CN110972798A - Method for improving drought resistance of oil peony by using jasmonic acid - Google Patents

Method for improving drought resistance of oil peony by using jasmonic acid Download PDF

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CN110972798A
CN110972798A CN201911387479.8A CN201911387479A CN110972798A CN 110972798 A CN110972798 A CN 110972798A CN 201911387479 A CN201911387479 A CN 201911387479A CN 110972798 A CN110972798 A CN 110972798A
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peony
oil
jasmonic acid
content
drought
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郭丽丽
侯小改
王菲
郭大龙
魏祯祯
张红晓
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Henan University of Science and Technology
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Henan University of Science and Technology
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
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  • Forests & Forestry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a method for improving drought resistance of oil peony by using jasmonic acid, which adopts jasmonic acid to induce oil peony to generate drought resistance; the method comprises the following steps: 1) selecting annual oil peony seedlings with consistent growth vigor and no diseases and insect pests for cultivation in 10 months; 2) culturing in greenhouse at room temperature for 5 months next year, and performing normal water management; 3) and (3) spraying jasmonic acid on the whole bead of the oil peony until the upper and lower surfaces of the leaf drip water, wherein the spraying is carried out once every 3d for 2 times, and the oil peony with drought resistance is obtained. In the post-growth stage of the peony for oil, exogenous JA with different concentrations is adopted for induction, so that the content of permeation regulating substances and the content of photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoid) of the peony for oil under drought stress are obviously improved, the content of malondialdehyde is reduced, the degree of cell membrane lipid peroxidation is reduced, the photosynthesis of the peony for paeonia ostii is promoted, and the defense capacity of the peony for oil under drought stress is enhanced.

Description

Method for improving drought resistance of oil peony by using jasmonic acid
Technical Field
The invention relates to the technical field of peony planting, in particular to a method for improving drought resistance of oil peony by using jasmonic acid.
Background
Paeonia suffruticosa (Paeonia suffruticosa) species, such as Paeonia ostii (Paeonia suffruticosa) and purpura (Paeonia rockii) with high seed setting rate and high oil yield, are called as oil peonies and are unique emerging woody oil plants in China. The peony seed oil is rich in linolenic acid, linoleic acid and other unsaturated fatty acids (Kim et al, 2014; Mao et al, 2017) which are necessary for human bodies and cannot be synthesized by the human bodies, simultaneously contains medicinal effective components of peony, has the effects of reducing blood sugar, blood fat, cholesterol and the like (Su et al, 2016), and can meet the requirements of people on high-quality vegetable oil. 2011 peony seed oil has been approved by the national health committee as a new resource food [2011 No. 9 ].
The peony for oil is the cultivar with the largest popularization area at present, the oil yield of seeds of the peony is up to 22 percent, the content of unsaturated fatty acid is up to 84 percent, the peony is rich in various linolenic acids and linoleic acid, the content of alpha-linolenic acid exceeds 42.35 percent, and the peony has extremely high nutritional value. Oil peonies in their habitat often encounter drought stress caused by insufficient water resources. The method is suitable for ecological conditions of global climate drought change and water resource shortage, develops novel oil crop peony which does not compete with grain and cotton in land under the basic national condition that the cultivated land area is limited, realizes the improvement of drought-resistant cultivation technology, and has important significance for realizing agricultural sustainable development.
Various methods are disclosed in the prior art for improving the drought resistance of oil peonies, for example, CN108849476A discloses a breeding method for improving the drought resistance of oil peonies, which combines the traditional hybridization mode with a chemical reagent treatment method, reduces the generation of false positive drought-resistant seeds, reduces the total sample amount of the subsequent hybridization steps, reduces the workload, and improves the breeding efficiency; patent CN106866241A discloses a breeding method for improving drought resistance of oil peony, which combines aging treatment and drought resistance agent to effectively improve germination rate of seeds, improve drought resistance of seeds and improve acre yield of oil peony. Although the prior art achieves the aim of improving the drought resistance of the oil peony, the prior art has the defects of high cost, complex process and long time consumption.
Therefore, how to provide a method for improving the drought resistance of oil peonies is a problem which needs to be solved by technical personnel in the field, and the technical problems in the prior art are solved.
Disclosure of Invention
In view of the above, the invention provides a method for improving the drought resistance of oil peonies by using jasmonic acid, aiming at the problem that the oil peonies are easily damaged by drought stress in the growth process and the normal growth of the oil peonies is influenced, so that the yield is reduced.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for improving drought resistance of oil peony by using jasmonic acid comprises inducing oil peony to generate drought resistance by using jasmonic acid; the method comprises the following steps:
1) selecting annual oil peony seedlings with consistent growth vigor and no diseases and insect pests for cultivation in 10 months;
2) culturing in greenhouse at room temperature for 5 months next year, and performing normal water management;
3) and (3) spraying jasmonic acid on the whole bead of the oil peony until the upper and lower surfaces of the leaf drip water, wherein the spraying is carried out once every 3d for 2 times, and the oil peony with drought resistance is obtained.
Preferably, the concentration of the jasmonic acid spraying in the step 3) is 50-200 mug/mL.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
1. the invention provides a method for improving the photosynthetic and physiological properties of annual oil peony seedlings under drought stress, and effectively solves the problem of poor growth of oil peonies under drought stress.
2. The method is simple to operate, and the content of Soluble Sugar (SS) and free proline (Pro) which are osmotic adjusting substances in the leaves can be improved by spraying exogenous jasmonic acid on the oil peony; by reducing the content of Malondialdehyde (MDA), the degree of membrane lipid peroxidation is reduced; increasing chlorophyll content (chlorophyll a, chlorophyll b, carotenoid), net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr) and intercellular CO2Concentration (Ci) to promote plantingCarrying out photosynthesis on the plants; reduce the damage of drought stress to oil peonies and improve the resistance of the oil peonies to the drought stress.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 shows the effect of jasmonic acid of different concentrations on the soluble sugar content of peony 'Paeonia ostii' for three-year-old oil under drought stress;
FIG. 2 shows the effect of jasmonic acid of different concentrations on proline content of peony 'Paeonia ostii' for three-year-old oil under drought stress;
FIG. 3 is a graph showing the effect of jasmonic acid of different concentrations on MDA content of peony 'Paeonia ostii' for three-year-old oil under drought stress;
FIG. 4 is a graph showing the effect of jasmonic acid of different concentrations on the chlorophyll a content of peony 'Paeonia ostii' for three-year-old oil under drought stress;
FIG. 5 shows the effect of jasmonic acid of different concentrations on the chlorophyll b content of peony 'Paeonia ostii' for three-year-old oil under drought stress;
FIG. 6 is a graph showing the effect of jasmonic acid of different concentrations on carotenoid content in three-year-old peony for oil under drought stress;
FIG. 7 is a graph of the effect of different concentrations of jasmonic acid on net photosynthetic rate of three-year old oil peony 'Paeonia ostii' under drought stress;
FIG. 8 is a graph showing the effect of jasmonic acid of different concentrations on stomatal conductance of tree-year-old peony 'Paeonia ostii' for oil under drought stress;
FIG. 9 shows intercellular CO of peony for three-year-old oil under drought stress with different concentrations of jasmonic acid2The effect of concentration;
FIG. 10 is a graph of the effect of different concentrations of jasmonic acid on the transpiration rate of three-year old oil peony 'Paeonia ostii' under drought stress.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The oil peony is a peony variety with high seed yield, high oil content and high effective components. Paeonia genus, Paeonia genus and Paeonia genus plant, 9 species of wild Paeonia species all over the world, and all distributed in China. At present, the natural growth state of wild peony can still be seen in Henan, Shaanxi, Hubei, Gansu, Sichuan, Yunnan, Tibet and the like; according to incomplete statistics, about 2000 species of peony varieties are commonly cultivated all over the world at present, more than 100 species of the peony varieties have certain fructification capability, but the peony varieties mainly comprise paeonia ostii and paeonia rockii which are used for cultivating oil peonies. The embodiment of the invention mainly takes paeonia ostii as an example, and the paeonia rockii variety can achieve the same technical effect.
Example 1
A method for improving drought resistance of oil peony by using Jasmonic Acid (JA) comprises the following steps:
the method comprises the following steps: three-year-old oil-bearing peony 'Paeonia ostii' plants which grow uniformly and uniformly are selected from an international peony garden and grafted, and the plants are planted in plastic flowerpots with the diameter of 20cm in 2017 and 10 months, wherein 1 plant is planted in each pot.
Step two: culturing in a plastic greenhouse for 5 months next year, and performing normal water management.
Step three: the whole plant spraying method is adopted, 50 mu mol/L jasmonic acid is adopted, the upper surface and the lower surface of the leaves are preferably dripped, and each treatment is set to be 5 times.
Step four: after 50 mu mol/L jasmonic acid treatment for 3d, drought stress (contrast, mild drought, severe drought and rehydration treatment) is carried out, the phenotypic change is observed every day, and the change of the photosynthetic performance index is measured.
Step five: the three-year oil peony 'paeonia ostii' is taken out of leaves under the conditions of contrast, mild drought, severe drought and rehydration respectively, is stored at minus 80 ℃ after being quickly frozen by liquid nitrogen, is used for change analysis of physiological and biochemical indexes, and is used for screening the jasmonic acid induction method for promoting the drought resistance of the oil peony.
Example 2
A method for improving drought resistance of oil peony by using Jasmonic Acid (JA) comprises the following steps:
the method comprises the following steps: three-year-old oil-bearing peony 'Paeonia ostii' plants which grow uniformly and uniformly are selected from an international peony garden and grafted, and the plants are planted in plastic flowerpots with the diameter of 20cm in 2017 and 10 months, wherein 1 plant is planted in each pot.
Step two: culturing in a plastic greenhouse for 5 months next year, and performing normal water management.
Step three: the whole plant spraying method is adopted, 100 mu mol/L jasmonic acid is adopted, the upper surface and the lower surface of the leaves are preferably dripped, and each treatment is set to be 5 times.
Step four: after 100 mu mol/L jasmonic acid treatment for 3d, drought stress (contrast, mild drought, severe drought and rehydration treatment) is carried out, the phenotypic change is observed every day, and the change of the photosynthetic performance index is measured.
Step five: the three-year oil peony 'paeonia ostii' is taken out of leaves under the conditions of contrast, mild drought, severe drought and rehydration respectively, is stored at minus 80 ℃ after being quickly frozen by liquid nitrogen, is used for change analysis of physiological and biochemical indexes, and is used for screening the jasmonic acid induction method for promoting the drought resistance of the oil peony.
Example 3
A method for improving drought resistance of oil peony by using Jasmonic Acid (JA) comprises the following steps:
the method comprises the following steps: three-year-old oil-bearing peony 'Paeonia ostii' plants which grow uniformly and uniformly are selected from an international peony garden and grafted, and the plants are planted in plastic flowerpots with the diameter of 20cm in 2017 and 10 months, wherein 1 plant is planted in each pot.
Step two: culturing in a plastic greenhouse for 5 months next year, and performing normal water management.
Step three: the whole plant spraying method is adopted, 200 mu mol/L jasmonic acid is adopted, the upper surface and the lower surface of the leaves are preferably dripped, and each treatment is set to be 5 times.
Step four: after 200 mu mol/L jasmonic acid treatment for 3d, drought stress (contrast, mild drought, severe drought and rehydration treatment) is carried out, the phenotypic change is observed every day, and the change of the photosynthetic performance index is measured.
Step five: the three-year oil peony 'paeonia ostii' is taken out of leaves under the conditions of contrast, mild drought, severe drought and rehydration respectively, is stored at minus 80 ℃ after being quickly frozen by liquid nitrogen, is used for change analysis of physiological and biochemical indexes, and is used for screening the jasmonic acid induction method for promoting the drought resistance of the oil peony.
Example 4
A method for improving drought resistance of oil peony by using Jasmonic Acid (JA) comprises the following steps:
the method comprises the following steps: three-year-old oil-bearing peony 'Paeonia ostii' plants which grow uniformly and uniformly are selected from an international peony garden and grafted, and the plants are planted in plastic flowerpots with the diameter of 20cm in 2017 and 10 months, wherein 1 plant is planted in each pot.
Step two: culturing in a plastic greenhouse for 5 months next year, and performing normal water management.
Step three: the whole plant spraying method is adopted, 400 mu mol/L jasmonic acid is adopted, the upper surface and the lower surface of the leaves are preferably dripped, and each treatment is repeated for 5 times.
Step four: after the treatment of 400 mu mol/L jasmonic acid for 3d, drought stress (contrast, mild drought, severe drought and rehydration treatment) is carried out, the phenotypic change is observed every day, and the change of the photosynthetic performance index is measured.
Step five: the three-year oil peony 'paeonia ostii' is taken out of leaves under the conditions of contrast, mild drought, severe drought and rehydration respectively, is stored at minus 80 ℃ after being quickly frozen by liquid nitrogen, is used for change analysis of physiological and biochemical indexes, and is used for screening the jasmonic acid induction method for promoting the drought resistance of the oil peony.
Related experiments: treatment of jasmonic acid solutions with different concentrations under drought stress
The test sets an absolute control, a relative control and a treatment group, wherein the absolute control is a tree peony 'paeonia ostii' plant for three-year-old oil which is not sprayed with any concentration JA in a natural state, the relative control is a tree peony 'paeonia ostii' plant for three-year-old oil which is sprayed with different concentrations JA in a natural state but is not subjected to drought stress, and the treatment group is a tree peony 'paeonia ostii' plant for three-year-old oil which is sprayed with different concentrations JA and is subjected to different degrees of drought stress and rehydration treatment. Finally, the leaves of Paeonia ostii Paeonia suffruticosa under different treatments are collected, and are stored at minus 80 ℃ for later use after being quickly frozen by liquid nitrogen.
Under different treatment conditions, the change of photosynthetic property of peony 'Paeonia ostii' for three-year oil is measured by Li-6400 portable photosynthesizer (Li-COR, Lincoln, Nebraska, USA), and the measurement index mainly comprises net photosynthetic rate (P)n) And gas pore conductivity (G)s) Intercellular CO2Concentration (Intercellular CO)2concentration，Ci) Transpiration rate transfer rate, Tr) And the like. The photosynthetic pigment content is determined by ethanol extraction method, and the determination indexes mainly comprise chlorophyll a (chlorophenyll a), chlorophyll b (chlorophenyll b) and Carotenoid (Carotenoid).
Under different treatment conditions, the change of the content of Malondialdehyde (MDA), Soluble Sugar (SS) and Proline (Pro) is mainly determined by the change analysis of the physiological characteristics of the peony 'paeonia ostii' for the three-year-old oil. And (3) measuring the content change of MDA by adopting a thiobarbituric acid method, measuring the content change of soluble sugar by adopting an anthrone ethyl acetate method, and measuring the content change of proline by adopting an acidic indetrione method. All test criteria were set to 5 replicates. Excel and SPSS19.0 software is adopted for data arrangement, correlation analysis and principal component analysis, and origin8.0 software is adopted for drawing.
As shown in figure 1, the content of soluble sugar in leaves of Paeonia ostii under drought stress treatment is rapidly increased, and the damage of drought to membrane permeability is serious. After exogenous JA is sprayed, the soluble sugar content of the 'paeonia ostii' leaves is reduced under different drought stress degrees, the soluble sugar content of the leaves after mild drought, moderate drought and rehydration is respectively reduced by 27.2%, 1.3% and 2.2% compared with that of a Control (CK) under the condition that the JA concentration is 100 mu mol/L, and the soluble sugar content is obviously different from that of the control (P is less than 0.05) under different JA concentration treatments, which shows that the soluble sugar content of the 'paeonia ostii' leaves can be improved by the JA treatment with proper concentration.
As shown in fig. 2, the proline content of the leaf of the paeonia ostii is obviously increased (P <0.05) by spraying exogenous JA with different concentrations. Along with the increase of the stress degree, the proline content of the Paeonia ostii' leaves is in a gradually rising trend, the proline content of the leaves is rapidly increased under the moderate drought stress, and the cell osmotic potential can be improved and the stability of a membrane structure can be enhanced by JA treatment.
As shown in FIG. 3, the content of MDA in leaves of Paeonia ostii under drought stress is greatly increased, and the degree of lipid peroxidation of the cell membranes of the leaves of Paeonia ostii is increased due to drought. After exogenous JA is sprayed, the MDA content is reduced compared with a control group under different drought stress degrees, and the content tends to be increased firstly and then reduced along with the increase of the JA concentration, which shows that exogenous JA has obvious inhibition effect on the MDA content. The MDA content after drought stress and rehydration is lowest when the concentration is 50 mu mol/L, and is respectively reduced by 86.1%, 45.8% and 10.7% compared with a control group (CK), and the MDA content of the leaves of the Paeonia ostii is obviously different from the control group (P <0.05) under the treatment of JA with different concentrations. JA can inhibit the peroxidation of cell membrane lipid of peony to a certain extent and maintain the normal growth of Paeonia ostii' peony in adverse circumstances.
As shown in FIG. 4, under moderate and severe drought stress and rehydration conditions, the chlorophyll a content of the Paeonia ostii' leaves is increased by 7.9%, 46.3% and 4.2% respectively compared with that of absolute control. After exogenous JA treatment, under mild and severe drought and rehydration, the chlorophyll a content is in a trend of rising firstly and then falling, the maximum value is reached when the JA concentration is 100 mu mol/L, and the chlorophyll a content is respectively increased by 10.1%, -17.6%, 8.6% and 7.4% compared with the control. The content of chlorophyll a is slightly higher than that of a control in severe drought treatment with JA concentration of 100 mu mol/L. Under the condition of the concentration, the content of the chlorophyll a subjected to rehydration is higher than that of a control, and the condition that 100 mu mol/L JA treatment can delay the degradation of the chlorophyll a under drought stress is shown.
As shown in fig. 5, JA suppressed the increase in chlorophyll b content under drought stress, and as the concentration of JA increased, the chlorophyll content tended to increase first and then decrease. Under severe drought stress degree, when the JA concentration is 100 mu mol/L, the chlorophyll b content is highest and is increased by 43.3 percent compared with a control; under the rehydration condition, when the JA concentration is 200 mu mol/L, the chlorophyll b content is remarkably reduced by 122.8%.
As shown in FIG. 6, under exogenous JA treatment conditions, the carotenoid content increased first and then decreased with increasing JA concentration, and the carotenoid content in the leaves of Paeonia ostii reaches the maximum value when the JA concentration is 100. mu. mol/L, which is respectively higher than that of control samples 7.5%, -0.03%, 11.8% and 13.4%. The exogenous JA with proper concentration can increase the content of photosynthetic pigments and improve the light energy capture efficiency of the peony 'paeonia ostii' for oil.
As shown in fig. 7, the net photosynthetic rate of the leaves of paeonia ostii under drought stress decreased significantly. When the exogenous JA is sprayed under different drought stress treatment conditions, the net photosynthetic rate is increased compared with that of a control, and the net photosynthetic rate tends to increase firstly and then decrease along with the increase of the concentration of the JA, which shows that the exogenous JA has obvious promotion effect on the increase of the net photosynthetic rate. Under different drought stress and rehydration conditions, the net photosynthetic rate of the Paeonia ostii' leaves reaches the maximum value when the JA concentration is 100 mu mol/L, and is respectively increased by 172.0%, 89.9% and 90.7% compared with the control. Thus. Exogenous JA induction has certain promotion effect on the improvement of the net photosynthetic rate of the oil peony 'paeonia ostii' under drought stress.
As shown in fig. 8, 'paeonia ostii' has a significant decrease in stomatal conductance under drought stress (P < 0.05). The stomatal conductance of Paeonia ostii is increased and then decreased by JA treatment, and the stomatal conductance of Paeonia ostii is maximized under different drought stress degrees and rehydration by 100 mu mol/L exogenous JA induction, which is increased by 320.3%, 115% and 188.6% respectively compared with the control. When the JA concentration is more than 200 mu mol/L, the stomatal conductance of the Paeonia ostii leaf shows a descending trend under moderate drought, which shows that the increasing effect of high-concentration JA on the stomatal conductance of Paeonia ostii is not obvious.
As shown in FIG. 9, intercellular CO increases with the degree of stress2The concentration is rapidly reduced and is recovered after rehydration. Under different drought stress treatments after different concentrations of JA induction, 'Paeonia ostii' leaf intercellular CO2The concentration changes were small but all increased. When the JA concentration is 400 mu mol/L, the different drought stress treatments are respectively increased by 20.5 percent, 9.8 percent and 37.9 percent compared with the control,the concentration of JA increases and then decreases, which shows that the induction of JA with proper concentration can promote intercellular CO2The concentration is increased to improve the photosynthetic capacity of the leaves.
As shown in fig. 10, 'paeonia ostii' has a significant decrease in transpiration rate under drought stress. The JA treatment tends to increase and decrease the transpiration rate. After different degrees of drought stress and rehydration, 100 mu mol/L JA treatment enables the transpiration rate of the leaves of the paeonia ostii to reach the maximum value, and the transpiration rate is respectively increased by 277.3%, 111.7% and 165.5% compared with the control. The increase of the transpiration rate can promote the water transportation of the plant body and promote the water absorption capacity of the root system.
In conclusion, the harm of the peony 'paeonia ostii' for the three-year-old oil under the drought stress can be correspondingly relieved by adopting exogenous JA induction with proper concentration, the relieving effect is different due to different JA concentrations, and the best relieving effect of externally applying 100 mu mol/L JA on the drought stress of the peony 'paeonia ostii' for the three-year-old oil is achieved.
In the post-growth stage of the peony for oil, exogenous JA with different concentrations is adopted for induction, so that the content of permeation regulating substances and the content of photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoid) of the peony under drought stress are obviously improved, the content of malondialdehyde is reduced, the degree of cell membrane lipid peroxidation is reduced, the photosynthesis of the peony is promoted, and the defense capacity of the peony against drought stress is enhanced.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description. The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (2)

1. A method for improving the drought resistance of oil peony by using jasmonic acid is characterized in that the jasmonic acid is used for inducing the oil peony to generate drought resistance; the method comprises the following steps:
1) selecting annual oil peony seedlings with consistent growth vigor and no diseases and insect pests for cultivation in 10 months;
2) culturing in greenhouse at room temperature for 5 months next year, and performing normal water management;
3) and (3) spraying jasmonic acid on the whole bead of the oil peony until the upper and lower surfaces of the leaf drip water, wherein the spraying is carried out once every 3d for 2 times, and the oil peony with drought resistance is obtained.
2. The method for improving the drought resistance of oil peonies by using jasmonic acid according to claim 1, wherein the concentration of the jasmonic acid sprayed in the step 3) is 50-200 μ g/mL.
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CN111820238A (en) * 2020-06-09 2020-10-27 扬州大学 Drought-resistant agent for relieving drought stress of paeonia ostii and application thereof

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