CN105532587A - Anagasta kuehniella breeding method and application - Google Patents
Anagasta kuehniella breeding method and application Download PDFInfo
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- CN105532587A CN105532587A CN201610138908.8A CN201610138908A CN105532587A CN 105532587 A CN105532587 A CN 105532587A CN 201610138908 A CN201610138908 A CN 201610138908A CN 105532587 A CN105532587 A CN 105532587A
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- 241000122098 Ephestia kuehniella Species 0.000 title claims abstract description 55
- 238000009395 breeding Methods 0.000 title abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 30
- 241000255749 Coccinellidae Species 0.000 claims abstract description 15
- 241000238876 Acari Species 0.000 claims abstract description 11
- 235000013601 eggs Nutrition 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 8
- 241001414989 Thysanoptera Species 0.000 claims abstract description 5
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 5
- 102000002322 Egg Proteins Human genes 0.000 claims description 22
- 108010000912 Egg Proteins Proteins 0.000 claims description 22
- 210000004681 ovum Anatomy 0.000 claims description 22
- 238000010010 raising Methods 0.000 claims description 21
- 235000013339 cereals Nutrition 0.000 claims description 16
- 235000013312 flour Nutrition 0.000 claims description 11
- 239000002361 compost Substances 0.000 claims description 10
- 241001635552 Anthocoridae Species 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 5
- 241001292007 Chrysopa Species 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 241001124076 Aphididae Species 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 240000006394 Sorghum bicolor Species 0.000 claims description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 3
- 241000275633 Syrphus Species 0.000 claims description 3
- 241001454293 Tetranychus urticae Species 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 230000008014 freezing Effects 0.000 claims description 2
- 238000007710 freezing Methods 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 241000256618 Trichogramma Species 0.000 abstract description 3
- 241000258923 Neuroptera Species 0.000 abstract description 2
- 235000021190 leftovers Nutrition 0.000 abstract 2
- 241000255925 Diptera Species 0.000 abstract 1
- 241001647846 Orius insidiosus Species 0.000 abstract 1
- 241001238248 Trichogramma japonicum Species 0.000 abstract 1
- 241001481304 Vespoidea Species 0.000 abstract 1
- 241000406588 Amblyseius Species 0.000 description 18
- 230000017448 oviposition Effects 0.000 description 5
- 235000013305 food Nutrition 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 241001658758 Neoseiulus Species 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- 241001481659 Syrphidae Species 0.000 description 2
- 241000256680 Trichogrammatidae Species 0.000 description 2
- 241001621310 Typhlodromips Species 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 235000021393 food security Nutrition 0.000 description 2
- 241001541106 Amblydromalus Species 0.000 description 1
- 241001156090 Amblyseius orientalis Species 0.000 description 1
- 241001064674 Chrysopa septempunctata Species 0.000 description 1
- 241000258936 Chrysoperla plorabunda Species 0.000 description 1
- 241000258935 Chrysopidae Species 0.000 description 1
- 241001414720 Cicadellidae Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 241000199695 Harmonia <beetle> Species 0.000 description 1
- 241001455174 Iphiseius Species 0.000 description 1
- 241001206567 Neoseiulus barkeri Species 0.000 description 1
- 241001455175 Neoseiulus cucumeris Species 0.000 description 1
- 241001635529 Orius Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241001474977 Palla Species 0.000 description 1
- 241000132498 Serangium japonicum Species 0.000 description 1
- 241000186214 Trichogramma dendrolimi Species 0.000 description 1
- 241001248508 Trichogramma evanescens Species 0.000 description 1
- 241000406562 Trichogramma ostriniae Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000013348 organic food Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to an anagasta kuehniella breeding method and application. Grain and grain leftovers are independently used for mixed according to a certain ratio to be used. The grain or the grain leftovers or mixed feed serves as an artificial breeding material for breeding anagasta kuehniella. Bred female adult anagasta kuehniella is put in a container, eggs are put in a refrigerator with the temperature of 4-8 DEG C or irradiated by ultraviolet rays for standby application after being laid by anagasta kuehniella and collected, and the eggs can be directly used for breeding various types of ladybugs, predatory mites, lacewing flies, orius insidiosus, thrips and other natural enemies and producing the natural enemies on a large scale. The anagasta kuehniella eggs can also be used as hosts of various egg parasitic wasps such as trichogramma and trichogramma japonicum ashmead after being irradiated by ultraviolet rays. Larvae and eggs, produced through the method, of anagasta kuehniella can be used for producing various types of natural enemy insects and predatory and carnivorous mites, and therefore harm of pests and pest mites to agricultural and forestry crops is reduced.
Description
Technical field
The present invention relates to Mediterranean flour moth training raising method and application.
Technical background
Current guarantee food security has caused the very big attention of national governments and the common people, and in food security, the agricultural chemicals of crops frequently uses and causes food, ecological pollution problem is the problem that people pay close attention to always.How to reduce the problem that pesticide pollution has become various countries Agricultural Scientist, functional government departments are studied always of crops, therefore, how few spray or pesticide can not control again the research of insect pest, the problem of exploitation has been discharged in face of people.Each insect has its natural enemy to control, but natural enemy has its common feature: following phenomenon and insect, evil mite there occurs, natural enemy has had food population just to catch up with, in addition owing to frequently using agricultural chemicals, worsen natural enemy living environment, therefore need all kinds of natural enemy release of large scale artificial breeding to field, make up the problem of natural world natural enemy deficiency.But natural enemy domestication, raise very difficult, particularly the artificial feed of natural enemy are a most important thing difficult problems.The extensive artificial cultivation of such as Mediterranean flour moth (Ephestiakuehniella) is a technical barrier always.
Summary of the invention
Task of the present invention is to provide the extensive rearing method of Mediterranean flour moth (Ephestiakuehniella) and application.
Mediterranean flour moth raising method of the present invention is that following technical scheme realizes:
With grain as flour (fine powder, secondary powder), corn flour, sorghum flour etc., or grain leftover as material is as wheat bran, wheat skin, rice bran etc.Used aloned also with by a certain percentage used in combination of this two class (grain and grain leftover as material), wherein can add dusty yeast or the organic matter such as the matrix containing zymasis or protein, also can not add.These grains or offcuts or mixed feed can be used as the artificial composts or fertilisers of cultivating of joining foster Mediterranean flour moth.These composts or fertilisers of cultivating can meet that Mediterranean flour moth grows, grows, required nutrition of laying eggs.To carry out disinfection in order to avoid because insect, evil mite or living contaminants cause raising unsuccessfully in matrix before described various composts or fertilisers of cultivating use, sterilization method: a, composts or fertilisers of cultivating to be placed on subzero refrigerator freezing a couple of days for subsequent use, or b, composts or fertilisers of cultivating is put in 60 DEG C of-120 DEG C of baking ovens dry 2-10 hour for subsequent use.
The technological process of the raising method of described Mediterranean flour moth is as follows:
(1), by by a certain percentage used in combination of load weighted flour (fine powder or secondary powder), corn flour, sorghum flour, corn flour or grain and grain leftover as material, wherein can add dusty yeast or the matrix containing zymasis, also can not add, respectively they be put into one and breathe freely and the culture vessel that can prevent from runing away or carrier;
(2) in culture vessel, the kind ovum of the Mediterranean flour moth of varying number, is then added according to container size;
(3), will join foster container or carrier and introduce culturing room and cultivate, condition of culture: temperature is: 15-36 DEG C, relative air humidity is 50-100%RH, with or without illumination, adds the kind ovum of varying number Mediterranean flour moth according to container size.The generation cycle of Mediterranean flour moth is different according to composts or fertilisers of cultivating nutrient component, temperature, when cultivation temperature is 20-36 DEG C, and general cultivation 30 days-60 days.DIFFERENT FEED produces breeding time of Mediterranean flour moth and egg laying amount in table 1.
The female adult pest of the Mediterranean flour moth that the present invention turns out is placed in special container, treats that it is laid eggs, and places in the refrigerator of 4-8 degree stand-by after collecting ovum.These ovum can directly be used for feeding and the natural enemy such as the various ladybug of large-scale production, Predatory Mites, Chrysopa, minute pirate bugs, thrips.
Raising method of the present invention cultivates the ovum of acquisition Mediterranean flour moth in order to raise other natural enemy, can raise separately, also can add the raisings such as aphid, pollen, two spotted spider mite in feeding process with the ovum of Mediterranean flour moth.
With Mediterranean flour moth cultural method of the present invention, the ovum of the Mediterranean flour moth of collecting, can as the alternative feed of predacious mites or carnivorous mite class or prey.Concrete predacious mites or carnivorous mite class are as Si Shi genus amblyseius berlese (Amblyseius (Typhlodromips) swiriskiiAthias-Henriot), the new little peaceful mite in California (Neoseiulus (Amblyseius) californicus (McGregor)), pseudo-genus amblyseius berlese (Neoseiulus (Amblyseius) fallacics (Garman)), source, river genus amblyseius berlese (AmblyseiuseharaiAmitai & Swirskii), the new little peaceful mite (Neoseiulus (Amblyseius) of cucumber, the new little peaceful mite of Pasteur, the nearly blind peaceful mite of lemon (Amblydromalus (Amblyseius) limonicus (Garman & McGregor), Anderson genus amblyseius berlese (AmblyseiusandersoniCHant), regression genus amblyseius berlese (Amblyseius (Iphiseius) degeneransBerlese), a kind of little blind peaceful mite (Typhlodromips (Transeius) Schicha), the very peaceful mite (EuseiusgallicusKreiter & Tixier) of one, the narrow lower blunt mite of point, the narrow lower blunt mite of soldier, Amblyseius Orientalis Ehara.Part Predatory Mites is raised in table 2 with Mediterranean flour moth ovum.
The Mediterranean flour moth ovum cultivated by method of the present invention can as the host of the various egg parasitoids such as oophagous trichogrammae, Trichogramma after Ultraviolet radiation process.The ovum of the Mediterranean flour moth of collecting, can as the alternative feed of ladybug or prey.Concrete ladybug is as the harmonia axyridia (ladybug of the Coccinellidae (Coccinellidae) such as LeisAxyridis (Pallas), little black ladybug, Serangium japonicum, bright red ladybug; Oophagous trichogrammae (Trichogrammatidae.) is as the oophagous trichogrammae of the ovum parasitisms such as trichogramma evanescens, trichogramma dendrolimi, Trichogramma Ostriniae, poison moth oophagous trichogrammae, Trichogramma, boat moth oophagous trichogrammae, leafhopper oophagous trichogrammae; Chrysopa (.Chrysopidae) is as Chinese green lacewing, chrysopa septempunctata etc.; Syrphus fly (Syrphid) etc.; Minute pirate bugs (Flowerbug) is as minute pirate bugs, Orius, Similis, small minute pirate bugs etc.The prey aphid raising ladybug egg laying amount and ladybug with Mediterranean flour moth ovum raises foster ladybug egg laying amount quite, and the egg hatching rate of institute's output is not affected.Mediterranean flour moth plays very important effect for output and quality in the extensive industrialization of natural enemy is raised.Compared with the prior art, the present invention is invention Mediterranean flour moth (Ephestiakuehniella) cultural method successfully, and successfully utilizes the ovum of Mediterranean flour moth to raise the natural enemies such as ladybug (ladybird), Chrysopa (Lacewing), oophagous trichogrammae (Trichogrammatidae), Predatory Mites (Predatorymites), thrips (Thrips), syrphus fly (Syrphid).Minute pirate bugs is raised with Mediterranean flour moth 1,2 instar larvae.Utilize that this method produces the larva of Mediterranean flour moth, ovum can be used for producing various natural enemy insect and predatism, carnivorous mite class, thus reduce insect, evil mite to the harm of agriculture, woods crop.This invention will reduce Pesticide use for China's agricultural, produce nuisanceless, organic food, preserve the ecological environment and make important contribution.
table 1 Mediterranean flour moth takes food the biological property of different food
25℃
table 2 utilizes Mediterranean flour moth ovum for the biological property of the different Predatory Mites of diet
25℃
| Predatory Mites kind | The ovum phase | Children's mite phase | If the I mite phase | If II mite | Preoviposition period | From generation to generation | Egg-laying period | Total egg laying amount |
| Amblyseius cucumeris oudeman | 1.63±0.57 | 1.00±0.32 | 2.22±0.41 | 2.13±0.71 | 2.68±0.71 | 9.68±0.60 | 60.06±1.98 | 80.36±7.89 |
| Si Shi genus amblyseius berlese | 1.36±0.25 | 0.86±1.6 | 1.73±0.48 | 2.04±0.71 | 1.64±1.00 | 7.60±1.24 | 44.56±1.89 | 54.56±3.33 |
| Amblyseius barkeri | 1.83±0. 75 | 1.20±0.12 | 2.27±0.21 | 2.23±0.81 | 2.48±0.61 | 10.01±0.6 | 50.06±1.08 | 53.63±7.19 |
| California genus amblyseius berlese | 1.45±0.17 | 0.77±0.27 | 1.38±0.33 | 1.25±0.22 | 1.35±0.22 | 6.20±0.24 | 46.56±1.29 | 50.33±2.10 |
| Pseudo-blunt genus amblyseius berlese | 1.50±0.37 | 0.75±0.37 | 1.50±0.54 | 1.30±0.23 | 1.50±0.27 | 6.55±0.47 | 48.67±1.81 | 58.13±1.20 |
| The former genus amblyseius berlese in river | 1.70±0.51 | 0.90±0.17 | 1.05±0.20 | 1.20±0.51 | 1.85±0.22 | 6.70±0.36 | 40.26±1.39 | 52.57±2.23 |
| Become mildewed genus amblyseius berlese | 1.50±0.27 | 0.75±0.27 | 1.50±0.30 | 1.51±0.23 | 1.75±0.44 | 7.10±0.41 | 55.11±1.45 | 58.13±5.32 3 --> |
| Amblyseius persidolongispinosus | 1.55±0.51 | 0.85±0.37 | 1.55±0.44 | 1.45±0.33 | 1.90±0.11 | 7.30±0.35 | 48.25±1.43 | 50.43±1.11 |
| The nearly blind peaceful mite of lemon | 1.59±0.52 | 1.02±0.54 | 1.58±0.19 | 1.65±0.34 | 1.58±0.23 | 7.42±0.36 | 55.16±1.29 | 51.63±2.22 |
| Anderson genus amblyseius berlese | 2.2±0.53 | 0.77±0.51 | 1.55±0.19 | 1.33±0.54 | 1.97±0.56 | 7.82±0.47 | 53.28±1.23 | 55.66±3.12 |
Claims (6)
1. Mediterranean flour moth cultural method, it is characterized in that: described Mediterranean flour moth cultural method is with grain or grain leftover as material, used aloned, or with by a certain percentage used in combination of grain and grain leftover as material, these grains or offcuts or mixed feed are as the artificial composts or fertilisers of cultivating of joining foster Mediterranean flour moth, will carry out disinfection before described various artificial composts or fertilisers of cultivating uses, sterilization method: a, artificial composts or fertilisers of cultivating to be placed on subzero refrigerator freezing a couple of days for subsequent use; Or b, artificial composts or fertilisers of cultivating is put in 60 DEG C of-120 DEG C of baking ovens dry 2-10 hour for subsequent use;
The technological process of the raising method of described Mediterranean flour moth is as follows:
(1). by by a certain percentage used in combination of load weighted flour, corn flour, sorghum flour, corn flour or grain and grain leftover as material, respectively they are put into a ventilative and culture vessel that can prevent from runing away;
(2). in culture vessel, the kind ovum of the Mediterranean flour moth of varying number is then added according to container size;
(3). culture vessel or carrier are introduced culturing room and cultivates, condition of culture: temperature is: 15-36 DEG C, relative air humidity is 50-100%RH, as cultivation temperature 20-36 DEG C, general cultivation 30 days-60 days;
The female adult pest of the Mediterranean flour moth turned out is placed in a reservoir, treats that it is laid eggs, and places in the refrigerator of 4-8 degree stand-by after collecting ovum.
2. the raising method of Mediterranean flour moth according to claim 1, is characterized in that, described grain or grain leftover as material add dusty yeast or the organic substance such as the matrix containing zymasis or protein matrix.
3. the application of the raising method of Mediterranean flour moth according to claim 1 and 2, it is characterized in that, the raising method of described Mediterranean flour moth obtains the ovum of Mediterranean flour moth in order to raise natural enemy, can raise with the ovum of Mediterranean flour moth separately, also can add aphid, pollen, two spotted spider mite raising in feeding process.
4. the application of the raising method of the Mediterranean flour moth according to claim 1 or 2 or 3, is characterized in that, Mediterranean flour moth 1,2 instar larvae that described raising method is turned out is for raising minute pirate bugs.
5. the application of the raising method of the Mediterranean flour moth according to claim 1 or 2 or 3, is characterized in that, the ovum of the Mediterranean flour moth that described raising method is turned out, as alternative feed or the prey of predacious mites or carnivorous mite class.
6. the application of the raising method of the Mediterranean flour moth according to claim 1 or 2 or 3, is characterized in that, described raising method turns out Mediterranean flour moth ovum for raising ladybug, Chrysopa, oophagous trichogrammae, thrips, syrphus fly.
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| CN107810930A (en) * | 2017-12-12 | 2018-03-20 | 福建省农业科学院植物保护研究所 | A kind of method for breeding of Serangium japonicum |
| CN108496898A (en) * | 2018-03-18 | 2018-09-07 | 福建省农业科学院植物保护研究所 | A method of manually substituting the few hair amblyseius as predatory mite of feed factory metaplasia production |
| CN111028252A (en) * | 2019-05-12 | 2020-04-17 | 李明霞 | Object instant delivery platform |
| CN113749055A (en) * | 2021-08-26 | 2021-12-07 | 中山大学 | Method for breeding rice ladybug by using Mediterranean meal moth eggs and rape pollen mixed food |
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| 杜艳丽等: "烟草粉螟Ephestia elutella 的生物学特性", 《山地农业生物学报》 * |
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| CN106359314B (en) * | 2016-10-21 | 2019-05-24 | 福建省农业科学院植物保护研究所 | With the method and purposes of a kind of formula mass propagation harmonia axyridia of Alternative Food |
| CN107810930A (en) * | 2017-12-12 | 2018-03-20 | 福建省农业科学院植物保护研究所 | A kind of method for breeding of Serangium japonicum |
| CN108496898A (en) * | 2018-03-18 | 2018-09-07 | 福建省农业科学院植物保护研究所 | A method of manually substituting the few hair amblyseius as predatory mite of feed factory metaplasia production |
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