CN106472431A - A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum - Google Patents
A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum Download PDFInfo
- Publication number
- CN106472431A CN106472431A CN201610924333.2A CN201610924333A CN106472431A CN 106472431 A CN106472431 A CN 106472431A CN 201610924333 A CN201610924333 A CN 201610924333A CN 106472431 A CN106472431 A CN 106472431A
- Authority
- CN
- China
- Prior art keywords
- plant
- pupa
- bemisia tabaci
- host
- circle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000132498 Serangium japonicum Species 0.000 title claims abstract description 96
- 241000382353 Pupa Species 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 57
- 238000009395 breeding Methods 0.000 title claims description 35
- 241000255749 Coccinellidae Species 0.000 claims abstract description 89
- 238000011161 development Methods 0.000 claims abstract description 19
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 241000254127 Bemisia tabaci Species 0.000 claims description 111
- 241000196324 Embryophyta Species 0.000 claims description 101
- 244000061458 Solanum melongena Species 0.000 claims description 87
- 235000002597 Solanum melongena Nutrition 0.000 claims description 53
- 102000002322 Egg Proteins Human genes 0.000 claims description 25
- 108010000912 Egg Proteins Proteins 0.000 claims description 25
- 230000001488 breeding effect Effects 0.000 claims description 25
- 210000004681 ovum Anatomy 0.000 claims description 25
- 230000017448 oviposition Effects 0.000 claims description 24
- 230000001418 larval effect Effects 0.000 claims description 14
- 241000607479 Yersinia pestis Species 0.000 claims description 12
- 235000013305 food Nutrition 0.000 claims description 12
- 208000015181 infectious disease Diseases 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 11
- 230000002354 daily effect Effects 0.000 claims description 7
- 238000009792 diffusion process Methods 0.000 claims description 6
- 239000003755 preservative agent Substances 0.000 claims description 6
- 230000002335 preservative effect Effects 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 4
- 241001392790 Rodolia cardinalis Species 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 14
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 4
- 235000013601 eggs Nutrition 0.000 description 21
- 229920000742 Cotton Polymers 0.000 description 17
- 241000219146 Gossypium Species 0.000 description 17
- 230000018109 developmental process Effects 0.000 description 17
- 240000008067 Cucumis sativus Species 0.000 description 15
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 15
- 238000012360 testing method Methods 0.000 description 13
- 241000208125 Nicotiana Species 0.000 description 12
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 12
- 239000000843 powder Substances 0.000 description 12
- 241000238631 Hexapoda Species 0.000 description 11
- 244000017020 Ipomoea batatas Species 0.000 description 10
- 235000002678 Ipomoea batatas Nutrition 0.000 description 10
- 230000004083 survival effect Effects 0.000 description 10
- 230000012010 growth Effects 0.000 description 8
- 235000016709 nutrition Nutrition 0.000 description 6
- 230000035764 nutrition Effects 0.000 description 6
- 239000000123 paper Substances 0.000 description 6
- 239000004033 plastic Substances 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000000474 nursing effect Effects 0.000 description 5
- 230000001850 reproductive effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000010998 test method Methods 0.000 description 4
- 241001560086 Pachyrhizus Species 0.000 description 3
- 239000011111 cardboard Substances 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 2
- 241000819999 Nymphes Species 0.000 description 2
- 241001674048 Phthiraptera Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000019504 cigarettes Nutrition 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000032669 eclosion Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 239000011087 paperboard Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 241001503987 Clematis vitalba Species 0.000 description 1
- 206010012335 Dependence Diseases 0.000 description 1
- 101100133466 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) nit-4 gene Proteins 0.000 description 1
- 241000488583 Panonychus ulmi Species 0.000 description 1
- 241001414989 Thysanoptera Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000011096 corrugated fiberboard Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a kind of collection method of Serangium japonicum pupa, including obtaining Ladybird egg, raising children phase ladybug, collect the steps such as ladybug pupa.The inventive method is sustainable to obtain the relatively uniform Ladybird egg of development condition, makes, using the convenient single face corrugated board of material source, circle of pupating, and preparation method is easy, and it is easy that pupa collects operating process；For prior art, it is achieved that Serangium japonicum efficiently, quickly, is in high volume bred, have the advantages that scientific and reasonable, simple, convenient and practical.
Description
Technical field
The invention belongs to insect reproduction field, and in particular to a kind of collection method of Serangium japonicum pupa and Japanese nose angle
The artificial large-scale breeding method of ladybug.
Background technology
The breeding of natural enemy insect and utilization are the important means of the sustainable ecological management of insect, using indoor amount reproduction day
Enemy insect, carries out merchandized handling and application, is that instead of and reduces chemical pesticide and use, environmental protection, protection bio-diversity,
Improve one of main path of agricultural output and quality.
Serangium japonicum is the important local predator of Bemisia tabaci, can prey on tobacco powder nit, each age nymph, also may be used
Predation Bemisia tabaci adults, and grandis adults addiction tobacco powder nit, therefore can efficiently control the growth of Bemisia tabaci quantity,
Be conducive to controlling Bemisia tabaci before wildness causes harm, this is acquired a special sense in biological control.Natural enemy wooden dipper is introduced with external
Worm is compared, and the ladybug has had very strong adaptability to China's South China's geographical environment and weather conditions, and the ladybug catches
Heavy (the day predatory number to tobacco powder nit is up to more than 700), adult circles in the air, transfer ability of creeping is strong, and Life of Adult is long, numerous
Grow power strong, breeding cycle generation is shorter, be that large scale artificial breeding and release are applied there is provided possible.
Systematic research be there is no in terms of Serangium japonicum indoor feeding at present, be mostly with gather field Japan nose angle
Based on ladybug, and raised for completing experiment in a short time indoors, lack and indoor long-term, Steady breed Serangium japonicum is entered
Row research, more unstable indoor worm sources, cause Serangium japonicum large scale artificial breeding and discharge application to realize, because
This, is badly in need of setting up a kind of method of interior large-scale breeding Serangium japonicum.
However, during Serangium japonicum indoor propagation, the collection of pupa is that restriction Serangium japonicum is manually advised greatly
One significant obstacle of mould breeding because the happiness of Serangium japonicum mature larva to be clustered in old leaf blade back, plastic tub alms bowl edge recessed
In the interior or insectary of groove, other hidden corners are pupated, and more disperse, not easy-to-search and collection.There is no at present is effectively carried out day
The correlation technique that this Serangium japonicum pupa is collected, therefore cannot also realize indoor large-scale breeding Serangium japonicum.
Content of the invention
According to the habit of pupating of Serangium japonicum, the present inventor makes circle of pupating using single face corrugated board,
By reasonable employment, can not only effectively collect ladybug pupa, realize the centralized collection of pupa, the concentration of adult is sprouted wings, mating and spawning,
And the concentration of larval phase is raised, and the neat each worm state ladybug of development can be obtained, and because single face corrugated board is readily available,
Circle of pupating is simple for production, reusable, and pupa collection operating process is very convenient, can grind for Serangium japonicum related biological
Study carefully and artificial large-scale breeding provides important technical support.
I.e. the first object of the present invention is to provide a kind of easy to operate, collection of Serangium japonicum pupa that is efficiently being suitable for
Method, comprises the following steps：
A. Ladybird egg is obtained：The host plant of infection Bemisia tabaci is moved into grandis adults rearging cage, collects egg-laying season ladybug
The Ladybird egg produced at the host's blade back side by female adult pest；
B. children phase ladybug is raised：The host plant that step a is produced Ladybird egg moves into ladybug receptacle and raises；
C. ladybug pupa is collected：When ladybug children phase (ovum phase+larval phase) grows to the 12nd day, put in host's plant basal part of stem
Put circle of pupating；When children phase (ovum phase+larval phase) grows to the 14th～15 day, circle of pupating takes out, and is placed in grandis adults raising
In cage.
Preferably, in the collection method of Serangium japonicum pupa of the present invention, in the ladybug female adult pest of step a
Host plant infected with Bemisia tabaci, sustainable acquisition development condition relatively uniform Ladybird egg are changed in egg-laying season daily.
Preferably, in the collection method of Serangium japonicum pupa of the present invention, the feeding of the children phase ladybug of step b
The foster time is 14～15d.
In the present invention, term " corrugated paper " is the corrugated paper of the waveform formed by sheet lining and by corrugated roller processing
The plate object being bonded.Term " corrugated board (corrugated fiberboard) " is referred to by one or more layers corrugated paper
If the combination board being bonded between dried layer Paper or cardboard.Single face corrugated board is referred to by one layer or two-layer cardboard or paper, with one
The corrugated board that layer corrugated paper is bonded.Parameter index about single face corrugated board is referred to《Corrugated paper board standard GB/T/
T6544-2008》.
Preferably, in the collection method of Serangium japonicum pupa of the present invention, the circle of pupating of step c is employing
Single face corrugated board is made.
Preferably, in the collection method of Serangium japonicum pupa of the present invention, the circle of pupating be using long by 45～
50cm, the single face corrugated board of the strip of wide 6～7cm are made；It is highly preferred that the circle of pupating be using long 45～50cm,
The single face corrugated board of the strip of wide 6～7cm, along host's plant basal part of stem cincture, and is fixed into the circle of pupating of cylinder；More
Preferably, the circle of pupating is made using single face corrugated board, around 3～4 circle of host plant, highly 6～7cm, inner ring
3～4cm of footpath, the cylinder of 6～8cm of outer ring footpath are pupated circle；Most preferably, the circle of pupating is placed in matrix in host's plant basin alms bowl
On Nutrition Soil, first one layer of preservative film of pad on Culture media in host's plant basin alms bowl before circle is pupated in placement.
Preferably, in the collection method of Serangium japonicum pupa of the present invention, the host of the infection Bemisia tabaci plants
Strain preparation method be：The potted plant host plant of 8～10 leaf phases is taken, arrangement removal bottom old leaf only retains 4～5 and opens up completely
After opening leaf, Bemisia tabaci receptacle is moved into, being subsequently introduced the strain of Bemisia tabaci worm sources and worm host plant waiting interval and putting carries out connecing worm
Raise, shake worm sources strain makes Bemisia tabaci adults diffusion fly on worm host plant waiting and (is shaken every day 1 time), when host plant
On blade, Bemisia tabaci connects worm amount and reaches 10～15 adult/cm2When, worm sources strain is removed, standby after continuing to raise 1 week.Preferably,
In the present invention, the selected insect for raising ladybug is Bemisia tabaci, and the host plant of Bemisia tabaci is eggplant.
Therefore, another object of the present invention is to Serangium japonicum pupa is obtained by said method, and pass through the party
The Serangium japonicum pupa that method is obtained, the purposes in the artificial large-scale breeding of Serangium japonicum.
I.e. the invention provides a kind of artificial large-scale breeding method of Serangium japonicum, methods described is for employing as above
State the Serangium japonicum pupa of method collection.
Preferably, the artificial large-scale breeding method of Serangium japonicum of the present invention, including obtained said method
Serangium japonicum pupa, concentrates and is placed in grandis adults rearging cage, after 3～4 days when finding to have grandis adults to sprout wings, is raising
The host plant of infection Bemisia tabaci is placed in cage, and the new adult for sprouting wings can take food on host's Plant Leaf, mate, through this nursing 7
After～10 days, most of adult has mated and has spent preoviposition period.
Preferably, in the artificial large-scale breeding method of Serangium japonicum of the present invention, the grandis adults rearging cage
For being placed in insectary, the environment temperature of the insectary is 25～27 DEG C, relative humidity is RH 60%～65%, photoperiod
For L14h/D10h.
Preferably, the selected insect for raising ladybug in the present invention is Bemisia tabaci, and the host plant of Bemisia tabaci is
Eggplant.
From the foregoing, it will be observed that the large-scale breeding side of the collection method of the Serangium japonicum pupa of the present invention and Serangium japonicum
Method, at least has the advantage that：
The present invention makes full use of the habit of pupating of Serangium japonicum, using the convenient single face corrugated board system of material source
Pupate circle, preparation method is easy, it is easy that pupa collects operating process, can not only effectively collect ladybug pupa, it is achieved that the concentration of pupa
Collection, the advantage for concentrating raising for concentrating emergence, mating and spawning and larval phase of adult, and reusable, overcome
The happiness of usual Serangium japonicum mature larva be clustered in old leaf blade back, plastic tub alms bowl edge groove or in insectary other
Hidden corner is pupated, and more disperses, and easy-to-search and the difficult point that collects, can not meet the demand of natural enemy ladybug large-scale breeding,
Obtain the neat each worm state ladybug of development.
Bemisia tabaci host range is wide, field occurs extensively, as long as and reproductive capacity is strong, the generation cycle is short, indoor cyclically
Substitute host plant, higher population quantity just can be kept, prey of the present invention using Bemisia tabaci as breeding Serangium japonicum,
Method for breeding is easy, time saving and energy saving, it is easy to operate.Eggplant is one of the selection of Bemisia tabaci preference and optimal host plant, has
Have the characteristics that cultivation period is short, strong stress resistance, leaf area are big, host plant of the present invention using eggplant as breeding Bemisia tabaci, energy
Enough meet the needs of efficient, the quick breeding of prey Bemisia tabaci.Therefore using eggplant as the host plant of breeding Bemisia tabaci, with cigarette
Aleyrodid is raised in the present inventive method as the prey for raising Serangium japonicum, Serangium japonicum, per plant under normal circumstances
60～80 grandis adults can be obtained on eggplant plant, and each pair Serangium japonicum adult can breed 100～150 offsprings of acquisition
Adult, for prior art, it is achieved that Serangium japonicum efficiently, quickly, is in high volume bred.Therefore the present invention has section
Learn reasonable, simple, convenient and practical advantage.
Description of the drawings
Fig. 1 is the schematic diagram of the strip single face corrugated board of the embodiment of the present invention 1；
Fig. 2 is the photo of the strip single face corrugated board of the embodiment of the present invention 1；
Fig. 3 is the photo of the circle of pupating that the single face corrugated board of the embodiment of the present invention 1 is made.
Specific embodiment
A kind of easy to operate, the collection side of Serangium japonicum pupa that be efficiently suitable for is provided in an embodiment of the present invention
Method, comprises the following steps：
A. Ladybird egg is obtained：The host plant of infection Bemisia tabaci is moved into grandis adults rearging cage, collects egg-laying season ladybug
The Ladybird egg produced at the host's blade back side by female adult pest；
B. children phase ladybug is raised：The host plant that step a is produced Ladybird egg moves into ladybug receptacle and raises；
C. ladybug pupa is collected：When ladybug children phase (ovum phase+larval phase) grows to the 12nd day, put in host's plant basal part of stem
Put circle of pupating；When children phase (ovum phase+larval phase) grows to the 14th～15 day, circle of pupating takes out, and is placed in grandis adults raising
In cage.
In another embodiment of the present invention, change daily in the ladybug female adult pest egg-laying season of step a infected with
The host plant of Bemisia tabaci, the relatively uniform Ladybird egg of sustainable acquisition development condition.
In another embodiment of the present invention, the feeding time of the children phase ladybug of step b is 14～15d.
In another embodiment of the present invention, the circle of pupating of step c is by being made using single face corrugated board.As schemed
Shown in 1, the single face corrugated board is the corrugated board being bonded by two-layer cardboard and one layer of corrugated paper.
In another embodiment of the present invention, as shown in Fig. 2 the circle of pupating be using long 45～50cm, wide by 6～
The single face corrugated board of the strip of 7cm is made；In yet another embodiment of the present invention, the circle of pupating be using long by 45～
50cm, the single face corrugated board of the strip of wide 6～7cm, along host's plant basal part of stem cincture, and are fixed into pupating for cylinder
Circle；In yet another embodiment of the present invention, the circle of pupating is made using single face corrugated board, around host plant 3
～4 circles, highly 6～7cm, 3～4cm of inner ring footpath, the cylinder of 6～8cm of outer ring footpath are pupated circle；Another in the present invention is implemented
In example, the circle of pupating is placed in host's plant basin alms bowl on Culture media, first in host's plant basin before circle is pupated in placement
One layer of preservative film of pad on Culture media in alms bowl.
In another embodiment of the present invention, the preparation method of the host plant of the infection Bemisia tabaci is：Take 8～10
The potted plant host plant of piece leaf phase, after arrangement removal bottom old leaf only retains 4～5 fully expanded leaves, moves into Bemisia tabaci and raises
Room, being subsequently introduced the strain of Bemisia tabaci worm sources and worm host plant waiting interval and putting carries out connecing worm raising, and shake worm sources strain makes tobacco powder
The diffusion of lice adult flies on worm host plant waiting and (is shaken every day 1 time), when on host's plant leaf, Bemisia tabaci connects worm amount and reaches
To 10～15 adult/cm2When, worm sources strain is removed, standby after continuing to raise 1 week；Preferably, the host plant is eggplant.
Preferably, the selected insect for raising ladybug in the embodiment of the present invention is Bemisia tabaci, the host of Bemisia tabaci
Plant is eggplant.
In one embodiment of the invention, there is provided a kind of artificial large-scale breeding method of Serangium japonicum, described
Method is to employ the Serangium japonicum pupa that collects as above-mentioned method.
In another embodiment of the present invention, the artificial large-scale breeding method of Serangium japonicum of the present invention, bag
The Serangium japonicum pupa that said method is obtained is included, is concentrated and is placed in grandis adults rearging cage, when discovery has wooden dipper after 3～4 days
In rearging cage, the host plant of infection Bemisia tabaci during worm adult eclosion, is placed, the new adult for sprouting wings can be on host's Plant Leaf
Take food, mate, after this nursing 7～10 days, most of adult has mated and spent preoviposition period.
In another embodiment of the present invention, in the artificial large-scale breeding method of Serangium japonicum of the present invention,
For being placed in insectary, the environment temperature of the insectary is 25～27 DEG C to the grandis adults rearging cage, relative humidity is RH
60%～65%, the photoperiod is L14h/D10h.
Preferably, the selected insect for raising ladybug in the embodiment of the present invention is Bemisia tabaci, the host of Bemisia tabaci
Plant is eggplant.
Further technical scheme is illustrated below by way of specific embodiment, it should be understood that be only this below
Bright exemplary illustration, is not limited to the protection domain of the claims in the present invention.
Embodiment 1：The collection method of Serangium japonicum pupa
1st, step a Ladybird egg is obtained：The eggplant plant for having infected Bemisia tabaci is moved into grandis adults rearging cage and (adopts nylon
The conventional dependent insect cage that gauze makes, wide 40cm × depth 45cm × high 50cm), egg-laying season ladybug female adult pest can be automatically in eggplant leaf
The back side is laid eggs；Eggplant plant infected with Bemisia tabaci is changed daily, you can persistently obtain the relatively uniform Ladybird egg of development condition.
2nd, step b ladybug children phase is raised：The potted plant eggplant plant that step a is produced Ladybird egg moves into ladybug receptacle and raises
14～15d；
3rd, ladybug pupa is collected：When ladybug children phase (ovum phase+larval phase) grows to the 12nd day, put in eggplant plant basal part of stem
Put circle of pupating；When children phase (ovum phase+larval phase) grows to the 14th～15 day, circle of pupating takes out, and is placed in grandis adults raising
In cage.
Circle of wherein pupating makes and its uses technology：
Made using single face corrugated board, single face corrugated board is cut into the strip of long 45～50cm, wide 6～7cm first
Then above-mentioned strip corrugated board is enclosed around 3～4 along potted plant eggplant plant basal part of stem, and uses pin by shape (referring to Fig. 2)
It is fixed into 6～7cm of height, 3～4cm of inner ring footpath, the cylinder of 6～8cm of outer ring footpath to pupate circle, is placed in Culture media in basin alms bowl
On, water suction is enclosed in order to prevent from pupating make moist, first one layer of preservative film of pad on Culture media in basin alms bowl before circle is pupated in placement.
The acquisition of described infection Bemisia tabaci eggplant plant：The potted plant eggplant plant of 8～10 leaf phases is taken, is arranged and removes bottom
After portion's old leaf only retains 4～5 fully expanded leaves, Bemisia tabaci receptacle is moved into, is subsequently introduced the strain of Bemisia tabaci worm sources and worm waiting
Eggplant strain interval is put to carry out connecing worm raising, and shake worm sources strain makes Bemisia tabaci adults diffusion fly on worm eggplant plant waiting
(being shaken every day 1 time), when on eggplant plant leaf, Bemisia tabaci connects worm amount and reaches 10～15 adult/cm2When, worm sources strain is removed,
Standby after continuing to raise 1 week.
Embodiment 2：Pupate collecting effect evaluation of the circle to Serangium japonicum pupa
The happiness of Serangium japonicum mature larva be clustered in old leaf blade back, plastic tub alms bowl edge groove or insectary in its
He is hidden, and corner is pupated, and more disperses, it is difficult to centralized collection, therefore, how to improve the receipts of ladybug pupa in extensive raising
Collection efficiency, significant to improving reproductive efficiency.
1st, make ladybug with single face corrugated board as material to pupate circle.
First, strip corrugated board (as Fig. 2), length 45cm are cut into single face corrugated board, width separately design for
3.0cm, 4.0cm, 5.0cm, 6.0cm, 7.0cm, then, when ladybug children phase (ovum+larval phase) grows to the 12nd day, will be above-mentioned
Strip corrugated board along potted plant eggplant plant basal part of stem around 3 enclose, and with pin be fixed into cylinder pupate circle (as figure
3), corresponding circle height of pupating is respectively 3.0cm, 4.0cm, 5.0cm, 6.0cm, 7.0cm (A, B, C, D, E of corresponding Fig. 3).For
The circle water suction that prevents from pupating is made moist, one layer of the Culture media upper berth preservative film before circle of pupating is placed first in plastic tub alms bowl.
When children phase (ovum+larval phase) grows to the 14th～15 day, circle of pupating takes out, and counts ladybug on various strip corrugated boards
The quantity of pupa.Can pupate at the eggplant leaf back side in view of small part ladybug mature larva, this part ladybug pupa be easy to find and
Collect, therefore, pupa collection rate is calculated as follows：
4 instar larvae quantity × 100 before pupa collection rate (%)=(pupate circle pupa quantity+eggplant leaf pupa quantity)/pupate
2nd, result of the test
1 result of table shows, pupate the collecting effect significant difference of the circle to ladybug pupa, wherein, height 6.0cm of differing heights
Circle pupate to the collection rate of ladybug pupa respectively up to 92.14% and 94.98% with 7.0cm, be all remarkably higher than 3.0cm, 4.0cm,
5.0cm is highly pupated circle.Therefore, circle of pupating is made using the strip single face corrugated board of long 45cm, wide 6.0～7.0cm, can
The efficient collection of ladybug pupa is realized, easy to operate.
Table 1 is pupated collecting effect evaluation of the circle to Serangium japonicum pupa
Embodiment 3：The artificial large-scale breeding method of Serangium japonicum
The large scale artificial breeding of Serangium japonicum is the host plant using eggplant as breeding Bemisia tabaci, is made with Bemisia tabaci
For the prey of Serangium japonicum is raised, it is related to eggplant plant cultivating, the raising of prey Bemisia tabaci, the collection of Ladybird egg, ladybug children
Several processes such as the raising of worm phase, the collection of ladybug pupa and grandis adults raising.
1st, eggplant plant cultivating
(1) Culture media for nursery is bought in market, then Nutrition Soil is loaded the long 58cm × wide 30cm for getting ready
Seedlings nursing plate in standby；
(2) using eggplant as the host plant of breeding Bemisia tabaci, the eggplant seed of easy infection Bemisia tabaci is bought in market, is broadcast
Soaked 30 minutes with the carbendazim solution of active ingredient 0.1% before kind, then, seed is placed in wet gauze and is wrapped, be placed on
Vernalization under conditions of 28～30 DEG C, when the radicle for having 50% exposes by sow；
(3) type of seeding is 3～4g/m of sowing quantity by eggplant seed uniform broadcasting in seedlings nursing plate2, after planting with sieving
Nutrition Soil 1～2cm of earthing, is watered in time, is sufficiently humidified so as to Nutrition Soil；
(4) seedling culture frame is built in seedling raising greenhouse, frame divides 2 layers, leave the passage of convenient management between frame, nursery after planting
Disk is placed on seedling culture frame；
(5) water and fertilizer management, is watered when observing that Nutrition Soil dries within 2～3 days in time；
(6) carry out opportune thinning with pincet after eggplant is emerged, when observing that eggplant seedling growth and development state is not good enough with
Composite fertilizer or the low ratio of urea are watered to pour and apply；
(7) transplanted when eggplant seedling grows into 3～4 leaves, during transplanting, in diameter 18cm plastic tub alms bowl, loaded base
Matter Nutrition Soil, after pouring water, is implanted into plastic tub alms bowl with little medicine spoon from seedlings nursing plate lifting, transplants 1 plant per basin alms bowl, and after slow seedling, appropriateness adds
Strong water and fertilizer management；
(8) other insects such as red spider, thrips, aphid to be removed in seedling raising process in time, it is to avoid bring into Bemisia tabaci and
Ladybug receptacle.
2nd, the raising of prey Bemisia tabaci
(1) when eggplant plant strain growth is to 8～10 leaves, removes bottom old leaf and retain 4～5 fully expanded leaves, move into
Bemisia tabaci receptacle, is subsequently introduced the larger worm sources strain of density of Bemisia tabaci adults；
(2) worm sources strain and worm eggplant plant waiting are carried out connecing worm raising apart from one another by putting, shake worm sources strain makes tobacco powder
The diffusion of lice adult is flown on worm eggplant plant waiting；When on eggplant plant leaf, Bemisia tabaci connects worm amount and reach 10～15
Worm/cm2When, remove worm sources strain.
(3) raise interior and foster worm frame has been built, each foster worm frame divides three layers, per 55～60cm of floor height, depth 60cm, between frame
Leave the passage of convenient management；
(4) worm eggplant strain basin alms bowl has been connect and centralized management on foster worm frame has been moved to, before moving into Serangium japonicum receptacle
First raise 1 week in Bemisia tabaci receptacle；
(5) the eggplant plant of infection Bemisia tabaci is except for raising Serangium japonicum, also needing to stay a part as new
Worm sources strain, breeds for Bemisia tabaci, to maintain Bemisia tabaci quantity constant or increase；
(6) insectary control condition be 25～27 DEG C of temperature, humidity 60%～65%, photoperiod be L14/D10.
3rd, the amount reproduction of Serangium japonicum
(1) Ladybird egg is obtained：The eggplant strain for having infected Bemisia tabaci is moved into grandis adults rearging cage (using nylon gauze system
The conventional dependent insect cage of work, wide 40cm × depth 45cm × high 50cm), egg-laying season ladybug female adult pest can be produced at the eggplant leaf back side automatically
Ovum；Eggplant plant infected with Bemisia tabaci is changed daily, you can persistently obtain relatively uniform Ladybird egg；
(2) ladybug children phase is raised：The potted plant eggplant strain immigration ladybug receptacle for having Ladybird egg will be produced, now maintain plant life
Long good, so that Bemisia tabaci keeps enough population quantities, it is that ladybug provides sufficient prey.Raise interior and build foster worm
Frame, each foster worm frame divide three layers, per 55～60cm of floor height, depth 60cm, leaves the passage of convenient management, Japanese nose angle wooden dipper between frame
Worm is raised on a large scale and all carries out on foster worm frame.
(3) ladybug pupa is collected：When ladybug children phase (ovum+larval phase) grows to the 12nd day, place in eggplant strain basal part of stem
Pupate circle；When children phase (ovum+larval phase) grows to the 14th～15 day, circle of pupating takes out, while being planted with the careful picking of writing brush
The small part pupa of strain middle and lower part vacuum side of blade.
(4) grandis adults are raised：The same batch ladybug pupa that collects is concentrated and is placed in grandis adults rearging cage, after 3～4 days
When finding that grandis adults start to sprout wings, the eggplant plant for having infected Bemisia tabaci, the new adult meeting that sprouts wings is placed in rearging cage
Automatically take food on eggplant leaf, mate, after this raising 7～10 days, most of adult has mated and spent preoviposition period.
(5) insectary control condition is 25～27 DEG C of temperature, humidity 60%～65%, photoperiod L14/D10.
Eggplant plant is 8～10 leaf periods, retains 4～5 fully deployed blades after removing bottom old leaf.Eggplant plant
Upper Bemisia tabaci connects worm amount for 10～15 adult/cm2, after raising 1 week, introduce Serangium japonicum Adult worms producting eggs 1d.Through one
The raising of generation cycle, can raise 60～80 grandis adults of acquisition, each pair Serangium japonicum adult on every plant of eggplant plant
100～150 offspring adults of acquisition can be bred.
Circle of pupating is made using common single face corrugated board, first single face corrugated board is cut into long 45～50cm, wide by 6
The strip of～7cm, then encloses around 3～4 along eggplant strain basal part of stem, and is fixed into 6～7cm of height, inner ring footpath 3 with pin
～4cm, the cylinder of 6～8cm of outer ring footpath are pupated circle, are placed in basin alms bowl on Culture media, and the circle water suction in order to prevent from pupating is received
Tide, first one layer of preservative film of pad on Culture media in basin alms bowl before circle is pupated in placement.Serangium japonicum mature larva
Can down creep along eggplant strain cane during pupa, and pierce automatically pupate circle in, therefore, pupate circle for Serangium japonicum provide
One is concentrated the place that pupates, it is achieved that the efficient collection of ladybug pupa；Circle of pupating with pupa is just placed in imago breeding room
The adult of emergence can be obtained, eggplant plant of the basin infected with Bemisia tabaci in receptacle, is placed, new emergence grandis adults can be certainly
Move and take food, mate and lay eggs at the eggplant leaf back side；Eggplant plant infected with Bemisia tabaci is changed in the ladybug egg-laying season daily,
The relatively uniform Ladybird egg of i.e. sustainable acquisition.
Embodiment 4：Selectivity and suitable natural disposition of the Bemisia tabaci to host plant
1st, test method
Choice tests：Respectively pachyrhizus, cotton, eggplant, 4 kinds of cucumber is beaten for the fresh blade of examination host plant with card punch
Become the sequin of diameter 35mm, face of blade is layered on the training with cowling of diameter 110mm down along culture dish inner wall edge
(agar of the prior 12g/L of falling people in culture dish), random alignment in foster ware.Each culture dish accesses 100～150 pairs of Bemisia tabaci and becomes
It is inverted after worm, indoor light keeps uniform.After meeting worm 24h, the Bemisia tabaci adults in each host plant blades of investigation records fall
Borer population and ovum amount, are repeated 10 times.
Suitable natural disposition test：20 mixing age in days Bemisia tabaci female adult pest are taken, is fixed on blade cage each for examination host plant plant
Strain vacuum side of blade, after spawning 24h, removes blade cage and rejects the Bemisia tabaci adults on blade.Move to 27 DEG C of temperature, relatively wet
In the degree 65%, incubator of photoperiod L14/D10, observation day by day counts ovum and nymph survival rate, development duration and adult eclosion
Rate, often processes and is repeated 4 times.
2nd, result of the test
Host selection result of the test (table 2) shows that, after meeting worm 24h, Bemisia tabaci adults are on eggplant, the little roundleaf of cucumber
The worm amount that falls (respectively 85.50 and 84.10) is significantly higher than the worm amount (respectively 30.20 that falls on sweet potato and the little roundleaf of cotton
Head and 46.0)；Egg laying amount (respectively 882.80 and 681.50) of the Bemisia tabaci adults on eggplant and the little roundleaf of cucumber
Egg laying amount (respectively 143.30 and 239.30) also significantly greater than on sweet potato and the little roundleaf of cotton, shows Bemisia tabaci to not
Have with host plant and significantly take food and oviposition selectivity, adult substantially has a liking for eggplant and cucumber.
Host fits natural disposition result of the test (table 2) display, under 27 DEG C of indoor conditions, the generation development duration of Bemisia tabaci and deposits
Motility rate significant difference between different host plants, wherein, the generation development duration on cotton is most long (reaching 19.86d), significantly
It is longer than the generation development duration (respectively 18.08d, 17.53d and 17.92d) on eggplant, cucumber and sweet potato；On cotton
Generation survival rate minimum (only 56.89%), the generation survival rate on eggplant, cucumber may be up to 81.75% and 87.28%,
The generation survival rate being significantly higher than on cotton and sweet potato.
Summary result of the test shows, compared with sweet potato and cotton, it is obvious that eggplant and cucumber are not only Bemisia tabaci adults
Preference selects the host plant of (take food and lay eggs), and becomes apparent from suitable Bemisia tabaci offspring and grow, it is contemplated that cucumber
Belong to annual climber herbaceous plant, be not suitable for indoor culture, and eggplant belongs to semishrub plant, it is easy to indoor culture, therefore recognize
For, using eggplant as breeding Bemisia tabaci host plant more suitable.
Selectivity and suitable natural disposition of 2 Bemisia tabaci of table to different host plants
Embodiment 5：Selectivity and suitable natural disposition of the Serangium japonicum to host plant
1st, test method
Choice tests method：Eggplant, pachyrhizus, cotton, cucumber leaves are selected as test material, take certain amount mixing
Age in days Bemisia tabaci female adult pest, is individually fixed in the blade cage of diameter 30mm above-mentioned for examination host plant nothing the worm plant leaf back of the body
Face, after spawning 48h (ovum amount reaches more than 800), removes blade cage and rejects the Bemisia tabaci adults on blade, distinguished with card punch
Will be standby for each sequin for supplying the fresh blade of examination host plant to break into diameter 33cm with tobacco powder nit.(1) will band tobacco powder nit
4 kinds of host plant roundlet face of blades be layered in diameter 110cm culture dish along culture dish inner wall edge down, each culture
In ware, every kind of host plant respectively puts 1, random alignment, puts into egg-laying season Serangium japonicum female adult pest 1 per ware, then will training
Foster ware is inverted in photoperiod L14/D10,27 DEG C of temperature, the incubator of relative humidity 65%, checks each confession after 24h under anatomical lens
Prey tobacco powder nit amount and ladybug egg laying amount on examination host plant sequin, each process are repeated 10 times.(2) only cotton roundlet
On piece carry Bemisia tabaci ovum, other 3 kinds of host plant sequins all without tobacco powder nit, the same method of other steps (1), 24h
Each ladybug egg laying amount for examination host plant sequin on is checked afterwards, and each process is at least repeated 10 times.
Suitable natural disposition test method：(1) discharge observation is preyed on：Respectively by each confession with tobacco powder nit (ovum amount reaches more than 800)
Examination host plant sequin, face down are layered in the culture dish of diameter 35mm, put into egg-laying season ladybug female adult pest 1 per ware,
Then culture dish is inverted in the incubator of photoperiod L14/D10,27 DEG C of temperature, relative humidity 65%, after 24h under anatomical lens
Prey tobacco powder nit amount, often processes and is at least repeated 10 times.(2) reproductive capacity observation：Tobacco powder nit will be carried respectively, and (ovum amount reaches 800
More than grain) each for examination host plant sequin, face down is layered in the culture dish of diameter 35mm, plumage at the beginning of putting into 1 pair per ware
Change grandis adults, then culture dish is inverted in the incubator of photoperiod L14/D10,27 DEG C of temperature, relative humidity 65%, 24h
Afterwards adult is moved on on the host plant sequin of the same race with same food, under anatomical lens, observed and recorded replaces blade
On ladybug egg laying amount, so shift 1 time per 24h, until adult is dead, often process at least 10 pairs of adults of observation.(3) growth is sent out
Educate and survival rate observation：Process, by each in egg laying amount observation experiment, the ovum produced by grandis adults, move to 27 DEG C of temperature, relative humidity
Cultivate under conditions of 65% and photoperiod L14/D10, after egg hatching, newly hatched larvae is moved to band sufficient amount Bemisia tabaci adults
Each in examination host plant blades, observing the growing of each instar larvae, survival condition day by day with ovum, count each age children
The development duration of worm and survival rate.Often process initial worm amount and be 100 ovum.
2nd, result of the test
3 result of table shows, under the suitable alternative condition of prey quantity, Serangium japonicum eggplant, cucumber, cotton,
The selection rate of looking for food of pachyrhizus blade is respectively 73.67%, 15.45%, 7.17 and 3.71%, the amount of looking for food respectively 401.39,
108.94th, 58.44 and 20.50；Oviposition selection rate is respectively 72.75%, 18.55%, 0 and 8.70%, and egg laying amount is respectively
9.22nd, 3.17,0 and 1.67, difference all reaches the level of signifiance.In selectivity experiment in only cotton leaf with aleyrodid ovum, Japan
Serangium japonicum female adult pest is preyed on the cotton leaf with prey, but by big for ovum fecund on the eggplant leaf without prey, is shown
Serangium japonicum significant oviposition selectivity concrete to eggplant leaf.
Oviposition selection of 3 Serangium japonicum of table to different host plants
4 result of table shows that, in non-selection experiment, Serangium japonicum is to Bemisia tabaci on eggplant, cucumber, cotton and sweet potato
The predatory number of ovum is respectively 588.50,507.47,488.42 and 502.50, and difference is not notable.Serangium japonicum eggplant,
Average respectively 479.80,454.60,383.20 and 433.10 per female egg laying amount on cucumber, cotton and sweet potato, difference
Not notable；Serangium japonicum is 16.0d by the average development duration of ovum-become on eggplant, with the development duration on cotton
Quite, the development duration substantially less than on cucumber and sweet potato；Serangium japonicum completes the generation of development from generation to generation on eggplant
Survival rate is 37.93% and the generation survival rate (respectively 35.56% and 31.48%) on cucumber, sweet potato is suitable, significantly
It is higher than the generation survival rate 20.58% on cotton.
Summary result of the test shows, compared with sweet potato, cotton, cucumber, eggplant is not only Serangium japonicum adult
The Bemisia tabaci host plant that substantially preference is looked for food with oviposition selection, and substantially suitable ladybug offspring grows, and therefore recognizes
For breeding Bemisia tabaci with eggplant more suitable in order to indoor feeding Serangium japonicum.
Adaptability of 4 host plant of table to Serangium japonicum
Embodiment 6：The worm amount that connects when raising Bemisia tabaci using eggplant is selected
During Serangium japonicum being raised using eggplant on a large scale as host plant breeding Bemisia tabaci, eggplant is planted
In strain, Bemisia tabaci quantity directly affects the reproductive effect of Serangium japonicum.Bemisia tabaci quantity is too small to cause Japan
Serangium japonicum prey lazy weight, Bemisia tabaci quantity are excessive, and host plant may be caused aggrieved excessively, and in Japanese knife
Angle ladybug is occurred as soon as blade and wilts before not yet completing development from generation to generation and comes off, and impact Serangium japonicum is normally bred.Therefore, in profit
Bemisia tabaci is raised with eggplant plant as host plant is used in large-scale breeding Serangium japonicum, inquires into cigarette on host plant
Aleyrodid connect worm amount, to improve reproductive efficiency significant.
Test method：Test is provided with 5/cm2, 6～10/cm2, 11～15/cm2 and 16～20/cm24
Different Bemisia tabaci connect the process of worm amount, and it is to take the potted plant host plant of 8～10 leaf phases to connect worm method, arrange and only remove bottom old leaf
After retaining 4～5 fully expanded leaves, Bemisia tabaci receptacle is moved into, the strain of Bemisia tabaci worm sources is subsequently introduced with worm brinjal plant waiting
Interval is put carries out connecing worm raising, and shake worm sources strain makes Bemisia tabaci adults diffusion fly on worm eggplant plant waiting (to shake daily
Dynamic 1 time), when on eggplant plant leaf Bemisia tabaci connect worm amount reach each process connect worm amount when, remove worm sources strain, continue to raise 1 week
Life food as raising Serangium japonicum is provided afterwards, and observed and recorded eggplant plant situation and ladybug grow feelings day by day
Condition.
Result of the test：5 result of table shows, it is larger on Serangium japonicum breeding impact that Bemisia tabaci connects worm amount, when eggplant is planted
In strain, Bemisia tabaci meets 5/cm of worm amount2When, eggplant plant all energy normal growths, but on plant, Bemisia tabaci quantity is relatively low,
Prey wretched insufficiency, it is difficult to meet Serangium japonicum and grow needs, kills each other serious；When Bemisia tabaci on eggplant plant
Worm amount is connect for 6～10/cm2When, eggplant plant all energy normal growths, but on plant part, Bemisia tabaci quantity is lower slightly, hunts
Thing is not enough, there is a certain degree of autotomy phenomenon；When on eggplant plant, Bemisia tabaci connects worm amount for 11～15/cm2When, eggplant is planted
Strain all normal growths, on plant, Bemisia tabaci quantity is higher, and prey is sufficient, and ladybug grows normally；When on eggplant plant
Bemisia tabaci connects worm amount for 16～20/cm2When, on plant part, Bemisia tabaci quantity is too high, excessively causes harm, and causes eggplant plant
Cannot normal growth, occur as soon as blade and wilt before Serangium japonicum not yet completes from generation to generation development and come off.Summary result,
Think to select 10～15/cm2Worm amount is connect to the artificial biological control of Serangium japonicum as Bemisia tabaci on eggplant plant
Effect is best.
5 Bemisia tabaci of table connects the impact that worm amount completes generation developmental state to brinjal plant growth, ladybug
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of collection method of Serangium japonicum pupa, comprises the following steps：
A. Ladybird egg is obtained：Will infection Bemisia tabaci host plant move into grandis adults rearging cage, collect egg-laying season ladybug female become
The Ladybird egg produced at the host's blade back side by worm；
B. children phase ladybug is raised：The host plant that step a is produced Ladybird egg moves into ladybug receptacle and raises；
C. ladybug pupa is collected：When ladybug children phase (ovum phase+larval phase) grows to the 12nd day, in host's plant basal part of stem placementization
Pupa circle；When children phase (ovum phase+larval phase) grows to the 14th～15 day, circle of pupating takes out, and is placed in grandis adults rearging cage.
2. the collection method of Serangium japonicum pupa according to claim 1, it is characterised in that：Wooden dipper in step a
In the worm female adult pest egg-laying season, the host plant infected with Bemisia tabaci is changed daily, sustainable acquisition development condition is relatively uniform
Ladybird egg.
3. the collection method of Serangium japonicum pupa according to claim 1, it is characterised in that：The children phase of step b
The feeding time of ladybug is 14～15d.
4. the collection method of Serangium japonicum pupa according to claim 1, it is characterised in that：The pupating of step c
Circle is by being made using single face corrugated board.
5. the collection method of Serangium japonicum pupa according to claim 1, it is characterised in that：The circle of pupating is use
Long 45～50cm, the single face corrugated board of the strip of wide 6～7cm are made；Preferably, the circle of pupating be using long by 45～
50cm, the single face corrugated board of the strip of wide 6～7cm, along host's plant basal part of stem cincture, and are fixed into pupating for cylinder
Circle；It is highly preferred that the circle of pupating is made using single face corrugated board, around 3～4 circle of host plant, highly 6～7cm,
3～4cm of inner ring footpath, the cylinder of 6～8cm of outer ring footpath are pupated circle；Most preferably, the circle of pupating is placed in host's plant basin alms bowl
On Culture media, first one layer of preservative film of pad on Culture media in host's plant basin alms bowl before circle is pupated in placement.
6. according to the collection method of the Serangium japonicum pupa described in claim 1, it is characterised in that：Infection the posting of Bemisia tabaci
The preparation method of main plant is：Take the potted plant host plant of 8～10 leaf phases, arrange remove bottom old leaf only retain 4～5 complete
Complete launch leaf after, move into Bemisia tabaci receptacle, being subsequently introduced the strain of Bemisia tabaci worm sources and being put with worm host plant waiting interval is carried out
Worm raising is connect, shake worm sources strain makes Bemisia tabaci adults diffusion fly on worm host plant waiting and (is shaken every day 1 time), works as host
On plant leaf, Bemisia tabaci connects worm amount and reaches 10～15 adult/cm2When, worm sources strain is removed, standby after continuing to raise 1 week；Excellent
Selection of land, the host plant are eggplant.
7. a kind of artificial large-scale breeding method of Serangium japonicum, it is characterised in that methods described will for employing right such as
The Serangium japonicum pupa for asking 1-6 any one methods described to collect.
8. method according to claim 7, it is characterised in that the artificial large-scale breeding of the Serangium japonicum also includes
The step of Serangium japonicum pupa that claim 1-6 any one methods described is collected raises grandis adults.
9. method according to claim 7, it is characterised in that the step of the raising grandis adults being will will according to right
The Serangium japonicum pupa for asking the method described in 1～6 any one to obtain, concentrates and is placed in grandis adults rearging cage, 3～4 days
Afterwards when finding to have grandis adults to sprout wings, the host plant of infection Bemisia tabaci is placed in rearging cage, the new adult for sprouting wings can be
Take food on host's Plant Leaf, mate, after this raising 7～10 days, most of adult has mated and spent preoviposition period.
10. purposes according to claim 9, it is characterised in that the grandis adults rearging cage for being placed in insectary, institute
The environment temperature for stating insectary is 25～27 DEG C, relative humidity is L14h/D10h for RH 60%～65%, photoperiod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610924333.2A CN106472431B (en) | 2016-10-24 | 2016-10-24 | A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610924333.2A CN106472431B (en) | 2016-10-24 | 2016-10-24 | A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106472431A true CN106472431A (en) | 2017-03-08 |
| CN106472431B CN106472431B (en) | 2019-05-10 |
Family
ID=58270445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610924333.2A Active CN106472431B (en) | 2016-10-24 | 2016-10-24 | A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106472431B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107810930A (en) * | 2017-12-12 | 2018-03-20 | 福建省农业科学院植物保护研究所 | A kind of method for breeding of Serangium japonicum |
| CN111264476A (en) * | 2020-03-12 | 2020-06-12 | 四川省农业科学院经济作物育种栽培研究所 | Predatory ladybug egg mass collecting and collecting method and device |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1323520A (en) * | 2001-06-16 | 2001-11-28 | 王春良 | Industrial breeding technology of coccinella septempunctata |
| CN1631127A (en) * | 2005-01-26 | 2005-06-29 | 北京市农林科学院 | Artificial progagation method for ladybird beetle and lacewing fly |
| CN102017929A (en) * | 2009-09-10 | 2011-04-20 | 中国科学院动物研究所 | Method for artificially feeding ladybirds or lacewings by using lepidoptera insect larvae |
| CN102498985A (en) * | 2011-11-03 | 2012-06-20 | 福建省农业科学院植物保护研究所 | Method for preventing and controlling green soy bean bemisia tabaci by utilizing combination of Japanese serangium japonicum and trap crop |
| CN102948401A (en) * | 2012-11-01 | 2013-03-06 | 湖北省烟草公司恩施州公司 | artificial mass propagation method of harmonia axyridis |
| CN104430183A (en) * | 2013-09-13 | 2015-03-25 | 广西壮族自治区林业科学研究院 | Indoor large-scale breeding and feeding method for greater wax moth |
| CN104686453A (en) * | 2015-03-25 | 2015-06-10 | 湖南省植物保护研究所 | Living body cold storage method of serangium japonicum |
| CN104957107A (en) * | 2015-07-10 | 2015-10-07 | 山东省农业科学院植物保护研究所 | Method for propylea japonica indoor raising |
-
2016
- 2016-10-24 CN CN201610924333.2A patent/CN106472431B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1323520A (en) * | 2001-06-16 | 2001-11-28 | 王春良 | Industrial breeding technology of coccinella septempunctata |
| CN1631127A (en) * | 2005-01-26 | 2005-06-29 | 北京市农林科学院 | Artificial progagation method for ladybird beetle and lacewing fly |
| CN102017929A (en) * | 2009-09-10 | 2011-04-20 | 中国科学院动物研究所 | Method for artificially feeding ladybirds or lacewings by using lepidoptera insect larvae |
| CN102498985A (en) * | 2011-11-03 | 2012-06-20 | 福建省农业科学院植物保护研究所 | Method for preventing and controlling green soy bean bemisia tabaci by utilizing combination of Japanese serangium japonicum and trap crop |
| CN102948401A (en) * | 2012-11-01 | 2013-03-06 | 湖北省烟草公司恩施州公司 | artificial mass propagation method of harmonia axyridis |
| CN104430183A (en) * | 2013-09-13 | 2015-03-25 | 广西壮族自治区林业科学研究院 | Indoor large-scale breeding and feeding method for greater wax moth |
| CN104686453A (en) * | 2015-03-25 | 2015-06-10 | 湖南省植物保护研究所 | Living body cold storage method of serangium japonicum |
| CN104957107A (en) * | 2015-07-10 | 2015-10-07 | 山东省农业科学院植物保护研究所 | Method for propylea japonica indoor raising |
Non-Patent Citations (2)
| Title |
|---|
| 曾斌: "异色瓢虫人工大量繁殖与田间释放技术研究", 《中国优秀硕士学位论文全文数据库（农业科技辑）》 * |
| 陕西省植保检疫站: "《害虫天敌资源调查资料汇编（天敌昆虫部分）》", 31 October 1979 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107810930A (en) * | 2017-12-12 | 2018-03-20 | 福建省农业科学院植物保护研究所 | A kind of method for breeding of Serangium japonicum |
| CN111264476A (en) * | 2020-03-12 | 2020-06-12 | 四川省农业科学院经济作物育种栽培研究所 | Predatory ladybug egg mass collecting and collecting method and device |
| CN111264476B (en) * | 2020-03-12 | 2024-06-07 | 四川省农业科学院经济作物育种栽培研究所 | Predatory ladybug egg block collecting method and device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106472431B (en) | 2019-05-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105360021B (en) | A kind of cray is nursed young plants in hothouses device and rice field breeding round-robin method | |
| CN108056070B (en) | Method for breeding trichogramma by using snowflake moth | |
| CN103155906A (en) | Aphidoletes aphidimyza mass reproduction technology and application technology | |
| CN111328770B (en) | Indoor large-scale breeding method for nakayata nakamura | |
| CN113180011B (en) | Large-scale breeding method for Anagrus nilaparvatae | |
| CN103478117A (en) | Diffusion breeding and releasing method for preventing myzus persicae through harmonia axyridis | |
| CN110235860B (en) | Large-scale feeding method of Gray aphids | |
| CN106489846A (en) | Diamondback moth artificial propagation production method and its application in biological control | |
| CN113100002B (en) | Soybean-clanis bilineata tsingtauica one-year three-season planting and breeding mode | |
| CN105165726B (en) | A method of expanding numerous aphidius gifuensis using cuttage plant three-dimensional | |
| CN106472431B (en) | A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum | |
| CN113519468A (en) | Method for feeding phyllotreta striolata in indoor generation | |
| CN104604809A (en) | Biological control method using encarsia formosa for controlling tobacco whitefly | |
| CN110278922A (en) | Allomyrina dichotoma artificial diet technique | |
| CN113080144B (en) | Method and device for luring spodoptera frugiperda imagoes to intensively lay eggs and receive eggs | |
| CN111066732B (en) | Method for breeding orius sauteri | |
| CN112471082B (en) | Broadband preferred aphid breeding method | |
| CN109197786B (en) | Method for breeding large amount of semi-closed curvicauda curvularia indoors | |
| CN110574733B (en) | Method for breeding male bees of young Aphidius avenae with Aphidius gossypii | |
| CN108094331A (en) | A kind of narrow edge applies the indoor feeding method of noctuid | |
| CN112889761A (en) | Device for simulating field environment to efficiently breed insects | |
| CN111034684A (en) | Semitrella semipenguii seed conservation and rejuvenation breeding method | |
| CN111642465A (en) | Breeding method of Altai hepialus larvae | |
| CN216753288U (en) | Moth breeding device | |
| CN101940193B (en) | Method for indoor propagation and outdoor release of cybocephalus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |