CN107810930A - A kind of method for breeding of Serangium japonicum - Google Patents
A kind of method for breeding of Serangium japonicum Download PDFInfo
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- CN107810930A CN107810930A CN201711317211.8A CN201711317211A CN107810930A CN 107810930 A CN107810930 A CN 107810930A CN 201711317211 A CN201711317211 A CN 201711317211A CN 107810930 A CN107810930 A CN 107810930A
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- serangium japonicum
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- 241000132498 Serangium japonicum Species 0.000 title claims abstract description 55
- 230000001488 breeding effect Effects 0.000 title claims abstract description 25
- 238000009395 breeding Methods 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 22
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 29
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 29
- 210000004681 ovum Anatomy 0.000 claims abstract description 29
- 241000122098 Ephestia kuehniella Species 0.000 claims abstract description 25
- 241000238631 Hexapoda Species 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 15
- 241000208125 Nicotiana Species 0.000 claims abstract description 14
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 14
- 230000012447 hatching Effects 0.000 claims description 12
- 230000013011 mating Effects 0.000 claims description 8
- 230000019617 pupation Effects 0.000 claims description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 230000001850 reproductive effect Effects 0.000 abstract description 2
- 241000255749 Coccinellidae Species 0.000 description 12
- 235000013601 eggs Nutrition 0.000 description 10
- 241000254127 Bemisia tabaci Species 0.000 description 9
- 230000001418 larval effect Effects 0.000 description 7
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 210000003128 head Anatomy 0.000 description 5
- 230000017448 oviposition Effects 0.000 description 5
- 230000032669 eclosion Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 241000382353 Pupa Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 241000254124 Aleyrodidae Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 241000199695 Harmonia <beetle> Species 0.000 description 1
- 241000517325 Pediculus Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 235000021405 artificial diet Nutrition 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000004894 snout Anatomy 0.000 description 1
- 230000000192 social effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biomedical Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to insect to raise field, and in particular to a kind of method for breeding of Serangium japonicum larva.The present invention uses replacement prey of the Mediterranean flour moth ovum as Serangium japonicum, is raised.The replacement prey disclosure satisfy that the nutritional need to grow Serangium japonicum larva each age.Equally there is higher reproductive capacity after the imago feeding nature prey tobacco powder nit obtained through substituting prey raising.Method for breeding provided by the invention is simple to operation, is suitable for raising Serangium japonicum larva on a large scale, and the propagation in scale and application for Serangium japonicum provide the foundation and raised.
Description
Technical field
The present invention relates to insect to raise field, and in particular to a kind of method for breeding of Serangium japonicum.
Background technology
Tobacco powder Pediculus Semiptera Aleyrodidae, is a kind of global important pests, and its host is extensive, can be by directly piercing
Inhale, secretion honeydew induces the damage to crops such as dark mildew and transmitted virus disease.Agricultural is damaged as caused by Bemisia tabaci every year in the whole world
Lose and be up to tens billion of dollars, preventing and treating is extremely urgent.Bemisia tabaci depends on chemical prevention for a long time, excessively using insecticide
Cause to which create the serious resistance to the action of a drug.In the case where country pushes forward the background of agricultural chemicals zero growth rate comprehensively, it is badly in need of development Bemisia tabaci biology
Prevention Technique.
Serangium japonicum is the advantage predator of Bemisia tabaci, and widely distributed in China, each instar larvae and adult are equal
Can feeding difference worm state Bemisia tabaci, especially eating Bemisia tabaci ovum, can effectively control the growth of Bemisia tabaci quantity, this
It is significant in biological control.Meanwhile Serangium japonicum has the advantages that predatory number is big, growth cycle is short, Life of Adult is long,
Great development and application values.Therefore, it is biological control cigarette in the large-scale artificial reproduction technique of currently development Serangium japonicum
The effective way of aleyrodid, there is important social effect and ecological benefits.
Artificial large-scale breeding is the important foundation of natural enemy ladybug application.Traditional method for breeding depends on nature and hunted
Thing.The feeding system of this plant-insect-natural enemy is very big to the demand in manpower, space, and feeding cost is higher, it is difficult to long-term dimension
Hold.Therefore the emphasis of research natural enemy ladybug raising is turned into using man-made feeds or replacement prey.It is few due to substituting prey source,
Or nutrition can not meet that ladybug grows needs, therefore on less using the report for substituting prey raising ladybug, at present
Only harmonia axyridia realizes the report of biological control using drone larvae for replacement prey.Man-made feeds are to be based on pork liver mostly
The semi-fluid body feedstuff being configured to, occur after this feed of ladybug feeding growing slowly, phenomena such as adult weight loss, together
When, this kind of liquid feed easily lumps mouldy, and character is sticky to be also unfavorable for ladybug feeding, causes larval mortality very high.
In summary, also it is not carried out breakthrough progress in terms of the artificial large-scale breeding of ladybug, and Japanese knife
The research of the artificial diet technique of angle ladybug is even more to lag.Therefore, raised in the large-scale artificial for currently carrying out Serangium japonicum
The research for the technology of supporting, looks for a kind of source wide, and the food that substitutes easy to use turns into the key of Serangium japonicum artificial feeding.
The content of the invention
It is an object of the invention to：There is provided a kind of replacement prey of Serangium japonicum larva, it is intended to solve existing interior
During raising Serangium japonicum, limited because of the shortage of Bemisia tabaci worm sources and by season, can not large scale artificial breeding Japan knife
The problem of angle ladybug.
The invention provides a kind of method for breeding of Serangium japonicum, and Japanese nose angle wooden dipper is used as using Mediterranean flour moth ovum
The replacement prey of worm, is raised.
Preferably, the addition frequency for substituting prey is 2~3 days 1 time, and each addition is 0.08~0.12g/
100.
Preferably, the temperature of the raising is 25~30 DEG C, and humidity is 60~80%, and the photoperiod is：15L/9D~17L/
7D。
Preferably, the pairing of Serangium japonicum adult, mating, spawning are treated the egg hatching of Serangium japonicum by the present invention
After larva, fed with Mediterranean flour moth ovum, until larvae pupation, turns into adult.
Preferably, mating in insect box, spawning, the top of the insect box are placed in after the Serangium japonicum adult pairing
Portion carries passage.
Preferably, the insect box is cylinder, a diameter of 10~12 centimetres of the cylinder, a height of 5~8 centimetres.
Preferably, continue to feed the adult after the emergence with Mediterranean flour moth ovum or tobacco powder nit.
Beneficial effect：
The present invention uses replacement prey of the Mediterranean flour moth ovum as Serangium japonicum, and the replacement prey disclosure satisfy that
The nutritional need that Serangium japonicum each age grows.
Equally there is higher reproductive capacity after the imago feeding nature prey tobacco powder nit obtained through substituting prey raising.
Method for breeding provided by the invention is simple to operation, is suitable for raising Serangium japonicum on a large scale, is Japanese knife
The propagation in scale of angle ladybug and application provide the foundation.
Embodiment
The invention provides a kind of method for breeding of Serangium japonicum, and Japanese nose angle wooden dipper is used as using Mediterranean flour moth ovum
The replacement prey of worm, is raised.Mediterranean flour moth ovum disclosure satisfy that the nutrition need to grow Serangium japonicum each age
Ask, compared with the Serangium japonicum ladybug of natural prey tobacco powder nit raising, larval phase slightly extends；Pupa time difference does not show
Write；Survival rate of larvae, eclosion rate and adult weight differences are not notable.
The present invention matches Serangium japonicum adult, is laid eggs for mating, larva is obtained after hatching.In the present invention,
It is preferred that the Serangium japonicum kind worm after pairing is placed in insect box.The present invention is not special to the form and dimension of insect box
Limitation, as long as insect box can provide the activity space of suitable living environment and abundance for Serangium japonicum, beneficial to Japanese knife
The insect box that angle ladybug breeding activity is carried out is within protection scope of the present invention.The routine in this area can be used to support worm
Box.It is preferred that the top of insect box carries passage.In the specific embodiment of the invention, preferably described insect box is cylinder, institute
The diameter for stating cylinder is preferably 10~12 centimetres, more preferably 11 centimetres, and height is preferably 5~8 centimetres, more preferably 6 centimetres.
After the spawning of Serangium japonicum kind worm, ovum is placed under 26~28 DEG C of dark conditions, is preferably disposed in 27 DEG C of shading strips
Under part, hatching is larva after 4 days.The present invention feeds larva with Mediterranean flour moth ovum.In the present invention, it is preferred to every 2~3 days feed
1 Mediterranean flour moth ovum, it is furthermore preferred that feeding frequency between 2 days 1 time and 3 days 1 time alternately.Feed Mediterranean every time
The addition of powder snout moth's larva ovum is preferably 0.08~0.12g/100 heads, more preferably 0.1g/100 heads.The temperature of raising is preferably 25~
30 DEG C, more preferably 28 DEG C；The humidity of raising is preferably 60~80%, and more preferably 70%；The photoperiod of raising preferably controls
For：15L/9D~17L/7D, more preferably 16L/8D.
Larva raising is pupated after 8~10 days.After 4~5 days, pupa turns into adult.The present invention can use Mediterranean flour moth ovum
Or tobacco powder nit continues to feed the adult after the emergence.The adult after the emergence is fed with Mediterranean flour moth ovum, can be met
Adult grows the demand to nutritional ingredient.In the egg-laying season of Serangium japonicum adult, preferably raised with nature prey tobacco powder nit
Adult is fed, to meet the needs of adult breeding.The adult obtained using Mediterranean flour moth ovum raising Serangium japonicum larva,
Its adult stage is raised using nature prey tobacco powder nit, its preoviposition period, egg laying amount and egg hatching rate and whole generation
Compared using the Serangium japonicum adult of nature prey raising without significant difference.
The present invention is described in further detail with reference to embodiment, illustrated embodiment is only intended to explain this hair
It is bright, it is not intended to limit the scope of the present invention.
Embodiment 1
The present invention raises Serangium japonicum larva by the use of Mediterranean flour moth ovum as prey is substituted.
Rearing conditions：The raising temperature of Serangium japonicum larva is 28 DEG C, humidity 70%, photoperiod 16L/8D.
Method for breeding：The Serangium japonicum kind worm of pairing is put into 10 centimetres of diameter, high 5 centimetres, top carries
In the insect box of passage, its mating, spawning are made, egg hatching is fed to be put into Mediterranean flour moth ovum after larva, every 2 days
Once, addition is 0.08g/100 heads for addition.Until larvae pupation, can carry out outdoor natural enemy preventing and treating after emerging adult.
Embodiment 2
The present invention raises Serangium japonicum larva by the use of Mediterranean flour moth ovum as prey is substituted.
Rearing conditions：The raising temperature of Serangium japonicum larva is 26 DEG C, humidity 65%, photoperiod 17L/7D.
Method for breeding：The Serangium japonicum kind worm of pairing is put into 11 centimetres of diameter, high 6 centimetres, top carries
In the insect box of passage, its mating, spawning are made, egg hatching is fed to be put into Mediterranean flour moth ovum after larva, every 3 days
Once, addition is 0.12g/100 heads for addition.Until larvae pupation, can carry out outdoor natural enemy preventing and treating after emerging adult.
Embodiment 3
The present invention raises Serangium japonicum larva by the use of Mediterranean flour moth ovum as prey is substituted.
Rearing conditions：The raising temperature of Serangium japonicum larva is 29 DEG C, humidity 75%, photoperiod 15L/9D.
Method for breeding：The Serangium japonicum kind worm of pairing is put into 12 centimetres of diameter, high 7 centimetres, top carries
In the insect box of passage, its mating, spawning are made, egg hatching is fed to be put into Mediterranean flour moth ovum after larva, every 2 days
Once, addition is 0.09g/100 heads for addition.Until larvae pupation, can carry out outdoor natural enemy preventing and treating after emerging adult.
The application effect of the present invention is described in detail with reference to experiment.
Embodiment 4：Substitute the identification of prey feeding effect
A. the acquisition of Serangium japonicum larva：Capture from the indoor Japanese nose angle wooden dipper using the raising of nature prey Bemisia tabaci
Worm adult, its male and female is matched, is placed in the foster worm ware of agar moisturizing, laying has the cotton leaf of tobacco powder nit above agar.
After laying eggs, 27 DEG C are placed on, is hatched under conditions of 70% relative humidity, 1 instar larvae after hatching is used for measuring.
B. the influence that prey grows to Serangium japonicum is substituted：10 larvas are a repetition, are repeated 3 times altogether.
Processing 1 uses tobacco powder nit raising (control group) using the replacement prey Mediterranean flour moth ovum raising in embodiment 1, processing 2, often
Day observes and records development duration, survival rate, eclosion rate, adult body weight, the Life of Adult of every larva, until adult is all dead
Die, the results are shown in Table 1.
C. Serangium japonicum larval phase, carries out raising to pupating using prey is substituted, and after adult eclosion, picking 10 is right
Adult is matched, and is then raised with nature prey, observes and records the spawning situation of female adult daily, and the ovum produced is taken out and seen
Examine its larvae hatch situation.Using larval phase with the Serangium japonicum that nature prey is raised as control, compare two groups of processing nose angles
The difference of the indexs such as the preoviposition period of ladybug, egg laying amount and egg hatching rate, the results are shown in Table 2.
The result of table 1 shows that Serangium japonicum larva after substituting the raising of prey Mediterranean flour moth ovum, go through by its larvae development
The larvae development of phase more natural prey tobacco powder nit raising goes through phase extension, but its pupa time, survival rate of larvae, eclosion rate and into polypide
Wait biological parameter compared with natural prey tobacco powder nit is raised without significant difference again.
The result of table 2 shows that larval phase raises the grandis adults of acquisition using prey Mediterranean flour moth ovum is substituted, in its adult
Stage is raised using nature prey tobacco powder nit, and its preoviposition period, egg laying amount and egg hatching rate are with whole from generation to generation using certainly
The grandis adults of right prey raising are compared without significant difference.
Summary result shows, substitutes prey Mediterranean flour moth ovum and disclosure satisfy that Serangium japonicum larval growth is developed
Demand, can be used for Serangium japonicum larval phase raising.Larval phase can be used as in the case of natural prey shortage indoors
Replacement food be used for maintain and expand population, solve an important step during large-scale breeding.
Table 1：Substitute the influence that prey grows to Serangium japonicum
Note：Institute's column data is mean+SD in table, and same column same letter represents that difference is not notable, same column same word
Matrix shows significant difference.Similarly hereinafter
Table 2：Substitute influence of the prey to Serangium japonicum offspring's fecundity
| Processing | Preoviposition period (my god) | Total egg laying amount (grain/female) | Egg hatching rate (%) |
| 1 | 5.5±0.2a | 329.8±15.3a | 82.7±3.1a |
| 2 | 5.6±0.3a | 351.5±37.4a | 83.1±4.5a |
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of method for breeding of Serangium japonicum, it is characterised in that be used as Serangium japonicum using Mediterranean flour moth ovum
Replacement prey, raised.
2. method for breeding according to claim 1, it is characterised in that the addition frequency for substituting prey is 2~3 days 1
Secondary, each addition is 0.08~0.12g/100 heads.
3. method for breeding according to claim 1, it is characterised in that the temperature of the raising is 25~30 DEG C, and humidity is
60~80%, the photoperiod is：15L/9D~17L/7D.
4. according to the method for breeding described in claims 1 to 3 any one, it is characterised in that Serangium japonicum adult is matched somebody with somebody
To, mating, spawning, after the egg hatching of Serangium japonicum is larva, fed with Mediterranean flour moth ovum, until larvae pupation,
Turn into adult.
5. method for breeding according to claim 4, it is characterised in that be placed in after the Serangium japonicum adult pairing foster
Mating, spawning in worm box, the top of the insect box carries passage.
6. method for breeding according to claim 5, it is characterised in that the insect box is cylinder, the cylinder
A diameter of 10~12 centimetres, a height of 5~8 centimetres.
7. method for breeding according to claim 4, it is characterised in that continue to feed with Mediterranean flour moth ovum or tobacco powder nit
Adult after the emergence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711317211.8A CN107810930A (en) | 2017-12-12 | 2017-12-12 | A kind of method for breeding of Serangium japonicum |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711317211.8A CN107810930A (en) | 2017-12-12 | 2017-12-12 | A kind of method for breeding of Serangium japonicum |
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| CN107810930A true CN107810930A (en) | 2018-03-20 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973784A (en) * | 2021-12-09 | 2022-01-28 | 河南农业大学 | Method for preventing insects, ant lions, from killing each other by artificial feeding and application thereof |
| CN115428770A (en) * | 2022-11-09 | 2022-12-06 | 云南省烟草公司昆明市公司 | Method for adjusting egg laying time of ladybug by using alternative feed |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532587A (en) * | 2016-03-13 | 2016-05-04 | 福建艳璇生物防治技术有限公司 | Anagasta kuehniella breeding method and application |
| CN106472431A (en) * | 2016-10-24 | 2017-03-08 | 福建省农业科学院植物保护研究所 | A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum |
-
2017
- 2017-12-12 CN CN201711317211.8A patent/CN107810930A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532587A (en) * | 2016-03-13 | 2016-05-04 | 福建艳璇生物防治技术有限公司 | Anagasta kuehniella breeding method and application |
| CN106472431A (en) * | 2016-10-24 | 2017-03-08 | 福建省农业科学院植物保护研究所 | A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973784A (en) * | 2021-12-09 | 2022-01-28 | 河南农业大学 | Method for preventing insects, ant lions, from killing each other by artificial feeding and application thereof |
| CN113973784B (en) * | 2021-12-09 | 2023-06-02 | 河南农业大学 | Method for preventing insect ants and lions from killing each other by artificial feeding and application thereof |
| CN115428770A (en) * | 2022-11-09 | 2022-12-06 | 云南省烟草公司昆明市公司 | Method for adjusting egg laying time of ladybug by using alternative feed |
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