CN110419532A - Attractant is integrated in a kind of field for preventing and treating oriental fruit months - Google Patents

Attractant is integrated in a kind of field for preventing and treating oriental fruit months Download PDF

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Publication number
CN110419532A
CN110419532A CN201910735597.7A CN201910735597A CN110419532A CN 110419532 A CN110419532 A CN 110419532A CN 201910735597 A CN201910735597 A CN 201910735597A CN 110419532 A CN110419532 A CN 110419532A
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China
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attractant
oriental fruit
fruit months
field
moth
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孔维娜
马瑞燕
王怡
郭永福
李捷
柴晓晗
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INSTITUTE OF PLANT PROTECTION SHANXI ACADEMY OF AGRICULTURE SCIENCE
Shanxi Agricultural University
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INSTITUTE OF PLANT PROTECTION SHANXI ACADEMY OF AGRICULTURE SCIENCE
Shanxi Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to attracting pests agent technical fields; attractant is integrated for a kind of field for preventing and treating oriental fruit months is optimal; including sex pheromone, food bait and protection component; sex pheromone is according to mass ratio are as follows: cis- 8- dodecylene alcohol acetate: trans- 8- dodecylene alcohol acetate: cis- 8- lanolin alcohol: 12 carbon -1- alcohol=100:6-7:15-20:2-7 composition; food bait includes terpinyl acetate, and protection group is divided into honey and antioxidant；The attractant effectively reduces oriental fruit months population and its harm in different peach gardens, female moth of especially becoming pregnant；It is the present invention to effectively integrate sex attraction agent, while ensureing control efficiency, promotes chemical pesticide minimizing, accelerate to promote green prevention and control product and technology, conducive to preserving the ecological environment, ensuring food safety, ecological safety, conducive to the sustainable development of agricultural.

Description

Attractant is integrated in a kind of field for preventing and treating oriental fruit months
Technical field
The invention belongs to attracting pests technical fields, and in particular to effectively integrate sex pheromone and food bait simultaneously significant Improve the attractant of field control oriental fruit months.
Background technique
Influence of the pest and disease damage to production of fruit trees is very big, wherein causes harm by the moth fruit pest of representative of oriental fruit months It is the most serious, destructive harm is brought to fruit industry.Oriental fruit months is distributed widely in all over the world, can be in peach, pears, apple The entire season of growth of fruit causes harm its tender tip and fruit, causes harm the fruit of pears in the later period in season.It causes the peach folding tip, fall in early days Fruit, gummosis rot, the operatic circle black plaster etc..Seriously affect fruit quality.In the big generation of oriental fruit months, peach, pears concentrate kind The area Zhi Guo, area of causing harm is up to 100%.Pear fruit rate is up to 80%, and the aggrieved rate of peach young sprout is up to 95%, peach wormed fruit rate up to 65%, late-maturing peach Up to 100%.Used traditional chemical prevention and control method in production of fruit trees need to carry out 7-8 application every year.It averagely sprays per acre It is 400 jin of mist amount or so, but often not fully up to expectations to the preventive effect of oriental fruit months.Since larva is caused harm with brill moth and hidden Property, conventional chemical pesticide preventive effect is excessively poor, but will bring pesticide residue, food safety, killing natural enemy etc. are a series of to ask Topic.Oriental fruit months is trapped and killed using biological source attractant, is one of currently used green prevention and control means.But property letter Plain Prevention Technique is ceased, just for male worm, effective prevention and control are lacked to female adult.And female adult quantity is to the leading work of field insect population With the characteristic with male worm multiple mating, so that particularly important to the prevention and control of cytula female adult.
Tropical area and Mate choice play a crucial role the procreation of plant-feed insect population, and information chemical combination Object plays critical effect during adjusting heart-eating worm selection host and spouse again.Species of insect pests based on infochemical The monitoring and control strategy of group opens a new and effective control of insect approach.Insect sex pheromone and host plant are waved Volatile material, which combines, to provide more complete information to find spouse insect, not only indicate the presence of anisotropic insect, but also Also illustrate the presence of host.So, insect host and the substance of non-host influence the sex pheromone behavior of insect, this is in pest It is of great significance in integrated management.In orchard ecosystem, insect bisexuality must exist jointly could mating oviposition. It by insect's food-taking, lays eggs and the semiochemical being related to of seeking a spouse is integrated, is widely used for various pests, especially host Specialization is compared with strong and/or poor flight performance caste.In recent years, both at home and abroad to sex pheromone of Grapholitha molesta, host The progress of plant volatile, sweet and sour wine liquid, food bait etc. quickly and is constantly brought forth new ideas, and especially sex pheromone prevents and treats skill Art has been widely used for population monitoring and large area comprehensive treatment.But oriental fruit months integrates attractant in field at present Still to lure based on male worm, female adult proportion is extremely low, and virgin's female adult is almost lured less than this technology upgrading increases in practical applications The effect of effect is not obvious.Therefore, by EAG reaction, wind tunnel behavior measurement and field lure moth, specify oriental fruit months it is female, Male moth acts on the individual behavior of different attractants, and adds medium and antioxidant, and dosage form is integrated in determination, then in different peach gardens Its implementation result is verified, this has certain directive function to novel attractant of the exploitation based on insect sex pheromone.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, propose a kind of for preventing and treating the field for integrating attractant of oriental fruit months Between be formulated, improve stability and extend the lasting period, suitable for preventing and treating the different generations oriental fruit months in different peach gardens.
The technical scheme adopted by the invention to solve the technical problem is that.
Attractant, including sex pheromone, food bait and protection group are integrated in a kind of field for preventing and treating oriental fruit months Point, the sex pheromone is according to mass ratio are as follows: cis- 8- dodecylene alcohol acetate: trans- 8- dodecylene alcohol acetate: cis- 8- lanolin alcohol: 12 carbon -1- alcohol=100:6-7:15-20:2-7 composition, the food bait includes acetic acid pine tar Ester, the protection group are divided into honey and antioxidant.
Preferably, the sex pheromone is according to mass ratio are as follows: cis- 8- dodecylene alcohol acetate: trans- 8- dodecylene Alcohol acetate: cis- 8- lanolin alcohol: 12 carbon -1- alcohol=100:6.8:19.1:5.4 composition.
Preferably, the volume ratio of the sex pheromone and food bait is 1:0.8-1.2.
Preferably, the food bait further includes the water of non-ionic wetting agent and 30-40 DEG C.
More preferably, the non-ionic wetting agent is any one in Span, Tween, APG.
Preferably, the volume of the honey is the 0.015-0.025% of volume after sex pheromone and food bait mixing.
Preferably, the antioxidant is 2,6-Di-tert-butyl-4-methylphenol or Propyl Gallate。
Preferably, the quality of the antioxidant is gross mass after the sex pheromone, food bait and honey mix 30-40%.For the present invention as most preferably component is lured, sex pheromone plays governing effect in integrating attractant, lures table to male moth Now best, food bait, as the synergetic effect additive of sex pheromone, plays low coverage to male moth and female moth in mixed attractant From the effect lured, sex pheromone and food bait solution are mixed according to the ratio that volume ratio is 1:1.As best protection group Point, medium selects the honey of 10 μ l, the BHT of antioxidant selection 30% extends the duration, and increase induced moth number.It will most It is good lure and protect component constitute it is optimal integrate attractant different peach gardens to the trappability of different generations oriental fruit months compared with Height, the female moth lured are female moth of becoming pregnant, and the folding tip rate in four peach gardens and borer fruit rate significantly reduced respectively to 1.26 ± 0.13% and 0.12 ± 0.1%.
In the present invention, sex pheromone, host plant volatile, sweet and sour wine liquid, food bait are to oriental fruit months in orchard In action pathway: the female adult pest feeler just sprouted wings first detects food (tentaculum electric potential), flies to host (wind-tunnel), with male worm quilt (field lures moth) is found at suitable oviposition after dynamic mating；The male worm feeler just sprouted wings detects the female adult pest (touching near host Angle current potential), it flies to female adult pest (wind-tunnel), aggressive and female adult pest mating (field lures moth).Oviposition attractant we select It is the volatile matter of the peach tip, sweet and sour wine liquid is related with fruit maturity.If carrying out Field information, entire peach garden all disperse tender tips With the volatile matter of fruit.Therefore, under host plant volatile, sweet and sour the wine liquid feature suitable with overall background smell, the present invention Selection utilization food bait lures female adult feeding, and lures male worm to mate using sex pheromone, i.e. sex pheromone and food bait It mixes the oriental fruit months completion wind tunnel behavior after promoting to detect host and field lures the two committed steps of moth.
Ideal attractant should keep the validity discharged for a long time, active constituent not wasted, in production and Field information Inexpensively, and it is nontoxic.Especially, ultraviolet radiation is avoided to make active ingredient degradation.The volatilization of the attractant of most of Lepidoptera Property it is different, and field regulating ratio is again quite accurate.Therefore, the dosage form of insect attractant must just have constant homogeneous release Feature.So, a kind of feasibility of novel oriental fruit months mixture attractant dosage form is explored, principle is according to small molecule The characteristics of pheromone compound can equably be discharged with the volatile component in finish altogether, it is special to reach efficient constant release Point.In the present invention, it is integrated in oriental fruit months and is added to best medium and antioxidant in attractant, in long-term use process Middle induced moth number is high and effect stability and lasting period are long, suitable with commercially available property lure effect.
The difference of the factors such as geography, layout, environment, plantation type, plantation density, orchard worker's management in orchard is lured various The application of agent influences significant.In the present invention, oriental fruit months optimum integration attractant peach garden surrounding enviroment (such as: with jujube tree, The companion plantings such as corn), landform, orchard worker manage under degree, the plantation otherness of factors such as density degree, can be small to different generations pears Heart-eating worm carries out mass trapping, female moth of especially becoming pregnant.Although the female moth of oriental fruit months only mates once, its egg laying amount is most 68 can be produced, 8 can be produced at least；The hatching rate of ovum is at least at most 12.5% up to 79.0%.If permanently effective trapping Female moth can be by the population for significantly reducing oriental fruit months and harm caused by it.Although four peach gardens there are height above sea level, layout, The difference of landform, plantation type etc., but after integration attractant long-time service, the danger of oriental fruit months can be greatly lowered Evil.It can be seen that the integration attractant has field feasibility, different regions can be used in and promoted the use of.
The present invention is generated compared with the existing technology to be had the beneficial effect that.
The present invention is using green prevention and control as theory, according to the Reproductive Strategy of insect, under the collective effect of sex attraction agent, increases Add the probability that meets of male with female, and obtains the place of extra-nutrition, mating, oviposition, so that the chance of both sexes mating is improved, A kind of effective integration attractant for being used to prevent and treat oriental fruit months is obtained, dramatically increases and lures efficiency.To different generations The trappability of oriental fruit months is higher, and the female moth lured is female moth of becoming pregnant, and the rate that causes harm significantly reduces, and is ensureing prevention and treatment effect While fruit, the minimizing of chemical pesticide is promoted." it is basic that a control two the subtracts three " target for meeting Ministry of Agriculture's proposition, accelerates to promote green Color prevention and control product and technology.Be conducive to preserve the ecological environment, ensure food safety, ecological safety, is conducive to the sustainable of agricultural Development.
Detailed description of the invention
Fig. 1 is that the oriental fruit months of comparative experiments example 1 is female, male moth is to the different EAG response values for integrating attractant.
Fig. 2 is that the oriental fruit months of comparative experiments example 1 is female, male moth is to the different orientation flight behavior reactions for integrating attractant Rate.
Fig. 3 is that the oriental fruit months of comparative experiments example 1 is female, male moth is to the different flight behavior reactions of turning back for integrating attractant Rate.
Fig. 4 is that the oriental fruit months of comparative experiments example 1 is female, male moth is to the different vertical rotary behavior reactions for integrating attractant Rate.
Fig. 5 be the oriental fruit months of comparative experiments example 1 is female, male moth to it is different integrate attractant close to odor source 10cm with Interior or landing behavior reaction rate.
Fig. 6 is the different trapping situations for integrating attractant in field wintering for adult occurrence in peak period to oriental fruit months.
Fig. 7 is the different trapping situations for integrating attractant in field first generation adult occurrence in peak period to oriental fruit months.
Fig. 8 is the different trapping situations for integrating attractant in field second generation adult occurrence in peak period to oriental fruit months.
Fig. 9 is the different trapping situations for integrating attractant in field third generation adult occurrence in peak period to oriental fruit months.
Figure 10 is that the best of oriental fruit months lures field moth trapping of the component under different medium and its dosage.
Figure 11 be oriental fruit months most preferably lure various concentration antioxidant is added in component and medium after field it is flat Lure the effect of female moth amount.
Figure 12 be oriental fruit months most preferably lure various concentration antioxidant is added in component and medium after field it is flat Lure the effect of male moth amount.
Figure 13 be oriental fruit months most preferably lure various concentration antioxidant is added in component and medium after field it is flat Lure the female moth amount lasting period.
Figure 14 be oriental fruit months most preferably lure various concentration antioxidant is added in component and medium after field it is flat Lure the male moth amount lasting period.
Figure 15 lures situation to the oriental fruit months of different peach garden differences generation phase for optimal attractant of integrating
Figure 16 is to identify the optimal dissection for integrating the different female moths of peach garden oriental fruit months that timber quarantine arrives.
Figure 17 is that folding tip rate caused by different peach gardens and different generations oriental fruit months compares.
Figure 18 is that borer fruit rate caused by different peach gardens and different generations oriental fruit months compares.
In figure: PH indicates that sex pheromone, PP indicate that host plant volatile, SS indicate that sweet and sour wine liquid, FL indicate that food lures Bait.
Specific embodiment
In order to which technical problems, technical solutions and advantages to be solved are more clearly understood, in conjunction with reality Example and attached drawing are applied, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to It explains the present invention, is not intended to limit the present invention.Below with reference to the examples and drawings technical solution that the present invention will be described in detail, but The scope of protection is not limited by this.
Embodiment 1
A kind of optimal integration attractant for preventing and treating oriental fruit months is made of sex pheromone, food bait and protection component, as Component is most preferably lured, sex pheromone is cis- 8- dodecylene alcohol acetate: trans- 8- dodecylene alcohol acetate according to mass ratio: Cis- 8- lanolin alcohol: 12 carbon -1- alcohol=100:6.8:19.1:5.4 composition configure 524 μ g, in integrating attractant altogether Play governing effect, put up the best performance to luring for male moth, food bait by 24.25mL terpinyl acetate, the APG of 0.75mL and 25mL35 DEG C of warm water is made, and as the synergetic effect additive of sex pheromone in mixed attractant, plays to male moth and female moth The effect closely lured mixes sex pheromone solution and food bait solution according to the ratio that volume ratio is 1:1；As most Good protection component, medium select the honey of 10 μ l, and antioxidant selects the 2,6-Di-tert-butyl-4- of gross mass 30% Methylphenol(BHT), that is, the duration is extended, and increases induced moth number.
Comparative experiments example 1
The sex pheromone of acquired field optimum formula, host plant volatile, sweet and sour wine liquid, food bait combination are small to pears Heart-eating worm lures the comparative test of situation.The formula of four kinds of attractants is shown in Table 1.Feeler pair is detected using tentaculum electric potential instrument indoors The reactivity of smell measures the flight behavior that smell induction generates by wind tunnel simulation field activity space, finally in field Carry out the inspection of trap effect.
Oriental fruit months is female, male moth sees Fig. 1 to the different EAG response values for integrating attractant.
Oriental fruit months is female, male moth sees Fig. 2-5 to the different different behavior reaction rates for integrating attractant.
Difference integrates attractant and sees Fig. 6-9 to the trapping situation of oriental fruit months in field difference generation period.
In figure: PH indicates sex pheromone, and proportion and ingredient are configured according to embodiment 1, PP indicates host plant volatile, SS Indicate that sweet and sour wine liquid, FL indicate food bait.From content in figure: epigamic of the PH as the raw survey indoors of single attractant Preferably, and in field PH the effect mixed with other attractants is similar, and governing effect is played in integrating attractant, is mainly acted on Male moth.FL is only acted on obviously as single attractant in wind-tunnel, and effect is more preferable under three kinds of determination conditions after integrating with PH, As the synergist of PH in mixed attractant, it is mainly shown as and closely lures, both lured male moth or lured female moth.PH is to hero Luring for moth is put up the best performance, and FL plays the role of closely luring to male moth and female moth, and by the two, 1:1 mixes work by volume Component is lured to integrate the best of attractant.
Difference integrates the EAG reaction that attractant is responsible for female, the male moth of oriental fruit months, and the extent of reaction of female moth is significantly high In male moth, and the different EAG values for integrating attractant differ greatly (♀: H=68.131, df=13, P < 0.05, ♂: H= 79.762, df = 14, P < 0.05；Fig. 1).Wherein, male moth is to PP+SS+FL(4.2 mV) > PH+PP+SS(3.2 mV) > PP+SS(3.2 mV) > PP+PH+FL+SS(3.0 mV) > PH+SS+FL(2.7 mV) > PH+PP(2.3 mV) > SS(2.01 MV reaction) is stronger；And female moth is to SS(6.9 mV) > PP+SS(5.9 mV) > PP+SS+FL(5.8mV) > PH+SS+FL (4.2 mV) > PH+PP+SS(4.2 mV) > PH+PP(3.7 mV) > PP(3.5 mV) > PP+FL(3.3 mV) > PP+PH+ FL+SS(3.2 mV) reaction it is higher, hence it is evident that integrate attractant higher than other.
Oriental fruit months is female, male moth is not significant to each behavioral difference for integrating attractant in wind-tunnel, (orientation flight (♀: H=24.175, df=15, P=0.062；♂: H=18.469, df=15, P=0.239), flight of turning back (♀ and ♂: H=21.068, df=15, P=0.135), vertical rotary (♀ and ♂: H=17.267, df=15, P =0.303), close within 10 cm of odor source or land (♀ and ♂: H=19.366, df=15, P=0.198)；Fig. 2- 5), and female moth is above male moth to the behavior reaction rate for integrating attractant.Orientation of the female moth to PH+PP+SS+FL, FL and PH+FL Flight rate is 53.3 ± 12.0%, 50 ± 8.6% and 40 ± 5.2%, flight of turning back to PH+PP+SS+FL, PP+SS and PH+PP+FL Rate is 35 ± 12.3%, 30 ± 7.3% and 28.3 ± 7.5%, is around core specific rotation to PH+PP+FL, PH+PP+SS+FL and FL 13.3 ± 4.9%, 11.7 ± 6.5% and 11.7 ± 4.8%, and to FL, PP+SS and PH+PP+SS close to odor source 10cm with Probability that is interior or landing is 8.3 ± 4.0%, 3.3 ± 2.1% and 3.3 ± 2.1%；Male moth flies to the orientation of PH, FL and PH+FL Rate is 30.0 ± 9.2%, 30.0 ± 6.3% and 26.7 ± 7.6%, is 26.7 ± 8.6% and 13.3 to the flight rate of turning back of PH and FL It ± 6.3%, is 5.0 ± 3.4%, 3.3 ± 3.4% and 3.3 ± 3.3% around core specific rotation to SS, FL and PH+FL, and to PH+PP With PH+PP+SS+FL within 10 cm of odor source or the probability that lands is 3.3 ± 2.1%.
In addition to overwinter generation, difference integrates attractant and lures effect to oriental fruit months in other three generations adult occurrence in peak period Significant (the overwinter generation: H=15.529, df=16, P=0.486 of fruit difference；The first generation: H=33.158, df= 16, P < 0.05；The second generation: H=38.516, df=16, P < 0.05；The third generation: H=31.847, df=16, P < 0.05；Fig. 6-9).Although difference integrates attractant and shows attracting action to female moth in four generations, hero is lured The quantity of moth is significantly higher than female moth.The phase occurs in winter generation adult, the average induced moth number of 10 d is more than 60 integration attractants For FL+SS(100.0 ± 41.7), FL(79.0 ± 8.9) and PH+FL+SS(68.8 ± 8.4), and lured less than 20 integration Agent is PP(18.8 ± 4.0)；In first generation adult stage, the average induced moth number of 10 d is more than that 280 integration attractants are PH(372.0 ± 109.2), PH+FL(322.5 ± 105.3) and PH+PP(288.8 ± 72.3), and lured less than 20 integration Agent is SS(7.0 ± 2.4)；In second generation adult stage, the average induced moth number of 10 d is more than that 90 integration attractants are PH+ PP+SS+FL(142.0 ± 85.5) and PP+FL+SS(97.0 ± 39.0), and less than 10 integration attractant be SS(6.3 ± 2.4) and FL(4.8 ± 2.4)；In third generation adult stage, the average induced moth number of 10 d is more than that 60 integration attractants are PP+SS(82.5 ± 59.1), PH+FL+SS(68.0 ± 7.6) and PH+FL(61.3 ± 6.6), and lured less than 10 integration Agent is SS(6.3 ± 3.7) and PP+FL(2.8 ± 1.3).Therefore, although every generation lures the effect of oriental fruit months is optimal to draw Lure is different, is mainly shown as FL+SS, PH, PH+FL, PH+FL+SS, PH+PP+SS+FL, PH+PP+SS, PP+FL+SS, PP+ The moth trapping of SS, PH+PP, FL are good.
Comparative experiments example 2
Using the attractant of embodiment 1 as sample, carries out oriental fruit months and the field control of component is most preferably lured to test.Most preferably drawing It lures and adds medium and antioxidant in component, specify the protection component for integrating attractant.Oriental fruit months most preferably lures group The field moth trapping under different medium and its dosage is divided to see Figure 10.In most preferably luring in component and medium for oriental fruit months Field moth trapping and lasting period see Figure 11-14 after addition various concentration antioxidant.The content from figure is it is found that medium selection 10 The honey of μ l, the 2,6-Di-tert-butyl-4-methylphenol(BHT of antioxidant selection 30%), both extend and continue Phase, and induced moth number is increased, as the protection component for integrating attractant.
5 μ l, 10 μ l, the honey of 15 μ l, cottonseed oil, rapeseed oil, corn oil conduct are added in component respectively most preferably luring When medium, various dose moth trapping difference under different medium it is not significant (F=0.773, df=12,80, P= 0.675；Figure 10).For medium, the induced moth number difference of different medium not significant (F=0.792, df=3, P=0.503； Figure 10), but under same media, induced moth number significant difference (F=3.011, df=3, P < 0.05 of various dose；Figure 10), Induced moth number (29.3 ± 17.8) is maximum when using the honey of 10 μ l as medium, the induced moth number (the 7.0 when cottonseed oil of 10 μ l is medium ± 3.6) minimum.
Most preferably lure middle addition various concentration in component and medium (30%, 35% and BHT 40%), 40%BHT lure moth Amount be significantly higher than other concentration and control (♀: F=7.792, df=4,19, P < 0.05, ♂: F=8.227, df= 4, 19, P < 0.05；Figure 11-12), and the quantity of male moth is lured to be significantly higher than female moth.In addition, as illustrated in figs. 13-14, from June 20 started, and for female moth, the moth trapping of 30%BHT is preferable before July, to July 20 before (peak period) most The good moth trapping for luring component is preferable, and the moth trapping of 30% and 40%BHT omits (between two peak periods) before arriving August 4th Good, before arriving August 24th (peak period), the moth amount of 30%BHT is obviously increased, and after August 24, only 35%BHT can lure a small amount of Female moth；For male moth, the induced moth number that BHT is not added with before August 4th, which is consistently higher than, is added to BHT, after August 4th, The induced moth number of 30 %BHT is significantly higher than other processing and control.
Comparative experiments example 3
Using the attractant of embodiment 1 as sample, the optimal Field information effect for integrating attractant of oriental fruit months is carried out.In difference Peach garden has evaluated the optimal implementation result for integrating attractant.The optimal small food of pears integrated attractant and different peach garden differences with the phase occur Heart worm lures situation to see Figure 15.To it is optimal integrate timber quarantine to the different female moths of peach garden oriental fruit months dissection identification see Figure 16 and table 2.Folding tip rate and borer fruit rate, which compare, caused by different peach gardens and different generations oriental fruit months sees Figure 17 and 18.From figure It is found that oriental fruit months is optimal integrates attractant and lure moth rate higher in four peach gardens, the female moth lured is middle content It becomes pregnant female moth, and the folding tip rate in four peach gardens and borer fruit rate all significantly reduce, and roll over tip rate minimum 1.26% ± 0.13%, moth Fruit rate minimum 0.12% ± 0.1%.
It is optimal integrate attractant under the action of, the moth rate that lures at Dongshan bottom reaches as high as 81.80%, Western Hills bottom and well mind Moth rate is lured secondly, respectively 70.00% and 79.90%.Well mind average trapping amount up to 468.25 ± 95.40 (Figure 15). The female moth that 4 peach gardens are traped is taken back laboratory dissection discovery, and it is become pregnant female moth (see Figure 16 and table 2).In larva When a generation occurs, tip rate highest is rolled at Dongshan bottom, up to 4.33%.When the second generation occurs, a fort folding tip rate highest is passed through, has been reached 8.50%.When the third generation occurs, it is equal with the folding tip rate of well mind to pass through a fort up to 8.09% for Dongshan bottom folding tip rate highest.Western Hills bottom Folding tip rate it is minimum always in the three generations of oriental fruit months, respectively 1.26%, 1.73%, 1.68%(Figure 17).The first of larva When in generation, occurs, the borer fruit rate highest at Western Hills bottom, up to 22.00%.When the second generation occurs, the borer fruit rate highest of well mind has reached 14.67%.When the third generation occurs, the borer fruit rate highest at Dongshan bottom, up to 3.00%.And a fort is passed through in the whole of oriental fruit months larva A generation borer fruit rate is all minimum (Figure 18).
It is available to draw a conclusion by the above comparative experiments: by sex pheromone solution (cis- 8- dodecylene alcohol acetate: Trans- 8- dodecylene alcohol acetate: cis- 8- lanolin alcohol: 12 carbon -1- alcohol=100:6.8:19.1:5.4,524 μ g) 1:1 is mixed by volume with food bait solution (24.25mL terpinyl acetate, 0.75mL non-ionic wetting agent and 25mL warm water) It closes, formation most preferably lures component, and the different distance for efficiently accomplishing both sexes is lured；Its add 10 μ l honey and 30% 2,6- Di-tert-butyl-4-methylphenol, forms best protection component, effectively extends the lasting period and then improves induced moth number；It obtains Lure and protect component formed it is optimal integrate attractant, which effectively reduces the oriental fruit months kind in different peach gardens Group and its harm, female moth of especially becoming pregnant.It most preferably will lure and protect component to constitute optimal attractant of integrating in different peach gardens pair The trappability of different generations oriental fruit months is higher, and the female moth lured is female moth of becoming pregnant, and the harm in four peach gardens Rate all significantly reduces, and rolls over tip rate minimum 1.26 ± 0.13%, borer fruit rate minimum 0.12 ± 0.1%.
According to the Reproductive Strategy of insect, sex attraction agent is effectively integrated, and carries out field control and verifying, is obtained optimal whole The Field information formula for closing attractant promotes chemical pesticide minimizing while ensureing control efficiency.Accelerate to promote green anti- Control product and technology.Conducive to preserving the ecological environment, ensuring food safety, ecological safety, conducive to the sustainable development of agricultural.
Embodiment 2
A kind of optimal integration attractant for preventing and treating oriental fruit months is made of sex pheromone, food bait and protection component, as Component is most preferably lured, sex pheromone is cis- 8- dodecylene alcohol acetate: trans- 8- dodecylene alcohol acetate according to mass ratio: Cis- 8- lanolin alcohol: 12 carbon -1- alcohol=100:6:15:7 composition configure 500 μ g altogether, rise in integrating attractant leading Effect puts up the best performance to luring for male moth, and food bait is by 24.25mL terpinyl acetate, the Tween and 25mL30 of 0.75mL DEG C warm water be made, as the synergetic effect additive of sex pheromone in mixed attractant, male moth and female moth are played closely The effect lured mixes sex pheromone solution and food bait solution according to the ratio that volume ratio is 1:0.8；It is protected as best Component is protected, medium selects the honey of 10 μ l, and antioxidant selects the Propyl Gallate(PRG of gross mass 35%), that is, it extends Duration, and increase induced moth number.
Embodiment 3
A kind of optimal integration attractant for preventing and treating oriental fruit months is made of sex pheromone, food bait and protection component, as Component is most preferably lured, sex pheromone is cis- 8- dodecylene alcohol acetate: trans- 8- dodecylene alcohol acetate according to mass ratio: Cis- 8- lanolin alcohol: 12 carbon -1- alcohol=100:7:20:2 composition configure 500 μ g altogether, rise in integrating attractant leading Effect puts up the best performance to luring for male moth, and food bait is by 24.25mL terpinyl acetate, the Span of 0.75mL and 25mL40 DEG C Warm water be made, as the synergetic effect additive of sex pheromone in mixed attractants, male moth and female moth are played and closely drawn The effect lured mixes sex pheromone solution and food bait solution according to the ratio that volume ratio is 1:1.2；As best protection Component, medium select the honey of 10 μ l, and antioxidant selects the 2,6-Di-tert-butyl-4- of gross mass 40% Methylphenol(BHT), that is, the duration is extended, and increases induced moth number.
The above content is combine specific preferred embodiment to the further description done of the present invention, and it cannot be said that A specific embodiment of the invention is only limitted to this, for those of ordinary skill in the art to which the present invention belongs, is not taking off Under the premise of from the present invention, several simple deduction or replace can also be made, all shall be regarded as belonging to the present invention by being submitted Claims determine scope of patent protection.

Claims (8)

1. attractant is integrated in a kind of field for preventing and treating oriental fruit months, which is characterized in that including sex pheromone, food bait With protection component, the sex pheromone is according to mass ratio are as follows: cis- 8- dodecylene alcohol acetate: trans- 8- lanolin alcohol vinegar Acid esters: cis- 8- lanolin alcohol: 12 carbon -1- alcohol=100:6-7:15-20:2-7 composition, the food bait includes second Sour pine tar ester, the protection group are divided into honey and antioxidant.
2. attractant is integrated in a kind of field for preventing and treating oriental fruit months according to claim 1, which is characterized in that institute The sex pheromone stated is according to mass ratio are as follows: cis- 8- dodecylene alcohol acetate: trans- 8- dodecylene alcohol acetate: cis- 8- ten Two carbene alcohol: 12 carbon -1- alcohol=100:6.8:19.1:5.4 composition.
3. attractant is integrated in a kind of field for preventing and treating oriental fruit months according to claim 1, which is characterized in that institute The volume ratio of the sex pheromone and food bait stated is 1:0.8-1.2.
4. attractant is integrated in a kind of field for preventing and treating oriental fruit months according to claim 1, which is characterized in that institute The food bait stated further includes the water of non-ionic wetting agent and 30-40 DEG C.
5. attractant is integrated in a kind of field for preventing and treating oriental fruit months according to claim 4, which is characterized in that institute The non-ionic wetting agent stated is any one in Span, Tween, APG.
6. attractant is integrated in a kind of field for preventing and treating oriental fruit months according to claim 1, which is characterized in that institute The volume for stating honey is the 0.015-0.025% of volume after sex pheromone and food bait mixing.
7. attractant is integrated in a kind of field for preventing and treating oriental fruit months according to claim 1, which is characterized in that institute The antioxidant stated is 2,6-Di-tert-butyl-4-methylphenol or Propyl Gallate.
8. attractant is integrated in a kind of field for preventing and treating oriental fruit months according to claim 1, which is characterized in that institute The quality for stating antioxidant is the 30-40% of gross mass after the sex pheromone, food bait and honey mix.
CN201910735597.7A 2019-08-09 2019-08-09 Attractant is integrated in a kind of field for preventing and treating oriental fruit months Pending CN110419532A (en)

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CN111449064A (en) * 2019-01-22 2020-07-28 杨凌职业技术学院 Novel grapholitha molesta imago sex pheromone attracting paste as well as preparation and use methods thereof
CN113057164A (en) * 2021-03-15 2021-07-02 四川物华农业科技有限公司 Grapholitha molesta attractant and attracting core
CN113068695A (en) * 2021-04-12 2021-07-06 浙江农林大学 Sex attractant for oriental fruit moth, lure and preparation method and application thereof
CN115581232A (en) * 2022-10-21 2023-01-10 延安大学 Plum fruit borer sex attractant for efficiently inhibiting oriental fruit borers and preparation method thereof
CN116076289A (en) * 2021-11-08 2023-05-09 北京市农林科学院 Method for preventing fruit tree fruit borer by combining use of vagin and phagostimulant

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孔维娜等: "三种梨小食心虫引诱剂配方的田间筛选", 《北方园艺》 *
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111449064A (en) * 2019-01-22 2020-07-28 杨凌职业技术学院 Novel grapholitha molesta imago sex pheromone attracting paste as well as preparation and use methods thereof
CN113057164A (en) * 2021-03-15 2021-07-02 四川物华农业科技有限公司 Grapholitha molesta attractant and attracting core
CN113068695A (en) * 2021-04-12 2021-07-06 浙江农林大学 Sex attractant for oriental fruit moth, lure and preparation method and application thereof
CN116076289A (en) * 2021-11-08 2023-05-09 北京市农林科学院 Method for preventing fruit tree fruit borer by combining use of vagin and phagostimulant
CN115581232A (en) * 2022-10-21 2023-01-10 延安大学 Plum fruit borer sex attractant for efficiently inhibiting oriental fruit borers and preparation method thereof
CN115581232B (en) * 2022-10-21 2024-03-01 延安大学 Plum fruit borer sex attractant for efficiently inhibiting pear fruit borer and preparation method thereof

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