CN109874448B - Method for promoting pea seed germination and seedling growth by using plant leaching liquor - Google Patents
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Abstract
The invention discloses a method for promoting pea seed germination and seedling growth by using plant leaching liquor, and relates to the technical field of agriculture. The invention comprises the following steps: preparing an SS01 experimental instrument; preparing SS02 pea seeds; preparing SS03 willow branch extract; SS04 experiments; culturing SS05 seeds; recording of SS06 experimental data; processing SS07 experimental data; the results of the SS08 experiment are summarized. According to the invention, willow branches of different varieties are prepared into willow branch leaching solutions, the influence of the willow branch leaching solutions with different concentrations on seed germination and seedling growth of peas is tested, the influence of the willow branch leaching solutions on pea seed germination can be obtained by comparing the seed germination rate and the germination index, and the influence of the willow branch leaching solutions on pea seedling growth can be obtained by comparing the seedling activity index, the seedling root length, the seedling stem length and the number of lateral roots, so that the problem that the influence of the willow branch leaching solutions on pea growth cannot be accurately obtained by the existing test method is solved, and technical support is provided for pollution-free agriculture and organic pea seedling vegetable production.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a method for promoting pea seed germination and seedling growth by using plant leaching liquor.
Background
Peas have strong adaptability, are cultivated in various places, have more fertilizer requirements, and scientific fertilization is an effective measure for increasing the yield of peas, but the large use of chemical fertilizers causes great harm to human bodies and land environments, nitric acid substances in the chemical fertilizers can be reduced into nitrite by human bacteria, the nitrite is a carcinogenic substance, and the long-term large-scale application of the chemical fertilizers can cause soil acidification in a short period, particularly when single-variety chemical fertilizers are continuously applied, the toxic substances can be released or the toxicity of the toxic substances is enhanced, so that the harmful effects on the biological production are caused, and therefore, the research of green and harmless regulators becomes necessary.
Willow has fast germination, various species and strong environmental adaptability, willow branches contain a plurality of growth promoting substances, such as salicylic acid, which is a phenolic substance and has important physiological functions in the aspects of plant growth, development, maturity, aging regulation and stress resistance induction.
At present, the germination of pea seeds and the growth of seedlings by using willow branch leaching liquor are not researched, accurate data can be obtained only by carrying out a comparison experiment in order to ensure that the willow branch leaching liquor has a promotion effect on the growth of peas, and meanwhile, an experiment is needed to determine which concentration of the willow branch leaching liquor has a promotion or inhibition effect on the growth of peas, so that the experiment of the willow branch leaching liquor on the germination of pea seeds and the growth of seedlings is a technical problem to be solved by technical personnel in the field.
Disclosure of Invention
The invention aims to provide a method for promoting pea seed germination and seedling growth by applying plant leaching liquor, willow branch leaching liquor is prepared from willow branches of different varieties, experiments are carried out on the influence of the willow branch leaching liquor with different concentrations on the seed germination and seedling growth of peas, the influence of the willow branch leaching liquor on the pea seed germination can be obtained by comparing the seed germination rate and the germination index, the influence of the willow branch leaching liquor on the pea seedling growth can be obtained by comparing the seedling activity index, the seedling root length, the seedling stem length and the number of lateral roots, and the problem that the influence of the willow branch leaching liquor on the pea growth cannot be accurately obtained by the conventional experimental method is solved.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the invention relates to a method for promoting pea seed germination and seedling growth by using plant leaching liquor, which comprises the following steps:
SS01 laboratory instrument preparation: preparing a high-pressure steam sterilization pot, a clean bench, a blue-mouth bottle, a culture dish and a micro-filter, and sterilizing the clean bench, the blue-mouth bottle, the culture dish and the micro-filter before use;
SS02 pea seed preparation: purchasing fresh pea seeds, selecting new seeds with full seeds, consistent sizes, no plant diseases and insect pests and no damage to embryo parts, rinsing and sterilizing;
preparation of SS03 willow branch extract: collecting different kinds of willow branches, cleaning, performing primary treatment by using NaClO, soaking in sterile water after treatment to obtain a stock solution, sterilizing the stock solution, filtering by using a microfilter, and storing in a blue-mouth bottle;
SS04 experiment: taking out the stock solution to prepare a plurality of leaching solutions with different concentrations, taking out the pea seeds treated in SS02, dividing the pea seeds into a plurality of pea seeds, separating 30 seeds from each pea seed, respectively soaking the pea seeds in the leaching solutions of willows with different concentrations, using distilled water as a control group, removing the leaching solutions after soaking for 24 hours, and washing with distilled water for 3 times;
SS05 seed culture: after water in the seeds is sucked dry by using sterile filter paper, the seeds are placed for 6 hours, the seeds with different concentrations are placed in different culture dishes, leaching liquor or distilled water with different concentrations is soaked on the filter paper, two layers of filter paper are respectively paved below the culture dishes and on the seeds, the condition that the soaking liquor used for soaking the filter paper is the same as the soaking liquor used for the seeds in SS04 is ensured, and the water in the culture dishes is kept during culture;
recording of SS06 experimental data: recording the germination quantity of pea seeds in each culture dish every day, continuously culturing for one week, recording the final germination quantity, and measuring the stem length and the root length of pea seedlings;
processing of SS07 experimental data: calculating the germination rate, germination index, seedling vitality index and allelopathy index according to the data recorded in SS06, and making into a table;
the results of the SS08 experiment are summarized: the influence of willow twig leaching solution on pea seed germination and seedling growth was analyzed by data obtained in SS07 and prepared tables.
Further, the specific data calculation formula in SS07 is as follows:
germination rate: germination rate (germination rate is the percentage of germinated seeds/total experimental seeds) × 100%;
germination rate: the germination rate is (number of seeds normally germinated in seven days/total number of tested seeds) multiplied by 100%;
germination index: germination index ═ (number of seeds germinated at the end of the experiment/total number of days spent in germination experiments) × (number of sprouts at the maximum germination rate in the experiment/number of days to reach this maximum);
seedling vitality index: seedling vigor index ═ germination rate × (seedling root length + seedling stem length);
chemosensory effect index: chemosensory Effect index (RI) 1-C/T;
wherein C is a control value and T is a treatment value;
wherein, RI > 0 is promoting effect, and RI < 0 is inhibiting effect.
Further, the preparation method of the willow branch leaching solution in SS03 comprises the following specific steps:
collecting SS031 willow branches: collecting fresh branches of different types, removing the branches with plant diseases and insect pests, and immediately classifying and refrigerating for later use;
primary treatment with SS 032: taking out the spare willow branches, cutting branches of each variety into small-section branches within the range of 1-2cm, soaking in 3% NaClO for 10-15 minutes, filtering after soaking, and flushing the branches for 3-5 times by using distilled water after filtering until the NaClO on the branches is cleaned;
preparing SS033 stock solution: weighing 0.5kg of branches of different varieties respectively, adding 1L of sterile water respectively, soaking for 24 hours, shaking every 6 hours during soaking, sterilizing the extract by using a sterilizing pot, and filtering the extract by using a fine filter under a super clean bench;
SS034 preservation: and (3) putting the degerming and filtering leaching liquor into a blue-mouth bottle, marking as stock solution, uniformly storing in a refrigerator for preservation, and regulating the temperature of the refrigerator to be 4 ℃ all the time.
Furthermore, the willow branch varieties collected in the SS03 are respectively salix matsudana, salix drooping and salix bambusae.
Further, the concentrations of the different stock solutions in the SS04 were configured to be 25%, 12.5%, 6.25%, and 3.13%, respectively.
Furthermore, the fine filter in the SS033 is a microporous filter, and the aperture of a filter element used in the microporous filter is 0.45um or 0.22 um.
The invention has the following beneficial effects:
according to the invention, the willow branch leaching liquor is prepared from different varieties of willows, the influence of the prepared willow branch leaching liquor on seed germination and seedling growth of peas is tested, the influence of the willow branch leaching liquor on pea seed germination can be obtained by comparing the seed germination rate and the germination index, the influence of the willow branch leaching liquor on pea seedling growth can be obtained by comparing the seedling activity index, the seedling root length, the seedling stem length and the number of lateral roots, the data is obtained accurately, the influence of the willow branch leaching liquor on pea growth can be conveniently analyzed, the willow branch leaching liquor for promoting pea growth can be prepared, and the pea growth efficiency is improved.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a graph comparing germination rates of pea seeds with willow branch leaching solutions of different concentrations and varieties according to the present invention;
FIG. 2 is a graph comparing germination indexes of pea seeds with different concentrations and varieties of willow branch leaching solutions;
FIG. 3 is a comparison graph of willow branch leaching liquor with different concentrations and different varieties on pea seedling vitality indexes;
FIG. 4 is a graph comparing the comprehensive susceptibility indexes of three leaching solutions of Salix matsudana, Salix babylonica and Salix bambusae on peas under four different concentrations.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention relates to a method for promoting pea seed germination and seedling growth by using plant leaching liquor, which comprises the following steps:
SS01 laboratory instrument preparation: preparing a high-pressure steam sterilization pot, a clean bench, a blue-mouth bottle, a culture dish and a micro-filter, and sterilizing the clean bench, the blue-mouth bottle, the culture dish and the micro-filter before use;
SS02 pea seed preparation: purchasing fresh pea seeds, selecting new seeds with full seeds, consistent sizes, no plant diseases and insect pests and no damage to embryo parts, rinsing and sterilizing;
preparation of SS03 willow branch extract: the preparation method specifically comprises the following steps:
collecting SS031 willow branches: collecting fresh branches of three varieties of salix matsudana, salix pendula and salix bambusae, removing pest and disease damage branches, and immediately classifying and refrigerating for later use;
primary treatment with SS 032: taking out the spare willow branches, cutting branches of each variety into small-section branches within the range of 1-2cm, soaking in 3% NaClO for 10-15 minutes, filtering after soaking, and flushing the branches for 3-5 times by using distilled water after filtering until the NaClO on the branches is cleaned;
preparing SS033 stock solution: weighing 0.5kg of branches of different varieties respectively, adding 1L of sterile water respectively, soaking for 24 hours, shaking every 6 hours during soaking, sterilizing the leaching solution by using a sterilizing pot, and filtering the leaching solution by using a fine filter with the aperture of a filter element being 0.45um under a super clean bench;
SS034 preservation: and (3) putting the degerming and filtering leaching liquor into a blue-mouth bottle, marking as stock solution, uniformly storing in a refrigerator for preservation, and regulating the temperature of the refrigerator to be 4 ℃ all the time.
SS04 experiment: taking out the stock solution to prepare four leaching solutions with different concentrations, wherein the concentrations are respectively 25%, 12.5%, 6.25% and 3.13%, taking out the pea seeds treated in SS02, dividing the pea seeds into a plurality of parts of pea seeds, separating 30 grains from each part of pea seeds, respectively soaking the pea seeds in the willow leaching solutions with different concentrations, using distilled water as a control group, removing the leaching solutions after soaking for 24 hours, and washing with distilled water for 3 times;
SS05 seed culture: after water in the seeds is sucked dry by using sterile filter paper, the seeds are placed for 6 hours, the seeds with different concentrations are placed in different culture dishes, leaching liquor or distilled water with different concentrations is soaked on the filter paper, two layers of filter paper are respectively paved below the culture dishes and on the seeds, the condition that the soaking liquor used for soaking the filter paper is the same as the soaking liquor used for the seeds in SS04 is ensured, and the water in the culture dishes is kept during culture;
recording of SS06 experimental data: recording the germination quantity of pea seeds in each culture dish every day, continuously culturing for one week, recording the final germination quantity, and measuring the stem length and the root length of pea seedlings;
processing of SS07 experimental data: calculating the germination rate, germination index, seedling vitality index and allelopathy index according to the data recorded in SS06, and making into a table;
the specific data calculation formula is as follows:
germination rate: germination rate (germination rate is the percentage of germinated seeds/total experimental seeds) × 100%;
germination rate: the germination rate is (number of seeds normally germinated in seven days/total number of tested seeds) multiplied by 100%;
the comparison of germination rates of pea seeds by willow branch leaching solutions with different concentrations and different varieties is shown in figure 1;
germination index: germination index ═ (number of seeds germinated at the end of the experiment/total number of days spent in germination experiments) × (number of sprouts at the maximum germination rate in the experiment/number of days to reach this maximum);
the comparison of germination indexes of pea seeds by willow branch leaching solutions with different concentrations and different varieties is shown in figure 2;
seedling vitality index: seedling vigor index ═ germination rate × (seedling root length + seedling stem length);
the comparison of the willow branch leaching liquor with different concentrations and different varieties on the vitality indexes of the pea seedlings is shown in figure 3;
chemosensory effect index: chemosensory Effect index (RI) 1-C/T;
wherein C is a control value and T is a treatment value;
wherein, when RI is greater than 0, the promoting effect is obtained, and when RI is less than 0, the inhibiting effect is obtained;
the allelopathy effect index table of the willow branch leaching liquor on the germination rate of the pea seeds is as follows:
TABLE 1
The chemosensory effect index table of the willow branch leaching solution on the germination index of the pea seeds is as follows:
TABLE 2
The table of the allelopathy effect indexes of the willow branch leaching solution on the pea seed vitality indexes is as follows:
TABLE 3
The allelopathy effect index of the willow branch leaching solution on the root growth of pea seeds is as follows:
TABLE 4
The allelopathy effect index of the willow branch leaching solution on the stem growth of pea seeds is as follows:
TABLE 5
The allelopathy effect index of the willow branch leaching liquor on the number of lateral roots of pea seeds is as follows:
TABLE 6
The average sensory effect index of 2 indexes of pea seed germination in willow branch leaching liquor is as follows:
| leach liquor | Seed germination rate | Index of germination |
| Salix matsudana (L.) Merr | -0.02027 | -0.03164 |
| Weeping willow | -0.00607 | -0.01326 |
| Bamboo willow | -0.01064 | -0.01869 |
TABLE 7
The average sensory effect indexes of 4 indexes of pea seedlings growing in the willow branch leaching solution are as follows:
| leach liquor | Seedling vitality index | Root length of seedling | Stem length of seedling | Number of lateral roots |
| Salix matsudana (L.) Merr | 0.21899 | 0.20013 | 0.24983 | 0.24874 |
| Weeping willow | 0.13000 | 0.05785 | 0.17987 | 0.23890 |
| Bamboo willow | 0.17466 | 0.12418 | 0.21599 | 0.18039 |
TABLE 8
The proportion of the three leaching solutions of salix matsudana, salix drooping and salix bambusoides in the combined susceptibility index of peas at four different concentrations is shown in fig. 4.
The results of the SS08 experiment are summarized: analyzing the influence of the willow branch leaching solution on pea seed germination and seedling growth through data obtained in SS07 and a prepared table;
1. effect of willow branch extract on pea seed germination
As can be seen from the seed germination rate, the germination index, the seed germination rate and the allelopathy effect index of the pea treated by the three willow branch leaching solutions, the influence of the three willow branch leaching solutions on the seed germination of the pea has both a promoting effect and an inhibiting effect, and the effect is expressed as that the high-concentration inhibition is promoted by low concentration;
for the willow branch leaching liquor for promoting pea seed germination at low concentration, the promotion effect is that the salix matsudana is greater than the bamboo willow and the weeping willow is greater than the weeping willow, for the willow branch leaching liquor for inhibiting pea seed germination at high concentration, the inhibition effect is that the salix matsudana is greater than the bamboo willow and the weeping willow, as can be seen from the average induction effect index of 2 indexes of pea seed germination in the willow branch leaching liquor, the salix matsudana, the weeping willow and the bamboo willow leaching liquor have inhibition effect on pea seed germination, and: germination index is larger than seed germination rate.
2. Effect of willow branch extract on growth of pea seedlings
The seedling activity index, the lateral root number, the seedling activity index, the seedling root length, the seedling stem length and the lateral root number of peas treated by the three willow branch leaching solutions show that the three willow branch leaching solutions all promote the growth of seedlings and have the effects that: 25% is greater than 12.5% is greater than 6.25% is greater than 3.13%, and the growth promotion effect on the pea seedlings can be seen through the average sensory effect index of 4 indexes of the pea seedlings in the willow branch leaching liquor: the salix matsudana is more than the salix bambusae is more than the salix droopingdiana, and the allelopathy effect is as follows: the stem length of the seedling is more than the number of lateral roots, the vitality index of the seedling is more than the root length of the seedling.
3. Comprehensive effect of willow branch leaching liquor on pea germination and seedling growth
As can be seen from the comprehensive sensation indexes of the three leaching liquors of the salix matsudana, the salix pendula and the salix bambusae on the peas under four different concentrations, the comprehensive sensation effects of the three leaching liquors of the willow branches on the peas under the four concentrations are all promotion effects, but the trends of the three leaching liquors of the willow branches are slightly different for different concentrations, and the comprehensive sensation of the leaching liquors of the salix matsudana is as follows: 6.25% > 12.5% > 25% > 3.13%, and for weeping willow and bamboo willow leaching liquor, the comprehensive susceptibility index also decreases along with the decrease of the concentration in four concentration gradients.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (2)
1. The method for promoting pea seed germination and seedling growth by using the plant leaching liquor is characterized by comprising the following steps: the method comprises the following steps:
SS01 laboratory instrument preparation: preparing a high-pressure steam sterilization pot, a clean bench, a blue-mouth bottle, a culture dish and a micro-filter, and sterilizing the clean bench, the blue-mouth bottle, the culture dish and the micro-filter before use;
SS02 pea seed preparation: purchasing fresh pea seeds, selecting new seeds with full seeds, consistent sizes, no plant diseases and insect pests and no damage to embryo parts, rinsing and sterilizing;
preparation of SS03 willow branch extract: collecting different kinds of willow branches, cleaning, performing primary treatment by using NaClO, soaking in sterile water after treatment to obtain a stock solution, sterilizing the stock solution, filtering by using a microfilter, and storing in a blue-mouth bottle;
the willow branch varieties collected in the SS03 are respectively salix matsudana, salix weeping willow and bamboo willow;
the preparation method of the willow branch leaching solution in SS03 comprises the following steps:
collecting SS031 willow branches: collecting fresh branches of different types, removing the branches with plant diseases and insect pests, and immediately classifying and refrigerating for later use;
primary treatment with SS 032: taking out the spare willow branches, cutting branches of each variety into small-section branches within the range of 1-2cm, soaking in 3% NaClO for 10-15 minutes, filtering after soaking, and flushing the branches for 3-5 times by using distilled water after filtering until the NaClO on the branches is cleaned;
preparing SS033 stock solution: weighing 0.5kg of branches of different varieties respectively, adding 1L of sterile water respectively, soaking for 24 hours, shaking every 6 hours during soaking, sterilizing the leaching solution by using a sterilizing pot, and filtering the leaching solution by using a microfilter under an ultra-clean workbench;
SS034 preservation: putting the degerming and filtering leaching liquor into a blue-mouth bottle, marking as stock solution, uniformly storing in a refrigerator for preservation, and regulating the temperature of the refrigerator to be 4 ℃ all the time;
SS04 experiment: taking out the stock solution to prepare a plurality of leaching solutions with different concentrations, taking out the pea seeds treated in SS02, dividing the pea seeds into a plurality of pea seeds, separating 30 seeds from each pea seed, respectively soaking the pea seeds in the willow leaching solutions with different concentrations for 24 hours, removing the leaching solutions, and washing the pea seeds with distilled water for 3 times;
the concentration of the leaching liquor in the SS04 is 6.25% and 3.13%;
SS05 seed culture: after water in the seeds is sucked dry by using sterile filter paper, the seeds are placed for 6 hours, the seeds with different concentrations are placed in different culture dishes, leaching liquor with different concentrations is soaked on the filter paper, two layers of filter paper are respectively paved below the culture dishes and on the seeds, the leaching liquor used by soaking the filter paper is ensured to be the same as that used by the seeds in SS04, and the water in the culture dishes is kept during culture;
recording of SS06 experimental data: recording the germination quantity of pea seeds in each culture dish every day, continuously culturing for one week, recording the final germination quantity, and measuring the stem length and the root length of pea seedlings;
processing of SS07 experimental data: calculating the germination rate, germination index, seedling vitality index and allelopathy index according to the data recorded in SS06, and making into a table;
the results of the SS08 experiment are summarized: the influence of willow twig leaching solution on pea seed germination and seedling growth was analyzed by data obtained in SS07 and prepared tables.
2. The method for promoting germination of pea seeds and growth of seedlings by using plant leaching liquor according to claim 1, wherein the microfilter in SS033 is a microporous filter, and the pore size of the filter element used in the microporous filter is 0.45um or 0.22 um.
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| US4763442A (en) * | 1982-03-17 | 1988-08-16 | University Of Pittsburgh | Plant propagator |
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| CN106167428A (en) * | 2016-08-13 | 2016-11-30 | 李博文 | A kind of bracketplant mill water culture nutrient solution and preparation method thereof |
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| US4763442A (en) * | 1982-03-17 | 1988-08-16 | University Of Pittsburgh | Plant propagator |
| CN101810132A (en) * | 2010-05-11 | 2010-08-25 | 王恩冶 | Planting method of soilless culture pea seedlings |
| CN106167428A (en) * | 2016-08-13 | 2016-11-30 | 李博文 | A kind of bracketplant mill water culture nutrient solution and preparation method thereof |
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