CN108719282A - A kind of obligate attractant and control method of prevention oriental fruit months - Google Patents
A kind of obligate attractant and control method of prevention oriental fruit months Download PDFInfo
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- CN108719282A CN108719282A CN201810564660.0A CN201810564660A CN108719282A CN 108719282 A CN108719282 A CN 108719282A CN 201810564660 A CN201810564660 A CN 201810564660A CN 108719282 A CN108719282 A CN 108719282A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to attracting pests agent technical fields, provide a kind of obligate attractant and control method of prevention oriental fruit months, active ingredient is that sweet and sour wine liquid and host plant volatile mix, wherein sweet and sour wine liquid：Soft white sugar：Glacial acetic acid：Absolute ethyl alcohol：Water=3g:1ml:3ml:80ml；Host plant volatile：Aldehyde C-9, cis- 3- hexenes alcohol acetic ester, 6- methyl -5- hepten-2-ones,（Z）Ocimenum=14-β-：100：1：86, host plant volatile is dissolved as 1 μ g/ μ l with solvent hexane；Sweet and sour wine liquid and host plant volatile solution by volume 1:1 mixing.It is lured at a distance by food attractant and oviposition attractant short distance attracting action promotes chemical pesticide minimizing while ensureing control effect.Accelerate to promote green prevention and control product and technology.Conducive to preserving the ecological environment, ensuring food safety, ecological safety, it is conducive to the sustainable development of agricultural.
Description
Technical field
The invention belongs to attracting pests technical fields, and in particular to the obligate attractant of a kind of prevention oriental fruit months and anti-
Control method.
Background technology
With optimizing and revising for agricultural structure, fruit market has become the second largest agricultural product after grain market
Market.The sound development of fruit industry is most important to China's growth of agricultural efficiency, rural development, the realization of increasing peasant income target.Disease
Influence of the insect pest to production of fruit trees is very big.Northern China possesses the fallen leaves fruit trees cultivation area of large area, and peach, pears, apple are
Main Cultivation type, with oriental fruit monthsGrapholitha molesta(Busck) it is the harm of the moth fruit pest of representative
It is very serious, carry out destructive harm to fruit industrial zone.Oriental fruit months can endanger in the entire season of growth of peach, pears, apple
Evil its tender tip and fruit, the fruit of pears is endangered in the later stage in season, causes the peach folding tip, early stage shedding, gummosis to rot, the black cream of the operatic circle
Phenomena such as medicine etc., seriously affect fruit quality.In storing transportational process, the fruit being compromised is easily mouldy, rotten intolerant to storage
It is bad.Serious loss is caused to fruit industry.In the big generation of oriental fruit months, peach, pears concentrate the fruit area of plantation, hazard area
Up to 100%.For pear fruit rate up to 80%, the aggrieved rate of peach young sprout is up to 100% up to 95%, peach wormed fruit rate up to 65%, late-maturing peach.
Prevention of traditional chemical prevention and control method to oriental fruit months, not only preventive effect is excessively poor, but will bring pesticide residue,
Food security, killing natural enemy etc. a series of problem.Oriental fruit months is trapped and killed using biological source attractant, is current
One of common green prevention and control means.But widely used sex pheromone is largely trapped and killed and isotropic technology, just for male worm, to female
Worm lacks effective prevention and control.And female adult quantity is to the characteristic of the leading role and male worm multiple mating of field insect population so that
It is particularly important to the prevention and control of cytula female adult.
Invention content
The present invention provides a kind of prevention the obligate of oriental fruit months to lure control method, passes through the sweet and sour wine of food attractant
The remote of liquid is lured and the short distance of oviposition attractant host plant volatile is lured, synergistic, the small food of green prevention and control pears
Heart worm.This method can be carried out at the same time trapping to female male worm, female adult of especially becoming pregnant.While ensureing control effect, chemistry is promoted
The minimizing of pesticide.
The present invention is realized by following technical solution：A kind of obligate attractant of prevention oriental fruit months, active ingredient are
Sweet and sour wine liquid and host plant volatile mixing, wherein sweet and sour wine liquid is：Soft white sugar：Glacial acetic acid：Absolute ethyl alcohol：Water=3g:
1ml : 3ml : 80ml；Host plant volatile by following weight composition ratio material mixing：Aldehyde C-9, cis- 3- hexenols
Acetic acid esters, 6- methyl -5- hepten-2-ones,（Z）Ocimenum=14-β-：100：1：86, host plant volatile solvent hexane
Dissolving, a concentration of 1 μ g/ μ l；Sweet and sour wine liquid is 1 according to volume ratio with host plant volatile solution:1 ratio mixing.
The sweet and sour wine liquid is added to host plant volatile mixed solution in carrier centrifuge tube, and centrifuge tube both ends are close
It closes, the dosage of host plant volatile is 0.201mg in mixed solution.
The host plant volatile solution of the sweet and sour wine liquid of 200 μ l and 200 μ l are injected into centrifuge tube, sealed membrane sealing centrifugation
Nozzle；Using it is preceding with needle point centrifuge tube pipe lid prick an aperture after, hang on lure basin top away from 1 cm of the water surface at；April -10
Month, trapper hang in orchard it is ventilated in the ranks, away from the sturdy branch at 1.5 m of ground；Between each trapper
Away from 7.5 × 10 m；It replaces within every 3 days 1 time and is added with the obligate centrifuge tube for luring ingredient.It is described to lure basin for 25 cm of bore, depth
The red basin of plastics of 10cm, it is to lure the washing powder water of basin body product 2% to lure volume built in basin, is fixed with thin wire.
In the present invention, using sweet and sour wine liquid the characteristics of relatively equally can cause oriental fruit months olfaction reaction at a distance,
First oriental fruit months is lured near trapper.Then, oriental fruit months specificity is drawn using host plant volatile
Effect is lured, the oriental fruit months in trapper short distance, especially cytula female adult are lured in trapper.The two collaboration increases
Effect, dramatically increases and lures efficiency.
The present invention draws the telekinesy and oviposition of oriental fruit months using green prevention and control as theory, according to food attractant
Lure acts on the short distance of oriental fruit months, obtains a kind of effective obligate side of luring for preventing oriental fruit months
Method dramatically increases and lures efficiency, and the two is made to play more obvious action in the monitoring of oriental fruit months and mass trapping.It is protecting
While hindering control effect, the minimizing of chemical pesticide is promoted." it is basic that a control two the subtracts three " target for meeting Ministry of Agriculture's proposition, adds
It is fast to promote green prevention and control product and technology.Be conducive to preserve the ecological environment, ensure food safety, ecological safety, is conducive to agricultural
Sustainable development.
Sweet and sour wine liquid and host plant volatile are two kinds of can be carried out at the same time effectively to female male worm with larger potentiality
The attractant of prevention and control.As a kind of food attractant, sweet and sour wine liquid is that a kind of tradition is nuisanceless, control measure of broad spectrum activity, can
To trap and kill simultaneously various insects.But since insect itself is different to the ethyl alcohol of different ratio and the sensibility of acetic acid,
Cause sweet and sour wine liquid poor to target pest specificity.Sweet and sour wine liquid high volatility, can lure longer-distance insect close.It is special
The host plant volatile for determining ingredient and ratio is the oviposition attractant of a species specificity, can be directed to oriental fruit months male and female
Worm, especially cytula female adult, are prevented, and are trapped and killed it during female adult finds host plant oviposition.However,
In actual application process, due to there is the diffusion of host's volatile matter under the overall background of orchard, it is unfavorable for oriental fruit months is effective
It lures near trapper on ground.Two kinds of attractants are used in combination, and can play synergistic effect, first will by sweet and sour wine liquid
Main target pest --- oriental fruit months is lured near trapper, then by host plant volatile in specific manner by pears
Grapholita spp, especially cytula female adult, are lured in trapper.Draw in this way, luring and laying eggs at a distance by food attractant
Lure is closely lured, and is dramatically increased and is lured efficiency, so that the two is played in the monitoring of oriental fruit months and mass trapping more aobvious
The effect of work.
Description of the drawings
Fig. 1 is that the sweet and sour wine liquid field of different ratio lures moth situation；Fig. 2 is the host plant volatile of different proportion and dosage
The influence of female to oriental fruit months, the male moth amount of luring；Fig. 3 is the EAG response values of female, the male moth of oriental fruit months, in figure：PP is indicated
Host plant volatile, SS indicate sweet and sour wine liquid；Fig. 4 is the orientation reaction of flight rate of female, the male moth of oriental fruit months；Fig. 5 is pears
Grapholita spp is female, male moth flies to wind-tunnel 1/2 apart from the above reactivity；Fig. 6 is female, the male moth of oriental fruit months close to odor source
Within 10cm or landing reactivity；Fig. 7 is the obligate average induced moth number for luring method in field to oriental fruit months；Fig. 8 is special
Sex inducement method is in field to the induced moth number dynamic of oriental fruit months female adult；Fig. 9 lures method in field to the small food of pears to be obligate
The induced moth number dynamic of heart worm male worm；In figure：PP indicates that host plant volatile, SS indicate sweet and sour wine liquid；Figure 10 is obligate lures
Method lures situation in field to oriental fruit months.
Specific implementation mode
In order to make objects and advantages of the present invention be more clearly understood, the present invention is carried out with reference to embodiments further
It is described in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
Embodiment 1：The sweet and sour wine liquid of different ratio tests the female male worm field trap effect of oriental fruit months.Sweet and sour wine liquid
Different ratio is shown in Table 1.It is 3 by screening the acquisition sweet and sour wine liquid ratio best to the female male worm trap effect of oriental fruit months:
1 : 3 : 80。
1 sweet and sour wine liquid different ratio of table
The sweet and sour wine liquid of different ratio is in field to the trapping amount significant difference of oriental fruit months male worm（F=2.24, df=9,39, P
=0.047）, not notable to the trapping amount difference of female adult（F=1.889, df=9,39, P=0.093）.Wherein, female male worm is put down
Trapping amount reaches maximum in ratio 7（1.25 ± 0.48 and 24.00 ± 10.70）.Ratio 1,2 and 4 fails trapping and arrives
Female adult, the trapping amount of male worm is minimum in blank control.（Fig. 1）
Embodiment 2：The host plant volatile of different proportion and dosage is female to oriental fruit months, male moth field trap effect shadow
Ring experiment.Host plant volatile ratio is shown in Table 2 with dosage.Luring the female male worm of oriental fruit months for specificity is obtained by screening
It is aldehyde C-9 to catch the best host plant volatile ratio of effect and dosage：Cis- 3- hexenes alcohol acetic ester：6- methyl -5- heptene -
2- ketone：（Z）Ocimenum=14-β-: 100 ：1 ：86 proportioning, 0.201mg dosage.
2 host plant volatile ratio of table and dosage
The field moth trapping of host plant volatile component difference under ratio and the collective effect of dosage is not notable（F =
0.560, df= 6, 56, P= 0.76）, same difference is not notable under independent ratio or dosage effect（Dosage：F =
0.217,df = 1, P= 0.446；Ratio：F = 1.317, df = 6, P= 0.271）.For dosage, 0.1 ×
Induced moth number highest, 0.01 × induced moth number it is minimum.For ratio, the induced moth number of R2 is higher than R1.In all proportionings, it is in dosage
0.1 × when, R2（14.5 ± 6.96）Induced moth number highest.（Fig. 2）
Embodiment 3：Sweet and sour wine liquid, host plant volatile and the obligate of the two combined use lure method to oriental fruit months
Lure the contrast test of situation.It utilizes tentaculum electric potential to detect feeler to the reactivity of smell indoors, passes through wind tunnel simulation field
Between activity space measure smell induction generate flight behavior, finally field carry out trap effect inspection.Oriental fruit months
The EAG response values of female, male moth are shown in Fig. 3.The orientation reaction of flight rate of female, the male moth of oriental fruit months is shown in Fig. 4.Oriental fruit months is female,
Male moth flies sees Fig. 5 to wind-tunnel 1/2 apart from the above reactivity.Female, the male moth of oriental fruit months close within odor source 10cm or
Landing reactivity is shown in Fig. 6.It is obligate that method is lured to lure moth situation to see Fig. 7-9 oriental fruit months in field.In figure：PP expressions are posted
Main plant volatile, SS indicate sweet and sour wine liquid.From content in figure：Sweet and sour wine liquid can cause oriental fruit months feeler strong
Olfaction reaction.In wind-tunnel, environmental field is relatively small, and host plant volatile causes the orientation reaction of flight of female adult and flies
It is better than sweet and sour wine liquid apart from the above reaction to wind-tunnel 1/2, the two effectively raises oriental fruit months female adult after being used cooperatively
Orientation flight flies to wind-tunnel 1/2 apart from above and close within odor source 10cm or the reactivity landed.It sweet and sour wine liquid and posts
The synergistic function of main plant volatile effectively raises obligate decoy in field and draws to the female male worm of oriental fruit months
Lure ability.
The EAG response values that oriental fruit months is female, sweet and sour wine liquid, host plant volatile and the two is used in combination in male moth
Difference is notable（It is male：F=22.453, df=2,20, P<0.05；It is female：F=30.883, df=2,20, P<0.05）.Wherein,
The EAG response value highests that the two is used in combination in male worm feeler（3.40±0.22mV）.Female adult feeler individually makes sweet and sour wine liquid
With（6.90±0.31mV）And the two is used in combination（6.10±0.21mV）EAG response values be higher than host plant volatile list
Solely use（Fig. 3）.In wind tunnel behavior reaction, female adult is close within odor source 10cm or the behavioral difference of landing is notable（H=
12.645, df=2, P=0.02）, remaining indicator difference is notable（Orientation flight, it is female：F=2.122, df=2,17, P=
0.154, it is male：H=1.654, df=2,P=0.437；Fly to wind-tunnel 1/2 more than, it is female：H=4.726, df=2,P=
0.094, it is male：H=4.235, df=2,P=0.120；Close within odor source 10cm or land, it is male：H=2.528, df=2,P=
0.283）.The orientation of female male worm is flown（35.00 ± 8.85% and 10.00 ± 5.16%）, fly to wind-tunnel 1/2 more than（25.00
± 8.47% and 20.00 ± 8.56%）And female adult close within odor source 10cm or is landed（3.33±2.11%）Behavior it is anti-
Should highest be reached when the two is used in combination（Fig. 4-6）.In the moth trapping contrast test of field, the two is used in combination to female
The trapping amount of male worm is higher than exclusive use（It is female：H=2.829, df=4,P=0.587；It is male：H=8.629, df=4,P=
0.071）, female adult averagely trapping amount is 9.25 ± 7.04, and male worm averagely trapping amount is 73.25 ± 52.05（Fig. 7）.Field
In induced moth number dynamic, August 8 days the above two be used in combination to the trap effect of female male worm less than both exclusive use, but August
The trap effect being used in combination both after 8 days, which is persistently higher than, to be used alone（Fig. 8-9）.
Claims (4)
1. a kind of obligate attractant of prevention oriental fruit months, it is characterised in that：Active ingredient is sweet and sour wine liquid and host plant
Volatile matter mixes, wherein sweet and sour wine liquid is：Soft white sugar：Glacial acetic acid：Absolute ethyl alcohol：Water=3g: 1ml : 3ml : 80ml；It posts
Main plant volatile by following weight composition ratio material mixing：Aldehyde C-9, cis- 3- hexenes alcohol acetic ester, 6- methyl -5- heptan
Alkene -2- ketone,（Z）Ocimenum=14-β-：100：1：86, host plant volatile is dissolved with solvent hexane, a concentration of 1 μ g/ μ l；
Sweet and sour wine liquid is 1 according to volume ratio with host plant volatile solution:1 ratio mixing.
2. a kind of obligate attractant of prevention oriental fruit months according to claim 1, it is characterised in that：The sweet and sour wine
Liquid is added to host plant volatile mixed solution in carrier centrifuge tube, and centrifuge tube both ends are closed, and host plants in mixed solution
The dosage of object volatile matter is 0.201mg.
3. utilizing the method for the obligate attractant prevention oriental fruit months described in claims 1 or 22, it is characterised in that：By 200 μ l
Sweet and sour wine liquid and 200 μ l host plant volatile solution inject centrifuge tube, sealed membrane sealing centrifugation nozzle；Needle is used before use
Point centrifuge tube pipe lid prick an aperture after, hang on lure basin top away from 1 cm of the water surface at；October in April-, trapper hang on fruit
In garden it is ventilated in the ranks, away from the sturdy branch at 1.5 m of ground；Each 7.5 × 10 m of trapper spacing；Every 3 days
It replaces 1 time and is added with the obligate centrifuge tube for luring ingredient.
4. the method according to claim 3 for preventing oriental fruit months using obligate attractant, it is characterised in that：It is described to lure
Basin is the red basin of plastics of 25 cm of bore, depth 10cm, and it is to lure the washing powder water of basin body product 2% to lure volume built in basin, uses thin wire
It is fixed.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113498780A (en) * | 2021-06-21 | 2021-10-15 | 河北省农林科学院昌黎果树研究所 | Paste for efficiently inducing and controlling oriental fruit moth and preparation method thereof |
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