CN106106375A - A kind of method of water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth - Google Patents
A kind of method of water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth Download PDFInfo
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- CN106106375A CN106106375A CN201610505668.0A CN201610505668A CN106106375A CN 106106375 A CN106106375 A CN 106106375A CN 201610505668 A CN201610505668 A CN 201610505668A CN 106106375 A CN106106375 A CN 106106375A
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- 238000000034 method Methods 0.000 title claims abstract description 60
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 241000500437 Plutella xylostella Species 0.000 title claims abstract description 57
- 230000000384 rearing effect Effects 0.000 title claims abstract description 13
- 235000013601 eggs Nutrition 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 17
- 239000000758 substrate Substances 0.000 claims description 12
- 230000012010 growth Effects 0.000 claims description 10
- 241000382353 Pupa Species 0.000 claims description 9
- 230000032669 eclosion Effects 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 8
- 102000002322 Egg Proteins Human genes 0.000 claims description 6
- 108010000912 Egg Proteins Proteins 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 210000004681 ovum Anatomy 0.000 claims description 6
- 229930192334 Auxin Natural products 0.000 claims description 5
- 239000002363 auxin Substances 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 5
- 239000010413 mother solution Substances 0.000 claims description 5
- 235000019362 perlite Nutrition 0.000 claims description 4
- 239000010451 perlite Substances 0.000 claims description 4
- 239000012286 potassium permanganate Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 3
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 238000007865 diluting Methods 0.000 claims description 2
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 2
- 239000013505 freshwater Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 238000011017 operating method Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 14
- 235000011293 Brassica napus Nutrition 0.000 abstract description 9
- 240000008100 Brassica rapa Species 0.000 abstract description 9
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 abstract description 9
- 241000500441 Plutellidae Species 0.000 abstract 1
- 239000000090 biomarker Substances 0.000 abstract 1
- 238000009395 breeding Methods 0.000 description 14
- 230000001488 breeding effect Effects 0.000 description 14
- 240000007124 Brassica oleracea Species 0.000 description 10
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 7
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 7
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 7
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- 238000011161 development Methods 0.000 description 4
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- 230000002786 root growth Effects 0.000 description 4
- 235000013311 vegetables Nutrition 0.000 description 4
- 235000011302 Brassica oleracea Nutrition 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 238000010413 gardening Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 235000021110 pickles Nutrition 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000628997 Flos Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- OQVYMXCRDHDTTH-UHFFFAOYSA-N 4-(diethoxyphosphorylmethyl)-2-[4-(diethoxyphosphorylmethyl)pyridin-2-yl]pyridine Chemical compound CCOP(=O)(OCC)CC1=CC=NC(C=2N=CC=C(CP(=O)(OCC)OCC)C=2)=C1 OQVYMXCRDHDTTH-UHFFFAOYSA-N 0.000 description 1
- 241000242759 Actiniaria Species 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 235000005637 Brassica campestris Nutrition 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 244000178937 Brassica oleracea var. capitata Species 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241001301148 Brassica rapa subsp. oleifera Species 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- PNBTUQWEXQHLNJ-UHFFFAOYSA-N C1(=CC=CC2=CC=CC=C12)CC(=O)O.C(C)(=O)O.C1=CC=CC2=CC=CC=C12 Chemical compound C1(=CC=CC2=CC=CC=C12)CC(=O)O.C(C)(=O)O.C1=CC=CC2=CC=CC=C12 PNBTUQWEXQHLNJ-UHFFFAOYSA-N 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 241000241602 Gossypianthus Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 241000819999 Nymphes Species 0.000 description 1
- 241000907661 Pieris rapae Species 0.000 description 1
- 231100000605 Toxicity Class Toxicity 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 235000021405 artificial diet Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
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- 235000013305 food Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
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- 230000017448 oviposition Effects 0.000 description 1
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- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a kind of method that water planting Caulis et Folium Brassicae capitatae raises diamondback moth, under diamondback moth rearing conditions, temperature 24 DEG C, relative humidity 70%, photoperiod L: D=16: 8, commodity Caulis et Folium Brassicae capitatae is carried out water planting, take root good, individual plant water planting Caulis et Folium Brassicae capitatae can be used for the raising of one complete generation of 200 300 diamondback moths, and the diamondback moth that each Biological indicators of diamondback moth of raising are raised with potted plant Caulis et Folium Brassicae capitatae, Vermiculitum Turnip Sprouts method is not significantly different from.The method is simple to operate, low cost, and feedstuff is supplied at any time, and the mass rearing making indoor diamondback moth is more convenient.
Description
One, technical field
The method that the present invention relates to a kind of water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth.
Two, background technology
Diamondback moth Plutella xylostella (L.) belongs to Lepidoptera, diamond-back moth section, another name: little Kattapillar, Rhizoma Anemones Raddeanae, hang spring
Worm, 40 kinds of crop in cruciferae such as main harm Caulis et Folium Brassicae capitatae, broccoli, Caulis et Folium Brassicae junceae, Brassica oleracea L. var. botrytis L., Chinese cabbage, Brassica campestris L, Radix Raphani, are worldwide ten
One of important pests of Zi Hua section vegetable (Feng Xia etc., 2011), has the ability (Xing Kun etc., 2015) migrated at a distance.Pickles
The features such as moth distribution is wide, and reproductive capacity is high, and biocycle is short, generation overlap, development of drug resistance fast (Liang Pei etc., 2001), become elder brother
The object of study (Yang Fengshan, 2004) that the fields such as worm Drug resistance, physiology, pathology, toxicology are important, is that laboratory insecticide is ground
Study carefully, biological pesticide toxicity evaluation test standard worm kind (Lin Kun, 2011).
It is the basis carrying out the every research of diamondback moth that the indoors artificial of diamondback moth Plutella xylostella L is raised.
At present, the method that diamondback moth laboratory rearing is conventional has Ke Lidao and side chrysanthemum lotus (1981) germination Semen Allii Tuberosi method, Liu Chuanxiu etc.
(1981) by Turnip Sprouts Vermiculitum method, the method that both is raised with seedling, feedstuff easily obtains, but the quantity of food that seedling provides has
Limit, it is difficult in mass rearing, and diamondback moth larvae growth and development process, often, part nymph can not shift in switching, and worm amount is damaged
Losing big, and easily catch an illness, after pupating, pupa inconvenience high-volume is collected and is preserved.Chen Zongqi etc. (2001) utilize Flos Nelumbinis in vain as pickles
The feedstuff of moth, Yang Fengshan etc. (2004) raises diamondback moth with cabbage seedling, can be grown neat for examination worm source in a large number, but post
The main plant culture phase is long, is easily endangered by other insect such as worm such as Pieris rapae, aphid, difficult management, climate, soil, place etc.
The restriction of condition, feedstuff can not be supplied in time, cultivates cost high.Xu Qin (2005), Mo Meihua (2007) use artificial diet
Diamondback moth, needs special formula, operates more complicated, and general laboratory uses certain limitation.Lin Kun etc.
(2011) by green vegetable Turnip Sprouts method, diamondback moth lays eggs on green vegetable Turnip Sprouts, raises with the cabbage leaves bought back on market after egg hatching,
Need 2d to change 1 time, it is ensured that the freshness difficulty of dish leaf, transfer frequently, unfavorable and the most susceptible to diamondback moth growth.
Three, summary of the invention
In order to solve to raise in prior art the various problems of diamondback moth, the present invention is on the basis of forefathers study, comprehensively
Above-mentioned method for breeding, explores and utilizes the Caulis et Folium Brassicae capitatae bought back on market, after it is carried out a series of process, raises for diamondback moth
Method.The method for breeding of a kind of low cost simple will be provided herein for indoor a large amount of continuously rearing diamondback moth.
Concrete, the technical solution used in the present invention is:
The method of a kind of water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth, said method comprising the steps of:
(1) host plant is cultivated
Choose the Caulis et Folium Brassicae capitatae that robust growth, balling are smooth, divest residual leaf, 1/3rd of crosscutting removing leaf-head top, then cut
Remove the part rhizome of bottom, expose fresh rhizome tissue, by the bottom notches of the stem ethanol water of volume fraction 75%
After soaking 1 minute, take out and put in aseptic cup, process rhizome otch 10 minutes with mass fraction 0.1% potassium permanganate solution,
Again with after aseptic water washing 2-3 time, then the bottom notches of stem being stained with auxin solution 30 seconds, taking-up is dried, after being processed
Leaf-head；Described auxin solution is prepared by the following method: naphthalene acetic acid is dissolved in the ethanol of volume fraction 95% preparation and obtains
The mother solution of 1mg/ml concentration, then by mother solution with distilled water diluting to concentration 1000ppm；
Leaf-head after processing is put in the culture vessel equipped with water or the substrate containing saturation water, and the bottom notches making stem is deep
0.5-1cm under upstream face, gained water planting Caulis et Folium Brassicae capitatae i.e. can be used for diamondback moth and raises；Described substrate is Vermiculitum or perlite.
(2) diamondback moth is raised
Diamondback moth 50～150 pupas (preferably 100 pupas) are put into case of laying eggs, at temperature 24 ± 1 DEG C, relative humidity
70%, cultivate under conditions of photoperiod L:D=18:8 and sprout wings, after more than 50% adult eclosion, the water planting Caulis et Folium Brassicae capitatae of step (1)
Put into 24h of laying eggs in case of laying eggs, take out, connect the water planting Caulis et Folium Brassicae capitatae after ovum in growth cabinet, at temperature 24 DEG C, relative humidity
70%, diamondback moth is raised under conditions of photoperiod L: D=16: 8, intensity of illumination 4000lx to pupating.
The repeatable operating procedure of diamondback moth (2) after pupating, cultivates to more than 50% adult eclosion in case of laying eggs, then
Putting into fresh water planting Caulis et Folium Brassicae capitatae to carry out laying eggs, raising, each water planting Caulis et Folium Brassicae capitatae can be used for one complete generation of 200-300 head diamondback moth
Raising.
In described step (1), the leaf-head after preferably processing is put in the culture vessel of dress water, and the bottom notches making stem is deep
0.5-1cm under upstream face, gained water planting Caulis et Folium Brassicae capitatae is raised for diamondback moth.Directly it is better than substrate by Aquaponic effect to cultivate.
Caulis et Folium Brassicae capitatae (Brassica oleracea L.) used by the present invention, directly can buy in market and obtain, generally knot
Cabbage is also known as cabbage, cabbage, Caulis et Folium Brassicae capitatae etc..
In described step (1), preferably changed 1 water every 3 days, further, after water planting Caulis et Folium Brassicae capitatae starts long root, change water every time
Time add nutritional solution, nutritional solution stock solution obtain according to volume ratio 1:1000 mixed preparing with water.Described nutritional solution stock solution can be straight
Connecing purchase to obtain, all kinds of plants or fruit vegetable nutrient liquid are used equally to the present invention, use Shi Ke get garden in the embodiment of the present invention
The home gardening concentrated nutrient solution that skill Products Co., Ltd produces.
The present invention utilizes the Caulis et Folium Brassicae capitatae bought back on market, to its carry out a series of process cultivate after, obtain water planting Caulis et Folium Brassicae capitatae for
Raise diamondback moth to raise, can obtain and preferably raise result, the pickles chrysalis weight 7.15mg of raising, the eclosion rate 91.85% of pupa,
List is female lays eggs 86.70, the incubation rate 94.32% of ovum, and the diamondback moth raised with potted plant Caulis et Folium Brassicae capitatae, Vermiculitum Turnip Sprouts method has significance difference
Different.The method is simple to operate, low cost, and the cultivation phase is short, and feedstuff is supplied at any time, facilitates the mass rearing of indoor diamondback moth.
The inventive method is with low cost, and operation is simple, it is possible to ensure normal development and the breeding of diamondback moth, with the most potted plant
Plant culturing is compared saving of labor, is saved time, and the cultivation phase is short, cultivates low cost, and the condition such as climate, soil does not limits, more convenient.
Four, accompanying drawing explanation
Fig. 1 water planting Caulis et Folium Brassicae capitatae processes, raises the procedure chart of diamondback moth, from left to right, the most untreated Caulis et Folium Brassicae capitatae, excision leaf
/ 3rd and a Caulis et Folium Brassicae capitatae of part rhizome on ball top, at a temperature of 24 DEG C cultivate take root 10 days Caulis et Folium Brassicae capitatae, raise diamondback moth sweet
Blue.
The comparison diagram that under Fig. 2 different temperatures, water planting Caulis et Folium Brassicae capitatae root growth was taken root after 20 days.
Fig. 3 raises diamondback moth instance graph with different method for breeding.
Five, detailed description of the invention
With specific embodiment, technical scheme is described further below, but protection scope of the present invention is not
It is limited to this.
Embodiment 1
1 materials and methods
1.1 materials and equipment
Caulis et Folium Brassicae capitatae (Brassica oleracea L.), buy marketed common head cabbage (also known as cabbage, cabbage,
Caulis et Folium Brassicae capitatae etc.)；
Nutritional solution: home gardening concentrated nutrient solution (is executed and can be obtained gardening product company)；
Box for feeding pests: 45 × 45 × 45cm；
Culture vessel: high 10-15cm, the glass container of bore 6-10cm；
Dactylethrae: diameter 1.5cm, high 6cm
RXZ-380B type intelligence growth cabinet (south of the River, Ningbo Instrument Ltd.)；
NAA (naphthalene acetic acid 1-Naphthaleneacetic acid, Shaanxi Sen Lang biochemical industry company limited)；
Potassium permanganate (Potassium Permanganate, Zhengzhou Di Ke chemical products company limited).
1.2 method
1.2.1 the cultivation of host plant
The preparation of auxin: accurately weigh the former medicine of NAA, the ethanol with 95%, as solvent, is made into 1mg/ml mother solution standby.Take
Mother solution is diluted with distilled water into the auxin solution of 1000ppm.
The cutting and sterilizing of water planting material: choose robust growth, the Caulis et Folium Brassicae capitatae that balling is smooth, first divest outer residual leaf 2-3 sheet,
With 1/3rd of scraper crosscutting removing leaf-head top, then cut base section rhizome with cutter, expose fresh rhizome tissue, stem
Bottom notches, after alcohol-pickled 1 minute of volume fraction 75%, take out and put in aseptic cup, high by mass concentration 0.1%
Potassium manganate aqueous solution processes 10 minutes, then with after aseptic water washing otch 2-3 time, then the bottom notches of stem is stained with the life prepared
Long cellulose solution 30 seconds, taking-up is dried.After processing, leaf-head puts into culture vessel (the high 10-15cm, bore 6-added with distilled water
In 10cm), make the bottom notches of stem, go deep into water surface 0.5-1cm, can be used as host plant for rearing standby.Water planting Caulis et Folium Brassicae capitatae processes, raises
The procedure chart of diamondback moth is as shown in Figure 1.
1.2.2 the impact that water planting Caulis et Folium Brassicae capitatae is taken root by different temperatures
Temperature sets 4 process, respectively 16,20,24,28 DEG C, often processes 4 times and repeats, relative humidity 70%, photoperiod L:
D=16: 8, intensity of illumination 4000lx), change 1 water every 3d, after starting long root, change water every time, add nutritional solution 1:1000 (battalion
Nutrient solution stock solution obtains with the preparation of distilled water volume ratio 1:1000), carry out observing and recording the upgrowth situation of root every day.Record each
Process time of starting to take root, root stops growing the time and side root starts growth time, and root stops growing measurement root length, records side
Radical.
1.2.3 the impact that Caulis et Folium Brassicae capitatae is taken root by different substrates
Substrate sets 3 process, respectively tap water, containing saturation water (saturation water, it is simply that have the moisture content of abundance, occur without again
Open fire) Vermiculitum and containing the perlite of saturation water, often process 4 times and repeat, in temperature 24 DEG C, relative humidity 70%, photoperiod L: D
=16: 8, compare, under the conditions of intensity of illumination 4000lx, the impact that Caulis et Folium Brassicae capitatae is taken root by different substrates, the same 1.2.2 of condition of culture.
1.2.4 the different method for breeding impacts on diamondback moth breeding potential
If 3 process, water planting Caulis et Folium Brassicae capitatae prepared by the most potted plant Caulis et Folium Brassicae capitatae, Vermiculitum Turnip Sprouts and step 1.2.1, often process 4 times
Repeat.Potted plant Caulis et Folium Brassicae capitatae and Turnip Sprouts are respectively according to (the 1981) " Turnip Sprouts such as (2001) such as Chen Zongqi " the white cultivating method of Flos Nelumbinis ", Liu Chuanxiu
Vermiculitum method " prepare.
For examination diamondback moth be indoor many generations raise population, 100 pupas of the diamondback moth collected be put into case of laying eggs (45 ×
45 × 45cm), cultivate under conditions of (temperature 24 ± 1 DEG C, RH 70%-75%, L:D=16:8) and sprout wings, more than 50% adult
After emergence, different host plants is put in different egg case 24h of laying eggs respectively, takes out, meet the host after ovum in temperature 24
DEG C, raise to pupating under the conditions of relative humidity 70%, photoperiod L: D=16: 8, intensity of illumination 4000lx.Weigh 20 pupa weights,
And the pupa after different hosts being raised is respectively put in case of laying eggs cultivation and sprouts wings, and cultivates under the same conditions as above, sprouts wings
After, recording eclosion rate, and the adult pairing that the same day is sprouted wings is placed in dactylethrae (diameter 1.5cm, high 6cm), the mouth of pipe is the most cotton
Flower, putting in pipe to inhale has the cotton ball of 15% hydromel, and it is 50 right to join altogether, records ovum amount, the breeding number such as the incubation rate of ovum, eclosion rate
According to.
1.2.5 data calculate
Significance of difference analysis is carried out by SPSS Duncan method.
2 results and water planting Caulis et Folium Brassicae capitatae root growth situation under analysis 2.1 different disposal
Fig. 2 is the comparison diagram taken root after water planting Caulis et Folium Brassicae capitatae root growth 20d under different temperatures.Table 1 is that treatment of different temperature is sweet
The statistical data of blue root volume, from table 1 and Fig. 2 it can be seen that 16 DEG C of process, Caulis et Folium Brassicae capitatae rooting rate is slow, and number of taking root
Measuring less, root length gesture is the most bad, and after processing 30d, the increment of root is little, the new root of induction does not has side root produce；At 20 DEG C
The lower rooting rate of reason is relatively slow, and after processing 30d, quantity of taking root is many, but the increment of root is little, root length 36.20mm, the new root of induction
On have side root to produce；24,28 DEG C process lower rooting rate soon, and quantity of taking root is more, and after processing 30d, the increment of root is big, root
On the upside of 80mm, the new root of induction, root is vigorous, and 24 DEG C of times starting to take root are the 7th day, and required time of taking root is 20d, and 28
DEG C time starting to take root is the 6th day, and required time of taking root is 12d.From Fig. 2 and Biao 1 it can be seen that 24 DEG C and 28 DEG C of water lure sweet
After blue rooting treatment 30d, root system is the longest, and lateral root growth is vigorous, and 15 DEG C, 20 DEG C process between significant difference.
Table 2 processes the statistical data of Caulis et Folium Brassicae capitatae root volume for different substrates, from table 2 it can be seen that the condition of 24 DEG C
Under, the increment of Caulis et Folium Brassicae capitatae rooting rate, root, as the substrate of water planting Caulis et Folium Brassicae capitatae, is had aobvious by water, Vermiculitum and 3 kinds of different materials of perlite
The impact write, pure broken adopts that to use water as the substrate of water planting Caulis et Folium Brassicae capitatae better.
Table 1 treatment of different temperature Caulis et Folium Brassicae capitatae root volume
Note: lower case differs expression significant difference (P=0.05).
Root length in table 1 is average root length.
Table 2 different substrates processes Caulis et Folium Brassicae capitatae root volume
Note: lower case differs expression significant difference (P=0.05).
Root length in table 2 is average root length.
The 2.2 different method for breeding impacts on diamondback moth breeding potential
Diamondback moth instance graph is raised as shown in Figure 3 with different method for breeding.Table 3 is that diamondback moth is bred by different method for breeding
Rate affect tables of data, from the results shown in Table 3, diamondback moth and potted plant Caulis et Folium Brassicae capitatae, the Vermiculitum Turnip Sprouts raised with water planting Caulis et Folium Brassicae capitatae
The diamondback moth that method is raised difference on pupa weight, eclosion rate, single female egg laying amount, incubation rate is all notable, and indoor water planting Caulis et Folium Brassicae capitatae is described
It is feasible that Seedling raises diamondback moth, is to ensure that the normal development of diamondback moth, breeding, and effect to be better than other several
The process of condition.And water planting Caulis et Folium Brassicae capitatae of the present invention makes simple, low cost, the cultivation phase is short, and feedstuff is supplied at any time, facilitates indoor
The mass rearing of diamondback moth.With common potted plant cultivate compared with saving of labor, save time, cultivation the phase short, cultivate low cost, be not bullied
The conditions such as time, soil limit, more convenient.
The different method for breeding impact on diamondback moth breeding potential of table 3
Note: lower case differs expression significant difference (P=0.05).
Claims (3)
1. the method with water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth, it is characterised in that said method comprising the steps of:
(1) host plant is cultivated
Choose the Caulis et Folium Brassicae capitatae that robust growth, balling are smooth, divest residual leaf, 1/3rd of crosscutting removing leaf-head top, then cut the end
The part rhizome in portion, exposes fresh rhizome tissue, and the bottom notches of the stem ethanol water of volume fraction 75% is soaked 1
After minute, take out and put in aseptic cup, process 10 minutes with mass fraction 0.1% potassium permanganate solution, then rush with sterilized water
After washing 2-3 time, then the bottom notches of stem being stained with auxin solution 30 seconds, taking-up is dried, the leaf-head after being processed；Described growth
Cellulose solution is prepared by the following method: naphthalene acetic acid is dissolved in the ethanol of volume fraction 95% preparation and obtains the mother of 1mg/ml concentration
Liquid, then by mother solution with distilled water diluting to concentration 1000ppm；
Leaf-head after processing is put in the culture vessel equipped with water or the substrate containing saturation water, makes the bottom notches of stem go deep into water
0.5-1cm under face, gained water planting Caulis et Folium Brassicae capitatae i.e. can be used for diamondback moth and raises；Described substrate is Vermiculitum or perlite；
(2) diamondback moth is raised
Diamondback moth 50～150 pupas are put into case of laying eggs, at temperature 24 ± 1 DEG C, relative humidity 70-75%, photoperiod L:D=
Sprout wings under conditions of 16:8, after more than 50% adult eclosion, the water planting Caulis et Folium Brassicae capitatae of step (1) put into 24h of laying eggs in case of laying eggs,
Take out, connect the water planting Caulis et Folium Brassicae capitatae after ovum growth cabinet in, temperature 24 DEG C, relative humidity 70%, photoperiod L: D=16:
8, diamondback moth is raised under conditions of intensity of illumination 4000lx to pupating.
2. the method for claim 1, it is characterised in that in described step (1), the leaf-head after processing puts into dress water
In culture vessel, making the bottom notches of stem go deep into underwater 0.5-1cm, gained water planting Caulis et Folium Brassicae capitatae is raised for diamondback moth.
3. the method for claim 1, it is characterised in that the repeatable operating procedure of the diamondback moth (2) after pupating, is laying eggs
Cultivating in case to more than 50% adult eclosion, place into fresh water planting Caulis et Folium Brassicae capitatae and carry out laying eggs, raising, each water planting Caulis et Folium Brassicae capitatae can
Raising for 200-300 one complete generation of head diamondback moth.
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