CN108782559A - A kind of new application for luring Frankliniella occidentalis reagent - Google Patents
A kind of new application for luring Frankliniella occidentalis reagent Download PDFInfo
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Abstract
The present invention provide it is a kind of as the Enhanced agents ingredient for luring Frankliniella occidentalis and non-active ingredient (R)-lavender acetic acid esters prepare lure Frankliniella occidentalis reagent in purposes, wherein the reagent further include as lure reagent Huo Cheng Fen Ni meat base (S) -2-Methyl Butyric Acid Zhi Huo Ni meat base (SThe analog of) -2-Methyl Butyric Acid ester.(R)-lavender acetic acid esters, He Ni meat base (S) -2-Methyl Butyric Acid Zhi Huo Ni meat base (SThe analog of) -2-Methyl Butyric Acid ester, (R)-lavender acetic acid esters Ke Xian write the attracting ability of Ti Gao Ni meat bases (S) -2-Methyl Butyric Acid ester, have very strong synergistic effect.
Description
The application is Chinese invention patent application, application number 2014106049399, the applying date：On November 2nd, 2014
Divisional application.
Technical field
The invention belongs to plant protection art, more particularly to a kind of Frankliniella occidentalis containing aggregation pheromone component is lured
Substance and its field use in conjunction technology with colour table.
Background technology
The small insects such as thrips, aleyrodid, red spider, liriomyza bryoniae and aphid every year to the Important Economic crop in China, including
Vegetables, flowers etc. cause serious harm.If Frankliniella occidentalis (Frankliniella occidentalis) is with rasping-sucking mouthparts
Piercing and sucking host plant juice, tender leaf of causing harm, blade, floral organ, delay and are bloomed at lamina, blade and spend lopsided atrophy and discoloration, or
Fallen flowers, shedding, young fruit of causing harm cause scar and deformity.It also propagates serious tomato spotted wilf virus (TSWV) of causing harm simultaneously, by
Evil product yield is remarkably decreased or quality variation cannot be sold, and plant cannot grow completely when serious.
Currently, the control method about thrips mainly has 3 kinds：Chemical prevention, biological control and physical control (Wu Qingjun
Deng 2005；Zhong Feng etc., 2009).But since thrips has development duration shorter, individual little Yi is hidden, and energy is strong, and right
Most of insecticides tested is also easy to produce the features such as resistance, and leading to chemical prevention and physical control, the effect is unsatisfactory.In view of such
It is extremely urgent to find a kind of nuisanceless sustainable thrips control method for present situation.And it is prevention thrips using insect pheromone
One of effective measures (Hamilton et al., 2005).Insect pheromone arises directly from insect body, as a kind of non-
Chemical Control measure can be not only used for pest population dynamics monitoring, while can also be combined with other prevention and control measures to improve evil
Worm control effect.Aggregation pheromone is one kind of insect pheromone, is had studies have shown that being prevention Ji using aggregation pheromone
One of effective measures of horse (Kirl and Hamilton, 2004；Hamilton et al., 2005), due to the use of dosage pole
It is low, and worked in the form of discharging in air, noresidue problem will not bring harm to human health and environment, and anti-
Property pest identification or attracting ability will not reduce, therefore be conducive to the improvement of resistant insect, at the same also can effectively protect from
Natural enemy in right boundary, is conducive to the integrated application of agricultural pests.
The current correlative study in relation to thrips aggregation pheromone Components identification and bioactivity and Field information is domestic and international
Still less.So far, only the aggregation pheromone of Frankliniella occidentalis and flower thrips (Frankliniella intonsa) at
Get and efficiently separate and identify, the aggregation pheromone main active component of two kinds of thrips is (R)-lavender acetic acid esters
(R)-lavandulyl acetate) are He Ni meat base (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate)
(Hamilton et al.,2005；Lv Yaobin, wishes dawn cloud etc., 2012).Although the aggregation pheromone component of Frankliniella occidentalis obtains
The separation such as Britain expert Hamilton identify that still, these experts are also considered as and Ni meat base (S) -2-Methyl Butyric Acid ester simultaneously
(neryl (S) -2-methylbutanoate) some related compounds of molecular structure of compounds, such as isobornyl
valerate、isobornyl 2-methyl butanoate、 isobornyl pivalate、lavandulyl valerate
With as aggregation pheromone Zu Fen Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) all to west
Flower thrips has strong attractive activity.These experts have used in test the release device of plastic colorimetric plates+lure, the device
Colour table can be used yellow, white and blue, and lure in dipped Rubber end or glue with attractive activity compound using dissolving in
The mode of reactive compound, and provide a field case study on implementation, and applied related patents (United States Patent (USP), No.US 7,
985,412 B2, Jul.26,2011).But Hamilton etc. then thinks, the component (R)-found in Frankliniella occidentalis smokes clothing
Oxalacetate (R)-lavandulyl acetate) there is no bioactivity, there cannot be individual attracting action to west flower Ji.
In addition, above-mentioned Britain expert thinks (R)-lavender acetic acid esters ((R)-lavandulyl acetate) in its paper
Yu Ni meat base (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) do not show increasing in field trials
Effect acts on, and the evidence provided is：(1) (R)-lavender acetic acid esters ((R)-lavandulyl acetate) Yu Ni meat bases
(S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) 1:1 ratio using capture Frankliniella occidentalis quantity with
The quantity that capture is used alone in Ni meat base (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) is essentially identical
, without difference；(2) his indoor measurement (R)-lavender acetic acid esters ((R)-lavandulyl acetate) Yu Ni meat bases
(S) proportional region of both -2-Methyl Butyric Acid esters (neryl (S) -2-methylbutanoate) is 1:0.8-1:5, he thinks
There are an amplitude ranges for ratio both in the case of nature, and there is no this best situations of some special ratios；(3) he thinks
(R) effect of-lavender acetic acid esters ((R)-lavandulyl acetate) is unknown.
In short, existing technology shows (R)-lavender acetic acid esters ((R)-lavandulyl acetate) Dui Ni meat bases
(S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) does not have synergistic effect, without being used in mixed way.
Invention content
But we study surprisingly it has been found that, 2 components in Frankliniella occidentalis aggregation pheromone play an important role,
Zhong Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) individually have attracting ability, although
(R)-lavender acetic acid esters ((R)-lavandulyl acetate) (Chinese name：(R)-lavender acetic acid esters) do not have individually
Attracting ability, but when (R)-lavender acetic acid esters ((R)-lavandulyl acetate)) Yu Ni meat base (S) -2- methyl fourths
When acid esters (neryl (S) -2-methylbutanoate) coexists, (R)-lavender acetic acid esters ((R)-lavandulyl
What acetate) Ke Xian write Ti Gao Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) lures energy
Power has very strong synergistic effect.
Mixed not only dramatically increase of this compound lures worm amount, in addition, the ratio of the two also seems of crucial importance, when him
Ratio in Frankliniella occidentalis geographical population body both component ratios and when content, attracting ability is higher.Pass through
Our experiment, it is believed that there are optimal proportions, i.e., (R)-lavender acetic acid esters ((R)-lavandulyl acetate) with
Ni meat base (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) ratio is 1:8, but active section is 1:
0.5-1:Between 12, and there is also the concentration ranges that suitable field uses for the concentration of two compounds, I tests Ren Wei Shi Ni meat
Base (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) exclusive use needs 1000ng to 100000ng,
That is 1 μ g-100 μ g；(R)-lavender acetic acid esters ((R)-lavandulyl acetate) Yu Ni meat bases (S) -2-Methyl Butyric Acid ester
Referring also to above-mentioned concentration ranges when (neryl (S) -2-methylbutanoate) is used in mixed way.
Group of the present invention not only confirms the key component of aggregation pheromone in the Frankliniella occidentalis population for be distributed in Chinese field
For (R)-lavender acetic acid esters ((R)-lavandulyl acetate) Yu Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -
2-methylbutanoate), it and by aggregation to Frankliniella occidentalis, fights and the observation of mating behavior, and indoor and field
Between test clear Ni meat base (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) China field make
Concentration ranges；Research also indicates that domestic Frankliniella occidentalis aggregation pheromone component ratio and external report are different
, this research, which also provides, is suitble to the best composition ratio of China's trapping Frankliniella occidentalis and field to use concentration range, and gives in detail
The operating method how field traps and kills Frankliniella occidentalis together with colour table is gone out.
Therefore, one aspect of the present invention provides a kind of reagent for luring Frankliniella occidentalis, which includes (R)-lavender
((R)-lavandulyl acetate) are He , Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -2- for acetic acid esters
) or its analog methylbutanoate.
You selects , Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) to be used as attractant
The active ingredient of formula, (R)-lavender acetic acid esters ((R)-lavandulyl acetate) Zuo Wei Ni meat base (S) -2- methyl
The building component of butyrate (neryl (S) -2-methylbutanoate) component.
Preferably, (R)-lavender acetic acid esters ((R)-lavandulyl acetate) Yu Ni meat base (S) -2- methyl fourths
The mass ratio of acid esters (neryl (S) -2-methylbutanoate) two kinds of components is 1:0.5,1:1,1:2,1:4, 1:8,1:
10 or 1:12.Preferably, (R)-lavender acetic acid esters ((R)-lavandulyl acetate) Yu Ni meat base (S) -2- methyl fourths
The mass ratio of acid esters (neryl (S) -2-methylbutanoate) two kinds of components is 1:0.5---1:12.
You selects , Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) actual concentration
For 0-100000ng.Preferably, a concentration of 0- of (R)-lavender acetic acid esters ((R)-lavandulyl acetate)
100000ng。
Preferably, the Frankliniella occidentalis is located at crops, on garden crop.It is furthermore preferred that the Frankliniella occidentalis is
Cucumber, capsicum, pimento, tomato, strawberry, grape, orchid, the Frankliniella occidentalis on various chrysanthemums.
You selects the analog of , Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) to be
The compound of following logical formula (I),
Wherein, (1) R1Can be monoterpenyl groups (monoterpene alkenyl), R2It is C3-C5Group；Alternatively, (2) R1It can
To be isobornyl groups (isobornyl)；Alternatively, (3) R1Can be lavandulyl groups (lavender base)；Alternatively,
(4)R1Can be neryl group (Ni meat base)；Or, 5) R2Can be the alkyl (alkyl) or alkylene of linear chain or branched chain
(alkylene)；Or, (6) R2Can be C4Group；Or, (7) R2- C (O) O- groups can be a valerate group (penta
Acidic group), isovalerate groups (isoamyl acidic group), pivalate (trimethylace tonitric base) or 2-methyl butanoate bases
Group's (2-Methyl Butyric Acid base)；Or (8) R2- C (O) O- groups can be (S) -2-methylbutanoate group
((S) -2- methylbutanoic acids base)；(9) formula 1 can be made of one or more following groups：Isobornyl valerate (penta
Sour isobornyl thiocyanoacetate), isobornyl isovalerate (Isobornyl isovalerate), isobornyl pivalate (trimethyls
Isobornyl acetate), isobornyl 2-methyl butanoate (2-Methyl Butyric Acid isobornyl thiocyanoacetate), lavandulyl
Valerate (lavender valerate), lavandulyl isovalerate (lavender isovalerate), lavandulyl
Pivalate (trimethylace tonitric lavender ester), and lavandulyl 2-methyl butanoate (lavender 2- methyl fourths
Acid esters).
Description of the drawings
Fig. 1 is attracting ability (Control=0ng/ μs of (the R)-lavandulys acetate to Frankliniella occidentalis female adult pest
L) experimental result picture.
Fig. 2 is attracting ability experimental result pictures of (the R)-lavandulys acetate to Frankliniella occidentalis male imago.
Fig. 3 is attracting ability experimental result pictures of (the R)-lavandulys acetate to the female male imago of Frankliniella occidentalis.
Fig. 4 is attracting ability experimental result pictures of neryl (the S) -2-methylbutanoate to Frankliniella occidentalis female adult pest.
Fig. 5 is attracting ability experimental result pictures of neryl (the S) -2-methylbutanoate to Frankliniella occidentalis male imago.
Fig. 6 is attracting ability experimental results of neryl (the S) -2-methylbutanoate to the female male imago of Frankliniella occidentalis
Figure.
Fig. 7 different proportions (R)-lavandulys acetate and neryl (S) -2-methylbutanoate to male and female at
The attracting ability experimental result picture of worm.
Fig. 8 different proportions (R)-lavandulys acetate and neryl (S) -2-methylbutanoate to male and female at
The synergistic effect experimental result picture of worm attracting ability.
Advantageous effect
The advantages of this product is：Frankliniella occidentalis is a kind of invasive pest, and the product of the present invention and materialization is to flower thrips category
This pest of Frankliniella occidentalis has excellent attractant effect, i.e. high sensitivity, reagent can be lured as height, to kill flower
Thrips.The present invention and materialization product individually or with insecticide combination force down insect population also as the attractant of Frankliniella occidentalis,
Pest population quantity is controlled, to achieve the purpose that protect production volume increase.
It is described in detail
Compound
(R) molecular structural formula (abbreviation R compounds) of-lavender acetic acid esters ((R)-lavandulyl acetate) is such as
Under：
Molecule (the abbreviation S chemical combination of Ni meat base (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate)
Object) structural formula is as follows：
It is similar Yu Ni meat base (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) molecular structure, tool
Active compound or derivative are as follows：
Wherein (1) R1Can be monoterpenyl groups, R2It is C3-C5 groups；
(2)R1Can be isobornyl groups；(3)R1Can be lavandulyl groups；(4)R1Can be neryl bases
Group；(5)R2Can be alkyl the or alkylene groups of linear chain or branched chain；(6)R2Can be C4Group；(7) R in formula 12-
C (O) O- groups can be valerate, isovalerate, pivalate or 2-methyl butanoate group；
(8) R in formula 12- C (O) O- groups can be (S) -2-methylbutanoate group；(9) formula 1 can be by one
Or multiple following groups compositions：Isobornyl valerate (isobornyl valerate), isobornyl isovalerate are (different
Isobornyl valerate), isobornyl pivalate (trimethylace tonitric isobornyl thiocyanoacetate), isobornyl 2-methyl
Butanoate (2-Methyl Butyric Acid isobornyl thiocyanoacetate), lavandulyl valerate (lavender valerate), lavandulyl
Isovalerate (lavender isovalerate), lavandulyl pivalate (trimethylace tonitric lavender ester), and
Lavandulyl 2-methyl butanoate (lavender 2-Methyl Butyric Acid ester).
S compounds and derivative (, that is, Yu Ni meat bases (S) -2-Methyl Butyric Acid ester
(neryl (S) -2-methylbutanoate) some related compounds of molecular structure of compounds, such as
Isobornyl valerate (isobornyl valerate), isobornyl 2-methyl butanoate (the different ice of 2-Methyl Butyric Acid
Piece ester), isobornyl pivalate (trimethylace tonitric isobornyl thiocyanoacetate), lavandulyl valerate (lavender isoamyls
Acid esters).
The mixture ratio of R and S
(R)-lavender acetic acid esters ((R)-lavandulyl acetate) Yu Ni meat bases (S) -2-Methyl Butyric Acid ester
(neryl (S) -2-methylbutanoate) ratio can be 1:8, but active section is 1:0.5-1:Between 12, and two
There is also the concentration ranges that suitable field uses for the concentration of a compound.Ni meat base (S) -2-Methyl Butyric Acid ester (neryl (S) -
It 2-methylbutanoate) is used alone and needs 1000ng to 100000ng, i.e. 1 μ g-100 μ g；(R)-lavender acetic acid esters
((R)-lavandulyl acetate) Yu Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate)
Referring also to above-mentioned concentration ranges when being used in mixed way.
The preparation of the mixture of R and S
First (R)-lavandulys acetate and neryl (S) -2-methylbutanoate of content >=99% is used
N-hexane (99%, HPLC grade, Scharlau) is diluted to required concentration respectively.Lure carrier is green natural rubber
(1cm diameters × 1.5mm long), each weighs about 0.75g.Then according to a certain percentage by (R)-lavandulys acetate and
Neryl (S) -2-methylbutanoate is injected separately into Rubber end, after about 5 minutes air-dry, is wrapped with masking foil, be placed on -
It is for use in 20 DEG C of refrigerators.
Embodiment
The present embodiment is only to have done limited experiment to some limited experiments of the present invention, these experiments are only
It is to make explanations to the marrow of the present invention and how exemplary expression implements the present invention, but these experiments are not pair
The limitation of invention scope, the scope of the present invention are limited by claim.
Embodiment 1
Group of the present invention is in the component analysis of series of indoor Frankliniella occidentalis, behavior observation, bioassay and artificial synthesized phase
On the working foundation for closing component, specifies domestic population Frankliniella occidentalis aggregation pheromone key component, ratio and content, have
The concentration range and proportional region of attractive activity and artificial synthesized aggregation pheromone quality analysis, and carry out on this basis
Field trial verification.
1. field activity test method
Artificial synthesized aggregation pheromone (R)-lavandulys acetate and neryl (S) -2-methylbutanoate
To the field biological activity test of Frankliniella occidentalis in July, 2014 Hebei province's Zhangjiakou City big shed eggplant carry out.
Testing region, nearby and originally experimental field long-term cropping is eggplant, using Plastic canopy culture mode.Experiment
Period is the eggplant collecting period, is alreadyd exceed before experiment one week and insecticidal bactericide is not used.Frankliniella occidentalis worm is carried out before this experiment
Mouth radix investigation, has selected the relatively large number of greenhouse of wherein 2 worm amounts to carry out experiment, two greenhouse area about 600m2
(100m long × 6m wide).Each greenhouse has 4 plots of land, per the wide 1.5m of furrow, plants 2 row eggplants above, 4 furrow plant 8 row eggplants altogether.
About 15 meters of distance between two greenhouses, centre have 2 kinds of greenhouses for being implanted with other crops to make isolation use, avoid lure smell
It influences each other.Common pratia fruit plant height about 100cm is tested, 2 furrow to keep to the side is selected to arrange thrips trap in 4 plots of land, it should
Trap is made of 1 root long 1.8m (being inserted into 30cm in soil) bamboo poles and the Yellow sticky trap being fixed at the top of bamboo pole.Yellow plate is big
Small is long 25cm × wide 20cm, and the lure containing various concentration aggregation pheromone is fixed on yellow plate by the aperture in colour table center
On.Yellow plate setting direction is vertical with the direction of plant row, and is higher by about 5cm at the top of crop.
Test the making of lure：First by (R)-lavandulys acetate and neryl (S) -2- of high-purity
Methylbutanoate n-hexanes (99%, HPLC grade, Scharlau) be diluted to respectively 10ng/ μ l, 100ng/ μ l,
4 concentration gradients such as 1000ng/ μ l and 10000ng/ μ l, wherein locating in order to control without any potent agent ingredient (0ng/ μ l)
Reason.
Meanwhile the mixture total concentration for simulating Frankliniella occidentalis aggregation pheromone proportioning is (R)-lavandulys acetate
It is respectively set to 1 with neryl (S) -2-methylbutanoate ratios:0.5(1000ng+500ng),1:1 (1000ng+
1000ng),1:2(1000ng+2000ng),1:4 (1000ng+4000ng) and 1:8 (1000ng+8000ng).Lure is tested to carry
Body is green natural rubber (1cm diameters × 1.5mm long), each weighs about 0.75g.
The preparation of lure carrier between different disposal processing：Lure carrier first uses chromatographically pure n-hexane to impregnate before experiment
For 24 hours, then 2h is dried in 50 DEG C of baking ovens.Lure carrier, which immerses, to be filled in the wide mouth glass bottle for diluting synthesis compound, is impregnated
It takes out afterwards for 24 hours, indoor shady place natural air drying (about 30min).Lure is compareed to impregnate using only n-hexane.What is completed lures
Core is wrapped with masking foil, is placed in -20 DEG C of refrigerators for use.
14 processing are respectively provided with 4 repetitions, 2 rows respectively 1 repetition of setting of keeping to the side in each greenhouse, often 14 processing in row
Random distribution.Replace yellow plate and lure within every 2 days during experiment.14 processing be independent two kinds of reagents (10 processing) respectively with
And the processing (4 processing) of their mix reagents.
2 data analyses
Data processing is carried out using SPSS13.0 softwares, and difference is aobvious between the investigation of field trial insect population and different disposal
Work property is examined using the Duncan duncan's new multiple range methods in single factor experiment statistical analysis.
3 results and analysis
3.1 (R)-lavandulys acetate are individually to the attracting ability of Frankliniella occidentalis adult
(the R)-lavandulys acetate that field trial has evaluated one of artificial synthesized aggregation pheromone component are different
Attracting ability of the concentration to the female male imago of Frankliniella occidentalis on eggplant.Experiments have shown that (see Fig. 1-3), single compound (R)-
Lavandulys acetate are female to Frankliniella occidentalis, male imago is without attractive activity, do not have conspicuousness poor between each concentration
It is different.
Process is separately configured in R compounds and lure carrier prepares the method phase described with " making of experiment lure "
Together, 4 concentration gradients such as concentration difference 10ng/ μ l, 100ng/ μ l, 1000ng/ μ l and 10000ng/ μ l of configuration；Wherein control
Concentration (compareing concentration) processed is 0ng/ μ l.
Attracting abilities of 3.2 neryl (the S) -2-methylbutanoate to the female male imago of Frankliniella occidentalis
Neryl (S) -2-methylbutanoate that field trial continues to have evaluated one of aggregation pheromone component is different
Attracting ability of the concentration to the female male imago of Frankliniella occidentalis on eggplant.Experiments have shown that (being shown in Table Fig. 4-6), single compound neryl
(S) -2-methylbutanoate has attractive activity to the female male imago of Frankliniella occidentalis.Wherein when single lure neryl (S)-
2-methylbutanoate contents are in 1000ng or more, compared with the control, lure worm amount to increase 15.2- female adult pest
22.7%.Single lure neryl (S) -2-methylbutanoate contents are in 100,1000 and 10000ng, to male imago
Lure worm amount that can increase by 12.4%, 2.2% and 41.6% respectively.When regardless of male and female, neryl (S) -2-methylbutanoate
Content can increase respectively at 1000 and 10000ng lures worm amount 17.6% and 21.8%.The composite score table of the compound
It is bright, in set concentration range, as concentration increase lures worm amount to increase, wherein when single lure 10000ng to male and female at
The attracting ability of worm is best.
To west flower under 3.3 (R)-lavandulys acetate and neryl (S) -2-methylbutanoate different proportions
The attracting ability of thrips adult
Since the aggregation pheromone discharged in Frankliniella occidentalis body includes various ingredients, wherein highest two kinds of groups of content
It is divided into (R)-lavandulys acetate and neryl (S) -2-methylbutanoate.Although external existing research is thought
It is not no bioactivity that (R)-lavandulys acetate, which are used alone, our interior and field trial (such as Fig. 1-3,
Shown in Fig. 4-6) also confirm that single component individually without display attractive activity.
But our research groups think, from the angle analysis of biological evolution, (R)-lavandulys acetate can
Biological function can be undertaken in other ways.Therefore we are according to (R)-lavandulys in domestic Frankliniella occidentalis population
The proportional region (1 of both acetate and neryl (S) -2-methylbutanoate:3.50-1:11.95), and with reference to outer
The ratio of both state's documents, sets 1:0.5,1:1,1:2,1:4 and 1:8 experiment ratio.Result of study shows (Fig. 7-8),
(R)-lavandulys acetate have extremely significant synergistic effect to neryl (S) -2-methylbutanoate, can show
Write the attractive activity improved to the female male imago of Frankliniella occidentalis.(R)-lavandulys acetate and neryl (S) -2-
Methylbutanoate is 1:0.5,1:1,1:2,1:4,1:When 8 ratio, compared with the control, always lure worm amount (containing it is female, male at
Worm) 44.13%, 45.25%, 77.65%, 75.42% and 141.34% can be increased separately.(see Fig. 7)
Experiment also shows (Fig. 8) simultaneously, (contains neryl (S) -2- with the lure with most strong attracting ability
Methylbutanoate 10000ng) it compares, (R)-lavandulys acetate and neryl (S) -2-
Methylbutanoate is 1:0.5,1:1,1:2,1:4,1:When 8 ratio, female male imago can be increased in general
18.35%, worm amount, effect highly significant are lured 19.27%, 45.87,44.04% and 98.17%.
Embodiment 2
Compared with examples of implementation 1, Chu Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -2-
Methylbutanoate) by its analog (isobornyl valerate, isobornyl 2-methyl butanoate,
Isobornyl pivalate or lavandulyl valerate) replace outside, it is other all identical as examples of implementation, by same
The similar a series of experiments of sample obtains roughly the same result (specific data summary), i.e. (R)-lavandulys acetate
The analog of Dui Ni meat bases (S) -2-Methyl Butyric Acid ester also shows very strong synergistic effect.This further demonstrates (R)-
Lavandulys acetate Dui Ni meat bases (S) -2-Methyl Butyric Acid ester (neryl (S) -2-methylbutanoate) it is similar
Object also has very strong synergistic effect.
Claims (4)
1. as the Enhanced agents ingredient for luring Frankliniella occidentalis non-active ingredient (R)-lavender acetic acid esters is lured in preparation
Purposes in Frankliniella occidentalis reagent, wherein the reagent further include as lure reagent Huo Cheng Fen Ni meat base (S) -2- methyl
Butyrate Huo Ni meat base (SThe analog of) -2-Methyl Butyric Acid ester.
2. purposes , according to claim 1 wherein , Ni meat bases (SThe analog of) -2-Methyl Butyric Acid ester is following general formula
Compound,（General formula 1）Wherein,（1）R1Can be monoterpene alkenyl, R2It is C3-C5Group；Alternatively,（2）R1
It can be isobornyl；Alternatively,（3）R1It can be lavender base；Alternatively,（4）R1Ke Yi Shi Ni meat bases；Or, 5）R2Can be straight
The alkyl or alkylene of chain or branch；Or,（6）R2Can be C4Group；Or,（7）R2- C (O) O- groups can be a valeric acid
Base, isoamyl acidic group, trimethylace tonitric base or 2-Methyl Butyric Acid base；Or（8）R2- C (O) O- groups can be one (S)-2-
Methylbutanoic acid base；Or（9）General formula 1 can be made of one or more following groups：Isobornyl valerate, isovaleric acid isoborneol
Ester, trimethylace tonitric isobornyl thiocyanoacetate, 2-Methyl Butyric Acid isobornyl thiocyanoacetate, lavender valerate, lavender isovalerate, trimethyl second
Sour lavender ester and lavender 2-Methyl Butyric Acid ester；
It is a kind of for luring the reagent of Frankliniella occidentalis, wherein the reagent include (R)-lavender acetic acid esters, He Ni meat base (S)-2-
Methylbutyrate Huo Ni meat base (SThe analog of) -2-Methyl Butyric Acid ester.
Draw 3. purposes , according to claim 1 or 2 wherein , Ni meat base (S) -2-Methyl Butyric Acid esters or its analog are used as
The active ingredient of lure formula, the building component of (R)-lavender acetic acid esters Zuo Wei Ni meat bases (S) -2-Methyl Butyric Acid ester component.
4. purposes according to claim 1, wherein the west flower Ji is cucumber, capsicum, pimento, tomato, strawberry, Portugal
Grape, orchid, the Frankliniella occidentalis on various chrysanthemums.
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| CN201410604939.9A CN104381255B (en) | 2014-11-02 | 2014-11-02 | A kind of reagent for being used to lure Frankliniella occidentalis |
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| CN105557692B (en) * | 2016-03-22 | 2018-02-16 | 福建省农业科学院茶叶研究所 | A kind of thrips attractant based on tea plant volatiles |
| CN108041034B (en) * | 2018-01-03 | 2020-09-08 | 贵州省茶叶研究所 | Attractant for female thrips tea rod gathering pheromone and method for preparing attractant core of attractant |
| CN109730065B (en) * | 2018-07-23 | 2022-04-01 | 黑龙江省植检植保站 | Attractant composition for frankliniella occidentalis |
| CN110857937A (en) * | 2018-08-22 | 2020-03-03 | 浙江省农业科学院 | Method for measuring release rate of thrips palmi aggregated pheromone in carrier |
| CN110857932A (en) * | 2018-08-22 | 2020-03-03 | 浙江省农业科学院 | Method for measuring release rate of thrips aggregation pheromone in rubber plug carrier |
| WO2023144388A1 (en) * | 2022-01-31 | 2023-08-03 | Hassani Mounir | Insect pest control by a mixture of luring substances with pathogenic bio insecticides |
| CN116035003A (en) * | 2023-01-12 | 2023-05-02 | 福建省农业科学院植物保护研究所 | Biodegradable thrips attracting and smearing adhesive and application method thereof |
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| US7985412B2 (en) * | 2001-12-21 | 2011-07-26 | Keele University | Method of monitoring/controlling thysanoptera |
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| GB201219513D0 (en) * | 2012-10-30 | 2012-12-12 | Univ Keele | Method of monitoring and/or controlling thysanoptera |
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Non-Patent Citations (3)
| Title |
|---|
| ANCA DAFINA COVACI等: "Evaluation of Pheromone Lure as Mass-Trapping Tools for Western Flower Thrips", 《BULLETIN UASVM AGRICULTURE》 * |
| IAN A. N. DUBLON: "The aggregation pheromone of the Western flower thrips", 《THESIS (PH.D.) OF KEELE UNIVERSITY》 * |
| JAMES G. C. HAMILTON等: "IDENTIFICATION OF A MALE-PRODUCED AGGREGATION PHEROMONE IN THE WESTERN FLOWER THRIPS Frankliniella occidentalis", 《JOURNAL OF CHEMICAL ECOLOGY》 * |
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