CN113287613A - Plant growth regulator for enhancing drought resistance of cauliflowers and preparation method and application thereof - Google Patents
Plant growth regulator for enhancing drought resistance of cauliflowers and preparation method and application thereof Download PDFInfo
- Publication number
- CN113287613A CN113287613A CN202110482418.0A CN202110482418A CN113287613A CN 113287613 A CN113287613 A CN 113287613A CN 202110482418 A CN202110482418 A CN 202110482418A CN 113287613 A CN113287613 A CN 113287613A
- Authority
- CN
- China
- Prior art keywords
- plant growth
- growth regulator
- concentration
- methylcyclopropene
- gibberellin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a plant growth regulator for enhancing drought resistance of cauliflowers and a preparation method and application thereof, wherein the effective components of the plant growth regulator comprise jasmonic acid, 1-methylcyclopropene and gibberellin, the concentration of the jasmonic acid is 0.1-1mg/L, the concentration of the 1-methylcyclopropene is 1-5mg/L, and the concentration of the gibberellin is 1-10 mg/L. The plant growth regulator provided by the invention can improve the photosynthesis of cauliflower seedlings after drought stress treatment; the chlorophyll content is increased, the formation of strong seedlings can be promoted, and the damage caused by drought stress can be resisted to a certain extent; improving the content of soluble protein in the cauliflower seedling leaves subjected to drought stress; after drought stress treated by the plant growth regulator, the SOD activity of seedlings is obviously increased, the MDA content is obviously reduced, and the injury degree of cauliflower seedlings caused by drought is reduced.
Description
Technical Field
The invention belongs to the field of plant growth regulators, and particularly relates to a plant growth regulator for enhancing drought resistance of cauliflowers, and a preparation method and application thereof.
Background
The chemical control technology is an effective, simple and easy method for controlling the loss of crops caused by natural conditions. The study of adapting plants to adversity survival by inducing gene expression, particularly resistance gene expression, with signal substances such as hormone compounds has been a common approach for those skilled in the art.
Cauliflower, also known as cauliflower, cauliflower or broccoli, is an annual plant of brassica genus of Brassicaceae, also known as cauliflower, broccoli, cabbage, gelidium amansii, broccoli. Because the vitamin C content is very rich, the compound also has the anticancer effect and is deeply favored by people. Foreign researches also find that the cauliflower contains various indole derivatives, can reduce the estrogen level and can prevent the occurrence of breast cancer. The patients with gastrointestinal cancer and breast cancer due to spleen deficiency and stomach weakness should advocate to eat more cauliflowers.
However, since cauliflower belongs to semi-cold-resistant vegetables, hot and drought are avoided, and the cauliflower is not frost-resistant, the growing proper temperature range is narrow, the cold resistance is poor, the requirements on temperature and water control in planting are strict, and planting obstacles are caused.
Disclosure of Invention
In view of the above, the invention provides a plant growth regulator for enhancing drought resistance of cauliflowers, and a preparation method and application thereof, aiming at overcoming the defects in the prior art.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
a plant growth regulator comprises jasmonic acid, 1-methylcyclopropene, and gibberellin as effective components, wherein the concentration of jasmonic acid is 0.1-1mg/L, the concentration of 1-methylcyclopropene is 1-5mg/L, and the concentration of gibberellin is 1-10 mg/L.
Preferably, the concentration of the jasmonic acid is 1mg/L, the concentration of the 1-methylcyclopropene is 5mg/L, and the concentration of the gibberellin is 10 mg/L.
Preferably, the plant growth regulator further comprises an auxiliary ingredient, and the auxiliary ingredient comprises at least one of an organic solvent, an emulsifier, a wetting dispersant, a thickener, an antifreeze, an antifoaming agent, a binder, a disintegrant, water and a solid inert carrier.
The invention also provides a preparation method of the plant growth regulator, which comprises the following steps: the raw materials in the weight ratio are uniformly mixed and are prepared into missible oil, spray, dilutable solution, dilutable emulsion, wettable powder, soluble powder, suspending agent, granules or microcapsules with auxiliary agents allowed in pesticides.
The invention also provides application of the plant growth regulator in enhancing stress resistance of cruciferous plants.
Preferably, the stress resistance is drought resistance.
Preferably, the cruciferous plant is broccoli.
Compared with the prior art, the invention has the following advantages:
the plant growth regulator can improve the photosynthesis of the cauliflower seedlings after drought stress treatment; the chlorophyll content is increased, the formation of strong seedlings can be promoted, and the damage caused by drought stress can be resisted to a certain extent; improving the content of soluble protein in the cauliflower seedling leaves subjected to drought stress; the SOD activity of seedlings is obviously increased after drought stress treated by the plant growth regulator, the MDA content is obviously reduced, the injury degree of cauliflower seedlings caused by drought is reduced, the drought resistance of the cauliflower is improved from the above aspects, a foundation is provided for the compounding of three components, namely jasmonic acid, 1-methylcyclopropene and gibberellin, so that the drought resistance of the cauliflower is improved through induction, and the plant growth regulator can be comprehensively popularized and applied.
Detailed Description
Unless defined otherwise, technical terms used in the following examples have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.
The invention will be described in detail with reference to the following examples.
Example 1
Adding 25mg of jasmonic acid and 1000g of propylene glycol into a reaction kettle with the capacity of 50L, and stirring for 20 minutes; then adding 50mg of 1-methylcyclopropene, stirring for 30 minutes until the 1-methylcyclopropene is completely dissolved, and then adding 50mg of gibberellin; adding 3000g of Arabic gum, and adding the balance of water to 50L, and stirring uniformly to obtain the plant growth regulator aqueous solution with jasmonic acid concentration of 0.5mg/L, 1mg/L of 1-methylcyclopropene and 1mg/L of gibberellin.
Example 2
Adding 5mg of jasmonic acid and 1000g of propylene glycol into a reaction kettle with the capacity of 50L, and stirring for 20 minutes; then 100mg of 1-methylcyclopropene is added, the mixture is stirred for 30 minutes until the 1-methylcyclopropene is completely dissolved, and then 250mg of gibberellin is added; adding 3000g of Arabic gum, and adding the balance of water to 50L, and stirring uniformly to obtain the plant growth regulator aqueous solution with jasmonic acid concentration of 0.1mg/L, 1-methylcyclopropene concentration of 2mg/L and gibberellin concentration of 5 mg/L.
Example 3
Adding 25mg of jasmonic acid and 1000g of propylene glycol into a reaction kettle with the capacity of 50L, and stirring for 20 minutes; adding 250mg of 1-methylcyclopropene, stirring for 30 minutes until the 1-methylcyclopropene is completely dissolved, and then adding 400mg of gibberellin; adding 3000g of Arabic gum, and adding the balance of water to 50L, and stirring uniformly to obtain the plant growth regulator aqueous solution with jasmonic acid concentration of 0.5mg/L, 1-methylcyclopropene concentration of 5mg/L and gibberellin concentration of 8 mg/L.
Example 4
Adding 50mg of jasmonic acid and 1000g of propylene glycol into a reaction kettle with the capacity of 50L, and stirring for 20 minutes; adding 250mg of 1-methylcyclopropene, stirring for 30 minutes until the 1-methylcyclopropene is completely dissolved, and then adding 500mg of gibberellin; adding 3000g of Arabic gum, and adding the balance of water, and uniformly stirring to obtain the plant growth regulator aqueous solution with the jasmonic acid concentration of 1mg/L, the 1-methylcyclopropene concentration of 5mg/L and the gibberellin concentration of 10 mg/L.
Comparative example 1
Adding 50mg of jasmonic acid and 1000g of propylene glycol into a reaction kettle with the capacity of 50L, and stirring for 20 minutes; and adding 250mg of 1-methylcyclopropene, stirring for 30 minutes until the 1-methylcyclopropene is completely dissolved, adding 3000g of Arabic gum, and adding the balance of water to complement, and uniformly stirring to obtain the plant growth regulator aqueous solution with the jasmonic acid concentration of 1mg/L and the 1-methylcyclopropene concentration of 5 mg/L.
Comparative example 2
Adding 250mg of 1-methylcyclopropene and 1000g of propylene glycol into a reaction kettle with the capacity of 50L, and stirring for 20 minutes; then adding the mixture, stirring the mixture for 30 minutes until the 1-methylcyclopropene is completely dissolved, and then adding 500mg of gibberellin; adding 3000g of Arabic gum, and adding the balance of water, and uniformly stirring to obtain the plant growth regulator aqueous solution with the concentration of 1-methylcyclopropene of 5mg/L and the concentration of gibberellin of 10 mg/L.
Comparative example 3
Adding 50mg of jasmonic acid and 1000g of propylene glycol into a reaction kettle with the capacity of 50L, stirring for 20 minutes, and then adding 500mg of gibberellin; adding 3000g of Arabic gum, and adding the balance of water, and uniformly stirring to obtain the plant growth regulator aqueous solution with the jasmonic acid concentration of 1mg/L and the gibberellin concentration of 10 mg/L.
Comparative example 4
Adding 500mg of gibberellin and 1000g of propylene glycol into a 50L reaction kettle, adding 3000g of Arabic gum, and adding the balance of water to complement, and uniformly stirring to obtain the plant growth regulator aqueous solution with the gibberellin concentration of 10 mg/L.
Comparative example 5
Adding 50mg of jasmonic acid and 1000g of propylene glycol into a reaction kettle with the capacity of 50L, adding 3000g of Arabic gum, and adding the balance of water to complement, and uniformly stirring to obtain the plant growth regulator aqueous solution with the jasmonic acid concentration of 1 mg/L.
Comparative example 6
Adding 250mg of 1-methylcyclopropene and 1000g of propylene glycol into a 50L reaction kettle, adding 3000g of Arabic gum, and adding the balance of water to complement, and uniformly stirring to obtain the plant growth regulator aqueous solution with the concentration of the 1-methylcyclopropene of 5 mg/L.
Test examples
The test material is selected from a Cauliflower of Seisan variety purchased from vegetable research institute of Nongkovich, Tianjin;
jasmonic acid is purchased from Shanghai Michelle chemical technology Co., Ltd, and the product number is TRC-J210520;
gibberellins were purchased from Zhengzhou agricultural science and technology Limited for wet rain and were 95% pure.
1-methylcyclopropene was purchased from AgroFreesh, USA.
Test method
The cauliflower seeds are put into 75% ethanol for soaking for 10min, then washed by distilled water for a plurality of times, and sowed in black (12cm multiplied by 12cm) seedling pots. The potting soil is formed by mixing turfy soil, vegetable garden soil and vermiculite (volume ratio of 1: 1).
When the 4 th true leaf of the seedling is unfolded, drought stress treatment is carried out. The relative moisture content (RWC) of the leaves is maintained at 55-65%. The relative water content of the normally growing leaves is 85-90%. Normal water supply was used as control (CK 0). Simultaneously with the drought treatment, the aqueous solutions of examples 1-4 and comparative examples 1-6 were sprayed on the leaf surface for 2 days. The drought stress control is obtained by spraying the same amount of clear water. Each treatment was repeated 3 times. Sampling is respectively carried out on the 16 th day of continuous drought, and the sampling time is 8: 00-10: 00 in the morning. The 5 th or 6 th leaf which is completely unfolded is used as a physiological and biochemical analysis sample. Immediately after sampling, the sample is quickly frozen for 30min by using liquid nitrogen, and then the sample is transferred to a low-temperature refrigerator at the temperature of minus 40 ℃ for storage and standby test. The photosynthetic rate and chlorophyll content of each group, and the soluble protein content, superoxide dismutase (SOD) activity and Malondialdehyde (MDA) content of the leaves were measured, respectively, and the results are shown in the following table.
As can be seen from the above table, the plant growth regulator of the present invention can improve photosynthesis of cauliflower seedlings after drought stress treatment; the chlorophyll content is increased, the formation of strong seedlings can be promoted, and the damage caused by drought stress can be resisted to a certain extent; improving the content of soluble protein in the cauliflower seedling leaves subjected to drought stress; after drought stress treated by the plant growth regulator, the SOD activity of seedlings is obviously increased, the MDA content is obviously reduced, and the injury degree of cauliflower seedlings caused by drought is reduced.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the invention, so that any modifications, equivalents, improvements and the like, which are within the spirit and principle of the present invention, should be included in the scope of the present invention.
Claims (7)
1. A plant growth regulator characterized by: the active ingredients of the compound comprise jasmonic acid, 1-methylcyclopropene and gibberellin, wherein the concentration of the jasmonic acid is 0.1-1mg/L, the concentration of the 1-methylcyclopropene is 1-5mg/L, and the concentration of the gibberellin is 1-10 mg/L.
2. A plant growth regulator according to claim 1, characterized in that: the concentration of the jasmonic acid is 1mg/L, the concentration of the 1-methylcyclopropene is 5mg/L, and the concentration of the gibberellin is 10 mg/L.
3. A plant growth regulator according to claim 1, characterized in that: the plant growth regulator also comprises auxiliary components, wherein the auxiliary components comprise at least one of organic solvent, emulsifier, wetting dispersant, thickener, antifreeze, defoamer, binder, disintegrant, water and solid inert carrier.
4. A method for preparing a plant growth regulator according to claims 1 to 3, characterized in that: the method comprises the following steps: the raw materials in the weight ratio are uniformly mixed and are prepared into missible oil, spray, dilutable solution, dilutable emulsion, wettable powder, soluble powder, suspending agent, granules or microcapsules with auxiliary agents allowed in pesticides.
5. Use of the plant growth regulator of claims 1-3 to enhance stress tolerance in crucifers.
6. Use according to claim 5, characterized in that: the stress resistance is drought resistance.
7. Use according to claim 5, characterized in that: the method is characterized in that: the cruciferous plant is cauliflower.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110482418.0A CN113287613A (en) | 2021-04-30 | 2021-04-30 | Plant growth regulator for enhancing drought resistance of cauliflowers and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110482418.0A CN113287613A (en) | 2021-04-30 | 2021-04-30 | Plant growth regulator for enhancing drought resistance of cauliflowers and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113287613A true CN113287613A (en) | 2021-08-24 |
Family
ID=77321708
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110482418.0A Pending CN113287613A (en) | 2021-04-30 | 2021-04-30 | Plant growth regulator for enhancing drought resistance of cauliflowers and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113287613A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118125877A (en) * | 2024-03-06 | 2024-06-04 | 烟台凡西农业科技有限公司 | Alginate oligosaccharide plant stress-resistant auxin, preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1314084A (en) * | 2001-03-30 | 2001-09-26 | 中国科学院水利部水土保持研究所 | Crop drought-resistant agent and preparation method thereof |
| CN101715760A (en) * | 2009-12-01 | 2010-06-02 | 山东营养源食品科技有限公司 | 1-methylcyclopropene liquid preparation |
| CN105557711A (en) * | 2015-12-15 | 2016-05-11 | 中国农业科学院作物科学研究所 | Low-temperature-resistant and drought-resistant seed treatment agent and preparation method thereof |
| CN111903420A (en) * | 2019-12-27 | 2020-11-10 | 河南科技大学 | Method for improving drought resistance of oil peony by using jasmonic acid |
| CN112425604A (en) * | 2020-11-26 | 2021-03-02 | 西南大学 | Seed initiator and method for improving vigor and drought resistance of sweet sorghum seeds in germination period |
-
2021
- 2021-04-30 CN CN202110482418.0A patent/CN113287613A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1314084A (en) * | 2001-03-30 | 2001-09-26 | 中国科学院水利部水土保持研究所 | Crop drought-resistant agent and preparation method thereof |
| CN101715760A (en) * | 2009-12-01 | 2010-06-02 | 山东营养源食品科技有限公司 | 1-methylcyclopropene liquid preparation |
| CN105557711A (en) * | 2015-12-15 | 2016-05-11 | 中国农业科学院作物科学研究所 | Low-temperature-resistant and drought-resistant seed treatment agent and preparation method thereof |
| CN111903420A (en) * | 2019-12-27 | 2020-11-10 | 河南科技大学 | Method for improving drought resistance of oil peony by using jasmonic acid |
| CN112425604A (en) * | 2020-11-26 | 2021-03-02 | 西南大学 | Seed initiator and method for improving vigor and drought resistance of sweet sorghum seeds in germination period |
Non-Patent Citations (1)
| Title |
|---|
| 孟繁静: "《植物生理学》", 30 September 2000 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118125877A (en) * | 2024-03-06 | 2024-06-04 | 烟台凡西农业科技有限公司 | Alginate oligosaccharide plant stress-resistant auxin, preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110881468B (en) | Crop growth regulator composition and application thereof | |
| US4212664A (en) | Nicotinamide-ammonium hydroxide plant growth regulator compositions | |
| CN112514909A (en) | Yield increasing composition, yield increasing agent and application thereof | |
| CN110959617A (en) | Plant growth stress protective agent and use method thereof | |
| CN103202315A (en) | Low-temperature-resistant modifier of wheat and preparation method of modifier | |
| WO2017166566A1 (en) | Plant growth regulating composition | |
| CN113287613A (en) | Plant growth regulator for enhancing drought resistance of cauliflowers and preparation method and application thereof | |
| WO2020147277A1 (en) | Plant growth regulator composition | |
| CN111631224B (en) | Rice seed dressing agent and preparation method and application thereof | |
| CN110402962B (en) | Plant growth regulating composition and application thereof | |
| CN111011381B (en) | Seed treatment agent for improving lodging resistance and yield of quinoa | |
| KR100772849B1 (en) | Tiller promoter for plant and method for promoting tiller of plant | |
| CN110447647B (en) | Plant growth regulator and application thereof | |
| CN112293428A (en) | Synergistic composition containing organic silicon plant growth regulator | |
| CN113439747A (en) | Plant growth regulator composition containing brassinolide, chlorin iron and coronatine and application | |
| CN110637826A (en) | Plant growth regulator and preparation method and application thereof | |
| Mes | Gibberellic acid and the chilling requirements of peach seeds | |
| CN110402926B (en) | Plant growth regulator for promoting root growth and increasing yield and application thereof | |
| CN114304153B (en) | Cold-resistant, strong and lodging-resistant plant growth regulator and application thereof to crops | |
| US20220061314A1 (en) | Plant growth regulator in a semisolid or viscous medium | |
| CA1282976C (en) | Increasing the yield of cereals by means of brassinolide derivatives | |
| CN113016529A (en) | Application of Bibao in crop planting and crop planting method thereof | |
| CN115299441B (en) | Preparation for controlling excessive growth and promoting flowers as well as preparation method and application thereof | |
| Zhang et al. | Effects of Fulvic Acid on Agronomic Traits and Yield of Autumn Potato | |
| CN117378612A (en) | Rape seed dressing agent and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210824 |