CN110972797A

CN110972797A - Method for improving drought resistance of oil peony by adopting methyl jasmonate

Info

Publication number: CN110972797A
Application number: CN201911387478.3A
Authority: CN
Inventors: 郭丽丽; 侯小改; 王菲; 郭大龙; 魏祯祯; 张红晓
Original assignee: Henan University of Science and Technology
Current assignee: Henan University of Science and Technology
Priority date: 2019-12-27
Filing date: 2019-12-27
Publication date: 2020-04-10
Also published as: CN111903419A

Abstract

The invention discloses a method for improving drought resistance of oil peony by methyl jasmonate, which adopts methyl jasmonate to induce the oil peony to generate drought resistance; the method comprises the following steps: 1) selecting annual oil peony seedlings with consistent growth vigor and no diseases and insect pests for cultivation in 10 months; 2) culturing in greenhouse at room temperature for 5 months next year, and performing normal water management; 3) spraying methyl jasmonate on the oil peony once every 3d for 2 times to obtain the oil peony with drought resistance. The invention adopts methyl jasmonate to induce and treat, and the net photosynthetic rate, transpiration rate and intercellular CO in the leaves₂The concentration, the stomatal conductance, the content of free proline and the content of soluble sugar are increased, the content of malondialdehyde, the content of chlorophyll a, the content of chlorophyll b and the content of carotenoid are decreased, the net photosynthetic rate of the oil peony leaves is obviously improved, the damage of drought stress to peony is reduced, and the improvement of the yield of oil peonyThe capacity of peony to resist drought stress.

Description

Method for improving drought resistance of oil peony by adopting methyl jasmonate

Technical Field

The invention relates to the technical field of peony planting, in particular to a method for improving drought resistance of oil peony by adopting methyl jasmonate.

Background

Peony (Paeonia suffruticosa Andr.) is perennial deciduous shrub of Paeonia in Paeoniaceae, is a traditional famous flower in China, and is called as the king of the flower. The Heluo area has rich peony resources, and is one of the original regions of wild peony in China. In recent years, peony related industries are rapidly developed, and deep-processed products of the peony are widely applied to the fields of appreciation, oil use, eating, medicine and the like. The oil peony is a general name of 'Paeonia ostii' (Paeonia ostii) and 'purpura' (Paeonia rockii) peony with high seed setting rate and high oil yield in peony species, and is a unique emerging woody oil crop in China. The peony seed oil is golden yellow transparent oily liquid, is rich in unsaturated fatty acid (Kim et al, 2014; Mao et al, 2017) which is necessary for human bodies and cannot be synthesized by the human bodies, and the like, and has the content of the unsaturated fatty acid as high as 92 percent, wherein the alpha-linolenic acid accounts for 42 percent and is 40 times of that of the olive oil. The peony seed oil also contains medicinal effective components of peony, and has effects of reducing blood sugar, blood lipid, and cholesterol (Suet al, 2016). 2011 peony seed oil has been approved by the national health committee as a new resource food [2011 No. 9 ]. Oil peonies in their habitat often encounter drought stress caused by insufficient water resources. The method can be used for coping with the ecological conditions of global climate drought change and water resource shortage, realizes the promotion of the novel oil-crop-use peony drought-resistant cultivation technology, and has important significance for realizing agricultural sustainable development.

Various methods are disclosed in the prior art for improving the drought resistance of oil peonies, for example, CN108849476A discloses a breeding method for improving the drought resistance of oil peonies, which combines the traditional hybridization mode with a chemical reagent treatment method, reduces the generation of false positive drought-resistant seeds, reduces the total sample amount of the subsequent hybridization steps, reduces the workload, and improves the breeding efficiency; patent CN106866241A discloses a breeding method for improving drought resistance of oil peony, which combines aging treatment and drought resistance agent to effectively improve germination rate of seeds, improve drought resistance of seeds and improve acre yield of oil peony. Although the prior art achieves the aim of improving the drought resistance of the oil peony, the prior art has the defects of high cost, complex process and long time consumption.

The plant cell membranous substance is degraded under the stress of adverse environment to generate unsaturated fatty acid, and the unsaturated fatty acid generates derivatives of the unsaturated fatty acid such as methyl jasmonate (MeJA) and the like through a series of biological oxidation reactions. MeJA is a plant hormone widely existing in plants, and exogenous spraying of MeJA can stimulate expression of defense plant genes and induce chemical defense reaction of plants.

Therefore, the application of the methyl jasmonate in improving the drought resistance of the oil peonies has important significance in enlarging the growing area of the oil peonies and improving the yield of the oil peonies under the adverse conditions.

Disclosure of Invention

In view of the above, the invention aims to provide a method for improving the drought resistance of oil peonies, aiming at the problem that the oil peonies are susceptible to drought stress during the growth process and influence the normal growth of the oil peonies to cause yield reduction.

In order to achieve the purpose, the invention adopts the following technical scheme:

a method for improving drought resistance of oil peony by adopting methyl jasmonate induces drought resistance of oil peony by adopting methyl jasmonate; the method comprises the following steps:

1) selecting annual oil peony seedlings with consistent growth vigor and no diseases and insect pests for cultivation in 10 months;

2) culturing in greenhouse at room temperature for 5 months next year, and performing normal water management;

3) and (3) spraying methyl jasmonate on the whole bead of the oil peony until the upper and lower surfaces of the leaf drip water, spraying once every 3d, and spraying for 2 times in total to obtain the oil peony with drought resistance.

Preferably, the concentration of the methyl jasmonate sprayed in the step 3) is 50-200 mug/mL.

According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:

1. the invention provides an induction method for promoting the photosynthesis and physiological characteristics of oil peonies under drought and rehydration conditions, which effectively solves the problem that the oil peonies 'paeonia ostii' grow badly under drought stress conditions.

2. The method is simple to operate, exogenous methyl jasmonate (MeJA) with proper concentration is sprayed in the process of the vegetative growth of the oil peony, and the net photosynthetic rate, transpiration rate and intercellular CO in the oil peony leaves are improved₂The concentration, the stomatal conductance, the content of free proline (Pro) and the content of Soluble Sugar (SS) are increased, the content of Malondialdehyde (MDA), chlorophyll a, chlorophyll b and carotenoid is decreased, the net photosynthetic rate of the oil peony leaves is obviously improved, the damage degree of drought stress on paeonia ostii is reduced, and the resistance of paeonia ostii' peony to drought stress is improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 shows the effect of methyl jasmonate of different concentrations on the proline content of peony 'Paeonia ostii' for oil under drought stress;

FIG. 2 is a graph of the effect of methyl jasmonate of different concentrations on the soluble sugar content of oil peony 'paeonia ostii' under drought stress;

FIG. 3 is a graph showing the effect of methyl jasmonate of different concentrations on the MDA content of peony 'Paeonia ostii' for oil under drought stress;

FIG. 4 is a graph showing the effect of methyl jasmonate of different concentrations on the content of photosynthetic pigments of peony tree for oil under drought stress, where a is the change in the content of chlorophyll a; b is the change of chlorophyll b content; c is the change in carotenoid content;

FIG. 5 effect of different concentrations of methyl jasmonate on net photosynthetic rate of oil peony 'Paeonia ostii' under drought stress;

FIG. 6 is a graph of the effect of methyl jasmonate at different concentrations on the transpiration rate of oil peony 'Paeonia ostii' under drought stress;

FIG. 7 is a graph showing the effect of methyl jasmonate of different concentrations on stomatal conductance of oil peony 'Paeonia ostii' under drought stress;

FIG. 8 shows intercellular CO of oil peony 'Paeonia ostii' under drought stress with different concentrations of methyl jasmonate₂The effect of concentration.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The method comprises the following steps: 3-year-old oil-bearing peony 'Paeonia ostii' plants with consistent growth vigor and no plant diseases and insect pests are selected to be planted in plastic flowerpots with the diameter of 20cm and holes at the bottoms, the matrix is farm original soil of Henan university of science and technology, and 1 plant is planted in each pot.

Step two: and moving the seedlings into a greenhouse for maintenance after planting, carrying out normal water management, preventing water shortage and preventing plant diseases and insect pests.

Step three: the whole plant spraying method is adopted, 50 mu mol/L methyl jasmonate is used for induction treatment, and water drops on the upper surface and the lower surface of the leaf are preferably used, and each treatment is set to be 5 times.

Step four: after the methyl jasmonate is treated for 3 days, gradual drought stress treatment is carried out, observation and analysis of phenotypic characteristic change are carried out every day, and the wilting degree of the plants is judged according to the change of the phenotypic characteristics of the paeonia ostii and the drought stress degree of the plants is determined.

Step five: under moderate drought, severe drought and rehydration respectively, determining the change of photosynthetic property of the peony 'paeonia ostii' for oil, simultaneously taking the leaves, quickly freezing the leaves by liquid nitrogen, and storing the leaves at the temperature of minus 80 ℃ for determination of physiological indexes.

Example 2

Step three: the whole plant spraying method is adopted, 100 mu mol/L methyl jasmonate is used for induction treatment, and water drops on the upper surface and the lower surface of the leaf are preferably used, and each treatment is set to be 5 times.

Example 3

Step three: the whole plant spraying method is adopted, and 200 mu mol/L methyl jasmonate is used for induction treatment, preferably water drops on the upper and lower surfaces of the leaves are used, and each treatment is set to be 5 times.

Example 4

Step three: the whole plant spraying method is adopted, and 400 mu mol/L methyl jasmonate is used for induction treatment, preferably water drops on the upper and lower surfaces of the leaves are used, and each treatment is set to be 5 times.

Related experiments:

the test is provided with an absolute control group, a relative control group and a treatment group, wherein the absolute control group is Paeonia ostii ' peony growing in a natural state, the relative control group is Paeonia ostii ' peony sprayed with methyl jasmonate in different concentrations under normal water supply, the treatment group is Paeonia ostii ' peony sprayed with exogenous methyl jasmonate and subjected to gradual drought stress, and the gradual drought stress degree is divided into moderate, severe and rehydration treatment according to the phenotypic change of plants. The photosynthetic property is measured under different drought stress degrees of the peony 'paeonia ostii' for oil respectively, and simultaneously the leaves are taken for measuring the physiological index. The measurement indexes include: proline, MDA, soluble sugars, photosynthetic pigments (chlorophyll a, chlorophyll b, carotenoids), photosynthetic characteristics (net photosynthetic rate, intercellular CO)₂Concentration, porosity conductance, transpiration rate). All indexes were set for 5 replicates, randomly sampled, and the experimental data were statistically analyzed using Excle and SPSS19.0 software and plotted using origine 8.0 software.

As shown in figure 1, the proline content is in a rapid rising trend along with the increase of the stress degree, and the proline content of the control group reaches the highest value under severe drought. After exogenous MeJA is applied, the proline content is increased in different degrees, the growth influence of MeJA induction treatment with different concentrations on the peony 'paeonia ostii' for oil is inconsistent, wherein the proline content of the peony 'plant is obviously increased by 100 mu mol/L MeJA treatment, which indicates that the exogenous MeJA induction treatment can inhibit cell dehydration and improve the drought resistance of the peony'.

As shown in fig. 2, under moderate drought stress conditions, the soluble sugar content rapidly increased, and the drought stress increased the content of osmoregulation substances in the oil peony ' paeonia ostii ' plants, thereby enhancing the water retention capacity of paeonia ostii ' peony leaves. Under drought stress treatment, the content of soluble sugar tends to decrease first and then increase when the concentration of MeJA is 100 mu mol/L. Under the treatment of different drought stress degrees, the soluble sugar content in the leaves of the peony 'paeonia ostii' for oil is lower than that of the corresponding control, and the soluble sugar content is increased after rehydration and is equal to that of the control. Exogenous MeJA treatment can induce the generation of soluble sugar of the permeation regulating substance in the Paeonia ostii Paeonia leaf, thereby improving the drought resistance of Paeonia ostii Paeonia.

As shown in fig. 3, under normal water management conditions, after exogenous MeJA induction treatment, the malondialdehyde MDA content in the peony 'paeonia ostii' leaves for oil has no obvious change and no obvious difference. Under the gradual drought stress treatment, the MDA content is obviously accumulated, and the membrane lipid peroxidation degree is enhanced. After exogenous MeJA is applied, the MDA content in the leaves of the peony 'paeonia ostii' for oil is remarkably reduced under the gradual drought stress treatment, when the exogenous MeJA concentration is 100 mu mol/L, the MDA content in the leaves is the lowest, and is respectively reduced by 96.2% and 68.7% compared with a control; after rehydration treatment, the MDA content in each treatment blade is reduced to some extent under drought stress, and the accumulation of the MDA content is effectively relieved.

As shown in fig. 4, the contents of chlorophyll a, chlorophyll b and carotenoid in the leaves of peony 'paeonia ostii' for oil show a tendency of decreasing first and then increasing with the increase of MeJA concentration under different drought stress levels. After induction treatment with different MeJA concentrations, the chlorophyll a, b and carotenoid contents are lower than those of the contemporary control. Under the treatment of different drought stress degrees, the chlorophyll a content is increased by 4.2 percent and 46.3 percent compared with the CK group supplied with normal water respectively; the chlorophyll b content is increased by 8.2 percent and 58.7 percent; the carotenoid content is increased by 11.8 percent and 43.8 percent. After the induction treatment of exogenous methyl jasmonate, under the treatment of different drought stress degrees, the contents of chlorophyll a, chlorophyll b and carotenoid are all reduced, and when the concentration of MeJA is 100 mu mol/L, the contents are respectively reduced by 21.9%, 20.9%, 37.3% and 27.8% compared with the CK group; the chlorophyll b content is remarkably reduced by 31.5%, 11.0%, 41.3% and 52.1%; the carotenoid content is obviously reduced by 19.0 percent, -1.2 percent, 32.7 percent and 14.5 percent. Therefore, the photosynthetic capacity of the peony 'paeonia ostii' for oil under drought stress can be enhanced by externally applying 100 mu mol/L MeJA.

As shown in FIG. 5, during normal water supply, the photosynthetic capacity of the plant is stronger, and the net photosynthetic rate is higher; during drought stress and rehydration, the net photosynthetic rate is at a lower level, exogenous MeJA treatment has a remarkable promoting effect on 'Paeonia ostii' leaves, and the net photosynthetic rate is improved compared with a control under different concentration treatment; under different degrees of drought stress, when MeJA is 100 mu mol/L, the net photosynthetic rate is increased by 19.7 percent and 11.8 percent compared with the control; after rehydration, the net photosynthetic rate is improved to different degrees by MeJA treatment with different concentrations. Exogenous MeJA treatment can effectively improve the net photosynthetic rate of plants and enhance the photosynthesis intensity of the plants, but cannot restore the plants to the normal CK group level after rehydration.

As shown in fig. 6, under drought stress conditions, the leaf transpiration rate of 'paeonia ostii' showed a tendency of increasing first and then decreasing as the MeJA concentration increases; the transpiration rate at the early stage of drought stress is rapidly and greatly reduced, the transpiration rate is increased in different degrees after the treatment of MeJA, and the treatment effects of various concentrations are different; under different degrees of drought stress and rehydration, the transpiration rate of the leaves of the paeonia ostii treated by 100 mu mol/L MeJA is respectively increased by 16.7%, 28.9% and 214.0% compared with the corresponding CK group.

As shown in fig. 7, exogenous MeJA treatment significantly increased the stomatal conductance of the 'paeonia ostii' leaves, consistent with the trend of change in transpiration rate and net photosynthetic rate. Under severe drought stress and rehydration, the stomatal conductance of the 100 mu mol/L treated Paeonia ostii' leaves is respectively increased by 60.0 percent and 350.0 percent compared with that of a control, and the stomatal conductance reaches an extremely significant level (P < 0.05). After 400 mu mol/L exogenous MeJA treatment, the increase is 44.0 percent and 250.0 percent respectively compared with CK. The effect of the high concentration MeJA treatment on the increase in porosity conductivity is not as significant as the low concentration.

As shown in FIG. 8, exogenous MeJA treatment at different concentrations resulted in intercellular CO in the 'Paeonia ostii' leaves under different treatments₂The concentrations increased to different extents, but the differences were not significant. After rehydration, the effect under 100 μmol/L exogenous MeJA treatment was most significant, increasing by 72.1% compared to the corresponding CK. Intercellular CO of ` Paeonia ostii ` leaves under different drought stress treatment conditions₂The concentration is maintained at a higher level. Thus, the net photosynthetic rate and intercellular CO of the Paeonia ostii' peony plant₂The increase in concentration is inversely related.

In conclusion, exogenous MeJA treatment can enhance the resistance of plants to drought stress, wherein the MeJA treatment effect at 100 mu mol/L is the best.

In the vegetative growth stage of the peony ' paeonia ostii ' for oil, after exogenous MeJA is adopted to induce and treat the paeonia ostii ', the net photosynthetic rate, the transpiration rate and intercellular CO in leaves₂The concentration, porosity conductance, free proline (Pro) content and Soluble Sugar (SS) content all show increasing trend, and Malondialdehyde (MDA) content, chlorophyll a, chlorophyll b and carotenoid content show decreasing trend. Exogenous MeJA induction treatment obviously improves the net photosynthetic rate of the peony leaf for oil, reduces the damage of drought stress to the peony, and improves the drought stress resistance of the peony.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description. The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for improving drought resistance of oil peony by adopting methyl jasmonate is characterized in that the methyl jasmonate is adopted to induce the oil peony to generate drought resistance; the method comprises the following steps:

2. The method for improving the drought resistance of oil peonies by using methyl jasmonate according to claim 1, wherein the concentration of the methyl jasmonate sprayed in the step 3) is 50-200 μ g/mL.