CN105961390A - Application of methyl jasmonate in waterlogging resistance of plants - Google Patents

Application of methyl jasmonate in waterlogging resistance of plants Download PDF

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Publication number
CN105961390A
CN105961390A CN201610317177.3A CN201610317177A CN105961390A CN 105961390 A CN105961390 A CN 105961390A CN 201610317177 A CN201610317177 A CN 201610317177A CN 105961390 A CN105961390 A CN 105961390A
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oxocyclopentylacetate
pent
enyl
methyl cis
waterflooding
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CN105961390B (en
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邹学校
欧立军
刘周斌
危革
张竹青
陈文超
戴雄泽
马艳青
杨博智
杨莎
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Tibet Lvzhiyuan Modern Agricultural Technology Co ltd
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INST OF VEGETABLES HUNAN PROV
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Life Sciences & Earth Sciences (AREA)
  • Ecology (AREA)
  • Engineering & Computer Science (AREA)
  • Forests & Forestry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention provides application of methyl jasmonate. The methyl jasmonate is applied to easing the waterflooding damage to shallow root vegetables so as to improve the waterlogging resistance of the shallow root vegetables. The application of the methyl jasmonate reduces the damage to the shallow root vegetables, thus improving the survival rate, guaranteeing the yield of the shallow root vegetables under the waterflooding condition, and increasing the income of farmers.

Description

(-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate application on plant is waterlogging-resistant
Technical field
The present invention relates to the application on shallow root system vegetable is waterlogging-resistant of a kind of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate, particularly relate to resist at Solanaceae capsicum plants Application in flood.
Background technology
Fructus Capsici (Capsicum annum L.) Solanaceae capsicum plants, one of vegetable species that China's cultivated area is maximum, every year Cultivated area reaches 1,300,000 more than hm2, belongs to shallow root system plant, and root system is thin and delicate, absorbs root less, when soil moisture is too much, The growth of Fructus Capsici can be affected.The harm of In Middle And Lower Reaches of Changjiang River flood stain frequently occurs, and has had a strong impact on spring and Xia Qiu outdoor cropping Fructus Capsici Yield and quality, and China plantation Fructus Capsici 60%-70% be outdoor cropping, damage or crop failure caused by waterlogging become domestic open country Fructus Capsici produce master Want one of stress factors.Fructus Capsici has stronger drought-resistance ability, but resistance to waterlogging ability is more weak, and Fructus Capsici, by after flood, easily occurs macerating root dead Seedling, yellowing leaf come off and the phenomenon such as fruit drop.Therefore, external measure is taked to improve the resistance to waterlogging ability of Fructus Capsici to promoting Fructus Capsici Spread and long season cultivation significant.Existing research shows external source Ca2+Can be by regulation pepper seedling root system The flooding stress injury to plant is alleviated in internal respiration metabolism, but it is only capable of a few days ago spraying one day after with waterflooding just effectively in waterflooding, And the less effective after spraying.
Although additionally, known (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate can improve the vigor of seed under cryopreservation, improving reply low temperature and the adverse circumstance such as saline and alkaline Ability, but the application similar with the present invention not yet occurred.
Summary of the invention
It is an object of the invention to provide the new application of a kind of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate, be applied to (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate alleviate shallow root system vegetables Dish waterflooding injures, and improves the waterlogging-resistant ability of shallow root system vegetable.
Technical scheme
The invention provides the application of a kind of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate, be applied to (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate alleviate shallow root system vegetable waterflooding injury.
The most described vegetable is Solanaceae capsicum plants.
Particularly preferred described vegetable is Fructus Capsici.
When described vegetable is Fructus Capsici, it is possible to obtain unexpected alleviation waterflooding injury and improve the good comprehensive of waterlogging-resistant ability Effect.
The application process of described (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate, the shallow root system vegetable foliage-spray (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate during waterflooding or after waterflooding is molten Liquid.
No matter the present invention is to spray (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate during waterflooding or after waterflooding, can obtain good alleviation waterflooding injury Effect.
Such as, the (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate spraying the present invention for after waterflooding 3-8 days also can obtain good effect.
The present invention comprises following preferred technical scheme:
Preferably in scheme, the concentration of described (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution is 0.3-2.0mmol L-1
The concentration of further preferred described (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution is 0.6-1.0mmol L-1
Preferably in scheme, spray (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution continuously 1-12 days.
Further preferably spray (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution continuously 3-7 days.
Described (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution is preferably with the aqueous solution obtained that adds water after ethanol solution dissolving.
Described spray during, spray the most continuously to shallow root system vegetable blade face and have lower of liquid.
The volumetric concentration of described ethanol solution is preferably 75-95%.
The blade preferably during waterflooding and after waterflooding turned to be yellow plant diseases leaf and more than 50% area is extractd, and carries out soil Loose.
Described (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution is preferably preserved by brown reagent bottle before spraying.
Although the present invention has the most only done the waterlogging-resistant experiment of Fructus Capsici, the starting point of research is for improving the waterlogging-resistant gentle of Fructus Capsici Solution waterflooding injures, it is contemplated that present invention may apply to the vegetables that general vegetable, particularly shallow root system are easily injured by waterflooding Dish.
Beneficial effects of the present invention
Although prior art having some other application of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate, but (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate can apply to alleviate plant Waterflooding injury is that inventor did not expect, the most never has relevant trial in prior art.
(-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate is sprayed the plant leaf surface during waterflooding or after waterflooding by inventor by accident, achieves unexpected good Good effect.
Present invention reduces the waterflooding injury to shallow root system vegetable, improve the survival rate of shallow root system vegetable, ensured flooding condition The yield of lower Fructus Capsici, adds peasant income.
In the concrete waterlogging-resistant experimentation of Fructus Capsici, inventor finds, especially by the concentration of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution and Spray the further optimization of natural law, it is possible to obtain more preferable plant height, root length, whole strain fresh weight, relative conductivity comprehensively improve effect, It is greatly improved the survival rate of Fructus Capsici.
By substantial amounts of experiment and test, inventor is found surprisingly that in the present invention and improves Fructus Capsici by spraying (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate Catalase, the activities of antioxidant enzymes such as superoxide dismutase, add osmotic adjustment proline content, reduce by the third two The accumulation of aldehyde and relative conductivity, improve the activity of anaerobic respiration relevant enzyme ethanol dehydrogenase simultaneously, and make root system keep higher Malic dehydrogenase, beneficially Fructus Capsici carry out aerobic respiration metabolism.
Accompanying drawing explanation
Fig. 1 is for spraying variable concentrations (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate to waterflooding Fructus Capsici MDA (malonaldehyde) content, Pro (aminoacid) content With CAT (catalase) affect figure.
Fig. 2 for spray variable concentrations (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate on waterflooding Fructus Capsici SOD (superoxide dismutase) activity affect figure.
Fig. 3 for spray variable concentrations (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate on waterflooding Fructus Capsici ADH (ethanol dehydrogenase) activity affect figure.
Fig. 4 for spray variable concentrations (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate on waterflooding Fructus Capsici MDH (malic dehydrogenase) activity affect figure.
Fig. 5 for spray different number of days (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate to waterflooding Fructus Capsici MDA (malonaldehyde) content, Pro (aminoacid) content and CAT (catalase) affects figure.
Fig. 6 for spray different number of days (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate on waterflooding Fructus Capsici SOD (superoxide dismutase) activity affect figure.
Fig. 7 for spray different number of days (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate on waterflooding Fructus Capsici ADH (ethanol dehydrogenase) activity affect figure.
Fig. 8 for spray different number of days (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate on waterflooding Fructus Capsici MDH (malic dehydrogenase) activity affect figure.
Detailed description of the invention
Embodiment 1
The application process of a kind of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate, comprises the following steps:
The first step, (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate is prepared
The (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate of 0.06729g, 0.13458g, 0.2243g, 0.33645g and 0.4486g is weighed by electronic balance, Pouring in the beaker of 1000mL, the ethanol of the 95% of addition 5mL dissolves (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate, then uses 200mL distilled water diluting The volumetric flask being placed on 1000mL is settled to 1000mL so that it is final concentration is respectively 0.3mmol L-1、0.6mmol L-1、 1.0mmol L-1、1.5mmol L-1With 2.0mmol L-1, preserve finally by brown reagent bottle.
Second step, foliage-spray during waterflooding
Waterflooding same day, the (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate of each concentration is placed in watering can so that it is nebulize ejection, sprays continuously to Fructus Capsici blade face, Until blade has lower of liquid, spray 5 days continuously.
3rd step, manages during waterflooding and after waterflooding
The blade during waterflooding and after waterflooding turned to be yellow pepper diseases leaf and more than 50% area is extractd, and carries out soil in period Earth loosens.
Table 1 sprays variable concentrations (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate to the impact of pepper seedling growth under flooding stress
As it can be seen from table 1 the growing state of pepper seedling can be substantially improved by spraying (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate.
Embodiment 2
The application process of a kind of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate, comprises the following steps:
The first step, (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate is prepared
Weigh 0.2243g (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate by electronic balance, pour in the beaker of 1000mL, the second of the 95% of addition 5mL Alcohol dissolves (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate, is then placed on the volumetric flask of 1000mL with 200mL distilled water diluting and is settled to 1000mL, makes Its final concentration is respectively 1.0mmol L-1, preserve finally by brown reagent bottle.
Second step, foliage-spray during waterflooding
Waterflooding same day, the (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate of each concentration is placed in watering can so that it is nebulize ejection, sprays continuously to Fructus Capsici blade face, Until blade has lower of liquid, spray 1 day, 3 days, 5 days, 7 days, 9 days and 12 days the most continuously.
3rd step, manages during waterflooding and after waterflooding
The blade during waterflooding and after waterflooding turned to be yellow pepper diseases leaf and more than 50% area is extractd, and carries out soil in period Earth loosens.
Table 2 difference sprays natural law (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate to the impact of pepper seedling growth under flooding stress
Process Plant height [cm] Root length [cm] Whole strain fresh weight [g] Relative conductivity [%]
Normal control 17.40±0.49 10.03±0.33 2.65±0.14 16.13±2.11
Simple submerging treatment 12.67±0.28 4.47±0.15 1.12±0.03 37.20±2.89
1d 12.29±0.15 5.01±0.15 1.32±0.12 36.97±2.91
3d 14.69±0.31 5.89±0.24 1.71±0.15 28..01±2.23
5d 14.57±0.42 6.61±0.37 1.79±0.29 27.99±3.55
7d 14.21±0.22 6.33±0.55 1.51±0.13 29.39±1.01
9d 13.25±0.21 5.51±0.31 1.27±0.07 33.95±2.34
12d 12.89±0.09 4.55±0.28 1.19±0.14 38.88±3.05
From table 2 it can be seen that the growing state of pepper seedling can be substantially improved by spraying (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution.
Embodiment described above is only to be described the preferred embodiment of the present invention, not design and the model to the present invention Enclose and be defined.On the premise of without departing from design concept of the present invention, this area ordinary person make technical scheme Various modification and improvement, all should drop into protection scope of the present invention, and the technology contents that the present invention is claimed the most all is recorded In detail in the claims.

Claims (10)

1. the application of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate, it is characterised in that (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate is applied to alleviate the waterflooding injury of shallow root system vegetable.
2. the application of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate described in claim 1, it is characterised in that described shallow root system vegetable is Solanaceae capsicum plants.
3. the application process of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate described in claim 1 or 2, it is characterised in that shallow during waterflooding or after waterflooding Root system vegetable foliage-spray (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution.
The application process of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate the most according to claim 3, it is characterised in that described (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution dense Degree is 0.3-2.0mmol L-1
The application process of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate the most according to claim 3, it is characterised in that described (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution dense Degree is 0.6-1.0mmol L-1
6. according to the application process of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate described in claim 4 or 5, it is characterised in that spray (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate continuously Solution 1-12 days.
7. according to the application process of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate described in claim 4 or 5, it is characterised in that spray (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate continuously Solution 3-7 days.
The application process of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate the most according to claim 3, it is characterised in that described (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate solution is for making Add water after dissolving with the ethanol solution that volumetric concentration is 75-95% the aqueous solution obtained.
The application process of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate the most according to claim 3, it is characterised in that described in spray during, spray continuously Execute to shallow root system vegetable blade face to there being lower of liquid.
The application process of (-)-methyl cis-2-pent-2'-enyl-3-oxocyclopentylacetate the most according to claim 9, it is characterised in that during waterflooding and waterflooding after to planting The blade of strain disease leaf and the jaundice of more than 50% area is extractd, and carries out loosing soil.
CN201610317177.3A 2016-05-13 2016-05-13 Application of the methyl jasmonate on plant is waterlogging-resistant Active CN105961390B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108184990A (en) * 2018-02-09 2018-06-22 江苏沿海地区农业科学研究所 A kind of method for extending refrigeration cucumber freshness date using methyl jasmonate
CN108353678A (en) * 2018-04-23 2018-08-03 天津农学院 A method of improving aloe oxidation resistance with methyl jasmonate
CN111903419A (en) * 2019-12-27 2020-11-10 河南科技大学 Method for improving drought resistance of oil peony by adopting methyl jasmonate
CN111903420A (en) * 2019-12-27 2020-11-10 河南科技大学 Method for improving drought resistance of oil peony by using jasmonic acid

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CN1379981A (en) * 2002-04-23 2002-11-20 四川龙蟒福生科技有限责任公司 Plant growth regulator composition with cold-resisting action
CN102665408A (en) * 2009-11-27 2012-09-12 先正达参股股份有限公司 Plant growth regulation

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108184990A (en) * 2018-02-09 2018-06-22 江苏沿海地区农业科学研究所 A kind of method for extending refrigeration cucumber freshness date using methyl jasmonate
CN108353678A (en) * 2018-04-23 2018-08-03 天津农学院 A method of improving aloe oxidation resistance with methyl jasmonate
CN111903419A (en) * 2019-12-27 2020-11-10 河南科技大学 Method for improving drought resistance of oil peony by adopting methyl jasmonate
CN111903420A (en) * 2019-12-27 2020-11-10 河南科技大学 Method for improving drought resistance of oil peony by using jasmonic acid

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