CN111418589A - High-efficiency attractant for mythimna lonella and application thereof - Google Patents
High-efficiency attractant for mythimna lonella and application thereof Download PDFInfo
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- CN111418589A CN111418589A CN202010373998.5A CN202010373998A CN111418589A CN 111418589 A CN111418589 A CN 111418589A CN 202010373998 A CN202010373998 A CN 202010373998A CN 111418589 A CN111418589 A CN 111418589A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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Abstract
The invention discloses a high-efficiency attractant for Lawsonia inermis and application thereof. The high-efficiency attractant for the armyworm comprises cis-9-tetradecene acetate and cis-7-dodecene acetate, and the mass percentages of the two components are as follows: 50-90% of cis-9-tetradecene acetate and 10-50% of cis-7-dodecene acetate. The high-efficiency mythimna separata attractant can more effectively trap and kill the male mythimna separata, reduces the interspecies reproduction rate, has small using amount and is environment-friendly, can also be accurately applied to insect condition observation and prediction, a large amount of trapping and mating interference, and has important significance for the comprehensive treatment of the mythimna separata.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to a high-efficiency attractant for controlling mythimna separata and application thereof.
Background
The Lawsonia inermis (L eucania loreyi Duponche) belongs to Lepidoptera, noctuidae and is an important economic crop pest which is widely distributed in the world, mainly distributed in Africa, Australia, Asia, middle east and the like, is one of main pests in corn production in China, and is mainly distributed in provinces such as Henan, Guangdong, Yunnan, Fujian, Sichuan, Gansu and Taiwan of Zhejiang.
The existing method for preventing and controlling the Claviciae lauriformis mainly comprises chemical prevention and control and light trapping. Chemical pesticides bring huge pollution to human beings and the environment, excessive use easily causes resistance to the Lawsonia armyworm, the method of trapping and killing by utilizing lamplight has poor selectivity, has certain influence on beneficial insects such as natural enemies and the like, and has certain limitation in use due to reasons such as weather, geographical environment and the like. Therefore, the search for new methods and new technologies for pest control with economy, high efficiency and good environmental compatibility is an urgent need for solving the current environmental pollution and food safety crisis. Insect sex pheromones are known as third-generation pesticides and have green concepts of trace, no pollution, low residue and the like, so that the insect sex pheromones have wide application prospect in pest control. According to the principle that sex pheromone released by female insects has strong attraction capacity to the same kind of male insects, the components and the proportion of the sex pheromone of the Claviciae laurensis are clear and can be accurately applied to insect condition observation and prediction, pest quarantine, mating interference and mass trapping, and the ideal effect of comprehensively treating the Claviciae laurensis is achieved.
Disclosure of Invention
The invention aims to provide a high-efficiency attractant for preventing and controlling mythimna laurenci.
A high-efficiency attractant for preventing and controlling Lawsonia inermis comprises cis-9-tetradecene acetate and cis-7-dodecene acetate.
Optionally, the efficient attractant comprises the following components in percentage by mass: 50% -90% of cis-9-tetradecene acetate and 10% -50% of cis-7-dodecene acetate.
Preferably, the efficient attractant comprises the following components in percentage by mass: 75% -90% of cis-9-tetradecene acetate and 10% -25% of cis-7-dodecene acetate.
Preferably, the efficient attractant comprises the following components in percentage by mass: cis-9-tetradecene acetate 90%, cis-7-dodecene acetate 10%.
The invention also provides a lure for preventing and controlling the mythimna separata, which comprises the high-efficiency attractant for the mythimna separata.
The invention also provides application of the high-efficiency attractant for preventing and controlling the myxoma laevigata in the aspect of preventing and controlling the myxoma laevigata.
The application method of the high-efficiency attractant for the armyworm comprises the steps of mixing 1000 mu g of the high-efficiency attractant according to a ratio, diluting the mixture with chromatographically pure hexane, adding the diluted mixture into a reversed-mouth rubber head to prepare an attraction core, and then placing the attraction core above a barrel-shaped trap, wherein the distance between the trap and the ground is 1.5m, and the average distance is 10-15 m.
The invention has the beneficial effects that: 1. the using amount is less, and a large amount of armyworm males can be lured; 2. is environment-friendly and non-toxic and harmless to human body; 3. the raw materials are cheap and easy to obtain, and the economic cost is low; 4. the resistance of the Clavicia laurentii is not easy to generate; 5. the operation is simple, the use is convenient, and the lasting period is long; 6. can be accurately applied to insect condition detection and prediction, mass trapping and mating interference, and has important significance for comprehensively treating the myxoma laevigatae.
Drawings
FIG. 1 is a schematic diagram of the chemical structure of cis-9-tetradecene acetate.
FIG. 2 is a schematic diagram of the chemical structure of cis-7-dodecene acetate.
FIG. 3 is a scheme for the synthesis of cis-9-tetradecene acetate.
Detailed Description
The following detailed description of the present invention is provided in conjunction with the accompanying drawings, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Example 1 Synthesis of cis-9-tetradecene acetate
Taking a 100m L single-neck round-bottom flask, sequentially adding cis-9-tetradecene-1-ol (1.0g, 4.71mmol), triethylamine (0.57g, 5.65mmol), 4-dimethylamino pyridine (0.02g, 1% -5% wt) and dichloromethane (25m L), stirring under an ice salt bath, then dropwise adding acetic anhydride (0.58g, 5.65mmol), reacting for 30min, removing the ice salt bath, naturally heating to room temperature, stirring overnight, monitoring by T L C that the raw materials are not reduced, quenching the reaction by deionized water, washing by saturated saline water (25m L× 3), drying by anhydrous magnesium sulfate, concentrating under reduced pressure, and carrying out column chromatography [ eluent: V (ethyl acetate): V (petroleum ether): 1:30] to obtain 1.03g of colorless oily liquid, namely cis-9-tetradecene acetate with the yield of 86%.
The physical properties and yield of cis-9-tetradecene-1-alcohol acetate,1H NMR、13C NMR and HR-MS data are as follows:
(Z) -9-Tetradecenyl alcohol, a colorless oily liquid, yield 86%;1H NMR(300MHz,CDCl3):5.38-5.33(m,2H),4.06(t,J＝7.5Hz,2H),2.04-1.98(m,7H),1.63-1.57(m,2H),1.35-1.30(m,14H),0.85-0.91(m,2H)；13C NMR(75MHz,CDCl3):170.2,130.4(2C),64.9,32.0,29.9-25.8(8C),22.6,20.7,14.0；HR-MS(ESI)m/z:Calcd for C16H31O2{[M+H]+}254.2231,found 254.2218。
EXAMPLE 2 Synthesis of cis-7-dodecene acetate
The synthesis method is the same as example 1.
The physical properties and yield of cis-7-dodecene-1-alcohol acetate,1H NMR、13C NMR and HR-MS data are as follows:
(Z) -7-Dodecenyl alcohol, colorless oily liquid, yield 93%;1H NMR(300MHz,CDCl3):5.38-5.30(m,2H),4.05(t,J＝4.3Hz,2H),2.02-2.04(m,7H),1.69-1.57(m,2H),1.34-1.26(m,10H),0.93-0.89(m,3H)；13C NMR(75MHz,CDCl3):170.1,130.6(2C),64.9,32.1,29.7-25.9(6C),22.4,20.6,14.1；HR-MS(ESI)m/z:Calcd for C14H27O2{[M+H]+}226.1913,found226.1902。
example 3 field bioactivity assay
Preparing a lure:
the method comprises the steps of selecting a green reversed-mouth rubber head (14 mm in height, 8mm in inner diameter of an opening end, 1mm in wall thickness of the opening end and 5mm in opening depth) for a test lure, soaking the green reversed-mouth rubber head with 95% ethanol before adding an attractant, weighing a certain amount of two components of the attractant according to a proportion, uniformly mixing, adding a chromatographic pure hexane solution to prepare a mother solution of 10 mu g/mu L, sucking a solution of 100 mu L by a liquid transfer gun, adding the solution into the green reversed-mouth rubber head, adding 100 mu L of normal hexane after the solution completely permeates into rubber, volatilizing the normal hexane completely to obtain a lure containing 1000 mu g of the attractant, placing the lure in a refrigerator at-20 ℃ before the test, storing for later use, setting 5 repetitions for each lure treatment, setting blank treatment (CK) as a reference, and barrel-type traps containing live female Lawsonia armyworm (PE) which are not mated for 3-5 days for three times as a control lure, and numbering the treatments with different formulas as shown in Table 1.
Table 1 treatment numbers of different formulations
The corn fields with more serious Rous armyworm damage are selected for testing, and the traps are barrel-type traps which are 1.5m away from the ground and have the average spacing of 10-15 m.
The recording method comprises the following steps: the number of traps per trap was recorded every three days and the trap positions were randomly changed to eliminate trial errors due to position effects for 30 consecutive days. The test results are shown in table 2.
TABLE 2 trapping test results of different formulations for mythimna laurenci
Different lower case letters indicate significant differences at the P < 0.05 level in the Duncan method significance test
As can be seen from the results in Table 2, the attractant of the present invention has a good trapping effect on Cladosiphon immitis. As is clear from the comparison of significance, the attracting effect of the No. A3 (cis-9-tetradecene acetate 900. mu.g, cis-7-dodecene acetate 100. mu.g) is the most desirable, and the attracting effect is significantly different from the average trapping amount of other formulations (P < 0.05), and the average trapping amount is 106.00 +/-8.71 heads/trap.
The high-efficiency attractant can more effectively trap and kill the armyworm male moths, reduce the reproduction rate and gradually control the population base number of the pests, thereby achieving the purpose of preventing and controlling the armyworm. The invention has the advantages of less consumption, environmental friendliness, accurate application in insect condition detection and prediction, mass trapping and mating interference, and important significance in comprehensive treatment of the mythimna separata.
The above disclosure is only for the specific embodiment of the present invention, but the present invention is not limited thereto, and any variations that can be made by those skilled in the art should fall within the scope of the present invention.
Claims (7)
1. A high-efficiency attractant for Clavicia lauriformis, which is characterized in that the high-efficiency attractant comprises cis-9-tetradecene acetate and cis-7-dodecene acetate.
2. The efficient attractant for the armyworm according to claim 1, wherein the efficient attractant comprises the following components in percentage by mass: 50% -90% of cis-9-tetradecene acetate and 10% -50% of cis-7-dodecene acetate.
3. The efficient attractant for the armyworm according to claim 1, wherein the efficient attractant comprises the following components in percentage by mass: 75% -90% of cis-9-tetradecene acetate and 10% -25% of cis-7-dodecene acetate.
4. The efficient attractant for the armyworm according to claim 1, wherein the efficient attractant comprises the following components in percentage by mass: cis-9-tetradecene acetate 90%, cis-7-dodecene acetate 10%.
5. A lure for controlling mythimna separata comprising the high potency attractant for mythimna separata according to any one of claims 1 to 4.
6. Use of a potent attractant for myxoma lawsonii according to any one of claims 1 to 4 for controlling myxoma lawsonii.
7. The method for using the high-efficiency attractant for the Clay beetle according to any one of claims 1 to 4, wherein 1000 μ g of the high-efficiency attractant is mixed according to a proportion, diluted by chromatographically pure hexane and added into a reversed-mouth rubber head to form an attraction core, and then the attraction core is placed above a barrel-shaped trap, wherein the distance between the trap and the ground is 1.5m, and the average distance between the trap and the ground is 10-15 m.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113068694A (en) * | 2021-03-30 | 2021-07-06 | 中国农业科学院植物保护研究所 | Attractant composition for preventing and treating mythimna laurenci and application thereof |
| CN115380903A (en) * | 2022-09-17 | 2022-11-25 | 中国农业科学院植物保护研究所 | Lawsonia myxobolus sex pheromone synergist and preparation method and application thereof |
| CN115517254A (en) * | 2022-10-17 | 2022-12-27 | 中国农业科学院深圳农业基因组研究所 | High-efficiency attractant for mythimna lonella and application thereof |
-
2020
- 2020-05-06 CN CN202010373998.5A patent/CN111418589A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| M.D.CHISHOLM等: "FIELD TRAPPING OF DIAMONDBACK MOTH Plutella xylostella USING AN IMPROVED FOUR-COMPONENT SEX ATTRACTANT BLEND", 《JOURNAL OF CHEMICAL ECOLOGY》 * |
| Y.SATO ET AL.: "Attractiveness of the synthetic sex pheromone to the males of the armyworm, Leucania separata Walker and the lorey leafworm, Leucania loreyi Duponchel (Lepidoptera: Noctuidae)", 《APPL.ENT.ZOOL.》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113068694A (en) * | 2021-03-30 | 2021-07-06 | 中国农业科学院植物保护研究所 | Attractant composition for preventing and treating mythimna laurenci and application thereof |
| CN115380903A (en) * | 2022-09-17 | 2022-11-25 | 中国农业科学院植物保护研究所 | Lawsonia myxobolus sex pheromone synergist and preparation method and application thereof |
| CN115517254A (en) * | 2022-10-17 | 2022-12-27 | 中国农业科学院深圳农业基因组研究所 | High-efficiency attractant for mythimna lonella and application thereof |
| CN115517254B (en) * | 2022-10-17 | 2024-03-19 | 中国农业科学院深圳农业基因组研究所 | Efficient attractant for myxoplasma gondii and application thereof |
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Application publication date: 20200717 |