at 0.2% concentration for outdoor killing of thrips theae, wherein the attracting results for 24h are shown in series 1, and the attracting results for 48h are shown in series 2.
Detailed Description
The principles and features of this invention are described below in conjunction with examples which are set forth to illustrate, but are not to be construed to limit the scope of the invention.
The raw material products related to the invention, namely benzyl alcohol, phenethyl alcohol, alpha-terpineol, phenylacetaldehyde, verbenol and paraffin oil, are purchased from a regular reagent company, and the purity is not less than 99.5 percent.
The tea yellow thrips used in the invention is collected from mango seed nursery of research institute of tropical crop variety resources of the institute of tropical agricultural science of the academy of tropical agriculture of Hainan delirium and mango base of XiLing farm of the Hainan east island, the indoor temperature is 25 +/-3 ℃, thrips are raised in an insect cage of 50 x 50cm by taking mango tender leaves as food, and active adults are selected for indoor test.
Mango flower (Tainong) used in the invention is collected in mango seed orchard of research institute of tropical crop variety resources of tropical agricultural science institute of China, Hainan delirium.
Example one assay of the attractive Activity of mango flower volatile oil on thrips
The mangifera volatile oil is obtained by distilling and extracting through anhydrous ether.
The volatile secondary substances in the mango flower volatile oil comprise benzyl alcohol, phenethyl alcohol, alpha-terpineol, phenylacetaldehyde, verbenol and the like.
Measuring the tendency activity of female thrips on a sample to be measured by utilizing a Y-shaped olfactometer, sucking 50 mu L of liquid to be measured by using a liquid transfer gun, dripping the liquid to be measured on a filter paper strip, putting the filter paper strip which is dripped with 50u1 paraffin oil into a wide-mouth bottle connected with one side arm of a Y-shaped pipe to serve as an odor source, and putting the filter paper strip which is dripped with 50u1 paraffin oil into a wide-mouth bottle connected with the other side arm to serve as a contrast odor source; the flow rate of the air flow meter of both side arms was set to 60m 1/min. Releasing the single-headed thrips into the base of the Y-shaped tube, and observing the behavioral response of the adult within 3 min. When the insect crosses the 30mm position of the side test arm, recording as a test insect to select the odor source connected with the arm; if adult insects are not selected after 3min, no response is recorded. Each group of treatment tests 60 heads of female thrips, and each time 10 test heads of test insects are tested, a new Y-shaped pipe is replaced. The test is carried out in an environment of 25 + -3 deg.C. And cleaning the Y-shaped pipe, the flavor source bottle, the connecting rubber pipe and the like by using ethanol after the test is finished every day. The test insect selection results were counted and the luring rate was calculated as shown in table 1.
TABLE 1 indoor attractant activity assay of mango flower volatile oil on thrips theae
Example two-time indoor attraction activity measurement of different thrips attractants to thrips theae, benzyl alcohol, phenethyl alcohol, alpha-terpineol, phenylacetaldehyde and verbenol are respectively selected as active ingredients, and are mixed with paraffin oil according to the volume ratio of 1:99, 0.2:99.8 and 0.1:99.9 for preparation, then indoor tendency activity measurement is carried out according to the same method of example one, and attraction rates are calculated, as shown in table 2.
TABLE 2 measurement of the attractant activity of different thrips attractants against thrips Camellia
The experimental result shows that the benzyl alcohol, the phenethyl alcohol and the verbenol have good attracting effect on the tea yellow thrips, and the single-component attractant with the concentration of 1% has the attracting and killing capacity of 65% -75%, so the single-component attractant has the prospect of preparing the thrips attractant.
In the third embodiment, the indoor attraction activity of the tea thrips is measured by the active ingredient attractants with different proportions, the three active ingredients of benzyl alcohol, phenethyl alcohol and verbenol are mixed in pairs or three active ingredients are mixed to prepare the attractants, the attractants are mixed with paraffin oil according to different proportions, then the indoor tendency activity test of the tea thrips is carried out, and the attraction rate is calculated, as shown in table 3. Wherein the ratio of each active component in the attractant is multiplied by the trapping capacity of the component by theoretical calculation, such as the attractant (A: B ═ 1:9) ═ 1/10 xA +9/10 xB). times.100%.
TABLE 3 indoor attracting activity test results of different attractants to thrips theae
Notes A, benzyl alcohol, B, phenethyl alcohol and C verbenol (the same below)
As can be seen from the indoor attraction results shown in Table 3 and FIG. 3, when the volume ratio of benzyl alcohol to phenethyl alcohol is 2:8-5:5 and the volume concentration is 0.1% -1%, the benzyl alcohol and the phenethyl alcohol have relatively obvious attraction effect on the tea thistle, and compared with the superposition of the attraction and killing capability of each independent component, the benzyl alcohol and the phenethyl alcohol can be synergistic in a specific ratio, and the attraction effect of the benzyl alcohol and the phenethyl alcohol on the thrips can be complementary.
Example four experiments of outdoor trapping and killing of thrips tea with thrips attractant of different active ingredients, mango orchards planted with Tainong mango variety and with row spacing of about 3m x 5m were selected. Adopting a random block group design, totally arranging 5 repeating regions, hanging yellow sticky boards and attractants with different proportions in each repeating region by adopting an interlaced and plant-separated method, and loading 1.0ml of thrips attractant into a centrifugal tube carrier to obtain a lure core; lure the core to hug closely the mythimna separata board upper corner and hang the hole, pass centrifuging tube lid detain the hole and hang together with yellow mythimna separata board hanging hole, lure the thrips, the centrifuging tube carrier is 1.5ml even covers the centrifuging tube, opens a plurality of 0.3 mm's hole above the centrifuging tube lid, uses yellow mythimna separata board + paraffin oil as the control group simultaneously.
The number of theaflavins attracted per yellow armyworm plate was recorded 24 hours and 48 hours after suspending the yellow armyworm plate + the attracting substances of different active ingredients, and the number of the attracted theaflavins for each repetition zone was counted and the average value was calculated as shown in table 4.
TABLE 4 outdoor trapping and killing experiment results of different attractants for thrips theae
Remarking: the error of repeated treatment of the same experimental group is not more than 8%.
As can be seen from table 4 and fig. 4, compared to the paraffin oil control group, the insect-trapping ability of the attractant of the present invention is (number of insects trapped in the experimental group-number of insects trapped in the paraffin oil control group)/number of insects trapped in the control group, paraffin oil is used as the attractant, yellow plates are used to trap and kill thrips, 106 thrips are trapped and killed after 24h, 368 are trapped and killed after 48h, benzyl alcohol and phenethyl alcohol are used as the active ingredients, the highest number is 1230.4 after 24h, the highest number is 2268.8 after 48h, and the trapping and killing ability is increased by more than 6 times.
Example five, in an outdoor trapping experiment of thrips tea with different concentrations of thrips attractant, 1mL of benzyl alcohol and phenethyl alcohol active ingredients with the volume ratio of 2.2:7.8 are respectively added with paraffin oil solvents with different volumes, and are uniformly mixed to prepare the attractants with the volume concentrations of 1%, 0.5%, 0.2% and 0.1%, and meanwhile, a yellow armyworm plate and paraffin oil are used as a control group. The number of thrips tabaci on each armyworm plate was recorded 24 hours and 48 hours after suspending the yellow armyworm plate + different concentrations of attractant, and the average was calculated as in table 5.
TABLE 5 outdoor inducing and killing experiment results of different concentrations of attractant for tea yellow thrips
According to theory, as the volume concentration of the attractant is from 0.1%, 0.2%, 0.5% and 1%, the insect-attracting capacity of the attractant tends to increase, but the experimental results in table 5 show that when the volume concentration of the attractant is 0.2%, the insect-attracting effect is remarkable, and the increase of the concentration of the active ingredients has small influence on the insect-attracting capacity of the thrips.
In conclusion, the thrips attractant provided by the invention is prepared by taking benzyl alcohol and phenethyl alcohol as main active ingredients and paraffin oil as a solvent, is matched with a pest sticking plate for use, has high pest trapping rate and excellent effect, and particularly has the best effect of trapping and killing thrips by the attractant with the volume concentration of the benzyl alcohol to the phenethyl alcohol being 2.2:7.8 and the volume concentration of the active ingredients being 0.2 percent, is lasting in time, can obviously improve the trapping capability on the thrips, and has synergistic effect; in addition, the benzyl alcohol and the phenethyl alcohol have low preparation cost and wide sources and are easy to be popularized in a commercialized way.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (4)
1. The thrips attractant is characterized by comprising benzyl alcohol, phenethyl alcohol and paraffin oil, wherein the volume ratio of the paraffin oil is 99.8%, the volume ratio of the benzyl alcohol to the phenethyl alcohol is 2.2:7.8, and the thrips is thrips camellia sinensis.
2. A thrips attractant as claimed in claim 1 for monitoring and controlling thrips.
3. The thrips attractant is used for monitoring, preventing and controlling thrips as claimed in claim 1, and is characterized in that the thrips attractant is loaded into a centrifugal tube carrier to obtain an attraction core; lure the core to hug closely the mythimna separata board upper corner and hang together, pass centrifuging tube lid detain the hole and hang with the mythimna separata board hanging hole, lure the thrips, the centrifuging tube carrier is 1.5ml and covers the centrifuging tube even, opens a plurality of 0.3 mm's hole above the centrifuging tube lid.
4. Application of mango flower extract with benzyl alcohol and phenethyl alcohol as main active ingredients in preparation of a tea thistle horse attractant is disclosed, wherein the volume ratio of the benzyl alcohol to the phenethyl alcohol is 2.2: 7.8.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000239107A (en) * | 1999-02-19 | 2000-09-05 | Sekisui Chem Co Ltd | Low-smelling acaricide |
| CN1593131A (en) * | 2004-07-08 | 2005-03-16 | 河南农业大学 | Noctuid adult attractant |
| CN102217577A (en) * | 2011-04-22 | 2011-10-19 | 北京依科曼生物技术有限公司 | Thrip pest trapping-killing device |
| CN107279136A (en) * | 2016-07-14 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of araecerus fasciculatus attractant and its application |
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2018
- 2018-08-06 CN CN201810885544.9A patent/CN109042648B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000239107A (en) * | 1999-02-19 | 2000-09-05 | Sekisui Chem Co Ltd | Low-smelling acaricide |
| CN1593131A (en) * | 2004-07-08 | 2005-03-16 | 河南农业大学 | Noctuid adult attractant |
| CN102217577A (en) * | 2011-04-22 | 2011-10-19 | 北京依科曼生物技术有限公司 | Thrip pest trapping-killing device |
| CN107279136A (en) * | 2016-07-14 | 2017-10-24 | 中国农业科学院植物保护研究所 | A kind of araecerus fasciculatus attractant and its application |
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