CN103478486A - Diamondback moth larva pure artificial feed and preparation method thereof - Google Patents
Diamondback moth larva pure artificial feed and preparation method thereof Download PDFInfo
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- CN103478486A CN103478486A CN201310411615.9A CN201310411615A CN103478486A CN 103478486 A CN103478486 A CN 103478486A CN 201310411615 A CN201310411615 A CN 201310411615A CN 103478486 A CN103478486 A CN 103478486A
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Abstract
The present invention specifically discloses a diamondback moth larva pure artificial feed and a preparation method thereof. The feed contains malt powder, casein, sucrose, a multivitamin mixture, cellulose, agar and other auxiliary materials, and is determined through comparison and optimization. According to the present invention, the feed contains nutrients required by health growth of diamondback moth larva, and the ratio of various components is within a normal range; the feed is not added with vegetable leaf powder components, such that no pesticide residue in the feed is ensured, and the fed diamondback moth is the pure line sensitive species; and with the feed, a lot of diamondback moth polypides with characteristics of uniform development and the same instar can be obtained, and scientific research requirements can be completely met.
Description
Technical field
The present invention relates to the technique for producing feed field, particularly, relate to pure man-made feeds of a kind of diamondback moth larvae and preparation method thereof.
Background technology
Diamondback moth (
plutella xylostella) claim again the hang spring worm, belong to Lepidoptera diamond-back moth section, be one of primary pest of worldwide brassicaceous vegetable, the crops such as the wild cabbage of main harm Cruciferae class, Chinese cabbage, radish.Diamondback moth gnaws blade, cause incise, hole, when serious, whole leaf is eaten up, and agricultural production has been produced to huge economic loss.It is many that generation occurs in this worm year, generation overlap, and medicament is easily developed immunity to drugs, be that in current production, chemical insecticide is prevented and treated one of difficult typical insect.In order to overcome and to prevent and treat the harm that diamondback moth brings, for a long time, people prevent and treat with various chemical agents, but because medication is frequent, add the diamondback moth particularity of the history of life, it has all produced the resistance to the action of a drug in various degree to various pesticides, to cause many pesticides in succession to be lost or to have weakened prevention effect.Diamondback moth is one of the most serious insect of current development of drug resistance.
In order to carry out the research to the Biological Control of Plutella xylostella aspect, the researcher needs a large amount of diamondback moth polypides as research material.The research material diamondback moth mainly is divided into responsive system and resistance system.Responsive system diamondback moth needs artificial feeding in enormous quantities.At present, known diamondback moth laboratory rearing method commonly used has semi-synthetic diet method, the continuous method for breeding of cabbage seedling, radish seedling method, germination vegetable seed method for breeding etc.The shortcoming of these methods is that the diamondback moth feeding efficiency is low, need specific environment and a large amount of artificial inputs, Human disturbance is large, the diamondback moth survival rate is low, operation is comparatively complicated, and general laboratory can't be fully satisfied, and it is uneven easily to occur in feeding process that polypide grows, worm inconsistent phenomenon in age, thus cause result of study inaccurate.
Summary of the invention
The present invention is in order to overcome diamondback moth method for breeding complexity in prior art, to have high input, and the polypide growth is uneven, and worm inconsistent defect in age, provide a kind of diamondback moth pure man-made feeds.
Another object of the present invention is to provide the preparation method of the pure man-made feeds of a kind of diamondback moth.
Another object of the present invention is to provide the method for breeding of a kind of diamondback moth.
The present invention's above-mentioned purpose that is achieved by the following technical programs
The pure man-made feeds of a kind of diamondback moth larvae, each component that described feed contains following percetage by weight: malt flour 5.0～5.5%, casein 2.0～3%, sucrose 4.0～4.5%, Muhivitamin Formula With Minerals mixture 2.0%, cellulose 8.0%, agar 2.0%, water 75～80%, antioxidant 0.02%, anticorrisive agent 0.785%, ascorbic acid 0.375% and linseed oil 0.32%.
From above-mentioned feed formula, the major ingredient in feed is: malt flour 5.0～5.5%, casein 2.0～3%, sucrose 4.0～4.5%, cellulose 8.0%, agar 2.0%, water 75～80%; Auxiliary material is Muhivitamin Formula With Minerals mixture 2.0%, antioxidant 0.02%, anticorrisive agent 0.785%, ascorbic acid 0.375% and linseed oil 0.32%.
Preferably, each component that the pure man-made feeds of described diamondback moth larvae contain following percetage by weight: malt flour 5.0%, casein 2.5%, sucrose 4.0%, Muhivitamin Formula With Minerals mixture 2.0%, cellulose 8.0%, agar 2.0%, water 75%, antioxidant 0.02%, anticorrisive agent 0.785%, ascorbic acid 0.375% and linseed oil 0.32%.
Preferably, described antioxidant is a kind of in n-propyl gallate, dibutyl hydroxy toluene, ditert-butylhydro quinone.
Preferably, described anticorrisive agent is at least two kinds in tetracycline, methyl p-hydroxybenzoate, potassium sorbate or formaldehyde.
More preferably, each component that described feed contains following percetage by weight: malt flour 5.0%; Casein 2.5%; Sucrose 4.0%; Muhivitamin Formula With Minerals mixture 2.0%; Cellulose 8.0%; Agar 2.0%; Water 75%; N-propyl gallate 0.02%; Tetracycline 0.125%; Methyl p-hydroxybenzoate 0.1%; Ascorbic acid 0.375%; Potassium sorbate 0.4%; Formaldehyde 0.16% and linseed oil 0.32%.
These man-made feeds not only needn't be changed feed and take out the worm excrement in feeding process, have simplified and have educated the worm management work, have greatly shortened breeding cycle, a large amount of diamondback moth polypides can be provided in the short period of time, thereby meet in time the needs of scientific research.And, utilize feed of the present invention to raise diamondback moth larvae and can obtain that a large amount of growths are neat, worm consistent polypide in age.
Described Muhivitamin Formula With Minerals mixture is that commercially available 21 Jin Weita Muhivitamin Formula With Minerals slice lappings form.
Beneficial effect of the present invention:
(1) diamondback moth larvae feed of the present invention is on forefathers' Research foundation, by contrast and optimization, establishes.At first, feed comprises the needed nutrition of diamondback moth larvae healthy growth, and each component ratio is in a normal range (NR); Secondly, feed does not add dish leaf meal component, has guaranteed in feed not exist Pesticide Residue, and the diamondback moth of raising is planted for pure lines are responsive.
(2), in the diamondback moth larvae feeding process, the pupa of collection and ovum can, by of short duration stored refrigerated, not only can be unified diamondback moth and grow the length of time, and can reduce artificial input.
(3) in the diamondback moth feeding process, while utilizing the nest of pupating not only can reduce artificial secondary transferring pupa, the injury caused to pupa, and improved the efficiency that diamondback moth pupates; Utilization is laid eggs card not only can reduce artificial secondary transferring ovum the time, the injury caused to ovum, and reduced the loss quantity of Oviposition of Diamondback Moth.
The specific embodiment
Further describe the present invention below in conjunction with specific embodiment.Unless stated otherwise, the reagent adopted in embodiment and method are conventional reagent and the method for using in this area.
The embodiment pure man-made feeds of 1 diamondback moth and preparation method
The formula of the pure man-made feeds of diamondback moth is as follows:
Wherein, the Muhivitamin Formula With Minerals mixture is that commercially available 21 Jin Weita Muhivitamin Formula With Minerals slice lappings form.
The pure man-made feeds of diamondback moth the preparation method:
S1. take malt flour, casein, sucrose, cellulose, methyl p-hydroxybenzoate, n-propyl gallate, potassium sorbate by above-mentioned formula, put into mixer container.
S2. take tetracycline, formaldehyde, linseed oil, Muhivitamin Formula With Minerals mixture, ascorbic acid by above-mentioned formula, put into plastic containers A, stir and evenly mix with glass bar.
S3. take 20g agar by above-mentioned formula and pour in the pot that 1000mL water is housed, with glass bar, stir and make agar mix with water.Pot is placed on electromagnetic oven and heats, with glass bar, ceaselessly stir agar solution.When transparent glue appears in the edge of pot by the time, use moderate heat instead, accelerate the mixing speed of glass bar.While by the time coming to life in the middle of the agar solution in the pot, use little fighting instead and ceaselessly stir a period of time with glass bar, then close fire.
The agar solution of the heat S4. step S3 prepared joins in the mixer container that fills material in step S1, stir 1 minute, when in mixer container, solution temperature is lower than 70 ℃, the material that adds step S2 to take (high temperature can destroy the action effect of composition in S2).Cover the mixer container cover, stir 1～2 minute.Rapidly feed is poured in lunch box.
The lunch box that S5. will fill feed is placed on indoor cooling 1～2 hour, cover lid, 4 ℃ of preservations.
embodiment 2the pure man-made feeds of diamondback moth and preparation method
The formula of the pure man-made feeds of diamondback moth is as follows:
Wherein, the Muhivitamin Formula With Minerals mixture is with embodiment 1.
The pure man-made feeds of diamondback moth the preparation method: with embodiment 1.
embodiment 3the pure man-made feeds of diamondback moth and preparation method
The formula of the pure man-made feeds of diamondback moth is as follows:
Wherein, the Muhivitamin Formula With Minerals mixture is with embodiment 1.
The pure man-made feeds of diamondback moth the preparation method: with embodiment 1.
embodiment 4the pure man-made feeds of diamondback moth and preparation method
The formula of the pure man-made feeds of diamondback moth is as follows:
Wherein, the Muhivitamin Formula With Minerals mixture is with embodiment 1.
The pure man-made feeds of diamondback moth the preparation method: with embodiment 1.
embodiment 5the described feed of Application Example 1 carries out the cultivation of the indoor subculture of diamondback moth, and (the cabbage mustard seedling is as CK also to adopt in prior art several diamondback moth feeds commonly used simultaneously; The patent No. is the described feed of 200810058886.X as a comparison case 1; The 83-28 feed that Fang Julian etc. (1988) filter out is Comparative Examples 2) with feed of the present invention, compare.
Method for breeding is specific as follows:
S1. the collection of the raising of diamondback moth larvae and pupa: the vessel of raising use are jelly cup or plastic casing, rim of a cup seals with preservative film, with rubber band, tighten, evenly prick hole with insect needle on preservative film, pitch of holes is 1 cm left and right, until on vessel wall and after the aqueous vapor disappearance on feed (embodiment 1 feed or comparison feed) surface, access the ovum through sterilizing or access newly hatched larvae with writing brush.Cuvette connects 10 of 10, ovum or newly hatched larvaes, and mug connects 100 of 100, ovum or newly hatched larvaes, puts into growth cabinet and raises, and temperature is 24～26 ℃, relative humidity 30-50%, and periodicity of illumination is 13L～11D.When larval growth to 4 age Mo, to putting 1～2 diamondback moth nest of pupating in foster worm cup, mature larva is pupated on the nest of pupating, and collects in time pupa.The pupa of collecting can directly be put to sprouting wings in dependent insect cage and lay eggs, and also can put to bottom, to be lined with in the culture dish of moistening filter paper stored refrigerated standbyly under 4～5 ℃, in 20 d, its eclosion rate is not made significant difference.
S2. the collection of the raising of diamondback moth adult and ovum: 80～100 of well-developed pickles chrysalis are put into to dependent insect cage (30cm # 20cm # 15cm), nail on drawing pin the cotton balls that the libation at an ancient wedding ceremony has 10% Glucose Liquid in cage, hang the card (made by green blowing paper, dry rear standby with the immersion of rape juice) of laying eggs in cage.Dependent insect cage is put into to illumination box, and after 2～4 days, the pickles chrysalis starts emergence, mating, lays eggs, and changes subsequently the card of laying eggs every day to collect ovum, and drips 10% hydromel for Adult supplement nutrient.The ovum card of collecting can directly be put on the described man-made feeds of embodiment 1 hatches, and also can be put into bottom and be lined with in the plastic casing of moistening filter paper and hide and save backup 4～5 ℃ of refrigerator and cooled, in 20 d, its incubation rate is not made significant difference.
Through the test statistics analysis, with feed of the present invention with diamondback moth larvae result with several diamondback moth feeds commonly used are raised in prior art as following table 1.
The diamondback moth larvae result that in table 1. embodiment 1 feed and use prior art, several diamondback moth feeds commonly used are raised
1), raising condition: temperature 25-27 ℃, relative humidity 30-50%, every part of feed is thrown and is supported 200, repeats 3 times; 2), adopt the least significant difference (LSD) minute in one-dimensional variance analysis (One-Way ANOVA)
Analyse, multiple ratio adopts the DuncanShi method of inspection.Mark same letter person is illustrated in variance analysis difference not significantly (P>0.05) in same column, marks different alphabetical persons and is illustrated in significant difference in variance analysis (P<0.05).
Conclusion: utilize the effect aspect of feed raising diamondback moth of the present invention slightly to raise inferior to the cabbage mustard seedling, but feed is diamondback moth, extensive raising the in laboratory simplified flow process, saved the labour, for the large-scale production diamondback moth provides possibility.Utilize optimization of C/C composites feed of the present invention to raise diamondback moth, newly hatched larvae is 12.3 days to the phase of going through of adult, and pupation probability is 54.3%, the emergence probability is 93.2%, and the adult behavior is normal, female worm average life span 9.5 d, male worm 13.5 d, 107 of the average egg laying amounts of every female worm; The 83-28 feed filtered out in (1988) such as the effect aspect the eclosion rate of percentage of pupation and pupa and Comparative Examples 3(side chrysanthemum lotuses) compare better; With the Comparative Examples 2(patent No., be the described feed of 200810058886.X) to compare, feed of the present invention is short aspect going through the phase larva.In sum, diamondback moth larvae optimization of C/C composites feed of the present invention, composition is simple, easy operating, Human disturbance is few, and feeding efficiency is high, and feed does not add dish leaf meal component, guaranteed in feed not exist Pesticide Residue, the diamondback moth of raising is planted for pure lines are responsive.
Claims (6)
1. pure man-made feeds of diamondback moth larvae, it is characterized in that each component that described feed contains following percetage by weight: malt flour 5.0～5.5%, casein 2.0～3%, sucrose 4.0～4.5%, Muhivitamin Formula With Minerals mixture 2.0%, cellulose 8.0%, agar 2.0%, water 75～80%, antioxidant 0.02%, anticorrisive agent 0.785%, ascorbic acid 0.375% and linseed oil 0.32%.
2. pure man-made feeds of diamondback moth larvae according to claim 1, it is characterized in that each component that described feed contains following percetage by weight: malt flour 5.0%, casein 2.5%, sucrose 4.0%, Muhivitamin Formula With Minerals mixture 2.0%, cellulose 8.0%, agar 2.0%, water 75%, antioxidant 0.02%, anticorrisive agent 0.785%, ascorbic acid 0.375% and linseed oil 0.32%.
3. according to the pure man-made feeds of the described diamondback moth larvae of claim 1 or 2, it is characterized in that, described Muhivitamin Formula With Minerals mixture is that commercially available 21 Jin Weita Muhivitamin Formula With Minerals slice lappings form.
4. according to the pure man-made feeds of the described diamondback moth larvae of claim 1 or 2, it is characterized in that, described antioxidant is a kind of in n-propyl gallate, dibutyl hydroxy toluene or ditert-butylhydro quinone.
5. according to the pure man-made feeds of the described diamondback moth larvae of claim 1 or 2, it is characterized in that, described anticorrisive agent is at least two kinds in tetracycline, methyl p-hydroxybenzoate, potassium hydroxide or formaldehyde.
6. according to the described feed of claim 1 to 5 any one, it is characterized in that each component that described feed contains following percetage by weight: malt flour 5.0%; Casein 2.5%; Sucrose 4.0%; Muhivitamin Formula With Minerals mixture 2.0%; Cellulose 8.0%; Agar 2.0%; Water 75%; Vitamin mixtures 1.0%; N-propyl gallate 0.02%; Tetracycline 0.125%; Methyl p-hydroxybenzoate 0.1%; Ascorbic acid 0.375%; Potassium sorbate 0.4%; Formaldehyde 0.16% and linseed oil 0.32%.
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Cited By (4)
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| CN104666341A (en) * | 2015-01-30 | 2015-06-03 | 上海华源安徽锦辉制药有限公司 | Multivitamin and mineral tablet and mixing granulation technology thereof |
| CN105010753A (en) * | 2015-08-14 | 2015-11-04 | 山东省农业科学院植物保护研究所 | Artificially-synthesized feed for athetis dissimilis hampson and preparation method of artificially-synthesized feed |
| US20180368444A1 (en) * | 2016-10-25 | 2018-12-27 | Guangdong Institute Of Applied Biological Resources | Artificial diet for ghost moth larvae and preparation method thereof |
| CN110089642A (en) * | 2019-06-15 | 2019-08-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Spodopterafrugiperda man-made feeds |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104666341A (en) * | 2015-01-30 | 2015-06-03 | 上海华源安徽锦辉制药有限公司 | Multivitamin and mineral tablet and mixing granulation technology thereof |
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| US20180368444A1 (en) * | 2016-10-25 | 2018-12-27 | Guangdong Institute Of Applied Biological Resources | Artificial diet for ghost moth larvae and preparation method thereof |
| US10681921B2 (en) * | 2016-10-25 | 2020-06-16 | Guangdong Institute Of Applied Biological Resources | Artificial diet for ghost moth larvae and preparation method thereof |
| CN110089642A (en) * | 2019-06-15 | 2019-08-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Spodopterafrugiperda man-made feeds |
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Application publication date: 20140101 |