CN110292043B

CN110292043B - Sex attractant for tuber moth with optimized system and application thereof

Info

Publication number: CN110292043B
Application number: CN201910710064.3A
Authority: CN
Inventors: 高玉林; 闫俊杰; 梅向东; 王留洋; 宁君
Original assignee: Institute of Plant Protection of Chinese Academy of Agricultural Sciences
Current assignee: Institute of Plant Protection of Chinese Academy of Agricultural Sciences
Priority date: 2019-08-02
Filing date: 2019-08-02
Publication date: 2020-09-04
Anticipated expiration: 2039-08-02
Also published as: CN110292043A

Abstract

The invention discloses a sex attractant for tuber moths optimized by a system and application thereof, belonging to the technical field of insect sex pheromone preparation. The sex attractant for the tuber moths comprises sex pheromones of the tuber moths and gland extracts of the female tuber moths, wherein the sex pheromones of the tuber moths are trans-4, cis-7-tridecadiene-1-ol acetate and trans-4, cis-7, cis-10-tridecatrien-1-ol acetate, and the ratio of the sex pheromones to the trans-4, cis-7, cis-10-tridecatrien-1-ol acetate is 1: 4. The female gland extract of the tuber moth is palmitic acid, and is added into the tuber moth pheromone in a proportion of 0.01-10%. The sex attractant for the tuber moths can effectively attract males, the sex attractant for the tuber moths added in a proportion of 10% is increased by 58% compared with the sex pheromone, and the lasting period can be prolonged to 60 days, so that the meeting probability of male and female insects can be reduced for a long time, the mating is interfered, the reproduction rate is reduced, the pest population is gradually controlled, and the purpose of preventing and controlling pests is achieved.

Description

Sex attractant for tuber moth with optimized system and application thereof

Technical Field

The invention belongs to the technical field of insect sex pheromone preparation, and particularly relates to a sex attractant for a tuber moth with an optimized system and application thereof.

Background

The tuber moth of potato (also called tobacco leaf miner: Gelechiideae) is a worldwide agricultural pest harmful to solanaceous crops, especially to potato, and has destructive damage to potato, as its main host potato is originally produced in northern regions of Central and south America, and is now distributed in more than 100 countries of Asia, Europe, North America, Africa, etc. While the damage to potato flakes generally does not seriously affect yield, the damage to potato pieces can significantly reduce yield, especially up to 100% without low temperature storage. At present, the insects are distributed in more than 20 provinces and regions in China, such as Yunnan, Guizhou, Sichuan, Guangdong, Guangxi, Hunan, Jiangxi, Gansu and the like, and the damage of the tuber moths of the potatoes tends to be more serious year by year along with the increase of the planting area of the potatoes and the frequent transportation of the seed potatoes. The long-term use of chemical agents for controlling the tuber moths can cause the pests to generate drug resistance, kill natural enemies, pollute the environment, destroy the ecological system and the like. In the face of various problems brought by the application of chemical agents, human beings gradually turn into green prevention and treatment and explore a sustainable control mode. According to the principle that sex pheromone released by female insects has strong attraction capacity to the same kind of male insects, the sex pheromone components and the proportion of female tuber moths are determined, and the sex attractant formula is continuously optimized, so that the sex pheromone can be accurately applied to insect condition observation and prediction, pest quarantine, mating interference and mass trapping, and the ideal effect of comprehensively treating the tuber moths is achieved.

Disclosure of Invention

The invention aims to provide a sex attractant for tuber moths with optimized systems and application thereof, which are used for insect condition prediction, pest quarantine, mating interference and mass trapping of the tuber moths and achieve the ideal effect of comprehensively treating the tuber moths.

The invention provides a sex attractant for a tuber moth, which is optimized by a system and comprises a sex pheromone of the tuber moth and a gland extract of a female insect of the tuber moth.

Preferably, the tuber moth sex pheromone comprises trans-4, cis-7-tridecadiene-1-ol acetate and trans-4, cis-7, cis-10-tridecatrien-1-ol acetate in a ratio of 1: 4.

Preferably, the female gland extract of the tuber moth is hexadecanoic acid, and the addition amount of the female gland extract is 0.01% -10% of the sex pheromone of the tuber moth.

Preferably, the female gland extract of the tuber moth is hexadecanoic acid, and the addition amount of the female gland extract is 10% of sex pheromone of the tuber moth.

More preferably, the sex pheromone of the potato tuber moth comprises trans-4, cis-7-tridecadiene-1-ol acetate and trans-4, cis-7, cis-10-tridecatrien-1-ol acetate, the proportion of the trans-4, cis-7, cis-10-tridecatrien-1-ol acetate is 1:4, the female gland extract of the potato tuber moth is hexadecanoic acid, and the addition amount of the female gland extract is 10% of the sex pheromone of the potato tuber moth.

The invention also provides a using method of the sex attractant for the potato tuber moth with the optimized system, which comprises the steps of adding 100-1000 mu g of the sex attractant into 1mL of volatile solvent n-hexane, uniformly mixing, dropwise adding the mixture into the butyl rubber head lure core, and placing the butyl rubber head lure core in the center of the triangular trapper after the n-hexane is volatilized to obtain the sex attractant for the potato tuber moth.

The invention also provides application of the sex attractant for the tuber moth, which is optimized by the system and is used for quarantine of the insect pests of the tuber moth, insect condition monitoring, mass trapping and mating interference.

Has the advantages that: the sex attractant for the tuber moths can effectively attract the tuber moths, and is simple to prepare and feasible in conditions. The method has strong trapping capability and good stability, can be accurately applied to insect condition observation and prediction, pest quarantine, mating interference and mass trapping, and achieves the ideal effect of comprehensively treating the potato tuber moth; the specificity is strong, resistance of the potato tuber moth can not be generated, and the potato tuber moth has no harm to natural enemies; is nontoxic to human and livestock and is environment-friendly; the stress resistance is strong, and the wind, rain and sunshine resistance is realized; the duration is long, and the control effect can be maintained for at least 60 days.

Drawings

FIG. 1 is a comparison of the duration of action of sex attractants and sex pheromones against tuber moth.

Detailed Description

The present invention is described in detail below with reference to the attached drawings and the specific embodiments, but it should be understood that the scope of the present invention is not limited by the specific embodiments.

Example 1 preparation of sex attractant for Tuber moth

The specific dosage of each component of the sex attractant for the potato tuber moth is determined according to the following weight percentage ranges: trans-4, cis-7-tridecadiene-1-ol acetic acid and trans-4, cis-7, cis-10-tridecatrien-1-ol acetate in a ratio of 1: 4; adding 0.01-10% of hexadecanoic acid into sex pheromone of the tuber moth of potatoes, wherein the amount of n-hexane is 1mL, mixing the sex attractant of the tuber moth of potatoes with a solvent (the n-hexane), adding the mixed solution into a lure core of a butyl rubber head, placing the lure core into a triangular trapper after the solvent is completely volatilized, and then placing the trapper in a region where the tuber moth of potatoes is serious. In order to ensure the release effect, 100-1000 mug of potato tuber moth sex attractant is put into each 1mL of rubber lure, and the average distance between traps is more than 15 m. To further illustrate the feasibility of this example, 6 specific formulations are given in table 1:

TABLE 1 sex attractant for tuber moth of potato

The experiment is carried out in the city of Qujing, Yunnan, Malong region, Yuwangxi Hai village. A connected potato field with the potato variety, the growth condition and the fertilizer and water management condition consistent, the area of the field being more than 30 mu and the potato tuber moth occurring seriously is selected as a test field, 5 repeated triangular traps containing sex attractants are set up for each treatment, 5 blank triangular traps and 5 triangular traps containing 100 mu g of potato tuber moth sex pheromone are randomly placed, and the triangular traps are randomly arranged. The average spacing between traps is above 15 m. After the placement, the number of the induced male potato tuber moths is investigated every three days, the trapping positions are randomly changed, the investigation is continued for 30 days, and the test effect is shown in table 2:

TABLE 2 moth-luring effect of different formulations of sex attractant for potato tuber moth

Note: data in the table are mean. + -. standard deviation

The test result shows that the total trapping amount and the average trapping amount of the 6 groups of formulas of the sex attractant for the tuber moths optimized by the system are both obviously higher than that of the sex pheromones of the tuber moths, wherein the effect of the formula 6 (80 mu g of trans-4, cis-7-tridecadiene-1-ol acetate, 20 mu g of trans-4, cis-7, cis-10-tridecatrien-1-ol acetate and 10 mu g of hexadecanoic acid) is the best, and the trapping amount is improved by 58 percent compared with that of the simple pheromones.

Example 2 duration of sex attractant for Tuber moth

The investigation was continued for 5 replicates of the optimal formulation 6 and sex pheromone in example 1, in a manner consistent with example 1. The experimental results are shown in fig. 1, and the experimental results show that the sex attractant for the systemic optimized potato tuber moth can continuously trap male potato tuber moth in 75 days except in rainy days (No. 5/23 and No. 6/5), and the moth-trapping effect can be stably exerted in 60 days.

Therefore, the sex attractant for the potato tuber moth, which is optimized by the system, can be accurately applied to trapping and disturbing mating and insect pest situation prediction of a large number of potato tuber moths, and has important significance for green control of the potato tuber moths.

The above disclosure is only one specific embodiment of the present invention, but the present invention is not limited thereto, and other embodiments of the present invention may be adopted, and any changes that can be made by those skilled in the art should fall within the scope of the present invention.

Claims

1. The sex attractant for the tuber moths is characterized by comprising tuber moth sex pheromones and a tuber moth female gland extract, wherein the tuber moth sex pheromones comprise trans-4, cis-7-tridecadiene-1-ol acetate, trans-4, cis-7, cis-10-tridecatrien-1-ol acetate, the mass ratio of the trans-4 to the cis-7 to the cis-10-tridecatrien-1-ol acetate is 1:4, the tuber moth female gland extract is hexadecanoic acid, and the addition amount of the hexadecanoic acid is 10% of the mass of the tuber moth sex pheromones.

2. The use method of the sex attractant for the tuber moths optimized by the system as claimed in claim 1 is characterized in that 100-1000 μ g of the sex attractant is added into 1mL of volatile solvent n-hexane, the mixture is uniformly mixed and then is dripped into the butyl rubber head lure core, the butyl rubber head lure core is prepared after the n-hexane is volatilized, and the butyl rubber head lure core is placed in the center of a triangular trapper and used for trapping the tuber moths.

3. Use of a sex attractant for tuber moth optimized according to the system of claim 1 for pest quarantine, pest situation monitoring, mass trapping and mating disruption of tuber moth.