CN110050789A - A kind of novel tarnished plant bug attractant and the preparation method and application thereof - Google Patents

A kind of novel tarnished plant bug attractant and the preparation method and application thereof Download PDF

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Publication number
CN110050789A
CN110050789A CN201910473482.5A CN201910473482A CN110050789A CN 110050789 A CN110050789 A CN 110050789A CN 201910473482 A CN201910473482 A CN 201910473482A CN 110050789 A CN110050789 A CN 110050789A
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tarnished plant
novel
plant bug
nopinene
dodecane
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CN110050789B (en
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王兰
迪丽努尔·艾麦提
冯宏祖
曹玉
刘慧敏
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Tarim University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)

Abstract

The embodiment of the invention discloses a kind of novel tarnished plant bug attractants and the preparation method and application thereof, wherein, the raw material for preparing the novel tarnished plant bug attractant includes nonadecane, nopinene and dodecane, and nonadecane, the volume ratio of nopinene and dodecane is 1:(1-5): (5-1), the nonadecane, nopinene and dodecane are being diluted to 1:1000 using preceding respectively, 1:10000, 1:1000 times of liquid, the novel tarnished plant bug attractant utilizes nonadecane, nopinene and dodecane are main component, with preferable trap effect, adult is trapped and killed by field, it can reach the purpose of pest control.

Description

A kind of novel tarnished plant bug attractant and the preparation method and application thereof
Technical field
The present embodiments relate to agricultural technology fields, and in particular to a kind of novel tarnished plant bug attractant and application.
Background technique
Miridae is 1 section of Insecta Semiptera.Fore wing has key, without simple eye terrestrial stinkbug class insect.It is commonly referred to as fleahopper. For section maximum in Semiptera, nearly ten thousand kinds known to the world, major area is distributed.Miridae insect majority moves on plant, Action is active, quite kind to circle in the air.Most monoids are mainly phytophagous, and host range is extensive, including angiosperm, coniferous tree and fern Class.Outstanding to like the organs of multiplication such as piercing and sucking flower, flower bud, fruit in addition to the blade of sucking plant, numerous species, which have significantly to chase, blooms The habit of plant.Numerous species are in addition to feeding plant food, normal and some other small flexibles of food insects, mating period This habit is particularly evident.Therefore, even if some harmful types also can be beneficial to the mankind because preying on other pests.Part class Group then based on predatism, becomes the natural enemy of the pests such as aphid.
Tarnished plant bug belongs to Lygus Hahn, and life habit is northern 1 year raw 3-4 generation, with adult weeds, dry branches and fallen leaves, It is overwintering under soil rock.It comes out of hibernation after next spring host germination activity, likes the piercing and sucking juice on tender leaf, tender stem, bud, at one section of feeding Between after start mating, oviposition, ovum fecund is in tender stem, petiole, vein or bud, the ovum phase about 10 days.Nymph totally 5 age, through more than 30 days Turn into adult.At the happiness activity on daytime of, nymph, early, late feeding is most contained, and activity rapidly, is good at hidden keep away.The generation phase is irregular, June Cotton field often is moved into, autumn is moved back to again on xylophyta or autumn vegetable.Natural enemy mainly have egg parasitoids, predacious spider, a Ji hunt stinkbug, Flower stinkbug etc..
Currently, during the prevention and treatment of tarnished plant bug, generally catch and kill prevention and treatment using chemical pesticide, but chemical agent A large amount of and unreasonable application causes the serious pollution to farmland ecological environment.
Summary of the invention
For this purpose, the embodiment of the present invention provides a kind of novel tarnished plant bug attractant and application, to solve to adopt in the prior art The problem of farmland ecological environment is seriously polluted with chemical pesticide.
To achieve the goals above, the embodiment of the present invention provides the following technical solutions:
According to a first aspect of the embodiments of the present invention, a kind of novel tarnished plant bug attractant is provided, described novel herd is prepared The raw material of lygus bug attractant includes nonadecane, nopinene and dodecane, and the volume ratio of nonadecane, nopinene and dodecane is 1:(1—5):(5—1)。
Further, the volume ratio of nonadecane, nopinene and dodecane is 1:1:5.
Further, the volume ratio of nonadecane, nopinene and dodecane is 1:5:1.
Further, the volume ratio of nonadecane, nopinene and dodecane is 1:3:3.
Further, the volume ratio of nonadecane, nopinene and dodecane is 1:1:1.
Further, the volume ratio of nonadecane, nopinene and dodecane is 1:5:5.
According to a second aspect of the embodiments of the present invention, a kind of preparation method of novel tarnished plant bug attractant is provided, including Following steps:
Step 1: nonadecane, nopinene and dodecane are diluted to 1:1000,1:10000,1:1000 times of liquid respectively, it is standby With;
Step 2: nonadecane, nopinene and dodecane are pressed 1:(1-5): the volume ratio of (5-1) mixes.
According to a third aspect of the embodiments of the present invention, a kind of novel tarnished plant bug attractant is provided in trapping tarnished plant bug Application.
According to a fourth aspect of the embodiments of the present invention, a kind of attractant is provided, is lured comprising above-mentioned novel tarnished plant bug Agent.
The embodiment of the present invention has the advantages that the embodiment of the present invention provides a kind of novel tarnished plant bug attractant and answers With the novel tarnished plant bug attractant is main component using nonadecane, nopinene and dodecane, has preferable trapping effect Fruit can reduce applications of pesticide number and dosage using tarnished plant bug attractant, reduce ecological pollution, by field trapping at Worm achievees the purpose that pest control.
Detailed description of the invention
It, below will be to embodiment party in order to illustrate more clearly of embodiments of the present invention or technical solution in the prior art Formula or attached drawing needed to be used in the description of the prior art are briefly described.It should be evident that the accompanying drawings in the following description is only It is merely exemplary, it for those of ordinary skill in the art, without creative efforts, can also basis The attached drawing of offer, which is extended, obtains other implementation attached drawings.
Fig. 1 is 10 in present invention test one-1(V/V) field of the plant volatiles of concentration to the female male imago of tarnished plant bug Between trap effect;
Fig. 2 is 10 in present invention test one-2(V/V) field of the plant volatiles of concentration to the female male imago of tarnished plant bug Between trap effect;
Fig. 3 is 10 in present invention test one-3(V/V) field of the plant volatiles of concentration to the female male imago of tarnished plant bug Between trap effect;
Fig. 4 is 10 in present invention test one-4(V/V) field of the plant volatiles of concentration to the female male imago of tarnished plant bug Between trap effect.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation Content disclosed by book is understood other advantages and efficacy of the present invention easily, it is clear that described embodiment is the present invention one Section Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not doing Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.
Embodiment 1
The present embodiment provides a kind of novel tarnished plant bug attractants, prepare the raw material packet of the novel tarnished plant bug attractant Nonadecane, nopinene and dodecane are included, and the volume ratio of nonadecane, nopinene and dodecane is 1:(1-5): (5-1).
Embodiment 2
The present embodiment provides a kind of novel tarnished plant bug attractants, prepare the raw material packet of the novel tarnished plant bug attractant Nonadecane, nopinene and dodecane are included, and the volume ratio of nonadecane, nopinene and dodecane is 1:1:5.
Embodiment 3
The present embodiment provides a kind of novel tarnished plant bug attractants, prepare the raw material packet of the novel tarnished plant bug attractant Nonadecane, nopinene and dodecane are included, and the volume ratio of nonadecane, nopinene and dodecane is 1:5:1.
Embodiment 4
The present embodiment provides a kind of novel tarnished plant bug attractants, prepare the raw material packet of the novel tarnished plant bug attractant Nonadecane, nopinene and dodecane are included, and the volume ratio of nonadecane, nopinene and dodecane is 1:3:3.
Embodiment 5
The present embodiment provides a kind of novel tarnished plant bug attractants, prepare the raw material packet of the novel tarnished plant bug attractant Nonadecane, nopinene and dodecane are included, and the volume ratio of nonadecane, nopinene and dodecane is 1:1:1.
Embodiment 6
The present embodiment provides a kind of novel tarnished plant bug attractants, prepare the raw material packet of the novel tarnished plant bug attractant Nonadecane, nopinene and dodecane are included, and the volume ratio of nonadecane, nopinene and dodecane is 1:5:5.
Embodiment 7
The present embodiment provides a kind of preparation methods of novel tarnished plant bug attractant, comprising the following steps:
Step 1: nonadecane, nopinene and dodecane are diluted to 1:1000,1:10000,1:1000 times of liquid respectively, it is standby With;
Step 2: nonadecane, nopinene and dodecane are pressed 1:(1-5): the volume ratio of (5-1) mixes.
Embodiment 8
The present embodiment provides a kind of application of novel tarnished plant bug attractant in trapping tarnished plant bug.
Embodiment 9
The present embodiment provides a kind of attractants, include above-mentioned novel tarnished plant bug attractant.
Novel tarnished plant bug attractant provided by the above embodiment is main component, energy using certain herbaceous plants with big flowers aldehyde and blatter alcohol The trap effect of attractant is enough greatly promoted, is brought convenience for the production of people, to promote crop yield.
Experiment one
31 kinds of active materials are identified from tarnished plant bug host and select wherein 16 volatile matter, to this 16 volatile matter and its again On the basis of carrying out odorant-binding protein and the Electroantennogram response research to tarnished plant bug adult with object, further progress field Trap effect is provided fundamental basis to develop the comprehensive control measures of tarnished plant bug.
1 material to be tested
1.1 experimental material
Every piece of colour table two sides of white insect-sticking plate is coated with the insect sticker of one layer of 0.4mm, and insect-sticking plate is the rectangle of 24cm × 40cm. Small test tube, bamboo pole, iron wire.
1.2 for agent of having a try
For agent of having a try: identifying 31 kinds of informationizations altogether in the host plant volatile of 7 kinds of tarnished plant bugs according to Sun Peng et al. Object is closed, this experiment is chosen 16 kinds of active materials and is used as agent of having a try, and is shown in Table 1.
N-compound in the test of table 1
Table 2-1 The standard chemical samples in the experiment
Field trap effect of 1.3 ten six kinds of plant volatiles to tarnished plant bug
Insect-sticking plate is white, and length and width are respectively and front and back sides have insect sticker, is tested in Tarim University, gardens, periphery Nursery base.The field tune other than any farming operations such as the spraying, topping of chemical pesticide and this test is not carried out during test It looks into, causes human interference to influence to avoid on test.Field before use, first by long wood pole it is fixed into the soil and keep hang down Directly, then with thin wire by insect-sticking plate it is fixed on above wood pole, is slightly above its bottom at the top of weeds, adjacent pole is between any two Distance be 15m, and insect-sticking plate has mucilage glue surface towards south.Haftplatte center hangs with 1ml and is compareed by the diluted active material of suet cream, this A little substances are placed in 1.5ml sample bottle, and bottleneck slopes slightly downward.The tarnished plant bug trapped on insect-sticking plate is signed after 3d Fixed and statistics.Each active material analog is repeated 6 times.The Duncan duncan's new multiple range method of DPS6.55 statistical software detects difference Conspicuousness.
1.4 ten six kinds of plant volatile species concentrations are with the field trap effect for comparing tarnished plant bug
NSC 77136, n-capric aldehyde, 3- ethylene -1- alcohol, docosane, nopinene various concentration, different ratio carry out field Trapping experiments.
2 results and analysis
The female male imago of 2.1 tarnished plant bugs is to 10-1(V/V) the field trap effect of the plant volatiles of concentration
It is 10 in 16 kinds of tarnished plant bug host plant volatile concentration of test-1(V/V) when, n-capric aldehyde is to female male imago Trap effect with compare paraffin phase than equal significant difference (P < 0.05).This experiment female adult gathering effect is bigger than male worm.
As a result as shown in Figure 1, wherein 1: tetratetracontane Tetratetracontan;2: octacosane Octa cosane;3: Heptacosane Heptacosan;4: nonadecane Nonadecan;5: stearmide Octadec anamid;6: spirolactone Spironolactone;7: heptadecane Heptadecan;8: n-capric aldehyde Decana lbeta;9: irisone 3-Buten-2- One, 4- (2,6,6-trimethy1-1-cyclohexen-1-yl)-;10:(Z)-butyric acid -3- hexene ester Butanoic acid, 3-hexenyl ester, (Z)-;11;Blatter alcohol 3-Hexen-1-ol,;12:(E) -4- hexen-1-ol 4-Hexen-1- Ol, (E);13;Dodecane Dodecane;14: nopinene á-Pinene;15:Z-3- methylbutanoic acid -3- hexene ester cis-3- Hexenyl isovalerate;16: pentadecane Pentadecane, data are average ± standard error in figure;Different letters are aobvious Show significant difference (Kruskal-Wallis Test, P < 0.05) between different plant volatiles.Fig. 2,3,4 are same.
The female male imago of 2.2 tarnished plant bugs is to 10-2(V/V) the field trap effect of the plant volatiles of concentration
As a result as shown in Fig. 2, note: data are average ± standard error;It is poor between the different plant volatiles of difference letter display Different significant (Kruskal-Wallis Test, P < 0.05).
It is 10 in 16 kinds of tarnished plant bug host plant volatile concentration of test-2(V/V) in, nopinene is to female male imago Trap effect with compare paraffin phase than equal significant difference (P < 0.05).This experiment female adult gathering effect is bigger than male worm.
The female male imago of 2.3 tarnished plant bugs is to 10-3(V/V) the field trap effect of the plant volatiles of concentration
It is 10 in 16 kinds of tarnished plant bug host plant volatile concentration of test-3(V/V) when, heptadecane, n-capric aldehyde, β-pinane Alkene, Z-3- methylbutanoic acid -3- hexene ester, pentadecane army to the trap effect of female male imago with compare paraffin phase than equal significant difference (P < 0.05), the every plate larva quantity amount of male worm have reached 4 or more.This experiment female adult gathering effect is bigger than male worm.
As a result as shown in figure 3, note: data are average ± standard error;It is poor between the different plant volatiles of difference letter display Different significant (Kruskal-Wallis Test, P < 0.05).
The female male imago of 2.4 tarnished plant bugs is to 10-4(V/V) the field trap effect of the plant volatiles of concentration
It is 10 in 16 kinds of tarnished plant bug host plant volatile concentration of test-4(V/V) when, stearmide, (Z)-butyric acid- 3- hexene ester, dodecane, nopinene to the trap effect of the female male imago of tarnished plant bug with compare paraffin phase than equal significant difference (P < 0.05).This experiment female adult gathering effect is bigger than male worm.
As a result as shown in figure 4, note: data are average ± standard error;It is poor between the different plant volatiles of difference letter display Different significant (Kruskal-Wallis Test, P < 0.05).
The preferable plant volatiles analysis of the female male imago trap effect of 2.5 tarnished plant bugs
In 16 kinds of 4 various concentrations of tarnished plant bug host plant volatile of the test of test, 8 kinds of trapping effects are filtered out altogether The preferable various concentration volatile matter of fruit, wherein mainly concentration is 10-3With 10-4(V/V) trap effect is better than other concentration. Most preferably heptadecane, the trapping borer population of certain herbaceous plants with big flowers aldehyde, female adult have reached 4/plate to trap effect, with other volatile matter significant differences (p <0.05)。
Table 1: to the female optimal plant volatiles of male imago trap effect of tarnished plant bug
Data are average ± standard error;Different letters show significant difference (Kruskal- between different plant volatiles Wallis Test, P < 0.05).Similarly hereinafter
The female male imago of 2.6 tarnished plant bugs matches field trap effect to different host plant volatile materials various concentrations
Using field mass trapping, four kinds of volatile materials various concentrations of Preliminary Determination, which are mutually matched, compares tarnished plant bug adult Gathering effect (being shown in Table 2).From the point of view of measurement result, tarnished plant bug female adult pest is than male worm to most proportion fields of volatile materials Between trap effect it is good, most proportion field trap effect response differences are significant (P < 0.05) in this experiment, wherein certain herbaceous plants with big flowers aldehyde 10-3:3- Hexen-1-ol 10-3(1:5), nonadecane 10-3: nopinene 10-4: dodecane 10-3(1:1:5), nonadecane 10-3: nopinene 10-4: Dodecane 10-3(1:5:1), good to the female male imago trap effect of tarnished plant bug, the every plate of female adult is 5 or more average, changes with other It closes object significant difference (P < 0.05).
Field trap effect of the 2. 5 kinds of volatile materials different ratios of table to the female male imago of tarnished plant bug
Note: data are average ± standard error;Different letters show significant difference (Kruskal- between different plant volatiles Wallis Test, P < 0.05).
3 brief summaries
This experiment has carried out the test of field trap effect to 16 kinds of volatile materials of tarnished plant bug host plant, filters out The plant volatile of various concentration, wherein the 10 of heptadecane, certain herbaceous plants with big flowers aldehyde and nopinene-3Trap effect is good.Plant volatiles are not With in concentration proportioning trap effect, certain herbaceous plants with big flowers aldehyde 10 is filtered out-3: blatter alcohol 10-3(1:5), nonadecane 10-3: nopinene 10-4: Dodecane 10-3(1:1:5), nonadecane 10-3: nopinene 10-4: dodecane 10-3(1:5:1) all has preferably female male imago Trap effect, every plate male worm trapping quantity reach 5 or more, these three proportions with other proportions in significant difference (P < 0.05)。
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, These modifications or improvements without departing from theon the basis of the spirit of the present invention are fallen within the scope of the claimed invention.

Claims (9)

1. a kind of novel tarnished plant bug attractant, it is characterised in that: the raw material for preparing the novel tarnished plant bug attractant includes Nonadecane, nopinene and dodecane, and the volume ratio of nonadecane, nopinene and dodecane is 1:(1-5): (5-1).
2. novel tarnished plant bug attractant according to claim 1, it is characterised in that: nonadecane, nopinene and dodecane Volume ratio be 1:1:5.
3. novel tarnished plant bug attractant according to claim 1, it is characterised in that: nonadecane, nopinene and dodecane Volume ratio be 1:5:1.
4. novel tarnished plant bug attractant according to claim 1, it is characterised in that: nonadecane, nopinene and dodecane Volume ratio be 1:3:3.
5. novel tarnished plant bug attractant according to claim 1, it is characterised in that: nonadecane, nopinene and dodecane Volume ratio be 1:1:1.
6. novel tarnished plant bug attractant according to claim 1, it is characterised in that: nonadecane, nopinene and dodecane Volume ratio be 1:5:5.
7. the preparation method of novel tarnished plant bug attractant described according to claim 1-any one of 6, which is characterized in that packet Include following steps:
Step 1: nonadecane, nopinene and dodecane are diluted to 1:1000,1:10000,1:1000 times of liquid respectively, it is spare;
Step 2: nonadecane, nopinene and dodecane are pressed 1:(1-5): the volume ratio of (5-1) mixes.
8. application of the novel tarnished plant bug attractant described according to claim 1-any one of 6 in trapping tarnished plant bug.
9. a kind of attractant, it is characterised in that: include novel tarnished plant bug attractant described in any one of claim 1-6.
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