WO2020181694A1

WO2020181694A1 - Plant growth regulator and application thereof

Info

Publication number: WO2020181694A1
Application number: PCT/CN2019/094501
Authority: WO
Inventors: 向文胜; 张继; 王相晶; 曹鹏
Original assignee: 东北农业大学
Priority date: 2019-03-13
Filing date: 2019-07-03
Publication date: 2020-09-17
Also published as: JP2022523241A; JP2024069371A; CN111685125A; JP7454587B2

Abstract

A plant growth regulator and an application thereof, relating to the technical field of agricultural science. In order to increase crop resistance, provided in the present invention is a plant growth regulator, the plant growth regulator being an aqueous solution comprising wugufengin, wugufengin being the active ingredient, and having a concentration of 0.5mg/L-200mg/L in the plant growth regulator; different application methods and concentrations are used according to the different growth stage of the plants, and the plant growth regulator can be used for seeds, whole plants, parts, or the cultivation medium of the crops, significantly enhancing the resistance of the crops, and promoting healthy plant growth.

Description

Plant growth regulator and its application

Technical field

The invention belongs to the field of agricultural science and technology, and specifically relates to a plant growth regulator and its application.

Background technique

In the production of crops and vegetables, abiotic stresses such as drought, salinity, and extreme temperatures are often encountered, which seriously affects the normal growth and development of plants, and eventually causes plant death and yield decline. On the basis of the improvement of plant varieties, technologies and planting patterns, the use of beneficial exogenous plant growth regulators has important economic significance for improving the stress resistance of plants. Wugufengin, also known as decoyinine and angustmycin A, is easily soluble in water, has good thermal stability and almost no cytotoxicity. The mice were injected with 2.5g/kg intraperitoneally without any side effects.

Summary of the invention

In order to improve the stress resistance of crops, the present invention provides a plant growth regulator, which is an aqueous solution containing Wugufengsu, wherein Wugufengsu is an effective ingredient and the concentration in the plant growth regulator is 0.5mg/L-200mg/L.

Preferably, the concentration of Wugufengsu in the plant growth regulator is 30 mg/L-100 mg/L.

More preferably, the concentration of Wugufengsu in the plant growth regulator is 50 mg/L.

The present invention also provides the application of the plant growth regulator in improving plant resistance to stress.

In a further limitation, the seeds, whole plants, tissue parts of the plant or the cultivation medium of the plant are treated with the above-mentioned plant growth regulator.

It is further defined that the treatment is a dispersal treatment to plants or plant seeds or a dispersal treatment to the soil around the plants or an irrigation treatment.

It is further defined that the dispersal treatment of the plant refers to spraying on the foliage of the plant; the dispersal treatment of the plant seeds refers to the soaking or coating treatment of the plant seeds with a plant growth regulator.

It is further defined that the timing of the spraying is before or during the stress of the plant.

In a further limitation, the application refers to the application of plant growth regulators in improving the stress resistance of wheat, rice, corn or soybeans.

Further defined, the stress resistance ability includes cold resistance, salt resistance, alkali resistance and drought resistance.

Beneficial effect

The invention aims to study the use method of using Wugufengsu to improve the stress resistance of crops, alleviate the damage of plants from drought, salinity and extreme temperature, promote plant growth and development, and increase the yield of crops and vegetables.

The application concentration of the Wugufengsu-containing plant growth regulator of the present invention is 0.5mg/L-200mg/L, preferably the concentration is 30mg/L-100mg/L, according to different growth stages of plants, different application methods and Concentration, which can be used for the seeds of plants (or crops), the whole plant, plant parts or the cultivation medium of the plant. The method of the present invention is easy to implement and easy to operate. As the effective ingredient, Wugufengsu can significantly enhance the crop’s It is resistant to stress and lasts for a long time, thereby promoting healthy plant growth.

Description of the drawings

Figure 1 shows the structure of Wugufengsu.

detailed description

The present invention will be further described in detail below in conjunction with the embodiments, but the content of the present invention is not limited.

The Wugufengsu of the present invention can be prepared through a microbial synthesis route: Streptomyces NEAU6 is fermented and cultured, and the culture solution is extracted to prepare the compound Wugufengsu. The Streptomyces sp. NEAU6 has been deposited in the General Microbiology Center of the China Microbial Culture Collection Management Committee on August 24, 2018, and the preservation address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, preservation number It is CGMCC 16338.

The media involved are as follows:

Gao's No. 1 Agar Medium: soluble starch 20g, NaCl 0.5g, KNO ₃ 1g, FeSO ₄ .7H ₂ O 0.01g, K ₂ HPO ₄ 0.5g, MgSO ₄ .7H ₂ O 0.5g, agar 20g, distilled water 1000ml , PH 7.2-7.4.

Seed culture medium: tryptone 17g, soy peptone 3g, NaCl 5g, K ₂ HPO ₄ 2.5g, glucose 2.5g, distilled water 1000ml, pH 7.2-7.4.

Fermentation medium: soluble starch 10g, glucose 10g, glycerol 10g, tryptone 5g, yeast powder 5g, CaCO ₃ 3g, tap water 1000ml, pH 7.2-7.4.

Under aseptic conditions, scrape the spores of strain NEAU6 that have been activated on the Gao's No. 1 agar medium and transfer them to the seed medium, culture at 30°C and 200 rpm for 2 days, and then transfer the seed liquid to 6% (v/v) ) Inoculated into fermentation medium, cultivated at 30°C and 200rpm for 5 days. The bacteria were removed by centrifugation, the supernatant was extracted 3-4 times with an equal volume of ethyl acetate, the extract was concentrated and then subjected to silica gel column chromatography, gel chromatography, C-18 column chromatography, and the compound was obtained after chromatography . The structure of the compound was confirmed by 1H-NMR, 13C-NMR, and MS spectrum analysis. The structural formula is shown in Figure 1.

The involved plant varieties, film-forming agent polyvinyl alcohol, dispersant sodium lignosulfonate, thickener xanthan gum, wetting agent fatty acid, and experimental utensils can all be purchased through commercial channels.

Example 1. Application of plant growth regulators in improving drought resistance of rice.

The plant growth regulator in this embodiment is an aqueous solution containing Wu Gu Feng Su, and the concentration of Wu Gu Feng Su in the plant growth regulator is 0.5 mg/L-200 mg/L.

The application of the plant growth regulator described in this embodiment in improving the drought resistance of rice means that rice seeds are soaked in an aqueous solution of Wugufengsu at a concentration of 0.5mg/L-200mg/L at room temperature, and the soaking time is It can be within 12-24 hours.

Specific steps are as follows:

(1) Weigh 50mg Wugufengsu, dissolve it with deionized water, dilute to 100mL, and prepare 500mg/L Wugufengsu mother liquor;

(2) Take different volumes of the mother liquor in step (1), add deionized water according to the proportion, and dilute them into a Wugufengsu aqueous solution with different concentrations between 0.5mg/L-200mg/L, and the concentrations are shown in Table 1;

(3) Choose rice Longjing 31 seeds with full and uniform grains, sterilize them with 5% sodium hypochlorite by volume percentage for 3 minutes, rinse them with distilled water 3 times, and soak the seeds with Wugufengsu aqueous solutions with different concentration gradients for 48 hours.

(4) The seeds of rice variety Longjing 31 were used to carry out a germination test under drought stress. The soaked seeds were transferred to a petri dish with a diameter of 9 cm. Each petri dish contained 16 rice seeds and repeated three times. Then add 10% mass percent PEG-6000 solution to each petri dish to simulate drought stress, change the PEG-6000 solution every 12h, observe the germination of rice seeds after 4 days, and count the root length and buds of rice long. The results are shown in Table 1.

Table 1. Effects of soaking seeds with different concentrations of Wugufengsu aqueous solution on germination of rice Longjing 31 seeds under drought stress

It can be seen from Table 1 that various concentrations of Wugufengsu aqueous solutions can improve the drought resistance of rice seeds. From the comparison of germination rate, root length and sprout length, it can be seen that using Wugufengsu to soak seeds can alleviate the drought stress of rice seeds and significantly increase the germination and growth of rice seeds, and it is the best at a concentration of 50mg/L, compared with the control , Shoot length and root length increased by 34.9% and 65.4%, respectively.

Example 2. Application of plant growth regulators in improving rice salt stress ability.

The application of the plant growth regulator described in this embodiment to improve the salt stress ability of rice refers to using 0.5 mg/L-200 mg/L Wugufengsu aqueous solution for root soaking treatment at room temperature.

Specific steps are as follows:

(2) Take different volumes of the mother liquor in step (1), add deionized water according to the proportion, and dilute them into a Wugufengsu aqueous solution with different concentrations between 0.5mg/L-200mg/L, and each concentration is shown in Table 2;

(3) The seeds of rice variety Longjing 31 with full and uniform grains were selected, disinfected with sodium hypochlorite at a concentration of 5% by volume for 3 minutes, rinsed with distilled water for 3 times, and soaked with distilled water.

(4) Pick uniform seeds and place them in a petri dish with a diameter of 9 cm and cultivate them with distilled water. When the seedlings grow to one leaf and one core, use nutrient solution to cultivate to two leaves and one core. Then the roots were soaked with different concentration gradients of Wugufengsu aqueous solution for 24h, and then 0.2mol/L sodium chloride solution was added for stress treatment, the sodium chloride solution was changed every 24h, and the plant height and root fresh weight of rice were measured after 3 days . The results are shown in Table 2.

Table 2. Effects of different concentrations of Wugufengsu aqueous solutions on the growth of rice Longjing 31 at seedling stage under salt stress

It can be seen from Table 2 that after soaking the roots of rice with different concentrations of Wugufengsu at the seedling stage, salt stress can enhance the salt resistance of rice. When the Wugufengsu concentration was 50mg/L, the effect was the best. Compared with the control, the plant height, fresh weight with seedlings and fresh root weight increased by 42.5%, 31.6% and 19.6% respectively.

Example 3. Application of plant growth regulators in improving the salt tolerance of rice.

The application of the plant growth regulator described in this embodiment in improving the salt tolerance of rice is specifically sprayed on the surface of 0.5mg/L-200mg/L Wugufengsu aqueous solution at room temperature, and the spraying amount is 0.1L/m ^2. Spray 2-3 times.

Specific steps are as follows:

(1) Weigh 50mg of Wugufengsu, dissolve it with deionized water, dilute to 100mL, and prepare 500mg/L Wugufengsu mother liquor;

(2) Take different volumes of the mother liquor in step (1), add deionized water according to the proportions, and dilute them into a Wugufengsu aqueous solution with different concentrations between 0.5mg/L-200mg/L, and the concentrations are shown in Table 3;

(4) Pick uniform seeds and place them in a petri dish with a diameter of 9 cm and cultivate them with distilled water. When the seedlings grow to one leaf and one core, use nutrient solution to cultivate to two leaves and one core. Then spray with different concentration gradients of Wugufengsu aqueous solution, spray once every 12h, spray 3 times, then add 0.2mol/L sodium chloride solution for stress treatment, replace sodium chloride solution every 24h, 3 The height and fresh weight of rice were measured after days. The results are shown in Table 3.

Table 3. Effects of different concentrations of Wugufengsu aqueous solution sprayed on the growth of rice Longjing 31 at the seedling stage under salt stress

It can be seen from Table 3 that after spraying rice with different concentrations of Wugufengsu aqueous solution at the seedling stage, salt stress can enhance the salt resistance of rice. When the Wugufengsu concentration was 50mg/L, the effect was the best. Compared with the control, the plant height, fresh seedling weight and root fresh weight increased by 46.1%, 27.7% and 19% respectively.

Example 4. Application of plant growth regulators in improving the alkali resistance of rice.

The application of the plant growth regulator described in this embodiment in improving the alkali resistance ability of rice is specifically sprayed on the leaf surface with 0.5mg/L-200mg/L Wugufengsu aqueous solution at room temperature, and the spraying amount is 0.1L/ m ² , spray 2-3 times.

Specific steps are as follows:

(2) Take different volumes of the mother liquor in step (1), add deionized water according to the proportion, and dilute them into a Wugufengsu aqueous solution with different concentrations between 0.5mg/L-200mg/L, and the concentrations are shown in Table 4;

(4) Pick uniform seeds and place them in a petri dish with a diameter of 9 cm and cultivate them with distilled water. When the seedlings grow to one leaf and one core, use nutrient solution to cultivate to two leaves and one core. Then spray with different concentration gradients of Wugufengsu aqueous solution, spray once every 12h, spray 3 times, then add 0.2mol/L sodium carbonate solution for stress treatment, replace sodium carbonate solution every 24h, and measure after 3 days The plant height of rice is fresh and heavy. The results are shown in Table 4.

Table 4. Effects of different concentrations of Wugufengsu aqueous solution sprayed on the growth of rice Longjing 31 at seedling stage under alkaline stress

It can be seen from Table 4 that after spraying rice with different concentrations of Wugufengsu aqueous solution at the seedling stage, alkali stress can enhance the alkali resistance of rice. When the Wugufengsu concentration was 50mg/L, the effect was the best. Compared with the control, the plant height, fresh seedling weight and root fresh weight increased by 13.1%, 33% and 30.1%, respectively.

Example 5. Application of plant growth regulators in improving corn resistance to cold.

The application of the plant growth regulator described in this embodiment to improve the cold resistance ability of corn is specifically to coat seeds with 0.5mg/L-200mg/L Wugufengsu aqueous solution at room temperature.

Specific steps are as follows:

(2) Take different volumes of mother liquor in step (1), film-forming agent polyvinyl alcohol 5%, dispersant sodium lignosulfonate 2%, thickener xanthan gum 1%, wetting agent fatty acid 1%, and the rest The amount is water, prepare Wugufengsu seed coatings with different concentrations between 0.5mg/L-200mg/L, and each concentration is shown in Table 5;

(3) Select the seeds of the corn variety Demeiya No. 1 with full and uniform grains, sterilize them with sodium hypochlorite at a concentration of 5% by volume for 3 minutes, rinse with distilled water 3 times, and dress the seeds with Wugufengsu seed coating agent. Let dry for later use;

(4) Before sowing, select corn seeds with complete seed coats, uniform sizes, and full grains, and sown them in a plastic box with a layer of wet sand at the bottom. Sow 10 seeds in each pot, covered with about 1 cm of wet sand, and place in light. Cultivate in an incubator at a temperature of 15°C, and measure the germination rate after 7 days.

Table 5. Effects of different concentrations of Wugufengsu seed coating agent on seed germination of maize variety Demeiya No. 1 under low temperature stress

The results show that different concentrations of Wugufengsu can improve the low temperature resistance of corn seeds. When the Wugufengsu concentrations were 0.5mg/L, 10mg/L, 30mg/L, 50mg/L, 100mg/L and 200mg/L, the germination rates were 23%, 43%, 61%, 72%, 70 % And 37%, significantly higher than the 20% germination rate of the control group.

Claims

A plant growth regulator, which is characterized in that the plant growth regulator is an aqueous solution containing Wu Gu Feng Su, wherein Wu Gu Feng Su is the effective ingredient, and the concentration in the plant growth regulator is 0.5 mg/L-200 mg/L .
The plant growth regulator according to claim 1, wherein the concentration of Wugufengsu in the plant growth regulator is 30 mg/L-100 mg/L.
The plant growth regulator according to claim 1, wherein the concentration of Wugufengsu in the plant growth regulator is 50 mg/L.
The use of the plant growth regulator according to any one of claims 1 to 3 in improving plant resistance to stress.
The application according to claim 4, characterized in that the seed, whole plant, tissue parts of the plant or the cultivation medium of the plant are treated with the plant growth regulator according to any one of claims 1-3.
The application according to claim 5, wherein the treatment is a dispersal treatment to plants or plant seeds, a dispersal treatment to the surrounding soil of the plants, or an irrigation treatment.
The application according to claim 6, characterized in that the dispersal treatment of the plants refers to spraying on the foliage of the plants; the dispersal treatment of the plant seeds refers to the use of plant growth regulators for soaking or encapsulating plant seeds衣处理。 Clothing processing.
The application according to claim 7, wherein the timing of the spraying is before or during the stress of the plant.
The application according to claim 4, characterized in that the application refers to the application of plant growth regulators in improving the stress resistance of wheat, rice, corn or soybean.
The application according to claim 4, wherein the stress resistance ability includes cold resistance, salt resistance, alkali resistance and drought resistance.