CN104996363A - Field large-scale propagation method for harmonia axyridis - Google Patents
Field large-scale propagation method for harmonia axyridis Download PDFInfo
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- CN104996363A CN104996363A CN201510362530.5A CN201510362530A CN104996363A CN 104996363 A CN104996363 A CN 104996363A CN 201510362530 A CN201510362530 A CN 201510362530A CN 104996363 A CN104996363 A CN 104996363A
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 241000199694 Harmonia axyridis Species 0.000 title abstract description 9
- 235000013601 eggs Nutrition 0.000 claims abstract description 94
- 230000012447 hatching Effects 0.000 claims abstract description 14
- 241000199695 Harmonia <beetle> Species 0.000 claims description 96
- 235000010749 Vicia faba Nutrition 0.000 claims description 52
- 240000006677 Vicia faba Species 0.000 claims description 52
- 235000002098 Vicia faba var. major Nutrition 0.000 claims description 52
- 241000196324 Embryophyta Species 0.000 claims description 36
- 230000003203 everyday effect Effects 0.000 claims description 33
- 102000002322 Egg Proteins Human genes 0.000 claims description 23
- 108010000912 Egg Proteins Proteins 0.000 claims description 23
- 241000238631 Hexapoda Species 0.000 claims description 23
- 210000004681 ovum Anatomy 0.000 claims description 23
- 238000011081 inoculation Methods 0.000 claims description 20
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 18
- 230000001419 dependent effect Effects 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 11
- 238000009826 distribution Methods 0.000 claims description 10
- 241000382353 Pupa Species 0.000 claims description 9
- 235000013305 food Nutrition 0.000 claims description 7
- 230000000474 nursing effect Effects 0.000 claims description 7
- 239000013589 supplement Substances 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 2
- 230000017448 oviposition Effects 0.000 abstract description 5
- 241000253994 Acyrthosiphon pisum Species 0.000 abstract 1
- 230000018109 developmental process Effects 0.000 description 6
- 238000004321 preservation Methods 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 241001124076 Aphididae Species 0.000 description 3
- 241000255749 Coccinellidae Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000238876 Acari Species 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241001635552 Anthocoridae Species 0.000 description 1
- 241001600407 Aphis <genus> Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 244000299507 Gossypium hirsutum Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 244000130592 Hibiscus syriacus Species 0.000 description 1
- 235000018081 Hibiscus syriacus Nutrition 0.000 description 1
- 244000141359 Malus pumila Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000004920 integrated pest control Methods 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to a field large-scale propagation method for harmonia axyridis. The method is characterized by comprising the following steps: (a) preparing pea aphids; (b) feeding egg laying adults; (c) collecting egg masses; (d) hatching eggs; (e) managing first-instar larvae; (f) inoculating second-instar larvae to fields; (g) carrying out field management on harmonia axyridis; and (h) carrying out field release of harmonia axyridis. The method has the advantages that harmonia axyridis is raised in the fields, so that the artificial culture cost, field transportation cost and field release cost are reduced, and the adaptability of released harmonia axyridis to field climate is improved.
Description
Technical field
The invention belongs to insect green prevention and control field, relate in particular to a kind of harmonia axyridia field and expand numerous method in a large number.
Background technology
Biological control is as one of the important means of integrated pest management; have high efficiency, pollution-free, the feature of environmental protection is conducive to person poultry safety; along with the development of modern agriculture, in widespread attention, and natural enemy insect as a kind of special resource also more and more accept by people.Developed country started to develop natural enemy insect industry from the sixties in last century, at present nearly 150 kinds of natural enemies become commercialized production and selling in the world, main species is oophagous trichogrammae, Cao Ling, Predatory Mites, minute pirate bugs and ladybug etc., and achieves certain Social and economic benef@.
Harmonia axyridia belongs to coleoptera ladybug, is the Natural Enemies of Insect Pests of multiple aphid, aleyrodid, mite, lepidopterous insects ovum and the insect such as low instar larvae and scale insect.Harmonia axyridia originates in Asia, all has distribution, 1916 on China, Mongolia, Korea, Russia, Japan and other places, and California, USA first time introduces harmonia axyridia, and obtains successfully.Through the development of decades, harmonia axyridia has been dispersed throughout each Main Agricultural production area, the whole world, and the Predaceous Coccinellids substituting introducing ground gradually becomes sociales.Harmonia axyridia, compared with other Predaceous Coccinellids, has easy survival, easily breeds, year generation many, the advantages such as predatory number is large are a kind of natural enemy insects of great exploitation potential for its.In China, people carry out biological control by the aphis population of method to tobacco, cotton, apple and peach planting site discharging harmonia axyridia.In developed areas such as Shanghai, a lot of gardens mechanism also utilizes harmonia axyridia to prevent and treat the cotten aphid on the rose of Sharon and the various aphids on booth vegetable.
The field adaptability of harmonia axyridia, depends on artificial room's mass rearing and haul discharges to field, consumes a large amount of human resources and freight.Meanwhile, the harmonia axyridia that indoor feeding obtains lacks good adaptability to the external environment that field is complicated and changeable, produces a large amount of death.
Summary of the invention
The problem to be solved in the present invention is to provide a kind of harmonia axyridia field and expands numerous method in a large number.
Numerous method is expanded in a kind of harmonia axyridia of the present invention field in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: on the broad bean of field planting growth, inoculate acyrthosiphum pisim, add a cover foster worm hut;
B, raise and to lay eggs adult: put in dependent insect cage and lay eggs adult and climb the bean plant of full acyrthosiphum pisim;
C, collection pieces of an egg: after finding pieces of an egg, clip is with the blade of pieces of an egg, puts into collection ovum box;
D, hatching ovum: when the acyrthosiphum pisim quantity in foster worm hut reaches 30 heads/plant; Pieces of an egg are hatched;
E, management first-instar young: hatch after 2-3 days, with little writing brush by first-instar young brush down in cup, hello with a small amount of acyrthosiphum pisim;
After f, Field inoculation second instar larvae: 2-3 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim;
G, field management harmonia axyridia: expulsion natural enemy, artificially disperse a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva climbs to foster worm hut top when starting to pupate, and raises hut door curtain, the harmonia axyridia after emergence can freely be flown out.
Numerous method is expanded in a kind of harmonia axyridia of the present invention field in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: at field planting broad bean, form the little rectangle of 3m*3m, when broad bean grows to 25-35cm, inoculation acyrthosiphum pisim, adds a cover foster worm hut;
B, raise and to lay eggs adult: in dependent insect cage, put into the bean plant that lay eggs adult and acyrthosiphum pisim quantity are 200 heads/plant;
C, collect pieces of an egg: feed every day and lay eggs before adult, check blade, after finding pieces of an egg, clip is with the blade of pieces of an egg, puts into collection ovum box;
D, hatching ovum: when the acyrthosiphum pisim quantity in foster worm hut reaches 30 heads/plant; It is 20-25 degree that pieces of an egg are placed on temperature, and humidity is in the environment of 50%-80%, hatches;
E, management first-instar young: hatch after 2-3 days, with little writing brush by first-instar young brush down in cup, feed with a small amount of acyrthosiphum pisim, and to cover with gauze, rubber band sealing.
After f, Field inoculation second instar larvae: 2-3 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim, density is 3-8 head/plant;
G, field management harmonia axyridia: observe harmonia axyridia growing state every day, expulsion natural enemy, artificially disperses a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva, after field experiences two ages, three ages, four ages, climbs to foster worm hut top and starts to pupate, when ceiling occurring the harmonia axyridia pupa of dense distribution, raise hut door curtain, the harmonia axyridia after emergence can freely be flown out.
Numerous method is expanded in a kind of harmonia axyridia of the present invention field in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: at field planting broad bean, form the little rectangle of 3m*3m, when broad bean grows to 25-35cm, inoculation acyrthosiphum pisim, density is 5 heads/plant, adds a cover foster worm hut simultaneously;
B, raise and to lay eggs adult: in dependent insect cage, put into the bean plant that lay eggs adult and acyrthosiphum pisim quantity are 200 heads/plant, the adult that lays eggs lays eggs on bean plant, the tender point of broad bean that every day, clip climbed full acyrthosiphum pisim puts into dependent insect cage, to supplement the food that fresh acyrthosiphum pisim provides sufficient;
C, collect pieces of an egg: feed every day and lay eggs before adult, check the tender sharp blade of all broad beans and live body Broad Bean Leaves put into the previous day, to find after pieces of an egg with scissors clip, with the blade of pieces of an egg, to be placed in the collection ovum box preserved in 4-6 degree refrigerator;
D, hatching ovum: when the acyrthosiphum pisim quantity in foster worm hut reaches 30 heads/plant; Take out the pieces of an egg preserved in refrigerator, put into pieces of an egg hatching, the condition of controling environment is temperature 20-25 degree, and humidity is that 50%-80% is hatched;
E, management first-instar young: hatch after 2-3 days, with little writing brush by first-instar young brush down in cup, feed with a small amount of acyrthosiphum pisim, and to cover with gauze, rubber band seals, and nursing every day is once;
After f, Field inoculation second instar larvae: 2-3 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim, density is 3-8 head/plant;
G, field management harmonia axyridia: observe harmonia axyridia growing state every day, expulsion natural enemy, artificially disperses a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva, after field experiences two ages, three ages, four ages, climbs to foster worm hut top and starts to pupate, when ceiling occurring the harmonia axyridia pupa of dense distribution, raise hut door curtain, the harmonia axyridia after emergence can freely be flown out.
A kind of harmonia axyridia of the present invention field expand in a large number numerous method support in field worm hut carry out harmonia axyridia field support put, adding the development progress of larva, improve the egg laying amount of adult, is the harmonia axyridia breeding way of science more.
Find in actual production, the field of harmonia axyridia is supported and is put, and only needs the second instar larvae indoor production ladybug, and do not need manually to raise second instar larvae to adult, its breeding expenses reduces about 85%; In addition, by the Imagoes of Harmonia Axyridis of indoor feeding, lack identification in fields, latter one week of release, field survival rate is about 50%, and the harmonia axyridia put and obtain is supported in field, and identification in fields is stronger, latter one week of release, field survival rate still has about 90%, greatly improves field survival rate.
Advantage of the present invention is, raises harmonia axyridia, both decreased the expense of propagating artificially, field freight and field adaptability expense, and too increased the adaptability of the harmonia axyridia after release to field weather in field.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but working of an invention mode is not limited thereto.
Embodiment 1
Numerous method is expanded in a kind of harmonia axyridia of the present invention field in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: at field planting broad bean, form the little rectangle of 3m*3m, when broad bean grows to 25cm, inoculation acyrthosiphum pisim, density is 5 heads/plant, adds a cover foster worm hut simultaneously;
B, raise and to lay eggs adult: in dependent insect cage, put into the bean plant that lay eggs adult and acyrthosiphum pisim quantity are 200 heads/plant, the adult that lays eggs lays eggs on bean plant, and every day, clip climbed the tender point of broad bean of full acyrthosiphum pisim, to supplement the food that fresh acyrthosiphum pisim provides sufficient;
C, collect pieces of an egg: feed every day and lay eggs before adult, check the tender sharp blade of all broad beans and live body Broad Bean Leaves put into the previous day, to find after pieces of an egg, with the blade of scissors clip with pieces of an egg, to be placed in the collection ovum box of 4 degree preservations in refrigerator;
D, hatching ovum: when the acyrthosiphum pisim quantity of supporting in worm hut reaches 30 heads/plant, take out the pieces of an egg preserved in refrigerator, being placed on temperature is 20 degree, and humidity is in the environment of 50%, hatches;
E, management first-instar young: hatch after 2 days, with little writing brush by first-instar young brush down in cup, feed with a small amount of acyrthosiphum pisim, and to cover with gauze, rubber band seals, and nursing every day is once;
F, Field inoculation second instar larvae: after 2 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim, density is 3 heads/plant;
G, field management harmonia axyridia: observe harmonia axyridia growing state every day, expulsion natural enemy, artificially disperses a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva, after field experiences two ages, three ages, four ages, climbs to foster worm hut top and starts to pupate, when ceiling occurring the harmonia axyridia pupa of dense distribution, raise hut door curtain, the harmonia axyridia after emergence can freely be flown out.
Embodiment 2
Numerous method is expanded in a kind of harmonia axyridia of the present invention field in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: at field planting broad bean, form the little rectangle of 3m*3m, when broad bean grows to 35cm, inoculation acyrthosiphum pisim, density is 5 heads/plant, adds a cover foster worm hut simultaneously;
B, raise and to lay eggs adult: in dependent insect cage, put into the bean plant that lay eggs adult and acyrthosiphum pisim quantity are 200 heads/plant, the adult that lays eggs lays eggs on bean plant, and every day, clip climbed the tender point of broad bean of full acyrthosiphum pisim, to supplement the food that fresh acyrthosiphum pisim provides sufficient;
C, collect pieces of an egg: feed every day and lay eggs before adult, check the tender sharp blade of all broad beans and live body Broad Bean Leaves put into the previous day, to find after pieces of an egg, with the blade of scissors clip with pieces of an egg, to be placed in the collection ovum box of 6 degree preservations in refrigerator;
D, hatching ovum: when the acyrthosiphum pisim quantity of supporting in worm hut reaches 30 heads/plant, take out the pieces of an egg preserved in refrigerator, being placed on temperature is 25 degree, and humidity is in the environment of 80%, hatches;
E, management first-instar young: hatch after 3 days, with little writing brush by first-instar young brush down in cup, feed with a small amount of acyrthosiphum pisim, and to cover with gauze, rubber band seals, and nursing every day is once;
F, Field inoculation second instar larvae: after 3 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim, density is 3-8 head/plant;
G, field management harmonia axyridia: observe harmonia axyridia growing state every day, expulsion natural enemy, artificially disperses a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva, after field experiences two ages, three ages, four ages, climbs to foster worm hut top and starts to pupate, when ceiling occurring the harmonia axyridia pupa of dense distribution, raise hut door curtain, the harmonia axyridia after emergence can freely be flown out.
Embodiment 3
Numerous method is expanded in a kind of harmonia axyridia of the present invention field in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: at field planting broad bean, form the little rectangle of 3m*3m, when broad bean grows to 30cm, inoculation acyrthosiphum pisim, density is 5 heads/plant, adds a cover foster worm hut simultaneously;
B, raise and to lay eggs adult: in dependent insect cage, put into the bean plant that lay eggs adult and acyrthosiphum pisim quantity are 200 heads/plant, the adult that lays eggs lays eggs on bean plant, and every day, clip climbed the tender point of broad bean of full acyrthosiphum pisim, to supplement the food that fresh acyrthosiphum pisim provides sufficient;
C, collect pieces of an egg: feed every day and lay eggs before adult, check the tender sharp blade of all broad beans and live body Broad Bean Leaves put into the previous day, to find after pieces of an egg, with the blade of scissors clip with pieces of an egg, to be placed in the collection ovum box of 5 degree preservations in refrigerator;
D, hatching ovum: when the acyrthosiphum pisim quantity of supporting in worm hut reaches 30 heads/plant, take out the pieces of an egg preserved in refrigerator, being placed on temperature is 22 degree, and humidity is in the environment of 70%, hatches;
E, management first-instar young: hatch after 3 days, with little writing brush by first-instar young brush down in cup, feed with a small amount of acyrthosiphum pisim, and to cover with gauze, rubber band seals, and nursing every day is once;
F, Field inoculation second instar larvae: after 2 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim, density is 5 heads/plant;
G, field management harmonia axyridia: observe harmonia axyridia growing state every day, expulsion natural enemy, artificially disperses a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva, after field experiences two ages, three ages, four ages, climbs to foster worm hut top and starts to pupate, when ceiling occurring the harmonia axyridia pupa of dense distribution, raise hut door curtain, the harmonia axyridia after emergence can freely be flown out.
Embodiment 4
Numerous method is expanded in a kind of harmonia axyridia field according to claim 1 in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: at field planting broad bean, form the little rectangle of 3m*3m, when broad bean grows to 25-35cm, inoculation acyrthosiphum pisim, density is 5 heads/plant, adds a cover foster worm hut simultaneously;
B, raise and to lay eggs adult: in dependent insect cage, put into the bean plant that lay eggs adult and acyrthosiphum pisim quantity are 200 heads/plant, the adult that lays eggs lays eggs on bean plant, the tender point of broad bean that every day, clip climbed full acyrthosiphum pisim puts into dependent insect cage, to supplement the food that fresh acyrthosiphum pisim provides sufficient;
C, collect pieces of an egg: feed every day and lay eggs before adult, check the tender sharp blade of all broad beans and live body Broad Bean Leaves put into the previous day, to find after pieces of an egg with scissors clip, with the blade of pieces of an egg, to be placed in the collection ovum box preserved in 4-6 degree refrigerator;
D, hatching ovum: when the acyrthosiphum pisim quantity in foster worm hut reaches 30 heads/plant, take out the pieces of an egg preserved in refrigerator, put into pieces of an egg hatching, the condition of controling environment is temperature 20-25 degree, and humidity is that 50%-80% is hatched;
E, management first-instar young: hatch after 2-3 days, with little writing brush by first-instar young brush down in cup, feed with a small amount of acyrthosiphum pisim, and to cover with gauze, rubber band seals, and nursing every day is once;
After f, Field inoculation second instar larvae: 2-3 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim, density is 3-8 head/plant;
G, field management harmonia axyridia: observe harmonia axyridia growing state every day, expulsion natural enemy, artificially disperses a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva, after field experiences two ages, three ages, four ages, climbs to foster worm hut top and starts to pupate, when ceiling occurring the harmonia axyridia pupa of dense distribution, raise hut door curtain, the harmonia axyridia after emergence can freely be flown out.
Embodiment 5
Numerous method is expanded in a kind of harmonia axyridia of the present invention field in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: at field planting broad bean, form the little rectangle of 3m*3m, when broad bean grows to 30cm, inoculation acyrthosiphum pisim, density is 5 heads/plant, adds a cover foster worm hut simultaneously;
B, raise and to lay eggs adult: in dependent insect cage, put into the bean plant that lay eggs adult and acyrthosiphum pisim quantity are 200 heads/plant, the adult that lays eggs lays eggs on bean plant, and every day, clip climbed the tender point of broad bean of full acyrthosiphum pisim, to supplement the food that fresh acyrthosiphum pisim provides sufficient;
C, collect pieces of an egg: feed every day and lay eggs before adult, check the tender sharp blade of all broad beans and live body Broad Bean Leaves put into the previous day, to find after pieces of an egg, with the blade of scissors clip with pieces of an egg, to be placed in the collection ovum box of 5 degree preservations in refrigerator;
D, hatching ovum: when the acyrthosiphum pisim quantity of supporting in worm hut reaches 30 heads/plant, take out the pieces of an egg preserved in refrigerator, being placed on temperature is 22 degree, and humidity is in the environment of 70%, hatches;
E, management first-instar young: hatch after 3 days, with little writing brush by first-instar young brush down in cup, feed with a small amount of acyrthosiphum pisim, and to cover with gauze, rubber band seals, and nursing every day is once;
F, Field inoculation second instar larvae: after 2 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim, density is 5 heads/plant;
G, field management harmonia axyridia: observe harmonia axyridia growing state every day, expulsion natural enemy, artificially disperses a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva, after field experiences two ages, three ages, four ages, climbs to foster worm hut top and starts to pupate, when ceiling occurring the harmonia axyridia pupa of dense distribution, raise hut door curtain, the harmonia axyridia after emergence can freely be flown out.
The contrast situation of numerous method is expanded in a kind of harmonia axyridia of the present invention field in a large number, getting the harmonia axyridia 20 that new decortication is second instar larvae, getting 10 and be seeded in field and support in worm hut, raise in indoor insect box for other 10, every day, record patted equal development duration everywhere, and repeated 5 times.After Larva Harmonia Axyridis turns into adult, choose female, male worm respectively each 10 right, often pair is placed in dependent insect cage and raises separately, feeds with enough acyrthosiphum pisims, the female average daily egg laying amount of list of the adult obtained under comparing two kinds of yard feeding pattern.
The results are shown in following table:
Process
Result shows, field is supported the Larva Harmonia Axyridis development duration grown in worm hut and is obviously shortened, its single female egg laying amount also obviously increases, therefore, support in field worm hut carry out harmonia axyridia field support put, adding the development progress of larva, improve the egg laying amount of adult, is the harmonia axyridia breeding way of science more.
Claims (3)
1. a numerous method is expanded in harmonia axyridia field in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: on the broad bean of field planting growth, inoculate acyrthosiphum pisim, add a cover foster worm hut;
B, raise and to lay eggs adult: put in dependent insect cage and lay eggs adult and climb the bean plant of full acyrthosiphum pisim;
C, collection pieces of an egg: after finding pieces of an egg, clip is with the blade of pieces of an egg, puts into collection ovum box;
D, hatching ovum: when the acyrthosiphum pisim quantity in foster worm hut reaches 30 heads/plant; Pieces of an egg are hatched;
E, management first-instar young: hatch after 2-3 days, with little writing brush by first-instar young brush down in cup, hello with a small amount of acyrthosiphum pisim;
After f, Field inoculation second instar larvae: 2-3 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim;
G, field management harmonia axyridia: expulsion natural enemy, artificially disperse a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva climbs to foster worm hut top when starting to pupate, and raises hut door curtain, the harmonia axyridia after emergence can freely be flown out.
2. numerous method is expanded in a kind of harmonia axyridia field according to claim 1 in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: at field planting broad bean, form the little rectangle of 3m*3m, when broad bean grows to 25-35cm, inoculation acyrthosiphum pisim, adds a cover foster worm hut;
B, raise and to lay eggs adult: in dependent insect cage, put into the bean plant that lay eggs adult and acyrthosiphum pisim quantity are 200 heads/plant;
C, collect pieces of an egg: feed every day and lay eggs before adult, check blade, after finding pieces of an egg, clip is with the blade of pieces of an egg, puts into collection ovum box;
D, hatching ovum: when the acyrthosiphum pisim quantity in foster worm hut reaches 30 heads/plant; It is 20-25 degree that pieces of an egg are placed on temperature, and humidity is in the environment of 50%-80%, hatches;
E, management first-instar young: hatch after 2-3 days, with little writing brush by first-instar young brush down in cup, feed with a small amount of acyrthosiphum pisim, and to cover with gauze, rubber band sealing.
After f, Field inoculation second instar larvae: 2-3 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim, density is 3-8 head/plant;
G, field management harmonia axyridia: observe harmonia axyridia growing state every day, expulsion natural enemy, artificially disperses a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva, after field experiences two ages, three ages, four ages, climbs to foster worm hut top and starts to pupate, when ceiling occurring the harmonia axyridia pupa of dense distribution, raise hut door curtain, the harmonia axyridia after emergence can freely be flown out.
3. numerous method is expanded in a kind of harmonia axyridia field according to claim 1 in a large number, it is characterized in that comprising the following steps:
A, preparation acyrthosiphum pisim: at field planting broad bean, form the little rectangle of 3m*3m, when broad bean grows to 25-35cm, inoculation acyrthosiphum pisim, density is 5 heads/plant, adds a cover foster worm hut simultaneously;
B, raise and to lay eggs adult: in dependent insect cage, put into the bean plant that lay eggs adult and acyrthosiphum pisim quantity are 200 heads/plant, the adult that lays eggs lays eggs on bean plant, the tender point of broad bean that every day, clip climbed full acyrthosiphum pisim puts into dependent insect cage, to supplement the food that fresh acyrthosiphum pisim provides sufficient;
C, collect pieces of an egg: feed every day and lay eggs before adult, check the tender sharp blade of all broad beans and live body Broad Bean Leaves put into the previous day, to find after pieces of an egg with scissors clip, with the blade of pieces of an egg, to be placed in the collection ovum box preserved in 4-6 degree refrigerator;
D, hatching ovum: when the acyrthosiphum pisim quantity in foster worm hut reaches 30 heads/plant, take out the pieces of an egg preserved in refrigerator; Pieces of an egg are hatched, and the condition of controling environment is temperature 20-25 degree, and humidity is that 50%-80% is hatched;
E, management first-instar young: hatch after 2-3 days, with little writing brush by first-instar young brush down in cup, feed with a small amount of acyrthosiphum pisim, and to cover with gauze, rubber band seals, and nursing every day is once;
After f, Field inoculation second instar larvae: 2-3 days, get second instar larvae with little writing brush type brush and be inoculated in field and support in worm hut growth and have on the tender point of the broad bean of a large amount of acyrthosiphum pisim, density is 3-8 head/plant;
G, field management harmonia axyridia: observe harmonia axyridia growing state every day, expulsion natural enemy, artificially disperses a small amount of harmonia axyridia concentrated in together;
H, field adaptability harmonia axyridia: larva, after field experiences two ages, three ages, four ages, climbs to foster worm hut top and starts to pupate, when ceiling occurring the harmonia axyridia pupa of dense distribution, raise hut door curtain, the harmonia axyridia after emergence can freely be flown out.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106879405A (en) * | 2017-02-17 | 2017-06-23 | 浙江省柑桔研究所 | The method for preventing and treating citrus psylla using artificial release harmonia axyridia |
| CN109362654A (en) * | 2018-12-25 | 2019-02-22 | 甘肃农业大学 | A kind of Larva Harmonia Axyridis breeding apparatus and its method for breeding |
| CN111264476A (en) * | 2020-03-12 | 2020-06-12 | 四川省农业科学院经济作物育种栽培研究所 | Predatory ladybug egg mass collecting and collecting method and device |
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| CN102948401A (en) * | 2012-11-01 | 2013-03-06 | 湖北省烟草公司恩施州公司 | artificial mass propagation method of harmonia axyridis |
| CN103355259A (en) * | 2013-07-30 | 2013-10-23 | 天津拓华生物科技有限责任公司 | Method for artificially breeding harmonia axyridis |
| CN104585137A (en) * | 2014-12-31 | 2015-05-06 | 红塔烟草(集团)有限责任公司 | Method for preventing and curing tobacco pests by ladybird breeding via fennel aphids in field |
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| CN102948401A (en) * | 2012-11-01 | 2013-03-06 | 湖北省烟草公司恩施州公司 | artificial mass propagation method of harmonia axyridis |
| CN103355259A (en) * | 2013-07-30 | 2013-10-23 | 天津拓华生物科技有限责任公司 | Method for artificially breeding harmonia axyridis |
| CN104585137A (en) * | 2014-12-31 | 2015-05-06 | 红塔烟草(集团)有限责任公司 | Method for preventing and curing tobacco pests by ladybird breeding via fennel aphids in field |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106879405A (en) * | 2017-02-17 | 2017-06-23 | 浙江省柑桔研究所 | The method for preventing and treating citrus psylla using artificial release harmonia axyridia |
| CN106879405B (en) * | 2017-02-17 | 2020-09-15 | 浙江省柑桔研究所 | Method for controlling diaphorina citri by artificially releasing harmonia axyridis |
| CN109362654A (en) * | 2018-12-25 | 2019-02-22 | 甘肃农业大学 | A kind of Larva Harmonia Axyridis breeding apparatus and its method for breeding |
| CN111264476A (en) * | 2020-03-12 | 2020-06-12 | 四川省农业科学院经济作物育种栽培研究所 | Predatory ladybug egg mass collecting and collecting method and device |
| CN111264476B (en) * | 2020-03-12 | 2024-06-07 | 四川省农业科学院经济作物育种栽培研究所 | Predatory ladybug egg block collecting method and device |
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