CN105961390B - Application of the methyl jasmonate on plant is waterlogging-resistant - Google Patents

Application of the methyl jasmonate on plant is waterlogging-resistant Download PDF

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Publication number
CN105961390B
CN105961390B CN201610317177.3A CN201610317177A CN105961390B CN 105961390 B CN105961390 B CN 105961390B CN 201610317177 A CN201610317177 A CN 201610317177A CN 105961390 B CN105961390 B CN 105961390B
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methyl jasmonate
waterflooding
application
root system
solution
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CN105961390A (en
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邹学校
欧立军
刘周斌
危革
张竹青
陈文超
戴雄泽
马艳青
杨博智
杨莎
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Tibet Lvzhiyuan Modern Agricultural Technology Co ltd
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INST OF VEGETABLES HUNAN PROV
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Botany (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Cultivation Of Plants (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The present invention's provides a kind of application of methyl jasmonate, and the waterflooding that methyl jasmonate is applied to alleviate shallow root system vegetables injures, and improves its waterlogging-resistant ability.Injury present invention reduces waterflooding to shallow root system vegetables improves its survival rate, has ensured the yield of shallow root system vegetables under flooding condition, has increased peasant income.

Description

Application of the methyl jasmonate on plant is waterlogging-resistant
Technical field
The present invention relates to a kind of application of methyl jasmonate on shallow root system vegetables are waterlogging-resistant, more particularly in Solanaceae capsicum Application on platymiscium is waterlogging-resistant.
Background technology
Capsicum (Capsicum annum L.) Solanaceae capsicum plants, one of maximum vegetable species of China's cultivated area, Per annual planting area up to 1,300,000 hm2 or more, belong to shallow root system plant, root system is thin and delicate, and absorption root is less, when soil moisture is excessive When, the growth of capsicum can be influenced.The harm of In Middle And Lower Reaches of Changjiang River flood stain frequently occurs, and has seriously affected spring and summer and autumn open country is planted The yield and quality of capsicum is trained, and the capsicum 60%-70% of China's plantation is outdoor cropping, damage or crop failure caused by waterlogging has become domestic open country capsicum One of major stress factors of production.Capsicum has stronger drought-resistance ability, but resistance to waterlogging ability is weaker, and capsicum is after by flood, easily Phenomena such as root dead seedling is macerated in appearance, yellowing leaf falls off and fruit drop.Therefore, external measure is taken to improve the energy of resistance to waterlogging of capsicum Power to promote capsicum be widely applied and long season cultivation it is significant.It is existing research shows that external source Ca2+It can pass through Pepper seedling root system internal respiration metabolism is adjusted to alleviate injury of the flooding stress to plant, but it is only capable of a few days ago and flooding in waterflooding Water sprays just effectively one day after, and the less effective after spraying.
In addition, although known methyl jasmonate can improve the vigor of seed under cryopreservation, reply low temperature and salt are improved The ability of the adverse circumstances such as alkali, but not yet there is the application similar with the present invention.
Invention content
The purpose of the present invention is to provide a kind of new applications of methyl jasmonate, methyl jasmonate are applied to alleviate shallow Root system vegetables waterflooding injures, and improves the waterlogging-resistant ability of shallow root system vegetables.
Technical scheme of the present invention
The present invention provides a kind of application of methyl jasmonate, methyl jasmonate is applied to alleviate shallow root system vegetables waterflooding Injury.
It is preferred that the vegetables are Solanaceae capsicum plants.
The particularly preferred vegetables are capsicum.
When the vegetables are capsicum, unexpected alleviation waterflooding injury can be obtained and improve the good of waterlogging-resistant ability Good resultant effect.
The application process of the methyl jasmonate, the shallow root system vegetables foliage-spray jasmonic during waterflooding or after waterflooding Methyl ester solution.
The present invention sprays methyl jasmonate either during waterflooding or after waterflooding, can obtain good alleviation waterflooding The effect of injury.
For example, good effect can also be obtained by spraying within 3-8 days after waterflooding the methyl jasmonate of the present invention.
The present invention includes following preferred technical solution:
In preferred scheme, a concentration of 0.3-2.0mmol L of the methyl jasmonate solution-1
A concentration of 0.6-1.0mmol L of the further preferred methyl jasmonate solution-1
In preferred scheme, methyl jasmonate solution is continuously sprayed 1-12 days.
Further preferably continuously spray methyl jasmonate solution 3-7 days.
The methyl jasmonate solution is preferably the aqueous solution that after being dissolved using ethanol solution plus water obtains.
It is described spray during, preferably continuously spray to shallow root system vegetables blade face to have and be dripped under liquid.
The volumetric concentration of the ethanol solution is preferably 75-95%.
It is preferred that being extractd during waterflooding and to the blade of plant diseases leaf and 50% or more area jaundice after waterflooding, go forward side by side Row loosing soil.
The methyl jasmonate solution is preferably preserved by brown reagent bottle before spraying.
Although the present invention has only done the waterlogging-resistant experiment of capsicum in embodiment, the starting point of research is to improve the anti-of capsicum Flood and alleviation waterflooding injury, it is contemplated that present invention may apply to general vegetables, especially shallow root system is easy by waterflooding The vegetables of injury.
Beneficial effects of the present invention
Although there is some other applications of methyl jasmonate in the prior art, methyl jasmonate can be applied to delay It is that inventor did not expected to solve plant waterflooding injury, also never there is relevant trial in the prior art.
Plant leaf surface during methyl jasmonate is sprayed on waterflooding by inventor by accident or after waterflooding achieves expectation Outer good result.
Injury present invention reduces waterflooding to shallow root system vegetables improves the survival rate of shallow root system vegetables, has ensured and flooded The yield of capsicum, increases peasant income under water condition.
In the waterlogging-resistant experimentation of specific capsicum, inventor has found, especially by the dense of methyl jasmonate solution Advanced optimizing for number of days is spent and sprayed, better plant height, root long, whole strain fresh weight, relative conductivity synthesis can be obtained and changed Kind effect, greatly improves the survival rate of capsicum.
Through a large number of experiments and test, inventor are found surprisingly that in the present invention by spraying methyl jasmonate raising The activities of antioxidant enzymes such as the catalase of capsicum, superoxide dismutase, increase osmotic adjustment proline content, Accumulation and the relative conductivity of malonaldehyde are reduced, while improving the activity of anaerobic respiration relevant enzyme alcohol dehydrogenase, and makes root System keeps higher malic dehydrogenase, is conducive to capsicum and carries out aerobic respiration metabolism.
Description of the drawings
Fig. 1 is to spray various concentration methyl jasmonate to waterflooding capsicum MDA (malonaldehyde) content, Pro (amino acid) content With the influence diagram of CAT (catalase).
Fig. 2 is to spray various concentration methyl jasmonate to waterflooding capsicum SOD (superoxide dismutase) active influence diagram.
Fig. 3 is to spray various concentration methyl jasmonate to waterflooding capsicum ADH (alcohol dehydrogenase) active influence diagram.
Fig. 4 is to spray various concentration methyl jasmonate to waterflooding capsicum MDH (malic dehydrogenase) active influence diagram.
Fig. 5 is to spray different number of days methyl jasmonate to waterflooding capsicum MDA (malonaldehyde) content, Pro (amino acid) content With the influence diagram of CAT (catalase).
Fig. 6 is to spray different number of days methyl jasmonate to waterflooding capsicum SOD (superoxide dismutase) active influence diagram.
Fig. 7 is to spray different number of days methyl jasmonate to waterflooding capsicum ADH (alcohol dehydrogenase) active influence diagram.
Fig. 8 is to spray different number of days methyl jasmonate to waterflooding capsicum MDH (malic dehydrogenase) active influence diagram.
Specific implementation mode
Embodiment 1
A kind of application process of methyl jasmonate, includes the following steps:
The first step, methyl jasmonate are prepared
The jasmonic first of 0.06729g, 0.13458g, 0.2243g, 0.33645g and 0.4486g are weighed by electronic balance Ester pours into the beaker of 1000mL, and the 95% ethyl alcohol dissolving methyl jasmonate of 5mL is added, then 200mL distilled water is used to dilute The volumetric flask constant volume of 1000mL is placed on to 1000mL, it is respectively 0.3mmol L to make its final concentration-1、0.6mmol L-1、 1.0mmol L-1、1.5mmol L-1With 2.0mmol L-1, preserved finally by brown reagent bottle.
Second step, foliage-spray when waterflooding
On the day of waterflooding, the methyl jasmonate of each concentration is placed in watering can, makes its ejection that nebulizes, continuously sprays to capsicum leaf Face continuously sprays 5 days until blade has and dripped under liquid.
Third walks, and manages during waterflooding and after waterflooding
It is extractd during waterflooding and to the blade of pepper diseases leaf and 50% or more area jaundice after waterflooding, and in period Carry out loosing soil.
Table 1 sprays the influence that various concentration methyl jasmonate grows pepper seedling under flooding stress
As it can be seen from table 1 the growing state of pepper seedling can be substantially improved by spraying methyl jasmonate.
Embodiment 2
A kind of application process of methyl jasmonate, includes the following steps:
The first step, methyl jasmonate are prepared
0.2243g methyl jasmonates are weighed by electronic balance, are poured into the beaker of 1000mL, the 95% of 5mL is added Ethyl alcohol dissolves methyl jasmonate, and the volumetric flask constant volume for being then placed on 1000mL with the dilution of 200mL distilled water makes it to 1000mL Final concentration is respectively 1.0mmol L-1, preserved finally by brown reagent bottle.
Second step, foliage-spray when waterflooding
On the day of waterflooding, the methyl jasmonate of each concentration is placed in watering can, makes its ejection that nebulizes, continuously sprays to capsicum leaf Face continuously sprays 1 day, 3 days, 5 days, 7 days, 9 days and 12 days respectively until blade has and dripped under liquid.
Third walks, and manages during waterflooding and after waterflooding
It is extractd during waterflooding and to the blade of pepper diseases leaf and 50% or more area jaundice after waterflooding, and in period Carry out loosing soil.
2 difference of table sprays the influence that number of days methyl jasmonate grows pepper seedling under flooding stress
Processing Plant height [cm] Root long [cm] Whole strain fresh weight [g] Relative conductivity [%]
Normal control 17.40±0.49 10.03±0.33 2.65±0.14 16.13±2.11
Simple submerging treatment 12.67±0.28 4.47±0.15 1.12±0.03 37.20±2.89
1d 12.29±0.15 5.01±0.15 1.32±0.12 36.97±2.91
3d 14.69±0.31 5.89±0.24 1.71±0.15 28..01±2.23
5d 14.57±0.42 6.61±0.37 1.79±0.29 27.99±3.55
7d 14.21±0.22 6.33±0.55 1.51±0.13 29.39±1.01
9d 13.25±0.21 5.51±0.31 1.27±0.07 33.95±2.34
12d 12.89±0.09 4.55±0.28 1.19±0.14 38.88±3.05
From table 2 it can be seen that the growing state of pepper seedling can be substantially improved by spraying methyl jasmonate solution.
The above-described embodiments are merely illustrative of preferred embodiments of the present invention, not to the structure of the present invention Think and range is defined.Under the premise of not departing from design concept of the present invention, technology of the ordinary people in the field to the present invention The all variations and modifications that scheme is made, should all drop into protection scope of the present invention, the claimed technology contents of the present invention, It has all recorded in detail in the claims.

Claims (5)

1. the application of methyl jasmonate, which is characterized in that the waterflooding that methyl jasmonate is applied to alleviate shallow root system vegetables injures;
The application process of the methyl jasmonate is:
Shallow root system vegetables foliage-spray methyl jasmonate solution during waterflooding or after waterflooding;It is molten continuously to spray methyl jasmonate Liquid 3-7 days;
A concentration of 0.3-2.0mmol L of the methyl jasmonate solution-1
The shallow root system vegetables are Solanaceae capsicum plants.
2. the application of methyl jasmonate according to claim 1, which is characterized in that the methyl jasmonate solution it is a concentration of 0.6-1.0mmol L-1
3. the application of methyl jasmonate according to claim 1, which is characterized in that the methyl jasmonate solution is to use body Add the aqueous solution that water obtains after the ethanol solution dissolving of a concentration of 75-95% of product.
4. the application of methyl jasmonate according to claim 1, which is characterized in that it is described spray during, continuously spray to It is dripped under liquid to having on shallow root system vegetables blade face.
5. the application of methyl jasmonate according to claim 1, which is characterized in that during waterflooding and waterflooding after to plant diseases The blade of leaf and 50% or more area jaundice is extractd, and carries out loosing soil.
CN201610317177.3A 2016-05-13 2016-05-13 Application of the methyl jasmonate on plant is waterlogging-resistant Active CN105961390B (en)

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CN108184990A (en) * 2018-02-09 2018-06-22 江苏沿海地区农业科学研究所 A kind of method for extending refrigeration cucumber freshness date using methyl jasmonate
CN108353678A (en) * 2018-04-23 2018-08-03 天津农学院 A method of improving aloe oxidation resistance with methyl jasmonate
CN110972798A (en) * 2019-12-27 2020-04-10 河南科技大学 Method for improving drought resistance of oil peony by using jasmonic acid
CN110972797A (en) * 2019-12-27 2020-04-10 河南科技大学 Method for improving drought resistance of oil peony by adopting methyl jasmonate

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US5118711A (en) * 1990-09-27 1992-06-02 International Flavors & Fragrances Inc. Ketone, ketoesters and alcohol in repelling insects; use of aliphatic ester in attracting insects and process and apparatus for determination of insect repellency and attractancy
CN1209017C (en) * 2002-04-23 2005-07-06 四川龙蟒福生科技有限责任公司 Plant growth regulator composition with cold-resisting action
GB0920893D0 (en) * 2009-11-27 2010-01-13 Syngenta Participations Ag Plant growth regulation

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Effective date of registration: 20221123

Address after: 850800 Zhacao County, Shannan City, Tibet Autonomous Region

Patentee after: Tibet Lvzhiyuan Modern Agricultural Technology Co.,Ltd.

Address before: 410000 in Mapoling Academy of Agricultural Sciences, Furong district, Changsha City, Hunan Province

Patentee before: HUNAN VEGETABLE Research Institute