CN113068694A - Attractant composition for preventing and treating mythimna laurenci and application thereof - Google Patents
Attractant composition for preventing and treating mythimna laurenci and application thereof Download PDFInfo
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- CN113068694A CN113068694A CN202110343272.1A CN202110343272A CN113068694A CN 113068694 A CN113068694 A CN 113068694A CN 202110343272 A CN202110343272 A CN 202110343272A CN 113068694 A CN113068694 A CN 113068694A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses an attractant composition for preventing and treating Cladosporium laurentii and application thereof. The attractant composition of the present invention includes a mythimna laevis sex pheromone and a plant volatile component. The Rous armyworm sex pheromones include cis-9-tetradecene acetate and cis-7-dodecene acetate. The plant volatile matter is one or more of 6-methyl-5-heptene-2-ketone, 1-octene-3-alcohol and phenethyl acetate. The ratio of the Lawsonia inermis sex pheromone to the plant volatile matter is 1: 0.05-0.2. The attractant composition has strong trapping capability on the armyworm, is environment-friendly, has no toxicity or harm to a human body, and has cheap and easily-obtained raw materials. The attractant composition disclosed by the invention can be accurately applied to insect condition prediction and mass trapping, and has important significance for comprehensively preventing and controlling the mythimna laurenci.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to an attractant composition for controlling Cladosporium laurentii and application thereof.
Background
The Lao's armyworm Leucania loreyi (Duponcel) belongs to Lepidoptera (Lepidotera) Noctuidae (Noctuidae), and is mainly distributed in Guangdong, Gansu, Fujian, Sichuan, Jiangxi, Hunan, Hubei, Zhejiang, Jiangsu, Shandong, Henan, Hebei and the like in China. The larvae are very impure in feeding property, mainly harm gramineous crops such as rice, corn, sorghum, wheat and the like, and grow fast by feeding the corn and the wheat, and are one of important pests on the corn at present. Research shows that the occurrence rules of the Lao's armyworm are slightly different in different geographical positions, the Lao's armyworm can occur for 6-7 generations in Guangdong one year, and the theory speculates that the Lao's armyworm can occur for 5-7 generations in Guangxi one year and the Henan and Tuping can occur for 3-4 generations, but the Lao's armyworm cannot overwinter in Henan and Hebei, and the root cause influencing the growth and development of the Lao's armyworm is the climate temperature. At present, the research on the Lawsonia inermis is less in China, and the research mainly focuses on the research on the problems of initial temperature, effective accumulated temperature, short-time high temperature, feeding property of the Lawsonia inermis and the like. The armyworm is often mixed with spodoptera frugiperda, cotton bollworm and oriental armyworm on corn, and poses serious threat to crops such as corn.
In the comprehensive treatment of plant diseases and insect pests, chemical prevention and control are always one of the main means. However, the prevention and control effect is obviously reduced along with the generation of the drug resistance of pests, and meanwhile, the application of chemical pesticides can cause a series of problems of influencing the survival of natural enemies, causing ecological environment damage, ensuring food quality safety and the like, thereby becoming an important obstacle for the sustainable development of agriculture. Research reports that with the wide use of chemical insecticides, the tolerance of most lepidoptera pests to chemical insecticides such as organophosphates, carbamates and pyrethroids is obviously enhanced, and the phenomenon of resistance to the insecticides is increasingly prominent, so that the development of green control is urgent. The insect sex pheromone has the advantages of high efficiency, low toxicity, no pollution, no damage to beneficial insects and the like, and plays an increasingly important role in Integrated Pest Management (IPM). The plant-feeding imagoes generally seek a proper host through plant volatile smell to seek a series of physiological activities such as puppet, mating and food taking, and the like, and the chemical communication system is regulated and controlled by utilizing insect sex pheromones and the plant volatile smell, so that the plant-feeding imagoes have important significance for efficiently preventing and controlling target pests.
Disclosure of Invention
The invention aims to provide an attractant composition for preventing and controlling mythimna laurenci.
A attractant composition for preventing and treating Lawsonia inermis comprises Lawsonia inermis sex pheromone and plant volatile components.
A attractant composition for preventing and treating Lawsonia armyworm, wherein the Lawsonia armyworm sex pheromone components are cis-9-tetradecene acetate and cis-7-dodecene acetate, and the plant volatile matter is one or more of 6-methyl-5-heptene-2-one, 1-octene-3-alcohol and phenethylacetate.
Optionally, the mass ratio of the Lawsonia inermis sex pheromone to the plant volatile matter components is 1: 0.05-0.2; the plant volatile matter comprises the following components in percentage by mass: 10-35% of 6-methyl-5-heptylene-2-ketone, 0-25% of 1-octene-3-ol and 45-90% of phenethyl acetate.
Preferably, the Clavicia laurentii sex pheromone has a mass of 1000 μ g, including 900 μ g of cis-9-tetradecene acetate and 100 μ g of cis-7-dodecene acetate. The plant volatile matter is 50-200 mu g in mass and comprises the following components in percentage by mass: 10-25% of 6-methyl-5-heptylene-2-ketone, 0-25% of 1-octene-3-ol and 45-80% of phenethyl acetate.
Preferably, the Clavicia laurentii sex pheromone has a mass of 1000 μ g, including 900 μ g of cis-9-tetradecene acetate and 100 μ g of cis-7-dodecene acetate. The plant volatile matter is 50-200 mu g in mass and comprises the following components in percentage by mass: 25% of 6-methyl-5-hepten-2-one, 20% of 1-octen-3-ol and 55% of phenethyl acetate.
More preferably, the Clavicia laurentii sex pheromone has a mass of 1000 μ g, including 900 μ g of cis-9-tetradecene acetate and 100 μ g of cis-7-dodecene acetate. The plant volatile matter is 160 mug in mass and comprises the following components in percentage by mass: 25% of 6-methyl-5-hepten-2-one, 20% of 1-octen-3-ol and 55% of phenethyl acetate.
The invention also provides a lure core for preventing and controlling the mythimna separata, which comprises the lure agent composition of the mythimna separata.
A lure core for preventing and controlling Lawsonia inermis is prepared by the following steps:
(1) selecting a lure type of a polyvinyl chloride capillary tube (with the length of 10mm and the inner diameter of 1.5mm) or a polyethylene small bottle (with the height of 2-2.5 cm, the diameter of 0.5-0.6 cm and the thickness of 0.02-0.03 cm);
(2) diluting the Lawsonia inermis sex pheromone and plant volatile matter of the above claims with chlorinated paraffin or refined corn oil to gradient concentration, sucking a certain volume (10-150 μ L) according to dosage, and adding into the lure.
(3) And (4) placing the prepared lure in a refrigerator at the temperature of-20 ℃ for storage for later use.
The invention also provides application of the attractant composition in the aspect of preventing and controlling the mythimna laurenci.
The lure was placed above a bucket-type trap with a height of 1.0-1.5m selected and an average spacing of 20-30 m.
Compared with the prior art, the invention has the following beneficial effects: the attractant composition has strong trapping capability on the Cladosporium laurentii, is environment-friendly, has no toxicity or harm to human bodies, is cheap and easily available in raw materials, is simple to operate, can be accurately applied to insect condition prediction and mass trapping, and has important significance for comprehensively preventing and controlling the Cladosporium laurentii.
Drawings
FIG. 1 is a schematic diagram of the chemical structure of 6-methyl-5-hepten-2-one.
FIG. 2 is a schematic diagram of the chemical structure of 1-octen-3-ol.
FIG. 3 is a schematic diagram of the structure of phenylethyl acetate.
Detailed Description
The following detailed description of the present invention is provided in conjunction with the accompanying drawings, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Example 1 field trap test of Clavicia laurentii sex attractant composition
Preparing a lure:
selecting a lure type of polyvinyl chloride capillary (with the length of 10mm and the inner diameter of 1.5mm) or a polyethylene small bottle (with the height of 2-2.5 cm, the diameter of 0.5-0.6 cm and the thickness of 0.02-0.03 cm), and selecting refined corn oil as a dispersion medium. In order to reduce the influence of impurities possibly existing in the lure on the field trapping effect, a 95% alcohol solution is added to soak polyvinyl chloride capillary tubes or polyethylene vials for more than 8 hours before the Lawsonia inermis sex pheromone and plant volatile matters are added, and then the polyvinyl chloride capillary tubes or the polyethylene vials are placed at a ventilation opening for air drying. Corn oil is used for diluting the Lawsonia inermis sex pheromone and the plant volatile matter into gradient concentration, and the gradient concentration is added into blank lure cores according to the formula proportion, in order to ensure the realization effect, the component dosage in each lure core is between 30 and 1500 mu g, and the volume is between 10 and 150 mu L. After the compound is completely added into the lure, the opening of the opening is sealed by an electric iron, and the opening is placed in a refrigerator and refrigerated for standby.
Selecting a corn field with an area of more than 50 mu and serious damage of the Lawsonia armyworm as a test plot. The trapper is a barrel-shaped trapper, the height of the lure cores from the ground is 1.2-1.5m, and the distance between the lure cores is 15-20 m. Table 1 gives 20 specific formulations, each with 6 replicates, and a blank lure (CK) treatment. The treatment numbers for the different formulations are shown in table 1.
TABLE 1
The number of traps was investigated every 3 days and the lure sites were randomly changed for a continuous survey of 30 days. The results of the examination are shown in Table 2.
TABLE 2
Different lower case letters indicate significant differences at the level of P < 0.05 in the Tukey's method significance test.
As can be seen from the results in Table 2, the attractant composition of the present invention has a good effect of trapping Clamyxoma laurenci. In the Rous armyworm sex pheromone, a single-component or double-component plant volatile matter in the field can improve the trapping quantity of the Rous armyworm to a certain extent, but the treatment has no significant difference. The addition of three plant volatile matters has a remarkable synergistic effect on the myxobolus reinitiae sex pheromone, and the average trapping quantity has a remarkable difference on the level that P is less than 0.05. Wherein the formula LW-10 (40 mu g of 6-methyl-5-hepten-2-one, 32 mu g of 1-octen-3-ol and 88 mu g of phenethyl acetate) has the best attraction effect, the average trapping amount is 196.67 +/-4.10 heads per trap, and the trapping amount is obviously higher than other proportions of three plant volatiles. Meanwhile, the luring effect of the single plant volatile matter on the mythimna separata is verified, and the result shows that the luring effect is not ideal.
It can be seen from this that: the attractant composition of the invention has the following advantages: (1) the pheromone attractant has stronger trapping capability, and the attracting effect is improved by 1 time compared with that of the pheromone attractant alone; (2) the operation is simple, and the lasting period is within 30 days, so that the activity is better; (3) the composition is environment-friendly, has no toxicity and harm to human bodies (4), has cheap and easily-obtained raw materials and small using amount, and can obviously reduce the prevention and treatment cost; (4) can be accurately applied to insect condition prediction and mass trapping, and has important significance for comprehensively preventing and treating the mythimna separata.
The above disclosure is only for the specific embodiment of the present invention, but the present invention is not limited thereto, and any variations that can be made by those skilled in the art should fall within the scope of the present invention.
Claims (10)
1. An attractant composition for controlling mythimna separata, which is characterized by comprising mythimna separata sex pheromone and plant volatile components.
2. The attractant composition for controlling armyworm according to claim 1, wherein the armyworm sex pheromone component is cis-9-tetradecene acetate and cis-7-dodecene acetate, and the plant volatile matter is one or more of 6-methyl-5-hepten-2-one, 1-octen-3-ol and phenylethyl acetate.
3. The attractant composition for controlling mythimna separata according to claim 1, wherein the mass ratio of the mythimna separata sex pheromone to plant volatiles is 1: 0.05-0.2; the plant volatile matter comprises the following components in percentage by mass: 10-35% of 6-methyl-5-heptylene-2-ketone, 0-25% of 1-octene-3-ol and 45-80% of phenethylacetate.
4. The attractant composition for controlling mythimna separata according to claim 3, wherein the mass of said mythimna separata sex pheromone is 1000 μ g, and the mass of said plant volatile matter is 50-200 μ g.
5. The attractant composition for controlling armyworm according to claim 3, wherein said armyworm sex pheromone comprises 900 μ g of cis-9-tetradecene acetate and 100 μ g of cis-7-dodecene acetate.
6. The attractant composition for controlling mythimna separata according to claim 3, wherein the mass of said mythimna separata sex pheromone is 1000 μ g, the mass of said plant volatile matter is 160 μ g, and it is composed of the following components by mass percent: 25% of 6-methyl-5-hepten-2-one, 20% of 1-octen-3-ol and 55% of phenethyl acetate.
7. A lure for controlling mythimna laurentii comprising the mythimna laurentii lure composition according to any one of claims 1 to 6.
8. The method for preparing the lure of the attractant composition for controlling Lawsonia inermis according to claim 1, which is characterized in that,
(1) selecting a lure type of a polyvinyl chloride capillary tube (with the length of 10mm and the inner diameter of 1.5mm) or a polyethylene small bottle (with the height of 2-2.5 cm, the diameter of 0.5-0.6 cm and the thickness of 0.02-0.03 cm);
(2) diluting the Lawsonia inermis sex pheromone and plant volatile matter of the above claims with chlorinated paraffin or refined corn oil to gradient concentration, sucking a certain volume (10-150 μ L) according to dosage, and adding into the lure core;
(3) and (4) placing the prepared lure in a refrigerator at the temperature of-20 ℃ for storage for later use.
9. A method of using an attractant composition for controlling mythimna laurentii according to claim 1 characterised in that the attractant core is placed over a bucket type trap, the height of the trap is selected to be 1.0 to 1.5m and the average spacing is between 20 and 30 m.
10. Use of the attractant composition according to claim 1 for the biological control of mythimna laurenci.
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Cited By (2)
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| CN115380903A (en) * | 2022-09-17 | 2022-11-25 | 中国农业科学院植物保护研究所 | Lawsonia myxobolus sex pheromone synergist and preparation method and application thereof |
| CN115517254A (en) * | 2022-10-17 | 2022-12-27 | 中国农业科学院深圳农业基因组研究所 | High-efficiency attractant for mythimna lonella and application thereof |
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| CN115517254B (en) * | 2022-10-17 | 2024-03-19 | 中国农业科学院深圳农业基因组研究所 | Efficient attractant for myxoplasma gondii and application thereof |
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