CN103704178B - A kind of regulate and control full skirt chase after the method for posting fly diapause - Google Patents
A kind of regulate and control full skirt chase after the method for posting fly diapause Download PDFInfo
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Abstract
The invention discloses a kind of regulate and control full skirt chase after the method for posting fly diapause.The method comprises the steps: 1) full skirt of pending Diapause inducement is chased after post under fly is placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12 and induce, described full skirt is chased after post fly to enter diapause state; 2) the described full skirt entering diapause state step 1) obtained chases after to post under fly proceeds to temperature 4 DEG C, dark condition and stores; 3) step 2) after storage terminates, described full skirt is chased after and posts under fly proceeds to the condition of temperature 23 ± 1 DEG C, photoperiod L14:D10 to L16:D8, recover to grow.The method Diapause inducement rate 100%, the storage period after diapause can reach 120 days, within after Diapause termination 20 days, can obtain the one-tenth fly sprouted wings.The present invention chases after the vast propagation posting fly to full skirt, extend its product shelf phase and the prevention and control action time in field, improve control of insect efficiency, deepen the understanding to natural enemy insect developmental mechanism, explore insect all significant to the adaptation mechanism of environment and evolutionary path etc.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of regulate and control full skirt chase after the method for posting fly diapause.
Background technology
Natural enemy insect is a class parasitism or the insect preying on other insects, and they control the development of insect and spread in farmland, forest zone and pasture.China's natural enemy insect resource is very abundant, has the material base of strong Application and Development.By development natural enemy insect large-scale production technology; adopt the technology of indoor amount reproduction natural enemy insect method and a large amount of release; the population quantity of the initial natural enemy in field can be increased; in conjunction with biopesticide and ecological technique; form comparatively complete techniques of pest control system; substitute or reduce access times and the consumption of chemical pesticide, protection of the environment and bio-diversity, guarantee agricultural product production safety.Therefore, study natural enemy insect artificial feeding scale, merchandized handling etc. and there is important application and promotional value.Carry out the dark people research of Insect Diapause, can be the mankind and can understand insect and grow and the rule of growth and decline, finally for Unpolluting management insect, utilize beneficial insect to provide scientific theory foundation.
Beet webworm LoxostegesticticalisL. belongs to Lepidoptera, Pyralidae class insect, is a kind of universal farming and animal husbandry insect, because its host plant is of a great variety, is that a kind of intermittence is broken out and has destructive migratory pest.In beet webworm parasitic insect, the parasitic rate of parasitic fly to beet webworm is higher than parasitic wasp, plays important regulating and controlling effect to Host biotypes.The abundantest with parasitic fly in the natural enemy monoid of beet webworm, be the key factor controlling beet webworm occurrence injury and population dynamic.
Full skirt chases after to be posted fly (ExoristacivilisRond.) and belongs to diptera Larvaevoridae (Diptera:Tachinidae), it is holometabola parasitic fly, total ovum, larva, pupa and these four worm states (developmental stage) of adult in growth course, the development characteristics of each worm state is had nothing in common with each other, as ovum, pupa are not eaten motionless; Low instar larvae can only take food in pin main body, and mature larva then climbs out of in worm pin main body, namely pupates in 12h---24h, enters and does not eat motionless state.Adult then has a flight performance wing, can circle in the air flexibly.
One of considerable advantage parasite on full skirt Zhui Jiyingshi China beet webworm larva.Although overcome the drawback that Traditional Man subculture is bred, full skirt can be made to chase after post fly can carry out annual accumulation and breed.But adopt tradition to connect the method for generation breeding, fly easily degenerates, and not easily preserves for a long time, brings very large difficulty to field adaptability.By improving propagation technique, this fly of human-induced enters diapause status, and storage life can be made greatly to extend, and therefore utilizes cryopreservation diapause worm cocoon technology, and control natural enemy development time, this is of crucial importance for expanding numerous natural enemy insect meaning the anniversary.By hold each generation process of breeding and accurately target of prediction insect time of origin and occur quantity, when insect occurs, natural enemy insect can be discharged timely, thus reach the effect of control of insect.
Summary of the invention
The object of this invention is to provide a kind of regulate and control full skirt chase after the method for posting fly diapause.
Regulation and control full skirt provided by the present invention chases after the method for posting fly diapause, specifically comprises the steps:
(1) full skirt of pending Diapause inducement is chased after post under fly is placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12 and induce, described full skirt is chased after post fly to enter diapause state;
(2) the described full skirt entering diapause state that step (1) obtains is chased after post under fly proceeds to temperature 4 DEG C, dark condition and store;
(3), after step (2) storage terminates, described full skirt is chased after and posts under fly proceeds to the condition of temperature 23 ± 1 DEG C, photoperiod L14:D10 to L16:D8, recover to grow.
Another object of the present invention is to provide a kind of full skirt and chases after and post fly Diapause inducement and storage procedures.
Full skirt provided by the present invention chases after posts fly Diapause inducement and storage procedures, specifically can comprise the steps:
(1) full skirt is chased after post under fly is placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12 and induce, described full skirt is chased after post fly to enter diapause state;
(2) the described full skirt entering diapause state that step (1) obtains is chased after post under fly proceeds to temperature 4 DEG C, dark condition and store.
Another object of the present invention is to provide a kind of full skirt and chases after and post fly Diapause inducement method.
Full skirt provided by the present invention chases after posts fly Diapause inducement method, full skirt specifically can be comprised the steps: to chase after post under fly is placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12 to induce, and described full skirt is chased after post fly to enter diapause state.
For three kinds of methods that the invention described above provides, in the present invention, in step (1), described temperature is specially 16 DEG C, 17 DEG C or 18 DEG C; The described photoperiod is specially L8:D16, L10:D14 or L12:D12.
For three kinds of methods that the invention described above provides, in step (1), the intensity of illumination in described condition is 4000-5000LX; Relative air humidity in described condition is 60%-70%.
For three kinds of methods that the invention described above provides, in step (1), the full skirt of described pending Diapause inducement chases after and posts fly is that full skirt chases after the ovum or larva of posting fly; Described full skirt is chased after and posts time that fly carries out Diapause inducement and at least contain described full skirt and chase after the larval phase of posting fly.
Concrete, in the present invention, in step (1), described " chased after by the full skirt of pending Diapause inducement to post under fly is placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12 and induce " is: chased after by the described full skirt of ovum phase to post under fly is placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12 and induce, and to be chased after by described full skirt post fly and take out from the condition of described temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12 in pupa time or adult stage.
In the step (2) of said method, within time of described storage can be 120 days, within being preferably 60 days, as 30-60 days.Relative air humidity in described condition is 60%-70%, as 70%.
In the step (3) of said method, the described time of recovering to grow can be 20 days, and after 20 days, described full skirt chases after and posts fly and can turn into adult.Intensity of illumination in described condition is 5000LX; Relative air humidity in described condition is 60%-70%, as 70%.
In the present invention, chasing after to described full skirt the host posted when fly carries out Diapause inducement, diapause maintenance or Diapause termination is beet webworm, as the beet webworm at the beginning of 4 age Mo ~ 5 ages.
Regulation and control full skirt provided by the present invention chases after the method for posting fly diapause, Diapause inducement rate nearly 100%, and the storage period after diapause can reach 120 days, and after Diapause termination, 20 day after tomorrow can obtain the one-tenth fly sprouted wings.The present invention not only contributes to promoting full skirt to chase after in production practices and posts the vast propagation of fly, extends its product shelf phase and the prevention and control action time in field, also contributes to the development characteristics grasping natural enemy insect, improves control of insect efficiency.
Accompanying drawing explanation
Fig. 1 chases after the impact of posting fly diapause in the temperature and light cycle on full skirt.
Fig. 2 be 16 DEG C and 18 DEG C time full skirt chase after the critical photoperiod of the Diapause inducement posting fly.
Embodiment
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
For examination worm source:
Full skirt chases after posts fly: be recorded in " Xiang Hongyan; Liu Aiping; Gao Shujing; Xu Linbo; Liu Changzhong. beet webworm advantage parasite---full skirt chases after posts fly biological characteristic research [J]. Acta Prataculture; 2013,22 (3): 92-98 " in a literary composition, the public can obtain from grassland research institute, China Agriculture academy of sciences.Full skirt chases after to be posted fly adult and raises in the disposable transparent water tumbler of 240ml, and rim of a cup gauze covers, and gauze is sewed with rayon balls, and uses 15%(volume fraction) hydromel soaks.
Beet webworm: be recorded in " Wu Jinhua, Liu Aiping etc. artificial feed are on the impact [J] of beet webworm digestive enzyme activity and carboxy-lesterase mrna expression amount. meadow journal; 2012; 20(6):: 169-1174 " in a literary composition, the public can obtain from grassland research institute, China Agriculture academy of sciences.Beet webworm ovum being placed in the radius being equipped with filter paper is the glass culture dish of 15cm, and wherein filter paper should keep moistening.After beet webworm hatching, to feed beet webworm with fresh goosefoot leaf or cloverleaf, until when beet webworm larva was 3 age, turned by larva and raise in the plastic casing of length × wide=15cm × 11cm, residing for 3-5 instar larvae, environment remains on about 60%.Every day feeds 1-2 time, and changes feed in time and screen out worm excrement.In order to reduce the death of larva, when should reduce replacing blade as far as possible, the brush of larva being hindered or being stirred, adopting shake gently or concussion blade to make larva self falling on fresh blade.
Test apparatus and material
The temperature and light cycle chases after the impact of posting fly Diapause inducement to full skirt and tests all in climatic cabinate (SPX-300I-G type, Shanghai Medical Equipment Plant of Bo Xun Industrial Co., Ltd.) in carry out, climatic cabinate temperature step-up error is ± 1 DEG C, and relative moisture step-up error is ± 5%.Beet webworm larva and full skirt chase after the raising of posting fly all to carry out in self-control dependent insect cage.Separately need flat based tubes (6cm × 1.5cm), mercurial thermometer, writing brush, absorbent cotton, elastic tape, pallet, culture dish, preservative film, vermiculite etc. are some.
The experimental technique used in following embodiment if no special instructions, is conventional method.
When following embodiment implementation data is analyzed, all utilize DPS to carry out variance analysis, significance test of difference adopts Duncans duncan's new multiple range method.
In following embodiment, photoperiod Lx:Dy represents light irradiation time x hour every day, dark duration y hour, x+y=24.Photoperiod LL represents light irradiation time 24 hours every day, dark duration 0 hour; Photoperiod DD represents light irradiation time 0 hour every day, dark duration 24 hours.
Following embodiment all adopts international parasitic fly diapause criterion, and general principle is based on the rule of effective temperature summation, when certain worm state of certain parasitic fly remains unfulfilled growth within three times of times of the shortest development duration, namely assert it is enter diapause status.More concrete, judge in the present invention that full skirt chases after to post fly and whether enter diapause status and determine as follows: if full skirt chases after and posts this worm state of fly " larva " remain unfulfilled growth within three times of times (namely 27 days) of the shortest development duration, namely from egg hatching becomes larva, in 27 days, full skirt chases after the larva of posting fly and fails to enter " pupa " this worm state, then assert that full skirt chases after and post fly and enter diapause status.
Embodiment 1, full skirt chase after the Diapause inducement posting fly
One, full skirt chases after the determination of posting the fly diapause temperature and light cycle
Arrange 4 different temperatures (14 DEG C, 17 DEG C, 21 DEG C and 25 DEG C) photoperiod different from 5 (L8:D16, L10:D14, L12:D12, L14:D10, L16:D8), totally 20 process, healthy and strong beet webworm larva (host) at the beginning of each process 30 4 age Mo ~ 5 ages, repeats 3 times.Full skirt after mating 1d is chased after and posts the female fly of fly and beet webworm larva is together placed in insect box, put into the climatic cabinate of different photoperiod and Temperature Treatment (wherein, intensity of illumination during illumination is 4000 ~ 5000LX, relative air humidity is 60%-70%), full skirt is chased after and posts fly and carry out Diapause inducement (Diapause inducement from " ovum " this worm state), induction 10-12 days.After having induced, proceed to normal rearing conditions, namely 23 DEG C, L16:D8, relative air humidity be raise in the illumination box of 60%, each processed group full skirt of observed and recorded chases after the diapause situation of posting fly, and polypide developmental state.
Result is as shown in table 1 and Fig. 1, and photoperiod and temperature are that full skirt chases after the Major environment impacts factor of posting fly Diapause inducement.To be temperature or photoperiod on full skirt chase after posts fly Diapause rate and all has significant impact.When 17 DEG C, Diapause rate is the highest, and namely 17 DEG C is best diapause temperature.Lower than 17 DEG C, larva activity is slow, stiff.Death toll increases.21 DEG C time, along with the increase of light application time, Diapause rate declines gradually; 25 DEG C time, diapause can not be induced.Visible, low temperature, short photoperiod are that induction full skirt chases after the principal element of posting fly diapause, and temperature plays a leading role, and the photoperiod only works in certain temperature range.
Under table 1 indoor conditions, the temperature and light cycle chases after the impact of posting fly diapause on full skirt
Note: in table, data (mean value ± standard error) newly answer range test through DuncanShi, after each row of data, different capitalization represents that difference is extremely significantly (P < 0.01); Different lowercase alphabet shows significant difference (P < 0.05)." cocoon " in table in " cocoon number " for beet webworm pupate after the cocoon tied, namely investigate cocoon number, because full skirt chases after, to post fly be grow in pin main body, finally sprouts wings into adult, thus observed be beet webworm cocoon.
Two, full skirt chases after the determination of posting fly Diapause inducement critical photoperiod
Under 16 DEG C and 18 DEG C of conditions, establish the photoperiod process that LL, L2:D22, L4:D20, L6:D18, L8:D16, L10:D14, L12:D12, L14:D10, L16:D8, L18:D6, L20:D4, L22:D2, DD13 different respectively, amount to 26 process.
Select the healthy and strong beet webworm larva at the beginning of 4 age Mo ~ 5 ages, put into connect fly case (40cm × 30cm × 25cm) bottom fresh alfalfa leaf or goosefoot leaf on, full skirt after mating 1d being chased after and posts the female fly of fly and put into and connect fly case, will 15%(volume fraction be soaked with) rayon balls of the hydromel end face that hangs over fly case chases after the food source of posting fly adult as full skirt.Remove full skirt after 24h to chase after and post fly, beet webworm is proceeded in vial respectively and continues to raise with fresh alfalfa leaf or goosefoot leaf, 50 the every bottle incubators being placed in different disposal respectively.Illumination box ambient As temperature error ± 1 DEG C, intensity of illumination 4000 ~ 5000LX, relative air humidity 60%-70%.Each process repetition 3 times.Observe full skirt under recording the different photoperiod and chase after the diapause situation of posting fly, thus determine that full skirt chases after and post fly Diapause inducement critical photoperiod.
As shown in Figure 2, can find out, light application time is greater than 16h(L>16h to result) time, the full skirt of 100% chases after to be posted fly individuality and all can grow, and does not occur diapause situation.The ratio of high diapause only appears between short-day 8 ~ 12h; At the short-day of below 8h, the ratio of diapause obviously declines with the shortening at sunshine, complete every day 2h sunshine and dark under, whole ontogeny.Critical day-length is 12.53h 16 DEG C time as seen from the figure, is 11.12h 18 DEG C time.
Three, full skirt chases after the determination of posting fly diapause regulation and control life stages sensitive
According to the result of the light temperature Diapause inducement in step one and step 2, by temperature 16 DEG C, photoperiod L10: D14(short photoperiod, S) as induction diapause condition, by temperature 16 DEG C, photoperiod L16: D8(long illumination, L) as non-diapause condition, following life stages sensitive determination test is carried out.Intensity of illumination in process of the test is 5000LX, and relative air humidity is 60%.
Select the healthy and strong beet webworm larva at the beginning of 4 age Mo ~ 5 ages, put into connect fly case (40cm × 30cm × 25cm) bottom fresh alfalfa leaf or goosefoot leaf on, full skirt after mating 1d being chased after and posts the female fly of fly and put into and connect fly case, will 15%(volume fraction be soaked with) rayon balls of the hydromel end face that hangs over fly case chases after the food source of posting fly adult as full skirt.Remove full skirt after 24h to chase after and post fly, beet webworm is proceeded in vial respectively and continues to raise with fresh alfalfa leaf or goosefoot leaf, 30 every bottle.Full skirt is chased after and posts the fly worm phase and be divided into ovum, low instar larvae (1-3 instar larvae), high instar larvae (4-5 instar larvae) and adult four-stage, from the ovum phase, by different treatment combination designing requirements (see table 2), will alternately be placed in corresponding photoperiod environment for examination worm in time.Observe record different disposal full skirt and chase after the diapause situation of posting fly, thus determine that full skirt chases after the sensitive period posting fly Diapause inducement.
Result is as shown in table 2, known full skirt chases after the crucial life stages sensitive that the larval phase of posting fly is its diapause, be larval phase to the sensitive period of photoperiod irritant reaction, low instar larvae (1-3 instar larvae) or high instar larvae (4-5 instar larvae) all can experience the inducing action of short photoperiod to diapause.The ovum phase and the Diapause inducement effect of adult stage to short photoperiod insensitive.Under only having larva to be in short photoperiod condition, larva just can enter diapause; After larval phase accepts short photoperiod process continuously, its Diapause rate obviously increases, and shows that the photoperiod stimulates and has summation.The short photoperiod experience of ovum phase occurs also there is certain facilitation to the diapause of larva.
Table 2 full skirt chases after posts the sensitive period that fly different developmental phases experiences diapause signal
| Process | The ovum phase | 1-3 instar larvae | 4-5 instar larvae | Just incubate adult | Observe borer population (head) | Diapause borer population (head) | Diapause rate (%) |
| 1 | S | L | L | L | 47 | 3 | 6.4 |
| 2 | L | S | L | L | 53 | 30 | 56.6 |
| 3 | L | L | S | L | 49 | 25 | 51.0 |
| 4 | L | L | L | S | 46 | 2 | 4.3 |
| 5 | L | L | L | L | 55 | 4 | 7.3 |
| 6 | S | S | L | L | 51 | 25 | 49.0 |
| 7 | S | S | S | L | 41 | 31 | 75.6 |
| 8 | S | S | S | S | 53 | 50 | 94.3 |
| 9 | L | S | S | S | 51 | 47 | 92.2 |
| 10 | L | L | S | S | 49 | 29 | 59.2 |
| 11 | L | S | S | L | 48 | 41 | 85.4 |
Note: long illumination (L, the photoperiod is L16:D8) and short photoperiod (S, the photoperiod is L10:D14).
Embodiment 2, full skirt chase after the diapause maintenance posting fly
1, full skirt chases after the eclosion rate posting fly
The full skirt of the diapause state adopting the method for embodiment 1 to obtain is chased after and posts fly larva (parasitize pupated beet webworm body in) and be positioned in the plastic containers filling moist fine sand, then put it in 4 ± 2 DEG C, refrigerator, preserve under full dark condition, to spray water every day moisturizing, maintain relative air humidity 70%.The beet webworm cocoon regularly having diapause state full skirt to chase after parasitism to post fly larva takes out and puts into temperature 23 ± 1 DEG C from refrigerator, the incubator of photoperiod L16: D8 carries out full skirt and chases after the emergence of posting fly, observes and adds up the eclosion rate refrigerating parasitic fly after different time.8 process (8 cold preservation times, in table 3) are established in experiment altogether, and each process 50-70 parasitism has diapause state full skirt to chase after the beet webworm cocoon posting fly larva.The beet webworm cocoon simultaneously having non-diapause state full skirt to chase after to post fly larva with the parasitism of non-chilling treatment is in contrast (CK). experiment repetition 5 times, results averaged.
2, the full skirt after sprouting wings chases after the life-span of posting fly
Full skirt after emergence step 1 obtained chases after to be posted fly and becomes fly to put into insect box, every box is introduced a pair full skirt and is chased after and post fly (the male fly of 1 female fly+1), with the nutrition as a supplement of the hydromel of mass fraction 15%, mating 24h under the condition of temperature 23 DEG C, photoperiod L16: D8, then inoculate, chase after by the full skirt after mating 1d and post the female fly of fly and parasitize beet webworm larva.Repeat 3 times.Often pair of fly inoculates 30 4-5 beet webworm in age larvas for female fly parasitism, takes out male and female fly and continue inoculation, until the death of male and female fly after 24h.Statistics full skirt chases after the life-span of the one-tenth fly after posting fly emergence.
3, result
Result is as shown in table 3, finds that the storage time long full skirt that can significantly reduce chases after the eclosion rate posting fly.Full skirt without storage chases after and posts fly eclosion rate is 85.2%.Along with the passing in storage time, full skirt chases after the eclosion rate posting fly obviously to be reduced, and between the eclosion rate in the different storage times of " 0-150 days ", there is pole significant difference (p < 0.01), female, male fly life-span and control group have significant difference (P>0.05), and illustrating parasitic has diapause state full skirt to chase after to post beet webworm cocoon low temperature storage 30d, the 60d of fly larva to chase after full skirt to post fly eclosion rate, life-span, do not have considerable influence.Above result show to parasitism have diapause state full skirt chase after the beet webworm cocoon posting fly larva do short-term store 30-60d comparatively suitable
Table 3 low temperature storage chases after the impact of posting fly on full skirt
Note: in table, data (mean value ± standard error) newly answer range test through DuncanShi, after each row of data, different capitalization represents that difference is extremely significantly (P < 0.01); Different lowercase alphabet shows significant difference (P < 0.05).
Embodiment 3, full skirt chase after the Diapause termination posting fly
Full skirt for the diapause state adopting the method for embodiment 1 to obtain chases after posts fly, and by thermal stimulus, termination of diapause state, enters development process, and concrete measure is as follows:
Parasitism is had diapause state full skirt chase after the beet webworm cocoon posting fly larva be placed in 23 DEG C, photoperiod L14:D10, luminous intensity 5000Lx environment, spray a water every day, keep certain humidity (RH is 70%), full skirt can be made after 20 days to chase after and post fly larva termination of diapause state, all sprouting wings very soon into fly.
Wherein, induction time is determined for 20 days as follows:
To the method for embodiment 2 storage parasitism after 60 days under complete dark, 4 DEG C of conditions be adopted to have diapause state full skirt to chase after incubator (luminous intensity 5000Lx that the beet webworm pupa posting fly larva proceeds to temperature 23 DEG C, photoperiod L14: D10, RH is 70%) in carry out Diapause termination, the emergence time of statistics parasitic fly, adopt 50-60 parasitism to have diapause state full skirt to chase after the beet webworm cocoon posting fly larva to test, establish altogether and repeat experiment for 3 times.Result shows, and starts to carry out Diapause termination from having parasitism diapause state full skirt to chase after to post the beet webworm cocoon of fly larva under proceeding to 23 DEG C of conditions, and all sprouting wings very soon after about 20 days into fly, termination of diapause state.Repeat experimental result indifference three times.
Claims (4)
1. regulate and control full skirt and chase after a method of posting fly diapause, comprise the steps:
(1) full skirt of pending Diapause inducement is chased after post under fly is placed in the condition of temperature 17 ± 1 DEG C, photoperiod L8:D16 to L12:D12 and induce, described full skirt is chased after post fly to enter diapause state;
(2) the described full skirt entering diapause state that step (1) obtains is chased after post under fly proceeds to temperature 4 DEG C, dark condition and store;
(3), after step (2) storage terminates, described full skirt is chased after and posts under fly proceeds to the condition of temperature 23 ± 1 DEG C, photoperiod L14:D10 to L16:D8, recover to grow;
The full skirt of described pending Diapause inducement chases after and posts fly is that full skirt chases after the ovum or larva of posting fly; Described full skirt is chased after and posts time that fly carries out Diapause inducement and at least contain described full skirt and chase after the larval phase of posting fly.
2. method according to claim 1, is characterized in that: in step (2), and the time of described storage is within 120 days.
3. method according to claim 2, is characterized in that: in step (2), and the time of described storage is within 60 days.
4. method according to claim 1, is characterized in that: in step (3), and the described time of recovering to grow is 20 days.
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| CN104351140B (en) * | 2014-09-24 | 2017-10-17 | 中国农业科学院植物保护研究所 | Red shoulder ladybug Diapause termination method |
| CN105379685B (en) * | 2015-11-25 | 2017-10-10 | 云南农业大学 | A kind of method of the Japanese Exorista civilis pupa of preservation |
| CN105660541B (en) * | 2016-02-15 | 2019-03-01 | 山东省农业科学院植物保护研究所 | A method of induction spotted wing drosophila reproductive diapause |
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| UA25135U (en) * | 2007-03-27 | 2007-07-25 | Univ Nat Agrarian | Method to form a wintering reserve of species of the genus trichogramma |
| CN102206263A (en) * | 2011-03-23 | 2011-10-05 | 吉林省蚕业科学研究院 | Tussah antibiotic extraction method based on tussah pupa in vivo induction |
| CN102771445A (en) * | 2012-08-07 | 2012-11-14 | 中国农业科学院植物保护研究所 | Diapause induction and cancellation method of natural enemy insect |
| CN102771448A (en) * | 2012-08-07 | 2012-11-14 | 中国农业科学院植物保护研究所 | Natural enemy insect (coccinella septempunctata) diapause induction method |
| CN103141443A (en) * | 2013-03-08 | 2013-06-12 | 中国农业科学院草原研究所 | Method for regulating and controlling diapause of agryponflexorius |
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