JP2022523241A - Plant growth regulators and their applications - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a plant growth regulator and its application. SOLUTION: The plant growth regulator and its application belong to the field of agricultural science and technology. To improve the stress tolerance of crops, the present invention provides plant growth regulators. The plant growth regulator is an aqueous solution containing five-grain abundance, and the concentration of the five-grain abundance as an active ingredient in the plant growth regulator is 0.5 mg / L to 200 mg / L. Different application methods and concentrations are used, depending on the different growth stages of the plant, and the plant growth regulator can be used on the seeds, whole, part, or cultivation medium of the crop to make the crop stress resistant. It can be significantly enhanced and promote the healthy growth of plants. [Selection diagram] None

Description

The present invention belongs to the field of agricultural science and technology, and particularly relates to plant growth regulators and their applications.

During the production of crops and vegetables, we often encounter abiological stresses such as drought, salt and extreme temperature, which have a serious impact on the normal growth and development of the plant and ultimately of the plant. Causes death and reduced yield. The use of beneficial extrinsic plant growth regulators, based on improvements in plant varieties, techniques and planting patterns, has important economic significance for improving plant stress tolerance. Wugufengin, also known as decoyinine and angustmycin A, is soluble in water, highly thermostable, and has little cytotoxicity. Injection of 2.5 g / kg into the abdominal cavity of mice has no toxic adverse reactions.

The present invention provides a plant growth regulator to improve the stress tolerance of crops.

The plant growth regulator is an aqueous solution containing five-grain growth regulator, and the concentration of the five-grain growth regulator as an active ingredient is 0.5 mg / L to 200 mg / L.

Preferably, the concentration of the five grains in the plant growth regulator is 30 mg / L to 100 mg / L.

More preferably, the concentration of the five grains in the plant growth regulator is 50 mg / L.

The present invention also provides the application of the plant growth regulator in improving the stress tolerance of plants.

More specifically, the plant seeds, whole plant, tissue portion or culture medium of the plant is treated with the above plant growth regulator.

More specifically, the treatment is a spraying treatment on a plant or plant seed, or a spraying treatment or irrigation treatment on the soil surrounding the plant.

More specifically, the plant spraying process refers to spraying the foliage of the plant, and the plant seed spraying process refers to dipping or coating the plant seeds with a plant growth regulator.

More specifically, the timing of the spray is before or during adversity stress of the plant.

More specifically, the application refers to the application of a plant growth preparation for improving stress tolerance of wheat, rice, corn or soybean.

Further limiting, the stress resistance includes cold resistance, salt resistance, alkali resistance, and drought resistance.

The present invention utilizes a method of improving the stress tolerance of crops using five-grain abundance to reduce damage caused by drought, alkalinity, and extreme temperature of plants, promote plant growth and development, crops and The purpose is to study increasing the yield of vegetables.

The application concentration of the plant growth regulator containing the five-grain abundance of the present invention is 0.5 mg / L to 200 mg / L, preferably 30 mg / L to 100 mg / L. Depending on the different growth stages of the plant, different application methods and concentrations can be used in the seeds of the plant (or crop), the whole plant, parts of the plant, or the culture medium of the plant. The method of the present invention is easy to carry out and operate, and as an active ingredient, five-grain abundance can significantly increase the resistance of crops in a short period of time, and the duration of the effect is long, thereby planting. Promotes healthy growth.

FIG. 1 shows the structure of five-grain abundance.

Hereinafter, the present invention will be described in more detail with reference to examples, but the content of the present invention is not limited.

The five-grain abundance of the present invention can be prepared by microbial synthesis. Streptomyces genus NEAU6 is fermented and cultured, and the culture solution is extracted to prepare a compound five-grain abundance. The Streptomyces sp. NEAU6 was deposited at the China Microbial Species Conservation Management Committee Ordinary Microbial Center on August 24, 2018, and the deposit address is No. 3, Hokushin West Road, Chaoyang District, Beijing, Beijing. The deposit number is CGMCC 16338.

The media involved are:
Takashi No. 1 agar medium: soluble starch 20 g, NaCl 0.5 g, KNO ₃₁ g, FeSO ₄ . 7H ₂ O 0.01g, K ₂ HPO ₄ 0.5g, Л ₄ . 7H ₂ O 0.5 g, agar 20 g, distilled water 1000 ml, pH 7.2-7.4.
Seed medium: tryptone 17 g, soybean peptone 3 g, NaCl 5 g, K ₂ HPO ₄ 2.5 g, glucose 2.5 g, distilled water 1000 ml, pH 7.2-7.4.
Fermentation medium: soluble starch 10 g, glucose 10 g, glycerin 10 g, tryptone 5 g, yeast extract 5 g, CaCO _{3 3} g, tap water 1000 ml, pH 7.2-7.4.

Under aseptic conditions, the spores of the NEAU6 strain activated on the Takashi No. 1 agar medium were scraped off, transferred to the seed medium, cultured at 30 ° C. and 200 rpm for 2 days, and then the seed solution was 6% (v / v). ) Inoculate the fermentation medium and incubate at 30 ° C. and 200 rpm for 5 days. The cells are removed by centrifugation, the supernatant is extracted 3 to 4 times with the same volume of ethyl acetate, the extract is concentrated, and then silica gel column chromatography, gel chromatography, and C-18 column chromatography are performed in this order. The compound is obtained after chromatography. The structure of the compound was determined by 1H-NMR, 13C-NMR, and MS spectral analysis, and the structural formula is shown in FIG.

Related plant varieties, film-forming agent polyvinyl alcohol, dispersant sodium lignosulfonate, thickener xanthan gum, wetting agent fatty acids, and laboratory equipment can all be purchased through a commercial approach.

Example 1. Application of plant growth regulators in improving the drought resistance of rice.

The plant growth regulator of this example was an aqueous solution containing five-grain abundance, and the concentration of the five-grain abundance in the plant growth regulator was 0.5 mg / L to 200 mg / L.

The application of the plant growth regulator described in this example to improve the drought resistance of rice refers to immersing rice seeds in a five-grain aqueous solution having a concentration of 0.5 mg / L to 200 mg / L at room temperature. The soaking time may be in the range of 12 to 24 hours.

The specific steps were as follows.
(1) 50 mg of five-grain abundance was weighed, dissolved in deionized water, and the volume was adjusted to 100 mL to prepare a 500 mg / L five-grain abundant mother liquor.
(2) Take mother liquor with different volumes in step (1), add deionized water according to the ratio, dilute with five-grain rich aqueous solution with different concentrations between 0.5 mg / L and 200 mg / L, and show each concentration. Shown in 1.
(3) Select uniform rice dragon cake 31 seeds containing a lot of fruits, disinfect with sodium hypochlorite having a volume fraction concentration of 5% for 3 minutes, rinse with distilled water 3 times, and then the concentration gradient is different. The seeds were soaked in a five-grain aqueous solution for 48 hours.
(4) Germination experiment of rice cultivar Ryukyu 31 seeds under drought stress was carried out: The soaked seeds were transferred to a Petri dish with a diameter of 9 cm, 16 rice seeds were placed in each Petri dish, and repeated 3 times. .. Next, add a 10% mass percentage PEG-6000 solution to each Petri dish to simulate drought stress, replace the PEG-6000 solution every 12 hours, and observe the germination of rice seeds after 4 days. , Statistically measured the root length and germination length of rice. The results are shown in Table 1.

From Table 1, it was found that all the five-grain aqueous solutions having different concentrations can improve the drought resistance of rice seeds. From the comparison of germination rate, root length, and bud length, soaking seeds with five-grain abundance reduces the drought stress of rice seeds, greatly improves germination and growth of rice seeds, and 50 mg / L. It was found that the shoot length and root length were increased by 34.9% and 65.4%, respectively, as compared with the control.

Example 2. Application of plant growth regulators in improving salt stress in rice.

The plant growth regulator of this example was an aqueous solution containing five-grain abundance, and the concentration of the five-grain abundance in the plant growth regulator was 0.5 mg / L to 200 mg / L.

The application of the plant growth regulator described in this example for improving the salt stress capacity of rice is to perform root dipping treatment using a 0.5 mg / L to 200 mg / L five-grain aqueous solution at room temperature. Pointed to.

The specific steps were as follows.
(1) 50 mg of five-grain abundance was weighed, dissolved in deionized water, and the volume was adjusted to 100 mL to prepare a 500 mg / L five-grain abundant mother liquor.
(2) Take mother liquor with different volumes in step (1), add deionized water according to the ratio, dilute with five-grain rich aqueous solution with different concentrations between 0.5 mg / L and 200 mg / L, and show each concentration. Shown in 2.
(3) Select uniform rice cultivar Ryukyu 31 seeds containing a lot of fruits, disinfect with sodium hypochlorite having a volume fraction concentration of 5% for 3 minutes, rinse with distilled water 3 times, and turn the seeds into distilled water. Soaked.
(4) Uniform seeds were picked up, placed in a Petri dish with a diameter of 9 cm, cultured in distilled water, and when the seedlings grew into one leaf and one core, they were cultured in two leaves and one core using a nutrient solution. Next, the roots were soaked for 24 hours using an aqueous solution of five grains with different concentration gradients, then a 0.2 mol / L sodium chloride solution was added for stress treatment, and the sodium chloride solution was applied every 24 hours. Was replaced, and after 3 days, the height of the rice and the fresh weight of the roots were measured. The results are shown in Table 2.

From Table 2, it is possible to improve the salt tolerance of rice by immersing the roots of rice in five-grain abundance with different concentrations depending on the seedling stage and then performing salt stress. When the five-grain abundance concentration was 50 mg / L, the effect was highest, and the height, the fresh weight including the seedlings, and the fresh weight of the roots were 42.5%, 31.6%, and, respectively, as compared with the control. It was found that there was an increase of 19.6%.

Example 3. Application of plant growth regulators in improving salt tolerance of rice.

The plant growth regulator of this example was an aqueous solution containing five-grain abundance, and the concentration of the five-grain abundance in the plant growth regulator was 0.5 mg / L to 200 mg / L.

The application of the plant growth regulator described in this example for improving the salt tolerance of rice is specifically sprayed on the leaf surface using a 0.5 mg / L to 200 mg / L five-grain aqueous solution at room temperature. The spray amount was 0.1 L / m ² , sprayed 2-3 times.

The specific steps were as follows.

(1) 50 mg of five-grain abundance was weighed, dissolved in deionized water, and the volume was adjusted to 100 mL to prepare a 500 mg / L five-grain abundant mother liquor.
(2) Take mother liquor with different volumes in step (1), add deionized water according to the ratio, dilute with five-grain rich aqueous solution with different concentrations between 0.5 mg / L and 200 mg / L, and show each concentration. Shown in 3.
(3) Select uniform rice cultivar Ryukyu 31 seeds containing a lot of fruits, disinfect with sodium hypochlorite having a volume fraction concentration of 5% for 3 minutes, rinse with distilled water 3 times, and turn the seeds into distilled water. Soaked.
(4) Uniform seeds were picked up, placed in a Petri dish with a diameter of 9 cm, cultured in distilled water, and when the seedlings grew into one leaf and one core, they were cultured in two leaves and one core using a nutrient solution. Next, using a five-grain aqueous solution with different concentration gradients, spray, spray once every 12 hours, spray a total of three times, then add 0.2 mol / L sodium chloride solution for stress. The treatment was performed, the sodium chloride solution was changed every 24 hours, and after 3 days, the height and fresh weight of the rice were measured. The results are shown in Table 3.

From Table 3, the salt tolerance of rice can be enhanced by spraying rice with a five-grain aqueous solution having different concentrations at the seedling stage and then applying salt stress. When the five-grain abundance concentration was 50 mg / L, the effect was the highest, and the height, the fresh weight including the seedlings, and the fresh weight of the roots were 46.1%, 27.7% and 19 respectively as compared with the control. It turned out that it increased by%.

Example 4. Application of plant growth regulators in improving the alkali resistance of rice.

The plant growth regulator of this example was an aqueous solution containing five-grain abundance, and the concentration of the five-grain abundance in the plant growth regulator was 0.5 mg / L to 200 mg / L.

The application of the plant growth regulator described in this example for improving the alkali resistance of rice is specifically sprayed on the leaf surface using a 0.5 mg / L to 200 mg / L five-grain aqueous solution at room temperature. The spray amount was 0.1 L / m ² , and the spray was performed 2-3 times.

The specific steps were as follows.
(1) 50 mg of five-grain abundance was weighed, dissolved in deionized water, and the volume was adjusted to 100 mL to prepare a 500 mg / L five-grain abundant mother liquor.
(2) Take mother liquor with different volumes in step (1), add deionized water according to the ratio, dilute with five-grain rich aqueous solution with different concentrations between 0.5 mg / L and 200 mg / L, and show each concentration. Shown in 4.
(3) Select uniform rice cultivar Ryukyu 31 seeds containing a lot of fruits, disinfect with sodium hypochlorite having a volume fraction concentration of 5% for 3 minutes, rinse with distilled water 3 times, and turn the seeds into distilled water. Soaked.
(4) Uniform seeds were picked up, placed in a Petri dish with a diameter of 9 cm, cultured in distilled water, and when the seedlings grew into one leaf and one core, they were cultured in two leaves and one core using a nutrient solution. Next, using a five-grain aqueous solution with different concentration gradients, spray, spray once every 12 hours, spray a total of three times, then add 0.2 mol / L sodium carbonate solution for stress. The treatment was performed, the sodium carbonate solution was changed every 24 hours, and the height and fresh weight of the rice were measured after 3 days. The results are shown in Table 4.

From Table 4, it is possible to increase the alkali resistance of rice by spraying rice with an aqueous solution of five grains having different concentrations depending on the seedling stage and then applying alkaline stress. When the concentration of five-grain abundance was 50 mg / L, the effect was the highest, and the height, the fresh weight including the seedlings, and the fresh weight of the roots were 13.1%, 33% and 30. It was found to have increased by 1%.

Example 5. Application of plant growth regulators in improving cold hardiness of maize.

The plant growth regulator of this example was an aqueous solution containing five-grain abundance, and the concentration of the five-grain abundance in the plant growth regulator was 0.5 mg / L to 200 mg / L.

The application of the plant growth regulator described in this example to improve the cold hardiness of corn is specifically to use 0.5 mg / L to 200 mg / L of a five-grain aqueous solution at room temperature to produce seeds. It was to coat.

The specific steps were as follows.
(1) 50 mg of five-grain abundance was weighed, dissolved in deionized water, and the volume was adjusted to 100 mL to prepare a 500 mg / L five-grain abundant mother liquor.
(2) Take the mother liquor having different volumes in step (1), the film-forming agent polyvinyl alcohol 5%, the dispersant sodium lignin sulfonate 2%, the thickener xanthan gum 1%, and the wetting agent fatty acid 1%, and the rest is water. Five-grain fatty acid coating agents having different concentrations between 0.5 mg / L and 200 mg / L were prepared, and each concentration is shown in Table 5.
(3) Select seeds of Tokumia No. 1, a uniform corn variety that contains a lot of fruits, disinfect with sodium hypochlorite having a volume fraction concentration of 5% for 3 minutes, rinse with distilled water three times, and use five-grain abundant seeds. The seeds were mixed with a coating agent, the agent was evenly mixed and then dried for use.
(4) Before sowing, select corn seeds with full seed coat, uniform size, and good fruit, sow in a plastic box covered with a layer of moist sand at the bottom, about Ten seeds were sown in each pot covered with 1 cm of moist sand, cultured in a light incubator, set to a temperature of 15 ° C., and germination rate was measured after 7 days.

The result is that all five grains with different concentrations can improve the low temperature tolerance of corn seeds. When the five-grain rich concentrations are 0.5 mg / L, 10 mg / L, 30 mg / L, 50 mg / L, 100 mg / L, and 200 mg / L, respectively, the germination rates are 23%, 43%, 61%, and 72%, respectively. At 70% and 37, it was found that the germination rate was significantly higher than the germination rate of 20% in the control group.

Claims (10)

It is an aqueous solution containing five-grain abundance, and the five-grain abundance as an active ingredient is a plant growth regulator having a concentration of 0.5 mg / L to 200 mg / L with respect to the plant growth regulator. The plant growth regulator according to claim 1, wherein the concentration of the five grains in the plant growth regulator is 30 mg / L to 100 mg / L. The plant growth regulator according to claim 1, wherein the concentration of the five grains in the plant growth regulator is 50 mg / L. Application of the plant growth regulator according to any one of claims 1 to 3 in improving stress tolerance of plants. The application according to claim 4, wherein the plant seed, the whole plant, the tissue portion, or the culture medium of the plant is treated with the plant growth regulator according to any one of claims 1 to 3. The application according to claim 5, wherein the treatment is a spraying treatment on a plant or a plant seed, or a spraying treatment or an irrigation treatment on the soil around the plant. The sixth aspect of claim 6 is characterized in that the spraying treatment on a plant refers to spraying on the foliage surface of the plant, and the spraying treatment on the plant seed refers to a dipping or coating treatment of the plant seed with a plant growth modifier. The application of the description. The application according to claim 7, wherein the timing of the spraying is before or during adversity stress of the plant. The application according to claim 4, wherein the application refers to an application of a plant growth regulator for improving stress tolerance of wheat, rice, corn or soybean. The application according to claim 4, wherein the stress resistance includes cold resistance, salt resistance, alkali resistance, and drought resistance.

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