CN115088489A - Eustoma russellianum vegetative propagation seedling method - Google Patents
Eustoma russellianum vegetative propagation seedling method Download PDFInfo
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- CN115088489A CN115088489A CN202210593793.7A CN202210593793A CN115088489A CN 115088489 A CN115088489 A CN 115088489A CN 202210593793 A CN202210593793 A CN 202210593793A CN 115088489 A CN115088489 A CN 115088489A
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- 241000511009 Eustoma exaltatum subsp. russellianum Species 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000005520 cutting process Methods 0.000 claims abstract description 42
- 241000196324 Embryophyta Species 0.000 claims abstract description 22
- 238000003306 harvesting Methods 0.000 claims abstract description 19
- 230000001902 propagating effect Effects 0.000 claims abstract description 3
- 239000000758 substrate Substances 0.000 claims description 15
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 7
- 238000005286 illumination Methods 0.000 claims description 6
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 4
- 238000007598 dipping method Methods 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 4
- 239000003595 mist Substances 0.000 claims description 4
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 3
- 244000060011 Cocos nucifera Species 0.000 claims description 3
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 3
- 239000003415 peat Substances 0.000 claims description 3
- 239000010451 perlite Substances 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- 238000005057 refrigeration Methods 0.000 claims description 3
- 230000000717 retained effect Effects 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims 2
- 230000037431 insertion Effects 0.000 claims 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 abstract description 7
- 230000000644 propagated effect Effects 0.000 abstract description 5
- 238000004904 shortening Methods 0.000 abstract description 2
- 230000007193 modulation by symbiont of host erythrocyte aggregation Effects 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 4
- 210000005069 ears Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000010903 husk Substances 0.000 description 2
- 240000006497 Dianthus caryophyllus Species 0.000 description 1
- 235000009355 Dianthus caryophyllus Nutrition 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 235000000100 Hibiscus rosa sinensis Nutrition 0.000 description 1
- 241000234435 Lilium Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 240000008254 Rosa chinensis Species 0.000 description 1
- 235000000664 Rosa chinensis Nutrition 0.000 description 1
- 235000016785 Rosa della China Nutrition 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000004017 vitrification Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a method for asexually propagating eustoma grandiflorum seedlings, which comprises the following steps: step one, planting of the scion-picking female parent plant; step two, cutting and harvesting; step three, cuttage; managing after cuttage; and step five, seedling hardening. The seedlings bred by the method have the following remarkable advantages: (1) the seedling period is shortened from 3 months to 18-21 days. (2) Shortening the growth period: the seedlings propagated by the method have developed root systems, short seedling revival period after planting, strong seedlings, quick growth, regular flowering and capability of being harvested after being planted for 12 weeks, and the circulation speed of the land is greatly increased.
Description
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a method for vegetative propagation of eustoma grandiflorum seedlings.
Background
The eustoma grandiflorum is commonly called as thornless rose, is rich in flower color, beautiful in flower shape, long in preservation period and popular with people. The Yunnan eustoma grandiflorum industry is rapidly developed, the planting area accounts for more than 70% of the whole country, the eustoma grandiflorum becomes the fourth big cut flower after China rose, lily and carnation, and the eustoma grandiflorum becomes the third big cut flower rapidly according to the growth rate in recent years. The planting area of Yunnan eustoma grandiflorum in 2021 year reaches 1.5 mu of mu, the annual output reaches 6 hundred million, the output value reaches 12 hundred million yuan, and the annual average increase exceeds 30%. In recent years, eustoma grandiflorum is one of the most vigorous and most potential-developed cut flower types from market conditions and production benefits of the eustoma grandiflorum.
However, the development of eustoma grandiflorum industry core sources and techniques have not been fundamentally solved.
The reason is as follows:
china depends on 100% of Eustoma russellianum varieties to be imported from Japan, and 70% of seedlings depend on the Netherlands to be imported. At present, F1 hybrid seeds are adopted for breeding eustoma grandiflorum seedlings, the sources of eustoma grandiflorum seedlings in China mainly have 2 ways, one way is imported from a Dutch professional breeding company, and the imported Dutch volume accounts for about 50% of the total demand volume. The production mode of the Dutch seedlings is as follows: seeds are purchased from Japanese breeding companies, specialized production of seedlings is carried out by using advanced equipment and technology, and then the seedlings are sold to all parts of the world. Secondly, local enterprises purchase Japanese seeds for self-breeding, and the seeds account for about 45% of the total demand. The F1 generation coated hybrid is adopted for breeding, the price is high, the price of each plant is 0.6-1.0 yuan, the transportation cost accounts for about 30%, the seeds germinate and the seedlings grow slowly, 3 months are needed from sowing to field planting, the environmental influence is great during the period, the seedling technical requirement is high, the seedling supply is limited, and the commercial production of eustoma grandiflorum cut flowers in China is greatly hindered.
The propagation by clone (mainly cutting propagation) is an important way for seedling production, and no commercial asexual propagation seedling of eustoma grandiflorum is available at home and abroad at present. A small number of research units carry out seedling propagation technical research on eustoma grandiflorum by adopting a tissue culture or cutting propagation mode, but the problems of variation and vitrification of tissue culture seedlings are prominent, the propagation efficiency is low, the problems of rosette, non-flowering and delayed flowering after the tissue culture seedlings are planted occur sometimes, and the large-scale application of the tissue culture seedlings is less. The cuttage propagation is greatly influenced by the quality of female parent, season and environmental conditions, the rooting rate is unstable, the seedling root rot in summer and the diseases and the flowering are serious after the planting, and the method is not popularized and applied.
The eustoma grandiflorum is very easy to have a rosette phenomenon (the eustoma grandiflorum is dense in leaves, does not bolt and does not grow tall), once the rosette phenomenon occurs, the rosette phenomenon is difficult to improve into a normal plant in a short period, the rosette phenomenon can be properly removed by technical means such as gibberellin spraying, but the flowering time can be delayed for 3-4 months, and huge loss is brought to a grower. In the actual production of eustoma grandiflorum, about 30% of seedlings produced by seeds subjected to low-temperature cold treatment still have rosette phenomenon. Rosetting is currently considered to be a growth state caused by adverse environments, and rosetting may occur in different periods of growth of eustoma grandiflorum. It is currently widely believed that either too high or too low a temperature causes rosetting, but the threshold range of temperature is not precisely defined, but there is also literature indicating that rosetting is caused at night temperatures above 23 ℃ and at night temperatures below 8 ℃. In conclusion, research on providing a propagation method for reducing rosette formation of eustoma grandiflorum is a technical problem to be solved.
Disclosure of Invention
The invention provides a method for asexual propagation of eustoma grandiflorum seedlings, which shortens the seedling culture period of eustoma grandiflorum, improves the rooting rate of seedlings, reduces the rosette percentage of seedlings, improves the later growth and flowering consistency of seedlings, and provides basis and technical support for the industrial production of high-yield and high-quality seedlings.
The invention is realized by adopting the following technical scheme.
The invention relates to a method for asexually propagating eustoma grandiflorum seedlings, which comprises the following steps:
step one, establishing and planting of the scion-picking female parent plant: planting 4-leaf and 1-heart eustoma grandiflorum seedlings in a scion-picking female parent greenhouse.
The planting substrate adopts a light soilless culture substrate coconut husk; the female parent cultivation greenhouse is controlled between 13 ℃ and 28 ℃, the illumination intensity is between 30000 and 50000Lx, and the relative air humidity is between 50 percent and 70 percent.
And when the plant grows to 6 leaves and 1 heart, removing the terminal bud to promote the lateral bud to sprout at the base of the leaf, and when the lateral bud grows to 4 leaves and 1 heart, removing the terminal bud.
After multiple topping and pinching, the number of the picking ears of the female parent is relatively stable after 6-8 weeks, the yield of the female parent reaches 2-3 cutting ears/two weeks/plant, the planting of the female parent is finished, and the seedling production is formally carried out.
Step two, cutting and harvesting: selecting strong lateral buds from the established eustoma grandiflorum female parent plant, and harvesting cuttings with sterilized scissors.
Each cutting retained 2 leaves 1 heart or 4 leaves 1 heart.
And (3) after cutting and harvesting, putting into a cutting and harvesting bag in time, spraying water in time to preserve moisture, putting into a refrigeration house with the temperature of 4-6 ℃ for standby, wherein the time is preferably not more than 24 hours, or immediately harvesting and inserting.
Step three, cuttage:
preparing a seedling culture substrate: a72-mesh plug tray is adopted, and a seedling substrate block is placed in the plug tray.
The matrix block is made of peat: the perlite is mixed according to the volume ratio of 5: 1.
Before cutting, the substrate block fully absorbs water for later use.
Cuttage: dipping the base of the cutting into rooting powder, inserting the cutting into a seedling raising block, and inserting the cutting to the depth of the base of the leaf.
The rooting powder is prepared by uniformly mixing IBA (indolebutyric acid) in talcum powder, wherein the concentration of the IBA is 2500 ppm.
Step four, managing after cuttage:
the full light treatment is adopted, the air humidity in the cuttage greenhouse is kept above 85% through high-pressure mist intermittent spraying, and the whole-day temperature of the seedling raising greenhouse is kept at 24 ℃ through comprehensive regulation and control of heating pipes, fans, water curtains and other facilities.
And 5-7 days after cutting, callus appears at the base of the cutting shoot.
After 10-14 days, the root system rapidly grows and extends out of the seedling raising block, and the seedling begins to enter a seedling hardening greenhouse for hardening seedlings.
Step five, seedling hardening
During seedling exercising, the temperature is controlled between 18 ℃ and 21 ℃, the humidity is controlled between 70 percent and 85 percent, and the light is controlled at 20000-25000 Lx.
Hardening the seedlings for 5-7 days, leading the height of the seedlings to reach 6-10cm, and germinating.
The invention has the beneficial effects that: 1) the invention adopts full-light mist cuttage, and shortens the seedling culture period to 18-21 days by accurately controlling key environmental factors influencing cuttage rooting, such as temperature, humidity, illumination and the like, the rooting rate reaches more than 90%, the root system is developed, the seedling rate after transplanting is more than 95%, the seedling rate is obviously higher than that of a seed seedling, and the annual production is realized. 2) The invention solves the problems that root diseases are easy to infect by the conventional cuttage eustoma grandiflorum, the rooting rate is low, the rooting rate is unstable, the influence by seasons is large, and the commercial seedling production is difficult to realize. 3) Compared with the seed seedling, the seedling bred by the method has the following remarkable advantages: (1) the seedling period is shortened from 3 months to 18-21 days. (2) Shortening the growth period: the seedlings propagated by the method have developed root systems, short seedling revival period after planting, strong seedlings, quick growth, regular flowering and capability of being harvested after being planted for 12 weeks, and the circulation speed of the land is greatly increased. (comparing the average farmer planting time between 4 months and 6 months)
The invention is further explained below with reference to the drawings and the detailed description.
Drawings
FIG. 1 is a drawing of a eustoma grandiflorum ear picking female parent entity of the invention;
FIG. 2 is a diagram of vegetative propagation seedling of Eustoma russellianum of the present invention;
FIG. 3 is a diagram of a growing condition of Eustoma russellianum of the present invention;
FIG. 4 is a diagram of a blooming state of Eustoma grandiflorum of the present invention;
FIG. 5 is a first comparison of Eustoma grandiflorum real estate bred without the method of the present invention;
FIG. 6 is a second comparison chart of Eustoma grandiflorum species propagated without the method of the present invention;
Detailed Description
Step one, establishing and planting of ear picking female parent plants: planting the eustoma grandiflorum seedlings with 4 leaves and 1 heart in a harvest mother greenhouse.
The planting substrate adopts a light soilless culture substrate coconut husk; the female parent cultivation greenhouse is controlled between 13 ℃ and 28 ℃, the illumination intensity is between 30000 and 50000Lx, and the relative air humidity is between 50 percent and 70 percent.
And when the plant grows to 6 leaves and 1 heart, removing the terminal bud to promote the lateral bud to sprout at the base of the leaf, and when the lateral bud grows to 4 leaves and 1 heart, removing the terminal bud.
After multiple topping and pinching, the number of the picking ears of the female parent is relatively stable after 6-8 weeks, the yield of the female parent reaches 2-3 cutting ears/two weeks/plant, the planting of the female parent is finished, and the seedling production is formally carried out.
Step two, cutting and harvesting: selecting strong lateral buds from the planted eustoma grandiflorum female parent plants, and harvesting cuttings by using sterilized scissors.
Each cutting retained 2 leaves 1 heart or 4 leaves 1 heart.
And (3) after cutting and harvesting, putting into a cutting and harvesting bag in time, spraying water in time to preserve moisture, putting into a refrigeration house with the temperature of 4-6 ℃ for standby, wherein the time is preferably not more than 24 hours, or immediately harvesting and inserting.
Step three, cuttage:
preparing a seedling culture substrate: a72-mesh plug tray is adopted, and a seedling substrate block is placed in the plug tray.
The matrix block is made of peat: the perlite is mixed according to the volume ratio of 5: 1.
Before cutting, the substrate block fully absorbs water for later use.
Cuttage: dipping the base of the cutting into rooting powder, inserting the cutting into a seedling raising block, and inserting the cutting to the depth of the base of the leaf.
The rooting powder is prepared by uniformly mixing IBA (indolebutyric acid) in talcum powder, and the IBA concentration is 2500 ppm.
Step four, managing after cuttage:
the full light treatment is adopted, the air humidity in the cuttage greenhouse is kept above 85% through high-pressure mist intermittent spraying, and the whole-day temperature of the seedling raising greenhouse is kept at 24 ℃ through comprehensive regulation and control of heating pipes, fans, water curtains and other facilities.
And 5-7 days after cutting, callus appears at the base of the cutting shoot.
After 10-14 days, the root system rapidly grows and extends out of the seedling raising block, and the seedling begins to enter a seedling hardening greenhouse for hardening seedlings.
Step five, hardening off the seedlings
During seedling exercising, the temperature is controlled to be between 18 and 21 ℃, the humidity is controlled to be between 70 and 85 percent, and the light is controlled to be 20000-25000 Lx.
Hardening the seedlings for 5-7 days, leading the height of the seedlings to reach 6-10cm, and germinating.
By comparing the plant height and the flower number of the conventional seed seedling, the cuttage seedling propagated by the invention has almost no difference from the seed seedling in plant height and flower number, and has obvious advantages.
TABLE 1 statistics of the plant heights of cuttage seedlings of different eustoma grandiflorum varieties
TABLE 2 statistics of the number of flowers of different varieties of cuttage seedlings of Eustoma russellianum
According to some literature documents: methods for avoiding rosette formation include 1) when seed seedling is adopted, the seeds are treated by low-temperature vernalization; 2) The extremely high temperature and the low temperature are avoided in the planting process; 3) avoid the use of seeds harvested at high temperatures (>28 ℃).
The research of the invention finds that the relation between the eustoma grandiflorum rosette and the environment control and nutrients, including temperature, moisture, illumination, fertilizer and the like, between the balanced growth and the environment control is avoided by combining the eustoma grandiflorum with different growth periods, and the temperature is the key point. Long-term experiments prove that the eustoma grandiflorum planted in the base of the invention is observed, and the technical points in the steps of the application are kept: sufficient water supply, proper illumination and the like, and no rosette appears even if the temperature reaches 30 ℃.
As can be seen from a comparison of fig. 1 to 6, eustoma grandiflorum propagated by the method of the present invention has significant advantages, and particularly, the rosetting phenomenon is hardly seen. Therefore, the technical method of the invention is very significant in avoiding rosetting.
The above description is only a part of the specific embodiments of the present invention (since the formula of the present invention includes the numerical range, the embodiments are not exhaustive, the protection scope of the present invention includes the numerical range of the present invention and other technical essential ranges), and the detailed contents or common general knowledge known in the schemes are not described too much. It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and all technical solutions obtained by means of equivalent substitution or equivalent transformation are within the protection scope of the present invention for those skilled in the art. The scope of the claims of the present application shall be defined by the claims, and the detailed description and the like in the specification shall be used for explaining the contents of the claims.
Claims (10)
1. A method for asexually propagating eustoma grandiflorum seedlings is characterized by comprising the following steps: step one, planting the ear picking female parent plant; step two, cutting and harvesting; step three, cuttage; managing after cuttage; and step five, seedling hardening.
2. The method for asexual propagation of seedlings of eustoma grandiflorum according to claim 1, wherein the step of planting the scion female parent plant comprises: planting 4-leaf 1-heart eustoma grandiflorum seedlings in a planting matrix in a greenhouse of a scion-picking female parent;
when the plant grows to 6 leaves and 1 heart, removing the terminal bud to promote the lateral bud to sprout at the base of the leaf, and when the lateral bud grows to 4 leaves and 1 heart, removing the terminal bud;
and (4) topping and pinching, wherein after 6-8 weeks, the yield of the female parent reaches 2-3 cuttings/two weeks/plant, the female parent is established and planted, and the seedling production is formally carried out.
3. The method for asexual propagation of seedlings of eustoma grandiflorum according to claim 2, wherein the step of planting the scion female parent plant comprises: the planting substrate adopts a light soilless culture substrate coconut chaff.
4. The method for asexual propagation of seedlings of eustoma grandiflorum according to claim 2, wherein the step of planting the scion female parent plant comprises: the female parent cultivation greenhouse is controlled between 13 ℃ and 28 ℃, the illumination intensity is between 30000 and 50000Lx, and the relative air humidity is between 50 percent and 70 percent.
5. The method for asexual propagation of seedlings of eustoma grandiflorum according to claim 1, wherein the second step of cutting and harvesting comprises: selecting strong lateral buds from the established eustoma grandiflorum female parent plant, and harvesting cuttings with sterilized scissors; and (3) after cutting and harvesting, putting into a cutting and harvesting bag in time, spraying water in time to preserve moisture, putting into a refrigeration house with the temperature of 4-6 ℃ for standby, wherein the time is preferably not more than 24 hours, or immediately harvesting and inserting.
6. The method for asexual propagation of seedlings of eustoma grandiflorum according to claim 1, wherein the second step of cutting harvest comprises: each cutting retained 2 leaves 1 heart or 4 leaves 1 heart.
7. The asexual propagation seedling method of eustoma grandiflorum according to claim 1, wherein the third step of cuttage comprises:
preparing a seedling culture substrate: adopting a plug tray, and putting a seedling substrate block in the plug tray; the matrix block is made of peat: the perlite is mixed according to the volume ratio of 5: 1; before cutting, the substrate block fully absorbs water for later use;
cuttage: dipping the base part of the cutting into rooting powder, inserting the cutting into a seedling raising block until the insertion depth reaches the base part of the leaf;
the rooting powder is prepared by uniformly mixing IBA (indolebutyric acid) in talcum powder, and the IBA concentration is 2500 ppm.
8. The asexual propagation seedling method of eustoma grandiflorum according to claim 1, wherein the third step of cuttage comprises: dipping the base part of the cutting into rooting powder, inserting the cutting into a seedling raising block until the insertion depth reaches the base part of the leaf; the rooting powder is prepared by uniformly mixing IBA indolebutyric acid in talcum powder, and the concentration of IBA is 2500 ppm.
9. The method for asexual propagation of seedlings of eustoma grandiflorum according to claim 1, wherein the step of four-cuttage post-management comprises: adopting full light treatment, keeping the air humidity in the cuttage greenhouse above 85% by high-pressure mist intermittent spraying, and keeping the temperature of the seedling raising greenhouse at 24 ℃ all day; 5-7 days after cutting, callus appears on the base of the cutting shoot; after 10-14 days, the root system rapidly grows and extends out of the seedling raising block, and the seedling begins to enter a seedling hardening greenhouse for hardening seedlings.
10. The method for asexual propagation of seedlings of eustoma grandiflorum according to claim 1, wherein the step of seedling hardening comprises: during seedling exercising, the temperature is controlled to be between 18 and 21 ℃, the humidity is controlled to be between 70 and 85 percent, and the light is controlled to be 20000-25000 Lx; hardening the seedlings for 5 to 7 days until the height of the seedlings reaches 6 to 10cm, and germinating.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010115144A (en) * | 2008-11-12 | 2010-05-27 | Shimane Prefecture | Cultivation method for eustoma russellianum |
| CN103229701A (en) * | 2013-05-07 | 2013-08-07 | 云南省农业科学院花卉研究所 | Cutting propagation method of eustoma grandiflorum |
| CN110558216A (en) * | 2019-10-16 | 2019-12-13 | 云南省农业科学院花卉研究所 | Eustoma russellianum seedling growing method for eliminating rosette characters through temperature and light regulation |
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- 2022-05-27 CN CN202210593793.7A patent/CN115088489A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010115144A (en) * | 2008-11-12 | 2010-05-27 | Shimane Prefecture | Cultivation method for eustoma russellianum |
| CN103229701A (en) * | 2013-05-07 | 2013-08-07 | 云南省农业科学院花卉研究所 | Cutting propagation method of eustoma grandiflorum |
| CN110558216A (en) * | 2019-10-16 | 2019-12-13 | 云南省农业科学院花卉研究所 | Eustoma russellianum seedling growing method for eliminating rosette characters through temperature and light regulation |
Non-Patent Citations (1)
| Title |
|---|
| 林峰莹等: ""洋桔梗切花生产栽培"", 《云南农业》, no. 2, 30 January 2022 (2022-01-30) * |
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