CN104041462A - Method for regulating and controlling diapause of microplitis syntretomorpha - Google Patents
Method for regulating and controlling diapause of microplitis syntretomorpha Download PDFInfo
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- CN104041462A CN104041462A CN201410314662.6A CN201410314662A CN104041462A CN 104041462 A CN104041462 A CN 104041462A CN 201410314662 A CN201410314662 A CN 201410314662A CN 104041462 A CN104041462 A CN 104041462A
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Abstract
The invention provides a method for regulating and controlling diapause of microplitis syntretomorpha. The method includes the steps of diapause induction, diapause storage and diapause relieving. In the step of diapause induction, prodenia litura where microplitis syntretomorpha is parasitized for 1 day to 3 days is independently subpackaged in flat-bottom test tubes, the test tubes are placed under the condition with the temperature of 18+/-1 DEG C, the relative humidity of 70+/-10% and the photoperiod of L10:D14 for induction, and microplitis cocoons in the diapause state are obtained after host larvae come out of the cocoons. In the diapause storage step, wet fine sand is laid on the diapause cocoons after the host larvae are removed, the diapause cocoons are transferred to the dark environment with the temperature of 6 DEG C to be stored. In the diapause relieving step, the diapause cocoons are placed under the condition with the temperature of 27+/-2 DEG C, the humidity of 70+/-10% and the photoperiod of L14:D10, and eclosion microplitis syntretomorpha is obtained in about 5 days. The diapause rate of microplitis syntretomorpha induced through the method can reach more than 98%, the diapause cocoons are stored for 3 months to 5 months under the condition of 6 DEG C, and the eclosion microplitis syntretomorpha does not degenerate in the aspects of the egg laying amount, the life and other biological indicators. By means of the method, the retention cycle of microplitis syntretomorpha can be effectively prolonged by 4 times to 5 times, and the reference basis is provided for regulating and controlling development of microplitis syntretomorpha.
Description
Technical field
The invention belongs to the biological prevention field of agricultural insect pests control, relate to the method for a kind of Si Shi of regulation and control Microplitis Sp diapause.
Background technology
Prodenia litura is a kind of universal omnivorousness, fulminating pest, this worm can endanger 99 sections, more than 290 Plants, generation overlap is serious, wildness in recent years causes harm on China's various vegetables and economic crops, newly hatched larvae has gluttony, can be by the flower of surface crops, blade, fruit, even stem stalk is cleared off, its host is many, generation in a year is from generation to generation many, allow the harm increasing year by year of prodenia litura, add to again a large amount of frequent use of chemical pesticide, the pesticide resistance of prodenia litura is strengthened year by year, can not get effectively controlling.Therefore, on producing, in the urgent need to improving and promote the anti-control techniques of insect, utilize biological control to carry out insect protected pest controlling, can well avoid the shortcoming of traditional chemical control.
Utilizing the pest natural enemies such as parasitic wasp to carry out pest controlling to insect is a kind of important biological control of insect pests means.Parasitic wasp is the important natural enemy insect of prodenia litura, and honeybee produces ovum in pin main body, and after the hatching of honeybee ovum, larva of bee draws nutrition in pin main body, makes host can not complete normal life and goes through the phase final dead, thereby reach the object of prevention and control insect.At present, people have developed various pests natural enemy commodity, in agricultural production, insect can be controlled at below economic threshold, and control efficiency is very remarkable.The for example natural enemy of primary pest corn borer on corn---Trichogramma Ostriniae can very effectively be controlled corn borer to the causing harm of corn, and improves China's grain yield.But, also not yet there is remarkable achievement for the natural enemy product of this throughout the year multiple insect of prodenia litura.
Because natural enemy self grows, host is had to high dependency, therefore, how on the basis of artificial propagation natural enemy, growing of natural enemy population carried out to Effective Regulation, thereby in adaptation agricultural production, population fluctuation and the Harm of insect are the technical barriers that natural enemy product development is primarily captured.At occurring in nature, the breeding of natural enemy need to rely on its natural host, and in the process of artificial breeding pest natural enemy, also should fully take into account this characteristic, effectively adjusts the Population Growth relation between host and natural enemy.And the regulation and control of realization to natural enemy insect development progress, can utilize insect to be subject to external environment induction to reach this characteristic of diapause, by understanding the development characteristics of natural enemy insect, natural enemy insect is carried out to diapause regulation and control, at a large amount of timely termination of diapause in season that occur of insect, natural enemy insect is thrown in to field, cooperatively interact with parasitic wasp population under natural conditions, reach the object of insect protected; The natural enemy insect of means by diapause storage is simultaneously also a kind of desirable long-term protecting method, can solve natural enemy insect survival period shorter, expands the problem that numerous cycle grows, and extends in the prevention and control action time in field, improves control of insect efficiency.
Summary of the invention
Technical problem to be solved by this invention is: for above-mentioned prior art blank, taking prodenia litura solitary parasitism cocoon honeybee Si Shi Microplitis Sp as example, the method of a kind of Si Shi of regulation and control Microplitis Sp diapause is provided, by this method, Si Shi Microplitis Sp is carried out to diapause regulation and control, realize preservation and the rejuvenation of the Si Shi Microplitis Sp population of indoor propagation, produce the upper requirement to insect prevention and control to reach.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of regulate and control the method for Si Shi Microplitis Sp diapause, the method step is as follows:
A. diapause induction: comprise two steps of the acquisition in worm source and the induction of diapause;
The acquisition in worm source: prodenia litura is put into flat based tubes together with vegetable leaf, access than the ratio of 1:10 the queen bee that mating is good with host according to honeybee, after meeting honeybee 2h, queen bee is shifted out, put into the test tube that is ready to prodenia litura and vegetable leaf and carry out next round breeding, until the death of honeybee;
The induction of diapause: after queen bee shifts out, the above-mentioned Spodoptera litura larvae flat based tubes of taking over honeybee is carried out to independent packing, raise taking artificial feed and be placed in 27 ± 2 DEG C, photoperiod after the climatic cabinate of L14:D10 is fed 1d, 2d or 3d, proceed in batches 18 ± 1 DEG C, humidity 70 ± 10%, photoperiod is that the climatic cabinate of L10:D14 carries out diapause induction, and the state that grows of routine observation host larva obtains the cocoon of diapause status after host larva goes out cocoon;
B. diapause stores: the diapause cocoon of step a gained is proceeded in the flat based tubes that is covered with moist fine sand, be placed in the dark storage of 6 DEG C of environment; Spraying water to keep humidity every 5 days to fine sand is 70-80%;
C. diapause is removed: be that 25-29 DEG C, humidity are under the condition of 60-80%, photoperiod L14:D10 by the diapause cocoon of storage in temperature, go through 4-6 days, and bee eclosion, diapause status is removed, and obtains Si Shi Microplitis Sp.
Si Shi Microplitis Sp is one of Natural Enemies of prodenia litura, can effectively control the harm of prodenia litura to crop, promotes agricultural product quality, and its ecological benefits and economic benefit are all comparatively remarkable.Adopt method of the present invention, form with diapause cocoon can be preserved 3-5 month at 6 DEG C, degenerating does not appear in the honeybee after emergence in the biological indicator such as egg laying amount, life-span, remove the time that can control more exactly bee eclosion by diapause, can effectively extend honeybee kind retention cycle 4-5 doubly, be conducive to the production of artificial breeding honeybee.Generally speaking, utilize this method, can realize the conservation storage of Si Shi Microplitis Sp, thereby enriched the means of prodenia litura control, and then for the regulation and control of growing of pest natural enemy provide theoretical foundation, pest biological control is significant.
Embodiment
With specific embodiment, the present invention is further elaborated below.
For examination worm source
Prodenia litura and Si Shi Microplitis Sp are the population of Agricultural University Of Hunan's institute of viruses's artificial propagation, and condition of culture is 27 ± 2 DEG C, photoperiod L14:D10, relative moisture 70 ± 10%.Prodenia litura breeds with artificial feed.Si Shi Microplitis Sp adult, using 30% hydromel as nutritional supplementation, provides a large amount of 2-3 prodenia litura in age hosts to allow it carry out egg reproduction.
Test apparatus and material
Temperature and photoperiod carry out in climatic cabinate the experiment of Si Shi Microplitis Sp diapause induction, set two different environment, the breeding of insect population is carried out under 27 ± 2 DEG C, photoperiod L14:D10, relative moisture 70 ± 10% conditions, and diapause induction is carried out under 18 ± 1 DEG C, photoperiod L10:D14, relative moisture 70 ± 10% conditions.All the other materials have flat based tubes (2 × 10cm), gauze, cotton, elastic tape, 30% hydromel, artificial feed and wet sand etc.
Embodiment 1
1) experience the acquisition of diapause signal worm state: comprise that host cultivates, the cultivation of honeybee, connect three processes of honeybee.
Host cultivation: in climatic cabinate (27 ± 2 DEG C, L14:D10, relative moisture 70 ± 10%), prodenia litura connects generation and raises.Larva is raised with artificial feed, to six age mature larvas, larva is proceeded to the independent raising of flat based tubes to pupating.After adult eclosion, raise with 10% hydromel, ovum is collected in male and female pairings, and pieces of an egg are transferred to and on artificial feed, treat its hatching.
The cultivation of honeybee: taking 2-3 prodenia litura in age as host, prodenia litura is moved in flat based tubes together with vegetable leaf, put in test tube bottom the cotton balls that is moistened with 30% hydromel, access the queen bee of mating 12h with host than the ratio of 1:10 according to honeybee, connect after honeybee 2h, shift out queen bee and carry out next round and connect honeybee; Prodenia litura in flat based tubes after queen bee shifts out carries out packing raising, and its state that grows of routine observation, host larva goes out after cocoon, collect parasitic wasp cocoon, treat that parasitic wasp sprouts wings, nutritional supplementation using 30% hydromel as bee imago, by after male and female mating 12h, shifting out queen bee, to carry out follow-on expansion numerous.
Connect honeybee: a large amount of by parasitic host for obtaining, get the prodenia litura just casting off a skin to 3 ages some, with vegetable leaf (2cm
2) put into flat based tubes, 10 of every pipes put in test tube bottom the cotton balls that is moistened with 30% hydromel, access a queen bee of mating 12h, after 2h, queen bee are shifted out.This process repeats 20 honeybees.
2) diapause induction: after queen bee shifts out, prodenia litura is taken out separately and divides and install in the flat based tubes that is loaded with feed, put into the climatic cabinate of 27 ± 2 DEG C of temperature, relative moisture 70 ± 10%, photoperiod L14:D10.First host moves into (climatic cabinate that 18 ± 1 DEG C of temperature, humidity 70 ± 10%, photoperiod are L10:D14) in diapause condition after by parasitic 1d, in like manner, second batch, the 3rd crowd of host are respectively at being moved into after parasitic 2d, 3d in diapause condition, to obtain the Si Shi Microplitis Sp cocoon of diapause status.Diapause cocoon surface is light green color, is obviously different from the light brown of non-diapause cocoon.
3) storage requirement: by above-mentioned 2) in the diapause cocoon that obtains proceed in the flat based tubes that is covered with moist fine sand, be positioned over dark storage in the refrigerating chamber of 6 DEG C, spray a small amount of water to keep humidity as 70-80% every 5d to fine sand.
4) diapause is removed: to the Si Shi Microplitis Sp of diapause state, stimulate in conjunction with photoperiodic signal by temperature, termination of diapause, enters development process, and concrete measure is as follows:
The diapause state Si Shi Microplitis Sp that 6 DEG C of low temperature are preserved is placed in 27 ± 2 DEG C, the climatic cabinate of humidity 70 ± 10%, photoperiod L14:D10 again.Every day, timing was observed, about 5d left and right, and bee eclosion, diapause status is removed, and obtains the Si Shi Microplitis Sp of sprouting wings.
5) detection of diapause honeybee biological indicator
Get above-mentioned 4) the middle honeybee sprouting wings, raise with 30% hydromel, make male and female pairing 12h, again queen bee is moved into the flat based tubes that provides host and vegetable leaf, connect honeybee with host than the ratio of 1:10 according to honeybee, every 2h, queen bee is shifted out, packing host plant for rearing, carry out next round and connect honeybee, and so forth, until the death of queen bee is observed host and is gone out cocoon situation, record out cocoon number, and record honeybee survival number of days.This experiment is established contrast for non-diapause honeybee, and all the other conditions are identical, and it the results are shown in following table 1 and table 2.
Different diapause and the emergence of growing number of days honeybee of table 1
Note: will be moved in diapause environment by parasitic host after the growth number of days before immigration diapause environment represents to connect the 1st, 2 after honeybee, 3d.It is the surface color of cocoon that successful discrimination standard is induced in diapause, and light green color is diapause cocoon, is obviously different from the light brown of non-diapause cocoon.Identical letter representation there was no significant difference (p < 0.05, LSD).
The detection of table 2 diapause honeybee biological indicator
Note: will be moved in diapause environment by parasitic host after the growth number of days before immigration diapause environment represents to connect the 1st, 2 after honeybee, 3d.Identical letter representation there was no significant difference (p < 0.05, LSD).
Found out by upper table 2, although it is relatively low to move into the larva of bee diapause inductivity of the growth 3d before diapause environment, but its life-span and spawning rate are without big-difference too, can find that by contrasting non-diapause honeybee relevant data honeybee life-span, egg laying amount after diapause are not all affected, prove to there is feasibility with the diapause of the method regulation and control honeybee.
Claims (2)
1. a method that regulates and controls the diapause of Si Shi Microplitis Sp, is characterized in that, the method step is as follows:
A. diapause induction: after independent the Spodoptera litura larvae of being surveyed the parasitic 1d-3d of ditch cocoon honeybee by Si Shi point is installed in the flat based tubes that is loaded with feed, be placed under temperature 17-19 DEG C, the condition of humidity 60-80%, photoperiod L10:D14 and induce, after host larva goes out cocoon, obtain the honeybee cocoon of diapause status;
B. diapause stores: the diapause cocoon of step a gained is proceeded in the flat based tubes that is covered with moist fine sand, be placed in the dark storage of 6 DEG C of environment;
C. diapause is removed: be that 25-29 DEG C, humidity are under the condition of 60-80%, photoperiod L14:D10 by the diapause cocoon of storage in temperature, go through 4-6 days, and bee eclosion, diapause status is removed, and obtains Si Shi Microplitis Sp.
As claimed in claim 1 a kind of regulate and control the method for Si Shi Microplitis Sp diapause, it is characterized in that, after diapause cocoon proceeds to tiling and have in the flat based tubes of moist fine sand in step b, sprayed water to keep 70-80% humidity every 5 days to fine sand.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304193A (en) * | 2014-10-28 | 2015-01-28 | 河北省农林科学院旱作农业研究所 | Aphidoletes aphidimyza diapause induction method and application thereof to production of aphidoletes aphidimyza |
| CN105766804A (en) * | 2016-03-18 | 2016-07-20 | 中南林业科技大学 | Artificial breeding method of cotesia chilonis matsumura |
| CN105794727A (en) * | 2016-03-24 | 2016-07-27 | 镇远县醉美果业有限公司 | Osmia cocoon breaking method |
| CN109874752A (en) * | 2019-04-03 | 2019-06-14 | 中国农业科学院植物保护研究所 | A kind of diapause regulation of the endoparasitoid Microcentrus cingulum and storage method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2158506C1 (en) * | 1999-05-19 | 2000-11-10 | Всероссийский научно-исследовательский институт защиты растений | Method of rearing midge (aphidoletes aphidimyza rond) |
| CN1273027A (en) * | 2000-06-15 | 2000-11-15 | 河北省农林科学院植物保护研究所 | Artificial reproduction method for Microplitis mediator (Haliday) |
| CN101326902A (en) * | 2008-07-04 | 2008-12-24 | 衡水田益生防有限责任公司 | Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method |
| CN101720724A (en) * | 2009-12-04 | 2010-06-09 | 沈阳农业大学 | Long-term storage method of bee species of artificially diapaused and induced Chouioia cunea |
| CN102640733A (en) * | 2012-05-03 | 2012-08-22 | 中国农业科学院植物保护研究所 | Method for regulating diapause of aphidius gifuensis ashmead |
| CN103141443A (en) * | 2013-03-08 | 2013-06-12 | 中国农业科学院草原研究所 | Method for regulating and controlling diapause of agryponflexorius |
| CN103734095A (en) * | 2014-01-17 | 2014-04-23 | 湖南农业大学 | Indoor artificial reproduction method of monoparasitism apanteles ruficrus haliday |
-
2014
- 2014-07-03 CN CN201410314662.6A patent/CN104041462A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2158506C1 (en) * | 1999-05-19 | 2000-11-10 | Всероссийский научно-исследовательский институт защиты растений | Method of rearing midge (aphidoletes aphidimyza rond) |
| CN1273027A (en) * | 2000-06-15 | 2000-11-15 | 河北省农林科学院植物保护研究所 | Artificial reproduction method for Microplitis mediator (Haliday) |
| CN101326902A (en) * | 2008-07-04 | 2008-12-24 | 衡水田益生防有限责任公司 | Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method |
| CN101720724A (en) * | 2009-12-04 | 2010-06-09 | 沈阳农业大学 | Long-term storage method of bee species of artificially diapaused and induced Chouioia cunea |
| CN102640733A (en) * | 2012-05-03 | 2012-08-22 | 中国农业科学院植物保护研究所 | Method for regulating diapause of aphidius gifuensis ashmead |
| CN103141443A (en) * | 2013-03-08 | 2013-06-12 | 中国农业科学院草原研究所 | Method for regulating and controlling diapause of agryponflexorius |
| CN103734095A (en) * | 2014-01-17 | 2014-04-23 | 湖南农业大学 | Indoor artificial reproduction method of monoparasitism apanteles ruficrus haliday |
Non-Patent Citations (1)
| Title |
|---|
| 李玉艳等: "茧蜂滞育的研究进展", 《昆虫学报》, vol. 53, no. 10, 20 October 2010 (2010-10-20), pages 1167 - 1178 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304193A (en) * | 2014-10-28 | 2015-01-28 | 河北省农林科学院旱作农业研究所 | Aphidoletes aphidimyza diapause induction method and application thereof to production of aphidoletes aphidimyza |
| CN105766804A (en) * | 2016-03-18 | 2016-07-20 | 中南林业科技大学 | Artificial breeding method of cotesia chilonis matsumura |
| CN105794727A (en) * | 2016-03-24 | 2016-07-27 | 镇远县醉美果业有限公司 | Osmia cocoon breaking method |
| CN105794727B (en) * | 2016-03-24 | 2018-06-01 | 镇远县醉美果业有限公司 | A kind of wall bee goes out cocoon method |
| CN109874752A (en) * | 2019-04-03 | 2019-06-14 | 中国农业科学院植物保护研究所 | A kind of diapause regulation of the endoparasitoid Microcentrus cingulum and storage method |
| CN109874752B (en) * | 2019-04-03 | 2021-04-09 | 中国农业科学院植物保护研究所 | Diapause regulation and storage method for waistband long body hornets |
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Application publication date: 20140917 |