CN110558216B - Eustoma russellianum seedling growing method for eliminating rosette characters through temperature and light regulation - Google Patents
Eustoma russellianum seedling growing method for eliminating rosette characters through temperature and light regulation Download PDFInfo
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Abstract
The invention discloses a eustoma grandiflorum seedling culture method for eliminating rosette characters through temperature and light regulation, which solves the problems of seed dormancy of eustoma grandiflorum, seedling rosette and field planting flowering obstruction. The method comprises low-temperature moisture preservation pre-culture after sowing, light and temperature regulation and control in germination accelerating culture and strong seedling culture periods, low-temperature vernalization treatment of seedlings before outplanting and light regulation and control. The average germination rate of the seeds reaches 96.4%, the average rosette rate at the seedling stage is 0.8%, and the average rosette rate at the flowering and planting stage is 0.91%.
Description
Technical Field
The invention belongs to the technical field of fine variety breeding, and particularly relates to a temperature regulation and control method for eustoma grandiflorum seeds at different stages of seedling culture.
Background
Eustoma grandiflorum (Eustoma grandiflorum) is a perennial herb of genus Eustoma of family Gentianaceae, and its production amount is in the seventh world, and is a new flower species developed most rapidly in recent years in China following lily and China rose. The eustoma grandiflorum flower not only has the beauty of rose, but also has unique blue and multiple color systems, and is called porcelain rose because of long vase period. However, more than 60% of seedlings used in the current domestic flower production are imported from the Netherlands, the domestic seedling supply is less than 40%, the rosette rate after the domestic seedlings are planted is 5-8%, the rosette rate even reaches more than 20% in summer, the rosette rate of the domestic seedlings is basically not generated in the foreign imported seedlings, and the rosette rate in summer is not more than 2%.
Eustoma grandiflorum belongs to crops sensitive to temperature and illumination, seeds and seedlings of eustoma grandiflorum have dormancy characteristics, and low-temperature treatment is an important means for breaking dormancy. Therefore, the dormancy of seeds and seedlings is broken through by different temperature treatment in different stages during seed seedling raising, induction and occurrence of rosette in seedling stage and growth stage are prevented, at present, researches on temperature, illumination, period and the like required in different stages of seed seedling raising are not completely broken through, so that seed germination rate is low, growth vigor is irregular, the proportion of rosette seedlings is high, and the rosette problem in summer seedling raising and cut flower planting is more prominent. The 'lotus throne seedling' mainly shows that internode is shortened, leaves are widened, growth is slow or stopped, small seedlings with lotus thrones do not grow like 'lotus flowers' in the land, large seedlings in the field show that growth is slow, flowering is delayed, and the seedlings are poor in flowering and even do not bloom, so that the quality of seedlings and cut flowers is seriously influenced. The key technology influencing the occurrence of the rosette of the eustoma grandiflorum is researched, and the problem of solving and eliminating the occurrence of the rosette seedling is the main problem to be solved urgently in the production of the seedling and the cut flower at present.
Disclosure of Invention
In order to solve the technical problems, the invention provides a eustoma grandiflorum seedling growing method for eliminating rosette characters through temperature and light regulation, which comprises the following steps:
(1) low-temperature moisturizing and pre-cultivating after sowing: sowing eustoma grandiflorum seeds into seedling raising hole trays filled with self-prepared matrixes, spraying water, sealing the seedling raising hole trays with colorless transparent films to ensure that the seeds are in the wet matrixes, and then placing the seedling raising hole trays in a refrigerating chamber at the temperature of 6-10 ℃ for dark cultivation for 25-30 days;
the self-prepared matrix is prepared by the following method: mixing the turf, the perlite and the vermiculite according to the volume ratio of 8:1:1 to obtain a mixed matrix; adding 50 liters of self-prepared matrix fertilizer into each cubic meter of mixed matrix, and adding water to adjust the water content of the self-prepared matrix to be 40-50%; the specification of the turf is as follows: the pH value is 5.5-6.0, and the thickness is 1-10 mm; the self-prepared matrix fertilizer is prepared by dissolving 75 g of compound fertilizer, 10 g of calcium magnesium nitrate fertilizer and 20 g of 50% carbendazim wettable powder in 50 liters of water, wherein the compound fertilizer contains N15% w/w: p2O5 10％w/w：K2O15% w/w; the calcium nitrate magnesium fertilizer contains 8.0% w/w of nitrate nitrogen: ca 8.5% w/w Mg 1.8% w/w;
(2) accelerating germination and cultivating: taking the seedling culture hole tray in the step (1) out of the refrigerating chamber, putting the seedling culture hole tray into a greenhouse for accelerating germination and cultivation, firstly accelerating germination and cultivating for 5-8 days, removing a film for packaging the seedling culture hole tray after more than 40% of seeds germinate, and then continuously cultivating for 15-20 days to transfer to the next step of strong seedling cultivation when seedlings with 2 pairs of true leaves are formed; the conditions during the germination cultivation are as follows: the temperature in the greenhouse is 22-24 ℃, the illumination is 8000-10000 lx, and the relative air humidity is more than 80%, and the substrate in the seedling culture plug tray is kept moist by spraying water;
(3) strong seedling cultivation: adjusting the seedling environment conditions in a greenhouse, keeping the temperature in the greenhouse at 25-28 ℃, the relative humidity of air at 50-60%, the illumination intensity at 15000-25000 lx and the illumination time at 8-10 hours, spraying water to keep the self-prepared matrix in the seedling plug moist, culturing strong seedlings for 25-30 days, and transferring the seedlings to the low-temperature vernalization culture before the seedlings are taken out of the nursery of the next step when the seedlings grow to have 3-4 pairs of true leaves; during the strong seedling cultivation period, spraying the bactericide for 1-2 times, and spraying the self-prepared nutrient solution for 1-2 times; the interval time for spraying the self-prepared nutrient solution for 2 times is 8-10 days; the interval time for spraying the bactericide for 2 times is 10-12 days; the self-prepared nutrient solution is prepared by dissolving 250 g of macroelement water-soluble fertilizer, 30 g of chelated iron and 250 kg of water, wherein the macroelement water-soluble fertilizer contains N15% w/w: p2O515％w/w：K2O 15％w/w：MgO 1％w/w；
(4) Low-temperature vernalization cultivation before outplanting: adjusting the temperature in the greenhouse to be 16-20 ℃, the relative air humidity to be 50-60%, the illumination intensity to be 15000-20000 lx, the illumination time to be 8-10 hours, cultivating the plantlets for 10-14 days, planting after the plantlets are out of the nursery, and spraying the bactericide for 1 time in the low-temperature vernalization cultivation period before the plantlets are out of the nursery.
The bactericide in the step (3) and the step (4) is 70% thiophanate methyl wettable powder with 1200 times of liquid.
Compared with the prior art, the invention has the advantages that:
1. the invention greatly improves the germination rate and reduces the lotus throne rate in the seedling stage and the flowering and planting stage.
The method greatly improves the germination rate, reduces the rosette rate in the seedling stage and the flowering planting stage, solves the problem that seedlings and rosettes are prominent in seedling breeding and flowering planting of the eustoma grandiflorum seedlings, and improves the seedling and cut flower quality.
2. The method of the invention adopts low-temperature moisture-preserving pre-cultivation after sowing, improves the germination rate of the seedlings and reduces the occurrence of rosette in the seedling stage.
The eustoma grandiflorum seed dormancy method has certain dormancy characteristics, seed dormancy is broken through low-temperature moisture preservation and pre-cultivation of the sowed seeds, germination is promoted, and seedling growth is neat, the average germination rate reaches 96.4%, the average seedling-stage rosette rate is 0.8%, compared with the method that the seeds are directly placed into a seedling-raising greenhouse for cultivation after sowing, the germination rate is improved by 9.58%, and the average seedling-stage rosette rate is reduced by 78.1%.
3. The method of the invention adopts low-temperature vernalization treatment and illumination regulation before outplanting, so that the rosette incidence rate of the eustoma grandiflorum seedlings in the flowering and planting period after transplanting is obviously reduced.
As the eustoma grandiflorum plants are sensitive to temperature and light in growth and flowering, dwarfing, growth retardation or slow growth can occur after plantlets are planted to cause the rosette not to bloom, and the conditions of high temperature and poor light are the main reasons of plantlet dormancy. By carrying out low-temperature vernalization before auxiliary emergence of the seedlings, rosette occurrence in the planting period is reduced, and compared with the vernalization before nursery emergence, the rosette rate in the average flowering planting period is reduced from 7.8% to 0.91%, and the rosette rate in the average flowering planting period is reduced by 88.3%.
Detailed Description
EXAMPLE 1 Process according to the invention
(1) Sowing: sowing eustoma grandiflorum seeds into seedling raising hole trays filled with self-prepared matrixes manually or mechanically, sowing 1 seed in each hole of 200 holes of the seedling raising hole trays, spraying water after sowing, and sealing the seedling raising hole trays with colorless transparent films to ensure that the seeds are in the wet matrixes; the self-prepared matrix is prepared by the following method: mixing the turf, the perlite and the vermiculite in a volume ratio of 8:1:1 to obtain a mixed matrix, adding 50 liters of self-prepared matrix fertilizer into each cubic meter of the mixed matrix, mixing to obtain a self-prepared matrix, adding water to adjust the water content of the self-prepared matrix to be 40-50%, wherein the turf is prepared by the following specifications: the pH value is 5.5-6.0, and the thickness is 1-10 mm; the self-prepared matrix fertilizer is prepared by dissolving 75 g of compound fertilizer, 10 g of calcium magnesium nitrate fertilizer and 20 g of 50% carbendazim wettable powder in 50 liters of water, wherein the compound fertilizer contains N15% w/w: p2O5 10％w/w：K2O15% w/w, and the calcium magnesium nitrate fertilizer contains nitrate Nitrogen (NO)3-N)8.0％w/w：Ca 8.5％w/w:Mg 1.8％w/w。
(2) Low-temperature moisturizing and pre-cultivating after sowing: and (2) placing the seedling raising hole trays which are sowed and packaged in the step (1) into a refrigerating chamber at the temperature of 8 ℃ for dark cultivation, carrying out dormancy breaking treatment on the seeds, cultivating for 28 days, and then transferring to the next step of germination accelerating cultivation.
(3) Accelerating germination and cultivating: and (3) taking the seedling raising hole trays which are sowed in the step (2) and pre-cultivated at low temperature in a moisture-preserving manner out of a refrigerating chamber, putting the seedling raising hole trays into a greenhouse for accelerating germination cultivation, firstly accelerating germination cultivation for 5-8 days, removing a film for packaging the seedling raising hole trays after more than 40% of seeds germinate, and continuously cultivating for 15-20 days to transfer to the next step of strong seedling cultivation when seedlings with 2 pairs of true leaves are formed. The conditions during the whole germination accelerating cultivation period are as follows: the temperature in the greenhouse is 22-24 ℃, the illumination is 8000-10000 lx, and the relative air humidity is more than 80%, and the substrate in the seedling culture plug tray is kept moist by spraying water.
(4) Strong seedling cultivation: adjusting the environmental conditions of seedling cultivation in a greenhouse, keeping the temperature in the greenhouse at 25-28 ℃, keeping the relative humidity of air at 50-60% (when the humidity is too high, the humidity can be reduced by windowing or air draft), keeping the illumination intensity at 15000-25000 lx, keeping the illumination time for 8 hours, supplementing light when the illumination is insufficient, spraying water to keep a self-prepared matrix in a seedling cultivation hole tray moist, cultivating strong seedlings for 25-30 days, and transferring the seedlings to the next low-temperature vernalization cultivation before the seedlings are grown to have 3-4 pairs of true leaves; during the period of strong seedling cultivation, self-prepared nutrient solution is sprayed for 2 times and bactericide is sprayed for 1 time according to the growth vigor of the seedlings; the interval time for spraying the self-prepared nutrient solution for 2 times is 10 days; the self-prepared nutrient solution is prepared by dissolving 250 g of macroelement water-soluble fertilizer, 30 g of chelated iron and 250 kg of water, wherein the macroelement water-soluble fertilizer contains N15% w/w: p2O5 15％w/w：K2O15% w/w: MgO 1% w/w. The bactericide is 70% thiophanate methyl wettable powder with 1200 times of liquid.
(5) Low-temperature vernalization cultivation before outplanting: adjusting the temperature in a greenhouse to be 16-20 ℃, keeping the relative air humidity at 50-60%, illuminating intensity at 15000-20000 lx, illuminating for 10 hours, cultivating seedlings for 12 days, outplanting and planting, and spraying a bactericide for 1 time during the cultivation period, wherein the bactericide is 70% thiophanate-methyl wettable powder of 1200-time liquid.
Example 2 comparative experiment:
the following comparative tests were carried out according to the method of example 1 using eustoma grandiflorum main cultivars 'whine purple' and 'venus powder edge' as materials:
comparative test a: the influence of different temperatures and time of moisture preservation and pre-cultivation after sowing on the seed germination.
Comparative test B: influence of low-temperature vernalization cultivation before outplanting on rosette after seedling planting.
Comparative test C: compared with the prior art, the method has the influence on the lotus throne at the seedling stage and after the seedling is planted (at the flowering planting stage).
The key link of breaking dormancy in eustoma grandiflorum seedling culture is verified by a contrast test, and further analysis verifies that the method achieves the purposes of improving the germination rate and reducing the occurrence of rosette in the seedling stage and after seedling planting (flowering planting stage) by regulating and controlling the culture temperature, illumination and time in each stage of low-temperature moisturizing pre-culture, germination accelerating culture, strong seedling culture and low-temperature vernalization culture before outplanting.
And (3) seedling stage: refers to the period from sowing to outplanting in example 1.
And (3) flowering and planting period: the seedling of eustoma grandiflorum is planted until the eustoma grandiflorum can be used as the period for collecting fresh cut flowers.
Comparative test a: effect of post-sowing moisturizing Pre-incubation on seed germination at different temperatures and times (Table 1)
Test materials: 'Venus powder edge' and 'resonant violet'.
Pre-culturing: the temperatures of moisturizing and pre-cultivating after sowing are respectively as follows: culturing at 2 deg.C, 6 deg.C, 10 deg.C, 14 deg.C for 20 days, 25 days, 30 days, and 35 days in dark in a refrigerator.
Comparison: the moisture-keeping culture temperature after seeding is the conventional temperature in a greenhouse during seeding, and the cultivation is carried out in a dark way in a refrigerating chamber at the temperature of 18-23 ℃.
And (3) counting the germination rate: and (5) culturing for 20 days to count the germination rate of the seeds.
The results are shown in Table 1:
1. the germination rate of the seeds subjected to low-temperature moisture preservation pre-culture treatment after sowing is higher than that of a control, the average germination rate of all the treated seeds is 95.4%, and the average germination rate is improved by 7.5% compared with that of the control 87.9% (95.4-87.9-7.5).
2. Considering that the temperature reduction cost is increased due to the fact that the temperature is too low or the culture time is prolonged, the pre-culture temperature is 6-10 ℃, the culture time is 25-30 days, the average germination rate of the optimized treatment in the period reaches 96.35%, and the average germination rate is improved by 8.42% compared with that of a control 87.93% (96.35-87.93 is 8.42). The improvement rate was 9.58% (sprout improvement rate [ (96.35-87.93)/87.93] × 100%: 9.58%).
Table 1: the pre-cultivation at different temperatures and time of moisture retention affect the germination rate unit of the seeds: is based on
Note: the germination rate is the number of germination seeds/number of sowing seeds. And (4) germination judgment standard: visible true leaves are formed.
Comparative test B: effect of Low-temperature vernalization cultivation before outplanting on occurrence of rosette after seedling planting (Table 2)
In order to solve the serious problem of rosette occurrence in flowering planting, a test that the seedling-stage low-temperature treatment breaks dormancy of eustoma grandiflorum seedlings is carried out, and meanwhile, small seedlings with 3-4 pairs of true leaves obtained by strong seedling cultivation in the step (4) in the embodiment 1 are subjected to low-temperature vernalization cultivation before outplanting: under the conditions that the air relative humidity in a greenhouse is 50-60%, the illumination intensity is 15000-20000 lx and the illumination time is 8 hours, five treatments of 12 ℃, 16 ℃, 20 ℃, 24 ℃ and 28 ℃ are set in the greenhouse, seedlings are cultivated for 12 days under each temperature treatment and are outplanted, and 70% thiophanate-methyl wettable powder with the concentration being 1200 times is sprayed for 1 time at each temperature treatment in the same time, so that the disease occurrence is prevented. And (4) planting the seedlings after the seedlings are out of the nursery, planting the seedlings according to a conventional cut flower cultivation method of eustoma grandiflorum till the eustoma grandiflorum can be used as a fresh cut flower for collection, and counting the rosette rate in the period.
Comparison: the conditions were the same as the above five treatments except that the temperature of the pre-treatment before leaving the nursery was 32 ℃.
In the flowering and planting period, the proportional occurrence number of the lotus throne seedlings is counted 50 days after planting (see table 2). From the test results it can be seen that: the occurrence rate of the rosette in the later planting (flowering planting period) can be effectively broken through the 'low-temperature vernalization cultivation before outplanting' in the seedling stage, and the effect of reducing the rosette occurrence is obviously increased along with the reduction of the temperature. The effect is best at 12-20 ℃, the average rosette incidence rate is 1.41%, the rosette incidence rate is reduced by 4.64 percentage points compared with 6.05%, the reduction rate is 76.7% (the rosette reduction rate is [ (1.41-6.05)/6.05] × 100% -76.7%), when the temperature exceeds 24 ℃, the rosette average reduction rate is less than 1 percentage point, and when the temperature exceeds 28 ℃, the rosette average reduction rate is only 0.4 percentage point. In the experiment, the treatment effect at 12 ℃ is the best, but in the actual large-scale seedling production, the temperature of 16-20 ℃ is selected as the most economical and effective low-temperature vernalization cultivation temperature before outplanting in consideration of the high greenhouse cooling cost in summer.
Table 2: influence of low-temperature vernalization cultivation before outplanting on lotus seed seats after cultivation
Note: judging 'rosette' in the flowering and planting period: and taking the condition that the bolting is not performed 45-50 days after planting as a standard.
Comparative test C: compared with the prior art, the method of the invention has the influence on the lotus throne at the seedling stage and after planting (Table 3, Table 4)
The varieties of 'Venus powder edge' and 'whining purple' are taken as materials to carry out a system verification test.
The invention has the following verification tests: the steps are the same as the example 1 except that the following steps are different, the temperature in the low-temperature moisture-preserving pre-culture after the sowing in the step (2) is 6 ℃ and 10 ℃, and the pre-culture time is 25 days; and (5) before outplanting, the temperature in the low-temperature vernalization cultivation is 16 ℃ and 20 ℃, and the vernalization cultivation time is 12 days.
Comparison: after sowing according to the step (1) in the example 1, the germination accelerating cultivation and the strong seedling cultivation (namely, the low-temperature moisture preservation pre-cultivation after sowing in the step (2) in the example 1 and the low-temperature vernalization cultivation before outplanting in the step (5)) in the step (3) in the example 1 are directly carried out, and the cultivation is carried out under the natural condition of more than 28 ℃ before outplanting.
The statistical comparison and analysis of the lotus throne seedlings are respectively carried out in the seedling stage and the flowering planting stage, and the effectiveness of the method is further verified.
The test results in the following tables 3 and 4 show that:
1. the average rosette rate of the method in the seedling stage (outplanting) is 0.8%, and compared with the rosette rate of 3.65% in the contrast average seedling stage, the rosette rate is reduced by 2.85%, and the reduction rate is 78.1%.
2. The rosette rate of the seedlings cultivated by the method in the flowering planting period after the seedlings are transplanted is greatly reduced, the average rosette rate in the flowering planting period is 0.91%, and compared with the control of 7.8%, the rosette rate is reduced by 6.89%, and the reduction rate is 88.3%.
3. The method can obviously reduce the lotus throne rate in the seedling stage and the flowering and planting stage.
Table 3: the method of the invention has the following effects on reducing the lotus throne in the seedling stage: (Lotus seed rate Unit:%)
Note: rosette rate [ rosette plant number/total seedling number ]. times.100%
And (3) judging lotus throne in the seedling stage: counting when the seedlings are out of nursery, 3-4 pairs of true leaves of the seedlings are out of nursery, the leaves are planted on the stems in clusters, and obvious internodes do not exist.
Counting time of lotus throne rate in seedling stage: when the seedlings are out of nursery.
The reduction rate of the lotus throne rate at the seedling stage of the invention is [ (the lotus throne rate at the seedling stage of the invention-the lotus throne rate at the contrast seedling stage)/the lotus throne rate at the contrast seedling stage ] × 100%.
TABLE 4 Effect of the method of the present invention on reducing rosette at flowering planting stage (rosette rate unit:%)
Note: rosette rate [ rosette plant number/total seedling number ]. times.100%
Judging 'rosette' in the flowering and planting period: and taking the non-bolting state at the statistical time of 45-50 days after planting as a standard.
Counting time of the lotus throne rate in the flowering and planting period: counting 50 days after the seedlings are out of the nursery and planted.
The reduction rate of the lotus throne rate in the flowering and planting period of the invention is [ (the lotus throne rate in the flowering and planting period of the invention-the lotus throne rate in the control flowering and planting period)/the lotus rate in the control flowering and planting period ] × 100%.
The embodiment shows that the method can obviously improve the seed germination rate, reduce the occurrence of the lotus throne seedlings in the seedling stage and the flowering planting stage, improve the quality of the seedlings and the cut flowers and achieve the effect of improving the quality of the seedlings and the cut flowers, and is suitable for large-scale production of different types of eustoma grandiflorum seedlings.
Claims (2)
1. A eustoma grandiflorum seedling growing method for eliminating rosette characters through temperature and light regulation is characterized in that:
(1) low-temperature moisturizing and pre-cultivating after sowing: sowing eustoma grandiflorum seeds into seedling raising hole trays filled with self-prepared matrixes, spraying water, sealing the seedling raising hole trays with colorless transparent films to ensure that the seeds are in the wet matrixes, and then placing the seedling raising hole trays in a refrigerating chamber at the temperature of 6-10 ℃ for dark cultivation for 25-30 days;
the self-prepared matrix is prepared by the following method: mixing the turf, the perlite and the vermiculite according to the volume ratio of 8:1:1 to obtain a mixed matrix; adding 50 liters of self-prepared matrix fertilizer into each cubic meter of mixed matrix, and adding water to adjust the water content of the self-prepared matrix to be 40-50%; the specification of the turf is as follows: the pH value is 5.5-6.0, and the thickness is 1-10 mm; the self-prepared matrix fertilizer is prepared by dissolving 75 g of compound fertilizer, 10 g of calcium magnesium nitrate fertilizer and 20 g of 50% carbendazim wettable powder in 50 liters of water, wherein the compound fertilizer contains N15% w/w: p2O5 10％w/w：K2O15% w/w; the calcium nitrate magnesium fertilizer containsNitrate nitrogen 8.0% w/w: ca 8.5% w/w Mg 1.8% w/w;
(2) accelerating germination and cultivating: taking the seedling culture hole tray in the step (1) out of the refrigerating chamber, putting the seedling culture hole tray into a greenhouse for accelerating germination and cultivation, firstly accelerating germination and cultivating for 5-8 days, removing a film for packaging the seedling culture hole tray after more than 40% of seeds germinate, and then continuously cultivating for 15-20 days to transfer to the next step of strong seedling cultivation when seedlings with 2 pairs of true leaves are formed; the conditions during the germination cultivation are as follows: the temperature in the greenhouse is 22-24 ℃, the illumination is 8000-10000 lx, and the relative air humidity is more than 80%, and the substrate in the seedling culture plug tray is kept moist by spraying water;
(3) strong seedling cultivation: adjusting the seedling environment conditions in a greenhouse, keeping the temperature in the greenhouse at 25-28 ℃, the relative humidity of air at 50-60%, the illumination intensity at 15000-25000 lx and the illumination time at 8-10 hours, spraying water to keep the self-prepared matrix in the seedling plug moist, culturing strong seedlings for 25-30 days, and transferring the seedlings to the low-temperature vernalization culture before the seedlings are taken out of the nursery of the next step when the seedlings grow to have 3-4 pairs of true leaves; during the strong seedling cultivation period, spraying the bactericide for 1-2 times, and spraying the self-prepared nutrient solution for 1-2 times; the interval time for spraying the self-prepared nutrient solution for 2 times is 8-10 days; the interval time for spraying the bactericide for 2 times is 10-12 days; the self-prepared nutrient solution is prepared by dissolving 250 g of macroelement water-soluble fertilizer, 30 g of chelated iron and 250 kg of water, wherein the macroelement water-soluble fertilizer contains N15% w/w: p2O515％w/w：K2O 15％w/w：MgO 1％w/w；
(4) Low-temperature vernalization cultivation before outplanting: adjusting the temperature in the greenhouse to be 16-20 ℃, the relative air humidity to be 50-60%, the illumination intensity to be 15000-20000 lx, the illumination time to be 8-10 hours, cultivating the plantlets for 10-14 days, planting after the plantlets are out of the nursery, and spraying the bactericide for 1 time in the low-temperature vernalization cultivation period before the plantlets are out of the nursery.
2. The eustoma grandiflorum seedling raising method for eliminating rosette traits through temperature and light regulation as claimed in claim 1, wherein the bactericide in the step (3) and the step (4) is 70% thiophanate methyl wettable powder with 1200 times of liquid.
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