CN112075390A - Spodoptera frugiperda parasitic wasp propagation method - Google Patents
Spodoptera frugiperda parasitic wasp propagation method Download PDFInfo
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- CN112075390A CN112075390A CN202010968985.2A CN202010968985A CN112075390A CN 112075390 A CN112075390 A CN 112075390A CN 202010968985 A CN202010968985 A CN 202010968985A CN 112075390 A CN112075390 A CN 112075390A
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- 241000257303 Hymenoptera Species 0.000 claims abstract description 20
- 238000012258 culturing Methods 0.000 claims abstract description 16
- 230000024241 parasitism Effects 0.000 claims abstract description 15
- 239000000126 substance Substances 0.000 claims abstract description 5
- 239000005667 attractant Substances 0.000 claims description 54
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- 235000013601 eggs Nutrition 0.000 claims description 45
- 238000009395 breeding Methods 0.000 claims description 36
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- 241000238631 Hexapoda Species 0.000 claims description 25
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention belongs to the technical field of Spodoptera frugiperda control and discloses a Spodoptera frugiperda parasitic wasp propagation method, which comprises the following steps: collecting egg masses laid by the spodoptera frugiperda imagoes, and transferring the egg masses into a room for artificial feeding; collecting the cocoons of the Spodoptera frugiperda parasitism bees, and culturing to enable the cocoons to emerge; selecting Spodoptera frugiperda egg masses, inoculating Spodoptera frugiperda parasitic wasps to the egg masses, and performing parasitic propagation of the parasitic wasps to obtain the wasp cocoons of the parasitic wasps; and (4) transporting the cocoons of the parasitic wasps to a Spodoptera frugiperda threat area for releasing. According to the method, the Spodoptera frugiperda is indoors fed as a propagation host of the Spodoptera frugiperda parasitic wasp, the imago periods of the Spodoptera frugiperda and the Spodoptera frugiperda are consistent, the Spodoptera frugiperda egg masses are used for parasitizing the Spodoptera frugiperda parasitic wasp imagoes, a large number of parasitic natural enemies are artificially produced and released in the field, and the harm of the Spodoptera frugiperda to; meanwhile, the use amount of chemical pesticides can be reduced, and the yield and the quality of agricultural products can be improved.
Description
Technical Field
The invention belongs to the technical field of Spodoptera frugiperda control, and particularly relates to a Spodoptera frugiperda parasitic wasp propagation method.
Background
At present: biological control is one of important measures in a green prevention and control means of pests, and natural enemy insects have important regulation and natural control functions on the population occurrence of the pests. Insects, the natural enemy of spodoptera frugiperda, are a wide variety of species worldwide. According to incomplete statistics, at least 10 parasitic wasps parasitizing Spodoptera frugiperda belong to 121 families, and because Spodoptera frugiperda is native to America, most natural enemy species are only distributed in America, and 16 parasitic wasps of Spodoptera frugiperda are recorded and distributed in China at present; the species of the Spodoptera frugiperda parasitism flies are also quite rich, and about 4 families are 66 in the world; predatory insects of spodoptera frugiperda are mainly concentrated in coleoptera, dermaptera and hemiptera. The method can provide basic resources for the application of the natural enemies of the Spodoptera frugiperda in future and lay a foundation for screening the dominant natural enemies of the Spodoptera frugiperda and large-scale breeding of the dominant natural enemies of the Spodoptera frugiperda.
Parasitic wasps are the most common type of parasitic insects and are natural enemies of a variety of agricultural and forestry pests. The parasitic wasp is parasitic in larva, pupa and egg of lepidoptera, coleoptera, hymenoptera, diptera and other insects, and can kill parasitic insects, so that insect pests on crops can be indirectly reduced. Therefore, the number of the parasitic wasps in the field is increased by a method of artificially expanding the parasitic wasps and then releasing the parasitic wasps in the field, and the method has obvious significance of killing pests, reducing the pesticide consumption and protecting the environment. However, the existing method for expanding and breeding parasitic wasps needs to put larvae required by the oviposition of the parasitic wasps into a bottle and then put the parasitic wasps into the bottle; the bee pupae are taken out after spawning or pupating, and have the defects of troublesome operation, inconvenient collection of the bee pupae, slow propagation expanding speed and more flying escape of parasitic bees. Meanwhile, the existing parasitic wasp research is mainly carried out on trichogramma and aphelenchoides besseyi, and no related research for controlling the population quantity of target pests by utilizing Taiwan abdominal cocoon bee exists at present.
Through the above analysis, the problems and defects of the prior art are as follows: the existing method for expanding and breeding parasitic wasps needs to put larvae required by the oviposition of the parasitic wasps into a bottle and then put the parasitic wasps into the bottle; the bee pupae are taken out after spawning or pupating, and have the defects of troublesome operation, inconvenient collection of the bee pupae, slow propagation expanding speed and more flying escape of parasitic bees. Meanwhile, the prior art does not have a propagation expanding method for Taiwan abdominal cocoon bee.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a Spodoptera frugiperda parasitism bee propagation method.
The invention is realized in such a way that the Spodoptera frugiperda parasitic wasp propagation method comprises the following steps:
the method comprises the following steps of firstly, carrying out indoor culture and propagation of spodoptera frugiperda, luring the spodoptera frugiperda, and taking imagoes of the spodoptera frugiperda for propagation of parasitic wasps of the spodoptera frugiperda;
the method for luring spodoptera frugiperda comprises the following steps:
(1.1) placing spodoptera frugiperda attractors in a spodoptera frugiperda culture area, and placing attractant pieces in the spodoptera frugiperda attractors;
(1.2) placing the spodoptera frugiperda attractant above plants in an attraction area at the night of clear and cloudy days, and enabling the bottom of the attractant to be higher than the top of the plants;
(1.3) opening a spodoptera frugiperda attractant, adopting a method of combining chemical attraction and physical attraction to attract the spodoptera frugiperda imagoes, and collecting the attracted spodoptera frugiperda imagoes;
step two, continuously culturing the spodoptera frugiperda imagoes for 2-3 days, and collecting egg masses produced by the imagoes;
the method comprises the following steps of continuously culturing the spodoptera frugiperda imagoes for 2-3 days, and collecting egg masses laid by the imagoes:
(2.1) distinguishing female and male of the attracted spodoptera frugiperda imagoes, and then, according to female: culturing male in a ratio of 1:1 in an artificial climate incubator;
(2.2) placing two sterilized culture dishes paved with sterilized filter paper in an artificial climate incubator, wherein the culture dishes are respectively used for placing feed, laying eggs and placing clear water; replacing the sterilized filter paper, the feed and the clear water every 8 hours;
(2.3) mating and laying eggs for the adult insects, replacing a culture dish for laying eggs with a new feed culture dish, and taking out and collecting egg masses laid by the adult insects;
thirdly, transferring the collected egg masses into a room for artificial feeding;
1) putting the collected egg masses and the plastic strips into a culture tank for 1-2 days for culture;
2) putting Spodoptera frugiperda breeding feed into the culture tank, and putting the egg mass and the plastic strip above the breeding feed;
3) placing the culture tank into an incubator, setting the culture temperature at 23-26 ℃ and the humidity at 60-65%, and incubating spodoptera frugiperda eggs;
4) feeding after the hatching is finished, wherein the feeding temperature is 25-27 ℃, and the humidity is 60-70%;
5) when the Spodoptera frugiperda pupae becomes hard and dark brown, taking the pupae out of the culture tank, putting the pupae into a plastic box, and performing eclosion in a dark environment;
collecting the cocoons of the Spodoptera frugiperda parasitism bees, and culturing for 12-14 days to enable the cocoons to emerge;
step five, selecting 1-day-old Oncorhynchus Spodoptera frugiperda egg blocks, inoculating parasitic Oncorhynchus Spodoptera bees to the egg blocks, placing the egg blocks in a bee breeding box after inoculating the parasitic Oncorhynchus Spodoptera bees, covering a box cover of the bee breeding box, placing the bee breeding box on a bee breeding frame, and culturing offspring bees;
step six, carrying out parasitic propagation of parasitic wasps to ensure that the parasitic wasps spin cocoons to obtain the cocoons of the parasitic wasps;
and seventhly, storing the cocoons of the parasitic wasps in an environment of 20-23 ℃, and transporting the cocoons to a Spodoptera frugiperda threat area for releasing.
Further, in the step one, before luring spodoptera frugiperda, waiting for 7-10 days is needed, and the spodoptera frugiperda pupae becomes spodoptera frugiperda imagoes after eclosion.
Further, in the step (1.1), the spodoptera frugiperda attractant is provided with:
the device comprises a support rod, an attractant shell, a transparent insect collector, an attractant sheet placing groove, an attractant channel and a blocking cover;
the lure device shell is cylindrical and is connected with the support rod through a bolt; the bottom end of the attractant shell is provided with a transparent insect collector, the middle position of the upper end of the attractant shell is provided with an attractant sheet placing groove, and insect attracting channels are arranged around the attractant sheet placing groove; a blocking cover is arranged 1-2cm above the insect attracting passage, and the diameter of the blocking cover is consistent with that of the attractant shell.
Further, in the step (1.1), the attractant sheet comprises, by mass, (Z) -7-dodecen-1-ol acetate 1-2 parts, (Z) -9-tetradecene acetate 1-2 parts, (Z) -11-hexadecene alcohol acetate 2-3 parts, (Z) -9-dodecen-1-ol acetate 1-3 parts, phenylacetaldehyde 300-400 parts, linalool 400-600 parts, a dispersing agent 6-8 parts, a slow release agent 10-25 parts, and water 40-50 parts.
Further, in the step (1.1), the method for preparing the attractant piece comprises the following steps:
(1.1.1) mixing (Z) -7-dodecen-1-ol acetate, (Z) -9-tetradecene acetate, (Z) -11-hexadecene alcohol acetate, (Z) -9-dodecen-1-ol acetate according to corresponding parts by mass to obtain a mixture A;
(1.1.2) mixing phenylacetaldehyde and linalool according to the mass parts to obtain a mixture B;
(1.1.3) adding a dispersing agent into the mixture B, adding water, and uniformly stirring to obtain a mixture C;
(1.1.4) slowly pouring the mixture A into the mixture C, continuously stirring, and adding a slow release agent during stirring to obtain an attractant;
(1.1.5) dropwise adding the attractant into the mold, putting the mold into a drying oven, and drying at the set drying temperature of 50-55 ℃ for 25-35min to obtain the attractant sheet.
Further, in the step (2.1), the temperature of the artificial climate incubator is 28 ℃, the RH is 78%, and the ratio of L: d ═ 16: 8.
further, in the step 2), the preparation method of the spodoptera frugiperda feeding feed is characterized by comprising the following steps:
step a, boiling distilled water, and adding edible agar to completely dissolve the edible agar to obtain agar liquid;
step b, mixing the wheat bran and the corn flour, adding distilled water, and uniformly stirring;
c, adding agar liquid into the mixed liquid, uniformly stirring, standing and cooling;
and d, after the temperature is reduced to below 60 ℃, adding glucose, yeast and vitamin B, and uniformly stirring to obtain the spodoptera frugiperda feeding feed.
Further, in the fourth step, the Spodoptera frugiperda parasitism wasp is Taiwan Abelmoschus manihot.
Further, in the sixth step, the parasitic propagation of the parasitic wasps is specifically performed by: the method comprises the steps of firstly putting cultured Spodoptera frugiperda parasitism bee offspring bees into a breeding box, then putting Spodoptera frugiperda egg masses or larvae into the breeding box, then placing the breeding box into an artificial climate box with the temperature set to be 25 ℃ and the relative humidity of 70%, feeding the breeding box until the Spodoptera frugiperda parasitism bees bite into mature larvae of Spodoptera frugiperda, and then spinning cocoons to obtain the bee cocoons.
Further, in the sixth step, during the parasitic propagation of the parasitic wasps, 15% of honey water is adsorbed by cotton or sponge to provide nutrition for the breeding wasps, the temperature in the insect propagation box is controlled to be 23-26 ℃, the relative humidity is kept at 60-70%, and ventilation is kept.
By combining all the technical schemes, the invention has the advantages and positive effects that: the method is used for breeding Spodoptera frugiperda and Spodoptera frugiperda parasitic wasps respectively to obtain imagoes of the Spodoptera frugiperda and the Spodoptera frugiperda, so that the parasitic rate of the bred Spodoptera frugiperda parasitic wasps on Spodoptera frugiperda is realized, and the Spodoptera frugiperda is effectively prevented and treated; the Spodoptera frugiperda is bred indoors to serve as a propagation host of Spodoptera frugiperda parasitic wasps, the imago periods of the Spodoptera frugiperda and the Spodoptera frugiperda are consistent, the Spodoptera frugiperda egg masses are used for parasitizing the Spodoptera frugiperda parasitic wasps, a large number of parasitic natural enemies are artificially produced and released in the field, the population quantity of the Spodoptera frugiperda can be controlled, and the harm of the Spodoptera; meanwhile, the method can reduce the usage amount of chemical pesticides and improve the yield and quality of agricultural products.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
Fig. 1 is a flowchart of a spodoptera frugiperda parasitic wasp propagation method provided in an embodiment of the present invention.
FIG. 2 is a flow chart of a method for attracting Spodoptera frugiperda adults provided by the embodiment of the invention.
Fig. 3 is a flow chart of a method for preparing an attractant piece according to an embodiment of the present invention.
FIG. 4 is a flow chart of the artificial feeding procedure for moving collected egg masses into a room according to an embodiment of the present invention.
FIG. 5 is a flow chart of a method for preparing the Spodoptera frugiperda feeding feed provided by the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a Spodoptera frugiperda parasitism bee propagation method, and the invention is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the method for expanding and propagating spodoptera frugiperda parasitic wasps provided by the embodiment of the present invention includes the following steps:
s101, indoor culture and propagation of spodoptera frugiperda are carried out, and spodoptera frugiperda imagoes are taken to be used for propagation of spodoptera frugiperda parasitism bees.
S102, continuously culturing the spodoptera frugiperda imagoes for 2-3 days, and collecting egg masses produced by the imagoes.
S103, moving the collected egg masses into a room for artificial feeding.
S104, collecting the cocoons of the Spodoptera frugiperda parasitism bees, and culturing for 12-14 days to enable the cocoons to emerge.
S105, selecting 1-day-old spodoptera frugiperda egg blocks, inoculating spodoptera frugiperda parasitic wasps to the egg blocks, placing the egg blocks in a wasp breeding box after inoculating the wasps, covering a box cover of the wasp breeding box, placing the wasp breeding box on a wasp breeding frame, and culturing offspring wasps.
And S106, carrying out parasitic propagation of parasitic wasps to ensure that the parasitic wasps spin cocoons to obtain the cocoons of the parasitic wasps.
S107, storing the cocoons of the parasitic wasps in an environment of 20-23 ℃, and transporting the cocoons to a Spodoptera frugiperda threat area for releasing.
In step S101, before luring spodoptera frugiperda provided in the embodiment of the present invention, waiting for 7 to 10 days is required, and the spodoptera frugiperda pupae is eclosized into spodoptera frugiperda adults.
As shown in fig. 2, in step S101, the method for attracting spodoptera frugiperda provided in the embodiment of the present invention includes the following steps:
s201, placing spodoptera frugiperda attractors in a spodoptera frugiperda culture area, and placing attractant pieces in the spodoptera frugiperda attractors.
S202, at sunny and cloudy night, placing the spodoptera frugiperda attractant above plants in an attraction area, and enabling the bottom of the attractant to be higher than the top of the plants.
S203, opening the spodoptera frugiperda trapper, adopting a method of combining chemical trapping and physical trapping to trap the spodoptera frugiperda imagoes, and collecting the trapped spodoptera frugiperda imagoes.
In step S201, the spodoptera frugiperda attractant provided by the embodiment of the present invention includes:
the device comprises a support rod, an attractant shell, a transparent insect collector, an attractant sheet placing groove, an attractant channel and a blocking cover;
the lure device shell is cylindrical and is connected with the support rod through a bolt; the bottom end of the attractant shell is provided with a transparent insect collector, the middle position of the upper end of the attractant shell is provided with an attractant sheet placing groove, and insect attracting channels are arranged around the attractant sheet placing groove; a blocking cover is arranged 1-2cm above the insect attracting passage, and the diameter of the blocking cover is consistent with that of the attractant shell.
In step S201, the attractant sheet provided in the embodiment of the present invention comprises, by mass, (Z) -7-dodecen-1-ol acetate 1-2 parts, (Z) -9-tetradecene acetate 1-2 parts, (Z) -11-hexadecenol acetate 2-3 parts, (Z) -9-dodecen-1-ol acetate 1-3 parts, phenylacetaldehyde 300-400 parts, linalool 400-600 parts, a dispersant 6-8 parts, a sustained release agent 10-25 parts, and water 40-50 parts.
As shown in fig. 3, in step S201, the method for preparing an attractant sheet according to an embodiment of the present invention includes the following steps:
s301, mixing (Z) -7-dodecen-1-ol acetate, (Z) -9-tetradecene acetate, (Z) -11-hexadecenol acetate, and (Z) -9-dodecen-1-ol acetate according to corresponding parts by mass to obtain a mixture A;
s302, mixing phenylacetaldehyde and linalool according to parts by weight to obtain a mixture B;
s303, adding a dispersing agent into the mixture B, adding water, and uniformly stirring to obtain a mixture C;
s304, slowly pouring the mixture A into the mixture C, continuously stirring, and adding the slow release agent during stirring to obtain the attractant;
s305, dropwise adding the attractant into the mold, putting the mold into a drying oven, and drying the mold for 25-35min at a drying temperature of 50-55 ℃ to obtain the attractant sheet.
The method for continuously culturing the spodoptera frugiperda imagoes for 2-3 days comprises the following steps:
(2.1) distinguishing female and male of the attracted spodoptera frugiperda imagoes, and then, according to female: culturing male in a ratio of 1:1 in an artificial climate incubator;
(2.2) placing two sterilized culture dishes paved with sterilized filter paper in an artificial climate incubator, wherein the culture dishes are respectively used for placing feed, laying eggs and placing clear water; replacing the sterilized filter paper, the feed and the clear water every 8 hours;
(2.3) mating and laying eggs for the adult insects, replacing a culture dish for laying eggs with a new feed culture dish, and taking out and collecting egg masses laid by the adult insects;
in the step (2.1), the temperature of the artificial climate incubator provided by the embodiment of the invention is 28 ℃, the RH is 78%, and the L: d ═ 16: 8.
as shown in fig. 4, in step S103, the moving the collected egg mass into a room for artificial feeding according to the embodiment of the present invention includes:
s401, putting the collected egg masses and the plastic strips into a culture tank for 1-2 days of culture.
S402, putting Spodoptera frugiperda feeding feed into the culture tank, and putting the egg mass and the plastic strip above the feeding feed together.
S403, placing the culture tank into an incubator, setting the culture temperature to be 23-26 ℃ and the humidity to be 60-65%, and incubating spodoptera frugiperda eggs.
S404, breeding after hatching, wherein the breeding temperature is 25-27 ℃, and the humidity is 60-70%.
S405, when the Spodoptera frugiperda pupae becomes hard and dark brown, taking the pupae out of the culture tank, putting the pupae into a plastic box, and performing eclosion in a dark environment.
As shown in fig. 5, in step S402, the method for preparing a spodoptera frugiperda feeding feed provided by the embodiment of the present invention is characterized by comprising the following steps:
s501, boiling distilled water, and adding edible agar to completely dissolve the edible agar to obtain an agar liquid;
s502, mixing the wheat bran and the corn flour, adding distilled water, and uniformly stirring;
s503, adding the agar liquid into the mixed liquid, uniformly stirring, standing and cooling;
s504, after the temperature is reduced to below 60 ℃, adding glucose, yeast and vitamin B, and uniformly stirring to obtain the Spodoptera frugiperda feeding feed.
In step S104, the spodoptera frugiperda parasitic wasp provided by the embodiment of the present invention is a taiwan veropaea septoria.
In step S106, the parasitic propagation of the parasitic wasps provided in the embodiment of the present invention specifically includes: the method comprises the steps of firstly putting cultured Spodoptera frugiperda parasitism bee offspring bees into a breeding box, then putting Spodoptera frugiperda egg masses or larvae into the breeding box, then placing the breeding box into an artificial climate box with the temperature set to be 25 ℃ and the relative humidity of 70%, feeding the breeding box until the Spodoptera frugiperda parasitism bees bite into mature larvae of Spodoptera frugiperda, and then spinning cocoons to obtain the bee cocoons.
In step S106, during the parasitic propagation of the parasitic wasps, the cotton or sponge is used to adsorb 15% of the honey water to provide nutrition for the breeding wasps, the temperature in the insect propagation box is controlled to be 23-26 ℃, the relative humidity is kept at 60-70%, and ventilation is maintained.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.
Claims (10)
1. A Spodoptera frugiperda parasitoid bee propagation method is characterized by comprising the following steps:
the method comprises the following steps of firstly, carrying out indoor culture and propagation of spodoptera frugiperda, luring the spodoptera frugiperda, and taking imagoes of the spodoptera frugiperda for propagation of parasitic wasps of the spodoptera frugiperda;
the method for luring spodoptera frugiperda comprises the following steps:
(1.1) placing spodoptera frugiperda attractors in a spodoptera frugiperda culture area, and placing attractant pieces in the spodoptera frugiperda attractors;
(1.2) placing the spodoptera frugiperda attractant above plants in an attraction area at the night of clear and cloudy days, and enabling the bottom of the attractant to be higher than the top of the plants;
(1.3) opening a spodoptera frugiperda attractant, adopting a method of combining chemical attraction and physical attraction to attract the spodoptera frugiperda imagoes, and collecting the attracted spodoptera frugiperda imagoes;
step two, continuously culturing the spodoptera frugiperda imagoes for 2-3 days, and collecting egg masses produced by the imagoes;
the method comprises the following steps of continuously culturing the spodoptera frugiperda imagoes for 2-3 days, and collecting egg masses laid by the imagoes:
(2.1) distinguishing female and male of the attracted spodoptera frugiperda imagoes, and then, according to female: culturing male in a ratio of 1:1 in an artificial climate incubator;
(2.2) placing two sterilized culture dishes paved with sterilized filter paper in an artificial climate incubator, wherein the culture dishes are respectively used for placing feed, laying eggs and placing clear water; replacing the sterilized filter paper, the feed and the clear water every 8 hours;
(2.3) mating and laying eggs for the adult insects, replacing a culture dish for laying eggs with a new feed culture dish, and taking out and collecting egg masses laid by the adult insects;
thirdly, transferring the collected egg masses into a room for artificial feeding;
1) putting the collected egg masses and the plastic strips into a culture tank for 1-2 days for culture;
2) putting Spodoptera frugiperda breeding feed into the culture tank, and putting the egg mass and the plastic strip above the breeding feed;
3) placing the culture tank into an incubator, setting the culture temperature at 23-26 ℃ and the humidity at 60-65%, and incubating spodoptera frugiperda eggs;
4) feeding after the hatching is finished, wherein the feeding temperature is 25-27 ℃, and the humidity is 60-70%;
5) when the Spodoptera frugiperda pupae becomes hard and dark brown, taking the pupae out of the culture tank, putting the pupae into a plastic box, and performing eclosion in a dark environment;
collecting the cocoons of the Spodoptera frugiperda parasitism bees, and culturing for 12-14 days to enable the cocoons to emerge;
step five, selecting 1-day-old Oncorhynchus Spodoptera frugiperda egg blocks, inoculating parasitic Oncorhynchus Spodoptera bees to the egg blocks, placing the egg blocks in a bee breeding box after inoculating the parasitic Oncorhynchus Spodoptera bees, covering a box cover of the bee breeding box, placing the bee breeding box on a bee breeding frame, and culturing offspring bees;
step six, carrying out parasitic propagation of parasitic wasps to ensure that the parasitic wasps spin cocoons to obtain the cocoons of the parasitic wasps;
and seventhly, storing the cocoons of the parasitic wasps in an environment of 20-23 ℃, and transporting the cocoons to a Spodoptera frugiperda threat area for releasing.
2. The method for expanding propagation of Spodoptera frugiperda parasitic wasps as claimed in claim 1, wherein in the first step, the Spodoptera frugiperda pupae needs to wait for 7-10 days before being lured, and then eclosion of the Spodoptera frugiperda pupae becomes Spodoptera frugiperda imagoes.
3. The method for propagating Spodoptera frugiperda parasitic wasp as claimed in claim 1, wherein in step (1.1), said Spodoptera frugiperda attractant is provided with:
the device comprises a support rod, an attractant shell, a transparent insect collector, an attractant sheet placing groove, an attractant channel and a blocking cover;
the lure device shell is cylindrical and is connected with the support rod through a bolt; the bottom end of the attractant shell is provided with a transparent insect collector, the middle position of the upper end of the attractant shell is provided with an attractant sheet placing groove, and insect attracting channels are arranged around the attractant sheet placing groove; a blocking cover is arranged 1-2cm above the insect attracting passage, and the diameter of the blocking cover is consistent with that of the attractant shell.
4. The method for expanding propagation of Spodoptera frugiperda parasitic wasp as claimed in claim 1, wherein in step (1.1), the attractant piece comprises (Z) -7-dodecen-1-ol acetate 1-2 parts, (Z) -9-tetradecene acetate 1-2 parts, (Z) -11-hexadecene alcohol acetate 2-3 parts, (Z) -9-dodecen-1-ol acetate 1-3 parts, phenylacetaldehyde 300-400 parts, linalool 400-600 parts, dispersant 6-8 parts, slow release agent 10-25 parts, and water 40-50 parts by mass.
5. The method for propagating Spodoptera frugiperda parasitic wasps according to claim 1, wherein in step (1.1), the attractant sheets are prepared by a method comprising the steps of:
(1.1.1) mixing (Z) -7-dodecen-1-ol acetate, (Z) -9-tetradecene acetate, (Z) -11-hexadecene alcohol acetate, (Z) -9-dodecen-1-ol acetate according to corresponding parts by mass to obtain a mixture A;
(1.1.2) mixing phenylacetaldehyde and linalool according to the mass parts to obtain a mixture B;
(1.1.3) adding a dispersing agent into the mixture B, adding water, and uniformly stirring to obtain a mixture C;
(1.1.4) slowly pouring the mixture A into the mixture C, continuously stirring, and adding a slow release agent during stirring to obtain an attractant;
(1.1.5) dropwise adding the attractant into the mold, putting the mold into a drying oven, and drying at the set drying temperature of 50-55 ℃ for 25-35min to obtain the attractant sheet.
6. The method for expanding propagation of Spodoptera frugiperda parasitic wasps as claimed in claim 1, wherein in step (2.1), the temperature of said climatic incubator is 28 ℃, the RH is 78%, and the L: d ═ 16: 8.
7. the method for expanding propagation of Spodoptera frugiperda parasitic wasps as claimed in claim 1, wherein in step 2), the method for preparing the Spodoptera frugiperda feeding feed comprises the following steps:
step a, boiling distilled water, and adding edible agar to completely dissolve the edible agar to obtain agar liquid;
step b, mixing the wheat bran and the corn flour, adding distilled water, and uniformly stirring;
c, adding agar liquid into the mixed liquid, uniformly stirring, standing and cooling;
and d, after the temperature is reduced to below 60 ℃, adding glucose, yeast and vitamin B, and uniformly stirring to obtain the spodoptera frugiperda feeding feed.
8. The method for expanding propagation of Spodoptera frugiperda parasitic wasps as claimed in claim 1, wherein in step four, the Spodoptera frugiperda parasitic wasp is Taiwan Chitosa cocoon bee.
9. The spodoptera frugiperda parasitic wasp propagation method as claimed in claim 1, wherein in the sixth step, the parasitic propagation of the parasitic wasps is specifically carried out as follows: the method comprises the steps of firstly putting cultured Spodoptera frugiperda parasitism bee offspring bees into a breeding box, then putting Spodoptera frugiperda egg masses or larvae into the breeding box, then placing the breeding box into an artificial climate box with the temperature set to be 25 ℃ and the relative humidity of 70%, feeding the breeding box until the Spodoptera frugiperda parasitism bees bite into mature larvae of Spodoptera frugiperda, and then spinning cocoons to obtain the bee cocoons.
10. The propagation method of Spodoptera frugiperda parasitic wasps as claimed in claim 1, wherein in the sixth step, during the parasitic propagation of the parasitic wasps, 15% of honey water is adsorbed by cotton or sponge to provide nutrition for the seed wasps, the temperature in the propagation box is controlled to be 23-26 ℃, the relative humidity is kept at 60-70%, and ventilation is kept.
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