CN1631127A - Artificial progagation method for ladybird beetle and lacewing fly - Google Patents
Artificial progagation method for ladybird beetle and lacewing fly Download PDFInfo
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- CN1631127A CN1631127A CN 200510002749 CN200510002749A CN1631127A CN 1631127 A CN1631127 A CN 1631127A CN 200510002749 CN200510002749 CN 200510002749 CN 200510002749 A CN200510002749 A CN 200510002749A CN 1631127 A CN1631127 A CN 1631127A
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Abstract
The invention provides an artificial propagation method for ladybird beetle and lacewing fly which comprises the steps of, aphid culturing, using wheat, lima bean, cabbage, radish as host plant for aphid culturing, and ladybug lacewing fly raising, ovum period management, larva nourishing, chrysalides growth, product refrigeration, and packaging.
Description
Technical field
The present invention relates to a kind of propagation production method of natural enemy insect, particularly a kind of ladybug, Chrysopa artificial propagation production method belong to the biological control technical fields of pest.
Background technology
Ladybug is of a great variety, and mostly is the natural enemy of agriculture and forestry injurious insect, protects and utilize its Pest Control to cause that already people pay attention to, and many successful examples are at home and abroad all arranged.In recent years, the research of aspects such as artificial feed of relevant ladybug and relevant ecology, biological characteristic makes significant progress.But it is perfect that its large-scale breeding technology is still waiting, and all has big problem at aspects such as stocking density and breeding facility, production in enormous quantities technological standards, packing and storing technology, can't produce in a large number, can not satisfy the needs of present production.
Summary of the invention
Technical problem to be solved by this invention is to avoid above-mentioned deficiency of the prior art, and a kind of ladybug, Chrysopa artificial propagation production method are proposed, this method stocking density, temperature and humidity conditions and breeding facility, packing and storing technological sciences, reasonable, spy has realized high-quality, has bred ladybug, Chrysopa efficiently, in enormous quantities, meets the needs of production.
Technical scheme provided by the present invention is: a kind of ladybug, Chrysopa artificial propagation production method may further comprise the steps:
(1) aphid is raised
Raise aphid with wheat, broad bean, Chinese cabbage, radish as the aphid host plant;
(2) ladybug, Chrysopa are cultivated
A. imago breeding: at 25-27 ℃, RH70-85% under 14 hours conditions of illumination, adopts the boxlike method for breeding, the aphid in the step (1) is together put into together with a small amount of plant leaf raise box, and every ladybug, the Chrysopa adult aphid amount of throwing something and feeding is 50-80 head/sky;
B. ovum period management: ladybug, the Chrysopa back of sprouting wings began to lay eggs in 5-7 days, in raising box, place to lay eggs and lure the collection thing, and spraying is laid eggs and is lured liquid collecting, be convenient to collect so that it lays eggs to concentrate, in time collect ladybug, Chrysopa ovum grain, the ovum that collect every day is made the ovum card, and refrigeration is under 8-12 ℃ condition, be one batch in every 5-10 days, the ovum card is taken out in batches, and carry out after the surface sterilization standby;
C. larva is raised scattered: 22-27 ℃, in the insectary of RH75-85%, in cylinder mould, insert and inoculated 3 days host plant seedling-cultivating tray of aphid, then same batch ladybug ovum is inserted uniformly, density is 3 ovum of every strain, and after its hatching, the quantity of aphid also increases gradually on the plant, keep plant grew well this moment, so that aphid keeps enough population quantities;
D. pupa time: 25-27 ℃, under the RH70-85% condition, ladybug, Chrysopa larva 3-4 place during age to lure in raising cylinder mould and collect the device of pupating, it is pupated within it to lure collection, or in the larva maturing stage, divides plant leaf together to collect in the cultivation container its company headquarters and pupate, in the Chrysopa pupa enters after pupa time, can preserve under the RH55-75% condition with it at 2-5 ℃, storage life was 50 days;
E. the emergence of adult: ladybug, Chrysopa pupa are put into 22-27 ℃, grow under the condition of RH70-85%, raise after the emergence, female-male proportion is 3-5 during raising: 1, ladybug is raised a Zhou Houke in 7-10 ℃, refrigerate under the RH55-75% condition, during the hydromel of feeding in every 15-20 days supplement the nutrients, storage life was 100 days;
(3) packing: adult is box-packaged, larva, ovum paper bag type packing.
Further, describedly raise aphid with wheat, broad bean, Chinese cabbage, radish as the aphid host plant, at temperature 18-27 ℃, under 14 hours conditions of illumination, adopting has soil or does not have a cubic metre of earth formula cultivation host plant.
Further, describedly raise aphid as the aphid host plant with wheat, Chinese cabbage, radish, when wheat, Chinese cabbage, radish height of seedling during to 2-3 centimetre, the corresponding access wheat aphid of ratio, black peach aphid, vegetable aphid in 2-5 aphid of every strain plant, when its growth every strain in 5-7 days has aphid 15-20, can provide live body feed as the predator larva.
Further, describedly raise aphid as the aphid host plant with broad bean, when broad bean height of seedling during to 3-5 centimetre, inoculation black bean aphid, bean aphid or black peach aphid, inoculative proportion is 3-5 head/strain, after 6-7 days, individual plant aphid amount 50-70 head, after 10-15 days, every strain broad bean seedling can be bred aphid 100 bulls, every basin can obtain 15000 of aphids, and the live body feed as the predator larva can be provided.
The present invention has following advantage: host plant kind and the aphid of selecting efficient breeding host aphid obtain technology in a large number; Ladybug, Chrysopa were raised by its ovum, larva, pupa and four developmental stage of adult by stages, adopted suitable breeding technology according to its biology, ecological characteristics; Development test a large amount of breeding utensils, improved reproductive efficiency; Utilize that physics and chemical method lure the collection ladybug, Chrysopa lays eggs and pupate, be convenient to centralized collection and cultivate; The ovum of making is sticked into capable surface sterilization, improve its incubation rate; Raising and condition of storage have been determined in test; Adult is box-packaged, and larva, ovum paper bag type packing is convenient to the field and is discharged and apply.Explore the key technology of utilizing the live body aphid to breed ladybug, Chrysopa in enormous quantities, realized high-quality, bred ladybug, Chrysopa efficiently, in enormous quantities, be used for anti-agriculture and forestry injurious insect such as eliminate aphis, meet the needs of production.
Description of drawings
Fig. 1 is ladybug of the present invention, Chrysopa artificial propagation production method flow chart.
Embodiment
Embodiment 1: ladybug, Chrysopa are produced in artificial propagation
Referring to accompanying drawing 1, the step that ladybug, Chrysopa are produced in artificial propagation is as follows:
1, aphid cultivating
With wheat as the aphid host plant, 20 ℃ of temperature, under 14 hours conditions of illumination, adopt soilless culture mode cultivated wheat, cushioning absorbent wool and make maintenance moisture at the bottom of 20*30 centimetre seedling-cultivating tray, upper berth old newspaper then, on newspaper, broadcast sowing wheat seed, about 1000 on every dish is broadcast back loam cake plastic film and is preserved moisture, and opens after emerging, when treating that wheat seeding grows to 2-3 centimetre high, ratio in 4 aphids of every strain inserts wheat aphid, cultivates and grows when every strain wheat seeding has aphid 15-20 after 6 days, and the live body feed as the predator larva can be provided.
The method that broad bean is cultivated aphid is: 22 ℃ of temperature, under 14 hours conditions of illumination, planted a collection of broad bean in 10 days, after every batch of 20 baskets of presproutings of seeds, sowing is in 20*30 centimetre plastic crate, and every basket 150 left and right sides grain germination seed is broadcast 1 centimetre of back earthing, the loam cake Polypropylence Sheet is preserved moisture, and opens Polypropylence Sheet after emerging.When the broad bean seedling grows to 3-5 centimetre high, can inoculate bean aphid or black peach aphid, inoculative proportion is 4/strain.After 7 days, individual plant aphid amount 50-70 head; After 12 days, every strain broad bean seedling can be bred aphid 100 bulls.Every basin can obtain 15000 of aphids.
The method that crucifer such as Chinese cabbage, radish is cultivated aphid is: 25 ℃ of temperature, under 13 hours conditions of illumination, planted crucifers such as a collection of Chinese cabbage, radish, every kind of each 40 basin, every basin one strain in per 10 days.When growing to 4-6 sheet leaf, every basin connects 100 of black peach aphids, and after 13 days, every basin can be bred the over thousands of head of aphid.
2, ladybug, Chrysopa are cultivated
(1) imago breeding: at 26 ℃, RH75%, under 14 hours conditions of illumination, adopt the boxlike method for breeding, aphid in the step (1) is together put into the raising box together with a small amount of plant leaf, every ladybug, the Chrysopa adult aphid amount of throwing something and feeding is 70/day, artificial eggs cute conjunctivitis bee pupa can be when aphid lacks as the supplement feed of grandis adults.
The emergence of adult: ladybug, Chrysopa pupa are put into 25 ℃, grow under the condition of RH75%, raise after the emergence, female-male proportion is 4: 1 during raising, and ladybug is raised all backs in 8 ℃ of temperature, refrigerates under the RH60% condition;
(2) ovum period management: ladybug, the Chrysopa back of sprouting wings began to lay eggs in 5-7 days, place to lay eggs to lure the collection thing in raising box, and spraying laid eggs and lure liquid collecting, was convenient to collect so that it lays eggs to concentrate.In time collect ladybug, Chrysopa ovum grain, the ovum of collecting every day is made the ovum card, is one batch in per 7 days, the ovum card is taken out in batches, and carry out after the surface sterilization standby;
(3) larva is raised scattered: 25 ℃, in the insectary of 75-85%, in cylinder mould, insert and inoculated 3 days host plant seedling-cultivating tray of aphid, then same batch ladybug ovum is inserted uniformly, density is 3 ovum of every strain, and after its hatching, the quantity of aphid also increases gradually on the plant, will keep plant grew well this moment, so that aphid keeps enough population quantities; In addition, artificial eggs cute conjunctivitis bee pupa can substitute aphid as the ladybug larvae feeds.
(4) pupa time: 25 ℃, under the RH75% condition, ladybug, Chrysopa larva 3-4 place to lure in raising cylinder mould during age and collect the device of pupating, and it is pupated within it to lure collection, or in the larva maturing stage, divides plant leaf together to collect in the cultivation container its company headquarters and pupate.
3, the refrigeration of product:
(1) during the Chrysopa pupa enters after pupa time, it can be preserved under 2-5 ℃, RH55-75% condition, still can normally sprout wings in 50 days;
(2) under 7-10 ℃, RH55-75% condition, refrigerate after ladybug emergence 7-10 days, during the every 15-20 days hydromels of feeding supplement the nutrients, storage life can reach about 100 days;
4, packing: the box-packaged release of adult is used, larva, ovum paper bag type packing.
Test implements 1: feed to the influence of ladybug larva lethality in each and adult pick-up rate in the length of time
For the examination borer population
Lethality (%)Adult obtains
Feed is handled
(head) ages two pupa rates in four ages in three ages in age (%)
Artificial eggs cute conjunctivitis bee pupa 30 00000 100a
Nature ovum cute conjunctivitis bee pupa 30 3.33 0 3.33 00 93.33b
Drone pupae 30 3.33 0 3.33 3.33 0 90b
Bean aphid 30 00000 100a
Annotate: different lowercase alphabet differential different significantly (P＜0.05) in the table (down with)
The feed pick-up rate of adult and pupa of artificial eggs cute conjunctivitis bee pupa and bean aphid is best, and difference is not remarkable, but handles significant difference with natural ovum cute conjunctivitis bee pupa and male peak pupa; It is not remarkable that nature ovum cute conjunctivitis bee pupa and male peak pupa are handled differences.
Experimental example 2: feed is gone through the phase to the ladybug larvae development and is observed
For the examination borer population
Development duration (my god)
Feed is handled
(head) age four-age larva phase in three ages two ages pupa
Artificial eggs cute conjunctivitis bee pupa 30 2.80 ± 0.15A 2.10 ± 0.21A 1.43 ± 0.25A 3.87 ± 0.27A 10.17 ± 0.36Aa 5.63 ± 0.55A
Nature ovum cute conjunctivitis bee pupa 28 3.14 ± 0.14B 1.93 ± 0.10B 2.82 ± 0.18B 4.36 ± 0.24B 12.25 ± 0.33B 6.57 ± 0.29B
Drone pupae 27 2.21 ± 0.15C 2.21 ± 0.21C 2.93 ± 0.15B 4.26 ± 0.18B 11.67 ± 0.29C 7.59 ± 0.28C
Bean aphid 30 2.13 ± 0.13C 1.90 ± 0.11B 2.00 ± 0.10A 3.73 ± 0.20A 9.73 ± 0.26Ab 6.03 ± 0.16D
Annotate: different capitalizations are represented difference extremely significantly (P＜0.01) in the table
Artificial eggs cute conjunctivitis bee pupa is raised and is fed bean aphid, and it is the shortest that the ladybug larvae development is gone through the phase, and both do not have significant difference; Nature ovum cute conjunctivitis bee pupa and male peak pupa breeding grub do not have difference between handling, but compare significant difference with preceding two kinds of processing.
Experimental example 3: the ladybug not same sex is compared than egg laying amount
The ladybug mating is once laid eggs all the life, but the existence of male worm stimulates female worm to lay eggs again.Therefore in large-scale breeding, explore the optimality ratio of group breeding grandis adults, significant to improving reproductive efficiency.
If 4 individual character other places reason: female: male=1: 1; Female: male=2: 1; Female: male=5: 1; Female: male=18: 0 (female worm mating at least crosses once).Each processing ladybug after differentiating male and female is put into the plastics of 12*7*16cm and raises box, 18 in every box.Supply with the fresh broad bean blade that has aphid of capacity.Clean the swill broad bean that here withers every day, replenish aphid, and collect record and respectively handle the number of eggs laid amount, repeat 3 times.Test is at T=25 ℃, RH=75, L: D=14: carry out in 10 the climatic cabinate, result of the test sees the following form.
| Handle | Total egg laying amount/sky | Single female egg laying amount/sky | Incubation rate (%) |
| Female: male=1: 1 | ????915.58±147.23a | ????33.91±5.74a | ????81.64±0.09a |
| Female: male=2: 1 | ????1143.32±142.89ab | ????31.76±3.97a | ????73.83±23.31a |
| Female: male=5: 1 | ????1535.53±220.80b | ????34.12±4.91a | ????90.80±19.52a |
| Female: male=18: 0 | ????1279.47±199.14c | ????23.69±3.69b | ????87.02±6.60a |
It can be seen from the table, the significant stimulation egg laying amount that exists of male worm increases (comprising total egg laying amount and every female egg laying amount), but 3 different property are more little than the average egg laying amount difference of every female worm every day.Total egg laying amount maximum that identical cultured space and food is obtained when the property ratio is 5: 1.The same sex is more not remarkable than the egg hatching rate difference that the ladybug of handling gives birth to.
Experimental example 4: the influence that feed grows to Chrysopa
For the examination feed be: (weight proportion is A. brewer's yeast dry powder: beer yeast powder 10; Sucrose 8, other adds trace protein); B. (weight proportion is the tussah chrysalis freeze-dried powder: freeze-dried powder 5; Honey 2; Sucrose 2); C. artificial eggs cute conjunctivitis bee pupa; D. rice moth egg; The CK bean aphid.
With the bean aphid live body worm cocoon chrysopa septempunctata adult that newly sprouts wings that survives the winter of feeding, when laying eggs, place glass to raise tube on its ovum, all seal with the individual layer cotton at the glass infuser two ends, be placed in 25 ± 1 ℃ of temperature then, relative moisture 60%-70%, L: D=16: grow in the climatic cabinate under 8 conditions, hatch larva and be for experiment.
1, feed is to the influence of Chrysopa development duration
Test is established 5 processing according to DIFFERENT FEED, 3 repetitions.Newly hatched larvae is respectively charged into to raise in the tube and raises, and every 10, at the bottom of bottle, put into the shredded paper bar as spacer assembly, prevent to cut one another's throat.The 5 kinds of feeds of feeding respectively every day, and regularly change, moisture supplied with sponge ball.Every day observed and recorded larvae development progress, until pupate, emergence, death.
The result shows: brewer's yeast dry powder, 2 kinds of artificial feed of the tussah chrysalis freeze-dried powder chrysopa septempunctata newly hatched larvae of feeding, larva is dead successively between a length of time.Raise chrysopa septempunctata with 3 kinds of DIFFERENT FEED of artificial eggs cute conjunctivitis bee pupa, rice moth egg, aphid, to chrysopa septempunctata larva, pupa, each worm attitude development duration of adult and the preoviposition period and the influence of the phase of going through of laying eggs see the following form.
DIFFERENT FEED to the influence of chrysopa septempunctata development duration (my god)
Feed/go through adult life-span pupa time larval phase phase lays eggs the preoviposition period of going through the phase
Cute conjunctivitis bee pupa 8.61 ± 0.29A 14.05 ± 0.25A 27.3 ± 5.21a 7.33 ± 3.54 18.67 ± 3.3
Rice moth egg 9.42 ± 0.59A 14.82 ± 0.83A 23.00 ± 4.15a //
Aphid 6.41 ± 0.00B 10.86 ± 0.13B 31.93 ± 3.57a 25.50 ± 3.80 9.0 ± 0.51
* the phase is gone through in " mean+SD " expression, the large and small different significant differences of representing to have p=0.01 and p=0.05 level respectively of letter of writing of same column
Cute conjunctivitis bee pupa and rice moth egg are fed for feed for two kinds, to the development duration no significant difference of chrysopa septempunctata larva, pupa.But all the control treatment difference of feeding with aphid is extremely remarkable, prolongs 1.8-3 days respectively.And the growth regularity descends.The Chrysopa adult life-span (27.3 days) with the raising of cute conjunctivitis bee pupa is omited weak point than the adult life-span of feeding with aphid, and is longer than omiting with the Chrysopa adult life-span of rice moth egg raising, but equal no significant difference.The adult of raising with rice moth egg does not lay eggs.The significant prolongation preoviposition period of the Chrysopa adult raised than bean aphid with the cute conjunctivitis bee pupa, about 2 times of raising for bean aphid, and the phase of going through of laying eggs is starkly lower than the chrysopa septempunctata that aphid is raised, only be its about 1/3.
2, feed is to the Chrysopa survival rate (rate of cocooing; Eclosion rate; The adult survival rate) influence
Test is established 5 processing according to DIFFERENT FEED, puts into 10 newly hatched larvaes respectively for every bottle, repeats 3 times.Observed each worm attitude development progress every day at interval in 8 hours, calculates cocoon rate, eclosion rate, and observe the record adult from sprouting wings to the survival rate of laying eggs, and result of the test sees the following form.
DIFFERENT FEED is to the influence of chrysopa septempunctata survival rate
Feed/survival rate (%) rate eclosion rate adult survival rate of cocooing
Cute conjunctivitis bee pupa 76.7A 69.6A 50
Rice moth egg 63.3A 89.5B 0
Aphid 96.7B 96.6B 96.4
* same column capitalization different table is shown with utmost point significant difference (p=0.01)
Presentation of results, the Chrysopa larva of raising with the artificial eggs cute conjunctivitis bee pupa rate (76.7%) of cocooing is slightly higher than the larva of raising with the rice moth egg rate (63.3%) of cocooing, but no significant difference, both compare with the rate (96.7%) of cocooing with the chrysopa septempunctata of aphid raising, and utmost point significant difference is arranged.The eclosion rate (69.6%) of the chrysopa septempunctata of raising with the cute conjunctivitis bee pupa extremely significantly is lower than the chrysopa septempunctata of raising (89.5%) and aphid raising (96.6%) with rice moth egg.In addition, the Chrysopa adult survival rate of raising with the cute conjunctivitis bee pupa is 50%, with Chrysopa (96.7%) significant difference of contrast with the aphid raising.And the Chrysopa of raising with rice moth egg does not lay eggs.
3, feed is to the Chrysopa egg laying amount and the influence in adult life-span
With the adult male and female pairing of newly sprouting wings, be positioned over to raise in the tube and feed, preserve moisture with sponge ball, when to be laid eggs, collected ovum grain every 8 hours every day, adds up female worm egg laying amount, calculates the adult life-span simultaneously.When 3 kinds of feeds were raised the chrysopa septempunctata adult, egg laying amount was widely different.The adult of raising with rice moth egg does not lay eggs, and the Chrysopa egg laying amount of raising with artificial eggs cute conjunctivitis bee pupa is few, and 77.67 of average out to are up to 293, and minimum is 2; With the adult egg laying amount that aphid is raised, 382.69 of average out to are up to 780, and minimum is 10.With the average egg laying amount of adult that the cute conjunctivitis bee pupa is raised, the highest egg laying amount, minimum egg laying amount all are starkly lower than the adult egg laying amount of raising with aphid.The adult mean lifetime: what raise with the cute conjunctivitis bee pupa is 27.3 days, and what raise with rice moth egg is 23 days, and what raise with aphid is 31.93 days; Slightly shorter with the chrysopa septempunctata mean lifetime that cute conjunctivitis bee pupa and rice moth egg are raised than the Chrysopa of raising with bean aphid, but no significant difference; Chrysopa septempunctata adult MaLS that 3 kinds of feeds are raised and short life difference are very big, and the Chrysopa adult MaLS of raising with the cute conjunctivitis bee pupa is 49 days, with the rice moth egg raising be 37 days, with the aphid raising is 60 days; The short life of the Chrysopa of raising with the cute conjunctivitis bee pupa is 11 days, and what raise with rice moth egg is 10 days, and what raise with aphid is 15 days.
Presentation of results has been tested in this test, and brewer's yeast dry powder and tussah chrysalis freeze-dried powder can not satisfy the growth needs of chrysopa septempunctata larva.Artificial eggs cute conjunctivitis bee pupa and rice moth egg can be used as the chrysopa septempunctata larva for feed, but the effect of feeding is compared with aphid to some extent significantly and to be reduced.
Claims (4)
1, a kind of ladybug, Chrysopa artificial propagation production method may further comprise the steps:
(1) aphid is raised
Raise aphid with wheat, broad bean, Chinese cabbage, radish as the aphid host plant;
(2) ladybug, Chrysopa are cultivated
A. imago breeding: at 25-27 ℃, RH70-85% under 14 hours conditions of illumination, adopts the boxlike method for breeding, the aphid in the step (1) is together put into together with a small amount of plant leaf raise box, and every ladybug, the Chrysopa adult aphid amount of throwing something and feeding is 50-80 head/sky;
B. ovum period management: ladybug, the Chrysopa back of sprouting wings began to lay eggs in 5-7 days, in raising box, place to lay eggs and lure the collection thing, and spraying is laid eggs and is lured liquid collecting, be convenient to collect so that it lays eggs to concentrate, in time collect ladybug, Chrysopa ovum grain, the ovum that collect every day is made the ovum card, and refrigeration is under 8-12 ℃ condition, be one batch in every 5-10 days, the ovum card is taken out in batches, and carry out after the surface sterilization standby;
C. larva is raised scattered: 22-27 ℃, in the insectary of RH75-85%, in cylinder mould, insert and inoculated 3 days host plant seedling-cultivating tray of aphid, then same batch ladybug ovum is inserted uniformly, density is 3 ovum of every strain, and after its hatching, the quantity of aphid also increases gradually on the plant, keep plant grew well this moment, so that aphid keeps enough population quantities;
D. pupa time: 25-27 ℃, under the RH70-85% condition, ladybug, Chrysopa larva 3-4 place during age to lure in raising cylinder mould and collect the device of pupating, it is pupated within it to lure collection, or in the larva maturing stage, divides plant leaf together to collect in the cultivation container its company headquarters and pupate, in the Chrysopa pupa enters after pupa time, can preserve under the RH55-75% condition with it at 2-5 ℃, storage life was 50 days;
E. the emergence of adult: ladybug, Chrysopa pupa are put into 22-27 ℃, grow under the condition of RH70-85%, raise after the emergence, female-male proportion is 3-5 during raising: 1, ladybug is raised a Zhou Houke in 7-10 ℃, refrigerate under the RH55-75% condition, during the hydromel of feeding in every 15-20 days supplement the nutrients, storage life was 100 days;
(3) packing: adult is box-packaged, larva, ovum paper bag type packing.
2, ladybug as claimed in claim 1, Chrysopa artificial progagation, it is characterized in that: describedly raise aphid as the aphid host plant with wheat, broad bean, Chinese cabbage, radish, at temperature 18-27 ℃, under 14 hours conditions of illumination, adopting has soil or does not have a cubic metre of earth formula cultivation host plant.
3, ladybug as claimed in claim 1 or 2, Chrysopa artificial progagation, it is characterized in that: describedly raise aphid as the aphid host plant with wheat, Chinese cabbage, radish, when wheat, Chinese cabbage, radish height of seedling during to 2-3 centimetre, the corresponding access wheat aphid of ratio, black peach aphid, vegetable aphid in 2-5 aphid of every strain plant, when its growth every strain in 5-7 days has aphid 15-20, can provide live body feed as the predator larva.
4, ladybug as claimed in claim 1 or 2, Chrysopa artificial progagation, it is characterized in that: describedly raise aphid as the aphid host plant with broad bean, when broad bean height of seedling during to 3-5 centimetre, inoculation black bean aphid, bean aphid or black peach aphid, inoculative proportion is 3-5 head/strain, after 6-7 days, individual plant aphid amount 50-70 head, after 10-15 days, every strain broad bean seedling can be bred aphid 100 bulls, every basin can obtain 15000 of aphids, and the live body feed as the predator larva can be provided.
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2005
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