CN103766039A - Method for breaking dormancy of nervilis fordii schlecht bulb - Google Patents
Method for breaking dormancy of nervilis fordii schlecht bulb Download PDFInfo
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- CN103766039A CN103766039A CN201410054628.XA CN201410054628A CN103766039A CN 103766039 A CN103766039 A CN 103766039A CN 201410054628 A CN201410054628 A CN 201410054628A CN 103766039 A CN103766039 A CN 103766039A
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- bulb
- ford nervilia
- nervilia leaf
- dormancy
- fordii
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- 230000005059 dormancy Effects 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 25
- 229940088597 hormone Drugs 0.000 claims abstract description 16
- 239000005556 hormone Substances 0.000 claims abstract description 16
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 241001314539 Nervilia Species 0.000 claims description 55
- 230000035784 germination Effects 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 10
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims description 10
- 229960001669 kinetin Drugs 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 4
- 230000000284 resting effect Effects 0.000 claims description 3
- 210000002615 epidermis Anatomy 0.000 claims description 2
- 244000037666 field crops Species 0.000 claims description 2
- 238000003475 lamination Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- 230000003020 moisturizing effect Effects 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 238000001953 recrystallisation Methods 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 2
- 241000233855 Orchidaceae Species 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 208000000616 Hemoptysis Diseases 0.000 description 1
- 241000174387 Nervilia fordii Species 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 201000007227 lymph node tuberculosis Diseases 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for breaking dormancy of a nervilis fordii schlecht bulb. The method comprises the following steps: 1, performing earlier stage treating on the nervilis fordii schlecht bulb; 2, preparing a hormone solution with a concentration of 1-250 ppm; 3, soaking the nervilis fordii schlecht bulb in a solution with a certain hormone concentration; 4, culturing the nervilis fordii schlecht bulb; and 5, transplanting. The method has the advantages that by adopting the method provided by the invention, a manner that nervilis fordii schlecht grows once is changed into a manner that the nervilis fordii schlecht grows twice, and thus the annual yield by area and the income of the nervilis fordii schlecht are increased, and a technical support is provided for increase of the yield of the nervilis fordii schlecht and the sustainable development.
Description
Technical field
The invention belongs to agronomy, biology, pharmaceutical field.Relate to a kind of method of breaking plant dormancy, a kind of specifically method of breaking the dormancy of ford nervilia leaf bulb,
Background technology
Ford nervilia leaf is the dried leaves of the orchid family (Orchidaceae) plant Nervilia (Nervilia) Nervilia fordii Nervilia fordii (Heance) Schltr. or the leaf with bulb, belongs to perennial root herbelet.Be distributed in the ground such as Guangxi, Guangdong, Sichuan, Yunnan.Cool in nature, taste is sweet, have moisten the lung and relieve the cough, invigorating the spleen disappears long-pending, clearing heat and detoxicating, blood stasis removing analgesic, cures mainly the diseases such as tuberculosis, cough hemoptysis, scrofula, bronchitis.Because the market demand is large and ford nervilia leaf self leans on propagation by corm, reproduction rate is extremely low, and the destruction of ecotope, is rare medicinal material in imminent danger at present.Therefore, improving the culture technique of ford nervilia leaf, increase the year output of ford nervilia leaf, is a kind of effective way that solves ford nervilia leaf MED SUP deficiency and sustainable use.But the culture technique of ford nervilia leaf is with propagation by corm at present, within 1 year, only grow once, and the general Only Six Months of growth time (March is then to August), bulb, in resting state, there is no bibliographical information at present for the dormancy of how to break ford nervilia leaf bulb At All Other Times.This patent is broken ford nervilia leaf dormancy, for further investigation, raising output and Sustainable Development and Utilization ford nervilia leaf provide reference.
Summary of the invention
The present invention provides a kind of method of breaking the dormancy of ford nervilia leaf bulb for making up the deficiencies in the prior art.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
A kind of method of breaking the dormancy of ford nervilia leaf bulb, aim to provide a kind of by breaking the dormancy of ford nervilia leaf bulb, allow ford nervilia leaf only grow and once to become 1 year secondary for 1 year, thereby improve annual yield by area and the income of ford nervilia leaf, for ford nervilia leaf root yield increases and sustainable development provides technical support.The method comprises the following steps:
1. process the early stage of ford nervilia leaf bulb
From the matrix of field or cultivation ford nervilia leaf, dig out in the full ford nervilia leaf bulb without mildew or stain of resting state, wash away the silt above bulb with running water, put on room table top and dry 5～10 hours, bulb epidermis is slightly dry anhydrous.
2. compound concentration is 1ppm～250ppm hormone solution
Compound method, first take out kinetin powder from the refrigerator of-20 ℃, accurately taking 0.001g～0.250g pours in test tube, then volumetric concentration 95% ethanol adding dissolves about 30 minutes～1 hour, after kinetin dissolves completely, more slowly pours in the graduated cylinder that fills 600～800ml distilled water (or pure water), predominate stirring on one side, avoid hormone to produce recrystallization, finally shake up to 1000ml with distilled water constant volume, join to obtain the concentration kinetin solution that is 1ppm～250ppm.
3. bulb soaks in hormone solution
The dormancy ford nervilia leaf bulb that step 1 is processed is put into and the hormone solution that step 2 prepares is housed is soaked, and hormone solution soaks all ford nervilia leaf dormancy bulbs completely.Solution concentration is respectively 1ppm, 2ppm, 5ppm, 10ppm, 20ppm, 40ppm, 80ppm and 250ppm, wherein better with 10ppm; Soak time is 5 minutes, 10 minutes, 30 minutes, 1 hour, 6 hours and 10 hours, is advisable with 10～30 minutes.
4. the cultivation of bulb
Soaked step 3 ford nervilia leaf bulb is put into clear glass culture dish, in glass dish, be placed with 2～3 layers and soaked pure water or the drenched filter paper of distilled water, ford nervilia leaf bulb is put on filter paper, bulb can not be piled up lamination, vessel cover upper cover, moisturizing, and be put in incubator and cultivate, incubator temperature is 18 ℃, 22 ℃, 25 ℃, 28 ℃, 31 ℃, wherein temperature 25 ℃～31 ℃ better, best with 28 ℃.In the process of cultivating, often observe, keep filter paper moistening, keep humidity 80～90%.
Above-mentioned incubation time: bulb is put in incubator and cultivates, time length is soaked the concentration of hormone solution and germination rate and bud growing state depending on bulb bulb and is determined, in 18 ℃ and 22 ℃ of incubators, cultivate, bulb breaking dormancy germinating time is more of a specified duration, more than ten days, just there is bud to grow from bulb, and poor growth.Relatively in the incubator of 25 ℃, 28 ℃ and 31 ℃, cultivate, bulb breaking dormancy germinating time was at 8～10 days, and 100% breaking dormancy germinates, and bud grows fine.
5. transplant
Incubation time just can be transplanted about 20 days, and now ford nervilia leaf germinates neatly, and seedling stalwartness, transplants land for growing field crops after soaking disinfection liquid.
Advantage of the present invention:
Raw material of the present invention be easy to get and nontoxic, method of operating is easy, cost is low.
2. bulb dormancy time of the present invention is short, germination rate is high, generally within 6-10 days, reaches and breaks the dormancy of ford nervilia leaf bulb, and germination rate is up to more than 95%.
3. tool of the present invention is stronger practicality and promotional value, application of the present invention, can make the cultivation of ford nervilia leaf from becoming annually 1 year twice, improves year yield per unit area of ford nervilia leaf and improves income, solves a kind of method that current ford nervilia leaf medicinal material is in short supply.
Embodiment
Below in conjunction with each single factor in the invention process, to breaking the test of ford nervilia leaf bulb dormancy, the invention will be further described.
1. the single factor experiment of soak time to ford nervilia leaf bulb breaking dormancy:
Be respectively in 10ppm kinetin solution after 5 minutes, 10 minutes, 30 minutes, 1 hour, 6 hours and 10 hours by ford nervilia leaf dormancy bulb soaking concentration, pour out and directly put on glassware filter paper, add a cover and be put into again in incubator, 25 ℃ of cultivations, observe bulb soak time on breaking the impact of bulb dormancy, in table 1.
Table 1 soak time is on breaking the impact of ford nervilia leaf bulb dormancy
From the result of the test of table 1, bulb soak time is little on breaking dormancy impact, and statistical analysis shows bulb germination rate and not remarkable (p > 0.05) of soak time relation, and soaking the short time can not need too of a specified duration.Therefore generally soak 10 minutes to 30 minutes, from the healthy and strong degree of result of the test germination regularity and bud, to soak 10 minutes for well.
2. soak the single factor experiment of variable concentrations solution to ford nervilia leaf bulb breaking dormancy:
Respectively bulb is immersed in 1ppm, 2ppm, 5ppm, 10ppm, 20ppm, 40ppm, 80ppm and 250ppm to 30 minutes, then puts in the incubator of 28 ℃ and cultivate, observe cultivation temperature on breaking the impact of ford nervilia leaf bulb, in table 2.
Table 2 hormon concentration is on breaking the impact of ford nervilia leaf bulb dormancy
From the result of the test of table 2, the 6th day bulb germination rate reach more than 90% concentration only have 10%, the 8 day except the germination rate of concentration 1% be 67, other concentration all reaches 100%.From the neat and stalwartness of germinateing, take 10ppm concentration as best, the germination rate of statistical analysis bulb and soaking concentration relation little (p > 0.05).Therefore, the kinetin concentration of breaking the dormancy of ford nervilia leaf bulb is advisable from consumption, the neat and stalwartness 5ppm～80ppm of germinateing, and better with 5ppm～10ppm again, 10ppm is best.
3. the single factor experiment of different cultivation temperature to ford nervilia leaf bulb breaking dormancy:
Respectively by bulb distilled water and in 10ppm hormone solution soaked ford nervilia leaf bulb put into the incubator that temperature setting is set to 18 ℃, 22 ℃, 25 ℃, 28 ℃, 31 ℃, 35 ℃, observe cultivation temperature on breaking the impact of ford nervilia leaf bulb, in table 3.
Table 3 incubator different temperatures is on breaking the impact of ford nervilia leaf bulb dormancy
From the result of the test of table 3, bulb shows in the germination of different temperatures after being immersed in clear water, within the 8th day, the germination rate of 18 ℃, 22 ℃ is still 0, the germination rate of 25 ℃, 28 ℃ and 31 ℃ is respectively 64%, 79% and 85%, and the dormancy that before 22 ℃, temperature should not be broken ford nervilia leaf is described, in 25 ℃, 28 ℃ and 31 ℃ of germination rates, reach more than 80% and only have 31 ℃, when temperature exceedes or equals 35 ℃ while cultivating, bulb is sent out corruption, mouldy cultivating deliquescing after 6 days, therefore should not cultivate 35 ℃ and above temperature.The contrary germination rate that soaks hormone solution 10ppm bulb, be that 18 ℃ and 22 ℃ of the 8th day germination rates are respectively 0 and 10% in cultivation temperature, but 25 ℃, 28 ℃ and 31 ℃ all reach 100%, illustrate and soak after certain density kinetin solution, as long as temperature is suitable, just energy breaking dormancy, germination rate reaches more than 95%, from table 3, take 25 ℃ and 28 ℃ as breaking the optimum temperature of bulb dormancy.
From above test, to break in the method for ford nervilia leaf dormancy, preferred plan is that the concentration of kinetin solution is 10ppm, ford nervilia leaf bulb soak time is 10 minutes, incubator temperature is cultivated under the condition of 28 ℃, breaks bulb dormancy time the shortest, sprouts neat, healthy and strong.
Claims (1)
1. a method of breaking the dormancy of ford nervilia leaf bulb, is characterized in that, method comprises the following steps:
1) process the early stage of ford nervilia leaf bulb
From the matrix of field or cultivation ford nervilia leaf, dig out in the full ford nervilia leaf bulb without mildew or stain of resting state bulb, wash away the silt above bulb with running water, put on room table top and dry 5～10 hours, bulb epidermis is slightly dry anhydrous;
2) compound concentration is 1ppm～250ppm hormone solution
Compound method, first take out kinetin powder from the refrigerator of-20 ℃, accurately taking 0.001g～0.250g pours in test tube, then adding volumetric concentration is that 95% ethanol dissolves 30 minutes～1 hour, after kinetin dissolves completely, more slowly pours in the graduated cylinder that fills 600～800ml distilled water or pure water, predominate stirring on one side, avoid hormone to produce recrystallization, finally shake up to 1000ml with distilled water constant volume, join to obtain the concentration kinetin solution that is 1ppm～250ppm;
3) bulb soaks in hormone solution
The dormancy ford nervilia leaf bulb of step 1) processing is put into step 2 is housed) preparation hormone solution soak, hormone solution soaks all ford nervilia leaf dormancy bulbs completely, solution concentration is respectively 1ppm, 2ppm, 5ppm, 10ppm, 20ppm, 40ppm, 80ppm and 250ppm, wherein better with 10ppm; Soak time is 5 minutes, 10 minutes, 30 minutes, 1 hour, 6 hours and 10 hours, is advisable with 10～30 minutes;
4) cultivation of bulb
Soaked step 3) ford nervilia leaf bulb is put into clear glass culture dish, in glass dish, be placed with 2～3 layers and soaked pure water or the drenched filter paper of distilled water, ford nervilia leaf bulb is put on filter paper, bulb can not be piled up lamination, vessel cover upper cover, moisturizing, and be put in incubator and cultivate, the time is 6～20 days, incubator temperature is 18 ℃, 22 ℃, 25 ℃, 28 ℃, 31 ℃, wherein temperature at 25 ℃～31 ℃ better, with 28 ℃ best, cultivate process in, often observe, keep filter paper moistening, keep humidity 80～90%;
Described incubation time: bulb is put in incubator and cultivates, time length is soaked the concentration of hormone solution and germination rate and bud growing state depending on bulb bulb and is determined, in 18 ℃ and 22 ℃ of incubators, cultivate, bulb breaking dormancy germinating time is more of a specified duration, within ten days, just has above bud to grow from bulb, and poor growth, relatively in the incubator of 25 ℃, 28 ℃ and 31 ℃, cultivate, bulb breaking dormancy germinating time was at 8～10 days, and 100% breaking dormancy germinates, and bud grows fine;
5) transplant
Incubation time just can be transplanted at 20 days, and now ford nervilia leaf germinates neatly, and seedling stalwartness, transplants land for growing field crops after soaking disinfection liquid.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104067936A (en) * | 2014-06-18 | 2014-10-01 | 广西壮族自治区药用植物园 | Method for promoting nervilia fordii corms to grow more leaf buds |
| CN104221701A (en) * | 2014-10-20 | 2014-12-24 | 黄振忠 | Ford nervilia leaf artificial cultivation method |
| CN104737672A (en) * | 2015-03-20 | 2015-07-01 | 甘肃省治沙研究所 | Method for laying filter paper in culture dishes |
| CN105265289A (en) * | 2014-07-02 | 2016-01-27 | 北京中农绿源工程技术有限公司 | Four-season production method of gynura bicolor |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1872282A (en) * | 2006-01-13 | 2006-12-06 | 广州中医药大学 | Active extractive of Foliumnerviliae, preparation method and application |
| CN102134561A (en) * | 2010-12-30 | 2011-07-27 | 广东南台药业有限公司 | Culture medium used for fast breeding tissue-culture root-shaped stems of nervilia fordii |
| CN103535275A (en) * | 2012-07-15 | 2014-01-29 | 云南省德宏热带农业科学研究所 | Tissue-culture rapid multiplication method of nerviliae fordii corm |
-
2014
- 2014-02-18 CN CN201410054628.XA patent/CN103766039B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1872282A (en) * | 2006-01-13 | 2006-12-06 | 广州中医药大学 | Active extractive of Foliumnerviliae, preparation method and application |
| CN102134561A (en) * | 2010-12-30 | 2011-07-27 | 广东南台药业有限公司 | Culture medium used for fast breeding tissue-culture root-shaped stems of nervilia fordii |
| CN103535275A (en) * | 2012-07-15 | 2014-01-29 | 云南省德宏热带农业科学研究所 | Tissue-culture rapid multiplication method of nerviliae fordii corm |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104067936A (en) * | 2014-06-18 | 2014-10-01 | 广西壮族自治区药用植物园 | Method for promoting nervilia fordii corms to grow more leaf buds |
| CN104067936B (en) * | 2014-06-18 | 2016-04-27 | 广西壮族自治区药用植物园 | A kind of method impelling ford nervilia leaf bulb how long leaf bud |
| CN105265289A (en) * | 2014-07-02 | 2016-01-27 | 北京中农绿源工程技术有限公司 | Four-season production method of gynura bicolor |
| CN104221701A (en) * | 2014-10-20 | 2014-12-24 | 黄振忠 | Ford nervilia leaf artificial cultivation method |
| CN104737672A (en) * | 2015-03-20 | 2015-07-01 | 甘肃省治沙研究所 | Method for laying filter paper in culture dishes |
| CN104737672B (en) * | 2015-03-20 | 2018-10-02 | 甘肃省治沙研究所 | A kind of method of culture dish place mat filter paper |
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Application publication date: 20140507 Assignee: Guangxi Totuweisen Agricultural Technology Co.,Ltd. Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS Contract record no.: X2023980045866 Denomination of invention: A Method of Breaking the Dormancy of Green Sky Sunflower Bulbs Granted publication date: 20150603 License type: Common License Record date: 20231107 |
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