CN104304193A - Aphidoletes aphidimyza diapause induction method and application thereof to production of aphidoletes aphidimyza - Google Patents
Aphidoletes aphidimyza diapause induction method and application thereof to production of aphidoletes aphidimyza Download PDFInfo
- Publication number
- CN104304193A CN104304193A CN201410586984.6A CN201410586984A CN104304193A CN 104304193 A CN104304193 A CN 104304193A CN 201410586984 A CN201410586984 A CN 201410586984A CN 104304193 A CN104304193 A CN 104304193A
- Authority
- CN
- China
- Prior art keywords
- diapause
- aphid cecidomyiia
- aphidoletes aphidimyza
- food aphid
- cecidomyiia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 230000005058 diapause Effects 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000004519 manufacturing process Methods 0.000 title abstract description 4
- 241000372435 Aphidoletes aphidimyza Species 0.000 title abstract 10
- 230000006698 induction Effects 0.000 title abstract 3
- 238000003860 storage Methods 0.000 claims abstract description 10
- 241001124076 Aphididae Species 0.000 claims description 60
- 235000013305 food Nutrition 0.000 claims description 50
- 241000382353 Pupa Species 0.000 claims description 18
- 102000002322 Egg Proteins Human genes 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 7
- 230000032669 eclosion Effects 0.000 abstract description 6
- 235000013601 eggs Nutrition 0.000 abstract description 4
- 230000001850 reproductive effect Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 description 5
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 241000209140 Triticum Species 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- CVOFKRWYWCSDMA-UHFFFAOYSA-N 2-chloro-n-(2,6-diethylphenyl)-n-(methoxymethyl)acetamide;2,6-dinitro-n,n-dipropyl-4-(trifluoromethyl)aniline Chemical compound CCC1=CC=CC(CC)=C1N(COC)C(=O)CCl.CCCN(CCC)C1=C([N+]([O-])=O)C=C(C(F)(F)F)C=C1[N+]([O-])=O CVOFKRWYWCSDMA-UHFFFAOYSA-N 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 241000167882 Rhopalosiphum maidis Species 0.000 description 1
- 241001481659 Syrphidae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses an aphidoletes aphidimyza diapause induction method and application thereof to production of aphidoletes aphidimyza. Diapause aphidoletes aphidimyza pupae are obtained by putting aphidoletes aphidimyza eggs at the day temperature of 20-26 DEG C at the night temperature of 9-11 DEG C in the photoperiod of L7-8/D16-17 for 11-14 days. The diapause aphidoletes aphidimyza pupae obtained by the induction method are stored in the absence of light at the temperature of 4-6 DEG C, the storage time is as long as 150 days, average eclosion rate after diapause relief is up to 75%, reproductive cost can be lowered remarkably by using the method for producing the aphidoletes aphidimyza, the diapause aphidoletes aphidimyza can be produced massively designedly in non-application season, after being stored to application season, the diapause aphidoletes aphidimyza is released from diapause to be applied to field, and product supply quantity of limited reproduction shops can be increased by more than three times.
Description
Technical field
The invention belongs to pest technical field of biological control, be specifically related to a kind ofly eat the method for aphid cecidomyiia Diapause inducement and the application in industrial production of syrphid eating cecidomyiia thereof.
Background technology
Food aphid cecidomyiia is the predator of the multiple aphid in greenhouse.Nowadays eaten aphid cecidomyiia by artificial propagation and be discharged into field and control aphid, be one of important measures changing the current governance model based on chemical pesticide control, tool is of great significance.
In food aphid cecidomyiia artificial propagation process, worm kind is preserved and is relied on anniversary successive propagation to realize always.Per in generation, goes through about 1 month phase, and the worm pupa low-temperature storage phase, more than one month, then worm pupa eclosion rate and Adult worms producting eggs amount significantly reduced generally within 1 month, therefore the anniversary must start Rearing facility, equipment, and special messenger keeps an eye on, causes the phenomenon that reproductive-cost is high.In addition, due to the anniversary, the food aphid cecidomyiia storage life of breeding is short, and the product only produced in small part period coincide with application period, and all the other are most all because depositing expired and abandoned, the phenomenon such as Product supply deficiency when causing a large amount of waste and apply.
Summary of the invention
The object of this invention is to provide a kind of method of eating aphid cecidomyiia Diapause inducement, the method can be used for producing food aphid cecidomyiia, and effectively can solve that worm pupa storage life of existing of successive propagation food aphid cecidomyiia anniversary in prior art is short, reproductive-cost is high, the problem such as undersupply, waste are serious.
In order to realize object of the present invention, inventor provide following technical scheme.
The method of food aphid cecidomyiia Diapause inducement, wherein, is placed in 9-11 DEG C in 20-26 DEG C/evening of daytime, 11-14 days under the condition of photoperiod L7-8/D16-17 by food aphid cecidomyiia ovum, namely obtains diapause state food aphid cecidomyiia pupa.
The method of above-mentioned food aphid cecidomyiia Diapause inducement, the condition of storage of described diapause state food aphid cecidomyiia pupa is 4-6 DEG C, lucifuge.
The method of food aphid cecidomyiia Diapause inducement is producing the application in food aphid cecidomyiia, comprising:
A. food aphid cecidomyiia ovum is placed in 9-11 DEG C in 20-26 DEG C/evening of daytime, 11-14 days under the condition of photoperiod L7-8/D16-17, obtains diapause state food aphid cecidomyiia pupa;
B. the diapause state food aphid cecidomyiia pupa lucifuge under 4-6 DEG C of condition obtained by step a stores;
C. the diapause state food aphid cecidomyiia pupa termination of diapause will stored in step b.
The method of above-mentioned food aphid cecidomyiia Diapause inducement is producing the application in food aphid cecidomyiia, and the time stored described in step b is 150 days.
The method of above-mentioned food aphid cecidomyiia Diapause inducement is producing the application in food aphid cecidomyiia, and termination of diapause described in step c adopts conventional method, and optimum condition is 25 ± 1 DEG C, photoperiod L14/D10.
In the method for food aphid cecidomyiia Diapause inducement of the present invention, inducing temperature condition be daytime 20-26 DEG C, evening 9-11 DEG C, as long as refer to that control inducing temperature induces and can normally carry out in described scope, do not need a certain temperature spot be constant at by inducing temperature in affiliated scope, the selected temperature range being suitable for inducing is very wide, effectively can reduce the cost needed for temperature control.
Daytime in described inducing temperature condition, evening are corresponding with L, D in the photoperiod respectively, and change is consistent.
Described condition of storage 4-6 DEG C refers in this temperature range and stores, and does not need a certain temperature spot be constant in this scope, effectively can reduce the cost needed for storage.
The method of food aphid cecidomyiia Diapause inducement of the present invention, utilize food aphid cecidomyiia certain sensitive worm state to accept principle that specified temp and photoperiod process enter diapause status, induce the diapause state food aphid cecidomyiia pupa obtained to store and reach 150 days, the average eclosion rate after termination of diapause is up to 75%.On the one hand, the cost of food aphid cecidomyiia breeding can significantly be reduced, on the other hand, can non-application in a planned way amount reproduction diapause state food aphid cecidomyiia in season, be stored to and apply season, be applied to field after termination of diapause, the Product supply amount more than 3 times in limited breeding workshop can be promoted.
Embodiment
Below in conjunction with specific embodiment, content of the present invention is further described in detail.
Embodiment
1, prepare food aphid cecidomyiia ovum: potted plant barley seedling, seedling inoculates corn leaf aphids, be placed in food aphid cecidomyiia output cage and lay eggs, obtain the potted plant wheat seeding of band aphid and food aphid cecidomyiia ovum.
2, Diapause inducement: process under the potted plant wheat seeding of band aphid and food aphid cecidomyiia ovum being placed in 20-26 on daytime DEG C/evening of alternating temperature 9-11 DEG C, photoperiod L7-8/D16-17 condition, aphid grows the predation of food aphid cecidomyiia egg hatching, larval phase is entered after 6-7 days, grew after 5-7 days for mature larva, water pond anastrophe is adopted to collect mature larva, be loaded in the plastic casing of band vermiculite, obtain diapause state food aphid cecidomyiia pupa.
3, long term storage: diapause state food aphid cecidomyiia pupa is put into 4-6 DEG C of refrigerator lucifuge and stores, 5 months storage lifves.
4, eclosion rate after storing: diapause state food aphid cecidomyiia pupa is after 4-6 DEG C of refrigerator stores 1,2,3,4,5,6 month respectively, and termination of diapause eclosion rate reaches 89.0%, 88.5%, 82.5%, 76.0%, 72.0%, 54.0% respectively.The condition of termination of diapause is temperature 25 ± 1 DEG C, photoperiod L14/D10.
Inventor is through repeatedly repetition test, and the average eclosion rate that the diapause state obtained according to the method for the invention food aphid cecidomyiia pupa stores 1,2,3,4,5,6 month is respectively 85%, 85%, 85%, 75%, 75%, 50%.
Claims (5)
1. eat the method for aphid cecidomyiia Diapause inducement, it is characterized in that, food aphid cecidomyiia ovum is placed in 9-11 DEG C in 20-26 DEG C/evening of daytime, 11-14 days under the condition of photoperiod L7-8/D16-17, namely obtain diapause state food aphid cecidomyiia pupa.
2. the method for food aphid cecidomyiia Diapause inducement according to claim 1, is characterized in that, the condition of storage of described diapause state food aphid cecidomyiia pupa is 4-6 DEG C, lucifuge.
3. the method for eating aphid cecidomyiia Diapause inducement is producing the application in food aphid cecidomyiia, it is characterized in that, comprising:
A. food aphid cecidomyiia ovum is placed in 9-11 DEG C in 20-26 DEG C/evening of daytime, 11-14 days under the condition of photoperiod L7-8/D16-17, obtains diapause state food aphid cecidomyiia pupa;
B. the diapause state food aphid cecidomyiia pupa lucifuge under 4-6 DEG C of condition obtained by step a stores;
C. the diapause state food aphid cecidomyiia pupa termination of diapause will stored in step b.
4. the method for food aphid cecidomyiia Diapause inducement according to claim 3 is producing the application in food aphid cecidomyiia, and it is characterized in that, the time stored described in step b is 150 days.
5. the method for food aphid cecidomyiia Diapause inducement according to claim 3 is producing the application in food aphid cecidomyiia, and it is characterized in that, the condition of termination of diapause described in step c is 25 ± 1 DEG C, photoperiod L14/D10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410586984.6A CN104304193A (en) | 2014-10-28 | 2014-10-28 | Aphidoletes aphidimyza diapause induction method and application thereof to production of aphidoletes aphidimyza |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410586984.6A CN104304193A (en) | 2014-10-28 | 2014-10-28 | Aphidoletes aphidimyza diapause induction method and application thereof to production of aphidoletes aphidimyza |
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| Publication Number | Publication Date |
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| CN104304193A true CN104304193A (en) | 2015-01-28 |
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| CN201410586984.6A Pending CN104304193A (en) | 2014-10-28 | 2014-10-28 | Aphidoletes aphidimyza diapause induction method and application thereof to production of aphidoletes aphidimyza |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371146A (en) * | 2018-02-12 | 2018-08-07 | 贵州大学 | A kind of release tech of food aphid cecidomyiia |
| CN110050762A (en) * | 2019-05-24 | 2019-07-26 | 河北省农林科学院旱作农业研究所 | A kind of method of hydroponics plant propagation aphid and food aphid cecidomyiia |
| CN110235860A (en) * | 2019-06-13 | 2019-09-17 | 中国农业科学院植物保护研究所 | The large-scale breeding method of Syrkhus corollae |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1830245A (en) * | 2006-03-28 | 2006-09-13 | 衡水田益生防有限责任公司 | Technology of industrial production of syrphid eating cecidomyiia |
| CN103155906A (en) * | 2013-04-02 | 2013-06-19 | 北京市农林科学院 | Aphidoletes aphidimyza mass reproduction technology and application technology |
| CN104041462A (en) * | 2014-07-03 | 2014-09-17 | 湖南农业大学 | Method for regulating and controlling diapause of microplitis syntretomorpha |
-
2014
- 2014-10-28 CN CN201410586984.6A patent/CN104304193A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1830245A (en) * | 2006-03-28 | 2006-09-13 | 衡水田益生防有限责任公司 | Technology of industrial production of syrphid eating cecidomyiia |
| CN103155906A (en) * | 2013-04-02 | 2013-06-19 | 北京市农林科学院 | Aphidoletes aphidimyza mass reproduction technology and application technology |
| CN104041462A (en) * | 2014-07-03 | 2014-09-17 | 湖南农业大学 | Method for regulating and controlling diapause of microplitis syntretomorpha |
Non-Patent Citations (4)
| Title |
|---|
| ANJA FORSBERG: "Possibilities of using the diapause of Aphidoletes aphidimyza (Rond.) (Diptera: Cecidomyiidae) in its mass production", 《SROP/WPRS BULLETIN》 * |
| SAAD ALOTAIBI: "Mass Production and Utilization of the Predatory Midge,Aphidoletes Rondani for Controlling Aphids", 《GLOBAL JOURNAL OF BIOTECHNOLOGY ＆ BIOCHEMISTRY》 * |
| 叶长青: "食蚜瘿蚊研究进展", 《昆虫知识》 * |
| 程洪坤等: "食蚜瘿蚊生物学初步研究", 《植物保护》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371146A (en) * | 2018-02-12 | 2018-08-07 | 贵州大学 | A kind of release tech of food aphid cecidomyiia |
| CN110050762A (en) * | 2019-05-24 | 2019-07-26 | 河北省农林科学院旱作农业研究所 | A kind of method of hydroponics plant propagation aphid and food aphid cecidomyiia |
| CN110235860A (en) * | 2019-06-13 | 2019-09-17 | 中国农业科学院植物保护研究所 | The large-scale breeding method of Syrkhus corollae |
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Application publication date: 20150128 |
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