CN111685125A - Plant growth regulator and application thereof - Google Patents
Plant growth regulator and application thereof Download PDFInfo
- Publication number
- CN111685125A CN111685125A CN201910189079.XA CN201910189079A CN111685125A CN 111685125 A CN111685125 A CN 111685125A CN 201910189079 A CN201910189079 A CN 201910189079A CN 111685125 A CN111685125 A CN 111685125A
- Authority
- CN
- China
- Prior art keywords
- plant
- growth regulator
- plant growth
- cereal
- seeds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000005648 plant growth regulator Substances 0.000 title claims abstract description 49
- 239000007864 aqueous solution Substances 0.000 claims abstract description 18
- 241000196324 Embryophyta Species 0.000 claims description 45
- 235000007164 Oryza sativa Nutrition 0.000 claims description 34
- 235000009566 rice Nutrition 0.000 claims description 34
- 238000005507 spraying Methods 0.000 claims description 13
- 150000003839 salts Chemical class 0.000 claims description 12
- 238000002791 soaking Methods 0.000 claims description 11
- 239000003513 alkali Substances 0.000 claims description 9
- 240000008042 Zea mays Species 0.000 claims description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 8
- 230000007480 spreading Effects 0.000 claims description 7
- 238000003892 spreading Methods 0.000 claims description 7
- 239000011248 coating agent Substances 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 2
- 230000024346 drought recovery Effects 0.000 claims description 2
- 238000003973 irrigation Methods 0.000 claims description 2
- 230000002262 irrigation Effects 0.000 claims description 2
- 235000009973 maize Nutrition 0.000 claims description 2
- 239000002689 soil Substances 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 230000012010 growth Effects 0.000 abstract description 9
- 239000001963 growth medium Substances 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 34
- 241000209094 Oryza Species 0.000 description 33
- 239000000243 solution Substances 0.000 description 24
- 239000003795 chemical substances by application Substances 0.000 description 20
- 230000035882 stress Effects 0.000 description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 13
- 239000012153 distilled water Substances 0.000 description 13
- 239000008367 deionised water Substances 0.000 description 9
- 229910021641 deionized water Inorganic materials 0.000 description 9
- 230000035784 germination Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 6
- 235000005822 corn Nutrition 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000005708 Sodium hypochlorite Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000012452 mother liquor Substances 0.000 description 5
- 239000010413 mother solution Substances 0.000 description 5
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- 238000007865 diluting Methods 0.000 description 4
- 230000008641 drought stress Effects 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000008118 PEG 6000 Substances 0.000 description 2
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 241000187747 Streptomyces Species 0.000 description 2
- 230000000249 desinfective effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- 239000002270 dispersing agent Substances 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 238000010898 silica gel chromatography Methods 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- 229920005552 sodium lignosulfonate Polymers 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 239000000080 wetting agent Substances 0.000 description 2
- 239000000230 xanthan gum Substances 0.000 description 2
- 229920001285 xanthan gum Polymers 0.000 description 2
- 229940082509 xanthan gum Drugs 0.000 description 2
- 235000010493 xanthan gum Nutrition 0.000 description 2
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 238000005160 1H NMR spectroscopy Methods 0.000 description 1
- UZSSGAOAYPICBZ-SOCHQFKDSA-N Decoyinine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@]1(CO)OC(=C)[C@@H](O)[C@H]1O UZSSGAOAYPICBZ-SOCHQFKDSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000187180 Streptomyces sp. Species 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- UZSSGAOAYPICBZ-UHFFFAOYSA-N angustmycin A Natural products C1=NC=2C(N)=NC=NC=2N1C1(CO)OC(=C)C(O)C1O UZSSGAOAYPICBZ-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000015784 hyperosmotic salinity response Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 231100001083 no cytotoxicity Toxicity 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
A plant growth regulator and application thereof, belonging to the technical field of agricultural science. In order to improve the stress resistance of crops, the invention provides a plant growth regulator, which is an aqueous solution containing the five-cereal-element, wherein the five-cereal-element is used as an effective component, the concentration in the plant growth regulator is 0.5mg/L-200mg/L, different application modes and concentrations are adopted according to different growth stages of plant plants, and the plant growth regulator can be used for seeds, whole plants, parts of crops or culture media of the crops, so that the stress resistance of the crops is obviously enhanced, and the growth of the plants is promoted.
Description
Technical Field
The invention belongs to the technical field of agricultural science, and particularly relates to a plant growth regulator and application thereof.
Background
In crop and vegetable production, abiotic stresses such as drought, saline alkali, extreme temperature and the like are frequently encountered, the normal growth and development of plants are seriously affected, and finally the death of the plants and the reduction of the yield are caused. On the basis of improvement of plant varieties, technologies and planting modes, the beneficial exogenous plant growth regulator has important economic significance for improving the stress resistance of plants. The wugufengin (also known as decoyinine) and angumycin a (angustmycina) is easily soluble in water, has good thermal stability and almost no cytotoxicity. The mice are injected with 2.5g/kg in the abdominal cavity without any toxic and side effects.
Disclosure of Invention
In order to improve the stress resistance of crops, the invention provides a plant growth regulator, which is an aqueous solution containing the five-cereal-element, wherein the five-cereal-element is used as an effective component, and the concentration of the five-cereal-element in the plant growth regulator is 0.5mg/L-200 mg/L.
Preferably, the concentration of the five-cereal plumping agent in the plant growth regulator is 30mg/L-100 mg/L.
More preferably, the concentration of the pentaglutenin in the plant growth regulator is 50 mg/L.
The invention also provides application of the plant growth regulator in improving the stress resistance of plants.
Further, it is defined that seeds, whole plants, tissue parts of plants or culture media of the plants are treated with the above plant growth regulator.
Further defined, the treatment is a spreading treatment to the plant or plant seed or a spreading treatment to soil surrounding the plant or an irrigation treatment.
Further defined, the spreading treatment of the plant is spraying to the foliage of the plant; the spreading treatment of the plant seeds refers to seed soaking or coating treatment of the plant seeds by using the plant growth regulator.
Further defined, the spraying is performed before or during the stress of the plant.
Further defined, the application refers to the application of the plant growth regulator in improving stress resistance of wheat, rice, corn or soybean.
Further defined, the stress tolerance includes cold tolerance, salt tolerance, alkali tolerance and drought tolerance.
Advantageous effects
The invention aims to research a use method for improving the stress resistance of crops by using the five-cereal plumping agent, relieve the damages of drought, salt and alkali and extreme temperature of the plants, promote the growth and development of the plants and improve the yield of the crops and vegetables.
The application concentration of the plant growth regulator containing the five-cereal plumping element is 0.5-200 mg/L, the preferred concentration is 30-100 mg/L, different application modes and concentrations are adopted according to different growth stages of plant plants, and the plant growth regulator can be used for seeds of plants (or crops), whole plants of the plants, plant parts or culture media of the plants.
Drawings
FIG. 1 shows the structure of five-cereal plumes.
Detailed Description
The present invention will be described in further detail with reference to examples, but the present invention is not limited thereto.
The five-cereal plumping agent can be prepared and obtained by a microbial synthesis way: streptomyces NEAU6 is fermented and cultured, and the culture solution is extracted to prepare the compound of the oryzanol. The Streptomyces (Streptomyces sp.) NEAU6 is preserved in China general microbiological culture Collection center at 24.08.2018, and the preservation addresses are as follows: no. 3 of Xilu No. 1 of Beijing, upto, is CGMCC 16338.
The culture medium is as follows:
gao's first agar medium: 20g of soluble starch, 0.5g of NaCl and KNO31g，FeSO4.7H2O 0.01g，K2HPO40.5g，MgSO4.7H20.5g of O, 20g of agar and 1000ml of distilled water, and the pH value is 7.2-7.4.
Seed culture medium: tryptone 17g, soybean peptone 3g, NaCl 5g, K2HPO42.5g, 2.5g of glucose and 1000ml of distilled water, and the pH value is 7.2-7.4.
Fermentation medium: 10g of soluble starch, 10g of glucose, 10g of glycerol, 5g of tryptone, 5g of yeast powder and CaCO33g, 1000ml of tap water and pH 7.2-7.4.
Under aseptic conditions, the spores of the strain NEAU6 which had been activated on the Gao's first agar medium were scraped off and transferred to a seed medium, cultured at 30 ℃ and 200rpm for 2 days, and then the seed solution was inoculated at 6% (v/v) to a fermentation medium, and cultured at 30 ℃ and 200rpm for 5 days. Centrifuging to remove thallus, extracting the supernatant with ethyl acetate of the same volume for 3-4 times, concentrating the extractive solution, and sequentially performing silica gel column chromatography, gel chromatography, and C-18 column chromatography to obtain the compound. The structure of the compound is determined by 1H-NMR, 13C-NMR and MS spectrum analysis, and the structural formula is shown in figure 1.
The related plant varieties, the film-forming agent polyvinyl alcohol, the dispersant sodium lignosulfonate, the thickener xanthan gum, the wetting agent fatty acid, the experimental ware and the like can be purchased and obtained through commercial approaches.
Example 1 application of a plant growth regulator to improve drought resistance of rice.
The plant growth regulator in the embodiment is an aqueous solution containing the five-cereal-element, and the concentration of the five-cereal-element in the plant growth regulator is 0.5mg/L-200 mg/L.
The application of the plant growth regulator in improving the drought resistance of rice refers to soaking rice seeds in 0.5-200 mg/L of a pentaglutenin aqueous solution at room temperature for 12-24 hours.
The method comprises the following specific steps:
(1) weighing 50mg of the five-cereal plumping agent, dissolving with deionized water, and fixing the volume to 100mL to prepare 500mg/L five-cereal plumping agent mother solution;
(2) taking different volumes of the mother liquor in the step (1), adding deionized water according to a proportion, and diluting into 0.5-200 mg/L of five-cereal-element water solutions with different concentrations, wherein the concentrations are shown in Table 1;
(3) selecting rice Longjing 31 seeds with uniformly filled seeds, disinfecting for 3min by sodium hypochlorite with the volume percentage concentration of 5%, washing for 3 times by distilled water, and soaking the seeds for 48h by using five-cereal-feng-element water solutions with different concentration gradients.
(4) The method comprises the steps of carrying out drought stress germination test on rice variety Longjing 31 seeds, transferring the soaked seeds into culture dishes with the diameter of 9cm, and repeating the process for three times for 16 rice seeds in each culture dish. And then adding 10 percent of PEG-6000 solution by mass percent into each culture dish for simulating drought stress treatment, replacing the PEG-6000 solution once every 12h, observing the germination condition of the rice seeds after 4 days, and counting the root length and the bud length of the rice. The results are shown in Table 1.
Table 1. influence of soaking seeds in water solutions of five-cereal plumping elements with different concentrations on germination of rice Longjing 31 seeds under drought stress
As can be seen from Table 1, the water solutions of oryzanol with different concentrations can improve the drought resistance of rice seeds. From the comparison of germination rate, root length and bud length, it can be seen that the drought stress of rice seeds can be relieved by soaking the seeds with the five-cereal plumping agent, the germination growth of the rice seeds is obviously improved, and the bud length and the root length are respectively improved by 34.9 percent and 65.4 percent at the optimum concentration of 50mg/L compared with the control.
Example 2 application of plant growth regulator in improving salt stress ability of rice.
The plant growth regulator in the embodiment is an aqueous solution containing the five-cereal-element, and the concentration of the five-cereal-element in the plant growth regulator is 0.5mg/L-200 mg/L.
The application of the plant growth regulator in improving the salt stress capability of rice refers to root soaking treatment by using 0.5-200 mg/L of a pentaglutenin aqueous solution at room temperature.
The method comprises the following specific steps:
(1) weighing 50mg of the five-cereal plumping agent, dissolving with deionized water, and fixing the volume to 100mL to prepare 500mg/L five-cereal plumping agent mother solution;
(2) taking different volumes of the mother liquor in the step (1), adding deionized water according to a proportion, and diluting into 0.5-200 mg/L of five-cereal-element water solutions with different concentrations, wherein the concentrations are shown in Table 2;
(3) selecting rice variety Longjing 31 seeds with uniform full seeds, sterilizing with sodium hypochlorite with volume percentage concentration of 5% for 3min, washing with distilled water for 3 times, and soaking with distilled water.
(4) And (3) selecting uniform seeds, putting the seeds into a culture dish with the diameter of 9cm, culturing the seeds with distilled water, and culturing the seeds with a nutrient solution until the seeds grow to one leaf and one heart. Then soaking roots in the five-cereal-element water solution with different concentration gradients for 24 hours, then adding 0.2mol/L sodium chloride solution for stress treatment, replacing the sodium chloride solution every 24 hours, and measuring the plant height and the fresh root weight of the rice after 3 days. The results are shown in Table 2.
Table 2. influence of aqueous solutions of five-cereal Feng essence with different concentrations on growth of rice at Longjing 31 seedling stage under salt stress
As can be seen from Table 2, after the roots of the rice are soaked with the oryzanol with different concentrations in the seedling stage, the salt stress is carried out to enhance the salt resistance of the rice. When the concentration of the five-cereal plumping agent is 50mg/L, the effect is optimal, and compared with a control, the plant height, the seedling fresh weight and the root fresh weight are respectively improved by 42.5 percent, 31.6 percent and 19.6 percent.
Example 3 application of plant growth regulator in improving salt resistance of rice.
The plant growth regulator in the embodiment is an aqueous solution containing the five-cereal-element, and the concentration of the five-cereal-element in the plant growth regulator is 0.5mg/L-200 mg/L.
The plant growth regulator is applied to improving the salt resistance of rice, and specifically, 0.5-200 mg/L of a penta-oryzanol aqueous solution is sprayed on leaves at room temperature, and the spraying amount is 0.1L/m2Spraying for 2-3 times.
The method comprises the following specific steps:
(1) weighing 50mg of the five-cereal plumping agent, dissolving with deionized water, and fixing the volume to 100mL to prepare 500mg/L five-cereal plumping agent mother solution;
(2) taking different volumes of the mother liquor in the step (1), adding deionized water according to a proportion, and diluting into 0.5-200 mg/L of five-cereal-element water solutions with different concentrations, wherein each concentration is shown in Table 3;
(3) selecting rice variety Longjing 31 seeds with uniform full seeds, sterilizing with sodium hypochlorite with volume percentage concentration of 5% for 3min, washing with distilled water for 3 times, and soaking with distilled water.
(4) And (3) selecting uniform seeds, putting the seeds into a culture dish with the diameter of 9cm, culturing the seeds with distilled water, and culturing the seeds with a nutrient solution until the seeds grow to one leaf and one heart. And then spraying with the aqueous solution of the oryzanol with different concentration gradients once every 12 hours for 3 times in total, then adding 0.2mol/L sodium chloride solution for stress treatment, replacing the sodium chloride solution once every 24 hours, and measuring the plant height, the plant height and the fresh weight of the rice after 3 days. The results are shown in Table 3.
Table 3. influence of spraying of aqueous solutions of oryzanol with different concentrations on growth of rice at Longjing 31 seedling stage under salt stress
As can be seen from Table 3, after the rice is sprayed with the aqueous solutions of the oryzanol with different concentrations in the seedling stage, the salt stress is carried out to enhance the salt resistance of the rice. When the concentration of the five-cereal plumping agent is 50mg/L, the effect is optimal, and compared with a control, the plant height, the seedling fresh weight and the root fresh weight are respectively improved by 46.1 percent, 27.7 percent and 19 percent.
Example 4 application of plant growth regulator in improving the alkali resistance of rice.
The plant growth regulator in the embodiment is an aqueous solution containing the five-cereal-element, and the concentration of the five-cereal-element in the plant growth regulator is 0.5mg/L-200 mg/L.
The plant growth regulator described in the embodiment is applied to improving the alkali resistance of rice, and specifically, 0.5mg/L-200mg/L of a penta-oryzanol aqueous solution is used for foliage spraying at room temperature, and the spraying amount is 0.1L/m2Spraying for 2-3 times.
The method comprises the following specific steps:
(1) weighing 50mg of the five-cereal plumping agent, dissolving with deionized water, and fixing the volume to 100mL to prepare 500mg/L five-cereal plumping agent mother solution;
(2) taking different volumes of the mother liquor in the step (1), adding deionized water according to a proportion, and diluting into 0.5-200 mg/L of five-cereal-element water solutions with different concentrations, wherein the concentrations are shown in Table 4;
(3) selecting rice variety Longjing 31 seeds with uniform full seeds, sterilizing with sodium hypochlorite with volume percentage concentration of 5% for 3min, washing with distilled water for 3 times, and soaking with distilled water.
(4) And (3) selecting uniform seeds, putting the seeds into a culture dish with the diameter of 9cm, culturing the seeds with distilled water, and culturing the seeds with a nutrient solution until the seeds grow to one leaf and one heart. And then spraying with the aqueous solution of the oryzanol with different concentration gradients once every 12 hours for 3 times, then adding 0.2mol/L sodium carbonate solution for stress treatment, replacing the sodium carbonate solution once every 24 hours, and measuring the plant height, plant height and fresh weight of the rice after 3 days. The results are shown in Table 4.
TABLE 4 influence of water solutions of oryzanol of different concentrations on the growth of rice at the Longjing 31 seedling stage under alkali stress
As can be seen from Table 4, the alkali resistance of rice can be enhanced by spraying the water solutions of the oryzanol with different concentrations in the seedling stage and then carrying out alkali stress. When the concentration of the five-cereal plumping agent is 50mg/L, the effect is optimal, and compared with a control, the plant height, the seedling fresh weight and the root fresh weight are respectively improved by 13.1 percent, 33 percent and 30.1 percent.
Example 5 application of plant growth regulator to improve cold resistance of corn.
The plant growth regulator in the embodiment is an aqueous solution containing the five-cereal-element, and the concentration of the five-cereal-element in the plant growth regulator is 0.5mg/L-200 mg/L.
The plant growth regulator is applied to improving the cold resistance of corn, and specifically, the seeds are coated with 0.5mg/L-200mg/L of a pentaglutenin aqueous solution at room temperature.
The method comprises the following specific steps:
(1) weighing 50mg of the five-cereal plumping agent, dissolving with deionized water, and fixing the volume to 100mL to prepare 500mg/L five-cereal plumping agent mother solution;
(2) taking the mother liquor in the step (1) with different volumes, 5% of film-forming agent polyvinyl alcohol, 2% of dispersing agent sodium lignosulfonate, 1% of thickening agent xanthan gum, 1% of wetting agent fatty acid and the balance of water, and preparing the oryzanol seed coating agent with different concentrations of 0.5-200 mg/L, wherein each concentration is shown in Table 5;
(3) selecting seeds of a corn variety No. 1 Demeia with plump and uniform size, disinfecting for 3min by sodium hypochlorite with the volume percentage concentration of 5%, washing for 3 times by distilled water, then dressing seeds by a five-cereal plumping seed coating agent, uniformly stirring the seed coating agent and airing for later use;
(4) before sowing, selecting corn seeds with complete seed coats, consistent sizes and full seeds, sowing the seeds in a plastic box with a layer of wet sand spread at the bottom, sowing 10 seeds in each pot, covering the wet sand by about 1cm, putting the pot in an illumination incubator for cultivation, setting the temperature to be 15 ℃, and measuring the germination percentage after 7 days.
TABLE 5 Effect of varying concentrations of the five-cereal Fender seed coating on germination of maize variety No. 1 Germany seed under Low temperature stress
The results show that the low-temperature resistance of the corn seeds can be improved by the aid of the five-cereal plumping agents with different concentrations. When the concentrations of the five-grain Feng essence are respectively 0.5mg/L, 10mg/L, 30mg/L, 50mg/L, 100mg/L and 200mg/L, the germination rates are respectively 23%, 43%, 61%, 72%, 70% and 37%, and are obviously higher than the 20% germination rate of the comparison group.
Claims (10)
1. A plant growth regulator is characterized in that the plant growth regulator is an aqueous solution containing the five-cereal-element, wherein the five-cereal-element is taken as an effective component, and the concentration of the five-cereal-element in the plant growth regulator is 0.5mg/L-200 mg/L.
2. The plant growth regulator according to claim 1, wherein the concentration of the pentaglutenin in the plant growth regulator is 30mg/L to 100 mg/L.
3. The plant growth regulator according to claim 1, wherein the concentration of the pentaglutenin in the plant growth regulator is 50 mg/L.
4. Use of a plant growth regulator according to any one of claims 1 to 3 for improving stress tolerance in plants.
5. Use according to claim 4, characterized in that the seeds, the whole plants, the tissue parts of a plant or the cultivation medium of the plant are treated with a plant growth regulator according to any one of claims 1 to 3.
6. Use according to claim 5, wherein the treatment is a spreading treatment to the plant or plant seed or a spreading treatment to the soil surrounding the plant or an irrigation treatment.
7. The use according to claim 6, wherein the plant is spread by spraying onto the foliage of the plant; the spreading treatment of the plant seeds refers to seed soaking or coating treatment of the plant seeds by using the plant growth regulator.
8. The use according to claim 7, wherein the spraying is performed before or during stress of the plant.
9. The use of claim 4, wherein the use is of a plant growth regulator for improving stress tolerance in wheat, rice, maize or soybean.
10. The use according to claim 4, wherein the stress tolerance comprises cold, salt, alkali and drought tolerance.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910189079.XA CN111685125A (en) | 2019-03-13 | 2019-03-13 | Plant growth regulator and application thereof |
| JP2021552924A JP7454587B2 (en) | 2019-03-13 | 2019-07-03 | Plant growth regulators and their applications |
| PCT/CN2019/094501 WO2020181694A1 (en) | 2019-03-13 | 2019-07-03 | Plant growth regulator and application thereof |
| JP2024036414A JP2024069371A (en) | 2019-03-13 | 2024-03-08 | Plant growth regulators and their applications |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910189079.XA CN111685125A (en) | 2019-03-13 | 2019-03-13 | Plant growth regulator and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111685125A true CN111685125A (en) | 2020-09-22 |
Family
ID=72426844
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910189079.XA Pending CN111685125A (en) | 2019-03-13 | 2019-03-13 | Plant growth regulator and application thereof |
Country Status (3)
| Country | Link |
|---|---|
| JP (2) | JP7454587B2 (en) |
| CN (1) | CN111685125A (en) |
| WO (1) | WO2020181694A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113207470B (en) * | 2021-06-04 | 2023-09-08 | 南京农业大学 | Argon-based liquid growth regulator for plants and edible fungi and preparation method and application thereof |
| CN114532338B (en) * | 2022-02-16 | 2023-08-22 | 贵州大学 | Rice culture solution containing gamma-aminobutyric acid, application of rice culture solution in nitrogen stress resistance of rice and rice culture method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4225585A (en) * | 1978-05-23 | 1980-09-30 | Ajinomoto Company, Incorporated | Fungicidal and bactericidal compositions and method for protecting plants by use thereof |
| CN1436462A (en) * | 2002-12-27 | 2003-08-20 | 广西大学 | Phytological and agronomic application of angustmycin |
| CN101218896A (en) * | 2008-01-24 | 2008-07-16 | 广西大学 | Method for producing momordica grosvenori group seedling with single culture medium |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109694838B (en) * | 2019-03-13 | 2022-03-04 | 东北农业大学 | Streptomyces and application thereof |
-
2019
- 2019-03-13 CN CN201910189079.XA patent/CN111685125A/en active Pending
- 2019-07-03 WO PCT/CN2019/094501 patent/WO2020181694A1/en active Application Filing
- 2019-07-03 JP JP2021552924A patent/JP7454587B2/en active Active
-
2024
- 2024-03-08 JP JP2024036414A patent/JP2024069371A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4225585A (en) * | 1978-05-23 | 1980-09-30 | Ajinomoto Company, Incorporated | Fungicidal and bactericidal compositions and method for protecting plants by use thereof |
| CN1436462A (en) * | 2002-12-27 | 2003-08-20 | 广西大学 | Phytological and agronomic application of angustmycin |
| CN101218896A (en) * | 2008-01-24 | 2008-07-16 | 广西大学 | Method for producing momordica grosvenori group seedling with single culture medium |
Non-Patent Citations (6)
| Title |
|---|
| 卢少云等: "草坪草逆境生理研究进展 ", 《草业学报》 * |
| 周岐伟等: "PGR－08对小麦幼苗抗旱性的影响 ", 《广西农业生物科学》 * |
| 王三根: "细胞分裂素在植物抗逆和延衰中的作用 ", 《植物学通报》 * |
| 许鸿源: "PGR-08生物活性及其应用研究 ", 《广西农业生物科学》 * |
| 许鸿源等: "PGR-08理化性质及生物活性的研究 ", 《广西植物》 * |
| 韩征等: "狭霉素及其应用研究的新进展", 《中国抗生素杂志》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022523241A (en) | 2022-04-21 |
| JP7454587B2 (en) | 2024-03-22 |
| WO2020181694A1 (en) | 2020-09-17 |
| JP2024069371A (en) | 2024-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104380994A (en) | Bletilla striata large-scale high-yield cultivation method | |
| CN105594448A (en) | Artificial seedling rearing method of cucumber seeds | |
| CN107087641B (en) | Marine oligosaccharide biological preparation for improving salt resistance of crops and preparation method thereof | |
| CN105993458A (en) | Planting method for pea | |
| JP2024069371A (en) | Plant growth regulators and their applications | |
| CN105994379B (en) | Coating agent for seed based on actinomyces and preparation method thereof and coating method | |
| CN105993956A (en) | Fast propagating method for atractylis lancea | |
| CN106282067A (en) | Multifunctional agricultural complex micro organism fungicide and microbial ecological agent and application | |
| CN105309255A (en) | A rice planting method | |
| CN103131641A (en) | T.hariamum multivariate fermentation method and application | |
| CN113079741A (en) | Rapid seedling growing method for polygonatum cyrtonema | |
| CN110856469A (en) | Cultivation method for direct seeding and half-period dry farming of rice | |
| CN104737668A (en) | Wild soybean seeds and method for increasing germination rate and survival rate thereof | |
| CN105340708A (en) | Industrialized seedling production method of panax notoginseng | |
| CN112119913A (en) | Virus-free propagation method for sweet potatoes | |
| CN106550623A (en) | A kind of high-quality method for culturing seedlings of astragalus membranaceus seed | |
| CN104230404A (en) | Preparation method of enzymatic hydrolysis algae organic fertilizer and product thereof | |
| CN110199804A (en) | A kind of fragrant high yield cultivating method of the excellent guarantor of rice guarantor | |
| CN110199753A (en) | A kind of nuisanceless Chinese prickly ash implantation methods of green ecological | |
| CN104938194A (en) | High-yield and high-quality pea planting method | |
| CN108739403A (en) | A kind of tissue culture and rapid propagation method of rose wood | |
| CN104969787B (en) | A kind of method for improving purslane seed production | |
| CN103931452A (en) | Filbert high-yield cultivation method | |
| CN112624840A (en) | Biological pesticide fertilizer containing bacillus methylotrophicus | |
| CN105993871A (en) | Rapid seedling-raising method capable of promoting high germination rate of pseudo-ginseng by utilizing acremonium strictum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200922 |
|
| RJ01 | Rejection of invention patent application after publication |