CN113854090B - Seedling raising method for improving perennial root performance of sugarcane - Google Patents
Seedling raising method for improving perennial root performance of sugarcane Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/55—Sugar cane
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
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- Agronomy & Crop Science (AREA)
- Soil Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
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- Forests & Forestry (AREA)
- Botany (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to the technical field of agricultural planting, and particularly discloses a seedling raising method for improving perennial root performance of sugarcane, which comprises the following steps: s1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate; s2, soaking the single-bud segments of the sugarcane seed stems with seed soaking liquid before sowing; s3, seedling raising is carried out by adopting a degradable cylindrical paper cup, before seeding, the degradable cylindrical paper cup is paved with a substrate with the thickness of 5-8cm and sterilized in the step S1, after water is thoroughly poured, the substrate is sown into a single-bud section of the seed stem of the sugarcane, and then a layer of the substrate is covered; s4, transplanting the paper cups which are sowed into the sugarcane seed stems and the single buds and filled with the matrix into a greenhouse for seedling stage management. According to the technical scheme, the survival rate of newly planted sugarcane is high, the number of effective seedlings is large, the root system activity of the sugarcane is influenced in the seedling growing process, the adaptability and the stress resistance of the sugarcane root to external environment change are improved, and the quality of the ratoon sugarcane root is improved, so that the rooting rate of the ratoon sugarcane is improved, the ratoon sugarcane root is finally improved, and the economic benefit of sugarcane planting is increased.
Description
Technical Field
The invention belongs to the technical field of agricultural planting, and particularly relates to a seedling raising method for improving perennial root performance of sugarcane.
Background
Sugarcane is an important sugar crop and economic crop in China, guangxi is the largest sugar production base in China, the planting area, the sugarcane yield and the sugar yield of the sugarcane are the first place in China, and the proportion of the sugarcane is over 60%. However, due to the shortage of labor force, the phenomenon that people are difficult to ask for manpower and expensive in the sugar cane area in Guangxi is increasingly serious, and due to the rising of agricultural prices and other reasons, the production cost of the sugar cane is continuously increased, the yield of the planted sugar cane is reduced, the planting area is reduced year by year, the sugar cane industry is facing a severe situation, and how to improve the yield of the planted sugar cane is greatly valued and concerned. The perennial root sugarcane cultivation can save the planting cost of sugarcane seeds, manpower, machine cultivation and the like, and has long growth period, high yield and high income, so the perennial root performance of the sugarcane is very important. At present, the host cultivated varieties in Guangxi regions have weak perennial root performance, and the perennial root smut is serious, so that the effective stems are few, the perennial root sugarcane yield is low, the perennial root age is short, and the perennial root can only be remained for 1-2 years generally. Related research needs to be carried out to improve the perennial root performance of the sugarcane.
Disclosure of Invention
The invention aims to provide a seedling raising method capable of improving the perennial root performance of sugarcane, which can ensure high survival rate and more effective seedlings of newly planted sugarcane, and improve the adaptability and stress resistance of the sugarcane root to external environment changes and the quality of the perennial root of sugarcane by influencing the vitality of the sugarcane root in the seedling raising process, thereby improving the plant growth rate of the perennial root of sugarcane and finally improving the perennial root performance of sugarcane.
In order to realize the aim, the invention provides a seedling raising method for improving the perennial root performance of sugarcane, which comprises the following steps:
s1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate, wherein the seedling substrate consists of 3-4 parts of pond sludge, 1-2 parts of perlite, 2-3 parts of vermiculite, 1-2 parts of peat soil and 2-3 parts of cassava dregs in parts by weight;
s2, cutting the sugarcane seed stems into single-bud segments, and soaking the single-bud segments of the sugarcane seed stems with a seed soaking solution before sowing, wherein the seed soaking solution comprises 30-100 mg/L of uniconazole, 0.1-0.5 mg/L of S-abscisic acid and 5-80 mg/L of eriodictyol;
s3, seedling raising is carried out by adopting a degradable cylindrical paper cup with the diameter of 6-10 cm and the height of 10-15 cm, a substrate with the thickness of 5-8 cm and subjected to disinfection and sterilization in the step S1 is paved before seeding in the paper cup, the substrate is sowed into a single bud section of the sugarcane seed stem after water is poured, and then the paper cup is filled with the substrate;
s4, transplanting the paper cups which are sown into the sugarcane seed stem single bud segments and filled with the matrix into a greenhouse for seedling stage management, wherein the greenhouse is covered by a film, a sunshade net covers the greenhouse, and the seedling stage management comprises the following steps: controlling the temperature and humidity in the greenhouse in the germination process of the seed stems and buds, wherein the temperature is kept at 30 +/-3 ℃, the humidity is kept at 80 +/-3%, the temperature is higher than 33 ℃, films at two ends of the greenhouse are opened for ventilation, and the films are closed when the temperature is lower than 27 ℃; after 3 leaves grow out from the single bud section of the sugarcane seed stem, opening films at two ends of the greenhouse for ventilation, timely removing diseased seedlings, periodically applying water and fertilizer, and spraying insecticide and bactericide for pest control; when the sugarcane seedlings grow to 15-20 cm, the sugarcane seedlings are transplanted to a field together with the substrate.
Further, in the above technical scheme, in the step S1, the seed soaking solution soaks the single-bud segments of the sugarcane seed stems for 3-5 hours, and is stirred once every 1 hour.
Further, in the above technical scheme, in the step S1, the temperature of the seed soaking liquid for soaking the single bud section of the sugarcane seed stem is 45-50 ℃.
Further, in the above technical scheme, in the step S2, the seed stems of the sugarcane are cut at both ends of the knot, and the cut seed stems are cut into single bud segments with a length of 3-4 cm.
Further, in the above technical scheme, the sunshade rate of the sunshade net in the step S4 is 30% to 40%.
Further, in the above technical scheme, the step S3 further includes watering the seedling with a plant juice diluent every two days after 3 leaves are grown from the single bud section of the sugarcane seed stem, wherein the plant juice diluent is obtained by mixing succulent, radix hemerocallis and radix paeoniae rubra root, juicing, diluting with water, filtering, and removing the filter residue.
Further, in the above technical solution, in the step S4, the mass ratio of the succus to the hemerocallis fulva to the paeonia lactiflora is (3-5): (7-11): (11-13).
Further, in the above technical scheme, the vermiculite in the step S1 is modified vermiculite, and the preparation method of the modified vermiculite comprises:
(1) Crushing vermiculite, sieving with a 200-mesh sieve to obtain vermiculite powder with the particle size of less than 200 meshes, soaking the vermiculite powder in a hydrochloric acid solution with the mass concentration of 20-30% for 12-15 hours, filtering, washing with water to be neutral, and drying;
(2) Diluting 10% of Dufulin emulsion by 200 times with deionized water, stirring and mixing uniformly, adding dried vermiculite powder into the diluent, mixing and ultrasonically treating for 30-40 minutes, wherein the mass ratio of the vermiculite powder to the diluent is 1:10 to 15;
(3) Filtering the mixture after ultrasonic treatment, and drying the vermiculite powder to obtain the modified vermiculite powder.
Further, in the above technical solution, the 10% Dufulin emulsifiable concentrate in the step (2) is formed by mixing and dissolving 10% Dufulin, 15% N, N-dimethylformamide, 3% calcium dodecylbenzene sulfonate, 5% alkylphenol ethoxylates, 2% castor oil ethoxylates and xylene to 100% and making it transparent.
Further, in the above technical scheme, the fertilizer for fertilization in the step S4 is a 30-10-10 soluble fertilizer, and a 0.3% soluble fertilizer solution is sprayed on the leaf surface. .
Compared with the prior art, the invention has the following beneficial effects:
1. the method improves the perennial root property of the sugarcane through the processes of seed stem soaking of the sugarcane, plant juice diluent pouring in the seedling raising process of the sugarcane and the like, is beneficial to cultivation of the perennial root sugarcane, saves the cost, improves the yield of the perennial root sugarcane, and improves the economic benefit of sugarcane planting.
2. The sugarcane seedling raising process adopts the seed soaking liquid containing the active components of uniconazole, S-abscisic acid and eriodictyol to soak, which is favorable for shortening the sugarcane seedling raising period and improving the sugarcane seedling raising efficiency.
3. According to the method, the two processes of applying the plant juice diluent to the seedlings in the seedling raising process and sterilizing the sugarcane seedling raising matrix by high-temperature steam are combined, so that the capability of the sugarcane seedlings for resisting external pest and disease attack is enhanced, and the probability of pathogen infection of the sugarcane seedlings is reduced in the germination and growth process of the single-bud segments of the sugarcane seed stems, so that the influence of the pest and disease on the sugarcane seedling raising and subsequent transplanting growth processes is reduced, the sugarcane planting income increase is promoted, and the frequency of pest control by spraying pesticides is reduced.
4. The modified vermiculite is obtained by soaking vermiculite in a hydrochloric acid solution to modify the vermiculite, and adsorbing antiviral and antibacterial active ingredients by the modified vermiculite under the ultrasonic auxiliary condition, and the modified vermiculite is used in a seedling culture substrate, so that the water retention capacity of the substrate is improved, and the modified vermiculite has the effect of improving soil after being transplanted to a field at a later stage, and has the effect of preventing variety degradation caused by repeated infection of sugarcane stumps by pathogens such as sugarcane mosaic disease, perennial root dwarfing and the like after being absorbed by sugarcane seedlings through the root parts due to the adsorption of the antiviral and antibacterial active ingredients. Meanwhile, the toxic fluorine phosphorus adsorbed by the pores of the modified vermiculite is slowly released, so that the release period is long, the drug effect period is long, the reduction of the yield, the reduction of the root quality and the reduction of the plant growth rate are facilitated, and the perennial root property of the sugarcane is improved.
Detailed Description
Specific embodiments of the present invention are described in detail below with reference to the tables, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Example 1
S1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate, wherein the seedling substrate consists of 4 parts of pond sludge, 2 parts of perlite, 3 parts of modified vermiculite, 2 parts of peat soil and 3 parts of cassava dregs in parts by weight;
s2, cutting the sugarcane seed stems at two ends of the knots, cutting the sugarcane seed stems into seed stem single-bud sections with the length of 3-4 cm, soaking the sugarcane seed stem single-bud sections with a seed soaking solution before sowing, soaking the sugarcane seed stem single-bud sections with the seed soaking solution for 5 hours, stirring the single-bud sections once every 1 hour, wherein the temperature of the seed soaking solution for soaking the sugarcane seed stem single-bud sections is 45-50 ℃, and the seed soaking solution contains 100mg/L of uniconazole, 0.5mg/L of S-abscisic acid and 80mg/L of eriodictyol;
s3, seedling raising is carried out by adopting a degradable cylindrical paper cup with the diameter of 6-10 cm and the height of 10-15 cm, a substrate which is sterilized in the step S1 and has the thickness of 8cm is paved before seeding of the degradable cylindrical paper cup, the substrate is sowed into a single bud section of the sugarcane seed stem after water is poured, and then the substrate is filled in the paper cup;
s4, transplanting the paper cup which is sown into the sugarcane seed stem single bud section and filled with the matrix into a greenhouse for seedling stage management, wherein the greenhouse is covered by a film, a sun-shading net is covered on the greenhouse, the sun-shading rate of the sun-shading net is 30% -40%, and the seedling stage management comprises the following steps: controlling the temperature and humidity in the greenhouse during the seed stem germination process, wherein the temperature is kept at 30 +/-3 ℃, the humidity is kept at 80 +/-3%, the temperature is higher than 33 ℃, films at two ends of the greenhouse are opened for ventilation, and the films are closed when the temperature is lower than 27 ℃; after 3 leaves grow out from a single bud section of a sugarcane seed stem, opening thin films at two ends of a greenhouse for ventilation, removing diseased seedlings in time, pouring plant juice diluent for the seedlings every two days, simultaneously applying water and fertilizer, namely regularly spraying a soluble fertilizer aqueous solution with the mass concentration of 0.3% by adopting a leaf surface spraying mode every 3 days, wherein the fertilizer for fertilization is 30-10-10 soluble fertilizer, spraying an insecticidal bactericide for pest control, the bactericide is chlorothalonil, the insecticide is nitenpyram, the plant juice diluent is obtained by mixing succulent, hemerocallis fulva root and peony root, juicing, adding water for dilution, filtering and removing filter residues, and the mass ratio of succulent to hemerocallis to peony is 5; when the sugarcane seedlings grow to 15-20 cm, the sugarcane seedlings are transplanted to a field together with the substrate.
The preparation method of the modified vermiculite comprises the following steps:
(1) Crushing vermiculite, sieving with a 200-mesh sieve to obtain vermiculite powder with the particle size of less than 200 meshes, soaking the vermiculite powder in a hydrochloric acid solution with the mass concentration of 30% for 12 hours, filtering, washing with water to be neutral, and drying;
(2) Diluting 10% of Dufulin emulsion by 200 times with deionized water, stirring and mixing uniformly, adding dried vermiculite powder into the diluent, mixing and ultrasonically treating for 40 minutes, wherein the mass ratio of the vermiculite powder to the diluent is 1:15; wherein the 10% Dufulin emulsifiable solution is prepared by mixing and dissolving 10% Dufulin original drug, 15% N, N-dimethylformamide, 3% calcium dodecylbenzene sulfonate, 5% alkylphenol polyoxyethylene, 2% castor oil polyoxyethylene and xylene which is supplemented to 100% for transparency.
(3) Filtering the mixture after ultrasonic treatment, and drying the vermiculite powder to obtain the modified vermiculite.
Example 2
S1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate, wherein the seedling substrate consists of 4 parts of pond sludge, 2 parts of perlite, 3 parts of vermiculite, 2 parts of peat soil and 3 parts of cassava dregs in parts by weight;
s2, cutting the sugarcane seed stems at two ends of the knots, cutting the sugarcane seed stems into seed stem single-bud sections with the length of 3-4 cm, soaking the sugarcane seed stem single-bud sections with a seed soaking solution before sowing, soaking the sugarcane seed stem single-bud sections with the seed soaking solution for 5 hours, stirring the single-bud sections once every 1 hour, wherein the temperature of the seed soaking solution for soaking the sugarcane seed stem single-bud sections is 45-50 ℃, and the seed soaking solution contains 100mg/L of uniconazole, 0.5mg/L of S-abscisic acid and 80mg/L of eriodictyol;
s3, seedling raising is carried out by adopting a degradable cylindrical paper cup with the diameter of 6-10 cm and the height of 10-15 cm, a substrate which is sterilized in the step S1 and has the thickness of 8cm is paved before seeding of the degradable cylindrical paper cup, the substrate is sowed into a single bud section of the sugarcane seed stem after water is poured, and then the substrate is filled in the paper cup;
s4, transplanting the paper cup which is sown into the sugarcane seed stem single bud section and filled with the matrix into a greenhouse for seedling stage management, wherein the greenhouse is covered by a film, a sun-shading net is covered on the greenhouse, the sun-shading rate of the sun-shading net is 30% -40%, and the seedling stage management comprises the following steps: controlling the temperature and humidity in the greenhouse during the seed stem germination process, wherein the temperature is kept at 30 +/-3 ℃, the humidity is kept at 80 +/-3%, the temperature is higher than 33 ℃, films at two ends of the greenhouse are opened for ventilation, and the films are closed when the temperature is lower than 27 ℃; after 3 leaves grow out from a single bud section of a sugarcane seed stem, opening thin films at two ends of a greenhouse for ventilation, removing diseased seedlings in time, pouring plant juice diluent for the seedlings every two days, simultaneously applying water and fertilizer, namely regularly spraying a soluble fertilizer aqueous solution with the mass concentration of 0.3% by adopting a leaf surface spraying mode every 3 days, wherein the fertilizer for fertilization is 30-10-10 soluble fertilizer, spraying an insecticidal bactericide for pest control, the bactericide is chlorothalonil, the insecticide is nitenpyram, the plant juice diluent is obtained by mixing succulent, hemerocallis fulva root and peony root, juicing, adding water for dilution, filtering and removing filter residues, and the mass ratio of succulent to hemerocallis to peony is 5; when the sugarcane seedlings grow to 15-20 cm, the sugarcane seedlings are transplanted to a field together with the substrate.
Example 3
S1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate, wherein the seedling substrate consists of 4 parts of pond sludge, 2 parts of perlite, 3 parts of vermiculite, 2 parts of peat soil and 3 parts of cassava dregs in parts by weight;
s2, cutting the sugarcane seed stems at two ends of the knots, cutting the sugarcane seed stems into seed stem single-bud sections with the length of 3-4 cm, soaking the sugarcane seed stem single-bud sections with a seed soaking solution before sowing, soaking the sugarcane seed stem single-bud sections with the seed soaking solution for 5 hours, stirring the single-bud sections once every 1 hour, wherein the temperature of the seed soaking solution for soaking the sugarcane seed stem single-bud sections is 45-50 ℃, and the seed soaking solution contains 100mg/L of uniconazole, 0.5mg/L of S-abscisic acid and 80mg/L of eriodictyol;
s3, seedling raising is carried out by adopting a degradable cylindrical paper cup with the diameter of 6-10 cm and the height of 10-15 cm, a substrate which is sterilized in the step S1 and has the thickness of 8cm is paved before seeding of the degradable cylindrical paper cup, the substrate is sowed into a single bud section of the sugarcane seed stem after water is poured, and then the substrate is filled in the paper cup;
s4, transplanting the paper cup which is sown into the sugarcane seed stem single bud section and filled with the matrix into a greenhouse for seedling stage management, wherein the greenhouse is covered by a film, a sun-shading net is covered on the greenhouse, the sun-shading rate of the sun-shading net is 30% -40%, and the seedling stage management comprises the following steps: controlling the temperature and humidity in the greenhouse during the seed stem germination process, wherein the temperature is kept at 30 +/-3 ℃, the humidity is kept at 80 +/-3%, the temperature is higher than 33 ℃, films at two ends of the greenhouse are opened for ventilation, and the films are closed when the temperature is lower than 27 ℃; after 3 leaves grow out from a single bud section of a sugarcane seed stem, opening thin films at two ends of a greenhouse for ventilation, timely removing diseased seedlings, simultaneously applying water and fertilizer, namely regularly spraying a soluble fertilizer aqueous solution with the mass concentration of 0.3% by adopting a leaf surface spraying mode every 3 days, spraying an insecticide and a bactericide for pest control, wherein the bactericide is chlorothalonil, and the insecticide is nitenpyram; when the sugarcane seedlings grow to 15-20 cm, the sugarcane seedlings are transplanted to a field together with the substrate.
Comparative example 1
S1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate, wherein the seedling substrate consists of 4 parts of pond sludge, 2 parts of perlite, 3 parts of vermiculite, 2 parts of peat soil and 3 parts of cassava dregs in parts by weight;
s2, cutting the sugarcane seed stems at two ends of the knots, cutting the sugarcane seed stems into seed stem single-bud sections with the length of 3-4 cm, soaking the sugarcane seed stem single-bud sections with a seed soaking solution before sowing, soaking the sugarcane seed stem single-bud sections with the seed soaking solution for 5 hours, stirring the sugarcane seed stem single-bud sections once every 1 hour, wherein the temperature of the seed soaking solution for soaking the sugarcane seed stem single-bud sections is 45-50 ℃, and the seed soaking solution consists of uniconazole of 100mg/L and S-abscisic acid of 0.5 mg/L;
s3, seedling raising is carried out by adopting a degradable cylindrical paper cup with the diameter of 6-10 cm and the height of 10-15 cm, a substrate which is sterilized in the step S1 and has the thickness of 8cm is paved before seeding of the degradable cylindrical paper cup, the substrate is sowed into a single bud section of the sugarcane seed stem after water is poured, and then the substrate is filled in the paper cup;
s4, transplanting the paper cup which is sown into the sugarcane seed stem single bud section and filled with the matrix into a greenhouse for seedling stage management, wherein the greenhouse is covered by a film, a sun-shading net is covered on the greenhouse, the sun-shading rate of the sun-shading net is 30% -40%, and the seedling stage management comprises the following steps: controlling the temperature and humidity in the greenhouse during the seed stem germination process, wherein the temperature is kept at 30 +/-3 ℃, the humidity is kept at 80 +/-3%, the temperature is higher than 33 ℃, films at two ends of the greenhouse are opened for ventilation, and the films are closed when the temperature is lower than 27 ℃; after 3 leaves grow out from a single bud section of a sugarcane seed stem, opening thin films at two ends of a greenhouse for ventilation, timely removing diseased seedlings, simultaneously applying water and fertilizer, namely regularly spraying a soluble fertilizer aqueous solution with the mass concentration of 0.3% by adopting a leaf surface spraying mode every 3 days, spraying an insecticide and a bactericide for pest control, wherein the bactericide is chlorothalonil, and the insecticide is nitenpyram; when the sugarcane seedlings grow to 15-20 cm, the sugarcane seedlings are transplanted to a field together with the substrate.
The sugarcane seedlings cultured in the examples 1, 2, 3 and 1 are respectively and randomly selected and transplanted to a field, the sugarcane seedlings with the germinated sugarcane stumps after the sugarcane is cut and harvested are taken as a comparison group 2 to be managed together, the planting management modes and conditions are kept consistent, the plant emergence rate and the average weight of a single stem of the stubble sugarcane after the sugarcane is cut and harvested are statistically calculated, and the statistical results are shown in the table 1.
TABLE 1 growth rate and average weight of single stalks of sugarcane
| Cultivated sugarcane seedlings of different types | Percentage of Hair growth (%) | Average weight of single stem (g) |
| Example 1 | 96.3 | 1875.9 |
| Example 2 | 91.1 | 1856.1 |
| Example 3 | 86.8 | 1839.5 |
| Comparative example 1 | 77.1 | 1796.9 |
| Comparative group 2 | 76.4 | 1787.4 |
As can be seen from table 1, the stubble leaving sugarcane top rate of the seedlings obtained by the method of example 1 is 96.3%, the stubble leaving sugarcane top rate of the seedlings obtained by the method of example 2 is 91.3%, the stubble leaving sugarcane top rate of the seedlings obtained by the method of example 3 is 86.8%, the stubble leaving sugarcane top rate of comparative example 1 and comparative example 2 is 77.1% and 76.4%, respectively, the average single-stem weight of the seedlings obtained by the methods of example 1, example 2 and example 3 is 1875.9g, 1856.1g and 1839.5g, respectively, the stubble leaving sugarcane top rate of comparative example 1 and comparative example 2 is 1796.9g and 1787.4g, respectively, and the effect of improving the sugarcane root bearing performance of the seedlings obtained by the methods of example 1, example 2 and example 3 is obvious.
Example 4
S1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate, wherein the seedling substrate consists of 4 parts of pond sludge, 2 parts of perlite, 3 parts of modified vermiculite, 2 parts of peat soil and 3 parts of cassava dregs in parts by weight;
s2, cutting the sugarcane seed stems at two ends of the knots, cutting the sugarcane seed stems into seed stem single-bud sections with the length of 3-4 cm, soaking the sugarcane seed stem single-bud sections with a seed soaking solution before sowing, soaking the sugarcane seed stem single-bud sections with the seed soaking solution for 5 hours, stirring the single-bud sections once every 1 hour, wherein the temperature of the seed soaking solution for soaking the sugarcane seed stem single-bud sections is 45-50 ℃, and the seed soaking solution contains 100mg/L of uniconazole, 0.5mg/L of S-abscisic acid and 80mg/L of eriodictyol;
s3, seedling raising is carried out by adopting a degradable cylindrical paper cup with the diameter of 6-10 cm and the height of 10-15 cm, a substrate which is sterilized in the step S1 and has the thickness of 8cm is paved before seeding of the degradable cylindrical paper cup, the substrate is sowed into a single bud section of the sugarcane seed stem after water is poured, and then the substrate is filled in the paper cup;
s4, transplanting the paper cup which is sown into the sugarcane seed stem single bud section and filled with the matrix into a greenhouse for seedling stage management, wherein the greenhouse is covered by a film, a sun-shading net is covered on the greenhouse, the sun-shading rate of the sun-shading net is 30% -40%, and the seedling stage management comprises the following steps: controlling the temperature and humidity in the greenhouse during the seed stem germination process, wherein the temperature is kept at 30 +/-3 ℃, the humidity is kept at 80 +/-3%, the temperature is higher than 33 ℃, films at two ends of the greenhouse are opened for ventilation, and the films are closed when the temperature is lower than 27 ℃; after 3 leaves grow out from a single bud section of a sugarcane seed stem, opening thin films at two ends of a greenhouse for ventilation, removing diseased seedlings in time, pouring plant juice diluent for the seedlings every two days, simultaneously applying water and fertilizer, namely regularly spraying a soluble fertilizer aqueous solution with the mass concentration of 0.3% by adopting a leaf surface spraying mode every 3 days, wherein the fertilizer for fertilization is 30-10-10 soluble fertilizer, spraying an insecticidal bactericide for pest control, the bactericide is chlorothalonil, the insecticide is nitenpyram, the plant juice diluent is obtained by mixing succulent, hemerocallis fulva root and peony root, juicing, adding water for dilution, filtering and removing filter residues, and the mass ratio of succulent to hemerocallis to peony is 5; when the sugarcane seedlings grow to 15-20 cm, the sugarcane seedlings are transplanted to a field together with the substrate.
The preparation method of the modified vermiculite comprises the following steps:
(1) Crushing vermiculite, sieving with a 200-mesh sieve to obtain vermiculite powder with the particle size of less than 200 meshes, soaking the vermiculite powder in a hydrochloric acid solution with the mass concentration of 20% for 15 hours, filtering, washing with water to be neutral, and drying;
(2) Diluting 10% of Dufulin emulsion by 200 times with deionized water, stirring and mixing uniformly, adding dried vermiculite powder into the diluent, mixing and ultrasonically treating for 30 minutes, wherein the mass ratio of the vermiculite powder to the diluent is 1:10; wherein the 10% Dufulin emulsifiable solution is prepared by mixing and dissolving 10% Dufulin original drug, 15% N, N-dimethylformamide, 3% calcium dodecylbenzene sulfonate, 5% alkylphenol polyoxyethylene, 2% castor oil polyoxyethylene and xylene which is supplemented to 100% for transparency.
(3) Filtering the mixture after ultrasonic treatment, and drying the vermiculite powder to obtain the modified vermiculite.
Example 5
S1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate, wherein the seedling substrate consists of 3 parts of pond sludge, 1 part of perlite, 2 parts of vermiculite, 1 part of peat soil and 2 parts of cassava dregs in parts by weight;
s2, cutting the sugarcane seed stems at two ends of the knots, cutting the sugarcane seed stems into seed stem single-bud sections with the length of 3-4 cm, soaking the sugarcane seed stem single-bud sections with a seed soaking solution before sowing, soaking the sugarcane seed stem single-bud sections with the seed soaking solution for 3 hours, stirring the seed stem single-bud sections once every 1 hour, wherein the temperature of the seed soaking solution for soaking the sugarcane seed stem single-bud sections is 45-50 ℃, and the seed soaking solution contains 30mg/L of uniconazole, 0.1mg/L of S-abscisic acid and 50mg/L of eriodictyol;
s3, seedling raising is carried out by adopting a degradable cylindrical paper cup with the diameter of 6-10 cm and the height of 10-15 cm, a substrate with the thickness of 5-8 cm and subjected to disinfection and sterilization in the step S1 is paved before seeding of the degradable cylindrical paper cup, the substrate is sowed into a single-bud section of the sugarcane seed stem after water is poured, and then the substrate is filled in the paper cup;
s4, transplanting the paper cup which is sown into the sugarcane seed stem single bud section and filled with the matrix into a greenhouse for seedling stage management, wherein the greenhouse is covered by a film, a sun-shading net is covered on the greenhouse, the sun-shading rate of the sun-shading net is 30% -40%, and the seedling stage management comprises the following steps: controlling the temperature and humidity in the greenhouse during the seed stem germination process, wherein the temperature is kept at 30 +/-3 ℃, the humidity is kept at 80 +/-3%, the temperature is higher than 33 ℃, films at two ends of the greenhouse are opened for ventilation, and the films are closed when the temperature is lower than 27 ℃; after 3 leaves grow out from a single bud section of a sugarcane seed stem, opening thin films at two ends of a greenhouse for ventilation, removing diseased seedlings in time, pouring plant juice diluent for the seedlings every two days, simultaneously applying water and fertilizer, namely regularly spraying a soluble fertilizer aqueous solution with the mass concentration of 0.3% by adopting a leaf surface spraying mode every 3 days, wherein the fertilizer for fertilization is 30-10-10 soluble fertilizer, spraying an insecticidal bactericide for pest control, the bactericide is chlorothalonil, the insecticide is nitenpyram, the plant juice diluent is obtained by mixing succulent, hemerocallis fulva root and peony root, juicing, adding water for dilution, filtering and removing filter residues, and the mass ratio of succulent to hemerocallis to peony is 3; when the sugarcane seedlings grow to 15-20 cm, the sugarcane seedlings are transplanted to a field together with the substrate.
Example 6
S1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate, wherein the seedling substrate consists of 3 parts of pond sludge, 1 part of perlite, 2 parts of vermiculite, 1 part of peat soil and 2 parts of cassava dregs in parts by weight;
s2, cutting the sugarcane seed stems at two ends of the knots, cutting the sugarcane seed stems into seed stem single-bud sections with the length of 3-4 cm, soaking the sugarcane seed stem single-bud sections with a seed soaking solution before sowing, soaking the sugarcane seed stem single-bud sections with the seed soaking solution for 3 hours, stirring the seed stem single-bud sections once every 1 hour, wherein the temperature of the seed soaking solution for soaking the sugarcane seed stem single-bud sections is 45-50 ℃, and the seed soaking solution contains 30mg/L of uniconazole, 0.1mg/L of S-abscisic acid and 50mg/L of eriodictyol;
s3, seedling raising is carried out by adopting a degradable cylindrical paper cup with the diameter of 6-10 cm and the height of 10-15 cm, a substrate with the thickness of 5-8 cm and subjected to disinfection and sterilization in the step S1 is paved before seeding of the degradable cylindrical paper cup, the substrate is sowed into a single-bud section of the sugarcane seed stem after water is poured, and then the substrate is filled in the paper cup;
s4, transplanting the paper cup which is sown into the sugarcane seed stem single bud section and filled with the matrix into a greenhouse for seedling stage management, wherein the greenhouse is covered by a film, a sun-shading net is covered on the greenhouse, the sun-shading rate of the sun-shading net is 30% -40%, and the seedling stage management comprises the following steps: controlling the temperature and humidity in the greenhouse during the seed stem germination process, wherein the temperature is kept at 30 +/-3 ℃, the humidity is kept at 80 +/-3%, the temperature is higher than 33 ℃, films at two ends of the greenhouse are opened for ventilation, and the films are closed when the temperature is lower than 27 ℃; after 3 leaves grow out from a single bud section of a sugarcane seed stem, opening thin films at two ends of a greenhouse for ventilation, timely removing diseased seedlings, simultaneously applying water and fertilizer, namely regularly spraying a soluble fertilizer aqueous solution with the mass concentration of 0.3% by adopting a leaf surface spraying mode every 3 days, spraying an insecticide and a bactericide for pest control, wherein the bactericide is chlorothalonil, and the insecticide is nitenpyram; when the sugarcane seedlings grow to 15-20 cm, the sugarcane seedlings are transplanted to a field together with the substrate.
Comparative example 3
S1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate, wherein the seedling substrate consists of 3 parts of pond sludge, 1 part of perlite, 2 parts of vermiculite, 1 part of peat soil and 2 parts of cassava dregs in parts by weight;
s2, cutting the sugarcane seed stems at two ends of the knots, cutting the sugarcane seed stems into seed stem single-bud sections with the length of 3-4 cm, soaking the sugarcane seed stem single-bud sections with a seed soaking solution before sowing, soaking the sugarcane seed stem single-bud sections with the seed soaking solution for 3 hours, stirring the seed stem single-bud sections once every 1 hour, wherein the temperature of the seed soaking solution for soaking the sugarcane seed stem single-bud sections is 45-50 ℃, and the seed soaking solution contains 30mg/L of uniconazole and 0.1mg/L of S-abscisic acid;
s3, seedling raising is carried out by adopting a degradable cylindrical paper cup with the diameter of 6-10 cm and the height of 10-15 cm, a substrate with the thickness of 5-8 cm and subjected to disinfection and sterilization in the step S1 is paved before seeding of the degradable cylindrical paper cup, the substrate is sowed into a single-bud section of the sugarcane seed stem after water is poured, and then the substrate is filled in the paper cup;
s4, transplanting the paper cup which is sown into the sugarcane seed stem single bud section and filled with the matrix into a greenhouse for seedling stage management, wherein the greenhouse is covered by a film, a sun-shading net is covered on the greenhouse, the sun-shading rate of the sun-shading net is 30% -40%, and the seedling stage management comprises the following steps: controlling the temperature and humidity in the greenhouse during the seed stem germination process, wherein the temperature is kept at 30 +/-3 ℃, the humidity is kept at 80 +/-3%, the temperature is higher than 33 ℃, films at two ends of the greenhouse are opened for ventilation, and the films are closed when the temperature is lower than 27 ℃; after 3 leaves grow out from a single bud section of a sugarcane seed stem, opening thin films at two ends of a greenhouse for ventilation, timely removing diseased seedlings, simultaneously applying water and fertilizer, namely regularly spraying a soluble fertilizer aqueous solution with the mass concentration of 0.3% by adopting a leaf surface spraying mode every 3 days, spraying an insecticide and a bactericide for pest control, wherein the bactericide is chlorothalonil, and the insecticide is nitenpyram; when the sugarcane seedlings grow to 15-20 cm, the sugarcane seedlings are transplanted to a field together with the substrate.
The sugarcane seedlings cultured in the examples 4, 5, 6 and 3 are respectively randomly selected and transplanted to a field, the sugarcane seedlings with the germinated sugarcane stumps after the sugarcane is cut and harvested are taken as a comparison group 4 to be managed together, the planting management modes and conditions are kept consistent, the plant growing rate and the average weight of a single stem of the stubble sugarcane after the sugarcane is cut and harvested are statistically calculated, and the statistical results are shown in a table 2.
TABLE 2 growth rate and average weight of single stalks of stubble sugarcane
| Cultivated sugarcane seedlings of different types | Percentage of Hair growth (%) | Average weight of single stem (g) |
| Example 4 | 94.8 | 1793.6 |
| Example 5 | 90.5 | 1778.8 |
| Example 6 | 85.1 | 1767.7 |
| Comparative example 3 | 75.2 | 1743.6 |
| Comparative group 4 | 74.7 | 1735.9 |
As can be seen from table 2, the stubble leaving sugarcane top rate of the seedlings obtained by the method of example 4 is 94.8%, the stubble leaving sugarcane top rate of the seedlings obtained by the method of example 5 is 90.5%, the stubble leaving sugarcane top rate of the seedlings obtained by the method of example 6 is 85.1%, the stubble leaving sugarcane top rate of comparative example 3 and comparative example 4 is 75.2% and 74.7%, the average weight of the single stalk of the seedlings obtained by the methods of example 4, example 5 and example 6 is 1793.6g, 1778.8g and 1767.7g, the average weight of the single stalk of the seedlings obtained by the methods of comparative example 3 and comparative example 4 is 1743.6g and 1735.9g, and the effect of the seedlings obtained by the methods of example 4, example 5 and example 6 on improving the sugarcane root bearing performance is obvious.
In conclusion, the method ensures high survival rate and more effective seedlings of the newly planted sugarcane through the seedling raising process, improves the adaptability and the stress resistance of the sugarcane root to the change of the external environment through influencing the root system activity of the sugarcane in the seedling raising process, and improves the quality of the perennial sugarcane root, thereby improving the perennial sugarcane plant rate and the single-diameter average weight, finally improving the perennial sugarcane root performance and increasing the economic benefit of sugarcane planting.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (8)
1. A seedling raising method for improving perennial root performance of sugarcane is characterized by comprising the following steps:
s1, carrying out high-temperature steam sterilization on a sugarcane seedling substrate, wherein the seedling substrate consists of 3-4 parts of pond sludge, 1-2 parts of perlite, 2-3 parts of modified vermiculite, 1-2 parts of peat soil and 2-3 parts of cassava residue in parts by mass;
the preparation method of the modified vermiculite comprises the following steps:
(1) Crushing vermiculite, sieving the crushed vermiculite with a 200-mesh sieve to obtain vermiculite powder with the particle size of less than 200 meshes, soaking the vermiculite powder in a hydrochloric acid solution with the mass concentration of 20-30% for 12-15 hours, filtering, washing with water to be neutral, and drying;
(2) Diluting 10% of Dufulin emulsion by 200 times with deionized water, stirring and mixing uniformly, adding dried vermiculite powder into a diluent, and carrying out ultrasonic mixing for 30-40 minutes, wherein the mass ratio of the vermiculite powder to the diluent is 1:10 to 15;
(3) Filtering the mixture after ultrasonic treatment, and drying vermiculite powder to obtain the modified vermiculite;
s2, cutting the sugarcane seed stems into single-bud segments, and soaking the single-bud segments of the sugarcane seed stems with a seed soaking solution before sowing, wherein the seed soaking solution comprises 30-100 mg/L of uniconazole, 0.1-0.5 mg/L of S-abscisic acid and 5-80 mg/L of eriodictyol;
s3, seedling raising is carried out by adopting a degradable cylindrical paper cup with the diameter of 6-10cm and the height of 10-15cm, before seeding in the paper cup, a substrate with the thickness of 5-8cm and sterilized in the step S1 is paved, the substrate is sowed into a single-bud section of the sugarcane seed stem after water is poured, and then the paper cup is filled with the substrate; after 3 leaves grow out from a single bud section of a sugarcane seed stem, watering seedlings once every two days with plant juice diluent, wherein the plant juice diluent is obtained by mixing succulent, hemerocallis fulva root and paeonia lactiflora root, juicing, adding water for diluting, filtering and removing filter residues;
s4, transplanting the paper cups which are sown into the sugarcane seed stem single bud segments and filled with the matrix into a greenhouse for seedling stage management, wherein the greenhouse is covered by a film, a sunshade net covers the greenhouse, and the seedling stage management comprises the following steps: controlling the temperature and humidity in the greenhouse in the germination process of the seed stems and buds, wherein the temperature is kept at 30 +/-3 ℃, the humidity is kept at 80 +/-3%, the temperature is higher than 33 ℃, films at two ends of the greenhouse are opened for ventilation, and the films are closed when the temperature is lower than 27 ℃; after 3 leaves grow out from the single bud section of the sugarcane seed stem, opening films at two ends of the greenhouse for ventilation, timely removing diseased seedlings, periodically applying water and fertilizer, and spraying insecticide and bactericide for pest control; when the sugarcane seedlings grow to 15-20cm, transplanting the sugarcane seedlings to a field with the medium.
2. A seedling raising method for improving the perennial root performance of the sugarcane according to claim 1, wherein in the step S1, the seed soaking solution soaks the single stem bud of the sugarcane for 3 to 5 hours and is stirred once every 1 hour.
3. A seedling raising method for improving the perennial root performance of the sugarcane according to claim 1, wherein in the step S1, the temperature of the seed soaking liquid for soaking the single bud of the sugarcane seed stem is 45-50 ℃.
4. A seedling raising method for improving the perennial root quality of sugarcane according to claim 1, wherein in the step S2, the seed stems of sugarcane are cut at two ends of the knot, and the cut seed stems are cut into single bud segments with the length of 3-4 cm.
5. A seedling raising method for improving sugarcane ratoon property according to claim 1, wherein the sun-shading rate of the sun-shading net in the step S4 is 30% -40%.
6. A seedling raising method for improving the perennial root performance of sugarcane according to claim 1, wherein in the step S3, the mass ratio of succulent to hemerocallis fulva to peony is (3 to 5): (7 to 11): (11 to 13).
7. A seedling raising method for improving the perennial root quality of sugarcane according to claim 1, wherein the 10% duffon emulsifiable concentrate in step (2) is formed by mixing, dissolving and clearing 10% duffon original drug, 15% by weight N, N-dimethylformamide, 3% calcium dodecylbenzenesulfonate, 5% alkylphenol ethoxylate, 2% castor oil ethoxylate and xylene to 100%.
8. A seedling raising method for improving sugarcane ratoon property according to claim 1, wherein the fertilizer for fertilization in the step S4 is 30-10-10 soluble fertilizer, and a foliar spray soluble fertilizer solution with the mass concentration of 0.3% is adopted.
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Application publication date: 20211231 Assignee: Nanning Cane Ding Agriculture Co.,Ltd. Assignor: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046314 Denomination of invention: A Seedling Raising Method for Improving Sugarcane Rooting Ability Granted publication date: 20230418 License type: Common License Record date: 20231108 |