CN106106375B - A method of with mass rearing diamondback moth in water planting wild cabbage room - Google Patents
A method of with mass rearing diamondback moth in water planting wild cabbage room Download PDFInfo
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- CN106106375B CN106106375B CN201610505668.0A CN201610505668A CN106106375B CN 106106375 B CN106106375 B CN 106106375B CN 201610505668 A CN201610505668 A CN 201610505668A CN 106106375 B CN106106375 B CN 106106375B
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- diamondback moth
- wild cabbage
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- water planting
- raising
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The present invention provides a kind of methods with water planting wild cabbage raising diamondback moth, under diamondback moth rearing conditions, 24 DEG C of temperature, relative humidity 70%, photoperiod L: D=16: 8, water planting is carried out to commodity wild cabbage, it takes root good, single plant water planting wild cabbage can be used for the raising of 200-300 diamondback moths one complete generation, and the diamondback moth that each Biological indicators of the diamondback moth of raising are raised with potting wild cabbage, vermiculite Turnip Sprouts method is not significantly different.This method is easy to operate, at low cost, and feed is supplied at any time, keeps the mass rearing of indoor diamondback moth more convenient.
Description
One, technical field
The present invention relates to a kind of methods with mass rearing diamondback moth in water planting wild cabbage room.
Two, background technique
Diamondback moth Plutella xylostella (L.) belongs to Lepidoptera, plutellidae, alias: small blueness worm, pointed at both ends, hang spring
Worm, 40 kinds of crop in cruciferae such as main harm wild cabbage, broccoli, leaf mustard, cauliflower, Chinese cabbage, rape, radish, are worldwide ten
One of important pests of Zi Hua section vegetables (Feng Xia etc., 2011) have the ability (Xing Kun etc., 2015) migrated at a distance.Pickles
The features such as moth distribution is wide, and reproductive capacity is high, and life cycle is short, generation overlap, development of drug resistance fast (Liang Pei etc., 2001), becomes elder brother
The important research object in the fields such as worm drug resistance, physiology, pathology, toxicology (Yang Fengshan, 2004) is that laboratory insect is ground
Study carefully, the standard worm kind (Lin Kun, 2011) of biological pesticide toxicity evaluation test.
The indoors artificial raising of diamondback moth Plutella xylostella L is the basis for carrying out the research of diamondback moth items.
Currently, the common method of diamondback moth laboratory rearing has Ke Lidao and square chrysanthemum lotus (1981) with germination vegetable seed method, Liu Chuanxiu etc.
(1981) Turnip Sprouts vermiculite method is used, both methods raised with seedling, feed is easy to obtain, but the quantity of food that seedling provides has
Limit, it is difficult to mass rearing, and in diamondback moth larvae growth and development process, often, part nymph cannot shift for switching, worm amount damage
It loses greatly, and is easy to catch an illness, the inconvenient high-volume of pupa is collected and saved after pupating.Chen Zongqi etc. (2001) is using lotus as pickles
The feed of moth, Yang Fengshan etc. (2004) raise diamondback moth with cabbage seedling, can largely be developed neat selected insect source, but post
The main plant culture phase is long, endangers vulnerable to worms such as other pests such as cabbage caterpillar, aphid, difficult management, climate, soil, place etc.
The limitation of condition, feed cannot be supplied in time, cultivate at high cost.Manually feed is raised by Xu Qin (2005), Mo Meihua (2007)
Diamondback moth needs special formula, operates more complicated, and general laboratory, which uses, certain limitation.Lin Kun etc.
(2011) green vegetables Turnip Sprouts method is used, diamondback moth lays eggs on green vegetables Turnip Sprouts, it is raised after egg hatching with the cabbage leaves bought back in the market,
It needs 2d to replace 1 time, guarantees that the freshness of dish leaf is difficult, frequent to transfer, it is unfavorable to grow to diamondback moth, and easily susceptible.
Three, summary of the invention
In order to solve the problems, such as to raise the various of diamondback moth in the prior art, the present invention is comprehensive on the basis of forefathers study
Above-mentioned method for breeding is explored using the wild cabbage bought back in the market, after carrying out a series of processing to it, for diamondback moth raising
Method.Herein a kind of method for breeding of low cost simple will be provided for indoor a large amount of continuously rearing diamondback moths.
Specifically, the technical solution adopted by the present invention is that:
A method of with mass rearing diamondback moth in water planting wild cabbage room, the described method comprises the following steps:
(1) host plant is cultivated
The smooth wild cabbage of robust growth, balling is chosen, strips residual leaf, the crosscutting one third for removing leaf-head top, then cut
The part rhizome for going to bottom, exposes fresh rhizome tissue, by the bottom notches of the stem ethanol water of volume fraction 75%
After impregnating 1 minute, taking-up is put into sterile cup, is handled rhizome notch 10 minutes with 0.1% potassium permanganate solution of mass fraction,
Again with after aseptic water washing 2-3 times, then the bottom notches of stem are stained with auxin solution 30 seconds, taking-up is dried, and obtains that treated
Leaf-head；The auxin solution is prepared by the following method: methyl α-naphthyl acetate being dissolved in prepare in the ethyl alcohol of volume fraction 95% obtains
Then mother liquor is diluted to concentration 1000ppm with distilled water by the mother liquor of 1mg/ml concentration；
By treated, leaf-head is put into the culture vessel of the matrix equipped with water or containing saturated water, keeps the bottom notches of stem deep
0.5-1cm under upstream face, gained water planting wild cabbage can be used to diamondback moth raising；The matrix is vermiculite or perlite.
(2) diamondback moth is raised
50~150 pupas of diamondback moth (preferably 100 pupas) are put into oviposition case, at 24 ± 1 DEG C of temperature, relative humidity
It cultivates and sprouts wings under conditions of 70%, photoperiod L:D=18:8, after 50% or more adult eclosion, the water planting wild cabbage of step (1)
It being put into oviposition case and lays eggs for 24 hours, take out, the water planting wild cabbage after connecing ovum is in growth cabinet, at 24 DEG C of temperature, relative humidity
70%, diamondback moth is raised under conditions of photoperiod L: D=16: 8, intensity of illumination 4000lx to pupating.
Diamondback moth after pupating repeats operating procedure (2), in oviposition case after culture to 50% or more adult eclosion, then
It is put into fresh water planting wild cabbage to be laid eggs, raised, each water planting wild cabbage can be used for 200-300 diamondback moths one complete generation
Raising.
In the step (1), preferably by treated, leaf-head is put into the culture vessel of dress water, keeps the bottom notches of stem deep
0.5-1cm under upstream face, gained water planting wild cabbage are raised for diamondback moth.Directly it is better than matrix culture with Aquaponic effect.
Wild cabbage used in the present invention (Brassica oleracea L.) can directly be bought in market and obtain, generally tie
Cabbage is also known as cabbage, cabbage, cabbage etc..
In the step (1), 1 water was preferably changed every 3 days, further, after water planting wild cabbage starts long root, changed water every time
When nutrient solution is added, obtained by nutrient solution stoste and water according to volume ratio 1:1000 mixed preparing.The nutrient solution stoste can be straight
It connects purchase to obtain, it is the garden Shi Ke get used in the embodiment of the present invention that all kinds of plants or fruit vegetable nutrient liquid, which are used equally for the present invention,
The home gardening concentrated nutrient solution of skill Products Co., Ltd production.
The present invention obtains water planting wild cabbage and is used for using the wild cabbage bought back in the market after carrying out a series of processing cultures to it
Diamondback moth raising is raised, can obtain and preferably raise the pickles chrysalis weight 7.15mg as a result, raising, the eclosion rate 91.85% of pupa,
Single female oviposition 86.70, the hatching rate 94.32% of ovum, the diamondback moth raised with potting wild cabbage, vermiculite Turnip Sprouts method has significance difference
It is different.This method is easy to operate, at low cost, and the cultivation phase is short, and feed is supplied at any time, facilitates the mass rearing of indoor diamondback moth.
The method of the present invention is low in cost, and operation is simple, can guarantee the normal development and breeding of diamondback moth, with common potting
Compared to saving of labor, time saving, the cultivation phase is short for plant culture, cultivates at low cost, and the conditions such as climate, soil do not limit, more convenient.
Four, Detailed description of the invention
The processing of Fig. 1 water planting wild cabbage, the procedure chart for raising diamondback moth, from left to right, respectively untreated wild cabbage, excision leaf
The one third on ball top and the wild cabbage of part rhizome, cultivate at a temperature of 24 DEG C the wild cabbage taken root 10 days, raising diamondback moth it is sweet
It is blue.
The comparison diagram that water planting wild cabbage root growth is taken root after 20 days under Fig. 2 different temperatures.
Fig. 3 raises diamondback moth instance graph with different method for breeding.
Five, specific embodiment
Technical solution of the present invention is described further with specific embodiment below, but protection scope of the present invention is not
It is limited to this.
Embodiment 1
1 materials and methods
1.1 materials and equipment
Wild cabbage (Brassica oleracea L.), buy marketed cabbage (also known as cabbage, cabbage,
Cabbage etc.)；
Nutrient solution: home gardening concentrated nutrient solution (gardening product company can be obtained by applying)；
Box for feeding pests: 45 × 45 × 45cm；
Culture vessel: high 10-15cm, the glass container of bore 6-10cm；
Dactylethrae: diameter 1.5cm, high 6cm
RXZ-380B type intelligence growth cabinet (Ningbo Jiangnan Instrument Ltd.)；
NAA (methyl α-naphthyl acetate 1-Naphthaleneacetic acid, Shaanxi Sen Lang biochemical industry Co., Ltd)；
Potassium permanganate (Potassium Permanganate, Zhengzhou Di Ke chemical products Co., Ltd).
1.2 method
1.2.1 the culture of host plant
The preparation of auxin: accurately weighing NAA raw medicine, and using 95% alcohol as solvent, it is spare to be made into 1mg/ml mother liquor.It takes
Mother liquor is diluted with distilled water into the auxin solution of 1000ppm.
Water planting material cutting and sterilizing: robust growth is chosen, the smooth wild cabbage of balling strips outer residual leaf 2-3 piece first,
With the crosscutting one third for removing leaf-head top of scraper, then with knife bottom part rhizome is cut, exposes fresh rhizome tissue, stem
Bottom notches, after being impregnated 1 minute with the alcohol of volume fraction 75%, taking-up is put into sterile cup, high with mass concentration 0.1%
Mangaic acid aqueous solutions of potassium is handled 10 minutes, then with after aseptic water washing notch 2-3 times, then the bottom notches of stem are stained with prepared life
Long element solution 30 seconds, taking-up is dried.Leaf-head after processing is put into culture vessel (the high 10-15cm, bore 6- added with distilled water
In 10cm), make the bottom notches of stem, gos deep into water surface 0.5-1cm, it is spare to can be used as host plant for rearing.The processing of water planting wild cabbage, raising
The procedure chart of diamondback moth is as shown in Figure 1.
1.2.2 the influence that different temperatures takes root to water planting wild cabbage
Temperature sets 4 processing, and respectively 16,20,24,28 DEG C, 4 repetitions of every processing, relative humidity 70%, photoperiod L:
D=16: 8, intensity of illumination 4000lx), 1 water is changed every 3d, after starting long root, changes water every time, nutrient solution 1:1000 (battalion is added
Nutrient solution stoste and distilled water volume ratio 1:1000 prepare to obtain), the upgrowth situation for observing and recording root is carried out daily.Record is each
Time that processing starts to take root, root stop growing the time and lateral root starts growth time, and root, which stops growing, measures root long, records side
Radical.
1.2.3 the influence that different substrates take root to wild cabbage
Matrix sets 3 processing, respectively tap water, containing saturated water, (saturated water is exactly sufficient moisture content, and does not occur
Bright water) vermiculite and perlite containing saturated water, 4 repetitions of every processing, in 24 DEG C of temperature, relative humidity 70%, photoperiod L: D
=16: 8, compare the influence that different substrates take root to wild cabbage, the same 1.2.2 of condition of culture under the conditions of intensity of illumination 4000lx.
1.2.4 influence of the different method for breeding to diamondback moth breeding potential
If the water planting wild cabbage of 3 processing, respectively potting wild cabbage, vermiculite Turnip Sprouts and step 1.2.1 preparation, every processing 4 times
It repeats.Potting wild cabbage and Turnip Sprouts are respectively according to (1981) " Turnip Sprouts such as Chen Zongqi etc. (2001) " lotus cultivating method ", Liu Chuanxiu
Vermiculite method " prepares.
The population of indoor mostly generation raising for examination diamondback moth, 100 pupas of the diamondback moth being collected into be put into oviposition case (45 ×
45 × 45cm), it cultivates and sprouts wings under conditions of (24 ± 1 DEG C of temperature, RH 70%-75%, L:D=16:8), 50% or more adult
After emergence, different host plants is put into respectively in different egg case and is laid eggs for 24 hours, is taken out, the host after connecing ovum is in temperature 24
DEG C, it is raised under the conditions of relative humidity 70%, photoperiod L: D=16: 8, intensity of illumination 4000lx to pupating.20 pupa weights are weighed,
And the pupa after different hosts raising is respectively put into cultivate in oviposition case and is sprouted wings, cultivates, sprouts wings under the same conditions as above
Afterwards, eclosion rate is recorded, and the adult pairing that the same day sprouts wings is placed in dactylethrae (diameter 1.5cm, high 6cm), nozzle cotton beyond the Great Wall
It spends, the cotton ball inhaled and have 15% hydromel is put in pipe, match 50 pairs altogether, record ovum amount, hatching rate, eclosion rate of ovum etc. breed number
According to.
1.2.5 data calculate
Significance difference analysis is carried out with SPSS Duncan method.
Water planting wild cabbage root growth situation under 2.1 different disposals of 2 results and analysis
Fig. 2 is the comparison diagram taken root after water planting wild cabbage root growth 20d under different temperatures.Table 1 is that treatment of different temperature is sweet
The statistical data of blue root volume can be seen that under 16 DEG C of processing from table 1 and Fig. 2, and wild cabbage rooting rate is slow, and number of taking root
Measure less, root long gesture is also bad, and after handling 30d, the increment of root is little, does not have lateral root generation in the new root of induction；At 20 DEG C
It is slower to manage lower rooting rate, after processing 30d, quantity of taking root is more, but the increment of root is little, root long 36.20mm, the new root of induction
On have lateral root generation；24, the lower rooting rate of 28 DEG C of processing is fast, and quantity of taking root is more, and after handling 30d, the increment of root is big, root
Vigorous up to lateral root on 80mm, the new root of induction, 24 DEG C of times for starting to take root are the 7th day, are 20d the time required to taking root, 28
DEG C time for starting to take root is the 6th day, is 12d the time required to taking root.From Fig. 2 and table 1 as can be seen that 24 DEG C and 28 DEG C of water lure it is sweet
After blue rooting treatment 30d, root system longest, and lateral root growth is vigorous, the significant difference between 15 DEG C, 20 DEG C of processing.
Table 2 is the statistical data that different substrates handle wild cabbage root volume, from table 2 it can be seen that in 24 DEG C of condition
Under, the matrix of 3 kinds of water, vermiculite and perlite different materials as water planting wild cabbage has the increment of wild cabbage rooting rate, root aobvious
The influence of work, the pure broken matrix better effect using water as water planting wild cabbage.
1 treatment of different temperature wild cabbage root volume of table
Note: the not identical expression significant difference (P=0.05) of lowercase.
Root long in table 1 is average root long.
2 different substrates of table handle wild cabbage root volume
Note: the not identical expression significant difference (P=0.05) of lowercase.
Root long in table 2 is average root long.
Influence of the 2.2 different method for breeding to diamondback moth breeding potential
It is as shown in Figure 3 with different method for breeding raising diamondback moth instance graph.Table 3 is that different method for breeding breed diamondback moth
The influence tables of data of rate, from the results shown in Table 3, the diamondback moth raised with water planting wild cabbage and potting wild cabbage, vermiculite Turnip Sprouts
The diamondback moth of method raising difference on pupa weight, eclosion rate, single female egg laying amount, hatching rate is significant, illustrates indoor water planting wild cabbage
Seedling raising diamondback moth is feasible, is the normal development that can guarantee diamondback moth, breeding, and to be better than other several for effect
The processing of condition.And water planting wild cabbage production of the present invention is simple, and at low cost, the cultivation phase is short, and feed is supplied at any time, facilitates interior
The mass rearing of diamondback moth.With the culture of common potted plant compared to saving of labor, time saving, the cultivation phase is short, and cultivation is at low cost, is not bullied
The limitation of the conditions such as time, soil, it is more convenient.
Influence of the different method for breeding of table 3 to diamondback moth breeding potential
Note: the not identical expression significant difference (P=0.05) of lowercase.
Claims (3)
1. a kind of method with mass rearing diamondback moth in water planting wild cabbage room, it is characterised in that the described method comprises the following steps:
(1) host plant is cultivated
The smooth wild cabbage of robust growth, balling is chosen, strips residual leaf, the crosscutting one third for removing leaf-head top, then cut bottom
The part rhizome in portion, exposes fresh rhizome tissue, and the ethanol water of the bottom notches of stem volume fraction 75% is impregnated 1
After minute, taking-up is put into sterile cup, is handled 10 minutes with 0.1% potassium permanganate solution of mass fraction, then rushed with sterile water
After washing 2-3 times, then the bottom notches of stem are stained with auxin solution 30 seconds, taking-up is dried, the leaf-head that obtains that treated；The growth
Plain solution is prepared by the following method: methyl α-naphthyl acetate being dissolved in preparation in the ethyl alcohol of volume fraction 95% and obtains the mother of 1mg/ml concentration
Then mother liquor is diluted to concentration 1000ppm with distilled water by liquid；
By treated, leaf-head is put into the culture vessel of the matrix equipped with water or containing saturated water, and the bottom notches of stem is made to go deep into water
0.5-1cm under face, gained water planting wild cabbage can be used to diamondback moth raising；The matrix is vermiculite or perlite；
(2) diamondback moth is raised
50~150 pupas of diamondback moth are put into oviposition case, at 24 ± 1 DEG C of temperature, relative humidity 70-75%, photoperiod L:D=
Sprout wings under conditions of 16:8, after 50% or more adult eclosion, the water planting wild cabbage of step (1) be put into oviposition case and is laid eggs for 24 hours,
Take out, water planting wild cabbage after connecing ovum growth cabinet in, at 24 DEG C of temperature, relative humidity 70%, photoperiod L: D=16:
8, under conditions of intensity of illumination 4000lx middle raising diamondback moth to pupating.
2. as described in claim 1 with the method for mass rearing diamondback moth in water planting wild cabbage room, it is characterised in that the step
(1) in, by treated, leaf-head is put into the culture vessel of dress water, and the bottom notches of stem is made to go deep into underwater 0.5-1cm, gained
Water planting wild cabbage is raised for diamondback moth.
3. as described in claim 1 with the method for mass rearing diamondback moth in water planting wild cabbage room, it is characterised in that after pupating
Diamondback moth repeats operating procedure (2), and in oviposition case after culture to 50% or more adult eclosion, it is sweet to place into fresh water planting
Indigo plant is laid eggs, is raised, and each water planting wild cabbage can be used for the raising of 200-300 diamondback moths one complete generation.
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| CN106962293B (en) * | 2017-04-05 | 2020-07-10 | 山西省农业科学院植物保护研究所 | Indoor breeding method for single-head plutella xylostella |
| CN107279073B (en) * | 2017-08-17 | 2021-04-23 | 广州市林业和园林科学研究院 | Indoor breeding method for coconut leaf moths |
| CN108207822B (en) * | 2018-03-12 | 2020-10-02 | 内蒙古农业大学 | Artificial mass rapid breeding technical method for Aphidius fusceolatus |
| CN109197786B (en) * | 2018-10-31 | 2021-10-29 | 山西农业大学 | Method for breeding large amount of semi-closed curvicauda curvularia indoors |
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