CN111449064A - Novel grapholitha molesta imago sex pheromone attracting paste as well as preparation and use methods thereof - Google Patents
Novel grapholitha molesta imago sex pheromone attracting paste as well as preparation and use methods thereof Download PDFInfo
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- CN111449064A CN111449064A CN201910058730.XA CN201910058730A CN111449064A CN 111449064 A CN111449064 A CN 111449064A CN 201910058730 A CN201910058730 A CN 201910058730A CN 111449064 A CN111449064 A CN 111449064A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
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- Agronomy & Crop Science (AREA)
- Insects & Arthropods (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a novel grapholitha molesta imago sex pheromone attracting paste as well as a preparation method and a use method thereof, wherein the paste comprises the following components: the ointment comprises cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol, an auxiliary agent, a matrix and the like, wherein the mass ratio of the cis-8-dodecene acetate to the trans-8-dodecene acetate to the cis-8-dodecenol to the auxiliary agent to the matrix is 0-10: 0-1: 0-0.1: 0-1: 0-100, and the field lasting period of the ointment prepared by the method is not less than 4 weeks. The paste attractant prepared by the method is matched with a special trap for use, can achieve the same attracting effect as the traditional attractant paste, and is convenient to use; can be used for replacing the prior grapholitha molesta sex pheromone lure core made of rubber plugs and plastic capillary tubes.
Description
Technical Field
The invention relates to the technical field of fruit tree pest control, and particularly relates to a novel grapholitha molesta sex pheromone attractant paste, a preparation method and a use method.
Background
Grapholitha molesta (Busck), an insect of the genus Grapholitha of the family Tolydidae, is a species. The fruit tree pests in the world have the advantages that the larvae mostly eat fruits from calyx and stem depressions, insect and excrement is discharged outside the wormholes of the damaged fruits at the early stage, and the insects and excrement are mostly avoided at the later stage. The young insects can directly enter the fruit core, and the periphery of the moth holes usually becomes black and gradually becomes wider under the high humidity condition, so the method is commonly called as 'black plaster'. The larva harms the young shoot and usually moths from the base part of the upper petiole to the medulla part and then transfers to lignification, the moth hole is gummy and has insect feces, and the damaged young shoot is gradually withered and is commonly called as the broken tip.
The oriental fruit moth tries one of the important pests of northern fruit trees, the host of the oriental fruit moth comprises various kernel fruits and stone fruit trees such as pears, peaches, apples, nectarines, cherries and the like, the oriental fruit moth has a plurality of generations in one year, the generations are seriously overlapped, the boring property of the larva and the diversity of the host bring great difficulty to prevention and control, the large-area occurrence of the oriental fruit moth is caused by the adjustment of the industrial structure and the increase of the planting area of the fruit trees, the rising trend is generated, and the quality and the yield of the fruit trees are seriously influenced.
At present, the prevention and control of the oriental fruit moth are still mainly achieved, but biological prevention and control are widely popularized in recent years, for example, the oriental fruit moth sex pheromone is used for insect condition detection and prediction, a large number of traps and interferences are mated, however, accurate insect state detection needs to be carried out on the oriental fruit moth in any prevention and control mode, the oriental fruit moth attractant is widely applied to scientific research and market popularization as a mature technology, and insect pest occurrence rules can be accurately mastered. The main models of the mature sex pheromone attracting products are divided into two types: one is rubber as carrier, and the other is polyvinyl chloride capillary as carrier.
The insect sex pheromone is a trace chemical substance which is secreted in vitro by an individual of a certain sex of an insect, is accepted by a receptor of an individual of the same sex, and causes the individual of the opposite sex to generate a certain reproductive behavior response (such as foraging orientation, mating and the like). The application of insect sex pheromones and traps to monitor and control pests has been developed into an effective green prevention and control means and has been widely applied to many pests, especially lepidopteran pests. Has the advantages of high sensitivity, high accuracy, simplicity, low cost and the like, and is being widely applied.
The invention provides CN201310699921.7 a oriental fruit moth imago attractant, an attractant core and a preparation method thereof, and discloses the oriental fruit moth imago attractant which comprises cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecene-1-ol and trans, trans-8, 10-dodecene-1-ol. On the basis, the invention also discloses a lure based on the attractant and a preparation method thereof. The attractant disclosed by the invention has a remarkable attracting effect on the oriental fruit moth imagoes, and can be used for monitoring the occurrence dynamics of the oriental fruit moth in the field and guiding chemical control, or directly controlling the oriental fruit moth by a mass attracting and killing method.
The attractant and lure referred to in the above patent; the lure core is selected from a rubber plug, a capillary tube and a plastic centrifuge tube; most of the current attractants are matched with a lure core for use, and the lure core is arranged in the trap.
The use method of the existing attractant core-attracting rubber plugs is that after the thin-wall part (about 1 mm-1.5 mm) is pricked by an iron wire, the rubber plugs are hung in the trapper, or the attractant cores of the rubber plugs are directly clamped into the attractant core clamping grooves of the trapper; the capillary lure is used in a manner similar to a rubber stopper lure and is inserted into the lure insertion hole of the trap. Therefore, the two types of lure cores are in direct contact with operators when in use, the lure cores of the lure agents are very easy to be polluted in the process due to the sensitivity of the pheromone lure agent products, so that the lure cores have no lure effect or poor lure effect, in addition, the hole and groove modes adopted by fixation also have the defect that the lure cores fall off in the use process, and the risk of stabbing users is caused in the process that the iron wires penetrate through the rubber plugs.
The paste attractant disclosed by the invention has the advantages that a user does not directly contact with the attractant in the using process, so that the attractant is prevented from being polluted, the paste is directly smeared in the trap by the user, and the paste can be adhered in the trap due to certain viscosity, so that the operation is simple and no use risk exists.
Disclosure of Invention
The technical problem to be solved by the invention is to select a proper paste matrix and the proportion of each component to realize the slow release of the attractant, realize the trapping of the Grapholitha molesta adults by using a matched trap, and avoid the artificial contact to reduce the trapping effect.
In order to achieve the purpose, the invention adopts the technical scheme that:
a novel sex pheromone attracting paste for adult Grapholitha molesta Busck,
the ointment consists of cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol, an auxiliary agent and a matrix, wherein the mass ratio of the cis-8-dodecene acetate to the trans-8-dodecene acetate to the cis-8-dodecenol to the auxiliary agent is 0-10: 0-1: 0-0.1: 0-1: 0-100.
The novel grapholitha molesta imago sex pheromone attracting paste comprises the following active ingredients: cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol;
the weight ratio of the active ingredients of the sex pheromone is 80-110: 5-10: 1 to 2.
The novel grapholitha molesta imago sex pheromone attracting paste comprises the active ingredients, wherein the weight ratio of the sex pheromone active ingredients is 86:6: 2.
The novel grapholitha molesta imago sex pheromone attracting paste comprises the active ingredients, wherein the weight ratio of the sex pheromone active ingredients is 85:8: 2.
The novel grapholitha molesta imago sex pheromone attracting paste is characterized in that the weight ratio of the sex pheromone effective components is 102:9: 1.
The novel grapholitha molesta imago sex pheromone attractant paste comprises an antioxidant, an anti-ultraviolet agent and a solvent;
the matrix comprises adhesive and inorganic filler, wherein the inorganic filler is 200-400 meshes.
A preparation method of a novel grapholitha molesta imago sex pheromone attracting paste,
1) firstly, cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol and an auxiliary agent are mixed according to the mass ratio of 5: 0.5: fully and uniformly mixing the components in a ratio of 0.01: 0.4;
2) adding the uniformly mixed liquid mixture in the step 1) into 200-mesh inorganic filler, wherein the mass ratio of the liquid mixture to the inorganic filler is 1: 500; fully stirring the mixture to ensure that the liquid is uniformly adhered to the inorganic filler; adding adhesive with equal mass ratio, and stirring in a constant temperature oil bath at 40 deg.C to make inorganic filler particles carrying effective components of grapholitha molesta sex pheromone contact with the adhesive and wrap the adhesive; thus obtaining the paste finished product.
The novel grapholitha molesta imago sex pheromone attracting paste is prepared by adding ethanol when the resistance is large during stirring, and uniformly stirring and mixing to obtain a paste finished product.
A novel usage method of sex pheromone attracting paste for adult oriental fruit moth is characterized in that the paste is smeared on the inner side of a trapping device when in use, the smearing amount is about 1 g each time, and the lasting period is more than 4 weeks under normal weather conditions.
Compared with the prior art, the invention has the following beneficial effects:
the fluke sex pheromone is used for preparing the slow-release paste to be matched with the special trap for use, and the slow-release paste can be directly smeared in the trap for use, so that green monitoring, prevention and control of fluke adults in an orchard can be successfully realized;
the invention selects the sex pheromone of the oriental fruit moth as the main effective component of the attractant, uses the viscose and the inorganic filler as the substrate, and is matched with the auxiliary agent to prepare a novel formulation of the sex pheromone attractant of the oriental fruit moth with strong attraction effect, namely paste under a certain processing method;
the paste attractant disclosed by the invention has the advantages that a user does not directly contact with the attractant in the using process, so that the attractant is prevented from being polluted, the paste is directly smeared in the trap by the user, and the paste can be adhered in the trap due to certain viscosity, so that the operation is simple and no use risk exists.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The novel grapholitha molesta adult sex pheromone attracting paste consists of cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol, an auxiliary agent and a matrix, and the mass ratio of the cis-8-dodecene acetate to the trans-8-dodecene acetate to the cis-8-dodecenol to the auxiliary agent is 0-10: 0-1: 0-0.1: 0-1: 0-100.
The invention relates to a novel grapholitha molesta adult sex pheromone attracting paste, which comprises the following effective components: cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol; the weight ratio of the active ingredients of the sex pheromone is 80-110: 5-10: 1-2, wherein three preferred embodiments are 86:6:2, 85:8:2 and 102:9:1 respectively.
The novel grapholitha molesta adult sex pheromone attracting paste comprises an antioxidant, an anti-ultraviolet agent and a solvent; the antioxidant is tea polyphenol, tocopherol, dibutyl hydroxy toluene (BHT) or tert-butyl hydroquinone (TBHQ); the anti-ultraviolet agent is phenyl o-hydroxybenzoate or 2, 4-dihydroxy benzophenone; the solvent is ethanol or glycerol.
The matrix comprises adhesive and inorganic filler, wherein the inorganic filler is 200-400 meshes;
wherein the adhesive is one or more of polyisobutylene adhesive, silicone rubber adhesive, polyacrylate adhesive, polyethylene hot melt adhesive and polypropylene hot melt adhesive.
The inorganic filler is one or more of light calcium carbonate, heavy calcium carbonate, kaolin, diatomite and talcum powder.
The invention relates to a preparation method of a novel grapholitha molesta imago sex pheromone attracting paste,
1) firstly, cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol and an auxiliary agent are mixed according to the mass ratio of 5: 0.5: fully and uniformly mixing the components in a ratio of 0.01: 0.4;
2) adding the uniformly mixed liquid mixture in the step 1) into 200-mesh inorganic filler, wherein the mass ratio of the liquid mixture to the inorganic filler is 1: 500; fully stirring the mixture to ensure that the liquid is uniformly adhered to the inorganic filler; adding adhesive with equal mass ratio, and stirring in a constant temperature oil bath at 40 deg.C to make inorganic filler particles carrying effective components of grapholitha molesta sex pheromone contact with the adhesive and wrap the adhesive; thus obtaining the paste finished product.
The novel grapholitha molesta adult sex pheromone attracting paste is prepared by adding ethanol into the paste when the resistance is high during stirring, and uniformly stirring and mixing the mixture to obtain a paste finished product.
The application method of the novel grapholitha molesta adult sex pheromone attracting paste is characterized in that the paste is smeared on the inner side of a pest attracting device when in use, the smearing amount is about 1 g each time, and the lasting period under normal weather conditions is more than 4 weeks.
The present invention is described in detail below with reference to examples:
example 1:
the weight ratio of cis-8-dodecene acetate, trans-8-dodecene acetate and cis-8-dodecenol is 86:6:2, and the cis-8-dodecene acetate, the antioxidant and the ultraviolet resistant agent are mixed according to the mass ratio of 5: 0.5: fully and uniformly mixing the components in a ratio of 0.01: 0.4; then adding inorganic filler with the mass ratio of 1: 500; fully stirring the mixture to ensure that the liquid is uniformly adhered to the inorganic filler; adding adhesive with equal mass ratio, and stirring in a constant temperature oil bath at 40 deg.C to make inorganic filler particles carrying effective components of grapholitha molesta sex pheromone contact with the adhesive and wrap the adhesive; obtaining a paste finished product; if the stirring resistance is large, a proper amount of ethanol can be added.
Example 2:
otherwise, as in example 1, the pheromone active ingredient was changed to cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol in a weight ratio of 85:8: 2; preparing a pasty finished product;
example 3:
otherwise, as in example 1, the pheromone active ingredient was changed to cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol in a weight ratio of 102:9: 1; preparing a pasty finished product;
the ingredient ratios of the examples are shown in the following table:
the experiment period is from 6 months and 5 days in 2018 to 7 months and 1 day in 2018, and the experiment place is as follows: test ground of the Shanxi Yangling institute of occupational technology.
The test area was divided into 3 test areas, each area having a size of about 2 acres and each area was provided with the trap of each of the example 1 sets, and the trap data was investigated once per week.
The experimental results are tabulated below:
the field trapping experiment shows that the grapholitha molesta imago sex pheromone attractant has a strengthened attraction effect on the grapholitha molesta imago, and simultaneously shows that different slow-release carrier ratios can cause remarkable influence on the attraction effect.
In conclusion, the novel attractant for attracting the imagoes of the oriental fruit moth provided by the invention is obviously different from the existing commercial attractants, can simply, conveniently, environmentally and efficiently realize the monitoring and control of the imagoes of the oriental fruit moth, and has remarkable social and ecological benefits.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. Not all embodiments are exhaustive. All obvious changes and modifications which are obvious to the technical scheme of the invention are covered by the protection scope of the invention.
Claims (9)
1. A novel grapholitha molesta imago sex pheromone luring ointment is characterized in that: the ointment consists of cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol, an auxiliary agent and a matrix, wherein the mass ratio of the cis-8-dodecene acetate to the trans-8-dodecene acetate to the cis-8-dodecenol to the auxiliary agent is 0-10: 0-1: 0-0.1: 0-1: 0-100.
2. The novel grapholitha molesta imago sex pheromone attractant paste as claimed in claim 1, wherein:
the sex pheromone of the oriental fruit moth comprises the following effective components: cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol;
the weight ratio of the active ingredients of the sex pheromone is 80-110: 5-10: 1 to 2.
3. The novel grapholitha molesta imago sex pheromone attractant paste as claimed in claim 2, wherein:
the weight ratio of the active ingredients of the sex pheromone is 86:6: 2.
4. The novel grapholitha molesta imago sex pheromone attractant paste as claimed in claim 2, wherein:
the weight ratio of the active ingredients of the sex pheromone is 85:8: 2.
5. The novel grapholitha molesta imago sex pheromone attractant paste as claimed in claim 2, wherein:
the weight ratio of the active ingredients of the sex pheromone is 102:9: 1.
6. The novel grapholitha molesta adult sex pheromone attracting paste according to any one of claims 1 to 5, wherein:
the auxiliary agent comprises an antioxidant, an anti-ultraviolet agent and a solvent;
the matrix comprises adhesive and inorganic filler, wherein the inorganic filler is 200-400 meshes.
7. A preparation method of a novel grapholitha molesta adult sex pheromone attracting paste is characterized by comprising the following steps:
1) firstly, cis-8-dodecene acetate, trans-8-dodecene acetate, cis-8-dodecenol and an auxiliary agent are mixed according to the mass ratio of 5: 0.5: fully and uniformly mixing the components in a ratio of 0.01: 0.4;
2) adding the uniformly mixed liquid mixture in the step 1) into 200-mesh inorganic filler, wherein the mass ratio of the liquid mixture to the inorganic filler is 1: 500; fully stirring the mixture to ensure that the liquid is uniformly adhered to the inorganic filler; adding adhesive with equal mass ratio, and stirring in a constant temperature oil bath at 40 deg.C to make inorganic filler particles carrying effective components of grapholitha molesta sex pheromone contact with the adhesive and wrap the adhesive; thus obtaining the paste finished product.
8. The preparation method of the novel grapholitha molesta imago sex pheromone attractant paste according to claim 7, wherein the preparation method comprises the following steps:
when the resistance is large during stirring, ethanol can be added, and the paste finished product is obtained after uniform stirring and mixing.
9. A use method of a novel grapholitha molesta adult sex pheromone attracting paste is characterized by comprising the following steps:
when in use, the paste is smeared on the inner side of the insect trapping device, the smearing amount is about 1 gram each time, and the lasting period is more than 4 weeks under normal weather conditions.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113057164A (en) * | 2021-03-15 | 2021-07-02 | 四川物华农业科技有限公司 | Grapholitha molesta attractant and attracting core |
| CN113068695A (en) * | 2021-04-12 | 2021-07-06 | 浙江农林大学 | Sex attractant for oriental fruit moth, lure and preparation method and application thereof |
| CN113498780A (en) * | 2021-06-21 | 2021-10-15 | 河北省农林科学院昌黎果树研究所 | Paste for efficiently inducing and controlling oriental fruit moth and preparation method thereof |
| CN114041474A (en) * | 2021-12-03 | 2022-02-15 | 山东省农药科学研究院 | Distributor of insect pheromone lure core and manufacturing method and application thereof |
| CN115119843A (en) * | 2022-06-08 | 2022-09-30 | 中捷四方生物科技股份有限公司 | Grapholitha molesta Busck attractant |
| CN115581232A (en) * | 2022-10-21 | 2023-01-10 | 延安大学 | Plum fruit borer sex attractant for efficiently inhibiting oriental fruit borers and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113057164A (en) * | 2021-03-15 | 2021-07-02 | 四川物华农业科技有限公司 | Grapholitha molesta attractant and attracting core |
| CN113068695A (en) * | 2021-04-12 | 2021-07-06 | 浙江农林大学 | Sex attractant for oriental fruit moth, lure and preparation method and application thereof |
| CN113498780A (en) * | 2021-06-21 | 2021-10-15 | 河北省农林科学院昌黎果树研究所 | Paste for efficiently inducing and controlling oriental fruit moth and preparation method thereof |
| CN114041474A (en) * | 2021-12-03 | 2022-02-15 | 山东省农药科学研究院 | Distributor of insect pheromone lure core and manufacturing method and application thereof |
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| CN115581232A (en) * | 2022-10-21 | 2023-01-10 | 延安大学 | Plum fruit borer sex attractant for efficiently inhibiting oriental fruit borers and preparation method thereof |
| CN115581232B (en) * | 2022-10-21 | 2024-03-01 | 延安大学 | Plum fruit borer sex attractant for efficiently inhibiting pear fruit borer and preparation method thereof |
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