CN103141443A - Method for regulating and controlling diapause of agryponflexorius - Google Patents
Method for regulating and controlling diapause of agryponflexorius Download PDFInfo
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Abstract
The invention discloses a method for regulating and controlling agryponflexorius, which adopts following steps: 1), agryponflexorius to be subjected to diapause induction is placed under the condition with the temperature of 17 plus/minus 1 DEG C and a photoperiod from (L8 : D16) to (L12 : D12) for induction, and is enabled to enter a diapause state; 2), the agryponflexorius entering the diapause state in the step 1 is stored under a dark condition with the temperature of 4 DEG C; and 3), after being ended in storage in step 2, the agryponflexorius resumes growth under a condition with temperature of 23 DEG C and a photoperiod from L14 to D10 to L16 to D8. The method has the advantages that the diapause induction rate is 100%, the storage period after diapause is 120 days, and after 20 days from diapause removal, adult agryponflexorius can be hatched. According to the invention, the agryponflexorius can be propagated in large, the shelf life of products and time in keeping prevention and control functions in fields can be prolonged, the prevention and treatment efficiency of insects is improved, and the method has important significance in knowing on developmental mechanism of natural enemy insects and exploring adaptive mechanism of insects to environment and evolutionary approaches of insects.
Description
Technical field
The invention belongs to biological technical field, relate to the method for a kind of beet webworm Ah lattice of regulation and control ichneumon wasp diapause.
Background technology
In Grassland ecosystems, biocoenosis exists with multiple biotic population form, and the relation with food chain between them links together, and wherein any ring changes, and will inevitably cause the variation of other links.Therefore in certain Grassland ecosystems scope, analyze the population of insect and the correlation of natural enemy population, strengthened artificially the strength of natural enemy population Control pests population, just can force down the level that can not work the mischief to herbage to pest population quantity.Utilizing the natural enemy insect pest control is methods most widely used, maximum in biological control.
Beet webworm Loxostege sticticalis Linnaeus(Lepidoptera: Pyralidae) be the sudden insects that migrate that eat of farming and animal husbandry more, damaging range is wide, feeding habits are assorted, larva can take food 300 various plants such as Chenopodiaceae, polygonaceae, pulse family, Solanaceae, Curcurbitaceae, Cruciferae, very large to China North China, northeast and the harm of the Northwest's farming and animal husbandry, and often break out and cause disaster, cause heavy economic losses.It is domestic that what for the control of beet webworm, mainly take is chemical control, spray appropriate organic synthesis insecticide, can play the effect that quick elimination reduces pest population density, but the excessive chemical agent of using, had a strong impact on the quality of crops, and cause environmental pollution and residue of pesticide, endanger human and livestock health.Therefore, change and currently take chemical control and be that main beet webworm prevents and treats the task of top priority that method has become Development of Forage Grass Industry, the pest controlling effect that takes full advantage of the abiogenous natural enemy insect in field is one of these important measures wherein, and tool is of great significance.
Beet webworm Ah lattice ichneumon wasp Agrypon flexorius Thunberg is subordinate to Hymenoptera, Ichneumonidae, Ah lattice's Uchida From China, it is the holometabola parasitic wasp, total ovum, larva, pupa and these four worm states (developmental stage) of adult in growth course, the development characteristics of each worm state is had nothing in common with each other and is not eaten motionless as ovum, pupa; Larva can only take food in pin main body, and mobility is very poor; Adult is had a wing, can circle in the air flexibly.Beet webworm Ah lattice ichneumon wasp is distributed in the areas such as Hebei, the Inner Mongol, Shanxi, is the migrate advantage parasitic wasp of Loxostege stieticatis larva of farming and animal husbandry.Investigation finds that beet webworm Ah lattice ichneumon wasp is beet webworm parasitized larvae at advanced age natural enemy, its population quantity and dominance index are all higher, population fluctuation trend is also similar with the population fluctuation trend of beet webworm, there is obvious following phenomenon, be the advantage honeybee of local parasitic beet webworm, the control beet webworm is broken out and plays an important role.In order to develop this valuable natural enemy resource, the approach that must adopt artificial propagation to discharge.But adopt tradition to connect the method for generation breeding, easily degenerating in the honeybee source, and is difficult for long preservation, to field, discharge and brought very large difficulty; By improving propagation technique, this honeybee of human-induced enters diapause status, can make storage life greatly extend, there is no the diapause control technique, often cause natural enemy insect before insect occurs with regard to dead, or insect while occurring the natural enemy insect product also do not produce, this is the bottleneck factor of serious restriction natural enemy insect industrialization production and large-area applications in the field of biological control at present exactly.Therefore utilize cryopreservation diapause worm cocoon technology, control the natural enemy development time, this is of crucial importance for expanding numerous natural enemy insect meaning the anniversary.
Summary of the invention
An object of the present invention is to provide the method for a kind of beet webworm Ah lattice of regulation and control ichneumon wasp diapause.
The method of regulation and control beet webworm Ah lattice ichneumon wasp provided by the present invention diapause, specifically can comprise the steps:
(1) the beet webworm Ah lattice ichneumon wasp of pending diapause being induced is placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and is induced, and makes described beet webworm Ah lattice ichneumon wasp enter the diapause state;
(2) the described beet webworm Ah lattice ichneumon wasp that enters diapause state step (1) obtained proceeds under 4 ℃ of temperature, dark condition and stores;
(3) after step (2) is stored and finished, described beet webworm Ah lattice ichneumon wasp is proceeded under the condition of 23 ℃ of temperature, photoperiod L14:D10 to L16:D8, recover to grow.
A further object of the present invention is to provide the diapause of a kind of beet webworm Ah lattice ichneumon wasp and induces and storage procedures.
Beet webworm Ah lattice ichneumon wasp provided by the present invention diapause is induced and storage procedures, specifically can comprise the steps:
(1) beet webworm Ah lattice ichneumon wasp is placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and is induced, make described beet webworm Ah lattice ichneumon wasp enter the diapause state;
(2) the described beet webworm Ah lattice ichneumon wasp that enters diapause state step (1) obtained proceeds under 4 ℃ of temperature, dark condition and stores.
An also purpose of the present invention is to provide a kind of beet webworm Ah lattice ichneumon wasp diapause abductive approach.
Beet webworm Ah lattice ichneumon wasp diapause abductive approach provided by the present invention, specifically can comprise the steps: beet webworm Ah lattice ichneumon wasp is placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and is induced, make described beet webworm Ah lattice ichneumon wasp enter the diapause state.
Three kinds of methods that provide for the invention described above, in one embodiment of the invention, in step (1), described temperature is specially 17 ℃; The described photoperiod is specially L8:D16, L10:D14 or L12:D12.
In one embodiment of the invention, in step (1), the intensity of illumination in described condition is 4000～5000LX; Relative air humidity in described condition is 70%-80%.
In three kinds of methods that provide in the invention described above, the life stages sensitive that the beet webworm Ah lattice ichneumon wasp that described pending diapause is induced experiences the diapause signal is ovum or 1 instar larvae.
When the beet webworm Ah lattice ichneumon wasp of inducing when described pending diapause specifically can be the ovum of beet webworm Ah lattice ichneumon wasp or 1 instar larvae, the time of inducing described in step (1) can be 2 days.
In one embodiment of the invention, in step (2), described relative air humidity is 60%-70%.
In first two method provided by the present invention, the time of storing described in step (2) can be in 120 days, was preferably in 80 days.
In one embodiment of the invention, in step (3), the intensity of illumination in described condition is 4000～5000Lx; Relative air humidity in described condition is 70%-80%.
In one embodiment of the invention, the time that described in step (3), recovery is grown specifically can be 20 days.
The method of regulation and control provided by the present invention beet webworm Ah lattice ichneumon wasp diapause, the diapause inductivity is nearly 100%, and can reach 120 days the storage period after diapause, and diapause is removed and within latter 20 days, can be obtained the one-tenth honeybee sprouted wings.The present invention not only contributes to promote in production practices the vast propagation of beet webworm Ah lattice ichneumon wasp, extends its product shelf phase and, in the prevention and control action time in field, also contributes to grasp the development characteristics of natural enemy insect, improves control of insect efficiency.
The accompanying drawing explanation
Fig. 1 is the diapause impact on beet webworm Ah lattice ichneumon wasp of temperature and photoperiod.
Fig. 2 is the sensitive period analysis result that beet webworm Ah lattice ichneumon wasp experiences the diapause signal.
Fig. 3 is the impacts of different cold preservation times on beet webworm Ah lattice ichneumon wasp diapause cocoon.
Embodiment
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
For trying the worm source:
Beet webworm: be recorded in " toxicity test of different beauveria bassianas to beet webworm, " Chinese plant protection guide " the 10th phases in 2011 such as Wu Jinhua, Liu Aiping " literary composition, the public can obtain from grassland research institute, China Agriculture academy of sciences.Beet webworm is as supplying the examination host, (23 ℃ of climatic cabinates in laboratory, L16:8D, light intensity 8800Lx) in, with the pea seedlings subculture, raise, when being used as host's food, pea seedlings chooses the potted plant of growing to 5-6 sheet leaf, the clip cauline leaf is raised the beet webworm larva, feed 1-2 every day, in order to reduce the death of larva, should reduce the brush to larva while changing blade hinders or stirs as far as possible, adopt shake gently or concussion blade to make the larva self falling to fresh blade, 1 age～2 instar larvae ambient humidity of living in remains on 70%-80%, 3-5 instar larvae environment of living in remains on 60% left and right.
Beet webworm Ah lattice ichneumon wasp: be recorded in " beet webworm parasitic wasp and to the control action of host population, Tian Xiaoxia, Chinese Academy of Agricultural Sciences's Master's thesis, 2010 " literary composition, the public can obtain from grassland research institute, China Agriculture academy of sciences.(23 ℃ of climatic cabinates, RH60%, L16:8D, light intensity 8800Lx) interior with beet webworm 3-5 instar larvae even generation raising beet webworm Ah lattice ichneumon wasp, the honeybee worm is than being 1:40, let alone abundant ovipositing hosts, observe day by day, after the beet webworm larva buries and spins, spray suitable quantity of water every day, keep the certain humidity of soil, be placed under old terms and treat its emergence, the beet webworm Ah lattice ichneumon wasp of getting in emergence 24h waits to try.
Test apparatus and material
The impact test that temperature and photoperiod induce the diapause of beet webworm Ah lattice ichneumon wasp is all in climatic cabinate (SPX-300I-G type, the rich Medical Equipment Plant of industry Co., Ltd that proves to be true after interrogation in Shanghai) in, carry out, climatic cabinate temperature step-up error is ± 1 ℃, and the relative moisture step-up error is ± 5%.The raising of beet webworm larva and beet webworm Ah lattice ichneumon wasp is all carried out in the self-control dependent insect cage, connects for population and raises in large dependent insect cage (45cm * 45cm * 30cm), and in test, little dependent insect cage specification used is 30cm * 20cm * 20cm(length * wide * height).Separately need flat based tubes (6cm * 1.5cm), mercurial thermometer, writing brush, absorbent cotton, elastic tape, pallet, culture dish, preservative film, vermiculite etc. are some.
The experimental technique used in following embodiment if no special instructions, is conventional method.
When following embodiment implementation data is analyzed, all utilize DPS to carry out variance analysis, significance test of difference adopts the Duncans duncan's new multiple range method.
In following embodiment, photoperiod Lx:Dy means light irradiation time x hour every day, dark duration y hour, x+y=24.
Following embodiment all adopts international parasitic wasp diapause criterion, and basic principle is based on the rule of effective temperature summation, when certain worm state of certain parasitic wasp remains unfulfilled growth in the twice that should complete development duration in the time, assert it is to enter diapause status.More concrete, judge in the present invention whether beet webworm Ah lattice ichneumon wasp enters diapause status and determine as follows: if this worm state of beet webworm Ah lattice ichneumon wasp " pupa " remains unfulfilled growth within the twice time that should complete development duration (30 days), from larva buries while spinning, in 30 days, beet webworm Ah lattice ichneumon wasp fails to sprout wings, and assert that beet webworm Ah lattice ichneumon wasp enters diapause status.
The diapause of embodiment 1, beet webworm Ah lattice ichneumon wasp is induced
One, beet webworm Ah lattice ichneumon wasp diapause temperature and photoperiodic definite
Adopt two-factor orthogonal experiment, 5 different photoperiod (L8:D16 are set, L10:D14, L12:D12, L14:D10, L16:D8) (17 ℃ of and 4 different temperatures, 20 ℃, 23 ℃, 26 ℃), totally 20 processing, each processes 30 beet webworm larvas (host), repeat 3 times, beet webworm Ah lattice ichneumon wasp queen bee after mating 1d is parasitized to the beet webworm larva, then proceed in the insect box that sandy soil are housed, put into the climatic cabinate of different photoperiods and Temperature Treatment (wherein, intensity of illumination during illumination is 4000～5000LX, relative air humidity is 70%-80%), beet webworm Ah lattice ichneumon wasp is carried out to diapause and induce (diapause is induced and is considered as from " ovum " this worm state), induction time is 2 days, be transferred to the normal raising condition of beet webworm Ah lattice ichneumon wasp after inducing, be (23 ℃ of climatic cabinates, RH60%, L16:8D, light intensity 8800Lx).In experiment, provide fresh pea seedlings for the beet webworm larva every day.The growth of observed and recorded beet webworm larva (host) and beet webworm Ah lattice ichneumon wasp queen bee (parasitic wasp) developmental state, formation and temperature and the relation between the photoperiod of statistics parasitic wasp Diapause.
Result, as shown in table 1 and Fig. 1, is under 17～26 ℃ of conditions in temperature, and in light application time 8～16h scope, along with the reduction of temperature and the shortening of light application time, the diapause rate obviously increases.Temperature or photoperiod beet webworm Ah lattice ichneumon wasp diapause rate all to be had to significant impact.As can be seen from Table 1, when temperature is 26 ℃, light application time 8～16h, do not have the diapause phenomenon to occur; Temperature is 23 ℃, light application time 12～8h, the individual diapause (0.7%～13.6%) of minority; When temperature is 20 ℃, the diapause rate obviously improves, and the diapause rate of light application time 16～8h is respectively 2.8%, 8.6%, 61.6%, 70.3% and 90.5%; When mean temperature is 17 ℃, light application time is lower than 12h, and 100% individuality enters diapause.As can be seen from Figure 1, low temperature, short illumination are the principal elements of inducing the diapause of beet webworm Ah lattice ichneumon wasp, and temperature plays a leading role, and the photoperiod only works in certain temperature range; In the time of 26 ℃, short illumination can not be induced diapause.
Temperature and the photoperiod impact on the diapause of beet webworm Ah lattice ichneumon wasp under table 1 indoor conditions
Annotate: in table, data are mean value ± standard errors, different lowercase alphabet differentials different significantly (p<0.05) after data, and different capitalizations mean extremely significantly (p<0.01) (Duncan ' s duncan's new multiple range method) of difference.
Two, beet webworm Ah lattice ichneumon wasp diapause regulation and control life stages sensitive determines
Induce result according to the light temperature diapause in step 1, in 17 ℃ of temperature, the short illumination of photoperiod L8: D16() the diapause environment, and the long illumination of 23 ℃ of temperature, photoperiod L16: D8() non-diapause environment, intensity of illumination is 4000～5000LX, relative air humidity is under the condition of 70%-80%, tests as follows:
A. will be put into above-mentioned non-diapause environment by the beet webworm larva (host) of beet webworm Ah lattice ichneumon wasp parasitism on the same day, process 0,2,4,6,9, after 13d, transfer in above-mentioned diapause environment 2 days.40 host larvas are established in each processing, repeat 3 times, record the quantity of non-diapause cocoon and diapause cocoon.
B. will by the beet webworm larva (host) of beet webworm Ah lattice ichneumon wasp parasitism, after the processing respectively 3,6,9,12,16 in above-mentioned diapause environment, 18d, be transferred in above-mentioned non-diapause environment on the same day.Each processes 40 host larvas, repeats 3 times, records the quantity of non-diapause cocoon and diapause cocoon.
Result, as shown in table 2 and Fig. 2, if at first grown 2 days in non-diapause environment by parasitic host larva, is then transferred in the diapause environment, and the diapause rate of cocoon is 100%, and now beet webworm Ah lattice ichneumon wasp is 1 instar larvae; Grow and within 6 days, transfer in the diapause environment, the diapause rate of cocoon drops to 4.5%, grows more than 9 days and moves on in the diapause environment again, does not have diapause to occur.If will at first be grown 6 days in the diapause environment by parasitic host larva, to transfer in non-diapause environment, the diapause rate of cocoon is 0; Grow and within 9 days, transfer under non-diapause condition again, the diapause rate is 5.9%, and now beet webworm is 3 instar larvaes; Grow and transfer to again more than 12 days under non-diapause condition, diapause rate 100%.Thus, the life stages sensitive that beet webworm Ah lattice ichneumon wasp experiences the diapause signal is ovum and 1 instar larvae, after ovum and 1 instar larvae are experienced the diapause signal, need under the diapause condition, grow to mature larva and could all enter diapause.
Table 2 beet webworm Ah lattice ichneumon wasp experiences the sensitive period of diapause signal
Annotate: in table, data are mean value ± standard errors, different lowercase alphabet differentials different significantly (p<0.05) after data, and different capitalizations mean extremely significantly (p<0.01) (Duncan ' s duncan's new multiple range method) of difference.Wherein, " developmental stage " is by being dissected rear discovery to polypide: from host's 3 instar larvaes, start to dissect, dissect 5 larvas every 2d, treat that larva buries and continue to dissect, observe the worm state of pin main body endoparasitism honeybee, observe the also definite development time of each developmental condition in length of time of parasitoid larva under stereomicroscope, result is as follows: in 23 ℃ of not diapause situations, Ah lattice ichneumon wasp is by egg development to prepupa experience about 13 days, and the development time of 1 age, 2 ages, 3 ages, 4 ages, 5 instar larvaes is respectively 2d, 2d, 3d, 2d, 2d; In 17 ℃ of diapause situations, by egg development, to prepupa experience about 18 days, the development time of 1 age, 2 ages, 3 ages, 4 ages, 5 instar larvaes was respectively 3d, 3d, 3d, 4d, 2d.
The result of comprehensive embodiment 1, as seen for beet webworm Ah lattice ichneumon wasp ovum or 1 instar larvae, the envirment factor that imposes 17 ℃ of temperature, photoperiod L8:D16 to L12:D12 stimulates 2 days, can induce 100% individuality to enter the diapause state.
The diapause maintenance of embodiment 2, beet webworm Ah lattice ichneumon wasp
The present embodiment is by measuring the eclosion rate of beet webworm Ah lattice ichneumon wasp diapause cocoon, and life-span, egg-laying period and the parasitic rate of the one-tenth honeybee after sprouting wings, determines the time of low-temperature storage beet webworm Ah lattice ichneumon wasp diapause cocoon.Specific as follows:
1, the eclosion rate of beet webworm Ah lattice ichneumon wasp diapause cocoon
The beet webworm Ah lattice ichneumon wasp (now outward appearance is the worm cocoon) of the diapause state that the method that adopts embodiment 1 is obtained is collected in moistening soft soil, divide and install to (diameter 10cm in plastic bowl, high 8cm), plastic bowl covers with ventilative gauze, be stored in the refrigerating box under full dark, 4 ℃, a water of spray in every 3 days, maintain relative air humidity 60%-70%.Regularly the diapause cocoon is taken out and puts into 23 ℃ of temperature, photoperiod L14 from refrigerator: the D10 incubator carries out catalysis, observes and add up the eclosion rate of parasitic wasp diapause cocoon after the refrigeration different time.8 processing (8 cold preservation times, in Table 3) are established in experiment altogether, and each processes 40～60 diapause cocoons, repeats 3 times.
2, the beet webworm Ah lattice ichneumon wasp after emergence becomes life-span, egg-laying period and the parasitic rate of honeybee
One-tenth honeybee after the diapause cocoon that step 1 is obtained is sprouted wings is put into insect box, every box is introduced a pair of beet webworm Ah lattice ichneumon wasp (1 queen bee+1 drone), hydromel nutrition as a supplement with mass fraction 10%, mating 24h under the condition of 23 ℃ of temperature, photoperiod L14: D10, then inoculated, soon the beet webworm Ah lattice ichneumon wasp queen bee after mating 1d parasitizes the beet webworm larva.Each processes 4～5 pairs of parasitic wasps of inoculation, repeats 3 times.50 of every pair of honeybee inoculations beet webworm larva in 4 age, for the queen bee parasitism, is taken out female drone and continues inoculation, until female drone death after 24h.The non-diapause cocoon that does not refrigerate processing of take is contrast (CK).Life-span, egg-laying period and the parasitic rate of one-tenth honeybee after statistics is sprouted wings.Wherein, the egg-laying period is the time interval of observing between laying eggs for the first time and laying eggs for the last time, in practical operation, usually by sky, calculates.Parasitic rate is divided by total beet webworm number by parasitic beet webworm number.
3, result
Result is as shown in table 3 and Fig. 3, the beet webworm Ah lattice ichneumon wasp diapause cocoon that the method that adopts embodiment 1 is obtained is transferred to 23 ℃ of catalysis about 4 ℃ refrigerations after 80 days, its eclosion rate is 80.5%, the male approximately 13 days peak life-span, approximately 14 days queen bee life-span, lay eggs 4.4 days duration, parasitic rate reaches 30.8%, not remarkable with control group difference.Diapause cocoon refrigeration 120 days, although the indexs such as female drone life-span, egg-laying period are starkly lower than control group, but still have 71.7% diapause cocoon normally to sprout wings, and in approximately 3 days egg-laying period, parasitic rate is 20.4%.
The impact of table 3 refrigeration on the diapause cocoon
Annotate: in table, data are mean value ± standard errors, different lowercase alphabet differentials different significantly (p<0.05) after data, and different capitalizations mean extremely significantly (p<0.01) (Duncan ' s duncan's new multiple range method) of difference.
The result of cumulated volume embodiment, visible for the beet webworm Ah lattice ichneumon wasp (diapause cocoon) that enters the diapause state, be stored under full dark, 4 ℃ of conditions, can reach 120 days storage period.
The diapause of embodiment 3, beet webworm Ah lattice ichneumon wasp is removed
The diapause state beet webworm Ah lattice ichneumon wasps (diapause cocoon) that obtain for the method that adopts embodiment 1, can be by thermal stimulus, and the termination of diapause state, enter development process, and concrete measure is as follows:
Beet webworm Ah lattice ichneumon wasp diapause cocoon is placed in to the environment of 23 ℃ of temperature, photoperiod L14:D10 to L16:D8, luminous intensity 4000-5000Lx, a water of spray in every 3 days, keep certain humidity (RH is 70%-80%), get final product the termination of diapause state after 20 days, the diapause cocoon all turns into into honeybee very soon.
Wherein, induction time is determined in 20 days as follows:
Beet webworm Ah lattice ichneumon wasp diapause cocoon after the method that adopts embodiment 2 is stored to 120 days under full dark, 4 ℃ of conditions proceeds in the incubator (RH is 70%-80%) of 23 ℃ of temperature, photoperiod L14: D10 and carries out the diapause releasing, the emergence time of statistics parasitic wasp diapause cocoon, adopt 60 diapause cocoons to be tested, establish altogether repeated experiments 3 times.Result shows, from the diapause cocoon being proceeded under 23 ℃ of conditions when starting to carry out diapause and removing, approximately after 20 days, the diapause cocoon all turns into into honeybee, termination of diapause state very soon.Three repeated experiments are indifference as a result.
Claims (8)
1. a method that regulates and controls the diapause of beet webworm Ah lattice ichneumon wasp, comprise the steps:
(1) the beet webworm Ah lattice ichneumon wasp of pending diapause being induced is placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and is induced, and makes described beet webworm Ah lattice ichneumon wasp enter the diapause state;
(2) the described beet webworm Ah lattice ichneumon wasp that enters diapause state step (1) obtained proceeds under 4 ℃ of temperature, dark condition and stores;
(3) after step (2) is stored and finished, described beet webworm Ah lattice ichneumon wasp is proceeded under the condition of 23 ℃ of temperature, photoperiod L14:D10 to L16:D8, recover to grow.
2. beet webworm Ah lattice ichneumon wasp diapause is induced and storage procedures, comprises the steps:
(1) beet webworm Ah lattice ichneumon wasp is placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and is induced, make described beet webworm Ah lattice ichneumon wasp enter the diapause state;
(2) the described beet webworm Ah lattice ichneumon wasp that enters diapause state step (1) obtained proceeds under 4 ℃ of temperature, dark condition and stores.
3. a beet webworm Ah lattice ichneumon wasp diapause abductive approach, comprise the steps: beet webworm Ah lattice ichneumon wasp is placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and is induced, and makes described beet webworm Ah lattice ichneumon wasp enter the diapause state.
4. according to arbitrary described method in claim 1-3, it is characterized in that: ovum or 1 instar larvae that the beet webworm Ah lattice ichneumon wasp that described pending diapause is induced is beet webworm Ah lattice ichneumon wasp.
5. method according to claim 4, it is characterized in that: the time of inducing described in step (1) is 2 days.
6. according to arbitrary described method in claim 1-5, it is characterized in that: the time of storing described in step (2) is in 120 days.
7. method according to claim 6, it is characterized in that: the time of described storage is in 80 days.
8. according to arbitrary described method in claim 1-7, it is characterized in that: the time of recovering described in step (3) to grow is 20 days.
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| CN108633844A (en) * | 2018-04-26 | 2018-10-12 | 贵州大学 | A method of assessing Karst ecosystem revegetation potentiality using soil animal |
| CN109874752A (en) * | 2019-04-03 | 2019-06-14 | 中国农业科学院植物保护研究所 | A kind of diapause regulation of the endoparasitoid Microcentrus cingulum and storage method |
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| CN108633844B (en) * | 2018-04-26 | 2021-03-02 | 贵州大学 | Method for evaluating recovery potential of karst ecosystem by utilizing soil animals |
| CN112219798A (en) * | 2018-05-30 | 2021-01-15 | 吉林省林业科学研究院 | Method for cultivating Chouioia cunea Yang |
| CN112219798B (en) * | 2018-05-30 | 2022-01-11 | 吉林省林业科学研究院 | Method for cultivating Chouioia cunea Yang |
| CN109874752A (en) * | 2019-04-03 | 2019-06-14 | 中国农业科学院植物保护研究所 | A kind of diapause regulation of the endoparasitoid Microcentrus cingulum and storage method |
| CN109874752B (en) * | 2019-04-03 | 2021-04-09 | 中国农业科学院植物保护研究所 | Diapause regulation and storage method for waistband long body hornets |
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