CN111109281A - Regional Spodoptera frugiperda attractant for Guangdong region - Google Patents

Regional Spodoptera frugiperda attractant for Guangdong region Download PDF

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CN111109281A
CN111109281A CN201911284177.8A CN201911284177A CN111109281A CN 111109281 A CN111109281 A CN 111109281A CN 201911284177 A CN201911284177 A CN 201911284177A CN 111109281 A CN111109281 A CN 111109281A
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spodoptera frugiperda
attractant
regional
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guangdong
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CN111109281B (en
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戴建青
陈大嵩
吴华
黄鸿
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Guangdong Institute of Applied Biological Resources
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N49/00Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02Saturated carboxylic acids or thio analogues thereof; Derivatives thereof

Abstract

The invention discloses a regional Spodoptera frugiperda attractant for Guangdong regions. The regional spodoptera frugiperda attractant consists of spodoptera frugiperda sex pheromones and plant volatile matters, wherein the mass ratio of the spodoptera frugiperda sex pheromones to the plant volatile matters is 1: 800-1000, wherein the Spodoptera frugiperda sex pheromone consists of cis-9-tetradecyl acetate and cis-7-dodecenyl acetate, and the plant volatile matter consists of phenylacetaldehyde and linalool. The attractant for regional Spodoptera frugiperda in Guangdong has strong specificity, can be compatible with any other prevention and control technologies, is not easy to generate pest resistance to drugs, is safe to people and livestock, does not pollute the environment, does not harm natural enemies, has low toxicity, no harm, trace quantity, high efficiency, simple and convenient operation, low price and easy industrialization technically, and can be widely used for prediction and prevention of invasion of important pests Spodoptera frugiperda and pest safety prevention and control.

Description

Regional Spodoptera frugiperda attractant for Guangdong region
The technical field is as follows:
the invention relates to the technical field of pest control, in particular to a regional Spodoptera frugiperda attractant in Guangdong regions.
Background art:
spodoptera frugiperda (Smith), also known as fall armyworm, belongs to the Lepidoptera (Lepidotera), Spodoptera frugiperda (Noctuidae). Native to the tropics and subtropical regions of the americas, widely distributed in the continental americas, are a local important agricultural pest. With the increasing frequency of international trade activities, Spodoptera frugiperda has occurred in nearly 100 countries worldwide, and is a major migratory pest across the national world for global early warning of food and agriculture organizations in the United nations. The insect released by the agricultural rural part migrates to the southwest and south China and quickly flies and spreads to the north, which poses serious threat to the food production of China.
Spodoptera frugiperda has the characteristics of host universality and serious damage. Spodoptera frugiperda is a polyphagic pest which can damage more than 80 plants, is loved to gramineae, and is found to be the most serious to damage corns. Spodoptera frugiperda also has the growth adaptability and migration diffusivity, and the generation length of the Spodoptera frugiperda is related to the environmental temperature and host plants. The Spodoptera frugiperda imagoes can fly in a long distance and directionally in the air of hundreds of meters by means of wind power, and can fly for 100km every night. The Spodoptera frugiperda monitoring, prevention and control work is deployed nationwide in the rural part of agriculture. The general investigation and the monitoring and early warning of the insect situation are well done, and the damage condition of the insect to the planting areas of gramineous crops such as corn, sugarcane, rice, sorghum and the like is mainly investigated. In the face of the outbreak of the important invasive pests, the development of a novel, safe and efficient pest forecasting and controlling technology is urgently needed to realize emergency prevention and control of the pests.
The insect sex pheromone technology can be applied to the prediction and control of the insect pests Spodoptera frugiperda, and is an important technical means in a safe and ecological pest control technical system. The insect pheromone pest control technology has the advantages of economy, high efficiency, no pollution to the environment, safety to natural enemies, no resistance problem to the environment and food safety and the like, can reduce the times of chemical pesticide control and the dosage of chemical pesticides, reduce the pesticide residue, and protect the ecological environment and the safe production of agricultural products.
The invention content is as follows:
the invention aims to provide a regional spodoptera frugiperda attractant in Guangdong, which has good attraction effect on spodoptera frugiperda in Guangdong and can be widely applied to pest forecasting and prevention and control of spodoptera frugiperda.
The invention aims to provide a regional spodoptera frugiperda attractant in Guangdong region, which consists of spodoptera frugiperda sex pheromone and plant volatile matters, wherein the mass ratio of the spodoptera frugiperda sex pheromone to the plant volatile matters is (1): 800-1000, wherein the Spodoptera frugiperda sex pheromone consists of cis-9-tetradecyl acetate and cis-7-dodecenyl acetate, and the plant volatile matter consists of phenylacetaldehyde and linalool.
In the regional spodoptera frugiperda attractant in Guangdong, the spodoptera frugiperda sex pheromone and the plant volatile matter are matched with each other, so that the synergistic interaction effect is achieved, and the trapping effect of the attractant on spodoptera frugiperda is improved.
Preferably, the mass ratio of the cis-9-tetradecene acetate to the cis-7-dodecene acetate is 90-99.9: 0.1 to 10.
Preferably, the mass ratio of cis-9-tetradecene acetate to cis-7-dodecene acetate is 99.5: 0.5.
preferably, the volume ratio of phenylacetaldehyde to linalool is 1: 1.
The invention develops a regional attractant suitable for spodoptera frugiperda in Guangdong region aiming at spodoptera frugiperda in Guangdong region, the attractant has good attraction effect on spodoptera frugiperda in Guangdong region, and the optimal formula is as follows: the mass ratio of Spodoptera frugiperda sex pheromone to plant volatile matter is 1: 800-1000, wherein the mass ratio of cis-9-tetradecyl acetate to cis-7-dodecenyl acetate in the Spodoptera frugiperda sex pheromone is 99.5: 0.5, the regional Spodoptera frugiperda attractant in Guangdong region can be widely applied to pest prediction and control of Spodoptera frugiperda.
The invention also provides application of the regional Spodoptera frugiperda attractant in Guangdong regions in trapping Spodoptera frugiperda.
The invention also provides a lure of the regional Spodoptera frugiperda attractant in Guangdong region, which comprises the regional Spodoptera frugiperda attractant in Guangdong region, wherein the mass ratio of Spodoptera frugiperda sex pheromone to plant volatile matter in each lure is 1: 800 to 1000.
The invention has the beneficial effects that: the attractant for regional Spodoptera frugiperda in Guangdong has strong specificity, can be compatible with any other prevention and control technologies, is not easy to generate pest resistance to drugs, is safe to people and livestock, does not pollute the environment, does not harm natural enemies, has low toxicity, no harm, trace quantity, high efficiency, simple and convenient operation, low price and easy industrialization technically, and can be widely used for prediction and prevention of invasion of important pests Spodoptera frugiperda and pest safety prevention and control.
The specific implementation mode is as follows:
the present invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. In practice, the technical personnel according to the invention make improvements and modifications, which still belong to the protection scope of the invention.
The equipment and reagents used in the present invention are, unless otherwise specified, conventional commercial products in the art.
The following examples are directed to regional spodoptera frugiperda attractant in the Guangdong region, which is prepared from spodoptera frugiperda sex pheromone and plant volatile matters according to the mass ratio of 1: 800-1000 parts; the Spodoptera frugiperda sex pheromone is prepared from cis-9-tetradecyl acetate and cis-7-dodecenyl acetate according to a mass ratio of 90-99.9: 0.1-10, wherein the plant volatile matter is prepared from phenylacetaldehyde and linalool according to a volume ratio of 1: 1.
The specific test mixture ratio is shown in table 1, and the table 1 shows the components and the contents of the components in each test group.
The preparation process comprises the following steps: the spodoptera frugiperda pheromone lure core is made of a silicon rubber plug, is red, has the diameter of 0.9cm and the height of 0.9cm, and has the volume of about 300 mu L. Before use, the spodoptera frugiperda pheromone lure core is prepared, cis-9-tetradecene acetate and cis-7-dodecene acetate are dissolved in dichloromethane according to the mass ratio to prepare dichloromethane solution with a certain concentration, the dichloromethane solution is quantitatively added into a concave cup of a silicon rubber plug, and after dichloromethane is completely volatilized, the dichloromethane is stored at a low temperature for later use. The sex pheromone lure of Spodoptera frugiperda used in the test was 200. mu.g/lure. Inducing the plant volatile matter into a core by using a plastic centrifugal tube, and mixing phenylacetaldehyde and linalool according to a volume ratio of 1:1, adding into a centrifuge tube, and using 100 mu L of two plant volatile matters. During the test, the spodoptera frugiperda pheromone lure and the plant volatile matter lure are fixed together to form a group for use.
The trap adopts a triangular sticky insect type trap, and the upper part of the trap plate is fixed with a trapping core by a thin iron wire. The trap is placed at a height above the crop or level with the crop. The experimental design was as follows:
table 1: components and contents of the test groups
Figure BDA0002317552990000041
Example 1:
the field trapping test was conducted in a corn field in the Zea mays region of Guangzhou City. The spodoptera frugiperda luring core is placed on the upper portion of the luring plate, and the height of the trapper is equal to the middle upper portion of the crop or the level of the trapper and the crop. Experimental design a, B, C, D, E, F, G, H, I, J total 10 treatments (as shown in table 2), where H is a sex pheromone monocomponent control, I is a plant volatile component control, J is a blank control, 5 replicates of each treatment were set up in the field, and the pots were placed randomly in the field, with a distance of about 20m between each pot. The lured moth numbers were counted and the lured adult noctuids were brought back to the laboratory for further identification, investigated every 2-3 days for each different treatment. Meanwhile, the trap is replaced, so that the influence of the position on the moth trapping effect is reduced, and the pest sticking plate of the trap is timely replaced according to the moth trapping condition.
Table 2: comparison of field moth-luring effects of Spodoptera frugiperda sex attractants with different proportions
Figure BDA0002317552990000051
The test results are shown in table 2, and experiments show that the attractant for regional spodoptera frugiperda in Guangdong regions of the invention with different formulas has an attracting effect on spodoptera frugiperda adults which are obviously higher than that of blank controls, wherein the spodoptera frugiperda adults trapped by the formula D have the largest number, the total number of the attracted moths reaches 31, and the attracting effect is better than that of other formulas. Compared with the single-component spodoptera frugiperda sex pheromone attractant formulas F and G, the spodoptera frugiperda sex pheromone attractant has the advantages that the quantity of the spodoptera frugiperda sex pheromone attractant is respectively increased by 1.58 times and 2.88 times, and the field spodoptera frugiperda sex pheromone attractant is improved.
Example 2:
the field trapping trials were conducted in Guangdong corn fields. Experimental design a, B, C, D, E, H, I, J total 8 treatments (as shown in table 3), each treatment was set up in 5 replicates in the field, traps were placed randomly in the field, and each trap was approximately 20m apart. Other methods were performed in the same manner as in example 1, and the number of moth-lured insects was counted by examining every 2 to 5 days. Clear away simultaneously and lure the meadow spodoptera imago caught, change the position that lures the basin and reduce the influence of position to luring the moth effect to in time change trapper mythimna separata board according to luring the moth condition.
Table 3: comparison of field moth-luring effects of Spodoptera frugiperda sex attractants with different proportions
Figure BDA0002317552990000061
The test results are shown in table 3, and experiments show that the luring effect of the spodoptera frugiperda attractant of several different formulas is obviously higher than that of a blank control on spodoptera frugiperda adults, wherein the total number of the spodoptera frugiperda adults lured by the formula D is 30, the moth-luring effect is better than that of other attractant formulas, and spodoptera frugiperda sex pheromones and plant volatile matters are matched with each other, so that the synergistic effect is achieved, and the luring effect of the attractant on spodoptera frugiperda is improved.
As can be seen from Table 3, compared with other Spodoptera frugiperda attractant formulas, the formula D of the attractant in Guangdong region, disclosed by the invention, has the highest attraction quantity of Spodoptera frugiperda, so that the aim of developing and developing a regional specific efficient Spodoptera frugiperda attractant is fulfilled, and a technical support is provided for the application of the regional specific efficient Spodoptera frugiperda attractant in the Spodoptera frugiperda regional ecological control technology.
The above detailed description is specific to one possible embodiment of the present invention, and the embodiment is not intended to limit the scope of the present invention, and all equivalent implementations or modifications that do not depart from the scope of the present invention are intended to be included within the scope of the present invention.

Claims (6)

1. The regional spodoptera frugiperda attractant for the Guangdong region is characterized by consisting of spodoptera frugiperda sex pheromones and plant volatile matters, wherein the mass ratio of the spodoptera frugiperda sex pheromones to the plant volatile matters is 1: 800-1000, wherein the Spodoptera frugiperda sex pheromone consists of cis-9-tetradecyl acetate and cis-7-dodecenyl acetate, and the plant volatile matter consists of phenylacetaldehyde and linalool.
2. The regional spodoptera frugiperda attractant as claimed in claim 1, wherein the mass ratio of cis-9-tetradecene acetate to cis-7-dodecene acetate is 90-99.9: 0.1 to 10.
3. The regional spodoptera frugiperda attractant as claimed in claim 2, wherein the mass ratio of cis-9-tetradecene acetate to cis-7-dodecene acetate is 99.5: 0.5.
4. the regional spodoptera frugiperda attractant in the Guangdong area of claim 1, wherein the volume ratio of phenylacetaldehyde to linalool is 1: 1.
5. The use of the regional spodoptera frugiperda attractant of claim 1 for trapping spodoptera frugiperda.
6. A spodoptera frugiperda attractant lure for the regional spodoptera frugiperda in the Guangdong region, comprising the spodoptera frugiperda attractant for the regional spodoptera frugiperda in the Guangdong region of claim 1, wherein the mass ratio of spodoptera frugiperda sex pheromone to plant volatiles in each lure is 1: 800 to 1000.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111685118A (en) * 2020-07-09 2020-09-22 漳州市英格尔农业科技有限公司 Spodoptera frugiperda imago attractant and trapping device containing same
CN111955458A (en) * 2020-07-17 2020-11-20 北京依科曼生物技术股份有限公司 Insect pheromone attractant composition for spodoptera frugiperda
CN112075390A (en) * 2020-09-15 2020-12-15 广东省农业科学院植物保护研究所 Spodoptera frugiperda parasitic wasp propagation method
CN112400892A (en) * 2020-12-30 2021-02-26 福建省农业科学院植物保护研究所 Nano drug delivery system of sex attractant for Spodoptera frugiperda and application of nano drug delivery system
CN113331201A (en) * 2021-06-03 2021-09-03 中国农业科学院植物保护研究所 Attractant composition for preventing and treating spodoptera frugiperda and application thereof

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111685118A (en) * 2020-07-09 2020-09-22 漳州市英格尔农业科技有限公司 Spodoptera frugiperda imago attractant and trapping device containing same
CN111685118B (en) * 2020-07-09 2021-10-26 漳州市英格尔农业科技有限公司 Spodoptera frugiperda imago attractant and trapping device containing same
CN111955458A (en) * 2020-07-17 2020-11-20 北京依科曼生物技术股份有限公司 Insect pheromone attractant composition for spodoptera frugiperda
CN111955458B (en) * 2020-07-17 2021-10-15 北京依科曼生物技术股份有限公司 Insect pheromone attractant composition for spodoptera frugiperda
CN112075390A (en) * 2020-09-15 2020-12-15 广东省农业科学院植物保护研究所 Spodoptera frugiperda parasitic wasp propagation method
CN112400892A (en) * 2020-12-30 2021-02-26 福建省农业科学院植物保护研究所 Nano drug delivery system of sex attractant for Spodoptera frugiperda and application of nano drug delivery system
CN112400892B (en) * 2020-12-30 2021-11-09 福建省农业科学院植物保护研究所 Nano drug delivery system of sex attractant for Spodoptera frugiperda and application of nano drug delivery system
CN113331201A (en) * 2021-06-03 2021-09-03 中国农业科学院植物保护研究所 Attractant composition for preventing and treating spodoptera frugiperda and application thereof

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