JP7454587B2 - Plant growth regulators and their applications - Google Patents
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- JP7454587B2 JP7454587B2 JP2021552924A JP2021552924A JP7454587B2 JP 7454587 B2 JP7454587 B2 JP 7454587B2 JP 2021552924 A JP2021552924 A JP 2021552924A JP 2021552924 A JP2021552924 A JP 2021552924A JP 7454587 B2 JP7454587 B2 JP 7454587B2
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- 235000007164 Oryza sativa Nutrition 0.000 claims description 30
- 235000009566 rice Nutrition 0.000 claims description 30
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- 238000000034 method Methods 0.000 claims description 17
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- 238000002791 soaking Methods 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
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- 230000012010 growth Effects 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
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- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 2
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
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- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
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- UZSSGAOAYPICBZ-SOCHQFKDSA-N Decoyinine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@]1(CO)OC(=C)[C@@H](O)[C@H]1O UZSSGAOAYPICBZ-SOCHQFKDSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
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- 241000187747 Streptomyces Species 0.000 description 1
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- 230000036579 abiotic stress Effects 0.000 description 1
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- UZSSGAOAYPICBZ-UHFFFAOYSA-N angustmycin A Natural products C1=NC=2C(N)=NC=NC=2N1C1(CO)OC(=C)C(O)C1O UZSSGAOAYPICBZ-UHFFFAOYSA-N 0.000 description 1
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- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 description 1
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- 238000000855 fermentation Methods 0.000 description 1
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- 235000011187 glycerol Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920005552 sodium lignosulfonate Polymers 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Description
本発明は、農業科学技術の分野に属し、特に植物成長調整剤及びその応用に関する。 The present invention belongs to the field of agricultural science and technology, and in particular relates to plant growth regulators and their applications.
作物や野菜の生産中に、干ばつ、塩分、極端な温度などの非生物的ストレスに遭遇することが多く、これは植物の正常な成長と発育に深刻な影響を及ぼし、最終的には植物の死と収量の低下を引き起こす。植物の品種、技術、植栽パターンの改善に基づいて、有益な外因性植物成長調整剤の使用は、植物のストレス耐性を改善するための重要な経済的意義を持っている。デコイニン（ｄｅｃｏｙｉｎｉｎｅ）およびアンガストマイシンＡ（ａｎｇｕｓｔｍｙｃｉｎ Ａ）としても知られる五穀豊（ｗｕｇｕｆｅｎｇｉｎ）は、水に溶けやすく、熱安定性が高く、細胞毒性がほとんどない。マウスの腹腔内へ２．５ｇ／ｋｇを注射すると、いかなる中毒性副反応がない。 During the production of crops and vegetables, abiotic stresses such as drought, salinity, and extreme temperatures are often encountered, which seriously affects the normal growth and development of plants and ultimately Causes death and reduced yield. Based on improvements in plant varieties, techniques, and planting patterns, the use of beneficial exogenous plant growth regulators has important economic implications for improving plant stress tolerance. Wugufengin, also known as decoyinine and angustomycin A, is easily soluble in water, highly thermostable, and has little cytotoxicity. Injecting 2.5 g/kg intraperitoneally into mice does not cause any toxic side effects.
本発明は、農作物のストレス耐性を改善するために、植物成長調整剤を提供する。 The present invention provides plant growth regulators for improving stress tolerance of agricultural crops.
前記植物成長調整剤は、五穀豊を含む水溶液であり、有効成分としての五穀豊は、植物成長調整剤での濃度が０．５ｍｇ／Ｌ～２００ｍｇ／Ｌである。 The plant growth regulator is an aqueous solution containing Gokokuho, and the concentration of Gokokuho as an active ingredient in the plant growth regulator is 0.5 mg/L to 200 mg/L.
好ましくは、植物成長調整剤中の前記五穀豊の濃度は３０ｍｇ／Ｌ～１００ｍｇ／Ｌである。 Preferably, the concentration of Gokokuho in the plant growth regulator is 30 mg/L to 100 mg/L.
より好ましくは、植物成長調整剤中の前記五穀豊の濃度は５０ｍｇ／Ｌである。 More preferably, the concentration of Gokokuho in the plant growth regulator is 50 mg/L.
本発明はまた、植物のストレス耐性を改善する際の前記植物成長調整剤の応用を提供する。 The present invention also provides the application of said plant growth regulator in improving stress tolerance of plants.
さらに限定的に、植物種子、植物全体、組織部分または該植物の培養培地を、上記の植物成長調整剤で処理する。 More specifically, plant seeds, whole plants, tissue parts or the culture medium of the plants are treated with the above-mentioned plant growth regulators.
さらに限定的に、前記処理は、植物または植物種子への散布処理、または植物の周囲の土壌への散布処理または灌漑処理である。 More specifically, the treatment is a spraying treatment on the plants or plant seeds, or a spraying treatment or irrigation treatment on the soil surrounding the plants.
さらに限定的に、前記植物の散布処理は、植物の葉面への噴霧を指し、前記植物種子の散布処理は、植物成長調節剤による植物種子の浸漬またはコーティング処理を指す。 More specifically, the spraying treatment of plants refers to spraying on the leaves of plants, and the spraying treatment of plant seeds refers to soaking or coating the plant seeds with the plant growth regulator.
さらに限定的に、前記噴霧のタイミングは、植物の逆境ストレスの前または逆境ストレス中である。 More specifically, the timing of said spraying is before or during adversity stress of the plant.
さらに限定的に、前記応用は、小麦、米、トウモロコシまたは大豆のストレス耐性を改善するための植物成長調製剤の応用を指す。 More specifically, said application refers to the application of plant growth regulators for improving the stress tolerance of wheat, rice, corn or soybean.
さらに限定的に、前記ストレス耐性には、耐寒性、耐塩性、耐アルカリ性、耐乾性が含まれる。 More specifically, the stress resistance includes cold resistance, salt resistance, alkali resistance, and drought resistance.
本発明は、五穀豊を使用して作物のストレス耐性を向上させる方法を利用して、植物の干ばつ、アルカリ性、および極端な温度による損傷を軽減し、植物の成長および発育を促進し、作物および野菜の収量を増加させることを研究することを目的とする。 The present invention utilizes the method of using gokufu to improve stress tolerance of crops to reduce drought, alkalinity, and extreme temperature damage of plants, promote plant growth and development, and improve crop and The aim is to study how to increase vegetable yield.
本発明の前記五穀豊を含む植物成長調整剤の施用濃度は、０．５ｍｇ／Ｌ～２００ｍｇ／Ｌであり、好ましくは３０ｍｇ／Ｌ～１００ｍｇ／Ｌである。植物の異なる成長段階に応じて、異なる施用方法および濃度を使用し、植物（または作物）の種子、植物全体、植物の部分、または該植物の培養培地において使用できる。本発明の方法は、実施が容易であり、操作が容易であり、五穀豊は有効成分として、作物の抵抗力を短期間で大幅に高めることができ、効果の持続期間が長く、それにより植物の健康的な成長を促進する。 The application concentration of the plant growth regulator containing the above-mentioned Gokokuho of the present invention is 0.5 mg/L to 200 mg/L, preferably 30 mg/L to 100 mg/L. Depending on the different growth stages of the plant, different application methods and concentrations are used and can be used in the seeds of the plant (or crop), the whole plant, parts of the plant, or the culture medium of the plant. The method of the present invention is easy to implement and easy to operate, and Gokokufu as an active ingredient can greatly increase the resistance of crops in a short period of time, has a long duration of effect, and thereby promote healthy growth.
以下では、実施例を参照しながら本発明をさらに詳細に説明するが、本発明の内容は限定されない。 Hereinafter, the present invention will be explained in more detail with reference to Examples, but the content of the present invention is not limited.
本発明の前記五穀豊は、微生物合成によって調製することができる。ストレプトマイセス属ＮＥＡＵ６を発酵培養し、培養液を抽出して化合物五穀豊を調製する。前記ストレプトミセス属（Ｓｔｒｅｐｔｏｍｙｃｅｓ ｓｐ．）ＮＥＡＵ６は、２０１８年８月２４日に中国微生物菌種保蔵管理委員会普通微生物センターに寄託され、寄託アドレスは北京市朝陽区北辰西路１号院３号、寄託番号はＣＧＭＣＣ １６３３８である。 The above-mentioned gokokuho of the present invention can be prepared by microbial synthesis. Streptomyces NEAU6 is fermented and cultured, and the culture solution is extracted to prepare the compound Gokokuho. The Streptomyces sp. NEAU6 was deposited at the Ordinary Microbiology Center of the China Microbial Species Conservation and Management Committee on August 24, 2018, and the deposit address is No. 3, Hospital No. 1, Beichen West Road, Chaoyang District, Beijing; The deposit number is CGMCC 16338.
関係する培地は次のとおりである。
高氏一号寒天培地：可溶性デンプン２０ｇ、ＮａＣｌ ０．５ｇ、ＫＮＯ3 １ｇ、ＦｅＳＯ4．７Ｈ2Ｏ ０．０１ｇ、Ｋ2ＨＰＯ4０．５ｇ、ＭｇＳＯ4．７Ｈ2Ｏ ０．５ｇ、寒天２０ｇ、蒸留水１０００ｍｌ、ｐＨ ７．２～７．４。
種子培地：トリプトン１７ｇ、大豆ペプトン３ｇ、ＮａＣｌ ５ｇ、Ｋ2ＨＰＯ4 ２．５ｇ、グルコース２．５ｇ、蒸留水１０００ｍｌ、ｐＨ ７．２～７．４。
発酵培地：可溶性デンプン１０ｇ、グルコース１０ｇ、グリセリン１０ｇ、トリプトン５ｇ、酵母エキス５ｇ、ＣａＣＯ3３ｇ、水道水１０００ｍｌ、ｐＨ ７．２～７．４。
The relevant media are as follows.
Takaji No. 1 agar medium: 20 g of soluble starch, 0.5 g of NaCl, 1 g of KNO 3 , FeSO 4 . 7H 2 O 0.01 g, K 2 HPO 4 0.5 g, MgSO 4 . 7H 2 O 0.5g, agar 20g, distilled water 1000ml, pH 7.2-7.4.
Seed medium: 17 g tryptone, 3 g soybean peptone, 5 g NaCl, 2.5 g K 2 HPO 4 , 2.5 g glucose, 1000 ml distilled water, pH 7.2-7.4.
Fermentation medium: 10 g soluble starch, 10 g glucose, 10 g glycerin, 5 g tryptone, 5 g yeast extract, 3 g CaCO 3 , 1000 ml tap water, pH 7.2-7.4.
無菌状態で、高氏一号寒天培地で活性化されたＮＥＡＵ６株の胞子をこすり取り、種子培地に移し、３０℃、２００ｒｐｍで２日間培養し、次に、種子液を６％（ｖ／ｖ）で発酵培地に接種し、３０℃、２００ｒｐｍで５日間培養する。遠心分離して菌体を除去し、上澄み液を等しい体積の酢酸エチルで３～４回抽出し、抽出液を濃縮した後、シリカゲルカラムクロマトグラフィー、ゲルクロマトグラフィー、Ｃ－１８カラムクロマトグラフィーを順番に行い、クロマトグラフィー後に該化合物は得られる。１Ｈ－ＮＭＲ、１３Ｃ－ＮＭＲ、およびＭＳスペクトル分析によって該化合物の構造を決定し、構造式を図１に示す。 Under sterile conditions, the spores of NEAU6 strain activated on Takaji No. 1 agar medium were scraped off, transferred to a seed medium, and cultured at 30°C and 200 rpm for 2 days. ) and cultured at 30°C and 200 rpm for 5 days. Cells were removed by centrifugation, and the supernatant was extracted 3 to 4 times with equal volumes of ethyl acetate. After concentrating the extract, silica gel column chromatography, gel chromatography, and C-18 column chromatography were performed in order. The compound is obtained after chromatography. The structure of the compound was determined by 1H-NMR, 13C-NMR, and MS spectral analysis, and the structural formula is shown in FIG.
関連する植物品種、膜形成剤ポリビニルアルコール、分散剤リグノスルホン酸ナトリウム、増粘剤キサンタンガム、湿潤剤脂肪酸、および実験器具等はすべて、商業化アプローチを通じて購入することができる。 Relevant plant varieties, film forming agent polyvinyl alcohol, dispersing agent sodium lignosulfonate, thickening agent xanthan gum, wetting agent fatty acids, and laboratory equipment etc. can all be purchased through commercialization approaches.
実施例１．イネの耐乾性の改善における植物成長調整剤の応用。 Example 1. Application of plant growth regulators in improving drought tolerance of rice.
本実施例の前記植物成長調整剤は、五穀豊を含む水溶液であり、前記五穀豊は、植物成長調整剤における濃度が０．５ｍｇ／Ｌ～２００ｍｇ／Ｌであった。 The plant growth regulator of this example is an aqueous solution containing Gokokuho, and the concentration of Gokokuho in the plant growth regulator was 0.5 mg/L to 200 mg/L.
イネの耐乾性を改善するための本実施例に記載の植物成長調整剤の応用は、イネの種子を濃度０．５ｍｇ／Ｌ～２００ｍｇ／Ｌの五穀豊水溶液に室温で浸漬することを指し、浸漬時間は１２～２４時間の範囲内であればよい。 The application of the plant growth regulator described in this example to improve the drought tolerance of rice refers to soaking rice seeds in a five-grain water solution with a concentration of 0.5 mg/L to 200 mg/L at room temperature, The immersion time may be within the range of 12 to 24 hours.
具体的なステップは以下のとおりであった。
（１）五穀豊５０ｍｇを秤量し、脱イオン水で溶解し、１００ｍＬに定容し、５００ｍｇ／Ｌの五穀豊母液を調製した。
（２）ステップ（１）の体積が異なる母液を取り、比率に応じて脱イオン水を加え、０．５ｍｇ／Ｌ～２００ｍｇ／Ｌ間の濃度の異なる五穀豊水溶液に希釈し、各濃度を表１に示した。
（３）実がよく入っている均一なイネ竜粳３１種子を選択し、体積パーセント濃度５％の次亜塩素酸ナトリウムで３分間消毒し、蒸留水で３回すすいだ後、濃度勾配が異なる五穀豊水溶液で種子を４８時間浸漬した。
（４）干ばつストレス下でのイネ品種竜粳３１種子の発芽実験を行った：浸漬した種子を直径９ｃｍのペトリ皿に移し、各ペトリ皿に１６個のイネの種子を入れ、３回繰り返した。次に、干ばつストレスをシミュレートするために各ペトリ皿に１０％質量百分率のＰＥＧ－６０００溶液を追加し、１２時間ごとにＰＥＧ－６０００溶液を交換し、４日後のイネ種子の発芽を観察し、イネの根の長さと芽の長さを統計した。結果は表１に示した。
The specific steps were as follows.
(1) 50 mg of Gokokuho was weighed, dissolved in deionized water, and the volume was adjusted to 100 mL to prepare 500 mg/L of Gokokuho mother liquor.
(2) Take the mother liquors of different volumes from step (1), add deionized water according to the ratio, dilute them to Gokokuho water solutions with different concentrations between 0.5 mg/L and 200 mg/L, and display each concentration. Shown in 1.
(3) Select 31 seeds of uniform rice longkei with good fruit content, disinfect with sodium hypochlorite with a volume percent concentration of 5% for 3 minutes, and rinse with distilled water three times, after which the concentration gradient is different. Seeds were soaked in Gokokuhosui solution for 48 hours.
(4) A germination experiment was conducted on 31 seeds of rice variety Longkei under drought stress: The soaked seeds were transferred to Petri dishes with a diameter of 9 cm, 16 rice seeds were placed in each Petri dish, and the experiment was repeated three times. . Then, we added 10% mass percentage PEG-6000 solution to each Petri dish to simulate drought stress, replaced the PEG-6000 solution every 12 hours, and observed the germination of rice seeds after 4 days. , the root length and shoot length of rice were statisticized. The results are shown in Table 1.
表１から、濃度が異なる五穀豊水溶液がすべてイネ種子の耐乾性を改善できることがわかった。発芽率、根の長さ、芽の長さの比較から、五穀豊を用いて種子を浸漬すると、イネ種子の干ばつストレスが軽減され、イネ種子の発芽と成長が大幅に改善され、５０ｍｇ／Ｌの濃度で最適であり、対照と比較して、芽の長さと根の長さはそれぞれ３４．９％と６５．４％増加したことがわかった。 From Table 1, it was found that all the five-grain enriched water solutions with different concentrations could improve the drought tolerance of rice seeds. Comparison of germination rate, root length, and bud length showed that soaking seeds with Gokokuho reduced the drought stress of rice seeds, significantly improved the germination and growth of rice seeds, and added 50 mg/L of rice seeds. It was found that bud length and root length were increased by 34.9% and 65.4%, respectively, compared to the control.
実施例２．イネの塩ストレスの改善における植物成長調整剤の応用。 Example 2. Application of plant growth regulators in ameliorating salt stress in rice.
本実施例の前記植物成長調整剤は、五穀豊を含む水溶液であり、前記五穀豊は、植物成長調整剤における濃度が０．５ｍｇ／Ｌ～２００ｍｇ／Ｌであった。 The plant growth regulator of this example is an aqueous solution containing Gokokuho, and the concentration of Gokokuho in the plant growth regulator was 0.5 mg/L to 200 mg/L.
イネの塩ストレス能力を改善するための本実施例に記載の植物成長調整剤の応用とは、室温で０．５ｍｇ／Ｌ～２００ｍｇ／Ｌの五穀豊水溶液を使用して根浸漬処理を行うことを指した。 The application of the plant growth regulator described in this example to improve the salt stress ability of rice is to perform root immersion treatment using a 0.5 mg/L to 200 mg/L Gokokuho water solution at room temperature. pointed to.
具体的なステップは以下のとおりであった。
（１）五穀豊５０ｍｇを秤量し、脱イオン水で溶解し、１００ｍＬに定容し、５００ｍｇ／Ｌの五穀豊母液を調製した。
（２）ステップ（１）の体積が異なる母液を取り、比率に応じて脱イオン水を加え、０．５ｍｇ／Ｌ～２００ｍｇ／Ｌ間の濃度の異なる五穀豊水溶液に希釈し、各濃度を表２に示した。
（３）実がよく入っている均一なイネ品種竜粳３１種子を選択し、体積パーセント濃度５％の次亜塩素酸ナトリウムで３分間消毒し、蒸留水で３回すすぎ、種子を蒸留水に浸漬した。
（４）均一な種子をピックアップし、直径９ｃｍのペトリ皿に入れ、蒸留水で培養し、苗が一葉一芯に成長したら、栄養液を使って二葉一芯に培養した。次に、異なる濃度勾配を有する五穀豊水溶液を使用して、根を２４時間浸漬し、次に、０．２ｍｏｌ／Ｌ塩化ナトリウム溶液を追加してストレス処理を行い、２４時間ごとに塩化ナトリウム溶液を交換し、３日後にイネの高さと根の新鮮重量を測定した。結果は表２に示した。
The specific steps were as follows.
(1) 50 mg of Gokokuho was weighed, dissolved in deionized water, and the volume was adjusted to 100 mL to prepare 500 mg/L of Gokokuho mother liquor.
(2) Take the mother liquors of different volumes from step (1), add deionized water according to the ratio, dilute them to Gokokuho water solutions with different concentrations between 0.5 mg/L and 200 mg/L, and display each concentration. Shown in 2.
(3) Select 31 seeds of the rice cultivar Longkei with a good amount of fruit, disinfect them with sodium hypochlorite at a volume percent concentration of 5% for 3 minutes, rinse with distilled water three times, and soak the seeds in distilled water. Soaked.
(4) Uniform seeds were picked up, placed in a Petri dish with a diameter of 9 cm, and cultured in distilled water. When the seedlings grew to one leaf and one core, they were cultured to two leaves and one core using a nutrient solution. Next, the roots were immersed for 24 hours using Gokokuho water solutions with different concentration gradients, and then stress treatment was performed by adding 0.2 mol/L sodium chloride solution, and the sodium chloride solution was added every 24 hours. The height of the rice plants and the fresh weight of the roots were measured 3 days later. The results are shown in Table 2.
表２から、苗期で濃度が異なる五穀豊にイネの根を浸漬した後、塩ストレスを行うとイネの耐塩性を高めることができる。五穀豊濃度が５０ｍｇ／Ｌである場合、効果が最も高く、対照と比較して、高さ、苗木を含む新鮮重量、および根の新鮮重量は、それぞれ４２．５％、３１．６％、および１９．６％増加したことがわかった。 From Table 2, it is possible to increase the salt tolerance of rice by immersing the roots of rice in Gokokuho, which has different concentrations at the seedling stage, and then subjecting it to salt stress. When the grain enrichment concentration was 50 mg/L, the effect was the highest, and compared with the control, the height, fresh weight including seedlings, and fresh weight of roots were 42.5%, 31.6%, and 31.6%, respectively. It was found that the increase was 19.6%.
実施例３．イネの耐塩性の改善における植物成長調整剤の応用。 Example 3. Application of plant growth regulators in improving salt tolerance of rice.
本実施例の前記植物成長調整剤は、五穀豊を含む水溶液であり、前記五穀豊は、植物成長調整剤における濃度が０．５ｍｇ／Ｌ～２００ｍｇ／Ｌであった。 The plant growth regulator of this example is an aqueous solution containing Gokokuho, and the concentration of Gokokuho in the plant growth regulator was 0.5 mg/L to 200 mg/L.
イネの耐塩性を向上させるための本実施例に記載の植物成長調整剤の応用は、具体的には室温で０．５ｍｇ／Ｌ～２００ｍｇ／Ｌの五穀豊水溶液を使用して葉面に噴霧することであり、噴霧量は０．１Ｌ／ｍ2、２～３回噴霧した。 Specifically, the application of the plant growth regulator described in this example to improve the salt tolerance of rice involves spraying on the leaf surface using a 0.5 mg/L to 200 mg/L Gokokuho aqueous solution at room temperature. The spray amount was 0.1 L/m 2 and the spray was applied 2 to 3 times.
具体的なステップは以下のとおりであった。 The specific steps were as follows.
（１）五穀豊５０ｍｇを秤量し、脱イオン水で溶解し、１００ｍＬに定容し、５００ｍｇ／Ｌの五穀豊母液を調製した。
（２）ステップ（１）の体積が異なる母液を取り、比率に応じて脱イオン水を加え、０．５ｍｇ／Ｌ～２００ｍｇ／Ｌ間の濃度の異なる五穀豊水溶液に希釈し、各濃度を表３に示した。
（３）実がよく入っている均一なイネ品種竜粳３１種子を選択し、体積パーセント濃度５％の次亜塩素酸ナトリウムで３分間消毒し、蒸留水で３回すすぎ、種子を蒸留水に浸漬した。
（４）均一な種子をピックアップし、直径９ｃｍのペトリ皿に入れ、蒸留水で培養し、苗が一葉一芯に成長したら、栄養液を使って二葉一芯に培養した。次に、異なる濃度勾配を有する五穀豊水溶液を使用して、噴霧し、１２時間ごとに１回噴霧し、合計３回噴霧し、次に、０．２ｍｏｌ／Ｌ塩化ナトリウム溶液を追加してストレス処理を行い、２４時間ごとに塩化ナトリウム溶液を交換し、３日後にイネの高さと新鮮重量を測定した。結果は表３に示した。
(1) 50 mg of Gokokuho was weighed, dissolved in deionized water, and the volume was adjusted to 100 mL to prepare 500 mg/L of Gokokuho mother liquor.
(2) Take the mother liquors of different volumes from step (1), add deionized water according to the ratio, dilute them to Gokokuho water solutions with different concentrations between 0.5 mg/L and 200 mg/L, and display each concentration. Shown in 3.
(3) Select 31 seeds of the rice cultivar Longkei with a good amount of fruit, disinfect them with sodium hypochlorite at a volume percent concentration of 5% for 3 minutes, rinse with distilled water three times, and soak the seeds in distilled water. Soaked.
(4) Uniform seeds were picked up, placed in a Petri dish with a diameter of 9 cm, and cultured in distilled water. When the seedlings grew to one leaf and one core, they were cultured to two leaves and one core using a nutrient solution. Then, using Gokokufu water solution with different concentration gradients, spray, spray once every 12 hours, total 3 times, and then add 0.2 mol/L sodium chloride solution to stress The treatment was carried out, and the sodium chloride solution was changed every 24 hours, and the height and fresh weight of the rice plants were measured after 3 days. The results are shown in Table 3.
表３から、苗期でイネに濃度の異なる五穀豊水溶液を噴霧した後、塩ストレスを行うと、イネの耐塩性を高めることができる。五穀豊濃度が５０ｍｇ／Ｌである場合、効果が最も高く、対照と比較して、高さ、苗木を含む新鮮重量、および根の新鮮重量は、それぞれ４６．１％、２７．７％及び１９％増加したことがわかった。 From Table 3, it is possible to increase the salt tolerance of rice by spraying grain rich water solutions with different concentrations on rice at the seedling stage and then applying salt stress. When the grain enrichment concentration was 50 mg/L, the effect was the highest, and compared with the control, the height, fresh weight including seedlings, and fresh weight of roots were 46.1%, 27.7%, and 19%, respectively. % increase was found.
実施例４．イネの耐アルカリ性の改善における植物成長調整剤の応用。 Example 4. Application of plant growth regulators in improving alkaline tolerance of rice.
本実施例の前記植物成長調整剤は、五穀豊を含む水溶液であり、前記五穀豊は、植物成長調整剤における濃度が０．５ｍｇ／Ｌ～２００ｍｇ／Ｌであった。 The plant growth regulator of this example is an aqueous solution containing Gokokuho, and the concentration of Gokokuho in the plant growth regulator was 0.5 mg/L to 200 mg/L.
イネの耐アルカリ性を改善するための本実施例に記載の植物成長調整剤の応用は、具体的には室温で０．５ｍｇ／Ｌ～２００ｍｇ／Ｌの五穀豊水溶液を使用して葉面に噴霧することを指し、噴霧量は０．１Ｌ／ｍ2であり、２～３回噴霧した。 Specifically, the application of the plant growth regulator described in this example to improve the alkali tolerance of rice involves spraying on the leaf surface using a Gokokuho aqueous solution of 0.5 mg/L to 200 mg/L at room temperature. The spray amount was 0.1 L/m 2 and the spray was applied 2 to 3 times.
具体的なステップは以下のとおりであった。
（１）五穀豊５０ｍｇを秤量し、脱イオン水で溶解し、１００ｍＬに定容し、５００ｍｇ／Ｌの五穀豊母液を調製した。
（２）ステップ（１）の体積が異なる母液を取り、比率に応じて脱イオン水を加え、０．５ｍｇ／Ｌ～２００ｍｇ／Ｌ間の濃度の異なる五穀豊水溶液に希釈し、各濃度を表４に示した。
（３）実がよく入っている均一なイネ品種竜粳３１種子を選択し、体積パーセント濃度５％の次亜塩素酸ナトリウムで３分間消毒し、蒸留水で３回すすぎ、種子を蒸留水に浸漬した。
（４）均一な種子をピックアップし、直径９ｃｍのペトリ皿に入れ、蒸留水で培養し、苗が一葉一芯に成長したら、栄養液を使って二葉一芯に培養した。次に、異なる濃度勾配を有する五穀豊水溶液を使用して、噴霧し、１２時間ごとに１回噴霧し、合計３回噴霧し、次に、０．２ｍｏｌ／Ｌ炭酸ナトリウム溶液を追加してストレス処理を行い、２４時間ごとに炭酸ナトリウム溶液を交換し、３日後にイネの高さと新鮮重量を測定した。結果は表４に示した。
The specific steps were as follows.
(1) 50 mg of Gokokuho was weighed, dissolved in deionized water, and the volume was adjusted to 100 mL to prepare 500 mg/L Gokokuho mother liquor.
(2) Take the mother liquor of different volumes from step (1), add deionized water according to the ratio, dilute it to a water solution with different concentrations between 0.5 mg/L and 200 mg/L, and display each concentration. 4.
(3) Select 31 seeds of the rice cultivar Longcai with a good number of grains, disinfect them with sodium hypochlorite at a volume percent concentration of 5% for 3 minutes, rinse with distilled water three times, and soak the seeds in distilled water. Soaked.
(4) Uniform seeds were picked up, placed in a Petri dish with a diameter of 9 cm, and cultured in distilled water. When the seedlings grew to one leaf and one core, they were cultured to two leaves and one core using a nutrient solution. Then, using Gokokufu water solution with different concentration gradients, spray, spray once every 12 hours, a total of 3 times, and then add 0.2 mol/L sodium carbonate solution to stress The treatment was carried out, and the sodium carbonate solution was changed every 24 hours, and the height and fresh weight of the rice plants were measured after 3 days. The results are shown in Table 4.
表４から、苗期で濃度が異なる五穀豊水溶液をイネに噴霧した後、アルカリストレスを行うとイネの耐アルカリ性を高めることができる。五穀豊の濃度が５０ｍｇ／Ｌである場合、効果が最も高く、対照と比較して、高さ、苗木を含む新鮮重量、および根の新鮮重量は、それぞれ１３．１％、３３％及び３０．１％増加したことがわかった。 From Table 4, it is possible to improve the alkali tolerance of rice by spraying rice with a five-grain enrichment solution having different concentrations at the seedling stage and then subjecting it to alkaline stress. When the concentration of Gokokuho was 50 mg/L, the effect was the highest, and compared with the control, the height, fresh weight including seedlings, and fresh weight of roots were 13.1%, 33%, and 30%, respectively. It was found that there was an increase of 1%.
実施例５．トウモロコシの耐寒性の改善における植物成長調整剤の応用。 Example 5. Application of plant growth regulators in improving cold tolerance of maize.
本実施例の前記植物成長調整剤は、五穀豊を含む水溶液であり、前記五穀豊は、植物成長調整剤における濃度が０．５ｍｇ／Ｌ～２００ｍｇ／Ｌであった。 The plant growth regulator of this example is an aqueous solution containing Gokokuho, and the concentration of Gokokuho in the plant growth regulator was 0.5 mg/L to 200 mg/L.
トウモロコシの耐寒性を改善するための、本実施例に記載の植物成長調整剤の応用は、具体的には、室温で０．５ｍｇ／Ｌ～２００ｍｇ／Ｌの五穀豊水溶液を使用して種子をコーティングすることであった。 Specifically, the application of the plant growth regulator described in this example to improve the cold tolerance of corn involves seeding using a Gokokuho water solution of 0.5 mg/L to 200 mg/L at room temperature. It was to coat.
具体的なステップは以下のとおりであった。
（１）五穀豊５０ｍｇを秤量し、脱イオン水で溶解し、１００ｍＬに定容し、５００ｍｇ／Ｌの五穀豊母液を調製した。
（２）ステップ（１）の体積が異なる母液、膜形成剤ポリビニルアルコール５％、分散剤リグニンスルホン酸ナトリウム２％、増粘剤キサンタンガム１％、湿潤剤脂肪酸１％を取り、残りは水で、０．５ｍｇ／Ｌ～２００ｍｇ／Ｌ間の濃度が異なる五穀豊種子コーティング剤を調製し、各濃度を表５に示した。
（３）実がよく入っている均一なトウモロコシ品種徳美亜１号の種子を選択し、体積パーセント濃度５％の次亜塩素酸ナトリウムで３分間消毒し、蒸留水で３回すすぎ、五穀豊種子コーティング剤で種子と混ぜ、薬剤を均一に混合した後に使用に備えるために乾燥させた。
（４）播種する前に、完全な種皮、均一なサイズ、および実がよく入っているトウモロコシの種子を選択し、底部に湿った砂の層で覆われているプラスチックの箱に播種し、約１ｃｍの湿った砂で覆われた各ポットにそれぞれ１０個の種を播き、ライトインキュベーターで培養し、温度を１５℃に設定し、７日後に発芽率を測定した。
The specific steps were as follows.
(1) 50 mg of Gokokuho was weighed, dissolved in deionized water, and the volume was adjusted to 100 mL to prepare 500 mg/L Gokokuho mother liquor.
(2) Take mother liquor of different volumes from step (1), film forming agent polyvinyl alcohol 5%, dispersing agent sodium lignin sulfonate 2%, thickening agent xanthan gum 1%, wetting agent fatty acid 1%, the rest is water, Five grain seed coating agents having different concentrations between 0.5 mg/L and 200 mg/L were prepared, and the respective concentrations are shown in Table 5.
(3) Select seeds of the uniform corn variety Deku Mia No. 1 with good grains, disinfect them with sodium hypochlorite at a volume percent concentration of 5% for 3 minutes, rinse with distilled water 3 times, and prepare Gokoku Seeds. The seeds were mixed with a coating agent, and after the drug was evenly mixed, they were dried for use.
(4) Before sowing, select corn seeds with complete seed coat, uniform size, and well-contained kernels, and sow them into a plastic box covered with a layer of moist sand at the bottom, about Ten seeds were sown in each pot covered with 1 cm of moist sand, cultured in a light incubator, the temperature was set at 15°C, and the germination rate was measured after 7 days.
結果は、濃度が異なる五穀豊はすべてトウモロコシ種子の低温耐性を改善できる。五穀豊濃度がそれぞれ０．５ｍｇ／Ｌ、１０ｍｇ／Ｌ、３０ｍｇ／Ｌ、５０ｍｇ／Ｌ、１００ｍｇ／Ｌ、２００ｍｇ／Ｌである場合、発芽率はそれぞれ２３％、４３％、６１％、７２％、７０％、３７で、対照群の２０％の発芽率よりも顕著に高いことがわかった。 The results show that different concentrations of grain enrichment can all improve the low temperature tolerance of maize seeds. When the grain enrichment concentrations are 0.5 mg/L, 10 mg/L, 30 mg/L, 50 mg/L, 100 mg/L, and 200 mg/L, respectively, the germination rates are 23%, 43%, 61%, 72%, respectively. 70%, 37%, was found to be significantly higher than the 20% germination rate of the control group.
Claims (20)
前記ストレス耐性は、耐寒性、耐塩性、耐アルカリ性及び耐乾性を含み、
前記ストレス耐性が耐寒性、耐塩性、又は耐アルカリ性である場合に、前記植物成長調整剤中の前記五穀豊の濃度は０．５ｍｇ／Ｌ～２００ｍｇ／Ｌであり、
前記ストレス耐性が耐乾性である場合に、前記植物成長調整剤中の前記五穀豊の濃度は３０ｍｇ／Ｌ～２００ｍｇ／Ｌである
前記植物成長調整剤。 A plant growth regulator for stress tolerance in plants , which is an aqueous solution containing Gokokuho as an active ingredient,
The stress resistance includes cold resistance, salt resistance, alkali resistance and drought resistance,
When the stress tolerance is cold tolerance, salt tolerance, or alkali tolerance, the concentration of Gokokuho in the plant growth regulator is 0.5 mg/L to 200 mg/L,
When the stress tolerance is drought tolerance, the concentration of Gokokuho in the plant growth regulator is 30 mg/L to 200 mg/L .
前記ストレス耐性が耐乾性である場合に、前記植物成長調整剤中の前記五穀豊の濃度は３０ｍｇ／Ｌ～１００ｍｇ／Ｌである、請求項１に記載の植物成長調整剤。 When the stress tolerance is cold tolerance, salt tolerance, or alkali tolerance, the concentration of Gokokuho in the plant growth regulator is 30 mg / L to 100 mg / L,
The plant growth regulator according to claim 1, wherein when the stress tolerance is drought tolerance, the concentration of the Gokokuho in the plant growth regulator is 30 mg/L to 100 mg/L.
前記ストレス耐性が耐乾性である場合に、前記植物成長調整剤中の前記五穀豊の濃度は５０ｍｇ／Ｌ～１００ｍｇ／Ｌである、請求項１に記載の植物成長調整剤。 When the stress tolerance is cold tolerance, salt tolerance, or alkali tolerance, the concentration of Gokokuho in the plant growth regulator is 50 mg / L to 100 mg / L,
The plant growth regulator according to claim 1, wherein when the stress tolerance is drought tolerance, the concentration of the Gokokuho in the plant growth regulator is 50 mg/L to 100 mg/L.
前記ストレス耐性が耐乾性である場合に、前記植物成長調整剤中の前記五穀豊の濃度は５０ｍｇ／Ｌである、請求項１に記載の植物成長調整剤。 When the stress tolerance is cold tolerance, salt tolerance, or alkali tolerance, the concentration of Gokokuho in the plant growth regulator is 50 mg/L,
The plant growth regulator according to claim 1, wherein when the stress tolerance is drought tolerance, the concentration of the Gokokuho in the plant growth regulator is 50 mg/L.
前記ストレス耐性は、耐寒性、耐塩性、耐アルカリ性及び耐乾性を含み、 The stress resistance includes cold resistance, salt resistance, alkali resistance and drought resistance,
前記ストレス耐性が耐寒性、耐塩性、又は耐アルカリ性である場合に、前記植物成長調整剤中の前記五穀豊の濃度は０．５ｍｇ／Ｌ～２００ｍｇ／Ｌであり、 When the stress tolerance is cold tolerance, salt tolerance, or alkali tolerance, the concentration of Gokokuho in the plant growth regulator is 0.5 mg/L to 200 mg/L,
前記ストレス耐性が耐乾性である場合に、前記植物成長調整剤中の前記五穀豊の濃度は３０ｍｇ／Ｌ～２００ｍｇ／Ｌである When the stress tolerance is drought tolerance, the concentration of the Gokokuho in the plant growth regulator is 30 mg/L to 200 mg/L.
前記植物成長調整剤の使用方法。A method of using the plant growth regulator.
前記ストレス耐性が耐乾性である場合に、前記植物成長調整剤中の前記五穀豊の濃度は３０ｍｇ／Ｌ～１００ｍｇ／Ｌである、請求項１１に記載の使用方法。 The method of use according to claim 11, wherein when the stress tolerance is drought tolerance, the concentration of the Gokokuho in the plant growth regulator is 30 mg/L to 100 mg/L.
前記ストレス耐性が耐乾性である場合に、前記植物成長調整剤中の前記五穀豊の濃度は５０ｍｇ／Ｌ～１００ｍｇ／Ｌである、請求項１１に記載の使用方法。 The method of use according to claim 11, wherein when the stress tolerance is drought tolerance, the concentration of the Gokokuho in the plant growth regulator is 50 mg/L to 100 mg/L.
前記ストレス耐性が耐乾性である場合に、前記植物成長調整剤中の前記五穀豊の濃度は５０ｍｇ／Ｌである、請求項１１に記載の使用方法。 12. The method of use according to claim 11, wherein when the stress tolerance is drought tolerance, the concentration of Gokokuho in the plant growth regulator is 50 mg/L.
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