CN110447647B - Plant growth regulator and application thereof - Google Patents
Plant growth regulator and application thereof Download PDFInfo
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- CN110447647B CN110447647B CN201910752014.1A CN201910752014A CN110447647B CN 110447647 B CN110447647 B CN 110447647B CN 201910752014 A CN201910752014 A CN 201910752014A CN 110447647 B CN110447647 B CN 110447647B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the field of agricultural plant protection, in particular to a plant growth regulator and application thereof. The active components of the plant growth regulator comprise an active component A and an active component B, wherein the active component A is 2, 4-difluorobenzoylaminoisobutyric acid, and the active component B is chitosan oligosaccharide; wherein the weight ratio of the active component A to the active component B is as follows: 1-40: 20-2000. Compared with a single agent, the plant growth regulator composition has more obvious effect, can enhance the stress resistance of crops, promote the growth of the crops, increase the yield of the crops and improve the fruit quality of the crops.
Description
Technical Field
The invention relates to the field of agricultural plant protection, in particular to a plant growth regulator and application thereof.
Background
The plant growth regulator is a hormone substance which is widely applied to agricultural production and used for regulating the growth and development of plants, can effectively regulate and control the growth and development of the plants, enhances the stress resistance, improves the yield and improves the quality, and has the characteristics of small dosage, quick response, less residue and strong pertinence. They are classified into plant growth promoters, plant growth inhibitors and plant growth retardants according to their effects on growth, and they are selected differently depending on the species of plants and the purpose of application. Common growth promoters include indole propionic acid, naphthalene acetic acid, 6-benzyl adenine; common growth inhibitors include triiodobenzoic acid, and cynarin; common growth retardants are uniconazole, paclobutrazol and chlormequat chloride. Researches show that two or more than two plant growth regulators are mixed or used in sequence, which often has better effect than single application and better plays the regulating and controlling role; however, the mixed use of two or more plant growth regulators can inhibit the growth of crops, and further, the yield of the crops is lower or the yield is improved by a smaller range than the use effect of a single agent, so that the reasonable use of the plant growth regulator is very important.
In recent years, plant growth regulators are receiving more and more attention, so that the agricultural cost can be reduced, the growth and the yield of crops can be improved, the fruit quality and the fertilizer utilization efficiency can be improved, the soil environment can be protected, and the occurrence of diseases can be reduced.
The invention content is as follows:
the invention aims to provide a plant growth regulator and application thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
a plant growth regulator comprises active components A and B, wherein the active component A is 2, 4-difluorobenzoylaminoisobutyric acid, and the active component B is chitosan oligosaccharide; wherein the weight ratio of the active component A to the active component B is as follows: 1-40: 20-2000.
The structural formula of the active component A2, 4-difluorobenzoyl aminoisobutyric acid is shown in the specificationThe molecular mass was 243.21.
The 2, 4-difluorobenzoylaminoisobutyric acid can be prepared according to the synthesis method disclosed in the patent document with the application number of 2018113654505, and the further synthesis steps are as follows:
a250 mL flask was charged with 7.0 g of 2, 4-difluorobenzoic acid, 10.0 g of EDCI (1-ethyl- (3-dimethylaminopropyl) carbonyldiimine hydrochloride), 7.5 g of methyl aminoisobutyrate hydrochloride, 150mL of dichloromethane, and 5mL of triethylamine. The reaction was stirred at room temperature for 12 hours. After the reaction, the reaction solution was washed with 50mL × 3 of water, the organic phase was dried over anhydrous sodium sulfate for 12 hours, desolventized under reduced pressure, and 150mL of ethanol and sodium hydroxide were added7.0 g, the reaction was stirred at room temperature for 2 hours. After the completion of the reaction monitored by TLC, the reaction was removed under reduced pressure, the residue was extracted with 100mL of water and 50 mL. times.3 with ethyl acetate, the organic phase was dried over anhydrous sodium sulfate for 12 hours, removed under reduced pressure, and purified by column chromatography (eluent was ethyl acetate) to give 2, 4-difluorobenzoylaminoisobutyric acid as a white solid (3.1 g).1H NMR(600MHz,DMSO)δ12.63(s,1H),8.55(s,1H),7.85(d,J=8.4Hz,1H),7.24(d,J=1.3Hz,1H),7.12(dd,J=8.4,1.6Hz,1H),1.52(s,6H)。
Preferably, the weight ratio of the active ingredient A to the active ingredient B is as follows: 1-20: 50-1000.
Further preferably, the weight ratio of the active ingredient A to the active ingredient B is as follows: 1-10: 100-1000.
The active ingredient, and an agriculturally acceptable adjuvant or carrier; wherein the concentration range of the active ingredient A is 0.005-0.2mg/L, and the concentration range of the active ingredient B is 0.1-10 mg/L.
The concentration range of the active component A is 0.01-0.2mg/L, and the concentration range of the active component B is 0.5-10 mg/L.
The concentration range of the active component A is 0.01-0.1mg/L, and the concentration range of the active component B is 1-10 mg/L.
The dosage form of the regulator is water solution, wettable powder, water dispersible granules, oil suspending agent, suspending emulsion, missible oil, microemulsion, aqueous emulsion, soluble granules or soluble powder.
Preferably, the dosage form of the regulator is water agent, wettable powder, soluble powder and suspending emulsion.
Use of a plant growth regulator for regulating the growth of a crop.
The crops are applied to wheat, rice, peanuts, potatoes or hot peppers.
The invention has the advantages that:
the active ingredients of the plant growth regulator, namely chitosan oligosaccharide and 2, 4-difluorobenzoyl aminoisobutyric acid, can meet the growth requirements of plants, promote the growth and development of the plants, improve the yield of the plants, improve the stress resistance of the crops, improve the quality of the crops and the like, and can not be left in the plant body, thereby improving the economic and social benefits; the method specifically comprises the following steps:
1. the active ingredients of the regulator are 2, 4-difluorobenzoyl aminoisobutyric acid and chitosan oligosaccharide, and the weight ratio of the active ingredients is 1-40: 20-2000 has obvious synergistic effect in regulating or promoting plant growth, can improve crop resistance and crop yield, improve crop quality and raise economic and social benefits.
2. Meanwhile, the use frequency and the use amount of the plant growth regulator of the invention are slightly changed with crops, growth environment and weather climate, and the purpose of increasing yield can be achieved by using proper dosage.
3. The plant growth regulator can enter the plant body through the stomata of the plant leaves, and can be decomposed through the physiological action in the plant body without being left in the plant body.
4. The plant growth regulator can promote the chlorophyll content of plants to rise, improve the photosynthetic efficiency, facilitate the accumulation of dry matters of the plants and further promote the yield to rise.
The specific implementation mode is as follows:
the invention is further described with reference to the following specific examples, but the invention is not limited to the following embodiments, and those skilled in the art will recognize that the invention encompasses all alternatives, modifications, and equivalents as may be included within the scope of the claims.
Formulation examples
According to the conventional preparation method of each preparation, each preparation takes the composition as an active ingredient, taking a water aqua as an example, the active ingredient accounts for 0.000101-0.00101% by weight, and auxiliary materials are prepared according to the weight percentage, wherein the active ingredients A and B are proportioned according to different concentrations, and the percentage of the active ingredient in the preparation is shown in brackets (table 1).
Table 1: 2, 4-difluorobenzoyl amino isobutyric acid and chitosan oligosaccharide compound
The addition amount of the auxiliary materials in each composition is 4 percent of sodium dodecyl benzene sulfonate, 1 percent of citric acid, 3 percent of polysorbate, 4 percent of butyl phosphate, 2 percent of ethylene glycol and pure water to make up to 100 percent.
Application example
1) Indoor pot experiment verification
Test subjects: gala small pepper (Harbin gold dragon agriculture company)
The test method comprises the following steps: the test was carried out in glass greenhouse of Shenyang chemical research institute. Mixing turfy soil (purchased from Shenyang south lake flower market) and sand uniformly according to a mass ratio of 1:1, loading the mixture into a seedling tray of 54cm x 27cm x 6cm, uniformly sowing seeds into the seedling tray, covering a soil layer with the thickness of about 0.5cm, watering from the bottom, and carrying out seed germination and seedling culture under the condition of abundant sunlight at 24-28 ℃. After pepper plants grow to 2 true leaves, selecting pepper seedlings with consistent growth vigor, transplanting the pepper seedlings into a flowerpot, keeping the growth conditions of each pot of seedlings consistent, applying base fertilizer after seedling delay, wherein the base fertilizer is conventional compound fertilizer 15-15-15, and the dosage is 600kg/hm2And (4) application. The first chemical spray was carried out 15 days after transplantation and the second chemical spray was carried out 30 days later, the pepper yield was investigated in the fruiting period, 5 replicates for each treatment, and the test results are shown in table 2.
The medicament is an experimental group reagent, a control group reagent and a blank; wherein, the experimental group is the aqueous agent which is prepared by compounding chitosan oligosaccharide and 2, 4-difluorobenzoyl amino isobutyric acid according to different methods and is described in the above embodiments; the control group is chitosan oligosaccharide or 2, 4-difluorobenzoylaminoisobutyric acid with different concentrations; the blank is clear water.
Table 2: pepper pot experiment determination data
As can be seen from Table 2, the results of the pot experiment show that the foliar spraying of chitosan oligosaccharide and 2, 4-difluorobenzoylaminoisobutyric acid can promote the increase of the pepper yield to different degrees, and the effect of the compound water aqua in the research is more obvious. As can be seen from the data in the table, when the ratio of 2, 4-difluorobenzoylaminoisobutyric acid to chitosan oligosaccharide is 1: the effect of 100 is most obvious, when the concentration of the chitosan oligosaccharide is 1mg/L and the concentration of the 2, 4-difluorobenzoylaminoisobutyric acid is 0.01mg/L, the yield of the pepper is most obvious, the yield increasing effect is higher than that of each single agent and other compound treatments, and the synergistic effect is obvious.
2) Pepper field test verification
Test subjects: chili pepper
The test method comprises the following steps: the test was carried out in 2018 in the western district of Xiyang city. Uniformly sowing the pepper seeds into a seedling raising tray, covering a layer of fine soil with the thickness of 0.5-1cm, and then pouring enough water. And when the pepper plants are 12cm long, selecting pepper seedlings with consistent growth vigor, and transplanting the pepper seedlings into the ground.
And (3) experimental design: the test cells were 5m long and 3m wide, and were arranged in random blocks of cells, with 3 replicates per test treatment. According to the test result of the pot culture, the experimental group is that the water aqua prepared by compounding the active ingredient A (2, 4-difluorobenzoyl amino isobutyric acid) and the active ingredient B (chitosan oligosaccharide) according to different concentrations in the water aqua prepared by the embodiment is prepared; meanwhile, the control group is active component A or active component B with different concentrations; the blank is clear water; after the slow seedling of the pepper, spraying different treatment agents for the first time, spraying for the second time after 30 days, and measuring the yield of the pepper in the harvest period, wherein the test results are shown in table 3.
Table 3: pepper field test determination data
As can be seen from Table 3, the yield measurement result of the hot pepper field is basically the same as the trend of the pot experiment, and the compounding effect is more obvious. Under 6 compound conditions, the yield of the pepper is remarkably increased, the yield increase amplitude is larger than the effect of spraying a single agent, wherein the ratio of 2, 4-difluorobenzoyl aminoisobutyric acid to chitosan oligosaccharide is 1: the yield increasing effect of 100 is greater than that of other proportioning conditions, and the ratio of the weight percent of the additive to the total weight percent of the fertilizer is 1: when the chitosan oligosaccharide concentration is 1mg/L and the 2, 4-difluorobenzoylaminoisobutyric acid concentration is 0.01mg/L under the condition of the mixture ratio of 100, the yield increase effect of the hot pepper field is most obvious, and the synergistic effect is obvious.
3) Peanut field test verification
Test subjects: peanut
The test method comprises the following steps: the test was carried out in 2018 in Daiwu county, Fuxin City, Liaoning province. The test material is hakko 3.
And (3) experimental design: the test cell length is 4m, the width is 1.65m, the row spacing is 55cm, the plant spacing is 10cm, the random block arrangement of the cell is provided, and 3 times of repetition are set for each test treatment. According to the test result of the pot culture, the experimental group is that the water aqua prepared by compounding the active ingredient A (2, 4-difluorobenzoyl amino isobutyric acid) and the active ingredient B (chitosan oligosaccharide) according to different concentrations in the water aqua prepared by the embodiment is prepared; meanwhile, the control group is active component A or active component B with different concentrations; the blank is clear water; the first spraying is carried out 15 days after sowing, the second spraying is carried out 30 days later, the SPAD value of peanut leaves and the dry fresh weight of plants are measured in the flowering period, the peanut yield is measured in the harvesting period, and the test results are shown in table 4.
Table 4: peanut field test determination data
As can be seen from Table 4, the field test results of peanuts show that the foliar spray of chitosan oligosaccharide and 2, 4-difluorobenzoylaminoisobutyric acid can promote the improvement of peanut yield, wherein the effect of the compound composition in the research is more obvious. Wherein the ratio of the 2, 4-difluorobenzoylaminoisobutyric acid to the chitosan oligosaccharide is 1: 100-1: the yield increasing effect of 200 is greater than that of other proportioning conditions, wherein the ratio of 1: when the concentration of the chitosan oligosaccharide is 1mg/L and the concentration of the 2, 4-difluorobenzoylaminoisobutyric acid is 0.01mg/L under the condition of a mixture ratio of 100, the accumulation of the chlorophyll content and the dry matter content of the peanut and the yield increasing effect are most obvious.
Therefore, the proportion of the 2, 4-difluorobenzoyl amino isobutyric acid and the chitosan oligosaccharide which are plant regulators is 1: 100-1: the yield increasing effect of the crops is more obvious when the yield is 200 hours, and the yield is 1: when the chitosan oligosaccharide is sprayed under the condition of 100 mixture ratio, the concentration of the chitosan oligosaccharide is 1mg/L, and the concentration of the 2, 4-difluorobenzoylaminoisobutyric acid is 0.01mg/L, the yield increasing effect of the pepper and the peanut is most obvious, wherein the yield of the pepper is increased by 17.53% and the yield of the peanut is increased by 15.62% in a field test, which shows that the plant growth regulator can promote the chlorophyll content of the plant to be increased, improve the photosynthetic efficiency, promote the accumulation and the transportation of dry matters of the plant, and further lead to the improvement of the yield of the peanut and the pepper.
Meanwhile, the obtained water aqua can also act on other crops, has obvious synergistic interaction effect in the aspect of regulating or promoting plant growth, and can improve the stress resistance and the crop yield of the crops, improve the crop quality and improve the economic and social benefits.
The above-described embodiments of the present invention should not be construed as limiting the scope of the present invention. Any other corresponding changes and modifications made according to the technical idea of the present invention should be included in the protection scope of the claims of the present invention.
Claims (9)
1. A plant growth regulator characterized by: the active components of the plant growth regulator comprise an active component A and an active component B, wherein the active component A is 2, 4-difluorobenzoylaminoisobutyric acid, and the active component B is chitosan oligosaccharide; wherein the weight ratio of the active component A to the active component B is as follows: 1-40: 50-2000;
2. A plant growth regulator according to claim 1, characterized in that: the weight ratio of the active component A to the active component B is as follows: 1-20: 50-1000.
3. A plant growth regulator according to claim 1, characterized in that: the weight ratio of the active component A to the active component B is as follows: 1-10: 100-1000.
4. A plant growth regulator according to any one of claims 1 to 3, characterized in that: the plant growth regulator consists of an active component of any one of claims 1 to 3 and an agriculturally acceptable adjuvant or carrier; wherein the concentration range of the active ingredient A is 0.005-0.2mg/L, and the concentration range of the active ingredient B is 0.1-10 mg/L.
5. A plant growth regulator according to claim 4, characterized in that: the concentration range of the active component A is 0.01-0.2mg/L, and the concentration range of the active component B is 0.5-10 mg/L.
6. A plant growth regulator according to claim 5, characterized in that: the concentration range of the active component A is 0.01-0.1mg/L, and the concentration range of the active component B is 1-10 mg/L.
7. A plant growth regulator according to claim 4, characterized in that: the dosage form of the regulator is water solution, wettable powder, water dispersible granules, oil suspending agent, suspending emulsion, missible oil, microemulsion, aqueous emulsion, soluble granules or soluble powder.
8. Use of a plant growth regulator according to any one of claims 1 to 4, characterized in that: the plant growth regulator is applied to regulating and controlling the growth of crops.
9. Use of a plant growth regulator according to claim 8, characterized in that: the crops are applied to wheat, rice, peanuts, potatoes or hot peppers.
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CN112006037A (en) * | 2020-09-04 | 2020-12-01 | 安徽省四达农药化工有限公司 | Plant growth regulator containing chitosan oligosaccharide and application thereof |
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