CN102017929B - Method for artificially feeding ladybirds or lacewings by using lepidoptera insect larvae - Google Patents
Method for artificially feeding ladybirds or lacewings by using lepidoptera insect larvae Download PDFInfo
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- CN102017929B CN102017929B CN2009100924899A CN200910092489A CN102017929B CN 102017929 B CN102017929 B CN 102017929B CN 2009100924899 A CN2009100924899 A CN 2009100924899A CN 200910092489 A CN200910092489 A CN 200910092489A CN 102017929 B CN102017929 B CN 102017929B
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- 241000255777 Lepidoptera Species 0.000 title claims abstract description 10
- 241000258923 Neuroptera Species 0.000 title abstract 7
- 235000013601 eggs Nutrition 0.000 claims abstract description 32
- 241001064674 Chrysopa septempunctata Species 0.000 claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 16
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- 229920001817 Agar Polymers 0.000 claims description 15
- 239000008272 agar Substances 0.000 claims description 15
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 14
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 13
- 240000003768 Solanum lycopersicum Species 0.000 claims description 13
- 230000001419 dependent effect Effects 0.000 claims description 11
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- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 9
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- 235000010241 potassium sorbate Nutrition 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
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- 108010000912 Egg Proteins Proteins 0.000 claims description 7
- 229930003268 Vitamin C Natural products 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 7
- CHHHXKFHOYLYRE-STWYSWDKSA-M potassium sorbate Chemical class [K+].C\C=C\C=C\C([O-])=O CHHHXKFHOYLYRE-STWYSWDKSA-M 0.000 claims description 7
- 235000019154 vitamin C Nutrition 0.000 claims description 7
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention provides a method for artificially feeding ladybirds and/or lacewings using lepidoptera insect larva. The method comprises the following steps of: (1) incubating lepidoptera insect larvae after lepidoptera insect adults lay eggs, and feeding the lepidoptera insect larvae with feed; (2) feeding ladybird larvae and/or lacewing larvae by using the lepidoptera insect larvae as feed to obtain ladybird adults and/or lacewing adults, wherein the lacewings are selected from one of chrysopa septempunctata and chrysoperla nipponensis; and (3) feeding the ladybird adults and/or chrysopa septempunctata adults by using the lepidoptera insect larvae as the feed, and feeding chrysoperla nipponensis adults by using honey and beer dry yeast powder. Compared with the prior art, the method for feeding the ladybirds and the lacewings in a large scale by using the lepidoptera insect larvae as the feed replaces the conventional method for feeding the ladybirds and the lacewings which are natural enemies of aphids by using the aphids cultured by plants, and the method saves more time and labor, is simpler and more convenient and is easier to operate.
Description
Technical field
The present invention relates to extermination of disease and insect pest field.The present invention relates to a kind of lepidopterous larvae that adopts is the method that feed is raised ladybug or Chrysopa.
Background technology
Ladybug and Chrysopa are important predator insects, be mainly used in anti-eliminate aphis, mite class, scale insect, aleyrodid etc. relatively are difficult to the agriculture and forestry injurious insect prevented and treated.At present, the heavy industrialization breeding technology of ladybug or Chrysopa also is short of very much, still is in the very elementary stage, and its key reason is to prepare the technological immature of artificial feed.And adopt natural feed such as aphid to carry out raising need plantation plant, and on plant, inoculate aphid, treat that aphid breeds certain density after, offer the predation of ladybug or Chrysopa again.Like this, feed and cost of labor are all very high, and need the plantation and the growing space of bigger aphid host plant.In addition, killing each other etc. of ladybug, Chrysopa also limited the large-scale industrialized production of ladybug and Chrysopa, becomes the limiting factor that a large amount of natural enemy insects are provided with low cost.
Summary of the invention
Therefore, the method that the object of the present invention is to provide a kind of mass artificial simple and easy to do, with low cost to raise ladybug and/or Chrysopa; And the method for using it for the biological control of agriculture and forestry injurious insect.
On the one hand, the invention provides the method for a kind of artificial feeding ladybug and/or Chrysopa, for example, the method for a kind of artificial feeding ladybug or chrysopa septempunctata (Chrysopa septempunctata), this method may further comprise the steps:
(1) hatches the lepidopterous insects larva after the lepidopterous insects adult lays eggs; Adopt the feed lepidopterous insects larva of feeding, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water;
(2) obtain grandis adults or chrysopa septempunctata adult with feed ladybug larva or chrysopa septempunctata larva of lepidopterous insects larva; And
(3) with lepidopterous insects larva feed grandis adults or chrysopa septempunctata adult, after laying eggs, adult hatches ladybug larva or chrysopa septempunctata larva.
Preferably, said step (3) may further comprise the steps:
I) under 25～27 ℃, relative moisture are 60～80% condition, with 2 age feed grandis adults or chrysopa septempunctata adult of lepidopterous insects larva, treat that adult lays eggs after 10～20 days;
II) collect egg hatch and go out ladybug larva or chrysopa septempunctata larva after, be to feed with the lepidopterous insects larva that just incubates under 60～80% the condition at 25～27 ℃, relative moisture with it; And
III) 3 or 4 age the ladybug larva or the chrysopa septempunctata larva with 2 age the lepidopterous insects larva feed.
Again for example, the method for a kind of artificial feeding expert on Japan Chrysopa (Chrysopa nipponensis), this method may further comprise the steps:
1) hatches the lepidopterous insects larva after the lepidopterous insects adult lays eggs; Adopt the feed lepidopterous insects larva of feeding, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water;
2) obtain expert on Japan Chrysopa adult with the lepidopterous insects larva expert on Japan Chrysopa larva of feeding; And
3) with honey and the beer dried yeast powder expert on Japan Chrysopa adult of feeding, after laying eggs, adult hatches expert on Japan Chrysopa larva.
Preferably, said step 3) may further comprise the steps:
I) under 25～27 ℃, relative moisture are 60～80% condition,, treat that adult lays eggs after 10～20 days with honey and the beer dried yeast powder expert on Japan Chrysopa adult of feeding;
After ii) collecting egg hatch and going out expert on Japan Chrysopa larva, be to raise under 60～80% the condition to feed at 25～27 ℃, relative moisture with the lepidopterous insects larva that just incubates with it; And
Iii) 3 or 4 age expert on Japan Chrysopa larva with 2 age the lepidopterous insects larva feed.
Preferably; In said step (1); With feed and the hydromel lepidopterous insects adult of feeding, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water; The volumetric concentration of hydromel is 5～20%, is preferably 10%.
Preferably; In said step (2); The ladybug larva that just incubates, chrysopa septempunctata larva or expert on Japan Chrysopa larva adopt the lepidopterous insects larva that just incubates to feed, 3 or 4 age ladybug larva, chrysopa septempunctata larva or expert on Japan Chrysopa larva adopt 2 age the lepidopterous insects larva feed.
Preferably, said lepidopterous insects is a beet armyworm.
Further preferably, said method comprising the steps of:
A. the raising of lepidopterous insects
Adopt the feed lepidopterous larvae of feeding; And adopt this feed and the hydromel lepidoptera imago of feeding; Treat to hatch larva after this adult lays eggs; Adopt this feed larva of feeding once more, grow up to adult after pupating, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water; The volumetric concentration of said hydromel is 5～20%, is preferably 10%.
B. the raising of ladybug, chrysopa septempunctata or expert on Japan Chrysopa
A. imago breeding: at 25～27 ℃; Under 60～80% the relative moisture condition, in dependent insect cage, raise adult, the adult of ladybug or chrysopa septempunctata add every day 2 age lepidopterous larvae; With the feed adult of expert on Japan Chrysopa of honey and beer dried yeast powder, treat to lay eggs after 10～20 days;
B. the collection of ovum and preservation: adopt egg holder or paper slip to put into the adult dependent insect cage, adult lays eggs on the groove or paper slip of egg holder, changes egg holder or paper slip, collects ovum it is stored in 10～12 ℃ of incubators perhaps directly 25～27 ℃ of hatchings;
C. the raising of larva: culture dish or jar in raise newly hatched larvae, just to incubate the feed larva of ladybug, chrysopa septempunctata or expert on Japan Chrysopa of lepidopterous insects larva; Advanced age ladybug, chrysopa septempunctata or expert on Japan Chrysopa larva transfer in the breathable plastic box with 2 age the lepidopterous insects larva feed 20～40 lepidopterous larvaes of feeding every every day;
D. the processing of pupa: after treating that it pupates, beat easily down and put into culture dish, concentrate and put into the adult dependent insect cage, raise the back of sprouting wings; And
E. preservation of adult and release: store adult down at 12 ℃, the added body volume concentrations is 10% hydromel weekly, and storage life 3～5 months is treated to carry out when the field needs the field and discharged.
On the other hand, the present invention also provides a kind of method of pest control, and this method comprises that the ladybug, chrysopa septempunctata or the expert on Japan Chrysopa that utilize the method for the invention to raise are discharged into the field and/or the woods.
Preferably, said insect is selected from one or more in aphid, mite class, scale insect and the aleyrodid.
Compare with conventional art, method of the present invention is that feed is raised ladybug or Chrysopa on a large scale with the lepidopterous insects larva, the way that has replaced traditional aphid to raise natural enemy insect ladybug, Chrysopa with the plant cultivation, and this method is more time saving and energy saving, simple and easy to do.
Embodiment
The concrete embodiment of following reference explains the present invention.It will be appreciated by those skilled in the art that these embodiment only are used to explain the object of the invention, the scope that it does not limit the present invention in any way.
Embodiment
1. the raising of lepidopterous insects larva
Adopt the artificial feed prescription of inventor's invention, the prescription of said feed comprises by weight: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water;
Its concrete preparation steps is following:
A. 20 parts tomato is broken into pasty state, add 200 parts water, the agar powder of 2 parts of weight and rapeseed oil, the limit is stirred and heated to boiling, and agar is dissolved fully;
B. in addition load weighted casein and wheat germ powder potassium sorbate vitamin etc. is put into a container mixing together; With
C. dissolving in the steps A is good tomato agar mixture is poured in the container of B while hot, and the material mixing among the step B in the tomato agar mixture, is solid mixture after the cooling, cuts apart to store the lepidopterous larvae of feeding again.
Raise beet armyworm with these artificial feed, larva is on average gone through 14 days phases, and average pupa weighs 0.105 gram (not finding the population degradation phenomena more than 32 generations) beet armyworm of feeding; The beet armyworm adult feed above-mentioned feed in addition volumetric concentration be 10% hydromel, after adult lays eggs, ovum collecting is sterilized; Wait to hatch, be transferred to adding artificial feed raising in the culture dish after the hatching, along with the constantly additional artificial feed of the growth of larva; Treat to change in the square box after it got into for 4 ages and raise; After pupating, sterilization is dried, and changes mate and oviposit to be sprouted wings in the adult dependent insect cage again over to.
2. the raising of grandis adults
In the dependent insect cage that lucite is processed, raise ladybug,
Adopt the beet exigua larvae of 2 age Mos to raise; At 25～27 ℃, under the condition of relative moisture 60～80% with imago breeding in dependent insect cage, the in addition sufficient beet exigua larvae every day artificial feed of beet exigua larvae in addition; Beet exigua larvae can continue to get the food growth; Every ladybug is approximately got the food beet exigua larvae of 20 2 age Mos, and adult eclosion mate and oviposit after one week is laid eggs after 10～20 days in a large number.
3. the store collected of ovum
In the ladybug dependent insect cage, put into egg holder or paper slip, ladybug is liked laying eggs at the groove of egg holder, often changes the egg holder, and ladybug is preferred in fresh egg holder, laying eggs.Adult lays eggs on egg holder or paper slip, after the collection, will directly hatch and raise or in storing 10 days under 8～12 ℃, take out when needing and hatch.Collect a large amount of ovum and can concentrate extensive raising.
4. the raising of larva
The airtight raising in culture dish or plastic tank of low age ladybug larva; Add the beet exigua larvae and the feed thereof of just incubating; This feed is raised lepidopterous larvae for the artificial feed of inventor's invention; Purpose is for letting beet exigua larvae get anthropophagy worker's feed, and the length of side is eaten on the limit, makes it that superior food environment arranged; Obtain more sufficient lepidopterous larvae as food so that predator is better, the prescription of said artificial feed of the present invention comprises by weight: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water;
Its concrete preparation steps is following:
A. 20 parts tomato is broken into pasty state, add 200 parts water, the agar powder of 2 parts of weight and rapeseed oil, the limit is stirred and heated to boiling, and agar is dissolved fully;
B. in addition load weighted casein and wheat germ powder potassium sorbate vitamin etc. is put into a container mixing together; With
C. dissolving in the steps A is good tomato agar mixture is poured in the container of B while hot, and the material mixing among the step B in the tomato agar mixture, is solid mixture after the cooling, cuts apart to store the lepidopterous larvae of feeding again.
The larva of every ladybug can add the beet armyworm newly hatched larvae more than 20, and 3 days can be without supplement feed.The ladybug larva was raised two days again at the beginning of replenishing 1 age Mo or 2 ages after 3 days; Ladybug got at the beginning of 3 age Mos or 4 ages; Change in the big plastics square box and raise, increase the space, add again 2 age beet exigua larvae; Predation every every day can reach 30～40 2 age beet exigua larvae, can change additional beet exigua larvae in per two days.Basically larva raising just can be pupated in 8～10 days.
3, the ladybug larva in 4 ages is raised in big plastic casing, and plastic casing covers with gauze ventilated, raises with the beet exigua larvae of 2 age Mos.As long as the generation of seldom killing each other of sufficient feed and space is arranged.
5. the management of pupa
The ladybug larvae pupation is beaten easily down two days later, puts into culture dish, puts into the dependent insect cage of adult again and treats directly to raise with the beet exigua larvae after pupa is sprouted wings.
6. the storage of adult
Under 12 ℃, the adult time-to-live of ladybug can reach 3-5 month, and is middle whenever at a distance from additional 10% hydromel of 2 weeks.
One week of the adult eclosion back mate and oviposit of ladybug is laid eggs after 20 days in a large number.At 25～27 ℃, under the condition of relative moisture 60～80%, to hatch 3 days, incubation rate is 72.4%, 8～10 days larval phases,, pupation rate reaches 50～80%, 4～5 days pupa time.Eclosion rate 78%, adult survival 3～5 months.
Claims (2)
1. the method for an artificial feeding ladybug, chrysopa septempunctata or expert on Japan Chrysopa, this method may further comprise the steps:
A. the raising of lepidopterous insects
Adopt the feed lepidopterous larvae of feeding; And adopt this feed and the hydromel lepidoptera imago of feeding; Treat to hatch larva after this adult lays eggs; Adopt this feed larva of feeding once more, grow up to adult after pupating, this feed is counted by weight and is comprised: 200 parts in 10 parts of casein, 20 parts in wheat germ powder, 20 parts of tomatoes, 3 parts of potassium sorbates, 0.2 part of vitamin C, 0.7 part of rapeseed oil, 2 parts in agar and water; The volumetric concentration of hydromel is 5～20%;
B. the raising of ladybug, chrysopa septempunctata or expert on Japan Chrysopa
A. imago breeding: at 25～27 ℃; Under 60～80% the relative moisture condition, in dependent insect cage, raise adult, the adult of ladybug or chrysopa septempunctata add every day 2 age lepidopterous larvae feed; With honey and the beer dried yeast powder expert on Japan Chrysopa of feeding, treat to lay eggs after 10～20 days;
B. the collection of ovum and preservation: adopt egg holder or paper slip to put into the adult dependent insect cage, adult lays eggs on the groove or paper slip of egg holder, changes egg holder or paper slip, collects ovum it is stored in 10～12 ℃ of incubators perhaps directly 25～27 ℃ of hatchings;
C. the raising of larva: in culture dish or jar, raise newly hatched larvae; Just to incubate the feed larva of ladybug, chrysopa septempunctata or expert on Japan Chrysopa of lepidopterous insects larva; The ladybug at advanced age, chrysopa septempunctata or expert on Japan Chrysopa larva transfer to feed in the breathable plastic box 2 age the lepidopterous insects larva, 20～40 lepidopterous larvaes of feeding every every day;
D. the processing of pupa: after treating that it pupates, beat easily down and put into culture dish, concentrate and put into the adult dependent insect cage, raise the back of sprouting wings; And
E. preservation of adult and release: store adult down at 12 ℃, the added body volume concentrations is 10% hydromel weekly, and storage life 3～5 months is treated to carry out when the field needs the field and discharged;
Wherein, said lepidopterous insects is a beet armyworm.
2. method according to claim 1 is characterized in that, in steps A, the volumetric concentration of said hydromel is 10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100924899A CN102017929B (en) | 2009-09-10 | 2009-09-10 | Method for artificially feeding ladybirds or lacewings by using lepidoptera insect larvae |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100924899A CN102017929B (en) | 2009-09-10 | 2009-09-10 | Method for artificially feeding ladybirds or lacewings by using lepidoptera insect larvae |
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| CN102017929A CN102017929A (en) | 2011-04-20 |
| CN102017929B true CN102017929B (en) | 2012-11-07 |
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