CN109730065B - Attractant composition for frankliniella occidentalis - Google Patents
Attractant composition for frankliniella occidentalis Download PDFInfo
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- CN109730065B CN109730065B CN201810855098.7A CN201810855098A CN109730065B CN 109730065 B CN109730065 B CN 109730065B CN 201810855098 A CN201810855098 A CN 201810855098A CN 109730065 B CN109730065 B CN 109730065B
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Abstract
The invention discloses a Frankliniella occidentalis attractant composition, belonging to the technical field of preparation of insect aggregation pheromones. The Frankliniella occidentalis attractant composition comprises Frankliniella occidentalis gathering pheromone and a synergist, wherein the weight ratio of the Frankliniella occidentalis gathering pheromone to the synergist is 1: 0.5-10, the Frankliniella occidentalis gathering pheromone comprises (R) -lavender acetate and acenaphthylene (S) -2-methylbutyrate as main components, the ratio is 1: 8, and the synergist is selected from plant volatile gathering substances: one or more of alpha-pinene, benzaldehyde and trans-2-hexenal. The Frankliniella occidentalis attractant composition provided by the invention can be accurately applied to the forecast of the condition of Frankliniella occidentalis, the quarantine of pests and the mass trapping, and has important significance for the comprehensive treatment of Frankliniella occidentalis.
Description
Technical Field
The invention belongs to the technical field of preparation of insect aggregation pheromones, and particularly relates to an attractant composition for frankliniella occidentalis.
Background
Frankliniella occidentalis (Frankliniella occidentalis), which belongs to the thrips genus (Frankliniella) of the thrips family (Thripidae) of the order Thysanoptera (Thysanoptera), is one of the world pests with the most serious harm to crops in the world today. Frankliniella occidentalis is a polyphagic pest, and there are about 66 host plants of more than 500 families, including many important commercial crops, such as strawberry, tomato, apple, grape, green pepper, melon, cucumber, chrysanthemum, rose, impatiens balsamina, petunia, datula, dahlia, etc. The pest has rapid propagation speed and wide host range, and can cause rusty spots on leaves and fruits, loss of fruit commodity, and spread Tomato Spotted Wilt Virus (TSWV), Impatiens Necrotic Spot Virus (INSV) and tobacco stripe virus. At present, the pest control agent is widely distributed in more than 60 countries and regions such as Asia, Europe, America, oceania, Africa and the like, wherein the countries such as America, British, Italy, Spanish and the like are widely distributed, are main pests of famous horticultural crops in the world, and are also distributed in Beijing, Shandong, Yunnan and other provinces of China.
The frankliniella occidentalis has strong adaptability, wide host spectrum, strong reproductive capacity and easy diffusion, and is widely applied to the common greenhouse in China. As the trades of flowers and agricultural products become more frequent, the frankliniella occidentalis is likely to spread widely, and the spreading is not considered to be enough to be paid attention before the spreading rather than to be difficult to prevent and treat after the spreading, and a series of measures are taken to avoid the spreading.
At present, the methods for preventing and treating the pests mainly comprise chemical prevention and treatment, physical prevention and treatment and biological prevention and treatment. The long-term use of chemical agents can cause pests to generate drug resistance, kill natural enemies, pollute the environment, destroy the ecological system and have unstable control effect; according to the phototaxis of the frankliniella occidentalis, pest sticking plates with different colors are used for trapping, the pest sticking plates are greatly influenced by natural conditions and can trap non-target insects such as beneficial insects and the like; at present, fungi, bacteria, pathogenic nematodes or predatory natural enemies are used for biological control, and the control effect is not ideal. In view of such a situation, it is urgent to find a pollution-free and sustainable method for controlling thrips. The utilization of insect pheromone is one of effective measures for preventing and treating thrips. The aggregation pheromone is one of insect pheromones, has no residue and pollution due to extremely low dosage, does not generate drug resistance, and is favorable for sustainable treatment of agricultural pests. Referring to patent CN201410604939.9, the best formula of aggregation pheromone of Frankliniella occidentalis is (R) -lavender acetate and carnyl (S) -2-methylbutyrate, with a ratio of 1: 8. According to the principle that insects recognize host plant volatile matters and aggregation pheromone released by male insects has strong trapping capacity on males and females, the formula proportion between the aggregation pheromone and the plant volatile matters of the frankliniella occidentalis can be accurately applied to insect condition prediction, pest quarantine and mass trapping, so that the ideal effect of comprehensively treating the frankliniella occidentalis is achieved.
Disclosure of Invention
The invention aims to provide a Frankliniella occidentalis attractant composition.
The invention provides a Frankliniella occidentalis attractant composition, which comprises Frankliniella occidentalis gathering pheromone and a synergist, wherein the weight ratio of the Frankliniella occidentalis gathering pheromone to the synergist is 1: 0.5-100, the major components of the Frankliniella occidentalis gathering pheromone are (R) -lavender acetate and carnyl (S) -2-methylbutyrate, the ratio is 1: 8, the plant volatile synergist is a plant volatile substance with activity on Frankliniella occidentalis, and the synergist at least comprises one of alpha-pinene, benzaldehyde and trans-2-hexenal.
Preferably, the weight ratio of the frankliniella occidentalis gathering pheromone to the synergist is 1: 1-10.
Optionally, the synergist consists of the following components in parts by weight: 0-9 parts of alpha-pinene, 0-9 parts of benzaldehyde and 0-9 parts of trans-2-hexenal.
Preferably, the synergist comprises the following components in parts by weight: 0-5 parts of alpha-pinene, 5-9 parts of benzaldehyde and 0-5 parts of trans-2-hexenal.
The invention also provides a method for preventing and/or monitoring frankliniella occidentalis, which comprises the step of applying the frankliniella occidentalis attractant composition to a habitat of the frankliniella occidentalis.
Optionally, the Frankliniella occidentalis attractant composition is used in an amount of 0.1-10 mg/m.
Preferably, the amount of the frankliniella occidentalis attractant composition is 0.5-2.5 mg/m.
The invention has the beneficial effects that: 1) the application mode is flexible, the Frankliniella occidentalis attractant composition provided by the invention comprises Frankliniella occidentalis gathering pheromone and a synergist thereof, can be prepared into a synergist attractant core to be matched with the Frankliniella occidentalis gathering pheromone attractant core for use, and can also be prepared into an attractant composition attractant core for use by mixing Frankliniella occidentalis gathering pheromone solution with the synergist solution; various components can be freely selected and used independently or used together according to the proportion, so that the autonomy is stronger; 2) compared with the lure containing only the frankliniella occidentalis aggregation pheromone but no synergist thereof, the lure containing the frankliniella occidentalis aggregation pheromone and the synergist thereof shows stronger trapping activity in field frankliniella occidentalis trapping experiments; 3) the synergist provided by the invention has low component price, can reduce the cost for manufacturing the lure and is suitable for popularization and promotion.
Drawings
FIG. 1 is a schematic diagram of the synthetic route of (R) -lavender acetate.
FIG. 2 is a schematic diagram of the synthesis of carnyl (S) -methylbutyrate.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
The frankliniella occidentalis aggregation pheromone components (R) -lavender acetate, carnyl (S) -methylbutyrate, and the plant volatile component trans-2-hexenal used in the examples below were synthesized by laboratory synthesis, alpha-pinene was purchased from beijing coupled technologies ltd, and benzaldehyde was purchased from feijie healthcare chemical ltd.
Example 1 preparation of (R) -Lavender acetate
2.00g (12.97mmol) of 5-methyl-2- (1-methylethenyl) -4-hexen-1-ol is dissolved in 27mL of anhydrous dichloromethane, 1.97g (19.46mmol) of triethylamine is added as an acid-binding agent, the temperature is kept at about 0 ℃ under the condition of ice salt bath, 1.22g (15.56mmol) of acetyl chloride in dichloromethane is slowly dropped, after 30min of reaction, the temperature is slowly raised to room temperature for continuing the reaction, and TLC is used for monitoring that the raw material is not reduced any more. The reaction was quenched with deionized water, the organic phase was separated, washed with saturated brine (10 mL. times.3) to remove water-soluble impurities, dried over anhydrous magnesium sulfate for 1 hour, filtered under vacuum, and then rotary evaporated under reduced pressure to remove methylene chloride from the filtrate, and the column was separated to give 2.13g (12.97mmol) of a yellow oil with a yield of 83.5%.
EXAMPLE 2 preparation of carnivyl (S) -methylbutyrate
2.00g (12.97mmol) of (Z) -3, 7-dimethyl-2, 6-octadien-1-ol is dissolved in 27mL of anhydrous dichloromethane, 3.94g (38.91mmol) of triethylamine is added as an acid-binding agent, 4-dimethylpyridine is used as a catalyst, the temperature is kept at about 0 ℃ under the condition of ice salt bath, 3.62g (19.46mmol) of (S) - (+) -2-methylbutyric anhydride in dichloromethane is slowly dropped, after 30min of reaction, the temperature is slowly raised to room temperature for continuous reaction, and TLC is used for monitoring until the raw materials are not reduced any more. The reaction was quenched with deionized water, the organic phase was separated, washed with saturated brine (10 mL. times.3) to remove water-soluble impurities, dried over anhydrous magnesium sulfate for 1 hour, filtered under vacuum, and then rotary evaporated under reduced pressure to remove methylene chloride from the filtrate, and the column was separated to give 2.64g (12.97mmol) of a yellow oil with a yield of 85.44%.
Example 3Y-type olfactometer determination of one-component synergists
Preparing a plant-derived volatile single agent: each compound was prepared at 3 concentrations of 0.1, 1, 10. mu.g/. mu.l using n-hexane as a solvent, as a control.
The sensitivity of frankliniella occidentalis to various reagents is measured by a Y-type olfactometer. In a room with air cleanness and a temperature of 25 deg.C and a relative humidity of about 70%, a 1cm x 10cm Y-type olfactometer was used for bioassay. Mu.l of the single-component compound of the synergist was dropped onto a filter paper strip, placed in one side arm of a Y-type olfactometer, and the other side arm was placed with an equal amount of n-hexane as a control. After each group is repeated, the positions of the test group and the control group are exchanged, so that the test error is avoided. The filter paper strip is replaced with a new one after each test, each reagent is repeated for 3 times, and a clean Y-shaped tube needs to be replaced when each reagent is replaced. 1 adult is connected to a position 3cm away from a main arm of the Y-shaped olfactometer, and if the adult stays in any side arm for more than 5 seconds, the adult is considered to have made a selection; if the main arm stays for 3min, no reaction is considered. Each treatment of 30 adults, each treatment of 10 adults with a new Y-tube, and simultaneously 180 degrees of instrument rotation, eliminates the position effect. And finally, observing the distribution condition of the test insects, and calculating the reaction rate, the relative reaction rate and the reaction coefficient to perform data analysis.
As can be seen from Table 1, the reaction rates of the selected test insects are all over 80%, and no significant difference exists among treatments, which indicates that the selected test insects are healthy, and the experimental results of the research are reliable. As can be seen from the selective reaction rate, the reaction values of frankliniella occidentalis for different concentrations of benzaldehyde and trans-2-hexenal have no significant difference, and the reaction values for different concentrations of alpha-pinene have significant difference. The selective reaction rate of frankliniella occidentalis to benzaldehyde is higher, and is obviously higher than that of other synergists with different concentrations. The single-component synergist has attractive activity to the imagoes of the Frankliniella occidentalis, wherein the benzaldehyde has better attractive activity to the Frankliniella occidentalis, and is trans-2-hexenal and alpha-pinene.
TABLE 1 olfactory reaction of Frankliniella occidentalis to a one-component synergist
The data in the table are mean ± sem. Different letters after the same column of data indicate that the difference is significant at a level of P < 0.01 by Duncan's new complex range method.
Example 4 synergistic Effect of different formulation synergists on Silybum occidentalis aggregation pheromone
Alpha-pinene, benzaldehyde, trans-2-hexenal and frankliniella occidentalis gathering pheromone are respectively prepared into n-hexane solution with the concentration of 50 mu g/mu L. Wherein the Frankliniella occidentalis gathering pheromone is (R) -lavender acetate and fleshy (S) -2-methylbutyrate, and is prepared at a ratio of 1: 8.
And (3) sucking a certain amount of each synergist component solution and the Frankliniella occidentalis aggregation pheromone solution by using a liquid transfer gun, and uniformly mixing to obtain the Frankliniella occidentalis plant source aggregation pheromone mixture.
And (3) sucking 200 mu L of Frankliniella occidentalis attractant composition or Frankliniella occidentalis gathering pheromone by using a liquid-transferring gun, dripping 1 ml of dichloromethane in several times after n-hexane is volatilized, and obtaining the Frankliniella occidentalis attractant composition induced core or Frankliniella occidentalis gathering pheromone induced core after the dichloromethane is volatilized. Each lure contains 10mg of frankliniella occidentalis attractant composition or frankliniella occidentalis gathering pheromone. The prepared lure core is put into an aluminum foil for sealing and is stored at the temperature of minus 20 ℃.
When the trapping test is carried out in the field, the frankliniella occidentalis attractant composition attractant core or the frankliniella occidentalis gathering pheromone attractant core is fixed on the armyworm plate. CK1 is blank control without inducing core of Frankliniella occidentalis attractant composition or inducing core of Frankliniella occidentalis gathering pheromone; CK2 control of aggregation pheromone decoys only from frankliniella occidentalis; groups X1 to X3 are treatments of single component synergist wicks only; groups 01 to 03 are treatments of single-component frankliniella occidentalis attractant composition lure; the 04 group to the 06 group are the treatments of the inducing core of the frankliniella occidentalis attractant composition containing the double-component synergist; groups 07 to 09 are treatments of the inducing core of the frankliniella occidentalis attractant composition containing the three-component synergist. Three treatments are arranged for each treatment, the treatments are arranged randomly, and the interval between two adjacent pest sticking plates is 15-30 m. The number of trapped frankliniella occidentalis was recorded and cleared in time every morning from the next day after setting, and the investigation was continued for 30 days.
The test results are shown in table 2. As can be seen from Table 2, the single-component synergist has an attractant activity for Frankliniella occidentalis, but the trapping effect is significantly lower than that of aggregate pheromone of Frankliniella occidentalis. However, the trapping effect of the single-component synergist and the frankliniella occidentalis aggregation pheromone after being mixed is obviously higher than that of the single-component synergist. The synergist and the aggregation pheromone are mixed according to different formula proportions, the trapping effect of the aggregation pheromone induced core is higher than that of the aggregation pheromone, and the dosage of the aggregation pheromone is greatly reduced.
TABLE 2 field synergism of different component synergists on Silybum occidentalis aggregation pheromone
The Frankliniella occidentalis attractant composition provided by the invention can be accurately applied to the forecast of the condition of Frankliniella occidentalis, the quarantine of pests and the mass trapping, and has important significance for the comprehensive treatment of Frankliniella occidentalis. The Frankliniella occidentalis attractant composition is simple to prepare, simple to use, feasible in conditions, strong in trapping capability and good in stability; meanwhile, the Frankliniella occidentalis attractant composition has strong specificity, does not generate resistance, has no harm to natural enemies, is nontoxic to human and livestock, and is environment-friendly; the stress resistance is strong, and the wind, rain and sunshine resistance is realized; the duration is long, and the control effect can be maintained for more than 30 days.
Those of ordinary skill in the art will understand that: the invention is not to be considered as limited to the specific embodiments thereof, but is to be understood as being modified in all respects, all changes and equivalents that come within the spirit and scope of the invention.
Claims (3)
1. The Frankliniella occidentalis attractant composition is characterized by comprising 50 mu L of Frankliniella occidentalis aggregation pheromone and a synergist, wherein the aggregation pheromone is (R) -lavender acetate and carnyl (S) -2-methylbutyrate, the ratio is 1: 8, and the synergist is 10 mu L of alpha-pinene, 120 mu L of benzaldehyde and 20 mu L of trans-2-hexenal.
2. A method for controlling and/or monitoring frankliniella occidentalis, characterized in that a frankliniella occidentalis attractant composition according to claim 1 is applied to the habitat of frankliniella occidentalis.
3. The method for controlling and/or monitoring frankliniella occidentalis as claimed in claim 2, wherein the amount of the frankliniella occidentalis attractant composition is 0.1-10 mg/m.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381255A (en) * | 2014-11-02 | 2015-03-04 | 浙江省农业科学院 | Reagent used for inducing frankliniella occidentalis |
| CN104381256A (en) * | 2014-11-02 | 2015-03-04 | 浙江省农业科学院 | Reagent for attracting frankliniella intonsa |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381255A (en) * | 2014-11-02 | 2015-03-04 | 浙江省农业科学院 | Reagent used for inducing frankliniella occidentalis |
| CN104381256A (en) * | 2014-11-02 | 2015-03-04 | 浙江省农业科学院 | Reagent for attracting frankliniella intonsa |
Non-Patent Citations (2)
| Title |
|---|
| ASSESSING THE ATTRACTIVENESS OF VOLATILE PLANT COMPOUNDS TO WESTERN FLOWER THRIPS Frankliniella occidentalis;ELISABETH H.KOSCHIER et al;《Journal of Chemical Ecology》;20001231;第26卷(第12期);第2643-2655页 * |
| 西花蓟马对不同处理菜豆及其挥发物的行为反应;张骏 等;《生态学杂志》;20151231;第34卷(第2期);第425=430页 * |
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